MS002399 (gene) Bitter gourd (TR) v1

Overview


Name	MS002399
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	EamA domain-containing protein
Location	scaffold30: 4596274 .. 4599039 (-)
RNA-Seq Expression	MS002399
Synteny	MS002399

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTGGAGGTACAAGGGAGGGCTGATTCTTCTCATCGCTGTAGTAGTCATATGGGTTTCCTCTGCAGAGATCACACAGGTAATTAATTATCTCATATCGTTCATAGATTTTTGATATTGTGTCTAATAAATCCCTAAACTTTAAAAAGATTCCAAATAAGTATCTAAAATTTTTAATTTTGCATCTAAAGAAACTGTCATTAGTCACAAAATTGAAACTTCAAGAATTTATTAAATTAACCATTTAATTTCTATTCTTACTATTTTTAACACTTTTATGAGCTTGAAAATTAGCACAAGACCCAACAAATGTACTATTATATAAAATGAAGAAATGAGAGTATCCTATAGATCAGATTCTAACTATGCATGATAAATTATCACTAGACTCAAAAGCTTGAGCGGATATATTACCGTAGTTAATTTTTATTATTTCTCTGTGTTGGTAGAGTATTTTCAGGGACTACGAGCACCCATTTGTGATGACATACATAGGAACCTCCATGTTGGTGGCTTATCTTGCAATAGCATTCATCAAAGAATGCATAGTGAAGCTGTTCAGAACCCATTTTTCAAATGGAAACTCCAAAAAAGTTGCAGAGGTAGCCCAGCCACAAGTCATGGAAGAAAAAAGCAATAACCCCTCTTCTCCAAATAATAATAATAATAATAATATATCAGAGGTGGGAGATGGATCAGAAGTTCATTGTGTGGTGCAAATTCAATGTGAAGAAGAAGTTGTGAGTGTTGATAAAGATAACTATGGTGGCTGTGAATGTGAAAATCAAGAAGTGGCAATGGCGTTGAAAACAAAGGAAACTAAGTTTACTACAAAGCAAATTGCTGTCTTGGCTCTCACCATTGGCCCCATTTGGTTTGTTTCTGAGGTTAGTGACTCGGGTTCTATTTTTTTGTAACGTTTTTGTTTCTTCATTTTTAATAAAGAAAAATGAATTTGCTGATTTAACATGAATTCAAATATGATAAAACAATTTACTTATGTTTGGGAACAAATTGAAAATATGAGCACCCTCAACTTTCTTAATTTTGTTGTCATTGAATTTCTTTTTTTCTAACTTGGAGTAGTAAAGACCAAAATAGAGGGGTTGAAAATGTTTAATGAAGTTGTTTTGAAAGTTTAAAGACCAAAATGAAATAATTAAATGGTGGAAATGTGTGGATGCAGTATTTTACAAATGCAGCACTGGCAAGAACAAGCGTAGCCACGACCACCATATTGTTCTCAACATCAGGGCTGTTCACACTCATAATAGACGCATTCTTGGAAAGGCAATCTTTAACAATCGTAAATATTGTCGCCGTCGTCGTCAGTCTCGCCGGCGTCGCCATGACAACCGTCGGCAAGACTTGGGCCAAAGACCAACCCCACTCCACTTCCTCTGAGTACGCTTCTTCTCTTCTCCTCTCTATTTTTTGCTTTCAAATTGGCTTTATAAATTGTAATTTGAACTCATATTCAACTGTTCTTAGAGGGCACACTCGTAACATCCCATGGCAACATTCACAATAAATAATTAAATAATTATTTTAAAAAAGATCCTGAGCTTAAACTCTCCGCTAACCATAATTTTAAATGTTAAGGTCTCAATTTTGTGGAAATATCGATAAAATGTATGATAGTTAGAGAAGTTAAAAAAATCGCAAAAAATATTTAAACTAGCAAATAAATATTTTCTCTATTTATAAGTAAGTTAAAAATGATTAATATTAATATTATGTATATATCAGTGACATTTTGTTGATATTTTTCATATTTTATCGATTTTTTTGTACTACGATGAAAATGTCAATTTATCCTTCATGCTAAGGTGGAATCGATAGATACATAGACATGTCAATAGAAATGTCAACCTGTCGACGACATTTAATACCATGCCGCTAACATATCGTTAATTATTTATTTATTTATCTGGGAACTTATTTTCAGGCATGGAAAGCACTCATTCGTTGGAGATGCATTTGCTCTGCTCTCAGCCCTCACCGATGGGCTTTACTACGGTTGGTGGCTGCTGCTTCCTTTTTAGTTTAGTTTGGTTGGTTATTTTATTTATTTATTTATTTATTTTGCTAATTAAATATTTTTCTTTGAATTAATTGCAAATAAATGAAATGGTAATAATTAATTATGGAACATTTTCATGATCGGAAAAAGAAATTGTGTGCAGTGCTTTTGAAGAAGTACGCAGGGGAAGAAGGGGAAAAAATTGACATGCAGAAATTTCTTGGCTACGTTGGATTATTCACTCTCACTACTCTCTGGTGGCTAAGTAAGAACATCAAAATCAAATTAAATTAAATTAAAAAAACACCTCTCTCTCTCTCTCTCTCTAAATTCTCACATTTGGTTTTGTTTTTTTCAGTATGGCCATTGAGAGCCATTGGAATAGAGCCCAAATTTGCAATCCCACGGTCCACAAAAGTGGCAGAACTTGTGCTTGCCAATTGCTTCATAGGAAACTTCCTTTCCGATTACTTCTGGTTCGTACATCAGAATAATTTTGCGCCGTTCGATGTATGAAATTTATCATGCATCTAACGGTTAGGAAAGTGACAAAACATTTTTATTACGAAATATTAAGATGAGAGAAATGGTTGTAGGGCAATGGGCGTTGTTTGGACGAGTCCACTTGTAGCTGCTTTAGGTGCTTCTCTAACGATACCACTCGCCATGTTGGGAGACATGGTCCTACATGGCCGTCATTATTCTTTGGTTTACATTTTCGGATCCCTTCAAGTAAGTATT

mRNA sequence

ATGGCGTGGAGGTACAAGGGAGGGCTGATTCTTCTCATCGCTGTAGTAGTCATATGGGTTTCCTCTGCAGAGATCACACAGAGTATTTTCAGGGACTACGAGCACCCATTTGTGATGACATACATAGGAACCTCCATGTTGGTGGCTTATCTTGCAATAGCATTCATCAAAGAATGCATAGTGAAGCTGTTCAGAACCCATTTTTCAAATGGAAACTCCAAAAAAGTTGCAGAGGTAGCCCAGCCACAAGTCATGGAAGAAAAAAGCAATAACCCCTCTTCTCCAAATAATAATAATAATAATAATATATCAGAGGTGGGAGATGGATCAGAAGTTCATTGTGTGGTGCAAATTCAATGTGAAGAAGAAGTTGTGAGTGTTGATAAAGATAACTATGGTGGCTGTGAATGTGAAAATCAAGAAGTGGCAATGGCGTTGAAAACAAAGGAAACTAAGTTTACTACAAAGCAAATTGCTGTCTTGGCTCTCACCATTGGCCCCATTTGGTTTGTTTCTGAGTATTTTACAAATGCAGCACTGGCAAGAACAAGCGTAGCCACGACCACCATATTGTTCTCAACATCAGGGCTGTTCACACTCATAATAGACGCATTCTTGGAAAGGCAATCTTTAACAATCGTAAATATTGTCGCCGTCGTCGTCAGTCTCGCCGGCGTCGCCATGACAACCGTCGGCAAGACTTGGGCCAAAGACCAACCCCACTCCACTTCCTCTGAGCATGGAAAGCACTCATTCGTTGGAGATGCATTTGCTCTGCTCTCAGCCCTCACCGATGGGCTTTACTACGTGCTTTTGAAGAAGTACGCAGGGGAAGAAGGGGAAAAAATTGACATGCAGAAATTTCTTGGCTACGTTGGATTATTCACTCTCACTACTCTCTGGTGGCTAATATGGCCATTGAGAGCCATTGGAATAGAGCCCAAATTTGCAATCCCACGGTCCACAAAAGTGGCAGAACTTGTGCTTGCCAATTGCTTCATAGGAAACTTCCTTTCCGATTACTTCTGGGCAATGGGCGTTGTTTGGACGAGTCCACTTGTAGCTGCTTTAGGTGCTTCTCTAACGATACCACTCGCCATGTTGGGAGACATGGTCCTACATGGCCGTCATTATTCTTTGGTTTACATTTTCGGATCCCTTCAAGTAAGTATT

Coding sequence (CDS)

ATGGCGTGGAGGTACAAGGGAGGGCTGATTCTTCTCATCGCTGTAGTAGTCATATGGGTTTCCTCTGCAGAGATCACACAGAGTATTTTCAGGGACTACGAGCACCCATTTGTGATGACATACATAGGAACCTCCATGTTGGTGGCTTATCTTGCAATAGCATTCATCAAAGAATGCATAGTGAAGCTGTTCAGAACCCATTTTTCAAATGGAAACTCCAAAAAAGTTGCAGAGGTAGCCCAGCCACAAGTCATGGAAGAAAAAAGCAATAACCCCTCTTCTCCAAATAATAATAATAATAATAATATATCAGAGGTGGGAGATGGATCAGAAGTTCATTGTGTGGTGCAAATTCAATGTGAAGAAGAAGTTGTGAGTGTTGATAAAGATAACTATGGTGGCTGTGAATGTGAAAATCAAGAAGTGGCAATGGCGTTGAAAACAAAGGAAACTAAGTTTACTACAAAGCAAATTGCTGTCTTGGCTCTCACCATTGGCCCCATTTGGTTTGTTTCTGAGTATTTTACAAATGCAGCACTGGCAAGAACAAGCGTAGCCACGACCACCATATTGTTCTCAACATCAGGGCTGTTCACACTCATAATAGACGCATTCTTGGAAAGGCAATCTTTAACAATCGTAAATATTGTCGCCGTCGTCGTCAGTCTCGCCGGCGTCGCCATGACAACCGTCGGCAAGACTTGGGCCAAAGACCAACCCCACTCCACTTCCTCTGAGCATGGAAAGCACTCATTCGTTGGAGATGCATTTGCTCTGCTCTCAGCCCTCACCGATGGGCTTTACTACGTGCTTTTGAAGAAGTACGCAGGGGAAGAAGGGGAAAAAATTGACATGCAGAAATTTCTTGGCTACGTTGGATTATTCACTCTCACTACTCTCTGGTGGCTAATATGGCCATTGAGAGCCATTGGAATAGAGCCCAAATTTGCAATCCCACGGTCCACAAAAGTGGCAGAACTTGTGCTTGCCAATTGCTTCATAGGAAACTTCCTTTCCGATTACTTCTGGGCAATGGGCGTTGTTTGGACGAGTCCACTTGTAGCTGCTTTAGGTGCTTCTCTAACGATACCACTCGCCATGTTGGGAGACATGGTCCTACATGGCCGTCATTATTCTTTGGTTTACATTTTCGGATCCCTTCAAGTAAGTATT

Protein sequence

MAWRYKGGLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAIAFIKECIVKLFRTHFSNGNSKKVAEVAQPQVMEEKSNNPSSPNNNNNNNISEVGDGSEVHCVVQIQCEEEVVSVDKDNYGGCECENQEVAMALKTKETKFTTKQIAVLALTIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVVVSLAGVAMTTVGKTWAKDQPHSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKIDMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSDYFWAMGVVWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYIFGSLQVSI

Homology

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BLAST of MS002399 vs. NCBI nr
Match: XP_022134812.1 (uncharacterized transporter C405.03c-like [Momordica charantia])

HSP 1 Score: 735.3 bits (1897), Expect = 2.7e-208
Identity = 383/390 (98.21%), Postives = 384/390 (98.46%), Query Frame = 0


Query: 1   MAWRYKGGLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAIAFIKECI 60
           MAWRYKGGLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAIAFIKECI
Sbjct: 1   MAWRYKGGLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAIAFIKECI 60

Query: 61  VKLFRTHFSNGNSKKVAEVAQPQVMEEKSNNPSSP-NNNNNNNISEVGDGSEVHCVVQIQ 120
           VKLFRTHFSNGNSKKVAEVAQPQVMEEKSNNPSSP NNNNNNNISEVGDGSEVHCVVQIQ
Sbjct: 61  VKLFRTHFSNGNSKKVAEVAQPQVMEEKSNNPSSPNNNNNNNNISEVGDGSEVHCVVQIQ 120

Query: 121 CEEEVVSVDKDNYGGCECENQEVAMALKTKETKFTTKQIAVLALTIGPIWFVSEYFTNAA 180
           CEEEVVSVDKDNYGGCECENQEVAMALKTKETKF     AVLALTIGPIWFVSEYFTNAA
Sbjct: 121 CEEEVVSVDKDNYGGCECENQEVAMALKTKETKF-----AVLALTIGPIWFVSEYFTNAA 180

Query: 181 LARTSVATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVVVSLAGVAMTTVGKTWAKDQ 240
           LARTSVATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVVVSLAGVAMTT+GKTWAKDQ
Sbjct: 181 LARTSVATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVVVSLAGVAMTTIGKTWAKDQ 240

Query: 241 PHSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKIDMQKFLGYVGLFTLTT 300
           PHSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKIDMQKFLGYVGLFTLTT
Sbjct: 241 PHSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKIDMQKFLGYVGLFTLTT 300

Query: 301 LWWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSDYFWAMGVVWTSPLVAALGA 360
           LWWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSDYFWAMGVVWTSPLVAALGA
Sbjct: 301 LWWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSDYFWAMGVVWTSPLVAALGA 360

Query: 361 SLTIPLAMLGDMVLHGRHYSLVYIFGSLQV 390
           SLTIPLAMLGDMVLHGRHYSLVYIFGSLQV
Sbjct: 361 SLTIPLAMLGDMVLHGRHYSLVYIFGSLQV 385

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BLAST of MS002399 vs. NCBI nr
Match: XP_038880237.1 (uncharacterized vacuolar membrane protein YML018C-like [Benincasa hispida])

HSP 1 Score: 605.9 bits (1561), Expect = 2.4e-169
Identity = 318/397 (80.10%), Postives = 355/397 (89.42%), Query Frame = 0


Query: 1   MAWRYKGGLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAIAFIKECI 60
           MAW+YK GLILL+AVVVIWV+SAEITQSIF DYEHPFV+TY+GTSMLVAYLAIAFIKECI
Sbjct: 1   MAWKYKVGLILLVAVVVIWVASAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60

Query: 61  VKLFRTHFSNGNSKKVAEVAQP-QVMEEKSNNPS------SPNNNNNNNISEVGDGSEVH 120
           VK+FR+HF NGNS+KVAE+  P   +EE++N  S      + NNNNNN ISEVG+G +V 
Sbjct: 61  VKIFRSHFRNGNSRKVAELQPPLPALEERTNQSSIEDDDNNNNNNNNNIISEVGNG-DVQ 120

Query: 121 CVVQIQCEEEVVSVDKDNYGGCECENQEVAMA-LKTKETKFTTKQIAVLALTIGPIWFVS 180
           CVV I   E++   + ++ GGCECE + + M  LKT+ETK +TKQIAVLAL IGPIWFVS
Sbjct: 121 CVVNIIQGEDLRVNNNNHNGGCECEPENLEMTILKTEETKCSTKQIAVLALVIGPIWFVS 180

Query: 181 EYFTNAALARTSVATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVVVSLAGVAMTTVG 240
           EYFTNAALARTSVATTTILFSTSGLFTLIIDA LERQSLTIVN+VAV+VS+AGVAMTTVG
Sbjct: 181 EYFTNAALARTSVATTTILFSTSGLFTLIIDACLERQSLTIVNVVAVIVSMAGVAMTTVG 240

Query: 241 KTWAKDQPHSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKIDMQKFLGYV 300
           KTWA+D+  S+SSEHGKHS+VGD FALLSALTDGLYYVLLKKYAGEEGEK+DMQKFLGYV
Sbjct: 241 KTWARDEVRSSSSEHGKHSYVGDIFALLSALTDGLYYVLLKKYAGEEGEKVDMQKFLGYV 300

Query: 301 GLFTLTTLWWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSDYFWAMGVVWTSP 360
           GLFTLTTLWWLIWPLRAIGIEPKF IP+STKVAELVLANCF+ NF+SDYFWAMGVVWTSP
Sbjct: 301 GLFTLTTLWWLIWPLRAIGIEPKFMIPQSTKVAELVLANCFVSNFVSDYFWAMGVVWTSP 360

Query: 361 LVAALGASLTIPLAMLGDMVLHGRHYSLVYIFGSLQV 390
           LVAALGASLTIPLAMLGDMVLHGRHYSL+YIFGS+QV
Sbjct: 361 LVAALGASLTIPLAMLGDMVLHGRHYSLIYIFGSVQV 396

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BLAST of MS002399 vs. NCBI nr
Match: XP_023531720.1 (uncharacterized transporter C405.03c-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 586.6 bits (1511), Expect = 1.5e-163
Identity = 307/389 (78.92%), Postives = 342/389 (87.92%), Query Frame = 0


Query: 1   MAWRYKGGLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAIAFIKECI 60
           MAW+YKGGLILL+AVVVIWV+SAEITQSIF DYEHPFVMTY+GTSMLVAYLAIAFI+ECI
Sbjct: 1   MAWKYKGGLILLVAVVVIWVASAEITQSIFTDYEHPFVMTYVGTSMLVAYLAIAFIRECI 60

Query: 61  VKLFRTHFSNGNSKKVAEVAQPQVMEEKSNNPSSPNNNNNNNISEVGDGSEVHCVVQIQC 120
           VKLFR+HF N NSK+VAE+ Q Q     +NN ++ NNNNNNNISEV +G +VHCVV I+C
Sbjct: 61  VKLFRSHFPNRNSKEVAEI-QEQDNNNNNNNTNNNNNNNNNNISEVENG-DVHCVVNIEC 120

Query: 121 EEEVVSVDKDNYGGCECENQEVAMALKTKETKFTTKQIAVLALTIGPIWFVSEYFTNAAL 180
           EE+       N G CECE +       T+ET F+TKQ+AVLAL IGPIWFVSEYFTNAAL
Sbjct: 121 EEQEKG-SVSNNGRCECEAEN-----GTQETPFSTKQMAVLALAIGPIWFVSEYFTNAAL 180

Query: 181 ARTSVATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVVVSLAGVAMTTVGKTWAKDQP 240
           ARTSVATTTILFSTSGLFTLI+DA  ERQSL+IVN+VAV VS+AGVAMTTVGKTWA+D+ 
Sbjct: 181 ARTSVATTTILFSTSGLFTLILDACFERQSLSIVNVVAVFVSMAGVAMTTVGKTWARDET 240

Query: 241 HSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKIDMQKFLGYVGLFTLTTL 300
            S S  H KHS++GD FALLSA TDGLYYVLLKKYAGEEGEK+DMQKFLGYVGLFTLTTL
Sbjct: 241 QS-SFGHEKHSYIGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTLTTL 300

Query: 301 WWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSDYFWAMGVVWTSPLVAALGAS 360
           WWLIWPLRA+GIEPKF IP+STKVAE+VLANCF+ NF+SDYFWAMGVVWTSPLVAALGAS
Sbjct: 301 WWLIWPLRAMGIEPKFGIPQSTKVAEIVLANCFVSNFVSDYFWAMGVVWTSPLVAALGAS 360

Query: 361 LTIPLAMLGDMVLHGRHYSLVYIFGSLQV 390
           LTIPLAM+GDM+LHGRHYSLVYIFGSLQV
Sbjct: 361 LTIPLAMVGDMLLHGRHYSLVYIFGSLQV 380

[Close]

BLAST of MS002399 vs. NCBI nr
Match: TYK06345.1 (putative transporter-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 583.9 bits (1504), Expect = 1.0e-162
Identity = 309/393 (78.63%), Postives = 342/393 (87.02%), Query Frame = 0


Query: 1   MAWRYKGGLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAIAFIKECI 60
           MAW+YK GLILL+AVVVIWV+SAEITQSIF DYEHPFV+TY+GTSMLVAYLAIAFIKECI
Sbjct: 1   MAWKYKVGLILLVAVVVIWVTSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60

Query: 61  VKLFRTHF-SNGNSKKVAEVAQPQVMEEKSNNPSSPNNNNNNNISEVGDGSEVHCVVQIQ 120
           +K+ R+HF + GN +KVAE+  P    E+  N SS  NNNNN  S+  +  +V  VV I 
Sbjct: 61  MKIVRSHFRTKGNCRKVAEIQSPLPALEERTNQSS--NNNNNTTSDKVENRDVQYVVNIN 120

Query: 121 CEEEVVSVDKDNYGGC---ECENQEVAMALKTKETKFTTKQIAVLALTIGPIWFVSEYFT 180
                 +   +N GGC   E EN E+ + LKT+E KF+TKQIAVLALTIGPIWFVSEYFT
Sbjct: 121 DNNNNSNNSSNNNGGCELREAENMEMRI-LKTEEAKFSTKQIAVLALTIGPIWFVSEYFT 180

Query: 181 NAALARTSVATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVVVSLAGVAMTTVGKTWA 240
           NAALARTSVATTTILFSTSGLFTLI+DA LERQSLTIVN+VAVVVS+AGVAMTTVGKTWA
Sbjct: 181 NAALARTSVATTTILFSTSGLFTLILDACLERQSLTIVNVVAVVVSMAGVAMTTVGKTWA 240

Query: 241 KDQPHSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKIDMQKFLGYVGLFT 300
           KD+P S+S  HGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEK+DMQKFLGYVGLFT
Sbjct: 241 KDEPQSSSYGHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFT 300

Query: 301 LTTLWWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSDYFWAMGVVWTSPLVAA 360
           LTTLWWLIWPLRAIGIEPKF IP+STKVAE+VLANCF+ NF+SDYFWAMGVVWTSPLVAA
Sbjct: 301 LTTLWWLIWPLRAIGIEPKFMIPQSTKVAEVVLANCFVANFVSDYFWAMGVVWTSPLVAA 360

Query: 361 LGASLTIPLAMLGDMVLHGRHYSLVYIFGSLQV 390
           LGASLTIPLAM+GDMVLHGRHYSLVYIFGS+QV
Sbjct: 361 LGASLTIPLAMIGDMVLHGRHYSLVYIFGSIQV 390

[Close]

BLAST of MS002399 vs. NCBI nr
Match: XP_022925420.1 (uncharacterized transporter C405.03c-like [Cucurbita moschata])

HSP 1 Score: 583.2 bits (1502), Expect = 1.7e-162
Identity = 302/389 (77.63%), Postives = 341/389 (87.66%), Query Frame = 0


Query: 1   MAWRYKGGLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAIAFIKECI 60
           MAW+YKGGLILL+AVVVIWV+SAEITQSIF DYEHPFVMTY+GTSMLVAYLAIAF++ECI
Sbjct: 1   MAWKYKGGLILLVAVVVIWVASAEITQSIFTDYEHPFVMTYVGTSMLVAYLAIAFVRECI 60

Query: 61  VKLFRTHFSNGNSKKVAEVAQPQVMEEKSNNPSSPNNNNNNNISEVGDGSEVHCVVQIQC 120
           VKLFR+HF NGN K+VAE+      +E+ NN ++ NNNNNNNISEV +G +VHCVV ++C
Sbjct: 61  VKLFRSHFPNGNPKEVAEI------QEQDNNNNNNNNNNNNNISEVENG-DVHCVVNVEC 120

Query: 121 EEEVVSVDKDNYGGCECENQEVAMALKTKETKFTTKQIAVLALTIGPIWFVSEYFTNAAL 180
           EE+       N G CECE +       T+ET F+TKQ+AVLAL IGPIWFVSEYFTNAAL
Sbjct: 121 EEQEKG-SASNNGRCECEAEN-----GTQETSFSTKQMAVLALAIGPIWFVSEYFTNAAL 180

Query: 181 ARTSVATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVVVSLAGVAMTTVGKTWAKDQP 240
           A+TSVATTTILFSTSGLFTLI+DA  ERQSL+IVN+VAV VS+AGVAMTTVGKTWA+D+ 
Sbjct: 181 AKTSVATTTILFSTSGLFTLILDACFERQSLSIVNVVAVFVSMAGVAMTTVGKTWARDEA 240

Query: 241 HSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKIDMQKFLGYVGLFTLTTL 300
            S S  H KHS++GD FALLSA TDGLYYVLLKKYAGEEGEK+DMQKFLGYVGLFTLTTL
Sbjct: 241 QS-SFGHEKHSYIGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTLTTL 300

Query: 301 WWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSDYFWAMGVVWTSPLVAALGAS 360
           WWLIWPLRAIGIEPKF +P+STKVAE+VLANCF+ NF+SDY WAMGVVWTSPLVAALGAS
Sbjct: 301 WWLIWPLRAIGIEPKFGLPQSTKVAEIVLANCFVSNFVSDYLWAMGVVWTSPLVAALGAS 360

Query: 361 LTIPLAMLGDMVLHGRHYSLVYIFGSLQV 390
           LTIPLAM+GDM+LHGRHYSLVYIFGSLQV
Sbjct: 361 LTIPLAMVGDMLLHGRHYSLVYIFGSLQV 375

[Close]

BLAST of MS002399 vs. ExPASy Swiss-Prot
Match: Q03730 (Uncharacterized vacuolar membrane protein YML018C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YML018C PE=1 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.7e-35
Identity = 124/389 (31.88%), Postives = 189/389 (48.59%), Query Frame = 0


Query: 4   RYKGGLILLIAVVVIWVSSAEITQSIFRD--YEHPFVMTYIGTSMLVAYLAIAFIKECIV 63
           R+  GL++L  V+++WV S+ +   IF D  Y  PF +TY  T+  + YL     K  +V
Sbjct: 12  RWTLGLLMLGLVIILWVLSSFLINLIFEDDSYRKPFFITYTNTAAFIFYL-FPTAKAVVV 71

Query: 64  KLFRTHFSNGNSKKVAEVAQPQVMEEKSNNPSSPNNNNNNNISEVGDGSEVHCVVQIQCE 123
                   N      A V +  +MEE+                  G GS+ +  V     
Sbjct: 72  --------NYKDTGRANVHRELIMEEE------------------GTGSDSNRSV----- 131

Query: 124 EEVVSVDKDNYGGCECENQEVAMALKTKETKFTTKQIAVLALTIGPIWFVSEYFTNAALA 183
            ++ S    N       NQ+  + L   ET   + +  +L       WF +   TNA+LA
Sbjct: 132 -DMTSPLLTNLEAGTHANQKKRLTL--YETIKLSAEFCIL-------WFTANLVTNASLA 191

Query: 184 RTSVATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVVVSLAGVAMTTVGKTWAKDQPH 243
            TSVA+ TIL +TS  FTL I A    +SL+   ++   +S  G+ M T   +  + Q H
Sbjct: 192 FTSVASQTILSTTSSFFTLFIGAICHVESLSKSKVLGSFISFVGIIMVTKSDSHQRYQRH 251

Query: 244 ----STSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKIDMQKFLGYVGLFTL 303
               S          +G+  AL  A+  G+Y  LLK+  G+E  +++M+ F G+VGLF L
Sbjct: 252 IADVSGDDNDAVQVLIGNLLALAGAVLYGVYSTLLKREVGDE-TRVNMKIFFGFVGLFNL 311

Query: 304 TTLWWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSDYFWAMGVVWTSPLVAAL 363
             LW  +  L   G EP F++P+  KV  ++  NC I  F+SD+ WA  ++ TSPL   +
Sbjct: 312 LFLWPSLIVLDFFGWEP-FSLPKDPKVVVIIFVNCLI-TFVSDFCWAKAMLLTSPLTVTV 355

Query: 364 GASLTIPLAMLGDMVLHGRHYSLVYIFGS 387
           G S+TIPLAM GD++   +  S +Y+FG+
Sbjct: 372 GLSITIPLAMFGDVIFKHKTMSALYLFGA 355

[Close]

BLAST of MS002399 vs. ExPASy Swiss-Prot
Match: A6QL92 (Solute carrier family 35 member F5 OS=Bos taurus OX=9913 GN=SLC35F5 PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 3.0e-29
Identity = 119/406 (29.31%), Postives = 198/406 (48.77%), Query Frame = 0


Query: 8   GLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAIAFI-----KECIVK 67
           G+++L+ V VIWV+S+E+T  +F  Y  PF  T+  TSM V YL + FI     ++   +
Sbjct: 72  GIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVLYL-LGFIVWKPWRQQCTR 131

Query: 68  LFR----THFSNGNSKKVA---------EVAQPQVMEEKSNN-PSSPNNNNNNNISEVGD 127
            FR    T F++      A          +++P  +  K ++ PS    N N +  ++  
Sbjct: 132 GFRGKHATFFADAEGYFAACTTDTTMNSSLSEPLYVPVKFHDLPSEKPENTNIDTEKIPK 191

Query: 128 GSEVHC--VVQIQCEEEVVSVDKDNYGGCECENQEVAMALKTKETKFTTKQIAVLALTIG 187
            S V    +++I+      +++           +E    LKT   K T  Q+A ++    
Sbjct: 192 KSRVRFSNIMEIRQLPSSHALEAKLSRMSYPTVKEQESLLKT-VGKLTATQVAKISFFFC 251

Query: 188 PIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDAFLERQS---LTIVNIVAVVVSL 247
            +WF++ +    AL+ T VA   IL STSGLFTLI+ A     S    T+  ++AV++S+
Sbjct: 252 FVWFLANFSYQEALSDTQVAIVNILSSTSGLFTLILAAMFPSNSGDRFTLSKLLAVILSI 311

Query: 248 AGVAMTTVGKTWAKDQPHSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKI 307
            GV +  +          S S +    + +G  ++L+ A+   +Y V++K+    E +K+
Sbjct: 312 GGVVLVNL----------SGSEKSPGRNTIGSIWSLVGAMLYAVYIVMIKRKVDRE-DKL 371

Query: 308 DMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSDYFW 367
           D+  F G+VGLF L  LW   + L   G E  F  P    V   ++ N  IG  LS++ W
Sbjct: 372 DIPMFFGFVGLFNLLLLWPGFFLLHYTGFE-DFEFPNKV-VLMCIVINGLIGTVLSEFLW 431

Query: 368 AMGVVWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYIFGSLQV 390
             G   TS L+  L  SLTIPL+++ DM +    +S ++  G++ V
Sbjct: 432 LWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQFSWLFFAGAIPV 462

[Close]

BLAST of MS002399 vs. ExPASy Swiss-Prot
Match: O94654 (Uncharacterized transporter C405.03c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC405.03c PE=3 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.5e-28
Identity = 113/384 (29.43%), Postives = 184/384 (47.92%), Query Frame = 0


Query: 8   GLILLIAVVVIWVSSAEITQSIFRD--YEHPFVMTYIGTSMLVAYLAIAFIKECIVKLFR 67
           G++LL+ VV +W+ S+ +T S+  D  +  PF++TYI T   V YL   +  E   K  R
Sbjct: 12  GVVLLLFVVFLWLISSFLTSSLLDDDNFFSPFLITYINTGTFVFYLIPWYFSE---KKTR 71

Query: 68  THFSNGNSKKVAEVAQPQVMEEKSNNPSSPNNNNNNNISEVGDGSEVHCVVQIQCEEEVV 127
            H      + ++E++  + + + S N  +  N      S +G                  
Sbjct: 72  KH------RLMSELSMYESVHDSSFNLGTRPN------SPLG------------------ 131

Query: 128 SVDKDNYGGCECENQEVAMALKTKETKFTTKQIAVLALTIGPIWFVSEYFTNAALARTSV 187
                                         +Q A L+L    IWF + YF+N++L  T+V
Sbjct: 132 -----------------------------FRQTAYLSLGFCIIWFAANYFSNSSLGFTNV 191

Query: 188 ATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVVVSLAGVAMTTVGKTWAKDQPHSTSS 247
           A+ TI+ S SG FTL +   +  +  T+  ++A++ S+ GV +         D   S  S
Sbjct: 192 ASFTIISSMSGFFTLGLGTIVNVERFTLSKLLALMASVGGVIIVVTQDAKQADLNDSPPS 251

Query: 248 EHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKIDMQKFLGYVGLFTLTTLWWLIW 307
                  +G+A+ALL+AL  G Y V++K +  EE   +  + F G VGLF L  LW  + 
Sbjct: 252 RPA----LGNAYALLAALLYGCYSVMVKFHITEE-SCVSTRLFFGLVGLFDLILLWPFLI 311

Query: 308 PLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSDYFWAMGVVWTSPLVAALGASLTIPL 367
            L   G+E +F++P +T    +++ N  I  F+SDY W + ++ TSPL+  +G SL+IPL
Sbjct: 312 ILHLYGVE-RFSLPSTTAGLIVLIINASI-TFVSDYLWVIAMLMTSPLLVTVGMSLSIPL 326

Query: 368 AMLGDMVLHGRHYSLVYIFGSLQV 390
           A+  D++L G + +   I GSL V
Sbjct: 372 ALFFDILLKGHYLNFSLILGSLLV 326

[Close]

BLAST of MS002399 vs. ExPASy Swiss-Prot
Match: Q04083 (Thiamine-repressible mitochondrial transport protein THI74 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=THI74 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 4.4e-28
Identity = 110/386 (28.50%), Postives = 177/386 (45.85%), Query Frame = 0


Query: 8   GLILLIAVVVIWVSSAEITQSIFRD--YEHPFVMTYIGTSMLVAYLAIAFIKECIVKLFR 67
           G++LL  VVV WV ++ +T  +     Y  PF +TY+  S    YL     +  I++  R
Sbjct: 13  GVLLLAVVVVFWVGASCLTNELLETNAYNKPFFLTYLNISSFALYLTPDLWR--IIQSRR 72

Query: 68  THFSNGNSKKVAEVAQPQVMEEKSNNPSSPNNNNNNNISEVGDGSEVHCVVQIQCEEEVV 127
                   + +    Q    E      S+P  + ++N+S + D                 
Sbjct: 73  KSLQERTERTLPIHTQESFSEFLPLLSSTP--STSSNLSSIAD----------------- 132

Query: 128 SVDKDNYGGCECENQEVAMALKTKETKFTTKQIAVLALTIGPIWFVSEYFTNAALARTSV 187
                                K K+T         L+L    +WFV+    NAAL+ T+V
Sbjct: 133 --------------------TKVKDT-------MRLSLLFCVLWFVANLAANAALSYTTV 192

Query: 188 ATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVVVSLAGVAMTTVGKTWAKDQPHSTSS 247
           A++TIL STS  FTL +   L  ++ +   ++ + VSL G+ +  +  +  +D   ++S 
Sbjct: 193 ASSTILSSTSSFFTLFLATSLGIETFSTKKLLGLFVSLFGIILIVMQSSKQQDSVSASS- 252

Query: 248 EHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKIDMQKFLGYVGLFTLTTLWWLIW 307
                  VG+  ALL +L   +Y  LLK     +G ++D+Q FLGYVG+FT    W ++ 
Sbjct: 253 -----FLVGNTLALLGSLGYSVYTTLLKYEISSKGLRLDIQMFLGYVGIFTFLLFWPILI 312

Query: 308 PLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSDYFWAMGVVWTSPLVAALGASLTIPL 367
            L    +E  F +P +  ++ LV+ NC I  F+SDYFW   ++ TSPLV  +  + TIPL
Sbjct: 313 ILDITHME-TFELPSNFHISFLVMLNCII-IFVSDYFWCKALILTSPLVVTVALTFTIPL 342

Query: 368 AMLGDMVLHGRHYSLVYIFGSLQVSI 392
           AM  D V     ++  YI G + + +
Sbjct: 373 AMFADFVWREAFFTPWYIIGVIFIFV 342

[Close]

BLAST of MS002399 vs. ExPASy Swiss-Prot
Match: Q8WV83 (Solute carrier family 35 member F5 OS=Homo sapiens OX=9606 GN=SLC35F5 PE=1 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 9.7e-28
Identity = 117/410 (28.54%), Postives = 193/410 (47.07%), Query Frame = 0


Query: 8   GLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAIAFI------KECIV 67
           G+++L+ V VIWV+S+E+T  +F  Y  PF  T+  TSM V YL + FI      ++C  
Sbjct: 72  GIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVLYL-LGFIIWKPWRQQCTR 131

Query: 68  KLFRTH---FSNGNSKKVA---------EVAQPQVMEEKSNN-PSSPNNNNNNNISEVGD 127
            L   H   F++      A          +++P  +  K ++ PS    + N +  +   
Sbjct: 132 GLRGKHAAFFADAEGYFAACTTDTTMNSSLSEPLYVPVKFHDLPSEKPESTNIDTEKTPK 191

Query: 128 GSEVHCVVQIQCEEEVVSVDKDNYGGCECENQEVAMALKTKET------KFTTKQIAVLA 187
            S V     ++  +   S         E +   ++  +K +E+      K T  Q+A ++
Sbjct: 192 KSRVRFSNIMEIRQLPSS------HALEAKLSRMSYPVKEQESILKTVGKLTATQVAKIS 251

Query: 188 LTIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDAFLERQS---LTIVNIVAV 247
                +WF++      AL+ T VA   IL STSGLFTLI+ A     S    T+  ++AV
Sbjct: 252 FFFCFVWFLANLSYQEALSDTQVAIVNILSSTSGLFTLILAAVFPSNSGDRFTLSKLLAV 311

Query: 248 VVSLAGVAMTTVGKTWAKDQPHSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEE 307
           ++S+ GV +  +          + S +      VG  ++L  A+   +Y V++K+    E
Sbjct: 312 ILSIGGVVLVNL----------AGSEKPAGRDTVGSIWSLAGAMLYAVYIVMIKRKVDRE 371

Query: 308 GEKIDMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLS 367
            +K+D+  F G+VGLF L  LW   + L   G E  F  P    V   ++ N  IG  LS
Sbjct: 372 -DKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFE-DFEFPNKV-VLMCIIINGLIGTVLS 431

Query: 368 DYFWAMGVVWTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYIFGSLQV 390
           ++ W  G   TS L+  L  SLTIPL+++ DM +    +S ++  G++ V
Sbjct: 432 EFLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQFSWLFFAGAIPV 461

[Close]

BLAST of MS002399 vs. ExPASy TrEMBL
Match: A0A6J1C0M9 (uncharacterized transporter C405.03c-like OS=Momordica charantia OX=3673 GN=LOC111006993 PE=4 SV=1)

HSP 1 Score: 735.3 bits (1897), Expect = 1.3e-208
Identity = 383/390 (98.21%), Postives = 384/390 (98.46%), Query Frame = 0


Query: 1   MAWRYKGGLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAIAFIKECI 60
           MAWRYKGGLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAIAFIKECI
Sbjct: 1   MAWRYKGGLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAIAFIKECI 60

Query: 61  VKLFRTHFSNGNSKKVAEVAQPQVMEEKSNNPSSP-NNNNNNNISEVGDGSEVHCVVQIQ 120
           VKLFRTHFSNGNSKKVAEVAQPQVMEEKSNNPSSP NNNNNNNISEVGDGSEVHCVVQIQ
Sbjct: 61  VKLFRTHFSNGNSKKVAEVAQPQVMEEKSNNPSSPNNNNNNNNISEVGDGSEVHCVVQIQ 120

Query: 121 CEEEVVSVDKDNYGGCECENQEVAMALKTKETKFTTKQIAVLALTIGPIWFVSEYFTNAA 180
           CEEEVVSVDKDNYGGCECENQEVAMALKTKETKF     AVLALTIGPIWFVSEYFTNAA
Sbjct: 121 CEEEVVSVDKDNYGGCECENQEVAMALKTKETKF-----AVLALTIGPIWFVSEYFTNAA 180

Query: 181 LARTSVATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVVVSLAGVAMTTVGKTWAKDQ 240
           LARTSVATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVVVSLAGVAMTT+GKTWAKDQ
Sbjct: 181 LARTSVATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVVVSLAGVAMTTIGKTWAKDQ 240

Query: 241 PHSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKIDMQKFLGYVGLFTLTT 300
           PHSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKIDMQKFLGYVGLFTLTT
Sbjct: 241 PHSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKIDMQKFLGYVGLFTLTT 300

Query: 301 LWWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSDYFWAMGVVWTSPLVAALGA 360
           LWWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSDYFWAMGVVWTSPLVAALGA
Sbjct: 301 LWWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSDYFWAMGVVWTSPLVAALGA 360

Query: 361 SLTIPLAMLGDMVLHGRHYSLVYIFGSLQV 390
           SLTIPLAMLGDMVLHGRHYSLVYIFGSLQV
Sbjct: 361 SLTIPLAMLGDMVLHGRHYSLVYIFGSLQV 385

[Close]

BLAST of MS002399 vs. ExPASy TrEMBL
Match: A0A5D3C4N7 (Putative transporter-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G00180 PE=4 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 4.8e-163
Identity = 309/393 (78.63%), Postives = 342/393 (87.02%), Query Frame = 0


Query: 1   MAWRYKGGLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAIAFIKECI 60
           MAW+YK GLILL+AVVVIWV+SAEITQSIF DYEHPFV+TY+GTSMLVAYLAIAFIKECI
Sbjct: 1   MAWKYKVGLILLVAVVVIWVTSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60

Query: 61  VKLFRTHF-SNGNSKKVAEVAQPQVMEEKSNNPSSPNNNNNNNISEVGDGSEVHCVVQIQ 120
           +K+ R+HF + GN +KVAE+  P    E+  N SS  NNNNN  S+  +  +V  VV I 
Sbjct: 61  MKIVRSHFRTKGNCRKVAEIQSPLPALEERTNQSS--NNNNNTTSDKVENRDVQYVVNIN 120

Query: 121 CEEEVVSVDKDNYGGC---ECENQEVAMALKTKETKFTTKQIAVLALTIGPIWFVSEYFT 180
                 +   +N GGC   E EN E+ + LKT+E KF+TKQIAVLALTIGPIWFVSEYFT
Sbjct: 121 DNNNNSNNSSNNNGGCELREAENMEMRI-LKTEEAKFSTKQIAVLALTIGPIWFVSEYFT 180

Query: 181 NAALARTSVATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVVVSLAGVAMTTVGKTWA 240
           NAALARTSVATTTILFSTSGLFTLI+DA LERQSLTIVN+VAVVVS+AGVAMTTVGKTWA
Sbjct: 181 NAALARTSVATTTILFSTSGLFTLILDACLERQSLTIVNVVAVVVSMAGVAMTTVGKTWA 240

Query: 241 KDQPHSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKIDMQKFLGYVGLFT 300
           KD+P S+S  HGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEK+DMQKFLGYVGLFT
Sbjct: 241 KDEPQSSSYGHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFT 300

Query: 301 LTTLWWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSDYFWAMGVVWTSPLVAA 360
           LTTLWWLIWPLRAIGIEPKF IP+STKVAE+VLANCF+ NF+SDYFWAMGVVWTSPLVAA
Sbjct: 301 LTTLWWLIWPLRAIGIEPKFMIPQSTKVAEVVLANCFVANFVSDYFWAMGVVWTSPLVAA 360

Query: 361 LGASLTIPLAMLGDMVLHGRHYSLVYIFGSLQV 390
           LGASLTIPLAM+GDMVLHGRHYSLVYIFGS+QV
Sbjct: 361 LGASLTIPLAMIGDMVLHGRHYSLVYIFGSIQV 390

[Close]

BLAST of MS002399 vs. ExPASy TrEMBL
Match: A0A6J1EHX2 (uncharacterized transporter C405.03c-like OS=Cucurbita moschata OX=3662 GN=LOC111432715 PE=4 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 8.2e-163
Identity = 302/389 (77.63%), Postives = 341/389 (87.66%), Query Frame = 0


Query: 1   MAWRYKGGLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAIAFIKECI 60
           MAW+YKGGLILL+AVVVIWV+SAEITQSIF DYEHPFVMTY+GTSMLVAYLAIAF++ECI
Sbjct: 1   MAWKYKGGLILLVAVVVIWVASAEITQSIFTDYEHPFVMTYVGTSMLVAYLAIAFVRECI 60

Query: 61  VKLFRTHFSNGNSKKVAEVAQPQVMEEKSNNPSSPNNNNNNNISEVGDGSEVHCVVQIQC 120
           VKLFR+HF NGN K+VAE+      +E+ NN ++ NNNNNNNISEV +G +VHCVV ++C
Sbjct: 61  VKLFRSHFPNGNPKEVAEI------QEQDNNNNNNNNNNNNNISEVENG-DVHCVVNVEC 120

Query: 121 EEEVVSVDKDNYGGCECENQEVAMALKTKETKFTTKQIAVLALTIGPIWFVSEYFTNAAL 180
           EE+       N G CECE +       T+ET F+TKQ+AVLAL IGPIWFVSEYFTNAAL
Sbjct: 121 EEQEKG-SASNNGRCECEAEN-----GTQETSFSTKQMAVLALAIGPIWFVSEYFTNAAL 180

Query: 181 ARTSVATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVVVSLAGVAMTTVGKTWAKDQP 240
           A+TSVATTTILFSTSGLFTLI+DA  ERQSL+IVN+VAV VS+AGVAMTTVGKTWA+D+ 
Sbjct: 181 AKTSVATTTILFSTSGLFTLILDACFERQSLSIVNVVAVFVSMAGVAMTTVGKTWARDEA 240

Query: 241 HSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKIDMQKFLGYVGLFTLTTL 300
            S S  H KHS++GD FALLSA TDGLYYVLLKKYAGEEGEK+DMQKFLGYVGLFTLTTL
Sbjct: 241 QS-SFGHEKHSYIGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTLTTL 300

Query: 301 WWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSDYFWAMGVVWTSPLVAALGAS 360
           WWLIWPLRAIGIEPKF +P+STKVAE+VLANCF+ NF+SDY WAMGVVWTSPLVAALGAS
Sbjct: 301 WWLIWPLRAIGIEPKFGLPQSTKVAEIVLANCFVSNFVSDYLWAMGVVWTSPLVAALGAS 360

Query: 361 LTIPLAMLGDMVLHGRHYSLVYIFGSLQV 390
           LTIPLAM+GDM+LHGRHYSLVYIFGSLQV
Sbjct: 361 LTIPLAMVGDMLLHGRHYSLVYIFGSLQV 375

[Close]

BLAST of MS002399 vs. ExPASy TrEMBL
Match: A0A6J1L0H0 (uncharacterized vacuolar membrane protein YML018C-like OS=Cucurbita maxima OX=3661 GN=LOC111499921 PE=4 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 5.3e-162
Identity = 303/390 (77.69%), Postives = 343/390 (87.95%), Query Frame = 0


Query: 1   MAWRYKGGLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAIAFIKECI 60
           MAW+YKGGLILL+ VVVIWV+SAEITQSIF DYEHPFVMTY+GTSMLVAYLAIAF++EC+
Sbjct: 1   MAWKYKGGLILLVVVVVIWVASAEITQSIFMDYEHPFVMTYVGTSMLVAYLAIAFLRECL 60

Query: 61  VKLFRTHFSNGNSKKVAEVAQPQVMEEKSNNPSSPNNNNNNNISEVGDGSEVHCVVQIQC 120
           VKLFR+HF NGNSK+VAE+ Q Q  +  +NN ++ NNNNNNNISEV +G +V CVV I+C
Sbjct: 61  VKLFRSHFRNGNSKEVAEI-QEQDKDNNNNNINNNNNNNNNNISEVENG-DVLCVVNIEC 120

Query: 121 EE-EVVSVDKDNYGGCECENQEVAMALKTKETKFTTKQIAVLALTIGPIWFVSEYFTNAA 180
           EE E  SV  ++   CE EN        T+ET F+TK+IAVLAL IGPIWFVSEYFTNAA
Sbjct: 121 EEQEKGSVSNNDRCECEAEN-------GTQETSFSTKKIAVLALAIGPIWFVSEYFTNAA 180

Query: 181 LARTSVATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVVVSLAGVAMTTVGKTWAKDQ 240
           LARTSVATTTILFSTSGLFTLI+DA  ERQSL+IVN+VAV VS+AGVAMTT+GKTWA+D+
Sbjct: 181 LARTSVATTTILFSTSGLFTLILDACFERQSLSIVNVVAVFVSMAGVAMTTIGKTWARDE 240

Query: 241 PHSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKIDMQKFLGYVGLFTLTT 300
             S SS H KHS+VGD FALLSA TDGLYYVLLKKYAGEEGEK+DMQKFLGYVGLFTLTT
Sbjct: 241 AQS-SSRHEKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTLTT 300

Query: 301 LWWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSDYFWAMGVVWTSPLVAALGA 360
           LWWLIWPL+AIGIEPKF IP+STKVAE+V+ANCF+ NF+SDY WAMGVVWTSPL+AALGA
Sbjct: 301 LWWLIWPLKAIGIEPKFGIPQSTKVAEIVIANCFVSNFVSDYLWAMGVVWTSPLIAALGA 360

Query: 361 SLTIPLAMLGDMVLHGRHYSLVYIFGSLQV 390
           SLTIPLAM+GDM+LHGRHYSLVYIFGSLQV
Sbjct: 361 SLTIPLAMVGDMLLHGRHYSLVYIFGSLQV 380

[Close]

BLAST of MS002399 vs. ExPASy TrEMBL
Match: A0A5A7U493 (Putative transporter-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00790 PE=4 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 1.2e-161
Identity = 307/392 (78.32%), Postives = 339/392 (86.48%), Query Frame = 0


Query: 1   MAWRYKGGLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAIAFIKECI 60
           MAW+YK GLILL+AVVVIWV+SAEITQSIF DYEHPFV+TY+GTSMLVAYLAIAFIKECI
Sbjct: 1   MAWKYKVGLILLVAVVVIWVTSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60

Query: 61  VKLFRTHF-SNGNSKKVAEVAQPQVMEEKSNNPSSPNNNNNNNISEVGDGSEVHCVVQIQ 120
           +K+ R+HF + GN +KVAE+  P    E+  N SS  NNNNN  S+  +  +V  VV I 
Sbjct: 61  MKIVRSHFRTKGNCRKVAEIQSPLPALEERTNQSS--NNNNNTTSDEVENRDVQYVVNIN 120

Query: 121 CEEEVVSVDKDNYGGCE-CENQEVAM-ALKTKETKFTTKQIAVLALTIGPIWFVSEYFTN 180
                 +   +N GGCE CE + + M  LKT+E KF+TKQIAVLALTIGPIWFVSEYFTN
Sbjct: 121 DNNNNSNNSSNNNGGCELCEAENMEMRILKTEEAKFSTKQIAVLALTIGPIWFVSEYFTN 180

Query: 181 AALARTSVATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVVVSLAGVAMTTVGKTWAK 240
           AALARTSVATTTILFSTSGLFTLI+DA LERQSLTIVN VAVVVS+AGV MTTVGKTWAK
Sbjct: 181 AALARTSVATTTILFSTSGLFTLILDACLERQSLTIVNAVAVVVSMAGVVMTTVGKTWAK 240

Query: 241 DQPHSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKIDMQKFLGYVGLFTL 300
           D+P S+S  HGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEK+DMQKFLGYVGLFTL
Sbjct: 241 DEPQSSSYGHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKVDMQKFLGYVGLFTL 300

Query: 301 TTLWWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSDYFWAMGVVWTSPLVAAL 360
           TTLWWLIWPLRAIGIEPKF IP+STKVAE+VLANCF+ NF+SDYFWAMGVVWTSPLVAAL
Sbjct: 301 TTLWWLIWPLRAIGIEPKFMIPQSTKVAEVVLANCFVANFVSDYFWAMGVVWTSPLVAAL 360

Query: 361 GASLTIPLAMLGDMVLHGRHYSLVYIFGSLQV 390
           GASLTIPLAM+GDMVLH RHYSLVYIFGS+QV
Sbjct: 361 GASLTIPLAMIGDMVLHDRHYSLVYIFGSIQV 390

[Close]

BLAST of MS002399 vs. TAIR 10
Match: AT4G32140.1 (EamA-like transporter family )

HSP 1 Score: 375.2 bits (962), Expect = 6.5e-104
Identity = 204/390 (52.31%), Postives = 270/390 (69.23%), Query Frame = 0


Query: 1   MAWRYKGGLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAIAFIKECI 60
           M WRYK GL L+  VV+IWV+SAE+TQ IF  Y+ PF +TY+G S+++ YL +AF+K+ +
Sbjct: 1   MGWRYKAGLFLIGTVVIIWVTSAEVTQDIFTAYKQPFAVTYLGASLMIVYLPVAFLKDWL 60

Query: 61  VKLFRTHFSNGNSKKVAEVAQPQVMEEKSNNPSSPNNNNNNNISEVG-DGSEVHCVVQIQ 120
            +      S  N         P + ++ S    SP     + I E+G  G+    + +  
Sbjct: 61  CRYLDRRSSKSNK-------IPALTDDSSVELGSP---LRHKIIEMGLQGT----ITKKD 120

Query: 121 CEEEVVSVDKDNYGGCECENQEVAMALKTKETKFTTKQIAVLALTIGPIWFVSEYFTNAA 180
            EE + S ++++      + +E    LK ++ + TTKQIA+  L + PIWFV+EY +NAA
Sbjct: 121 SEEHLSSHEEEDERPLIGKVREEVQTLKQRK-EITTKQIAMYGLYLAPIWFVTEYLSNAA 180

Query: 181 LARTSVATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVVVSLAGVAMTTVGKTWAKDQ 240
           LARTSVA+TT+L STSGLFTL I  FL + +L +  +VAV VS+AGV MTT+GKTWA D+
Sbjct: 181 LARTSVASTTVLSSTSGLFTLFIGVFLGQDTLNLSKVVAVFVSMAGVVMTTLGKTWASDE 240

Query: 241 PHSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEKIDMQKFLGYVGLFTLTT 300
               SS +G+ S +GD F LLSA++ GL+ VLLKK+AGEEGE +D+QK  GY+GLFTL  
Sbjct: 241 SQLNSSLNGERSLMGDLFGLLSAVSYGLFTVLLKKFAGEEGEGVDVQKLFGYIGLFTLVA 300

Query: 301 LWWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSDYFWAMGVVWTSPLVAALGA 360
           LWWL+WPL A+GIEPKF IP S KV E+VLAN FIG+ LSDYFWA+ VVWT+PLVA LG 
Sbjct: 301 LWWLVWPLTALGIEPKFTIPHSVKVDEVVLANGFIGSVLSDYFWALSVVWTTPLVATLGM 360

Query: 361 SLTIPLAMLGDMVLHGRHYSLVYIFGSLQV 390
           SLTIPLAM+ DM++HGRHYS +YI GS QV
Sbjct: 361 SLTIPLAMVADMMIHGRHYSAIYILGSTQV 375

[Close]

BLAST of MS002399 vs. TAIR 10
Match: AT3G07080.1 (EamA-like transporter family )

HSP 1 Score: 112.1 bits (279), Expect = 1.0e-24
Identity = 107/403 (26.55%), Postives = 190/403 (47.15%), Query Frame = 0


Query: 3   WRYKGGLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAI----AFIKE 62
           WR+  GLI L AV  IW++++ + QS+      PF++T+I  S+ V YL +     ++++
Sbjct: 6   WRWVLGLIYLFAVATIWIAASFVVQSVVEAGVSPFLITFICNSLFVVYLPLFEIGRYLED 65

Query: 63  CIVKLF-----RTHFSNGNSKKVAEVAQPQVMEEKSN-NPSSPNNNNNNNISEVGDGSEV 122
               L      R+H       + A +    V   KS+   SS        ISE G+G E 
Sbjct: 66  AYGSLLFWRSKRSHLMELVESEKAVLLGKDVSGVKSDATESSGLVVREQEISEGGNGIE- 125

Query: 123 HCVVQIQCEEEVVSVDKDNYGGCECENQEVAMALKTKETKFTTKQIAVLALTIGPIWFVS 182
             +  I+ E +      D   G   +  +       ++ ++T  ++A ++L I P WF++
Sbjct: 126 SGLENIELEIDASVNVSDGASGVSNKGLD-------EKGRWTRMRVAKVSLVICPFWFLA 185

Query: 183 EYFTNAALARTSVATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVVVSLAGVAMTTVG 242
           +   N +L  T+V + TIL S S LFT ++      +  T + + +V++ ++G  + ++G
Sbjct: 186 QLTFNVSLKYTTVTSNTILSSASSLFTFLVSLIFLGEKFTWLKLFSVLLCMSGTIIVSMG 245

Query: 243 KTWAKDQPHSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGE---KIDMQKFL 302
            +       S S+   K+  +GD  +L+SA    +Y  L++K   ++ E   ++ M +FL
Sbjct: 246 DS------ESKSNAVAKNPLLGDILSLVSAALYAVYITLIRKKLPDDDERNGRVSMAQFL 305

Query: 303 GYVGLFTLTTLWWLIWPLRAIGIEPKFAIPRST-KVAELVLANCFIGNFLSDYFWAMGVV 362
           G++GLF     +  + P   +    +      T K   LV+    + N LSDY WA  V+
Sbjct: 306 GFLGLF---NFFIFLPPALILNFTKRERFNALTLKQFGLVVGKGLLDNVLSDYLWAKAVL 365

Query: 363 WTSPLVAALGASLTIPLAMLGDMVLHGRHYSLVYIFGSLQVSI 392
            T+  VA  G ++ +PLA + D  L G   S     G+  V +
Sbjct: 366 LTTTTVATAGLTIQVPLAAIVDS-LSGNKPSFTDYIGAAAVMV 390

The following BLAST results are available for this feature:

BLAST of MS002399 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134812.1	2.7e-208	98.21	uncharacterized transporter C405.03c-like [Momordica charantia]	[more]
XP_038880237.1	2.4e-169	80.10	uncharacterized vacuolar membrane protein YML018C-like [Benincasa hispida]	[more]
XP_023531720.1	1.5e-163	78.92	uncharacterized transporter C405.03c-like [Cucurbita pepo subsp. pepo]	[more]
TYK06345.1	1.0e-162	78.63	putative transporter-like protein [Cucumis melo var. makuwa]	[more]
XP_022925420.1	1.7e-162	77.63	uncharacterized transporter C405.03c-like [Cucurbita moschata]	[more]

BLAST of MS002399 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q03730	1.7e-35	31.88	Uncharacterized vacuolar membrane protein YML018C OS=Saccharomyces cerevisiae (s...	[more]
A6QL92	3.0e-29	29.31	Solute carrier family 35 member F5 OS=Bos taurus OX=9913 GN=SLC35F5 PE=2 SV=1	[more]
O94654	1.5e-28	29.43	Uncharacterized transporter C405.03c OS=Schizosaccharomyces pombe (strain 972 / ...	[more]
Q04083	4.4e-28	28.50	Thiamine-repressible mitochondrial transport protein THI74 OS=Saccharomyces cere...	[more]
Q8WV83	9.7e-28	28.54	Solute carrier family 35 member F5 OS=Homo sapiens OX=9606 GN=SLC35F5 PE=1 SV=1	[more]

BLAST of MS002399 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C0M9	1.3e-208	98.21	uncharacterized transporter C405.03c-like OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A5D3C4N7	4.8e-163	78.63	Putative transporter-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A6J1EHX2	8.2e-163	77.63	uncharacterized transporter C405.03c-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1L0H0	5.3e-162	77.69	uncharacterized vacuolar membrane protein YML018C-like OS=Cucurbita maxima OX=36...	[more]
A0A5A7U493	1.2e-161	78.32	Putative transporter-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]

BLAST of MS002399 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G32140.1	6.5e-104	52.31	EamA-like transporter family	[more]
AT3G07080.1	1.0e-24	26.55	EamA-like transporter family	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF16913	PUNUT	coord: 147..273 e-value: 3.2E-10 score: 39.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 82..103
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 84..103
None	No IPR available	PANTHER	PTHR23051	SOLUTE CARRIER FAMILY 35, MEMBER F5	coord: 1..389
None	No IPR available	PANTHER	PTHR23051:SF10	THIAMINE-REPRESSIBLE MITOCHONDRIAL TRANSPORT PROTEIN THI74-LIKE ISOFORM X1	coord: 1..389
None	No IPR available	SUPERFAMILY	103481	Multidrug resistance efflux transporter EmrE	coord: 151..233

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS002399.1	MS002399.1	mRNA