MS002367 (gene) Bitter gourd (TR) v1

Overview


Name	MS002367
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Hydroxymethylbilane hydrolyase [cyclizing]
Location	scaffold30: 4433035 .. 4433958 (+)
RNA-Seq Expression	MS002367
Synteny	MS002367

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCACCGGCGCCGGCCCTCCCGGCCCAATCCGCCAAAATCCACTCGGTTCATCTCCTTCTCCCGCCATTCCCGGCCACTCCATTCGGCGTACGGCGGCGTTCACGACGCCTCAGAACTACGCCGGCAGCCTCTCCCGCCTCCTCACTCTCAAAGGCATGGATCCCCTGTGGTGCCCCACCCTCACCGTCCAGCCCACTCCCCACGCCATCAAATCCCACATCCTTCCCCCAAATCTCGAATCCTTCTCCGCCGTCGCTTTCACTTCCCGCACCGGAATTTCAGCGCTCCTCGACGCTGCAACTGAAATCGGCGAACCCTTGCTACCGTCGCAGGGCGAGACTTTTCTAATCGCTGCCCTAGGTAAGGATTCGGAGCTTCTCGACCATGGATTTATTTCCCAAATTTGCCCTAACGCGAGTAGAATTCAAGTCGTGCTCCCTAAAATAGCAACTCCGAGTGGTTTAGTGGAGGCTCTTGGAGTTGGAAACCGCCGGCGGGTTCTGTGTCCGGTTCCTCGCGTCGTGGGGCTGGACGAGCCTCCGGTAGTTCCGAACTTCCTGCGCGACCTGGAGGCGAACGGATGGGTTCCGGTTCGGGTCGATGCGTACGAGACCCGATGGGCCGGACCCGGGTGCGCGAGGCAGCTCGTGGAGAGAGATGAGAAATTGGATGCCATTGTGTTCACAAGTACTGGGGAAGTGGAGGGCCTGCTGAAAAGCTTGAGGGATTTAGGGTTCGAGTGGGAGACGATGAGAAAGAGGTGGCCGGAAATGGTGGTGGCCGCGCACGGGCCGGTGACGGCGGCCGGAGCCGAGAGGCTTGGCGTCAAGGTTGATTTGGTGAGTTGTAGATTCGATAGCTTTAACGGTGTGGTTGATGCTCTTCATTCCAGATGGCAGAGCTTAGAACAGAACCCTAAC

mRNA sequence

ATGAGCACCGGCGCCGGCCCTCCCGGCCCAATCCGCCAAAATCCACTCGGTTCATCTCCTTCTCCCGCCATTCCCGGCCACTCCATTCGGCGTACGGCGGCGTTCACGACGCCTCAGAACTACGCCGGCAGCCTCTCCCGCCTCCTCACTCTCAAAGGCATGGATCCCCTGTGGTGCCCCACCCTCACCGTCCAGCCCACTCCCCACGCCATCAAATCCCACATCCTTCCCCCAAATCTCGAATCCTTCTCCGCCGTCGCTTTCACTTCCCGCACCGGAATTTCAGCGCTCCTCGACGCTGCAACTGAAATCGGCGAACCCTTGCTACCGTCGCAGGGCGAGACTTTTCTAATCGCTGCCCTAGGTAAGGATTCGGAGCTTCTCGACCATGGATTTATTTCCCAAATTTGCCCTAACGCGAGTAGAATTCAAGTCGTGCTCCCTAAAATAGCAACTCCGAGTGGTTTAGTGGAGGCTCTTGGAGTTGGAAACCGCCGGCGGGTTCTGTGTCCGGTTCCTCGCGTCGTGGGGCTGGACGAGCCTCCGGTAGTTCCGAACTTCCTGCGCGACCTGGAGGCGAACGGATGGGTTCCGGTTCGGGTCGATGCGTACGAGACCCGATGGGCCGGACCCGGGTGCGCGAGGCAGCTCGTGGAGAGAGATGAGAAATTGGATGCCATTGTGTTCACAAGTACTGGGGAAGTGGAGGGCCTGCTGAAAAGCTTGAGGGATTTAGGGTTCGAGTGGGAGACGATGAGAAAGAGGTGGCCGGAAATGGTGGTGGCCGCGCACGGGCCGGTGACGGCGGCCGGAGCCGAGAGGCTTGGCGTCAAGGTTGATTTGGTGAGTTGTAGATTCGATAGCTTTAACGGTGTGGTTGATGCTCTTCATTCCAGATGGCAGAGCTTAGAACAGAACCCTAAC

Coding sequence (CDS)

ATGAGCACCGGCGCCGGCCCTCCCGGCCCAATCCGCCAAAATCCACTCGGTTCATCTCCTTCTCCCGCCATTCCCGGCCACTCCATTCGGCGTACGGCGGCGTTCACGACGCCTCAGAACTACGCCGGCAGCCTCTCCCGCCTCCTCACTCTCAAAGGCATGGATCCCCTGTGGTGCCCCACCCTCACCGTCCAGCCCACTCCCCACGCCATCAAATCCCACATCCTTCCCCCAAATCTCGAATCCTTCTCCGCCGTCGCTTTCACTTCCCGCACCGGAATTTCAGCGCTCCTCGACGCTGCAACTGAAATCGGCGAACCCTTGCTACCGTCGCAGGGCGAGACTTTTCTAATCGCTGCCCTAGGTAAGGATTCGGAGCTTCTCGACCATGGATTTATTTCCCAAATTTGCCCTAACGCGAGTAGAATTCAAGTCGTGCTCCCTAAAATAGCAACTCCGAGTGGTTTAGTGGAGGCTCTTGGAGTTGGAAACCGCCGGCGGGTTCTGTGTCCGGTTCCTCGCGTCGTGGGGCTGGACGAGCCTCCGGTAGTTCCGAACTTCCTGCGCGACCTGGAGGCGAACGGATGGGTTCCGGTTCGGGTCGATGCGTACGAGACCCGATGGGCCGGACCCGGGTGCGCGAGGCAGCTCGTGGAGAGAGATGAGAAATTGGATGCCATTGTGTTCACAAGTACTGGGGAAGTGGAGGGCCTGCTGAAAAGCTTGAGGGATTTAGGGTTCGAGTGGGAGACGATGAGAAAGAGGTGGCCGGAAATGGTGGTGGCCGCGCACGGGCCGGTGACGGCGGCCGGAGCCGAGAGGCTTGGCGTCAAGGTTGATTTGGTGAGTTGTAGATTCGATAGCTTTAACGGTGTGGTTGATGCTCTTCATTCCAGATGGCAGAGCTTAGAACAGAACCCTAAC

Protein sequence

MSTGAGPPGPIRQNPLGSSPSPAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWCPTLTVQPTPHAIKSHILPPNLESFSAVAFTSRTGISALLDAATEIGEPLLPSQGETFLIAALGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLDEPPVVPNFLRDLEANGWVPVRVDAYETRWAGPGCARQLVERDEKLDAIVFTSTGEVEGLLKSLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALHSRWQSLEQNPN

Homology

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BLAST of MS002367 vs. NCBI nr
Match: XP_022134809.1 (uncharacterized protein LOC111006990 [Momordica charantia])

HSP 1 Score: 613.6 bits (1581), Expect = 9.2e-172
Identity = 302/308 (98.05%), Postives = 303/308 (98.38%), Query Frame = 0


Query: 1   MSTGAGPPGPIRQNPLGSSPSPAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWCP 60
           MST AG PGPIRQNPLGSSPS AIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWCP
Sbjct: 1   MSTSAGRPGPIRQNPLGSSPSSAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWCP 60

Query: 61  TLTVQPTPHAIKSHILPPNLESFSAVAFTSRTGISALLDAATEIGEPLLPSQGETFLIAA 120
           TLTVQPTPHAIKSHILPPNLESFSAVAFTSR GISALLDAATEIGEPLLPSQGETFLIAA
Sbjct: 61  TLTVQPTPHAIKSHILPPNLESFSAVAFTSRAGISALLDAATEIGEPLLPSQGETFLIAA 120

Query: 121 LGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLDE 180
           LGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGL+E
Sbjct: 121 LGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLEE 180

Query: 181 PPVVPNFLRDLEANGWVPVRVDAYETRWAGPGCARQLVERDEKLDAIVFTSTGEVEGLLK 240
           PPVVPNFL DLEANGWVPVRVDAYETRWAGPGCARQLVERDEKLDAIVFTSTGEVEGLLK
Sbjct: 181 PPVVPNFLHDLEANGWVPVRVDAYETRWAGPGCARQLVERDEKLDAIVFTSTGEVEGLLK 240

Query: 241 SLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALHSRW 300
           SLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALHSRW
Sbjct: 241 SLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALHSRW 300

Query: 301 QSLEQNPN 309
           QSLEQNPN
Sbjct: 301 QSLEQNPN 308

[Close]

BLAST of MS002367 vs. NCBI nr
Match: XP_023003818.1 (uncharacterized protein LOC111497286 [Cucurbita maxima])

HSP 1 Score: 515.8 bits (1327), Expect = 2.6e-142
Identity = 254/309 (82.20%), Postives = 276/309 (89.32%), Query Frame = 0


Query: 1   MSTGAGPPGPIRQNPLGSSPSPAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWCP 60
           MSTGA  P  I  NPL SSPSPAIP +S  RT AFTTPQNYAGSLS LL+LKG DPLWCP
Sbjct: 1   MSTGAAHPSLIHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWCP 60

Query: 61  TLTVQPTPHAIKSHILPPNLESFSAVAFTSRTGISALLDAATEIGEPLLPSQGETFLIAA 120
           T+TV PTP AIKSH+LPPNL  +SAVAFTSR+GI+ALLDAATEI +PLL  QG+TFLIAA
Sbjct: 61  TVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDDPLLSPQGDTFLIAA 120

Query: 121 LGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLDE 180
           LGKDSELLDHG +S+ CPN SRI+VV+PKIATPSGLVEALG+GN RRVLCPVPRVVGL+E
Sbjct: 121 LGKDSELLDHGVLSKFCPNTSRIRVVVPKIATPSGLVEALGIGNHRRVLCPVPRVVGLNE 180

Query: 181 PPVVPNFLRDLEANGWVPVRVDAYETRWAGPGCARQLVER--DEKLDAIVFTSTGEVEGL 240
           PPVVPNFLRDL A+GWVPVRVDAYETRWAGP CAR LV+R  DEKLDAIVFTSTGEVEGL
Sbjct: 181 PPVVPNFLRDLAASGWVPVRVDAYETRWAGPECARMLVKRGEDEKLDAIVFTSTGEVEGL 240

Query: 241 LKSLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALHS 300
           LKSLR LG +WE MRK+WPEMV+AAHGPVTAAGAERLGVK+DLVS +FDSFNGVVDALHS
Sbjct: 241 LKSLRTLGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALHS 300

Query: 301 RWQSLEQNP 308
           RWQSLEQNP
Sbjct: 301 RWQSLEQNP 309

[Close]

BLAST of MS002367 vs. NCBI nr
Match: KAG6587847.1 (hypothetical protein SDJN03_16412, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035661.1 hypothetical protein SDJN02_02459, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 515.4 bits (1326), Expect = 3.4e-142
Identity = 255/310 (82.26%), Postives = 277/310 (89.35%), Query Frame = 0


Query: 1   MSTGAGPPGPIRQ-NPLGSSPSPAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWC 60
           MSTGA  P PI   NPL SSPSPAIP +S  RT AFTTPQNYAGSLS LL+LKG DPLWC
Sbjct: 1   MSTGAAHPSPIHHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60

Query: 61  PTLTVQPTPHAIKSHILPPNLESFSAVAFTSRTGISALLDAATEIGEPLLPSQGETFLIA 120
           PT+TV PTP AIKSH+LPPNL  +SAVAFTSR+GI+ALLDAATEI EPLL  QG+TFLIA
Sbjct: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120

Query: 121 ALGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLD 180
           ALGKDSELLDHG +S+ CPNASRI+VV+PKIA+PSGLVEALG+GN RRVLCPVPRVVGL+
Sbjct: 121 ALGKDSELLDHGVLSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEANGWVPVRVDAYETRWAGPGCARQLVER--DEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDL A+GWVPVRVDAYETRW GP CAR+L ER  DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALH 300
           LLKSLR LG +WE MRK+WPEMV+AAHGPVTAAGAERLGVK+DLVS +FDSFNGVVDALH
Sbjct: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300

Query: 301 SRWQSLEQNP 308
           SRWQSLEQNP
Sbjct: 301 SRWQSLEQNP 310

[Close]

BLAST of MS002367 vs. NCBI nr
Match: XP_023530963.1 (uncharacterized protein LOC111793350 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 513.5 bits (1321), Expect = 1.3e-141
Identity = 253/310 (81.61%), Postives = 277/310 (89.35%), Query Frame = 0


Query: 1   MSTGAGPPGPIRQ-NPLGSSPSPAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWC 60
           MSTGA  P PI   NPL SSPSPAIP +S  RT AFTTPQNYAGSLS LL+LKG DPLWC
Sbjct: 1   MSTGAAHPSPIHHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60

Query: 61  PTLTVQPTPHAIKSHILPPNLESFSAVAFTSRTGISALLDAATEIGEPLLPSQGETFLIA 120
           PT+TV PTP AIKSH++PPNL  +SAVAFTSR+GI+ALLDAATEI EPLL  QG+TFLIA
Sbjct: 61  PTVTVHPTPIAIKSHLVPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120

Query: 121 ALGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLD 180
           ALGKDSELLDHG +S+ CPNA+RI+VV+PKIA+PSGLVEALG+GN RRVLCPVPRVVGL+
Sbjct: 121 ALGKDSELLDHGVLSKFCPNANRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEANGWVPVRVDAYETRWAGPGCARQLVER--DEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDL A+GWVPVRVDAYETRW GP CAR+L ER  DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALH 300
           LLKSLR LG +WE MRK+WPEMV+AAHGPVTAAGAERLGVK+DLVS +FDSFNGVVDALH
Sbjct: 241 LLKSLRALGLQWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300

Query: 301 SRWQSLEQNP 308
           SRWQSLEQNP
Sbjct: 301 SRWQSLEQNP 310

[Close]

BLAST of MS002367 vs. NCBI nr
Match: XP_022926996.1 (uncharacterized protein LOC111433956 [Cucurbita moschata])

HSP 1 Score: 513.5 bits (1321), Expect = 1.3e-141
Identity = 255/310 (82.26%), Postives = 276/310 (89.03%), Query Frame = 0


Query: 1   MSTGAGPPGPIRQ-NPLGSSPSPAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWC 60
           MSTGA  P PIR  NPL SSPSPAI  +S  RT AFTTPQNYAGSLS LL+LKG DPLWC
Sbjct: 1   MSTGAAHPSPIRHLNPLASSPSPAISTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60

Query: 61  PTLTVQPTPHAIKSHILPPNLESFSAVAFTSRTGISALLDAATEIGEPLLPSQGETFLIA 120
           PT+TV PTP AIKSH+LPPNL  +SAVAFTSR+GI+ALLDAATEI EPLL  QG+TFLIA
Sbjct: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120

Query: 121 ALGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLD 180
           ALGKDSELLDHG  S+ CPNASRI+VV+PKIA+PSGLVEALG+GN RRVLCPVPRVVGL+
Sbjct: 121 ALGKDSELLDHGVFSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEANGWVPVRVDAYETRWAGPGCARQLVER--DEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDL A+GWVPVRVDAYETRW GP CAR+L ER  DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALH 300
           LLKSLR LG +WE MRK+WPEMV+AAHGPVTAAGAERLGVK+DLVS +FDSFNGVVDALH
Sbjct: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300

Query: 301 SRWQSLEQNP 308
           SRWQSLEQNP
Sbjct: 301 SRWQSLEQNP 310

[Close]

BLAST of MS002367 vs. ExPASy TrEMBL
Match: A0A6J1BZU3 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Momordica charantia OX=3673 GN=LOC111006990 PE=3 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 4.5e-172
Identity = 302/308 (98.05%), Postives = 303/308 (98.38%), Query Frame = 0


Query: 1   MSTGAGPPGPIRQNPLGSSPSPAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWCP 60
           MST AG PGPIRQNPLGSSPS AIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWCP
Sbjct: 1   MSTSAGRPGPIRQNPLGSSPSSAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWCP 60

Query: 61  TLTVQPTPHAIKSHILPPNLESFSAVAFTSRTGISALLDAATEIGEPLLPSQGETFLIAA 120
           TLTVQPTPHAIKSHILPPNLESFSAVAFTSR GISALLDAATEIGEPLLPSQGETFLIAA
Sbjct: 61  TLTVQPTPHAIKSHILPPNLESFSAVAFTSRAGISALLDAATEIGEPLLPSQGETFLIAA 120

Query: 121 LGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLDE 180
           LGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGL+E
Sbjct: 121 LGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLEE 180

Query: 181 PPVVPNFLRDLEANGWVPVRVDAYETRWAGPGCARQLVERDEKLDAIVFTSTGEVEGLLK 240
           PPVVPNFL DLEANGWVPVRVDAYETRWAGPGCARQLVERDEKLDAIVFTSTGEVEGLLK
Sbjct: 181 PPVVPNFLHDLEANGWVPVRVDAYETRWAGPGCARQLVERDEKLDAIVFTSTGEVEGLLK 240

Query: 241 SLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALHSRW 300
           SLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALHSRW
Sbjct: 241 SLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALHSRW 300

Query: 301 QSLEQNPN 309
           QSLEQNPN
Sbjct: 301 QSLEQNPN 308

[Close]

BLAST of MS002367 vs. ExPASy TrEMBL
Match: A0A6J1KXQ2 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucurbita maxima OX=3661 GN=LOC111497286 PE=3 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 1.3e-142
Identity = 254/309 (82.20%), Postives = 276/309 (89.32%), Query Frame = 0


Query: 1   MSTGAGPPGPIRQNPLGSSPSPAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWCP 60
           MSTGA  P  I  NPL SSPSPAIP +S  RT AFTTPQNYAGSLS LL+LKG DPLWCP
Sbjct: 1   MSTGAAHPSLIHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWCP 60

Query: 61  TLTVQPTPHAIKSHILPPNLESFSAVAFTSRTGISALLDAATEIGEPLLPSQGETFLIAA 120
           T+TV PTP AIKSH+LPPNL  +SAVAFTSR+GI+ALLDAATEI +PLL  QG+TFLIAA
Sbjct: 61  TVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDDPLLSPQGDTFLIAA 120

Query: 121 LGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLDE 180
           LGKDSELLDHG +S+ CPN SRI+VV+PKIATPSGLVEALG+GN RRVLCPVPRVVGL+E
Sbjct: 121 LGKDSELLDHGVLSKFCPNTSRIRVVVPKIATPSGLVEALGIGNHRRVLCPVPRVVGLNE 180

Query: 181 PPVVPNFLRDLEANGWVPVRVDAYETRWAGPGCARQLVER--DEKLDAIVFTSTGEVEGL 240
           PPVVPNFLRDL A+GWVPVRVDAYETRWAGP CAR LV+R  DEKLDAIVFTSTGEVEGL
Sbjct: 181 PPVVPNFLRDLAASGWVPVRVDAYETRWAGPECARMLVKRGEDEKLDAIVFTSTGEVEGL 240

Query: 241 LKSLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALHS 300
           LKSLR LG +WE MRK+WPEMV+AAHGPVTAAGAERLGVK+DLVS +FDSFNGVVDALHS
Sbjct: 241 LKSLRTLGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALHS 300

Query: 301 RWQSLEQNP 308
           RWQSLEQNP
Sbjct: 301 RWQSLEQNP 309

[Close]

BLAST of MS002367 vs. ExPASy TrEMBL
Match: A0A6J1EGG3 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucurbita moschata OX=3662 GN=LOC111433956 PE=3 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 6.3e-142
Identity = 255/310 (82.26%), Postives = 276/310 (89.03%), Query Frame = 0


Query: 1   MSTGAGPPGPIRQ-NPLGSSPSPAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWC 60
           MSTGA  P PIR  NPL SSPSPAI  +S  RT AFTTPQNYAGSLS LL+LKG DPLWC
Sbjct: 1   MSTGAAHPSPIRHLNPLASSPSPAISTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60

Query: 61  PTLTVQPTPHAIKSHILPPNLESFSAVAFTSRTGISALLDAATEIGEPLLPSQGETFLIA 120
           PT+TV PTP AIKSH+LPPNL  +SAVAFTSR+GI+ALLDAATEI EPLL  QG+TFLIA
Sbjct: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120

Query: 121 ALGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLD 180
           ALGKDSELLDHG  S+ CPNASRI+VV+PKIA+PSGLVEALG+GN RRVLCPVPRVVGL+
Sbjct: 121 ALGKDSELLDHGVFSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEANGWVPVRVDAYETRWAGPGCARQLVER--DEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDL A+GWVPVRVDAYETRW GP CAR+L ER  DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALH 300
           LLKSLR LG +WE MRK+WPEMV+AAHGPVTAAGAERLGVK+DLVS +FDSFNGVVDALH
Sbjct: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300

Query: 301 SRWQSLEQNP 308
           SRWQSLEQNP
Sbjct: 301 SRWQSLEQNP 310

[Close]

BLAST of MS002367 vs. ExPASy TrEMBL
Match: A0A5D3BX13 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1369G00630 PE=3 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 1.4e-141
Identity = 256/307 (83.39%), Postives = 276/307 (89.90%), Query Frame = 0


Query: 1   MSTGAG-PPGPIRQNPLGSSPSPAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWC 60
           MSTGA  P GP   +PL SSPSPAIP HS  RT AFTTPQNYAGSLS LL+LKG +PLWC
Sbjct: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60

Query: 61  PTLTVQPTPHAIKSHILPPNLESFSAVAFTSRTGISALLDAATEIGEPLLPSQGETFLIA 120
           PTLTVQPTP AIKSH+LPP L SFSAVAFTSR+GI+ALLDAATEIGEPLLPS G+TFLIA
Sbjct: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120

Query: 121 ALGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLD 180
           ALGKDSELLDH F++ ICPN SRI+VV+P+IATP+GLVEALGVGN RRVLCPVPRVVGL+
Sbjct: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEANGWVPVRVDAYETRWAGPGCARQLVER--DEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLEA GWVPVRVDAYETRWAGP CAR+LVER  DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALH 300
           LLKSL  LG EW+ M+KRWPEMVVAAHGPVTAAGAERLGVKVDLVS +FDSFNGVVD+LH
Sbjct: 241 LLKSLEHLGLEWDIMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300

Query: 301 SRWQSLE 305
            RWQSL+
Sbjct: 301 WRWQSLD 307

[Close]

BLAST of MS002367 vs. ExPASy TrEMBL
Match: A0A5A7U8V3 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00050 PE=3 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 1.4e-141
Identity = 256/307 (83.39%), Postives = 276/307 (89.90%), Query Frame = 0


Query: 1   MSTGAG-PPGPIRQNPLGSSPSPAIPGHSIRRTAAFTTPQNYAGSLSRLLTLKGMDPLWC 60
           MSTGA  P GP   +PL SSPSPAIP HS  RT AFTTPQNYAGSLS LL+LKG +PLWC
Sbjct: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60

Query: 61  PTLTVQPTPHAIKSHILPPNLESFSAVAFTSRTGISALLDAATEIGEPLLPSQGETFLIA 120
           PTLTVQPTP AIKSH+LPP L SFSAVAFTSR+GI+ALLDAATEIGEPLLPS G+TFLIA
Sbjct: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120

Query: 121 ALGKDSELLDHGFISQICPNASRIQVVLPKIATPSGLVEALGVGNRRRVLCPVPRVVGLD 180
           ALGKDSELLDH F++ ICPN SRI+VV+P+IATP+GLVEALGVGN RRVLCPVPRVVGL+
Sbjct: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEANGWVPVRVDAYETRWAGPGCARQLVER--DEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLEA GWVPVRVDAYETRWAGP CAR+LVER  DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRDLGFEWETMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSCRFDSFNGVVDALH 300
           LLKSL  LG EW+ M+KRWPEMVVAAHGPVTAAGAERLGVKVDLVS +FDSFNGVVD+LH
Sbjct: 241 LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300

Query: 301 SRWQSLE 305
            RWQSL+
Sbjct: 301 WRWQSLD 307

The following BLAST results are available for this feature:

BLAST of MS002367 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134809.1	9.2e-172	98.05	uncharacterized protein LOC111006990 [Momordica charantia]	[more]
XP_023003818.1	2.6e-142	82.20	uncharacterized protein LOC111497286 [Cucurbita maxima]	[more]
KAG6587847.1	3.4e-142	82.26	hypothetical protein SDJN03_16412, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023530963.1	1.3e-141	81.61	uncharacterized protein LOC111793350 [Cucurbita pepo subsp. pepo]	[more]
XP_022926996.1	1.3e-141	82.26	uncharacterized protein LOC111433956 [Cucurbita moschata]	[more]

BLAST of MS002367 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS002367 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BZU3	4.5e-172	98.05	Hydroxymethylbilane hydrolyase [cyclizing] OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A6J1KXQ2	1.3e-142	82.20	Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1EGG3	6.3e-142	82.26	Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A5D3BX13	1.4e-141	83.39	Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A5A7U8V3	1.4e-141	83.39	Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=119469...	[more]

BLAST of MS002367 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036108	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamily	GENE3D	3.40.50.10090		coord: 184..302 e-value: 2.4E-13 score: 51.9 coord: 30..160 e-value: 4.4E-5 score: 25.2
IPR036108	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamily	SUPERFAMILY	69618	HemD-like	coord: 33..299
IPR003754	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase	PFAM	PF02602	HEM4	coord: 45..289 e-value: 2.9E-24 score: 85.7
IPR003754	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase	CDD	cd06578	HemD	coord: 36..296 e-value: 3.09411E-27 score: 104.697
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..26
None	No IPR available	PANTHER	PTHR38020	UROPORPHYRINOGEN-III SYNTHASE	coord: 19..302

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS002367.1	MS002367.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0033014	tetrapyrrole biosynthetic process
molecular_function	GO:0004852	uroporphyrinogen-III synthase activity