MS002366 (gene) Bitter gourd (TR) v1

Overview
NameMS002366
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptiondicer-like protein 4
Locationscaffold30: 4411269 .. 4428060 (-)
RNA-Seq ExpressionMS002366
SyntenyMS002366
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTGATGGGGATACTAATTCTATGGCTGCCGATAGCGACAATTTTCAACACACTAGTTTGGGGCCTGTTGAGAGTTTTGTGGGTCTGTCGTTGGTGGATGATCAAGAACCGGGTCCAAGCACAAGAGCATCAGAGAAAGATCCCAGGAGAAAAGCAAGGAAGTAAGTCATTTCATAAGGCTATATTTTTAGTGCATTTGTTTGGCTGTGTTTACAGTCACAATGTCGCATTTAGTTTTTCAACTATCAACAATGTTTTTAATGCTTTCCTAAAGGAAGTACAAATTGATTGTTAAGTTAACGTTCTAAGTGATGAATTTGCTGAGTAGTTGAAATTCATATTTACGCTATCTCATTCTTTAACTTCGTATTTTATGTAACTTTTCTCAAATATACATCCTGTGTGAAACGCGTGTCTTAAGTTCATGCTTTCCCTTGAACTTAATTTTGAGATTTTACAGAACTACTCACATCAACGAAATACTTGTTTCTTTTGAAAGAAGGAGAAGATTTCACTGAACTGCTCAACGTTCTATCACTATCAGCTGTTTTCGTTCCCTTTTTAAATTTCGTACAGATTAGTTTCACTGTTACAAATTGATTATAATATTTTTTCATCTGTCTTTTTGTAGATATCAATTGGAGCTGTGCAAGAAAGCTTTGGAAGAGAATATCATTCTGTATCTTGGGACAGGATGTGGGAAGACACATATCGCTGTACTTCTGATGTACGAGATGAGGCACTTAATCAGGAGGTCCCAGAAGGGTATATGTGTTTTTCTGGCACCTACAGTTGCTTTAGTCCAGCAGGTGAAGGGGCAGTAGCCCCCCAAAACTATTCTCGTATGATAGTTAATTGACGTAAATAAATTCTGTCTGCCGTCTTCTTAAGTTGAAACTTGAAATTATGCAGAAATTTAGTGGTTTTTCTAATGGGAAGAAACTGAGTACATTTCTGTCTTTTCCTTGTTGAAATTTGGCGTAGTGGTAGGGTGAGGCCTTGATCTCTTTCACCTATTGGCATCACCATTTTTCATTGGTTTGTGTTGTAACTTTTAATCAAGGAAGCATGGAAGTGTCATATTTTGCACTTGACATCATTAAAAGAGTAATAAATTTTCATTCACGTGAAAGTTGGCCCTTGGAACCTAATACTACGTGGTAAGCTAACTACCAAATTGCAATTAAGAAATTCAAAACAAGAGTAAAAGTTTGTAACTAACAGAAAATCAGCCAAGTGATAAGACCACGTTTCAAGAACTTTTCAATGAATGACTGCGACAAATGGATTAGGTTCTGTTTTGTCTCTTTATTAAATAGCTGGGAACAACTGAAAATCAAAAGATGGTTTCATGACTGCTTGACTTTCTCACAGAGTAAACGCCAAGTTAGCTTCAACAACAGTTAGACCCACAACGAGCTTAGCTAATAAGTTCTAGCTCCTTGATTATTTCTTATTCAAATAAAGGCATATACTTCTGATTGAGAAAGTGTTGAAGAGAATTCGAGATAATGGACGCTCTTCATTCTTCACCAAAAAACATGAGAAAATATTCCCTGTTGACAATATTTCTCTCAAGTTTTGTTGATAAACTAAGCAATGAGCTATCAGATAACAACCTCTCCCTGTATAGTTTACATTTTATGCCTTTGTCCAGTAGCTCCAAAGATACCATATATGTCAGCCTCTAAGTACTATTTGGTCCACACGTTATCACATGCCATCATTTGTTGTATGTACGTTCTAACATTTTCTATTTGCCATGATCTTCATACCTTTGCTTTTGGTTATCCATAACAAATTATTTTTTTTCTTTTCTTTTTTTTTTTTTTCACCATTGGCACCCAGAATTTCCTGTTCTCCTGTTTGCTGAGTAAAACACATATAATTTTGCTATATCTACAATTATTTTTGACTATGTATACAGTTCATCTTAAAGTTACTTTATGCATACTAATTGCACGCTTAATGTAAAAAATCTATTTGCTTATATAAATGATCTTAGATGAAATATGTCTATCAATATGTGTACGAATGATGCCAAAGTTCAATGTAAAGGATCCTTTCTGTTGCTTGTCACATTTTAGCAAGCAAAGGTTATAGAAGACTCTACAGATTTCAAGGTCAGGGTCTACTGTGGCGGTTCCAAGTGCTTGAATAGCCATTATGATTGGGAAAGAGAGATGGAAGAACATGAGGTGACTATCCGTTTATATTATACCTATTGATATATCTAAGTTAGGATTGCCAAGCGCTAATGTATTATTTTCTGTTGGGAGGGGGCTTTCTCATTGACCTGCCCTTAGGTTTTCTCGTCTTTGCTTTTGGTTTGTTAATATATTTGTTTCTAGTCAGCCATACTTGCATGGTATTTATTCTTGAGATTGGAATATTAAAAAAGAATATGGTTAACTTGTTACATTACAGATATTTTATTATGAACCAAAAAGATTCTATTATGATTTGTTTTAATGTTAGTGTCTTGTGAGAAGATGGTGAAAATAATTAGTTTAGATCGTTTAGTTCAATAGAAATGAATATTTTTCAATGCACACATAAAAAGTTCTCTGTATCTTCTTCTTGATCTCATTTACATCTTATTCCTTGATCTTTTGCAGGTTTTTGTCATGACCCCTGAAATATTACTGCGCAATTTACACCACTGCTATATCAAGATGGAGTTTGTTGAACTTCTAATATTTGATGAATGCCATCATGCTCAAGTCAAAAGTGACCATTCTTATGCAGAAATCATGAGAGTTAAGGATTTCTATATGCATATATGTACTTGGGCCAGAAGGGTTGATATCTCTCCTGCTGACCGCTTAATTTCTTAATATAATTTATGTCCAATATTTATGTAGGTGTTCTATAAAACAAATGTGGCTAAACGACCCCGGATATTTGGCATGACTGCATCTCCAGTTGTAGGAAAAGGTATCTCCTTTTATAATATTTTGAATTTCATAAGATTGTGAGCCTATTCTTCGGTTCTATCAGTTTATTTTTCCTGAGTACACATTCTTTATTTTAATAATTGCAAAGTTTATAAAATAGGTAACACTATGAATCCTTGGAATTTTTTGTCATAAAGATGTCCATGCTTTTTACAGTGTTTTTAAGTACCAGTGCAGGATTCTTGAATTACCGGGTTTTTGTTCATAGAATCAGGCATCTGGTTTCAGCAATTTTTGTTTCTGTAGTGCTTCTATTTCATGTCTATGTCATGATGATTGCAGTTTGTAAATGATAATATTTTCTTCCTTGAATCTACTTTTTCTAATTGGGTTTTTAGTCTCTGAGTTGTTGTCTGGTAAATTTAATTGGTCATATCTTGGTGGCTGGTGGGTCAATTGTGATCCTCAACTAAGCAGGTGGAGTTGTGAGGTTGTTAGGTTACACTTTATGTATTTAGGTTTGCCCACGTGGACTCTTGATCTCGTTCTTTCTGGGATCTACTGATGATAATATTTTTTTGATGAAAGAAGGGAGGTTAATTTTGATCCAACCTGTGTTAAGTGGAATCCTCTATTTTGCTTTCTCGGTTTAAAGTTGGTCGCGTCAGTTGGCTGTATTTTAGGGGAAAAAAAGATTTTTTTTGGGAAGGATGGAGGGAAGGGGTTGCATCTGATTAAGTGGAAAACAGTACTAAGAATGTTGAGGAGGGGGCCCTCCTTTATATAGAGAACCTTATTGATCATTGTGAGTATGAGTGTCATTCCTAAGGTTTGTACTCTGAGAGTGACAAGAATCCTTGGATGTTTTGATTTTCATTTTCTTGGGATATTTTCTACTTTATTGTAGATGACATGTCTTGCCTTTGCTTGCCAGTTTGTTTTATTCAGATCATTGTTCTACTTCTACTAATTCAGGTGCTTGCAATCAGCAAAATTTATCTAAAAGCATCAATAGTCTTGAGAAGTTACTTGATGCAAAGGTGGGTTGTGGAACTACCTGAGGTATATATATATGTATATTTTAATGAGAAACACTGTAGTTATTATTTTTTTTGATAAAGAGACTGTAATTATCATATAGAAAATCATAAAATGCTACAGCTTAATAAATGAATCTTAAAGAATTACAAGGTAAGGTCAGAGGAAAGGCCACTTATGTTAAATATACGAAGGTTCAATCAAGTACTAAACTGGACTCTTTTTTTCTTGAAAAAAATAACAATTCTAACTATTCATAGATCAAGCACAATTCTTCGCTGTTCTGCTTTCATGTGTCAAACCTAAAAACTAATCCCATTGAAGTATTAAAATTTCCTCGAGGAAACACCAAAAATTTGAGCAAACATAGACTTGAAATCACAACTGCCAACCAAACATATTCCAAGAATTTATTTTTATCCCTATGGTCAAGAAAAGTTTAGCTTATAAAATTTGACCGACCTTTTGTAGGTTTATTCAGTTGAAGATAGGGAAGAACTGGATGCATTTGTGTCATCGCCACTTGTTAAAATATATTATTATGGTCCTGTTGCAAATGGGTTATCTGGCTCATTTATGACTTACTGTAGCCTACTCGAGGACATGAAACGCCAGGTATTTTGGCAATTAGTCTTTGTCTTAGCTTTGTTATCTAAATTGACCTCAACTTCTTGCTTTTCTTGCGACAAGAGCAGTGCATAGTGGTTCTTGGTCAGATGAAAGGAGAGCATGAAGATCTGCGGGCCACCAAGAAATTATTATGCCGAATGCATGATAATATCATATTTTGTTTGGAAAACCTTGGTGTTTGGGGGGCATTGCAGGTAAGTTTTTTGGGCATCTAATGCTTTCTCATTTCCCTGTTTTTGTTATTTTCTTTACTTGATGTTTGTGCTGACCTTTTGTTTTGTTTTTTGTTTTTGTGTGGTTGGAGGGCATGGATAAAGAGTTTGAAATGCTCTATCAATAATATAAGGGGTTTTCTTTTTCTTTTTTTATTTTTTAGGTGAGGGTAGCAAATCCTACATTGTTTTAGTAGACAAAAGTTTAGTGAAATTCACATATTTATATGGTTTTTTCTCATTGCACAAAGTACAAAGGATGTATTGTCTCCTTTTCTTTTCATTATGCTGGTGGATGTGCTTCCCAACTCATTTCTAGGGGGATGGAAAGAGACTTAAGTGACCGTTTCTATGTTGGGAGAAAAAAGGTTCACATTATCTATCTTCAGTCATAGATGATAACTATTCTTTTTGGTCTTGGTAGTGAGGAAATGTTTCTTCAGTTTAACGGTATTTGAGTCTTTTTGTTTCAATTCTCGGGAAATTTAAAGTCTAGCGTTTCAATGCTTGGGAAATTTAAAGTCCTCGGGTTTGGATGGCATGACAAATGAGTTTTTTAAAAAAACTTGGAACATCTTGAAACCAAATATAGTAAAGGTGTTCTATTAACAATAGGACAAATGAAACTTACATTTGCTTATATAGTAAAGGTGTTCTATTAACAATAGGACAAATGAAACTTACATTTGCCTCATTCCTAGGAAGATAAGAGCAAACCGAGTAAGTGAGTATAGAACTATTAGCTTGATCACTTACCTGTATAAAATAATTTCCAAGGTGCTGGCTGACAGACTAAAAAAAATCCTTCCTTTTATCATTGATGATGCTCAAGCGGCTTTTGTAAAGGGGAGACAGATTATTGACGCAATCATGGTGGCTTCGGAGGTTGTGGGGGATTGGAAAGAGAAGAAAAAGGAAGGTTTTTTTTTTTTTTTTTCCTATCAAACTAGATCTTGAAAAAAGCCCATGAAAAGGTGGATTGGGGTTACCTAGATGCCATTATGGAGTTCAAAGGCTTTGGCCAAAGGTGGAGGATGTGGCTTAAAGGCTGTCTTTCTTCGGTCAATTTCTCCGTTCTTATAAATGGTGAACCGAGAGGGAAGATTTTTGCAAGAAGGGGGATTGACAAGGAGATCCACTCCCCCCTTTCCTTTTCACTATTATCGATGATTCCTTCAGTCTTCTGTTGTGAAAAAAGTTCTCTAAGAAGCTCTTCCCAATTCACGTACCAACGATTACTTCAACGCAGAACGAAATAAATGCACTTCGAAAACGTCGTCGGCAATTACACCGGTTTAGTGCTGCACTTCGAAAACGGCGTCAGCAATTACACCGATTTAATGCTCACCAACGGATTTCACCGGATATTAAGTGACGCTTCGTCTTATATATATCACAGATTTTATCATTTGTAATATATAAACTTTGTCTTATATATGCTTTGTCTATATATCTTCCTTCATCTTCTTCTACTGTTTGTCGGAAATACATATACAAAGTGTCCGGGGTACCAGTGGAGTGAGAGTACGCACAGCGTCTACGGAAGGGGGAAGTCCACATCGAGATCAAATGTGGGTTGAGTTGTAGGAGGAGAAGAGAAGCCATCGAAAAACAAGAAGTATGTACATCGAACTAGAACATACATGTAATACATTCGAAAAGATGGCAAGTAAAGCACACGCACGGGCAGATAGATTGGCGGAAGAGAACAAACAACTCCAAGATAGAGTGGCAGCATTGGAAACTTGTTTGCGCGCTCTGGAGGAACTGTGGGGATAGCTATATGTTTATTTGGGTAACACGTTCGGGTCAGACACGTGTCAGACACTCCAATACTTTTGACACTTATTGGACATGTATCGCGTACTTGTTAGTTCAATAGATGTGTGTCACACACTAGTCGTACAAAGTGAATATAGGATCGACACTTGTGAGATACGTATTGAACTCTTGTTAAGCATCAGAAATAGAGGTAAATAGTCATATATGATAAAATAATAAAATTTGAGAGCAAAATACCTCCAACTCATTTTTTAAGAATAAATTGCATAAATTTATTGACTTTGAATGGTATAAAAATGATATATACTTAAAAAAAATTGTATATTTTAATAAATGTGTCATTGCAGTGCCGTATCCTAGATGTTTAGGAGATAACGTGTCACCGTTGTCCGTGTCGTATCTTTGTCTTGTGACCGTATTCGTGCTTTTTAGATACTCATTATGAAGAGTTACCAAGAGGCATCATCTTTTAAAATCTAACATAATGGTGGGTGATCCTGTGTGGAAAAATCATGTAGATCTTTTTCTGTTTGTTTACTATTGTCTGTCTTCATGTTGGCTTTACTTATTTTCTGTTTTATTATCTGTCTTATTAGGCTAGTAAAATTCTTCTAAGTGGAGATAACTCTGAGCGAAGTGAATTGATAGAGGCAGCAGAGGGAAACCCAAATAATCACTCCTTAAGTGATAGATACCTTAATCAAGCTGCTGAAATTCTTGCCAAAGGTTGCAAAATAGGTACTTAAAATTTCTTCTGGAACAGATTGGATTTGCATTCCTCTCTGATTTAAGGCTGCTATGCTCATTCTTGTGATCTTGTGCTCAATTCTACAATTCAGGCATTATGTACAGTTCTTCATTGCTGGACCAGTACTGTCAACAACTGTAGTATCATGATAACCTTTGTGCTCCTTTAACTGACATATTTGTGGTTTATTTTTCTTTTTTCCCGTGCTGTAGGTGGTGGAATGTCAGATACTTTAAATGCTGATATTTTGGAGTATCCATTTTTCTCAAAGAAGCTTTTACAACTTATTGAAATTCTTTCTTCCTTCAGGTTAGAATTGAAATCTTTTACCATCTATTATCTATTATTATATGTTAAAAAGGGATTTTGTCTGTGAAGAAATTTGTATGCTGTAATATTATTGAAATTACACAACCTAAAACGACGTACATGTATACACAAATCTATGGGGTGGCTAGATCTTCTTAGCAGGTCCGCACGCTTATGTCATTGGTAAATATAAATTTGTATTCAAGAAAGTGGATTTCTTGGAAAGCTTGCTAAGACCTGCTATTTTCTCTTTGTTTCTTCATTTCAGGCATCGACTCAACATGAAATGTATTATTTTTGTAAATAGAATAGTTATAGCACGGTCCTTGTCATACATATTGCAGAACCTCGAGTTTCTGGCATTTTGGAAGTGTGATTATCTTGTAGGAGTCCACTCTAAATTGAGGAGCATGTCAAGGAAGACCATGAACCATATTCTTATGAAGTTCCGGTCAGGGGAGGTAAGTTGTTACATCAAACCCCCCACCCAAAACCCCCACACACACACCAAGAGGCTAGCAAATGTGACCAGGTATCTCCTTGTTAGATTATTTGGTTCTGCTCGGCATTTCCTGCATTATGTCTTTGAAGGGAGGAGAGTTTGATTGAACTTCAAGTCATCAATTCCGTTGCTAGAACATGTCCTTCAGTTACTGTGTAATGTGTAATATGAATGTCCCATGGTCCATTACAAATATTTCTTTTGTTTTCGCTCCTATTAAAATATTTTTATTAACAATAAAAGGATGTAGCAGGTCTGAAGAGGATAAAGTGGAGGCAAAGAACTCAAATATCCTCCCAAACTTAAAACCCCCTGAAGGGTAAAGTATTACTCAAATATCTAGAGTAATTACAAGAACTCTTCAATGAAGAACTCCGAGTGAAAGATAGAAAATACACTAGGTCCAATAATCATACATATTCCTTTCAATCACTTCAAAACTTTTGCTAATTCCTCCTAACCACATTTTCTGAAAAAGTGCCTTCGATACATTACCTACACAATTCTAGTCCCTTTTTATGAAGGTATAATCCTGGCTACCGCTTTATTACACTAATGATATTATTATTCCTATATTTTCTTACAATGTTCGATCGTGACACGTCATGGTTCAATCTTTTAGAATGGTTCCTTTGGAAAGATGGCCTATGGACCAGGACCAGCCTACCTTTACTTTCTGCAAGGATATTTTCTTCACTACAAGCATTAGTATATTGTGCTGGAAAATTTCCCTGTGTATCCATAAATAGAGGAGTAATGATGACATTAATTTGTAAAAGAGGGCGAATCCCCATCATCTACTAATAGCATGAAGAAGTAAATTAATCTTATACTGGCTCCTTAAGGGTTGCAGCCAATATTTGTATTTTCATCCTTCTTAGCTATCAAACTACTCCTAATCCCAAAACGCTGAGTTGTTAGGTTAGGGTAATATTAGTTATTTAATCCTCACAAATGAGCTCTGACATGTAGACTAAAAAATACCATAATGCGTGTTTCTTGTCAGTAAGATAAATAAATCAAATAGCATTGATACTGATTGAATTTTTAACTTGACGTCTCCTATCCCAGCATCATGTTAAACTATCATCAATCTTACAGGCTCAAACTATTAGATAAAATCATTCTCACTTATTCTCAATATTAATGAAAGTCTTGTTTCTGATTATTTTAACCTATTCTAGTATGTATGAGGCTAATGATTGTTTTGCACTTCAAATAATTGAAATTTATGATAAATCCTTGTGGCTCATTGCTCTCTGGCAGCTAAACCTCTTGGTTGCGACAAAGGTGGGTGAAGAAGGGCTTGATATTCAGACTTGCTGCCTCGTGATACGATTTGATCTTCCCGAAACAGTATCTAGCTTTATACAATCAAGAGGTCGAGCACGAATGCCACAGTCTGAATATGCATTCCTTGTTGATAGGTCCTCTTTTTATATTTATTTAAATCACAAACATTTTTCTAATAATATTCTTTAGCTGAACAAATTCTTCCATATGTCAGTGGAAATGAAAAGGAGCTAGGATTGATAGACGAATTTCAGAAAGATGAGAATCGGATGGACAGGGAAATTTCTTCTCGCTCATCCAGTGAGATATTTAATAGTCATGAAGAAAGCATCTATAGAGTTGCTTCAACTGGTGCATCTATCACTTCTGGACGTAGTATATCGTTACTGCATGAATTTTGCTCAAAACTTCCACATGATGAGTAAGCCTGAACGTTAAAGCTTTCTCATTAATACAGGATTACATATTCAATTACTTAAAACTCGTCCATTGTGCTTAATTTATCTTTAATACTATATAAATTTTCAAGATATTCTTGTATGAACAGGTACTTTGATCCCAAGCCAAAGTTTTTCTATTATGATGATCTAGCAGGAACTGTATGTCATGCTATTCTGCCCTCCAATGCTCCAATACACCAAATTATCAGCAGAACACAACCTTCTAGGGAAGGTGCTAAAAAGGATGCTTGTATAAAAGCTATTGAGGAATTGCATAAACTTGGTGCTCTCAATGACTTTCTTTTGCTAATGAGAGACGACGCCAATGAAGAAGAATTGGAACTTGACTCCTCTGATTCTGACAGATCTGAAGGTTCACTTGAGTAATTACCTGATTTTTAATTTTGATCTTTATGGGTCTTGATTGATATTTTAAGCATCATTGCAGATGAGACTTCACGAGGAGAACTTCATGAGATGTTAGTTCCTGCTGCTCTAAAAGATTCTTGGACTGGCTCGGGATATTTTATTCTCAACTGTTACCAAATTAAATTCAGTCCACATCCTAGAGATAGGATCTATAAAGAATTTGGTTTATTTGTCAAGGCACCACTTCCCCAGGAGGCTGAGAGAATGGACATTGAGCTTCATCTTGCACGTGGTAGATCTGTAATGGTAAATTTGGTGCCATCAGGGGTTGTGGAGCTTTTGGAGGATGAGGTAAATGGTATATCTGTTTGCTTTGTAGTTTGTACTAAATAGTAATCTTTATCTTTTTTTAACTTACTCAATGTATCTATGCTTTTCAGATTACCCAGGCAGAGAGTTTTCAGGAAATGTTTCTTAAGGTCATTTTGGACAGATCAGAATTTGTTCAGGAGTTTATTCCTTTGAGGAATAATGTCTCTAAATCTATTTCATCAACATCTTACTTGTTGCTTCCTCTGATTTTTCATGATAACGAGAGATCAGTTTCTATAGATTGGAAAGTTATTAGAAGATGTTTATTCTCCCCAATTTTCCAGATTCCTGGCTCTATTGTCAAGGAAACTGCTTGCTCGCATGCCCACTTGACTCTTTATGATGGCCGTAGAAGGACAAGTGATATCAAGAATAGTTTAGTGTATGTCCCGTACAAGCGAGAATTCTTTTTTGTTACTGACATTGTCAAAGGGAAGAATGGATACAGTCGAAGTCACTATAAGAACTCAAGCTCTTTAAGTCATTTTGAGCATTTAATTGAAAGGTGGGTTTCATGTTTTCTGGATTAGAATTTATTTAATGCTTCTCTAGTCTATGCAGAGCCTTTTAGAAAGACTTTTATTTTTCTGGTGTACATTTGGTTATATATATATGAGATAACCACAGTCAAGATAGAGCATAGTTCAGCGGTAATTGACATGTACTCTTTCCCTTGAGGACAAGGTTGGAGGTTCAATCCCCCATATATACAAGATAACCACATGAAGATATCAATTTGGCAATGAATTAGAATTTGACTCTAATATACTCTAGTATTCTTATATCTTTCTTTGTTGTTATTTGTATAACATTTATAAATTTGTATGAATGTTGTGATCAGGTTTGGCATTCATCTTAAATATCCTGAGCAGCCTCTTCTTTCTGCAAAACCCCTATTCCATTTGCACAACTTGCTACATGATAGACGGCAGGGCAATTCTGGTAATATTCTTAGTTCTTTTGCTACTTAGCCTGGTAATTGTAGTTTAAGTTTGCTGCATCAAATGTCTGGATATAGTACTTAAAACAGTCGGATCAAGTTTTCATTTCTGTAATTATTTTGACATCTTTTGCAACATTGCTCGAAACTCATTATTAAAGATTTTTTAAAATTTTTCTGTTAACTTAAATGATATTTTTGGTACTTGGCCTGAAGGTATCGGGTCACTTTGTTGCTTGATTATATTAACTGAATTTTTTTCTTGCTGAAAGAATCACCATAGATAAAGACAGAGAGTTTCTGTTGATCTCAACAATTAGAACAGATCCATAAAGTTCGTTGAAAAGAAATTTCAAAGTATAGTCCCTTCCTGGATCAAGAATCTTGTGTCTCTCCCTGACCAGAGTAATCAAGTAAAAGCCCCTTCCCCCTTCCCTTCCTTCTTATAGTTATCTTCCCCTGGACCAATCACAATGCATTTGTCTGATGGGATCTGACAACTCGCAACAAAGAAAGTAATACTACCCCTACTAACGGACAAATCTGCTTCGGGAATCGTATATTTTTCTCAAATTACTCTCACTTATTGGCTATCTAATGATTGTCTTCCATGGCCTCTCTTCCCTGCCTTTTTTGTCGGAATACATGAAATGTATGGGTGGACCATCAGTTGCCTGTGTGATAACAGTTCAATTACAACGTTTAAGTTTCCTTAATACTTGCAAACTAAATTAAACTAACCACATCTTTTAAATGGTATGCTAATTATCATTCTTTCCAACTGTTAATTGTATTGTGATCAAAGTAATAACATATGTTTCTTAGATATTTAAATTGAGGAAGCCTAACTATTTACCATCCCCCTTCTAATCAGAAAGGCTCGTCTCTTTTTCTTTTAATAAGACTTAGAGGAAAGGTTTACCAATGATGACAATTAAATGCCCTTGCTTGATTTTGAAACTGTCTTAACCTTTCGGCATTTGCATTTGTTTTTCCTTAAAGAATTTACAAAATCTATATCTTGATACAGAAGCATGTTATCTGCAAGAGTATTTCATTGATTTGCCTCCCGAGCTTTGTCAGTTGAAGATAATTGGCTTTTCAAAAGATATCGGGAGCTCCATATCTTTGTTGCCATCAATTATGCATCGTCTAGAAAACTTGCTTGTTGCTATTGAATTGAAGTGTATGTTAGCAGCTGCTTTTCCAGATGGAGCCGAGGTTACTGCCAATAGAGTAAGTGCTTTGTCTTGCAGTTTGAAATTTCTGTTCACATTTGGTTCTTTTCATGCCTTGAACAGTTTTGTGTTTTCCTATTAGATTCTTGAAGCACTCACCACTGAGAAGTGCCAGGAACGCATTTCCCTGGAAAGGCTTGAAATACTTGGTGACGGTTTCCTTAAATTTGCAGTAGCAAGATACCTTTTTCTTACACACGACAAGTCTGATGAAGGAGAACTTACTAGAAGGCGTTCTTATTTAGTGAAAAATATCAATTTGTTCAAGCTAGCAACTAGAAAGAACTTACAGGTATGATGCAAATCCAAGGCCGTGATCTTAAAGTCCTAGGTGCTAATTTGAACCACCATTTCACTGTTTATGCTTTTTCTGGAAAAAAAGAAAAAAAGAAGGAAACTTAAAAGTAAATTGTTGTTCTATTGTTAATAATTTGATCACTTTTCTATTGTACTCTTCAGTATAAGCTTGTTAGTAATTGTTCAACCATGCATCCTTGAGTTGAAGAATCAGGAATTTCCATAGTCGATAAGTAAATCTGATTTTTAATCACTTTACTATTCCTTTACAATGATTAGTGATGACATCGGTGTGATGGAAGTTATCTGTACATTATATATTTGGGAATCTTTGATGATTGGTCTTCAGTTTCAAGATTTGATTTTATCGAAGAAAGAAAGTCCTCCTCGGTTGCTGTAATATTATTGAACAAATCCCAATGTTGTTATAAACTATTCTTAATGATTGGTGCTTCAAGTCACATAAAGAATTGACAACATCCTCCTCGATTGCTCATGTTGTTAGAACAAATATCAATGACCATGAGCAGTTCTTGTTATAAACTATTCTTAATGATTGGTGCTTCAAGTTACATAAAGACATTGTGTACATATCCTGAGATTGATCAATAAATGCTGTTGGCACTTATTAGTCTGTTAATTTCGAATAATCTTAGTGCATGTATGTGCTCTTTCTTCAGGTATACATACGTGACCAGCCATTTGAGCCTAGTCAATTTTATACACTTGGTCGTCCTTGCCCAAGAATATGTAATGAGGAAACTAGAGAAGATATTCATTCGCACTGTAATGCAACAAAAAATGCAAAAGCCAGTGAGACCAAGTGCAGTAGAGGCCATCATTGGTTACATAAGAAAACAATCAGCGACGTGGTTGAAGCTCTGGTTGGGGCGTTCCTAGTTGACAGTGGCTTCAAAGCTGCAATTTCATTTCTAAAGTGGATCGGCATACAAGTAGAATTTGAAGCTTCACTAGTTACCAATGCTCTCATGTCAAGCAATGCTTACATCATACTTGCAGATAGAATAAACATTTCTGTACTTCAAAACTCATTAGGCCATCAATTTCTCCATAAAGGCCTGCTGCTGCAGGCACTCGTACATCCTTCTTACCACAAGCATGGAGGAGGTTGTTACCAGGTACGAACTTTCTCTTTATCCATCTCAAATGGTACTCTGTAATCTGCATTTCTACTGCGTTTGGAGATGGTATTTTCTCTTTATCAATTTCTCCATAAATGGTACTCTACATATTGATGATTTTGCTATGGTTTTGACCAGAGATTAGAGTTTCTTGGAGATGCTGTCCTGGATTACTTGATCACGTCATATCTATATTCAGCATATCCTAAGCTGAAGCCGGGTCTATTGACCGATTTGAGGTCAGTGTTTGTGAGGAATGAGGCCTTTGCCAATGTTGCAGTAGATAGATCTTTCTACAAGTATCTGATTTGTGATTCTACTAGCCTTCAAAGTGATATAAAAAGTTACGTAAACTTCATCAAAGCACCTTCTTCTGGAAGTGGTTCACTTGGGCTTCCGAGTTGTCCAAAGGTACTAGATACGCATTGATACATACTTTTAAACAATTCTAGAATTATCACGTGGACATTTCTAGTGACCAGGAGTATCTGTCAATTTAGTTGGAAAACACCTCTAAATTATACTTTTCAAGTAAATTCCAGTCTCTATAGATAGTATATAATTTCACTGTAGTTTCATACCAGTTGCATCTTTCAGTGACCCTCTTGCCCTCTATGCAAGAGAAGTTTTCTTCTCTGAGTTATTTATTTATTTTATTTTACTTACAGGCAATAGAAATTTCATTTACTCGAGATGTAAAAGGACCAATGTTTATAAATTGTTCTTGTCTTTATATATATTAGTCCTGGGGAAAAGATATTACATGCTGTACTGTCTTTGTATTAGTTGAGAGTATTTAAGGTTTAGGGAATGATTTTGTCGTGTTCGAGTGTCAAGAGAGGGAAGTGTGTTCTTCTGTTTGTTTGAAGATTTCAATGCTTGTATCTTATGTTGCAGGCTTTAGGAGATTTGGTTGAATCTTGTGTTGGGGCCGTTCTTGTTGATACAGGGTTCGACATGAACTATGTTTGGAAGATAATGCTATCTTTTCTGGATCCTATTATAAGCTTTTCAGGCTTCCAGCTTAGTCCTATTCGAGACATAATAGAATTTTGTCAAGATTGTGGCTCGAACCTTCAGTTTAATGAATCAAAAACAGGAAAATATTATTCTGTTGAAGCAGAGGTAAAAGGAGATAACTTTCATGCCACTGCTTCTGCAGCCCATCGGCGAAAGAGAGATGCCAAGAAAATTGCTGCCAACCTCATACTCACCAAGTTAAAGGTGAATATGAGTATAATCATACTTCTACAGTGCTCTTATGCCAAAAAACCATCAGCATTCTTTTACTTTACAATAAACAGACATTGAATATCATTGGAGTTTTTAATATTTGTTTTACACTTCACATTTTTGGAAAAGTCTCGTGTTAATGCTCAAAACAGTTGATATTTCTGGTCTTAAAGGTCCCTAAATATCTTGCTCTGTTCATAACTTTTAGAATAAAGATTTCTGGGAGGTGGAATTCTTTGGTAACCTCAAAAGAAAAAGAACAATATCATCACGGAGCTTAGTTCAGATGTTTTATATATGTTGTAAATTTACTATTTTGTCTAGTTTGGCCACCTGCTCTGTAGTATACTGAGTTGGATGGATCAAGTATCTATAGTTGTCTTTCGGTGCAAAGAATCTTTCAACTTTGAAGTATAGAGCTTTTGGGAATGCTAAAATTTTTAAATTTTGATCTGTGGTATCATCAAGTATTTCGATTATTTGTGACTTTAGACATGCCTCTGTTTCCCATGAACCATGACTTGCTAAGCTTATTTTTACCTACTTTTAAATGGTTTTAGTAACTTTATTAACTTATTGCTGCAGGCCCAGGGACTCATACCAGAGGTCAATTCATTGGAGGAAATTTTAAAGTCAAGCAGGAAGATGGAACCTAAATTGATTGGATATGATGAAACACCCTCTGTCACGATCGATCAAGCTGATAATGGTTTTGGAACAATAAAGGGGTTGGAATCTTCCGGTGCAGACTTAGATCCTTTTGTGCACAGTGAAGTTGGCAATGCTCATCCAGCACGCATTACACGGATAAGCAAAATGCTCGTATCTTCATCTGGAGCTGCACAAGAACAACTGGATCCTAGTCTGGCCTTTGATGGTCATGATAGTCCTACAGACGTGCAGTCGTCATCAGGTACCGCGACTGATAACTGTTCTTGGAGGGGGGTTATTATATGTCAAACTATTTTCTAATTAAAAGCTGTTTCTTTAGGTCGGTTGTGCAAAGCAACAGCCAGATCACGTCTGTATGAAATCTGTGCTGCAAACCATTGGAGTCCCCCTTCATTTGATTGCATGAATGAGGAAGGACCAAGCCATTTGAAGATGTAAGTCCTTGATGAATGCTGAGCTATCTGGGATAGGCATAGGAGAAATGGAAAAGGCTGGTTTTTGATTCAATATTTTTGCAGGTTCACTTACAAGGTTGTTTTAGAGATAGAAGAAGCCCCCAATACGATTTTCAAATTCTTCGGAGCACCTCATTTGAGGAAGAAGGCTGCGGCTGAGCATGCAGCCGAAGCAGCTATTTGGTACTTGAAAAAGGCTGGGTACTTGCCTGAGACAGAT

mRNA sequence

ATGCCTGATGGGGATACTAATTCTATGGCTGCCGATAGCGACAATTTTCAACACACTAGTTTGGGGCCTGTTGAGAGTTTTGTGGGTCTGTCGTTGGTGGATGATCAAGAACCGGGTCCAAGCACAAGAGCATCAGAGAAAGATCCCAGGAGAAAAGCAAGGAAATATCAATTGGAGCTGTGCAAGAAAGCTTTGGAAGAGAATATCATTCTGTATCTTGGGACAGGATGTGGGAAGACACATATCGCTGTACTTCTGATGTACGAGATGAGGCACTTAATCAGGAGGTCCCAGAAGGGTATATGTGTTTTTCTGGCACCTACAGTTGCTTTAGTCCAGCAGCAAGCAAAGGTTATAGAAGACTCTACAGATTTCAAGGTCAGGGTCTACTGTGGCGGTTCCAAGTGCTTGAATAGCCATTATGATTGGGAAAGAGAGATGGAAGAACATGAGGTTTTTGTCATGACCCCTGAAATATTACTGCGCAATTTACACCACTGCTATATCAAGATGGAGTTTGTTGAACTTCTAATATTTGATGAATGCCATCATGCTCAAGTCAAAAGTGACCATTCTTATGCAGAAATCATGAGAGTGTTCTATAAAACAAATGTGGCTAAACGACCCCGGATATTTGGCATGACTGCATCTCCAGTTGTAGGAAAAGATCATTGTTCTACTTCTACTAATTCAGGTGCTTGCAATCAGCAAAATTTATCTAAAAGCATCAATAGTCTTGAGAAGTTACTTGATGCAAAGGTTTATTCAGTTGAAGATAGGGAAGAACTGGATGCATTTGTGTCATCGCCACTTGTTAAAATATATTATTATGGTCCTGTTGCAAATGGGTTATCTGGCTCATTTATGACTTACTGTAGCCTACTCGAGGACATGAAACGCCAGTGCATAGTGGTTCTTGGTCAGATGAAAGGAGAGCATGAAGATCTGCGGGCCACCAAGAAATTATTATGCCGAATGCATGATAATATCATATTTTGTTTGGAAAACCTTGGTGTTTGGGGGGCATTGCAGGCTAGTAAAATTCTTCTAAGTGGAGATAACTCTGAGCGAAGTGAATTGATAGAGGCAGCAGAGGGAAACCCAAATAATCACTCCTTAAGTGATAGATACCTTAATCAAGCTGCTGAAATTCTTGCCAAAGGTTGCAAAATAGGTGGTGGAATGTCAGATACTTTAAATGCTGATATTTTGGAGTATCCATTTTTCTCAAAGAAGCTTTTACAACTTATTGAAATTCTTTCTTCCTTCAGGCATCGACTCAACATGAAATGTATTATTTTTGTAAATAGAATAGTTATAGCACGGTCCTTGTCATACATATTGCAGAACCTCGAGTTTCTGGCATTTTGGAAGTGTGATTATCTTGTAGGAGTCCACTCTAAATTGAGGAGCATGTCAAGGAAGACCATGAACCATATTCTTATGAAGTTCCGGTCAGGGGAGCTAAACCTCTTGGTTGCGACAAAGGTGGGTGAAGAAGGGCTTGATATTCAGACTTGCTGCCTCGTGATACGATTTGATCTTCCCGAAACAGTATCTAGCTTTATACAATCAAGAGGTCGAGCACGAATGCCACAGTCTGAATATGCATTCCTTGTTGATAGTGGAAATGAAAAGGAGCTAGGATTGATAGACGAATTTCAGAAAGATGAGAATCGGATGGACAGGGAAATTTCTTCTCGCTCATCCAGTGAGATATTTAATAGTCATGAAGAAAGCATCTATAGAGTTGCTTCAACTGGTGCATCTATCACTTCTGGACGTAGTATATCGTTACTGCATGAATTTTGCTCAAAACTTCCACATGATGAGTACTTTGATCCCAAGCCAAAGTTTTTCTATTATGATGATCTAGCAGGAACTGTATGTCATGCTATTCTGCCCTCCAATGCTCCAATACACCAAATTATCAGCAGAACACAACCTTCTAGGGAAGGTGCTAAAAAGGATGCTTGTATAAAAGCTATTGAGGAATTGCATAAACTTGGTGCTCTCAATGACTTTCTTTTGCTAATGAGAGACGACGCCAATGAAGAAGAATTGGAACTTGACTCCTCTGATTCTGACAGATCTGAAGATGAGACTTCACGAGGAGAACTTCATGAGATGTTAGTTCCTGCTGCTCTAAAAGATTCTTGGACTGGCTCGGGATATTTTATTCTCAACTGTTACCAAATTAAATTCAGTCCACATCCTAGAGATAGGATCTATAAAGAATTTGGTTTATTTGTCAAGGCACCACTTCCCCAGGAGGCTGAGAGAATGGACATTGAGCTTCATCTTGCACGTGGTAGATCTGTAATGGTAAATTTGGTGCCATCAGGGGTTGTGGAGCTTTTGGAGGATGAGATTACCCAGGCAGAGAGTTTTCAGGAAATGTTTCTTAAGGTCATTTTGGACAGATCAGAATTTGTTCAGGAGTTTATTCCTTTGAGGAATAATGTCTCTAAATCTATTTCATCAACATCTTACTTGTTGCTTCCTCTGATTTTTCATGATAACGAGAGATCAGTTTCTATAGATTGGAAAGTTATTAGAAGATGTTTATTCTCCCCAATTTTCCAGATTCCTGGCTCTATTGTCAAGGAAACTGCTTGCTCGCATGCCCACTTGACTCTTTATGATGGCCGTAGAAGGACAAGTGATATCAAGAATAGTTTAGTGTATGTCCCGTACAAGCGAGAATTCTTTTTTGTTACTGACATTGTCAAAGGGAAGAATGGATACAGTCGAAGTCACTATAAGAACTCAAGCTCTTTAAGTCATTTTGAGCATTTAATTGAAAGGTTTGGCATTCATCTTAAATATCCTGAGCAGCCTCTTCTTTCTGCAAAACCCCTATTCCATTTGCACAACTTGCTACATGATAGACGGCAGGGCAATTCTGAAGCATGTTATCTGCAAGAGTATTTCATTGATTTGCCTCCCGAGCTTTGTCAGTTGAAGATAATTGGCTTTTCAAAAGATATCGGGAGCTCCATATCTTTGTTGCCATCAATTATGCATCGTCTAGAAAACTTGCTTGTTGCTATTGAATTGAAGTGTATGTTAGCAGCTGCTTTTCCAGATGGAGCCGAGGTTACTGCCAATAGAATTCTTGAAGCACTCACCACTGAGAAGTGCCAGGAACGCATTTCCCTGGAAAGGCTTGAAATACTTGGTGACGGTTTCCTTAAATTTGCAGTAGCAAGATACCTTTTTCTTACACACGACAAGTCTGATGAAGGAGAACTTACTAGAAGGCGTTCTTATTTAGTGAAAAATATCAATTTGTTCAAGCTAGCAACTAGAAAGAACTTACAGGTATACATACGTGACCAGCCATTTGAGCCTAGTCAATTTTATACACTTGGTCGTCCTTGCCCAAGAATATGTAATGAGGAAACTAGAGAAGATATTCATTCGCACTGTAATGCAACAAAAAATGCAAAAGCCAGTGAGACCAAGTGCAGTAGAGGCCATCATTGGTTACATAAGAAAACAATCAGCGACGTGGTTGAAGCTCTGGTTGGGGCGTTCCTAGTTGACAGTGGCTTCAAAGCTGCAATTTCATTTCTAAAGTGGATCGGCATACAAGTAGAATTTGAAGCTTCACTAGTTACCAATGCTCTCATGTCAAGCAATGCTTACATCATACTTGCAGATAGAATAAACATTTCTGTACTTCAAAACTCATTAGGCCATCAATTTCTCCATAAAGGCCTGCTGCTGCAGGCACTCGTACATCCTTCTTACCACAAGCATGGAGGAGGTTGTTACCAGAGATTAGAGTTTCTTGGAGATGCTGTCCTGGATTACTTGATCACGTCATATCTATATTCAGCATATCCTAAGCTGAAGCCGGGTCTATTGACCGATTTGAGGTCAGTGTTTGTGAGGAATGAGGCCTTTGCCAATGTTGCAGTAGATAGATCTTTCTACAAGTATCTGATTTGTGATTCTACTAGCCTTCAAAGTGATATAAAAAGTTACGTAAACTTCATCAAAGCACCTTCTTCTGGAAGTGGTTCACTTGGGCTTCCGAGTTGTCCAAAGGCTTTAGGAGATTTGGTTGAATCTTGTGTTGGGGCCGTTCTTGTTGATACAGGGTTCGACATGAACTATGTTTGGAAGATAATGCTATCTTTTCTGGATCCTATTATAAGCTTTTCAGGCTTCCAGCTTAGTCCTATTCGAGACATAATAGAATTTTGTCAAGATTGTGGCTCGAACCTTCAGTTTAATGAATCAAAAACAGGAAAATATTATTCTGTTGAAGCAGAGGTAAAAGGAGATAACTTTCATGCCACTGCTTCTGCAGCCCATCGGCGAAAGAGAGATGCCAAGAAAATTGCTGCCAACCTCATACTCACCAAGTTAAAGGCCCAGGGACTCATACCAGAGGTCAATTCATTGGAGGAAATTTTAAAGTCAAGCAGGAAGATGGAACCTAAATTGATTGGATATGATGAAACACCCTCTGTCACGATCGATCAAGCTGATAATGGTTTTGGAACAATAAAGGGGTTGGAATCTTCCGGTGCAGACTTAGATCCTTTTGTGCACAGTGAAGTTGGCAATGCTCATCCAGCACGCATTACACGGATAAGCAAAATGCTCGTATCTTCATCTGGAGCTGCACAAGAACAACTGGATCCTAGTCTGGCCTTTGATGCTGTTTCTTTAGGTCGGTTGTGCAAAGCAACAGCCAGATCACGTCTGTATGAAATCTGTGCTGCAAACCATTGGAGTCCCCCTTCATTTGATTGCATGAATGAGGAAGGACCAAGCCATTTGAAGATGTTCACTTACAAGGTTGTTTTAGAGATAGAAGAAGCCCCCAATACGATTTTCAAATTCTTCGGAGCACCTCATTTGAGGAAGAAGGCTGCGGCTGAGCATGCAGCCGAAGCAGCTATTTGGTACTTGAAAAAGGCTGGGTACTTGCCTGAGACAGAT

Coding sequence (CDS)

ATGCCTGATGGGGATACTAATTCTATGGCTGCCGATAGCGACAATTTTCAACACACTAGTTTGGGGCCTGTTGAGAGTTTTGTGGGTCTGTCGTTGGTGGATGATCAAGAACCGGGTCCAAGCACAAGAGCATCAGAGAAAGATCCCAGGAGAAAAGCAAGGAAATATCAATTGGAGCTGTGCAAGAAAGCTTTGGAAGAGAATATCATTCTGTATCTTGGGACAGGATGTGGGAAGACACATATCGCTGTACTTCTGATGTACGAGATGAGGCACTTAATCAGGAGGTCCCAGAAGGGTATATGTGTTTTTCTGGCACCTACAGTTGCTTTAGTCCAGCAGCAAGCAAAGGTTATAGAAGACTCTACAGATTTCAAGGTCAGGGTCTACTGTGGCGGTTCCAAGTGCTTGAATAGCCATTATGATTGGGAAAGAGAGATGGAAGAACATGAGGTTTTTGTCATGACCCCTGAAATATTACTGCGCAATTTACACCACTGCTATATCAAGATGGAGTTTGTTGAACTTCTAATATTTGATGAATGCCATCATGCTCAAGTCAAAAGTGACCATTCTTATGCAGAAATCATGAGAGTGTTCTATAAAACAAATGTGGCTAAACGACCCCGGATATTTGGCATGACTGCATCTCCAGTTGTAGGAAAAGATCATTGTTCTACTTCTACTAATTCAGGTGCTTGCAATCAGCAAAATTTATCTAAAAGCATCAATAGTCTTGAGAAGTTACTTGATGCAAAGGTTTATTCAGTTGAAGATAGGGAAGAACTGGATGCATTTGTGTCATCGCCACTTGTTAAAATATATTATTATGGTCCTGTTGCAAATGGGTTATCTGGCTCATTTATGACTTACTGTAGCCTACTCGAGGACATGAAACGCCAGTGCATAGTGGTTCTTGGTCAGATGAAAGGAGAGCATGAAGATCTGCGGGCCACCAAGAAATTATTATGCCGAATGCATGATAATATCATATTTTGTTTGGAAAACCTTGGTGTTTGGGGGGCATTGCAGGCTAGTAAAATTCTTCTAAGTGGAGATAACTCTGAGCGAAGTGAATTGATAGAGGCAGCAGAGGGAAACCCAAATAATCACTCCTTAAGTGATAGATACCTTAATCAAGCTGCTGAAATTCTTGCCAAAGGTTGCAAAATAGGTGGTGGAATGTCAGATACTTTAAATGCTGATATTTTGGAGTATCCATTTTTCTCAAAGAAGCTTTTACAACTTATTGAAATTCTTTCTTCCTTCAGGCATCGACTCAACATGAAATGTATTATTTTTGTAAATAGAATAGTTATAGCACGGTCCTTGTCATACATATTGCAGAACCTCGAGTTTCTGGCATTTTGGAAGTGTGATTATCTTGTAGGAGTCCACTCTAAATTGAGGAGCATGTCAAGGAAGACCATGAACCATATTCTTATGAAGTTCCGGTCAGGGGAGCTAAACCTCTTGGTTGCGACAAAGGTGGGTGAAGAAGGGCTTGATATTCAGACTTGCTGCCTCGTGATACGATTTGATCTTCCCGAAACAGTATCTAGCTTTATACAATCAAGAGGTCGAGCACGAATGCCACAGTCTGAATATGCATTCCTTGTTGATAGTGGAAATGAAAAGGAGCTAGGATTGATAGACGAATTTCAGAAAGATGAGAATCGGATGGACAGGGAAATTTCTTCTCGCTCATCCAGTGAGATATTTAATAGTCATGAAGAAAGCATCTATAGAGTTGCTTCAACTGGTGCATCTATCACTTCTGGACGTAGTATATCGTTACTGCATGAATTTTGCTCAAAACTTCCACATGATGAGTACTTTGATCCCAAGCCAAAGTTTTTCTATTATGATGATCTAGCAGGAACTGTATGTCATGCTATTCTGCCCTCCAATGCTCCAATACACCAAATTATCAGCAGAACACAACCTTCTAGGGAAGGTGCTAAAAAGGATGCTTGTATAAAAGCTATTGAGGAATTGCATAAACTTGGTGCTCTCAATGACTTTCTTTTGCTAATGAGAGACGACGCCAATGAAGAAGAATTGGAACTTGACTCCTCTGATTCTGACAGATCTGAAGATGAGACTTCACGAGGAGAACTTCATGAGATGTTAGTTCCTGCTGCTCTAAAAGATTCTTGGACTGGCTCGGGATATTTTATTCTCAACTGTTACCAAATTAAATTCAGTCCACATCCTAGAGATAGGATCTATAAAGAATTTGGTTTATTTGTCAAGGCACCACTTCCCCAGGAGGCTGAGAGAATGGACATTGAGCTTCATCTTGCACGTGGTAGATCTGTAATGGTAAATTTGGTGCCATCAGGGGTTGTGGAGCTTTTGGAGGATGAGATTACCCAGGCAGAGAGTTTTCAGGAAATGTTTCTTAAGGTCATTTTGGACAGATCAGAATTTGTTCAGGAGTTTATTCCTTTGAGGAATAATGTCTCTAAATCTATTTCATCAACATCTTACTTGTTGCTTCCTCTGATTTTTCATGATAACGAGAGATCAGTTTCTATAGATTGGAAAGTTATTAGAAGATGTTTATTCTCCCCAATTTTCCAGATTCCTGGCTCTATTGTCAAGGAAACTGCTTGCTCGCATGCCCACTTGACTCTTTATGATGGCCGTAGAAGGACAAGTGATATCAAGAATAGTTTAGTGTATGTCCCGTACAAGCGAGAATTCTTTTTTGTTACTGACATTGTCAAAGGGAAGAATGGATACAGTCGAAGTCACTATAAGAACTCAAGCTCTTTAAGTCATTTTGAGCATTTAATTGAAAGGTTTGGCATTCATCTTAAATATCCTGAGCAGCCTCTTCTTTCTGCAAAACCCCTATTCCATTTGCACAACTTGCTACATGATAGACGGCAGGGCAATTCTGAAGCATGTTATCTGCAAGAGTATTTCATTGATTTGCCTCCCGAGCTTTGTCAGTTGAAGATAATTGGCTTTTCAAAAGATATCGGGAGCTCCATATCTTTGTTGCCATCAATTATGCATCGTCTAGAAAACTTGCTTGTTGCTATTGAATTGAAGTGTATGTTAGCAGCTGCTTTTCCAGATGGAGCCGAGGTTACTGCCAATAGAATTCTTGAAGCACTCACCACTGAGAAGTGCCAGGAACGCATTTCCCTGGAAAGGCTTGAAATACTTGGTGACGGTTTCCTTAAATTTGCAGTAGCAAGATACCTTTTTCTTACACACGACAAGTCTGATGAAGGAGAACTTACTAGAAGGCGTTCTTATTTAGTGAAAAATATCAATTTGTTCAAGCTAGCAACTAGAAAGAACTTACAGGTATACATACGTGACCAGCCATTTGAGCCTAGTCAATTTTATACACTTGGTCGTCCTTGCCCAAGAATATGTAATGAGGAAACTAGAGAAGATATTCATTCGCACTGTAATGCAACAAAAAATGCAAAAGCCAGTGAGACCAAGTGCAGTAGAGGCCATCATTGGTTACATAAGAAAACAATCAGCGACGTGGTTGAAGCTCTGGTTGGGGCGTTCCTAGTTGACAGTGGCTTCAAAGCTGCAATTTCATTTCTAAAGTGGATCGGCATACAAGTAGAATTTGAAGCTTCACTAGTTACCAATGCTCTCATGTCAAGCAATGCTTACATCATACTTGCAGATAGAATAAACATTTCTGTACTTCAAAACTCATTAGGCCATCAATTTCTCCATAAAGGCCTGCTGCTGCAGGCACTCGTACATCCTTCTTACCACAAGCATGGAGGAGGTTGTTACCAGAGATTAGAGTTTCTTGGAGATGCTGTCCTGGATTACTTGATCACGTCATATCTATATTCAGCATATCCTAAGCTGAAGCCGGGTCTATTGACCGATTTGAGGTCAGTGTTTGTGAGGAATGAGGCCTTTGCCAATGTTGCAGTAGATAGATCTTTCTACAAGTATCTGATTTGTGATTCTACTAGCCTTCAAAGTGATATAAAAAGTTACGTAAACTTCATCAAAGCACCTTCTTCTGGAAGTGGTTCACTTGGGCTTCCGAGTTGTCCAAAGGCTTTAGGAGATTTGGTTGAATCTTGTGTTGGGGCCGTTCTTGTTGATACAGGGTTCGACATGAACTATGTTTGGAAGATAATGCTATCTTTTCTGGATCCTATTATAAGCTTTTCAGGCTTCCAGCTTAGTCCTATTCGAGACATAATAGAATTTTGTCAAGATTGTGGCTCGAACCTTCAGTTTAATGAATCAAAAACAGGAAAATATTATTCTGTTGAAGCAGAGGTAAAAGGAGATAACTTTCATGCCACTGCTTCTGCAGCCCATCGGCGAAAGAGAGATGCCAAGAAAATTGCTGCCAACCTCATACTCACCAAGTTAAAGGCCCAGGGACTCATACCAGAGGTCAATTCATTGGAGGAAATTTTAAAGTCAAGCAGGAAGATGGAACCTAAATTGATTGGATATGATGAAACACCCTCTGTCACGATCGATCAAGCTGATAATGGTTTTGGAACAATAAAGGGGTTGGAATCTTCCGGTGCAGACTTAGATCCTTTTGTGCACAGTGAAGTTGGCAATGCTCATCCAGCACGCATTACACGGATAAGCAAAATGCTCGTATCTTCATCTGGAGCTGCACAAGAACAACTGGATCCTAGTCTGGCCTTTGATGCTGTTTCTTTAGGTCGGTTGTGCAAAGCAACAGCCAGATCACGTCTGTATGAAATCTGTGCTGCAAACCATTGGAGTCCCCCTTCATTTGATTGCATGAATGAGGAAGGACCAAGCCATTTGAAGATGTTCACTTACAAGGTTGTTTTAGAGATAGAAGAAGCCCCCAATACGATTTTCAAATTCTTCGGAGCACCTCATTTGAGGAAGAAGGCTGCGGCTGAGCATGCAGCCGAAGCAGCTATTTGGTACTTGAAAAAGGCTGGGTACTTGCCTGAGACAGAT

Protein sequence

MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPSTRASEKDPRRKARKYQLELCKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIEDSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFDECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLSKSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKRQCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSWTGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHDNERSVSIDWKVIRRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDIHSHCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCYQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHATASAAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTIDQADNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFDAVSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEAPNTIFKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYLPETD
Homology
BLAST of MS002366 vs. NCBI nr
Match: XP_022134939.1 (dicer-like protein 4 isoform X1 [Momordica charantia])

HSP 1 Score: 3233.0 bits (8381), Expect = 0.0e+00
Identity = 1631/1661 (98.19%), Postives = 1636/1661 (98.49%), Query Frame = 0

Query: 1    MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPSTRASEKDPRRKARKYQLEL 60
            MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPS+RASEKDPRRKARKYQLEL
Sbjct: 2    MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPSSRASEKDPRRKARKYQLEL 61

Query: 61   CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE 120
            CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE
Sbjct: 62   CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE 121

Query: 121  DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFD 180
            DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLR LHHCYIKMEFVELLIFD
Sbjct: 122  DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRTLHHCYIKMEFVELLIFD 181

Query: 181  ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLS 240
            ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGK         GACNQQNLS
Sbjct: 182  ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGK---------GACNQQNLS 241

Query: 241  KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKR 300
            KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKR
Sbjct: 242  KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKR 301

Query: 301  QCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSEL 360
            QCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSEL
Sbjct: 302  QCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSEL 361

Query: 361  IEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEIL 420
            IEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEIL
Sbjct: 362  IEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEIL 421

Query: 421  SSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHI 480
            SSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHI
Sbjct: 422  SSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHI 481

Query: 481  LMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540
            LMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV
Sbjct: 482  LMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 541

Query: 541  DSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLL 600
            DSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLL
Sbjct: 542  DSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLL 601

Query: 601  HEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACI 660
            HEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACI
Sbjct: 602  HEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACI 661

Query: 661  KAIEELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSW 720
            KAIEELHKLGALNDFLL MRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSW
Sbjct: 662  KAIEELHKLGALNDFLLPMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSW 721

Query: 721  TGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVP 780
            TGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKA LPQEAERMDIELHLARGRSVMVNLVP
Sbjct: 722  TGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAALPQEAERMDIELHLARGRSVMVNLVP 781

Query: 781  SGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHD 840
            SGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHD
Sbjct: 782  SGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHD 841

Query: 841  NERSVSIDWKVIRRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYK 900
            NERSVSIDWKVIRRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYK
Sbjct: 842  NERSVSIDWKVIRRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYK 901

Query: 901  REFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNL 960
            REFFFVTDIVKGKNGYSRSHYKNSSS SHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNL
Sbjct: 902  REFFFVTDIVKGKNGYSRSHYKNSSSSSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNL 961

Query: 961  LHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIEL 1020
            LHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIEL
Sbjct: 962  LHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIEL 1021

Query: 1021 KCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKS 1080
            KCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKS
Sbjct: 1022 KCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKS 1081

Query: 1081 DEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDI 1140
            DEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDI
Sbjct: 1082 DEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDI 1141

Query: 1141 HSHCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQV 1200
            HSHCNATKNA+ASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQV
Sbjct: 1142 HSHCNATKNAQASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQV 1201

Query: 1201 EFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCY 1260
            EFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCY
Sbjct: 1202 EFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCY 1261

Query: 1261 QRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICD 1320
            QRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICD
Sbjct: 1262 QRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICD 1321

Query: 1321 STSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIM 1380
            STSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIM
Sbjct: 1322 STSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIM 1381

Query: 1381 LSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHATASA 1440
            LSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEA+VKGDNFHATASA
Sbjct: 1382 LSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEADVKGDNFHATASA 1441

Query: 1441 AHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTIDQA 1500
            AHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVT+DQA
Sbjct: 1442 AHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTMDQA 1501

Query: 1501 DNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFDA- 1560
            DNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFD  
Sbjct: 1502 DNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFDGH 1561

Query: 1561 -------VSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEE 1620
                    S GRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEE
Sbjct: 1562 DSPTDVQSSSGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEE 1621

Query: 1621 APNTIFKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYLPETD 1654
            AP+TIFKFFGAPHLRKKAAAEHAAEAAIWYLKK GYLPETD
Sbjct: 1622 APDTIFKFFGAPHLRKKAAAEHAAEAAIWYLKKEGYLPETD 1653

BLAST of MS002366 vs. NCBI nr
Match: XP_022134940.1 (dicer-like protein 4 isoform X2 [Momordica charantia])

HSP 1 Score: 2755.3 bits (7141), Expect = 0.0e+00
Identity = 1389/1409 (98.58%), Postives = 1394/1409 (98.94%), Query Frame = 0

Query: 253  KVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKRQCIVVLGQMKGE 312
            +VYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKRQCIVVLGQMKGE
Sbjct: 10   EVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKRQCIVVLGQMKGE 69

Query: 313  HEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNHS 372
            HEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNHS
Sbjct: 70   HEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNHS 129

Query: 373  LSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCI 432
            LSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCI
Sbjct: 130  LSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCI 189

Query: 433  IFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLL 492
            IFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLL
Sbjct: 190  IFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLL 249

Query: 493  VATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLID 552
            VATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLID
Sbjct: 250  VATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLID 309

Query: 553  EFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEY 612
            EFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEY
Sbjct: 310  EFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEY 369

Query: 613  FDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGAL 672
            FDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGAL
Sbjct: 370  FDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGAL 429

Query: 673  NDFLLLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSWTGSGYFILNCYQ 732
            NDFLL MRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSWTGSGYFILNCYQ
Sbjct: 430  NDFLLPMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSWTGSGYFILNCYQ 489

Query: 733  IKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEIT 792
            IKFSPHPRDRIYKEFGLFVKA LPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEIT
Sbjct: 490  IKFSPHPRDRIYKEFGLFVKAALPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEIT 549

Query: 793  QAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHDNERSVSIDWKVI 852
            QAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHDNERSVSIDWKVI
Sbjct: 550  QAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHDNERSVSIDWKVI 609

Query: 853  RRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKG 912
            RRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKG
Sbjct: 610  RRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKG 669

Query: 913  KNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEAC 972
            KNGYSRSHYKNSSS SHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEAC
Sbjct: 670  KNGYSRSHYKNSSSSSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEAC 729

Query: 973  YLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGA 1032
            YLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGA
Sbjct: 730  YLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGA 789

Query: 1033 EVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYL 1092
            EVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYL
Sbjct: 790  EVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYL 849

Query: 1093 VKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDIHSHCNATKNAKA 1152
            VKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDIHSHCNATKNA+A
Sbjct: 850  VKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDIHSHCNATKNAQA 909

Query: 1153 SETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALM 1212
            SETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALM
Sbjct: 910  SETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALM 969

Query: 1213 SSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCYQRLEFLGDAVLD 1272
            SSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCYQRLEFLGDAVLD
Sbjct: 970  SSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCYQRLEFLGDAVLD 1029

Query: 1273 YLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYV 1332
            YLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYV
Sbjct: 1030 YLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYV 1089

Query: 1333 NFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSG 1392
            NFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSG
Sbjct: 1090 NFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSG 1149

Query: 1393 FQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHATASAAHRRKRDAKKIA 1452
            FQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEA+VKGDNFHATASAAHRRKRDAKKIA
Sbjct: 1150 FQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEADVKGDNFHATASAAHRRKRDAKKIA 1209

Query: 1453 ANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTIDQADNGFGTIKGLES 1512
            ANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVT+DQADNGFGTIKGLES
Sbjct: 1210 ANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTMDQADNGFGTIKGLES 1269

Query: 1513 SGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFDA--------VSLGR 1572
            SGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFD          S GR
Sbjct: 1270 SGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFDGHDSPTDVQSSSGR 1329

Query: 1573 LCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEAPNTIFKFFGAP 1632
            LCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEAP+TIFKFFGAP
Sbjct: 1330 LCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEAPDTIFKFFGAP 1389

Query: 1633 HLRKKAAAEHAAEAAIWYLKKAGYLPETD 1654
            HLRKKAAAEHAAEAAIWYLKK GYLPETD
Sbjct: 1390 HLRKKAAAEHAAEAAIWYLKKEGYLPETD 1418

BLAST of MS002366 vs. NCBI nr
Match: XP_038879795.1 (dicer-like protein 4 isoform X2 [Benincasa hispida])

HSP 1 Score: 2713.7 bits (7033), Expect = 0.0e+00
Identity = 1385/1662 (83.33%), Postives = 1484/1662 (89.29%), Query Frame = 0

Query: 1    MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPSTRASEKDPRRKARKYQLEL 60
            MPDG+TNS   DS NFQ T   PVE F+GLS  DDQ  G STR SEKDPRR ARKYQLEL
Sbjct: 1    MPDGETNSTTDDSVNFQPTGFVPVERFMGLSQNDDQAAGSSTRTSEKDPRRIARKYQLEL 60

Query: 61   CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE 120
            CKKALEENII+YLGTGCGKTHIA+LL+YEM HLIRRSQKGICVFLAPTVALVQQQAKVIE
Sbjct: 61   CKKALEENIIVYLGTGCGKTHIAILLIYEMSHLIRRSQKGICVFLAPTVALVQQQAKVIE 120

Query: 121  DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFD 180
            DSTDFKVRVYCGGSKCLN+HYDWE EMEE+EVFVMTPEILLRNLHHCYIKM+FVELLIFD
Sbjct: 121  DSTDFKVRVYCGGSKCLNTHYDWETEMEEYEVFVMTPEILLRNLHHCYIKMDFVELLIFD 180

Query: 181  ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLS 240
            ECHHAQVKSDHSYAEIMRVFYK N  KRPRIFGMTASPVVGK         GACNQQNLS
Sbjct: 181  ECHHAQVKSDHSYAEIMRVFYKANEPKRPRIFGMTASPVVGK---------GACNQQNLS 240

Query: 241  KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKR 300
            +SINSLEKLLDAKVYSVE REELDAFV SPLVKIYYYGPVANG S SFMTYC LLED+K 
Sbjct: 241  RSINSLEKLLDAKVYSVEAREELDAFVLSPLVKIYYYGPVANGSSSSFMTYCCLLEDIKH 300

Query: 301  QCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSEL 360
            +CI+ LGQMK EHE L ATK+LLCRMH NIIFCLE+LGVWGALQASKILLSGDNSE+SEL
Sbjct: 301  KCILALGQMKCEHEVLLATKRLLCRMHKNIIFCLESLGVWGALQASKILLSGDNSEQSEL 360

Query: 361  IEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEIL 420
            IEAAEGN  N SLSD+YLNQAAE+ A GCKI GGMSD LN DILE PFFSKKLL+LI IL
Sbjct: 361  IEAAEGNLKNDSLSDKYLNQAAEVFASGCKIDGGMSDMLNVDILEDPFFSKKLLRLIGIL 420

Query: 421  SSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHI 480
            SSFRHRLNMKCIIFVNRIVIARSLSYILQNL FL++WKCD+LVGVHSKLRSMSRKTM+HI
Sbjct: 421  SSFRHRLNMKCIIFVNRIVIARSLSYILQNLNFLSYWKCDFLVGVHSKLRSMSRKTMSHI 480

Query: 481  LMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540
            L KF+SGELNLL+ATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV
Sbjct: 481  LTKFQSGELNLLIATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540

Query: 541  DSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLL 600
            DSGNEKELGLIDEFQKDENRM+REISSRSS+E F SHEESIYRV STGASITSGRSISLL
Sbjct: 541  DSGNEKELGLIDEFQKDENRMNREISSRSSNETFCSHEESIYRVVSTGASITSGRSISLL 600

Query: 601  HEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACI 660
            HE+CSKLPHDEYFDPKPKFFYYDDL GTVCHAILPSNAPI QI+S  Q SR+ AKK AC+
Sbjct: 601  HEYCSKLPHDEYFDPKPKFFYYDDLEGTVCHAILPSNAPITQIVSGAQSSRDAAKKVACL 660

Query: 661  KAIEELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSW 720
            KA+EELHKLGALND+LL MRD+A+E+ELELDSSDSD S+DE SR ELHEM+ PAALK+SW
Sbjct: 661  KAVEELHKLGALNDYLLPMRDNADEKELELDSSDSDSSKDENSRRELHEMIFPAALKESW 720

Query: 721  TGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVP 780
            TGS Y +L CY IKF+PHPRDRIYKEFGLFVKAPLP+EAERMD+ELHLAR RSV VNL+P
Sbjct: 721  TGSEYLVLYCYHIKFTPHPRDRIYKEFGLFVKAPLPEEAERMDLELHLARRRSVRVNLMP 780

Query: 781  SGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHD 840
            SGVVELLEDEI QAESFQEMFLKVILDRSEFVQE+IPLRNN S+SIS TSYLLLP+ FHD
Sbjct: 781  SGVVELLEDEIIQAESFQEMFLKVILDRSEFVQEYIPLRNNDSRSISPTSYLLLPVTFHD 840

Query: 841  NERSVSIDWKVIRRCLFSPIFQIPG-SIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPY 900
            NE SV IDW+VI+RCL S IFQ    SIVKE A S+ HLTLYDGRRR++DI+NSLVYVPY
Sbjct: 841  NEESVIIDWRVIKRCLSSQIFQNHACSIVKEAASSYTHLTLYDGRRRSNDIENSLVYVPY 900

Query: 901  KREFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHN 960
            K EFFFVT+IV GKNGYS+  YKNS S SHFEHL  +FGIHLK PEQPLL AKPLF LHN
Sbjct: 901  KGEFFFVTNIVWGKNGYSQ--YKNSGSSSHFEHLRSKFGIHLKSPEQPLLCAKPLFFLHN 960

Query: 961  LLHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIE 1020
            LLH+R+Q +S+A YL+EYFIDLPPELCQLKI GFSKDIGSSISLLPSIMHRLENLLVAIE
Sbjct: 961  LLHNRKQEDSDAHYLEEYFIDLPPELCQLKICGFSKDIGSSISLLPSIMHRLENLLVAIE 1020

Query: 1021 LKCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDK 1080
            LKC LAAAFP GAEVTANRILEALTTEKCQERISLERLEILGD FLKFAVARYLFLTHDK
Sbjct: 1021 LKCRLAAAFPAGAEVTANRILEALTTEKCQERISLERLEILGDSFLKFAVARYLFLTHDK 1080

Query: 1081 SDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETRED 1140
             DEGELTRRRSYLVKN NL KLATRKNLQVYIRDQPFEPSQFY LGRPCPRICNEETR+D
Sbjct: 1081 FDEGELTRRRSYLVKNFNLLKLATRKNLQVYIRDQPFEPSQFYLLGRPCPRICNEETRKD 1140

Query: 1141 IHSHCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQ 1200
            IHSH +AT N +ASETKCS+GHHWL KKTISDVVEALVGAFLVDSGFKAAI+FLKWIGIQ
Sbjct: 1141 IHSHDDAT-NTRASETKCSKGHHWLQKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIQ 1200

Query: 1201 VEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGC 1260
            VEFE+SLVT+ALM+SN Y++LADR +IS  Q+SLGHQFLHKGLLLQALVHPSYHKHGGGC
Sbjct: 1201 VEFESSLVTDALMASNNYVLLADRTDISAFQDSLGHQFLHKGLLLQALVHPSYHKHGGGC 1260

Query: 1261 YQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLIC 1320
            YQRLEFLGDAVLDYLITSYLYSAYPKLKPG LTDLRSVFVRNEAFANV VDR FYK+L+C
Sbjct: 1261 YQRLEFLGDAVLDYLITSYLYSAYPKLKPGQLTDLRSVFVRNEAFANVTVDRFFYKFLLC 1320

Query: 1321 DSTSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKI 1380
            DSTSL SDIKSYV+FIK+PS    SL  P CPKALGDLVES VGAVLVDTGFDMNYVWKI
Sbjct: 1321 DSTSLLSDIKSYVDFIKSPSE-RDSLEQPRCPKALGDLVESSVGAVLVDTGFDMNYVWKI 1380

Query: 1381 MLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHATAS 1440
            MLSF+DPI+SFSGFQLSPIRD+IEFCQ+CG  L+FN SK   YYSV+AEVKG NFH TAS
Sbjct: 1381 MLSFVDPIMSFSGFQLSPIRDLIEFCQNCGWKLEFNSSKMEGYYSVKAEVKGGNFHDTAS 1440

Query: 1441 AAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTIDQ 1500
            AA+RRK+DA+KIAANLILTKLKA+G +PEVNSLEEILKSS +MEPKLIGYDETP   +DQ
Sbjct: 1441 AANRRKKDAEKIAANLILTKLKAKGFLPEVNSLEEILKSSSRMEPKLIGYDETPFGMVDQ 1500

Query: 1501 ADNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFDA 1560
             DNG  TI G E S ADL+P VH EV N+HP  ITRIS+  VSSS AA EQL PS+A + 
Sbjct: 1501 VDNGLETINGPEFSNADLNPRVHREVDNSHPVHITRISRTPVSSSVAAGEQLKPSMALEG 1560

Query: 1561 --------VSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIE 1620
                     S G   K TARSRLYEICAANHW+ PSFDCMNEEGPSHLKMFTYKVVLE+E
Sbjct: 1561 HDSPADLQSSSGWSGKTTARSRLYEICAANHWNRPSFDCMNEEGPSHLKMFTYKVVLEME 1620

Query: 1621 EAPNTIFKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYLPETD 1654
            EAP+TIF+F GAPHLRKKAAAEHAAEAA+WYLKKAGYLPETD
Sbjct: 1621 EAPDTIFEFVGAPHLRKKAAAEHAAEAALWYLKKAGYLPETD 1649

BLAST of MS002366 vs. NCBI nr
Match: XP_038879794.1 (dicer-like protein 4 isoform X1 [Benincasa hispida])

HSP 1 Score: 2711.4 bits (7027), Expect = 0.0e+00
Identity = 1386/1663 (83.34%), Postives = 1485/1663 (89.30%), Query Frame = 0

Query: 1    MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPSTRASEKDPRRKARKYQLEL 60
            MPDG+TNS   DS NFQ T   PVE F+GLS  DDQ  G STR SEKDPRR ARKYQLEL
Sbjct: 1    MPDGETNSTTDDSVNFQPTGFVPVERFMGLSQNDDQAAGSSTRTSEKDPRRIARKYQLEL 60

Query: 61   CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE 120
            CKKALEENII+YLGTGCGKTHIA+LL+YEM HLIRRSQKGICVFLAPTVALVQQQAKVIE
Sbjct: 61   CKKALEENIIVYLGTGCGKTHIAILLIYEMSHLIRRSQKGICVFLAPTVALVQQQAKVIE 120

Query: 121  DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFD 180
            DSTDFKVRVYCGGSKCLN+HYDWE EMEE+EVFVMTPEILLRNLHHCYIKM+FVELLIFD
Sbjct: 121  DSTDFKVRVYCGGSKCLNTHYDWETEMEEYEVFVMTPEILLRNLHHCYIKMDFVELLIFD 180

Query: 181  ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLS 240
            ECHHAQVKSDHSYAEIMRVFYK N  KRPRIFGMTASPVVGK         GACNQQNLS
Sbjct: 181  ECHHAQVKSDHSYAEIMRVFYKANEPKRPRIFGMTASPVVGK---------GACNQQNLS 240

Query: 241  KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMK- 300
            +SINSLEKLLDAKVYSVE REELDAFV SPLVKIYYYGPVANG S SFMTYC LLED+K 
Sbjct: 241  RSINSLEKLLDAKVYSVEAREELDAFVLSPLVKIYYYGPVANGSSSSFMTYCCLLEDIKH 300

Query: 301  RQCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSE 360
            +QCI+ LGQMK EHE L ATK+LLCRMH NIIFCLE+LGVWGALQASKILLSGDNSE+SE
Sbjct: 301  KQCILALGQMKCEHEVLLATKRLLCRMHKNIIFCLESLGVWGALQASKILLSGDNSEQSE 360

Query: 361  LIEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEI 420
            LIEAAEGN  N SLSD+YLNQAAE+ A GCKI GGMSD LN DILE PFFSKKLL+LI I
Sbjct: 361  LIEAAEGNLKNDSLSDKYLNQAAEVFASGCKIDGGMSDMLNVDILEDPFFSKKLLRLIGI 420

Query: 421  LSSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNH 480
            LSSFRHRLNMKCIIFVNRIVIARSLSYILQNL FL++WKCD+LVGVHSKLRSMSRKTM+H
Sbjct: 421  LSSFRHRLNMKCIIFVNRIVIARSLSYILQNLNFLSYWKCDFLVGVHSKLRSMSRKTMSH 480

Query: 481  ILMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFL 540
            IL KF+SGELNLL+ATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFL
Sbjct: 481  ILTKFQSGELNLLIATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFL 540

Query: 541  VDSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISL 600
            VDSGNEKELGLIDEFQKDENRM+REISSRSS+E F SHEESIYRV STGASITSGRSISL
Sbjct: 541  VDSGNEKELGLIDEFQKDENRMNREISSRSSNETFCSHEESIYRVVSTGASITSGRSISL 600

Query: 601  LHEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDAC 660
            LHE+CSKLPHDEYFDPKPKFFYYDDL GTVCHAILPSNAPI QI+S  Q SR+ AKK AC
Sbjct: 601  LHEYCSKLPHDEYFDPKPKFFYYDDLEGTVCHAILPSNAPITQIVSGAQSSRDAAKKVAC 660

Query: 661  IKAIEELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDS 720
            +KA+EELHKLGALND+LL MRD+A+E+ELELDSSDSD S+DE SR ELHEM+ PAALK+S
Sbjct: 661  LKAVEELHKLGALNDYLLPMRDNADEKELELDSSDSDSSKDENSRRELHEMIFPAALKES 720

Query: 721  WTGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLV 780
            WTGS Y +L CY IKF+PHPRDRIYKEFGLFVKAPLP+EAERMD+ELHLAR RSV VNL+
Sbjct: 721  WTGSEYLVLYCYHIKFTPHPRDRIYKEFGLFVKAPLPEEAERMDLELHLARRRSVRVNLM 780

Query: 781  PSGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFH 840
            PSGVVELLEDEI QAESFQEMFLKVILDRSEFVQE+IPLRNN S+SIS TSYLLLP+ FH
Sbjct: 781  PSGVVELLEDEIIQAESFQEMFLKVILDRSEFVQEYIPLRNNDSRSISPTSYLLLPVTFH 840

Query: 841  DNERSVSIDWKVIRRCLFSPIFQIPG-SIVKETACSHAHLTLYDGRRRTSDIKNSLVYVP 900
            DNE SV IDW+VI+RCL S IFQ    SIVKE A S+ HLTLYDGRRR++DI+NSLVYVP
Sbjct: 841  DNEESVIIDWRVIKRCLSSQIFQNHACSIVKEAASSYTHLTLYDGRRRSNDIENSLVYVP 900

Query: 901  YKREFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLH 960
            YK EFFFVT+IV GKNGYS+  YKNS S SHFEHL  +FGIHLK PEQPLL AKPLF LH
Sbjct: 901  YKGEFFFVTNIVWGKNGYSQ--YKNSGSSSHFEHLRSKFGIHLKSPEQPLLCAKPLFFLH 960

Query: 961  NLLHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAI 1020
            NLLH+R+Q +S+A YL+EYFIDLPPELCQLKI GFSKDIGSSISLLPSIMHRLENLLVAI
Sbjct: 961  NLLHNRKQEDSDAHYLEEYFIDLPPELCQLKICGFSKDIGSSISLLPSIMHRLENLLVAI 1020

Query: 1021 ELKCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHD 1080
            ELKC LAAAFP GAEVTANRILEALTTEKCQERISLERLEILGD FLKFAVARYLFLTHD
Sbjct: 1021 ELKCRLAAAFPAGAEVTANRILEALTTEKCQERISLERLEILGDSFLKFAVARYLFLTHD 1080

Query: 1081 KSDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETRE 1140
            K DEGELTRRRSYLVKN NL KLATRKNLQVYIRDQPFEPSQFY LGRPCPRICNEETR+
Sbjct: 1081 KFDEGELTRRRSYLVKNFNLLKLATRKNLQVYIRDQPFEPSQFYLLGRPCPRICNEETRK 1140

Query: 1141 DIHSHCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGI 1200
            DIHSH +AT N +ASETKCS+GHHWL KKTISDVVEALVGAFLVDSGFKAAI+FLKWIGI
Sbjct: 1141 DIHSHDDAT-NTRASETKCSKGHHWLQKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGI 1200

Query: 1201 QVEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGG 1260
            QVEFE+SLVT+ALM+SN Y++LADR +IS  Q+SLGHQFLHKGLLLQALVHPSYHKHGGG
Sbjct: 1201 QVEFESSLVTDALMASNNYVLLADRTDISAFQDSLGHQFLHKGLLLQALVHPSYHKHGGG 1260

Query: 1261 CYQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLI 1320
            CYQRLEFLGDAVLDYLITSYLYSAYPKLKPG LTDLRSVFVRNEAFANV VDR FYK+L+
Sbjct: 1261 CYQRLEFLGDAVLDYLITSYLYSAYPKLKPGQLTDLRSVFVRNEAFANVTVDRFFYKFLL 1320

Query: 1321 CDSTSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWK 1380
            CDSTSL SDIKSYV+FIK+PS    SL  P CPKALGDLVES VGAVLVDTGFDMNYVWK
Sbjct: 1321 CDSTSLLSDIKSYVDFIKSPSE-RDSLEQPRCPKALGDLVESSVGAVLVDTGFDMNYVWK 1380

Query: 1381 IMLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHATA 1440
            IMLSF+DPI+SFSGFQLSPIRD+IEFCQ+CG  L+FN SK   YYSV+AEVKG NFH TA
Sbjct: 1381 IMLSFVDPIMSFSGFQLSPIRDLIEFCQNCGWKLEFNSSKMEGYYSVKAEVKGGNFHDTA 1440

Query: 1441 SAAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTID 1500
            SAA+RRK+DA+KIAANLILTKLKA+G +PEVNSLEEILKSS +MEPKLIGYDETP   +D
Sbjct: 1441 SAANRRKKDAEKIAANLILTKLKAKGFLPEVNSLEEILKSSSRMEPKLIGYDETPFGMVD 1500

Query: 1501 QADNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFD 1560
            Q DNG  TI G E S ADL+P VH EV N+HP  ITRIS+  VSSS AA EQL PS+A +
Sbjct: 1501 QVDNGLETINGPEFSNADLNPRVHREVDNSHPVHITRISRTPVSSSVAAGEQLKPSMALE 1560

Query: 1561 A--------VSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEI 1620
                      S G   K TARSRLYEICAANHW+ PSFDCMNEEGPSHLKMFTYKVVLE+
Sbjct: 1561 GHDSPADLQSSSGWSGKTTARSRLYEICAANHWNRPSFDCMNEEGPSHLKMFTYKVVLEM 1620

Query: 1621 EEAPNTIFKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYLPETD 1654
            EEAP+TIF+F GAPHLRKKAAAEHAAEAA+WYLKKAGYLPETD
Sbjct: 1621 EEAPDTIFEFVGAPHLRKKAAAEHAAEAALWYLKKAGYLPETD 1650

BLAST of MS002366 vs. NCBI nr
Match: XP_023515970.1 (dicer-like protein 4 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2693.7 bits (6981), Expect = 0.0e+00
Identity = 1385/1672 (82.83%), Postives = 1473/1672 (88.10%), Query Frame = 0

Query: 1    MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPSTRASEKDPRRKARKYQLEL 60
            MPDG+TNS  ADS NFQ TS   VE F+GLS VDDQ  G S R S KDPRR ARKYQLEL
Sbjct: 1    MPDGETNSTTADSVNFQPTSSASVERFMGLSPVDDQGAGSSIRTSGKDPRRIARKYQLEL 60

Query: 61   CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE 120
            C+KALEENII+YLGTGCGKTHIA+LL+YEM HLIRR QKGICVFLAPTVALVQQQAKVIE
Sbjct: 61   CEKALEENIIVYLGTGCGKTHIAILLIYEMGHLIRRPQKGICVFLAPTVALVQQQAKVIE 120

Query: 121  DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFD 180
            DS DFKV+VYCGGSKCLNSHYDWEREMEE+EVFVMTPEILLRNLHHCYIKMEFVELLIFD
Sbjct: 121  DSIDFKVKVYCGGSKCLNSHYDWEREMEEYEVFVMTPEILLRNLHHCYIKMEFVELLIFD 180

Query: 181  ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLS 240
            ECHHAQVKSDHSYAEIMRVFYK N  KRPRIFGMTASPVVGK         GACNQQNLS
Sbjct: 181  ECHHAQVKSDHSYAEIMRVFYKANEHKRPRIFGMTASPVVGK---------GACNQQNLS 240

Query: 241  KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKR 300
            KSINSLEKLLDAKVYSVEDREEL+AFVSSP  KIYYYGPVA G S SFMTYC  LED+K 
Sbjct: 241  KSINSLEKLLDAKVYSVEDREELEAFVSSPRFKIYYYGPVATGSSSSFMTYCCPLEDVKC 300

Query: 301  QCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSEL 360
            QCIV LGQMKGEHE L ATKKLLCRMH NIIFCLENLGVWGALQASKILLS DNSERSEL
Sbjct: 301  QCIVALGQMKGEHEGLLATKKLLCRMHKNIIFCLENLGVWGALQASKILLSEDNSERSEL 360

Query: 361  IEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEIL 420
            IEAAE NP + S+SDRYLNQAAEI A GCKI  G+SD LN DIL+ P FSKKLL+LI IL
Sbjct: 361  IEAAEANPKHDSISDRYLNQAAEIFASGCKIDAGVSDMLNVDILKEPCFSKKLLRLIGIL 420

Query: 421  SSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHI 480
            SSFRHR NMKCIIFVNRIVIARSLSYILQNL+FLA+WKCD+LVGVHSKLRSMSRKTMNHI
Sbjct: 421  SSFRHRPNMKCIIFVNRIVIARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNHI 480

Query: 481  LMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540
            L KFRSGELNLL+ATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV
Sbjct: 481  LTKFRSGELNLLIATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540

Query: 541  DSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLL 600
            DSGNEKELGLIDEF KDE RM+REISSRSS E F+ HEESIYRV STGAS+TSGRSISLL
Sbjct: 541  DSGNEKELGLIDEFHKDEKRMNREISSRSSIETFDGHEESIYRVVSTGASVTSGRSISLL 600

Query: 601  HEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACI 660
            HE+CSKLPHD YFDPKPKFFY DDL GT+CHAILPSNAPI QI+SR QP+   AKKDAC+
Sbjct: 601  HEYCSKLPHDTYFDPKPKFFYCDDLGGTICHAILPSNAPIPQIVSRPQPAGVAAKKDACL 660

Query: 661  KAIEELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSW 720
            KA+EELHKLGAL+D+LL MRD+ANE ELELD SDSD SEDETSR ELHEM+ PAA K+SW
Sbjct: 661  KAVEELHKLGALSDYLLPMRDNANEGELELD-SDSDSSEDETSRRELHEMISPAAFKESW 720

Query: 721  TGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVP 780
            TG GY IL CY IKF+P+P DRIYKEFGLFVKAPLPQEA RMD+ELHLA GRSV VNL+P
Sbjct: 721  TGFGYVILYCYHIKFTPNPEDRIYKEFGLFVKAPLPQEAVRMDLELHLAHGRSVKVNLMP 780

Query: 781  SGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHD 840
            S VVELLE+EITQAE FQEMFLKVILDRSEFV ++IPLRNNVS+SISSTSYLLLP+I HD
Sbjct: 781  SRVVELLENEITQAECFQEMFLKVILDRSEFVHDYIPLRNNVSRSISSTSYLLLPVIVHD 840

Query: 841  NERSVSIDWKVIRRCLFSPIFQIPG-SIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPY 900
            NE SV IDWKVIRRCL S IFQ    SIVKETA S  HL LYDGRRR+SDI+NSLVYVPY
Sbjct: 841  NEGSVFIDWKVIRRCLSSQIFQNHACSIVKETASSDTHLMLYDGRRRSSDIENSLVYVPY 900

Query: 901  KREFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHN 960
            KREFFFVT+IV+ KNGYS+  YK+S SLSHFEHL  RFGIHLKYPEQPLL AKPLF LHN
Sbjct: 901  KREFFFVTNIVRRKNGYSQ--YKDSGSLSHFEHLKTRFGIHLKYPEQPLLCAKPLFSLHN 960

Query: 961  LLHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIE 1020
            LLH+R+Q +SEA YL+EYFID+ PELCQLKI GFSKDIGSSISLLPSIMHRLENLLVAIE
Sbjct: 961  LLHNRKQKDSEAPYLEEYFIDIAPELCQLKITGFSKDIGSSISLLPSIMHRLENLLVAIE 1020

Query: 1021 LKCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDK 1080
            LKCMLAAAFP GAEVTANRILEALTTEKCQERISLERLEILGD FLKFAVARYLFL HDK
Sbjct: 1021 LKCMLAAAFPAGAEVTANRILEALTTEKCQERISLERLEILGDSFLKFAVARYLFLAHDK 1080

Query: 1081 SDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETRED 1140
             DEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFY LGRPCPRICNEETR D
Sbjct: 1081 FDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYLLGRPCPRICNEETRND 1140

Query: 1141 IHSHCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQ 1200
            IHSH NAT NAKASETKCS+GHHWL KKTISDVVEALVGAFLVDSGFKAAI+FLKWIGI+
Sbjct: 1141 IHSHDNATNNAKASETKCSKGHHWLQKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIK 1200

Query: 1201 VEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGC 1260
            VEFEAS VTNAL++S AY++LAD INIS LQ+SLGH+FLHKGLLLQALVHP+YHKHGGGC
Sbjct: 1201 VEFEASQVTNALVASKAYVLLADTINISSLQSSLGHKFLHKGLLLQALVHPTYHKHGGGC 1260

Query: 1261 YQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLIC 1320
            YQRLEFLGDAVLDYLI SYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDR FYK+L+C
Sbjct: 1261 YQRLEFLGDAVLDYLIMSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRFFYKFLLC 1320

Query: 1321 DSTSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKI 1380
            DS SLQSDIKSYV+FIKAP S   SL  P CPK LGDLVES VGAVLVDTGFDMNYVWKI
Sbjct: 1321 DSASLQSDIKSYVDFIKAPPSERDSLEQPRCPKPLGDLVESSVGAVLVDTGFDMNYVWKI 1380

Query: 1381 MLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHATAS 1440
            MLSFLDPI+S SGFQLSPIRDIIE CQ CG  LQFN SK  KYYSVEAEVKGD+FHATAS
Sbjct: 1381 MLSFLDPIMSSSGFQLSPIRDIIEVCQTCGWKLQFNPSKMEKYYSVEAEVKGDDFHATAS 1440

Query: 1441 AAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTIDQ 1500
            A +RRK+DA+KIAAN ILTKLKAQG IPEVNSLE+ILKSS KM+PKLIGYDETPSVT+D+
Sbjct: 1441 AVNRRKKDAEKIAANSILTKLKAQGFIPEVNSLEKILKSSEKMKPKLIGYDETPSVTMDR 1500

Query: 1501 ADNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFD- 1560
             D+      GLE SGADLDP VH EVG++ P RITR+S+ L SSSGAA EQL PS+  + 
Sbjct: 1501 IDD------GLEFSGADLDPRVHREVGDSRPFRITRMSETLASSSGAAGEQLKPSIPLEG 1560

Query: 1561 -----------------AVSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKM 1620
                             + S GR  K TARSRLYE+CA NHW+ PSFDCMNEEGPSHLKM
Sbjct: 1561 HDSSTDLQSLSSSSSSSSSSSGRSGKTTARSRLYEVCAINHWNHPSFDCMNEEGPSHLKM 1620

Query: 1621 FTYKVVLEIEEAPNTIFKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYLPETD 1654
            FTYKVVLEIEEAPNTIF+FFGAPHL KKAAAEHAAEAA+WYLKK  YL +TD
Sbjct: 1621 FTYKVVLEIEEAPNTIFEFFGAPHLTKKAAAEHAAEAALWYLKKERYLGDTD 1654

BLAST of MS002366 vs. ExPASy Swiss-Prot
Match: P84634 (Dicer-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=DCL4 PE=1 SV=2)

HSP 1 Score: 1682.9 bits (4357), Expect = 0.0e+00
Identity = 892/1652 (54.00%), Postives = 1145/1652 (69.31%), Query Frame = 0

Query: 13   SDNFQHTSLGPVESFVG-----LSLVDDQEPGPSTRASEKDPRRKARKYQLELCKKALEE 72
            S +   TS   V SF        S     +P P     EKDPR+ AR+YQ+ELCKKA EE
Sbjct: 82   SSDLHLTSSSSVSSFSSSSSSLFSAAGTDDPSPK---MEKDPRKIARRYQVELCKKATEE 141

Query: 73   NIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIEDSTDFKV 132
            N+I+YLGTGCGKTHIAV+L+YE+ HL+   +K +C+FLAPTVALV+QQAKVI DS +FKV
Sbjct: 142  NVIVYLGTGCGKTHIAVMLIYELGHLVLSPKKSVCIFLAPTVALVEQQAKVIADSVNFKV 201

Query: 133  RVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFDECHHAQV 192
             ++CGG + + SH +WERE+  +EV VMTP+ILL NL HC+IKME + LLIFDECHHAQ 
Sbjct: 202  AIHCGGKRIVKSHSEWEREIAANEVLVMTPQILLHNLQHCFIKMECISLLIFDECHHAQQ 261

Query: 193  KSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLSKSINSLE 252
            +S+H YAEIM+VFYK+   +RPRIFGMTASPVVGK         G+   +NLSKSINSLE
Sbjct: 262  QSNHPYAEIMKVFYKSESLQRPRIFGMTASPVVGK---------GSFQSENLSKSINSLE 321

Query: 253  KLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKRQCIVVLG 312
             LL+AKVYSVE   +LD FVSSPLVK+YYY    +  S S + Y ++LED+K++C+  L 
Sbjct: 322  NLLNAKVYSVESNVQLDGFVSSPLVKVYYYRSALSDASQSTIRYENMLEDIKQRCLASLK 381

Query: 313  QMKGEH--EDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAE 372
             +   H  + L + K+LL R HDN+I+ L NLG+WGA+QA+KI L+ D++ + E +    
Sbjct: 382  LLIDTHQTQTLLSMKRLLKRSHDNLIYTLLNLGLWGAIQAAKIQLNSDHNVQDEPV---- 441

Query: 373  GNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRH 432
            G      + D YL+ AAE L+ G       SD L+   L+ P FS+KL+QLI+ILS FR 
Sbjct: 442  GKNPKSKICDTYLSMAAEALSSGVAKDENASDLLSLAALKEPLFSRKLVQLIKILSVFRL 501

Query: 433  RLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFR 492
              +MKCIIFVNRIV AR+LS IL NLE L  WK D+LVG+ S L+SMSR++M  IL +F+
Sbjct: 502  EPHMKCIIFVNRIVTARTLSCILNNLELLRSWKSDFLVGLSSGLKSMSRRSMETILKRFQ 561

Query: 493  SGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNE 552
            S ELNLLVATKVGEEGLDIQTCCLVIR+DLPETV+SFIQSRGRARMPQSEYAFLVDSGNE
Sbjct: 562  SKELNLLVATKVGEEGLDIQTCCLVIRYDLPETVTSFIQSRGRARMPQSEYAFLVDSGNE 621

Query: 553  KELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCS 612
            KE+ LI+ F+ +E+RM+ EI+ RSS E     +E +Y+V  TGA I+ G SISLL+++CS
Sbjct: 622  KEMDLIENFKVNEDRMNLEITYRSSEETCPRLDEELYKVHETGACISGGSSISLLYKYCS 681

Query: 613  KLPHDEYFDPKPKFFY--YDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAI 672
            +LPHDE+F PKP+F +   D+  GT+C   LP+NAPI +I S   PS E AKKDAC+KA+
Sbjct: 682  RLPHDEFFQPKPEFQFKPVDEFGGTICRITLPANAPISEIESSLLPSTEAAKKDACLKAV 741

Query: 673  EELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDE-TSRGELHEMLVPAALKDSWTG 732
             ELH LG LNDFLL    D  E+EL  D  D D  + E  SRG+L+EM VP   K  W  
Sbjct: 742  HELHNLGVLNDFLLPDSKDEIEDELSDDEFDFDNIKGEGCSRGDLYEMRVPVLFKQKWDP 801

Query: 733  SGYFI-LNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVPS 792
            S   + L+ Y I F PHP DRIYK+FG F+K+PLP EAE MDI+LHLA  RSV V + PS
Sbjct: 802  STSCVNLHSYYIMFVPHPADRIYKKFGFFMKSPLPVEAETMDIDLHLAHQRSVSVKIFPS 861

Query: 793  GVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLR-NNVSKSISSTSYLLLPLIFHD 852
            GV E   DEI  AE FQE+ LKV+ +R E + +F+PL   + S++  ST YLLLPL  HD
Sbjct: 862  GVTEFDNDEIRLAELFQEIALKVLFERGELIPDFVPLELQDSSRTSKSTFYLLLPLCLHD 921

Query: 853  NERSVSIDWKVIRRCLFSPIFQIPGSIVKET-ACSHAHLTLYDGRRRTSDIKNSLVYVPY 912
             E  +S+DW  IR CL SPIF+ P  +V++    S +HL L +G     D+KNSLV+  Y
Sbjct: 922  GESVISVDWVTIRNCLSSPIFKTPSVLVEDIFPPSGSHLKLANGCWNIDDVKNSLVFTTY 981

Query: 913  KREFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHN 972
             ++F+FV DI  G+NG+  S  K SS+ SH E + + +G+ LK+P QPLL  KPL H+ N
Sbjct: 982  SKQFYFVADICHGRNGF--SPVKESSTKSHVESIYKLYGVELKHPAQPLLRVKPLCHVRN 1041

Query: 973  LLHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIE 1032
            LLH+R Q N E   L EYFI++PPEL  LKI G SKDIGSS+SLLPSIMHR+ENLLVAIE
Sbjct: 1042 LLHNRMQTNLEPQELDEYFIEIPPELSHLKIKGLSKDIGSSLSLLPSIMHRMENLLVAIE 1101

Query: 1033 LKCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDK 1092
            LK +L+A+ P+ AEV+ +R+LEALTTEKC ER+SLERLE+LGD FLKFAV+R+LFL HD 
Sbjct: 1102 LKHVLSASIPEIAEVSGHRVLEALTTEKCHERLSLERLEVLGDAFLKFAVSRHLFLHHDS 1161

Query: 1093 SDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETRED 1152
             DEGELTRRRS +V N NL +LA +KNLQVYIRDQ  +P+QF+  G PC   C+E   ++
Sbjct: 1162 LDEGELTRRRSNVVNNSNLCRLAIKKNLQVYIRDQALDPTQFFAFGHPCRVTCDEVASKE 1221

Query: 1153 IHS--HCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIG 1212
            +HS         +   E +CS+GHHWL+KKTI+DVVEALVGAFLVDSGFK A+ FLKWIG
Sbjct: 1222 VHSLNRDLGILESNTGEIRCSKGHHWLYKKTIADVVEALVGAFLVDSGFKGAVKFLKWIG 1281

Query: 1213 IQVEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGG 1272
            + V+FE+  V +A ++S  Y+ L  R N+  L+N L ++FLHKGLL+QA +HPSY++HGG
Sbjct: 1282 VNVDFESLQVQDACIASRRYLPLTTRNNLETLENQLDYKFLHKGLLVQAFIHPSYNRHGG 1341

Query: 1273 GCYQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYL 1332
            GCYQRLEFLGDAVLDYL+TSY ++ +PKLKPG LTDLRS+ V NEA ANVAV  S  ++L
Sbjct: 1342 GCYQRLEFLGDAVLDYLMTSYFFTVFPKLKPGQLTDLRSLSVNNEALANVAVSFSLKRFL 1401

Query: 1333 ICDSTSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVW 1392
             C+S  L   I+ Y NF+ +    SG    P CPK LGDLVESC+GA+ +D GF++N+VW
Sbjct: 1402 FCESIYLHEVIEDYTNFLASSPLASGQSEGPRCPKVLGDLVESCLGALFLDCGFNLNHVW 1461

Query: 1393 KIMLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHAT 1452
             +MLSFLDP+ + S  Q+SPI+++IE CQ    + + + +K    ++VE +V  +    T
Sbjct: 1462 TMMLSFLDPVKNLSNLQISPIKELIELCQSYKWDREISATKKDGAFTVELKVTKNGCCLT 1521

Query: 1453 ASAAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTI 1512
             SA  R KR+  K AA L++T LKA   I   + LE++LK+  + E KLIGY+E P   +
Sbjct: 1522 VSATGRNKREGTKKAAQLMITNLKAHENITTSHPLEDVLKNGIRNEAKLIGYNEDPIDVV 1581

Query: 1513 DQADNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAF 1572
            D        +  LE+ G           GN+  +    I + L  +    +++L P  A 
Sbjct: 1582 DLVGLDVENLNILETFG-----------GNSERSSSYVIRRGLPQAPSKTEDRL-PQKAI 1641

Query: 1573 DAVSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEAPNTI 1632
              +  G     TA+S L+E C AN W PP F+C  EEGP HLK F YKV+LE+E+APN  
Sbjct: 1642 --IKAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMT 1701

Query: 1633 FKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYL 1650
             + +G     KK AAEHAA+AAIW LK +G+L
Sbjct: 1702 LECYGEARATKKGAAEHAAQAAIWCLKHSGFL 1701

BLAST of MS002366 vs. ExPASy Swiss-Prot
Match: A7LFZ6 (Endoribonuclease Dicer homolog 4 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL4 PE=2 SV=1)

HSP 1 Score: 1441.8 bits (3731), Expect = 0.0e+00
Identity = 801/1660 (48.25%), Postives = 1083/1660 (65.24%), Query Frame = 0

Query: 39   GP-STRASEKDPRRKARKYQLELCKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRS 98
            GP STR   KDPR  ARKYQL+LCK+A+EENII+YLGTGCGKTHIAVLL+YE+ HLIR+ 
Sbjct: 15   GPSSTRGEPKDPRTIARKYQLDLCKRAVEENIIVYLGTGCGKTHIAVLLIYELGHLIRKP 74

Query: 99   QKGICVFLAPTVALVQQQAKVIEDSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTP 158
             + +C+FLAPT+ LV+QQA VI  STDFKV+ Y G  K    H +WE +M E EV VMTP
Sbjct: 75   SREVCIFLAPTIPLVRQQAVVIASSTDFKVQCYYGNGKNSRDHQEWENDMREFEVLVMTP 134

Query: 159  EILLRNLHHCYIKMEFVELLIFDECHHAQVKSDHSYAEIMRVFYKTN-VAKRPRIFGMTA 218
            +ILL++L HC+IKM  + LLI DECHHAQ +  H YA+IM+ FY +N V K PR+FGMTA
Sbjct: 135  QILLQSLRHCFIKMNSIALLILDECHHAQPQKRHPYAQIMKEFYNSNSVEKFPRVFGMTA 194

Query: 219  SPVVGK----DHCSTSTN-------------SGACNQQNLSKSINSLEKLLDAKVYSVED 278
            SP++GK     H  T                 G  N+ N +K INSLE+LL AKV SV D
Sbjct: 195  SPIIGKGVMPSHSFTEKGGRSPCQPLIFFLPKGGSNKLNYTKCINSLEELLHAKVCSV-D 254

Query: 279  REELDAFVSSPLVKIYYYGPVANGLSGSFMTYC-SLLEDMKRQCIVVLGQMKGEHEDLRA 338
             EEL++ V+SP +++Y+YGPV +    +  T C   L+ +K Q   +L     + +D + 
Sbjct: 255  NEELESVVASPDMEVYFYGPVNH---SNLTTICIKELDSLKLQSERMLRASLCDFKDSQK 314

Query: 339  TKKLLCRMHDNIIFCLENLGVWGALQASKILLS--GDNSERSELIEAAEGNPNNHSLSDR 398
              K L R+H+NIIFCL+ LG +GALQA++  LS  GD  +R E+    + N +  S +  
Sbjct: 315  KLKSLWRLHENIIFCLQELGSFGALQAARTFLSFDGDKLDRREV----DLNGSTSSFAHH 374

Query: 399  YLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCIIFVN 458
            YLN A  IL++  K  G  + + + + LE PFFS K   LI +LS +  + NMKCI+FV 
Sbjct: 375  YLNGATSILSRN-KTDGSHAGSFDLEKLEEPFFSNKFSVLINVLSRYGLQENMKCIVFVK 434

Query: 459  RIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLLVATK 518
            RI +AR++S ILQNL+ L FWKC++LVG HS  ++MSR  M+ I+ +F SGE+NLLVAT 
Sbjct: 435  RITVARAISNILQNLKCLEFWKCEFLVGCHSGSKNMSRNKMDAIVQRFSSGEVNLLVATS 494

Query: 519  VGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLIDEFQK 578
            VGEEGLDIQTCCLV+RFDLPETV+SFIQSRGRARM +S+Y  L++  N+    L++ +  
Sbjct: 495  VGEEGLDIQTCCLVVRFDLPETVASFIQSRGRARMTKSKYVVLLERENQSHEKLLNGYIA 554

Query: 579  DENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEYFDPK 638
             E+ M+ EI SR+S+++F+  EE+IY+V +TGASI++  S+SLLH +C  LP D +F P 
Sbjct: 555  GESIMNEEIDSRTSNDMFDCLEENIYQVDNTGASISTACSVSLLHCYCDNLPRDMFFTPS 614

Query: 639  PKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGALNDFL 698
            P FFY D + G +C  ILP NA   Q   +   S++ AK+DAC+KA  +LHKLGAL DFL
Sbjct: 615  PVFFYIDGIEGIICRLILPPNAAFRQADGQPCLSKDEAKRDACLKACVKLHKLGALTDFL 674

Query: 699  LLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSWTG-SGYFILNCYQIKF 758
            L        +    ++S +++ ED++ R ELHEML+PA LK S         L+ Y +KF
Sbjct: 675  LPGPGSRKNKVSVTNNSSNNKVEDDSLREELHEMLIPAVLKPSGLKLDSLSNLHFYYVKF 734

Query: 759  SPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEITQAE 818
             P P DR Y+ FGLFV  PLP EAE + ++LHLARGR V   +   G +   ++++  A 
Sbjct: 735  IPIPEDRRYQMFGLFVINPLPVEAETLQVDLHLARGRIVKAGIKHLGKIAFEKEKMMLAH 794

Query: 819  SFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHDNERSVSIDWKVIRRC 878
             FQEM LK++LDRSEF    + L N+V+  I+ST YLLLP+          IDW  + RC
Sbjct: 795  KFQEMCLKILLDRSEFTSPHVKLGNDVTLEINSTFYLLLPIKQKCYGDRFMIDWPAVERC 854

Query: 879  LFSPIFQIPGSI-VKETACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKGKN 938
            L SPIF+ P  + V  +  S+  L L DG    +D+  S+V+ P+   FFFV  I+   N
Sbjct: 855  LSSPIFKDPIDVSVHASYSSNESLRLLDGIFSKTDVVGSVVFSPHNNIFFFVDGILDEIN 914

Query: 939  GYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDR--RQGNSEAC 998
             +S       S  ++ EH  ERF I L +PEQPLL AK +F+L NLLH+R      SE  
Sbjct: 915  AWS-----EHSGATYAEHFKERFRIELSHPEQPLLKAKQIFNLRNLLHNRLPETTESEGR 974

Query: 999  YLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGA 1058
             L E+F++LPPELC LK+IGFSKD+GSS+SLLPS+M+RLENLLVAIELK ++ ++FP+ +
Sbjct: 975  ELLEHFVELPPELCSLKVIGFSKDMGSSLSLLPSLMYRLENLLVAIELKDVMLSSFPEAS 1034

Query: 1059 EVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYL 1118
            +++A+ ILEALTTEKC ERISLER E+LGD FLK+ V R+ F+T++  DEG+LTRRRS +
Sbjct: 1035 QISASGILEALTTEKCLERISLERFEVLGDAFLKYVVGRHKFITYEGLDEGQLTRRRSDV 1094

Query: 1119 VKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDIHSHCNATKNAKA 1178
            V N +L++L+ RK LQVYIRDQ FEP+QF+  GRPC  +CN +    +H       N + 
Sbjct: 1095 VNNSHLYELSIRKKLQVYIRDQQFEPTQFFAPGRPCKVVCNTDVEVRLHQMDIHPDNREN 1154

Query: 1179 SETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALM 1238
               +C+R HHWLH+K I+DVVE+L+GAFLV+ GFKAA +FL WIGI V+F    +   L 
Sbjct: 1155 CNLRCTRSHHWLHRKVIADVVESLIGAFLVEGGFKAAFAFLHWIGIDVDFNNPALYRVLD 1214

Query: 1239 SSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCYQRLEFLGDAVLD 1298
            SS+  + L D  +I+ L+  +G++F HKGLLLQA VHPS+ +H GGCYQRLEFLGDAVL+
Sbjct: 1215 SSSINLSLMDYTDIAGLEELIGYKFKHKGLLLQAFVHPSFSQHSGGCYQRLEFLGDAVLE 1274

Query: 1299 YLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYV 1358
            Y+ITSYLYS YP +KPG +TDLRS+ V N++ A  AV++S +K+LI DS  L S I  + 
Sbjct: 1275 YVITSYLYSTYPDIKPGQITDLRSLAVGNDSLAYAAVEKSIHKHLIKDSNHLTSAISKFE 1334

Query: 1359 NFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSG 1418
             ++K  +S    L  P+CPKALGD+VESC+GAVL+D+GF++NYVWK+ML  L P+++F+ 
Sbjct: 1335 MYVKLSNSEKDLLEEPACPKALGDIVESCIGAVLLDSGFNLNYVWKVMLMLLKPVLTFAN 1394

Query: 1419 FQLSPIRDIIEFCQDCGSNLQF-NESKTGKYYSVEAEVKGDNFHATASAAHRRKRDAKKI 1478
               +P+R++ E CQ  G  L      K    Y V+ EV   +     +AA+R  + A+K 
Sbjct: 1395 MHTNPMRELRELCQCHGFELGLPKPMKADGEYHVKVEVNIKSKIIICTAANRNSKAARKF 1454

Query: 1479 AANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTIDQADNGFGTIKGLE 1538
            AA   L+KLK  G      SLEEIL  +RK E +LIGY+E P   ID   +    +K   
Sbjct: 1455 AAQETLSKLKNYGYKHRNKSLEEILIVARKRESELIGYNEDP---IDVEADISVKMKSPH 1514

Query: 1539 SSGADLDPFVHSEVGNAHPARITRISKMLVSSSG--------------AAQEQLDPSLAF 1598
                ++ PF ++E       R ++    +++ SG              A Q    PS A 
Sbjct: 1515 IHEENI-PFQNTETSF---TRSSKFHNQIIAGSGKHDVNNGRNNQPKLATQSGRLPSEAT 1574

Query: 1599 D----AVSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEA 1654
            +     V  G +   TARS L+E+CAAN+W PP F    EEGPSHL+ FTYKVV+EI+ A
Sbjct: 1575 EKSNKKVYHGDMVHKTARSFLFELCAANYWKPPEFKLCKEEGPSHLRKFTYKVVVEIKGA 1634

BLAST of MS002366 vs. ExPASy Swiss-Prot
Match: Q5N870 (Endoribonuclease Dicer homolog 3a OS=Oryza sativa subsp. japonica OX=39947 GN=DCL3A PE=2 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 1.0e-203
Identity = 525/1681 (31.23%), Postives = 832/1681 (49.49%), Query Frame = 0

Query: 54   RKYQLELCKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKG-ICVFLAPTVALV 113
            R+YQL++ + A+  N I  L TG GKT IAV+L+ E   + R    G + +FLAPTV LV
Sbjct: 33   RRYQLDVYEVAMRRNTIAMLDTGAGKTMIAVMLIKEFGKINRTKNAGKVIIFLAPTVQLV 92

Query: 114  QQQAKVIEDSTDFKVRVYCGGSKCLN-SHYDWEREMEEHEVFVMTPEILLRNLHHCYIKM 173
             QQ +VIE  TDF+V  Y G       +   W+ ++ +++V VMTP++ L+ L + ++ +
Sbjct: 93   TQQCEVIEIHTDFEVEQYYGAKGVDQWTGPRWQEQISKYQVMVMTPQVFLQALRNAFLIL 152

Query: 174  EFVELLIFDECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNS 233
            + V L+IFDECHHA    +H Y  IM+ FY  +  K P +FGMTASPV+ K         
Sbjct: 153  DMVSLMIFDECHHA--TGNHPYTRIMKEFYHKSEHK-PSVFGMTASPVIRK--------- 212

Query: 234  GACNQQNLSKSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYG--PVA-NGLSGSF 293
            G  +  +       LE LLDAK+Y+V DREE++  V S      YY   PV    LS   
Sbjct: 213  GISSHLDCEGQFCELENLLDAKIYTVSDREEIEFCVPSAKEMCRYYDSKPVCFEDLSEEL 272

Query: 294  MTYCSLLEDMKRQCIVVLGQMKGEHEDL-RATKKLLCRMHDNIIFCLENLGVWGALQASK 353
               CS  + +  +       M  + +D+ + +K+ L +    I +CL+++G+  A +A+K
Sbjct: 273  GVLCSKYDALITELQNKRSDMYKDADDITKESKRRLSKSIAKICYCLDDVGLICASEATK 332

Query: 354  ILLSGDNSE--RSELIEAAEGNPN---------NHSLSDRYLNQAAEILAKGCKIGGGM- 413
            I +     +    E+++A +   +         N +L  ++  +A  ++ K  + G  M 
Sbjct: 333  ICIERGQEKGWLKEVVDATDQQTDANGSRLFAENSALHMKFFEEALHLIDKRLQQGIDML 392

Query: 414  --SDTLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCIIFVNRIVIARSLSYILQNLEF 473
              S++   +  +  + S KL +LI+I  SF +  + +C+IFV+R + AR +  +++ +  
Sbjct: 393  LNSESGCVEAAKTGYISPKLYELIQIFHSFSNSRHARCLIFVDRKITARVIDRMIKKIGH 452

Query: 474  LAFWKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLLVATKVGEEGLDIQTCCLVIRF 533
            LA +   +L G  S + +++ K     L  FRSG++NLL  T V EEG+ +  C  VIRF
Sbjct: 453  LAHFTVSFLTGGRSSVDALTPKMQKDTLDSFRSGKVNLLFTTDVAEEGIHVPECSCVIRF 512

Query: 534  DLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLIDEFQKDENRMDREISSRSSSEI 593
            DLP T  S++QSRGRAR   S+Y  +++ GN K+  LI    + E  M +  SSR S  +
Sbjct: 513  DLPRTTRSYVQSRGRARQEDSQYILMIERGNVKQNDLISAIVRSETSMVKIASSRESGNL 572

Query: 594  ---FNSHEE-SIYRVASTGASITSGRSISLLHEFCSKLPHDEYFDPKPKFFYYDDLAGTV 653
               F  +EE + Y V +TGA +T+  SIS+++ +C KLP D+ + PKP F +     G V
Sbjct: 573  SPGFVPNEEINEYHVGTTGAKVTADSSISIVYRYCEKLPQDKCYSPKPTFEFTHHDDGYV 632

Query: 654  CHAILPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGALNDFLLLMRDDANEEELE 713
            C   LP +A +  ++     +   AK+  C+ A ++LH+LGAL+D L L  +D      E
Sbjct: 633  CTLALPPSAVLQILVGPKARNMHKAKQLVCLDACKKLHELGALDDHLCLSVEDPVP---E 692

Query: 714  LDSSDSDRSEDETSRGELHEMLVPAALKDSWTGSGYFI-LNCYQIKFSPHPRDRIYKEFG 773
            + S +       T R ELH      A   +W      + L  Y++ F      +IY EF 
Sbjct: 693  IVSKNKGTGIGTTKRKELHGTTRIHAWSGNWVSKKTALKLQSYKMNFVCDQAGQIYSEFV 752

Query: 774  LFVKAPLPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEITQAESFQEMFLKVILDR 833
            L + A LP E   ++I+L+L   + V  ++   G++EL   ++ QA+ FQ +    +  +
Sbjct: 753  LLIDATLPDEVATLEIDLYL-HDKMVKTSVSSCGLLELDAQQMEQAKLFQGLLFNGLFGK 812

Query: 834  -------SEFVQEFIPLRNNVSKSISSTSYLLLPLIFHDNERSVSIDWKVIRRCLFSPIF 893
                       +EFI  + +     +++ YLLLP      + +V I+W VI     +   
Sbjct: 813  LFTRSKVPNAPREFILNKEDTFVWNTASVYLLLP-TNPSFDSNVCINWSVIDAA--ATAV 872

Query: 894  QIPGSIVKETACSHAHLTLYDGRRRTSD-------------IKNSLVYVPYKREFFFVTD 953
            ++   I  E       L ++D  +   D             +K+ +V   +  + +   D
Sbjct: 873  KLMRRIYSEN--KRELLGIFDSDQNVGDLIHLANKSCKANSLKDMVVLAVHTGKIYTALD 932

Query: 954  I--VKGKNGYSRSHYKNSSSLSHF-EHLIERFGIHLKYPEQPLLSAKPLFHLHNLLHD-- 1013
            I  + G + +  +  K       F E+  +++GI L++P QPLL  KP  + HNLL    
Sbjct: 933  ITELSGDSAFDGASDKKECKFRTFAEYFKKKYGIVLRHPSQPLLVLKPSHNPHNLLSSKF 992

Query: 1014 RRQGN--------SEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLL 1073
            R +GN        +         + +PPEL  L  +    +I  S  L P++M+R+E+L 
Sbjct: 993  RDEGNVVENMSNGTPVVNKTSNRVHMPPEL--LIPLDLPVEILRSFYLFPALMYRIESLT 1052

Query: 1074 VAIELKCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFL 1133
            +A +L+  +  +    + +++  ILEA+TT +C E  S+ERLE+LGD  LK+AV+ +LFL
Sbjct: 1053 LASQLRSEIGYS---DSNISSFLILEAITTLRCSEDFSMERLELLGDSVLKYAVSCHLFL 1112

Query: 1134 THDKSDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLG----RPCPRI 1193
                 DEG+L+  R +++ N  L+KL   +N+Q Y+RD  F+P ++   G    RP P  
Sbjct: 1113 KFPNKDEGQLSSIRCHMICNATLYKLGIERNVQGYVRDAAFDPRRWLAPGQLSIRPSPCE 1172

Query: 1194 C---NEETREDIHSHCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKA 1253
            C   +E   +DIH        A      C +GH W+  KTI+D VEA++GA+    G +A
Sbjct: 1173 CPVKSEVVTDDIHI---IDDKAIVLGKACDKGHRWMCSKTIADCVEAIIGAYYAGGGLRA 1232

Query: 1254 AISFLKWIGIQVEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALV 1313
            A++ LKW+GI  E E  L+  A++S++    L       +L+  LG+ F  KGLL++AL 
Sbjct: 1233 AMAVLKWLGIGAEIEEDLIVQAILSASVQTYLPKDNVFEMLEAKLGYSFSVKGLLVEALT 1292

Query: 1314 HPSYHKHGGG-CYQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANV 1373
            HPS  + G   CY+RLEFLGDAVLD L+T YL++++     G LTDLRS  V NE FA V
Sbjct: 1293 HPSQQELGAKYCYERLEFLGDAVLDILLTRYLFNSHKDTNEGELTDLRSASVNNENFAQV 1352

Query: 1374 AVDRSFYKYLICDSTSLQSDIKSYVNFIKAPSSGSGSL---GLPSCPKALGDLVESCVGA 1433
            AV  +F+ +L   S  L   I  YVN ++  S     L   GLP  PK LGD+VES  GA
Sbjct: 1353 AVKHNFHHFLQHSSGLLLDQITEYVNRLEGSSMDKVELLSDGLPKGPKVLGDIVESIAGA 1412

Query: 1434 VLVDTGFDMNYVWKIMLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYS 1493
            +L+DT  D++ VW I    L PI++    +L P R++IE+C   G  +  N    G    
Sbjct: 1413 ILLDTKLDLDVVWGIFEPLLSPIVTPENLELPPYRELIEWCGKHGYFVGINCRDQGDTVV 1472

Query: 1494 VEAEVKGDNFHATASAAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEP 1553
               +V+            ++++DAK  A++L+L  L+ +GLI        I K++ K E 
Sbjct: 1473 ATLDVQLKEVLLVRQGFSKKRKDAKAHASSLLLKDLEEKGLI--------IPKNASKTEQ 1532

Query: 1554 KLIGYDETPSVTIDQADNGFGTIKGLESSGADLDPFVHSEVGNAH-PARITRISKMLVSS 1613
                                      E      +PF      N H  A  T+  K     
Sbjct: 1533 -------------------------FEKHCGSTNPF-----NNLHVDAMDTQTPKPTKEK 1592

Query: 1614 SGAAQEQL-DPSLAFDAVSLGRLCKATARSRLYEICAANHWSPPSFD-----------CM 1650
            + A    + DP L      + +  K   R  LYE+C    W  P+ +           C 
Sbjct: 1593 NAADSRNISDPLLGKPLHVIVKTSKGGPRIALYELCKKLQWPMPTMESEKVQPSFSSVCS 1646

BLAST of MS002366 vs. ExPASy Swiss-Prot
Match: Q3EBC8 (Endoribonuclease Dicer homolog 2 OS=Arabidopsis thaliana OX=3702 GN=At3g03300 PE=1 SV=2)

HSP 1 Score: 661.8 bits (1706), Expect = 2.1e-188
Identity = 472/1435 (32.89%), Postives = 746/1435 (51.99%), Query Frame = 0

Query: 49   PRRKARKYQLELCKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPT 108
            P   AR YQ+E  +KA+++N I++L TG GKT IA++L+    +L R+     CVFL P 
Sbjct: 20   PLHFARSYQVEALEKAIKQNTIVFLETGSGKTLIAIMLLRSYAYLFRKPSPCFCVFLVPQ 79

Query: 109  VALVQQQAKVIEDSTDFKVRVYCG--GSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHH 168
            V LV QQA+ ++  TD KV +Y G  G    +S   W++E++++EV VMTP ILL  L H
Sbjct: 80   VVLVTQQAEALKMHTDLKVGMYWGDMGVDFWDSS-TWKQEVDKYEVLVMTPAILLDALRH 139

Query: 169  CYIKMEFVELLIFDECHHAQVKSDHSYAEIMRVFY----KTNVAKRPRIFGMTASPVVGK 228
             ++ +  +++LI DECHHA  K  H YA IMR FY     +  +  PRIFGMTAS V  K
Sbjct: 140  SFLSLSMIKVLIVDECHHAGGK--HPYACIMREFYHKELNSGTSNVPRIFGMTASLVKTK 199

Query: 229  DHCSTSTNSGACNQQNLSKSINSLEKLLDAKVYSVEDREELDAFV--SSPLVKIYYYGPV 288
                        N  +  K I+ LE L+++KVY+ E+   L  FV  S+P  K Y +  +
Sbjct: 200  GE----------NLDSYWKKIHELETLMNSKVYTCENESVLAGFVPFSTPSFKYYQHIKI 259

Query: 289  ANGLSGSFMTYCSLLEDMKRQCI---VVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENL 348
             +          SL+E ++R  I   + LG +      + + +K L R+   + +CL++L
Sbjct: 260  PS------PKRASLVEKLERLTIKHRLSLGTLDLNSSTVDSVEKRLLRISSTLTYCLDDL 319

Query: 349  GVWGALQASKILLSGDNSERSELIEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSD 408
            G+  A +A++ L +  N    +     E N  + +L  ++ + A++        G   S 
Sbjct: 320  GILLAQKAAQSLSASQN----DSFLWGELNMFSVALVKKFCSDASQEFLAEIPQGLNWSV 379

Query: 409  TLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCIIFVNRIVIARSL-SYILQNLEFLAF 468
                   E    + K + LIE L  +    N++CIIFV+R++ A  L S + + L     
Sbjct: 380  ANINGNAEAGLLTLKTVCLIETLLGYSSLENIRCIIFVDRVITAIVLESLLAEILPNCNN 439

Query: 469  WKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLP 528
            WK  Y+ G +S L++ +RK  N I+  FR G +N++VAT + EEGLD+Q+C LVIRFD  
Sbjct: 440  WKTKYVAGNNSGLQNQTRKKQNEIVEDFRRGLVNIIVATSILEEGLDVQSCNLVIRFDPA 499

Query: 529  ETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLIDEFQKDENRMDREISSRS---SSEI 588
              + SFIQSRGRARM  S+Y  +V+SG+      + ++     RM  E    S      +
Sbjct: 500  SNICSFIQSRGRARMQNSDYLMMVESGDLLTQSRLMKYLSGGKRMREESLDHSLVPCPPL 559

Query: 589  FNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAI 648
             +  +E ++RV STGA++T   S+SL++ +CS+LP DEYF P P+F    D     C   
Sbjct: 560  PDDSDEPLFRVESTGATVTLSSSVSLIYHYCSRLPSDEYFKPAPRFDVNKDQGS--CTLY 619

Query: 649  LPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGALNDFLLLMRDDANEEELELDSS 708
            LP + P+ ++  + + + +  K+  C+KA  +LHK+GAL+D L+         ++ +  +
Sbjct: 620  LPKSCPVKEV--KAEANNKVLKQAVCLKACIQLHKVGALSDHLV--------PDMVVAET 679

Query: 709  DSDRSEDETSRGELHEMLVPAALKDSWTGSGYFILNCYQIKFSPH-PRDRIYKEFGLFVK 768
             S + E      E      P  L   ++       + Y I+  P+ PR+    +  L  +
Sbjct: 680  VSQKLEKIQYNTE-QPCYFPPELVSQFSAQPETTYHFYLIRMKPNSPRNFHLNDVLLGTR 739

Query: 769  APLPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEITQAESFQEMFLKVILDRSEFV 828
              L  +       L   RG ++ V L   G   L ++E+     FQ    +V+LD S  V
Sbjct: 740  VVLEDDIGNTSFRLEDHRG-TIAVTLSYVGAFHLTQEEVLFCRRFQITLFRVLLDHS--V 799

Query: 829  QEFIPLRNNVS-KSISSTSYLLLPLIFHDNERSVSIDWKVIRRCLFSPIFQIPGSIVKET 888
            +  +   N +  +   +  YLL+P   H +E S+ IDW+VIR    +    +       T
Sbjct: 800  ENLMEALNGLHLRDGVALDYLLVPST-HSHETSL-IDWEVIRSVNLTSHEVLEKHENCST 859

Query: 889  ACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKGKNGYSRSHYKNSSSLSHFE 948
              +   L   DG   T  ++N+LVY P+    +    ++   NG S    +NS   ++ E
Sbjct: 860  NGASRILHTKDGLFCTCVVQNALVYTPHNGYVYCTKGVLNNLNGNSLLTKRNSGDQTYIE 919

Query: 949  HLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEACYLQEYFIDLPPELCQLKII 1008
            +  ER GI L + ++PLL+ + +F LH+ LH  ++   +       F++LPPELC + + 
Sbjct: 920  YYEERHGIQLNFVDEPLLNGRHIFTLHSYLHMAKKKKEKE--HDREFVELPPELCHVILS 979

Query: 1009 GFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGAEVTANRILEALTTEKCQER 1068
              S D+  S + +PS+M R+E+LL+A  LK  +         +   ++LEA+TT+KC+++
Sbjct: 980  PISVDMIYSYTFIPSVMQRIESLLIAYNLKKSIPK-----VNIPTIKVLEAITTKKCEDQ 1039

Query: 1069 ISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYLVKNINLFKLATRKNLQVYI 1128
              LE LE LGD FLK+AV + LF       EG L+ ++  ++ N+ L +   ++ LQ +I
Sbjct: 1040 FHLESLETLGDSFLKYAVCQQLFQHCHTHHEGLLSTKKDGMISNVMLCQFGCQQKLQGFI 1099

Query: 1129 RDQPFEPSQFYTLGRPCP--RICNEETREDIHSHCNATKNAKASETKCSRGHHWLHKKTI 1188
            RD+ FEP  +   G+      + N+   E  + +  + +N K              +K++
Sbjct: 1100 RDECFEPKGWMVPGQSSAAYSLVNDTLPESRNIYVASRRNLK--------------RKSV 1159

Query: 1189 SDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALMSSNAYIILADRINISVL 1248
            +DVVE+L+GA+L + G  AA+ F+ W+GI+V+F     T   +  ++ I     +N+  +
Sbjct: 1160 ADVVESLIGAYLSEGGELAALMFMNWVGIKVDF-----TTTKIQRDSPIQAEKLVNVGYM 1219

Query: 1249 QNSLGHQFLHKGLLLQALVHPSY-HKHGGGCYQRLEFLGDAVLDYLITSYLYSAYPKLKP 1308
            ++ L + F  K LL++AL H SY       CYQRLEFLGD+VLDYLIT +LY  YP L P
Sbjct: 1220 ESLLNYSFEDKSLLVEALTHGSYMMPEIPRCYQRLEFLGDSVLDYLITKHLYDKYPCLSP 1279

Query: 1309 GLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYVNFIKAPS--SGSGSLG 1368
            GLLTD+RS  V NE +A VAV  + +K+++  S  L   I   V+  +  S  S  G   
Sbjct: 1280 GLLTDMRSASVNNECYALVAVKANLHKHILYASHHLHKHISRTVSEFEQSSLQSTFGWES 1339

Query: 1369 LPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSGFQLSPIRDIIEFCQ 1428
              S PK LGD++ES  GA+ VD+G++   V+  +   L  +I+    +L P+R++ E CQ
Sbjct: 1340 DISFPKVLGDVIESLAGAIFVDSGYNKEVVFASIKPLLGCMITPETVKLHPVRELTELCQ 1383

Query: 1429 DCGSNLQFNESKTGKYYSVEAEVKGDNFHATASAAHRRKRDAKKIAANLILTKLK 1462
                  QF  SK   + S   EVK        +A    K+ AKK+A   +L  LK
Sbjct: 1400 ----KWQFELSKAKDFDSFTVEVKAKEMSFAHTAKASDKKMAKKLAYKEVLNLLK 1383

BLAST of MS002366 vs. ExPASy Swiss-Prot
Match: Q7XD96 (Endoribonuclease Dicer homolog 3b OS=Oryza sativa subsp. japonica OX=39947 GN=DCL3B PE=3 SV=2)

HSP 1 Score: 639.0 bits (1647), Expect = 1.4e-181
Identity = 518/1651 (31.37%), Postives = 791/1651 (47.91%), Query Frame = 0

Query: 58   LELCKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQ--KGICVFLAPTVALVQQQ 117
            +E+ + AL  N I  L TG GKT +AV+L  E    +R  +  + I VFLAPTV LV QQ
Sbjct: 44   VEVFEAALRGNTIAVLDTGSGKTMVAVMLAREHARRVRAGEAPRRIVVFLAPTVHLVHQQ 103

Query: 118  AKVIEDSTDFKVRVYCGGSKCLNSHYD-WEREMEEHEVFVMTPEILLRNLHHCYIKMEFV 177
             +VI + TD  V +  G S+      D W+ E+  +E+ VMTP+ILL  L H ++ M  V
Sbjct: 104  FEVIREYTDLDVMMCSGASRVGEWGADHWKEEVGRNEIVVMTPQILLDALRHAFLTMSAV 163

Query: 178  ELLIFDECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGAC 237
             LLIFDECH A     H YA IM+ FY      RP +FGMTASPV  K         GA 
Sbjct: 164  SLLIFDECHRA--CGSHPYARIMKEFY-FGSQWRPDVFGMTASPVATK---------GAS 223

Query: 238  NQQNLSKSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSL 297
               N    I+ LE  LDAK+Y VEDR EL++F     +   YY         +  +   +
Sbjct: 224  TLHNCEAHISQLELTLDAKIYIVEDRNELESFSPPTTIVNKYYDAYMVDFD-NLKSKLQI 283

Query: 298  LEDMKRQCIVVL-----GQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKIL 357
              D     +V L      + K     L  ++K L R H  I++ L +LG     +  KI 
Sbjct: 284  FSDEFDSLLVGLQESPSNKFKDTDNILETSRKSLSRYHGKILYSLNDLGPIITSEVVKIH 343

Query: 358  LSGDN--SERSELIEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGM-SDTLNADILEY 417
            +       +  + I +      + S     L+   EIL +G   G  M S++ + ++ + 
Sbjct: 344  IESVKPLCDSEDCIFSKASLCLHMSYFKEALSLIEEILPQG--YGELMKSESGSEELTKR 403

Query: 418  PFFSKKLLQLIEILSSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVH 477
             + S K+  LI I  SF     + C+IFV+RI+ A+++   ++ +   + +   YL G  
Sbjct: 404  GYISSKVNTLINIFKSFGSSNEVLCLIFVDRIITAKAVERFMRGIVNFSCFSISYLTGGS 463

Query: 478  SKLRSMSRKTMNHILMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSR 537
            +   ++S       L  FR+G++NLL  T V EEG+D+  C  VIRFDLP TV S++QSR
Sbjct: 464  TSKDALSPAVQRFTLDLFRAGKVNLLFTTDVTEEGVDVPNCSCVIRFDLPRTVCSYVQSR 523

Query: 538  GRARMPQSEYAFLVDSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESI----- 597
            GRAR   SE+  +++ GN ++   I    +    +      R  + +  S++ SI     
Sbjct: 524  GRARRNNSEFILMIERGNLQQQEHIFRMIQTGYYVKNCALYRHPNAL--SYDLSIQGMYT 583

Query: 598  YRVASTGASITSGRSISLLHEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIH 657
            Y+V STGA+IT+   ++L+ ++C KLP D YF PKP F    +     C   LP NA   
Sbjct: 584  YQVQSTGATITADCCVNLIRKYCEKLPKDRYFMPKPSFEVTIEDGLFKCTLTLPRNAAFQ 643

Query: 658  QIISRTQPSREGAKKDACIKAIEELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDE 717
             I+     S   +K+   ++A ++LH+LG LND L+ + ++  + +              
Sbjct: 644  SIVGPLSSSSNLSKQLVSLEACKKLHQLGELNDHLVPLTEEPMDTDFTTADEKCISGPGT 703

Query: 718  TSRGELHEMLVPAALKDSWT-GSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAE 777
            T R ELH      AL  +W   S    LN Y+I F        Y  F L ++  L  +  
Sbjct: 704  TKRKELHGTTCVLALSGTWIHDSENITLNTYRIDFLCDQEGENYAGFVLLMEPELDDDVA 763

Query: 778  RMDIELHLARGRSVMVNLVPSGVVELLEDEITQAESFQEMFLKVILDR-------SEFVQ 837
               ++L L   + V   + P G V+L + ++ + + FQE F   I  R       S   +
Sbjct: 764  PSKMDLFLIPNKMVYTTVTPRGKVQLNKKQLGKGKLFQEFFFNGIFGRLFHGSRKSGAQR 823

Query: 838  EFIPLRNNVSKSISSTSYLLLPLIFHDN---ERSVSIDWKVIRRC------LFS------ 897
            +FI  + +  +  + + YLLLPL   D+   +  +SI W+ I  C      L+S      
Sbjct: 824  DFIFKKGHEIQWNTESMYLLLPL--RDSSYIQDDLSIHWEAIESCAGAVEQLWSSYQGDE 883

Query: 898  ---PIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIV---KG 957
               P+  IP    K        + L +     S IK+S+V   +    + V D++     
Sbjct: 884  NVIPVNCIP---QKRRGGQEEIIHLANKSLHCSSIKDSVVLSLHTGRIYTVLDLILDTTA 943

Query: 958  KNGYSRSHYKNSSSLSHF-EHLIERFGIHLKYPEQPLLSAKPLFHLHNLLH------DRR 1017
            ++ +       +S  + F ++  +++GI +++PEQPLL  K   + HNLL       D  
Sbjct: 944  EDSFDEMCKGKASPFTSFVDYYHQKYGIIIQHPEQPLLLLKQSHNAHNLLFSKLKYLDGS 1003

Query: 1018 QGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLA 1077
             G       ++    +PPEL  L  +  + DI  S  LLPS++HRL++L++A +L+  + 
Sbjct: 1004 TGKPLLMEKEQIHARVPPEL--LIHLDVTTDILKSFYLLPSVIHRLQSLMLASQLRREIG 1063

Query: 1078 AAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKSDEGEL 1137
                    +    ILEA+TT +C E  SLERLE+LGD  LK+ V   LFL +    EG+L
Sbjct: 1064 Y----NQHIPVTLILEAITTLRCCETFSLERLELLGDSVLKYVVGCDLFLRYPMKHEGQL 1123

Query: 1138 TRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLG----RPCPRICNEETREDIH 1197
            +  RS  V N  L K    ++LQ Y+RD  F+P ++   G    RP P  C  ET   + 
Sbjct: 1124 SDMRSKAVCNATLHKHGIWRSLQGYVRDNAFDPRRWVAPGQISLRPFPCNCGIET-AFVP 1183

Query: 1198 SHCNATKNAKA--SETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQ 1257
            SH    ++  +      C RGH W+  KTISD VEALVGA+ V  G  AA+  ++W GI 
Sbjct: 1184 SHRRYIRDDPSFFVGKPCDRGHRWMCSKTISDCVEALVGAYYVGGGIAAALWVMRWFGID 1243

Query: 1258 VEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHG-GG 1317
            ++ +  L+     +++    L+   +I  L+  L + F  KGLLL+A+ HPS  + G   
Sbjct: 1244 IKCDMKLLQEVKFNASHLCSLSKINDIEELEAKLKYNFSVKGLLLEAITHPSLQELGVDY 1303

Query: 1318 CYQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLI 1377
            CYQRLEFLGD+VLD L+T +LY+ +  + PG LTDLRS  V NE FA   V  + + +L 
Sbjct: 1304 CYQRLEFLGDSVLDLLLTRHLYATHTDVDPGELTDLRSALVSNENFAQAVVRNNIHSHLQ 1363

Query: 1378 CDSTSLQSDIKSYV-NFIKAPSSGSGSLGLPSC--PKALGDLVESCVGAVLVDTGFDMNY 1437
              S  L   I  YV + ++     S  L   +C  PK LGD++ES  GAV +DT F+++ 
Sbjct: 1364 HGSGILLEQITEYVRSNLECQGKESEFLQHTTCKVPKVLGDIMESIAGAVFIDTDFNVDM 1423

Query: 1438 VWKIMLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFH 1497
            VW+I    L P+I+     L P R+++E C   G  L    +  G+   +E  ++  +  
Sbjct: 1424 VWEIFEPLLSPLITPDKLALPPYRELLELCSHIGCFLNSKCTSKGEEVIIEMSLQLRDEL 1483

Query: 1498 ATASAAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSV 1557
              A    R K+ AK  AA+ IL  LK QGL     S+++ L  +++++            
Sbjct: 1484 LVAQGHDRNKKRAKAKAASRILADLKQQGL-----SIKQCLSKAKQLD------------ 1543

Query: 1558 TIDQADNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSL 1617
             I  +D  F     L SSG  L    +S++ + H         +L   S   +E + P  
Sbjct: 1544 -IVTSDLQF----DLTSSGTQLS---YSDLNDYH---------ILEGLSSVKKEVVLPL- 1603

Query: 1618 AFDAVSLGRLCKATARSRLYEICAANHWSPPSFD----------CMNEEGPSHLKMFTYK 1637
                    ++ K   RS L+++C    W  P F+           M+    ++   F   
Sbjct: 1604 --------KMEKGGPRSALFKLCKILQWPMPEFEFVEQRFRTPIVMDGATTTNFNSFVST 1620

BLAST of MS002366 vs. ExPASy TrEMBL
Match: A0A6J1C050 (dicer-like protein 4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007071 PE=3 SV=1)

HSP 1 Score: 3233.0 bits (8381), Expect = 0.0e+00
Identity = 1631/1661 (98.19%), Postives = 1636/1661 (98.49%), Query Frame = 0

Query: 1    MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPSTRASEKDPRRKARKYQLEL 60
            MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPS+RASEKDPRRKARKYQLEL
Sbjct: 2    MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPSSRASEKDPRRKARKYQLEL 61

Query: 61   CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE 120
            CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE
Sbjct: 62   CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE 121

Query: 121  DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFD 180
            DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLR LHHCYIKMEFVELLIFD
Sbjct: 122  DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRTLHHCYIKMEFVELLIFD 181

Query: 181  ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLS 240
            ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGK         GACNQQNLS
Sbjct: 182  ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGK---------GACNQQNLS 241

Query: 241  KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKR 300
            KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKR
Sbjct: 242  KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKR 301

Query: 301  QCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSEL 360
            QCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSEL
Sbjct: 302  QCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSEL 361

Query: 361  IEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEIL 420
            IEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEIL
Sbjct: 362  IEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEIL 421

Query: 421  SSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHI 480
            SSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHI
Sbjct: 422  SSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHI 481

Query: 481  LMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540
            LMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV
Sbjct: 482  LMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 541

Query: 541  DSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLL 600
            DSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLL
Sbjct: 542  DSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLL 601

Query: 601  HEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACI 660
            HEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACI
Sbjct: 602  HEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACI 661

Query: 661  KAIEELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSW 720
            KAIEELHKLGALNDFLL MRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSW
Sbjct: 662  KAIEELHKLGALNDFLLPMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSW 721

Query: 721  TGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVP 780
            TGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKA LPQEAERMDIELHLARGRSVMVNLVP
Sbjct: 722  TGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAALPQEAERMDIELHLARGRSVMVNLVP 781

Query: 781  SGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHD 840
            SGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHD
Sbjct: 782  SGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHD 841

Query: 841  NERSVSIDWKVIRRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYK 900
            NERSVSIDWKVIRRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYK
Sbjct: 842  NERSVSIDWKVIRRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYK 901

Query: 901  REFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNL 960
            REFFFVTDIVKGKNGYSRSHYKNSSS SHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNL
Sbjct: 902  REFFFVTDIVKGKNGYSRSHYKNSSSSSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNL 961

Query: 961  LHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIEL 1020
            LHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIEL
Sbjct: 962  LHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIEL 1021

Query: 1021 KCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKS 1080
            KCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKS
Sbjct: 1022 KCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKS 1081

Query: 1081 DEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDI 1140
            DEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDI
Sbjct: 1082 DEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDI 1141

Query: 1141 HSHCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQV 1200
            HSHCNATKNA+ASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQV
Sbjct: 1142 HSHCNATKNAQASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQV 1201

Query: 1201 EFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCY 1260
            EFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCY
Sbjct: 1202 EFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCY 1261

Query: 1261 QRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICD 1320
            QRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICD
Sbjct: 1262 QRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICD 1321

Query: 1321 STSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIM 1380
            STSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIM
Sbjct: 1322 STSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIM 1381

Query: 1381 LSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHATASA 1440
            LSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEA+VKGDNFHATASA
Sbjct: 1382 LSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEADVKGDNFHATASA 1441

Query: 1441 AHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTIDQA 1500
            AHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVT+DQA
Sbjct: 1442 AHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTMDQA 1501

Query: 1501 DNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFDA- 1560
            DNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFD  
Sbjct: 1502 DNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFDGH 1561

Query: 1561 -------VSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEE 1620
                    S GRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEE
Sbjct: 1562 DSPTDVQSSSGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEE 1621

Query: 1621 APNTIFKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYLPETD 1654
            AP+TIFKFFGAPHLRKKAAAEHAAEAAIWYLKK GYLPETD
Sbjct: 1622 APDTIFKFFGAPHLRKKAAAEHAAEAAIWYLKKEGYLPETD 1653

BLAST of MS002366 vs. ExPASy TrEMBL
Match: A0A6J1BZQ8 (dicer-like protein 4 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007071 PE=4 SV=1)

HSP 1 Score: 2755.3 bits (7141), Expect = 0.0e+00
Identity = 1389/1409 (98.58%), Postives = 1394/1409 (98.94%), Query Frame = 0

Query: 253  KVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKRQCIVVLGQMKGE 312
            +VYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKRQCIVVLGQMKGE
Sbjct: 10   EVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKRQCIVVLGQMKGE 69

Query: 313  HEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNHS 372
            HEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNHS
Sbjct: 70   HEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNHS 129

Query: 373  LSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCI 432
            LSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCI
Sbjct: 130  LSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCI 189

Query: 433  IFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLL 492
            IFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLL
Sbjct: 190  IFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLL 249

Query: 493  VATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLID 552
            VATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLID
Sbjct: 250  VATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLID 309

Query: 553  EFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEY 612
            EFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEY
Sbjct: 310  EFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEY 369

Query: 613  FDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGAL 672
            FDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGAL
Sbjct: 370  FDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGAL 429

Query: 673  NDFLLLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSWTGSGYFILNCYQ 732
            NDFLL MRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSWTGSGYFILNCYQ
Sbjct: 430  NDFLLPMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSWTGSGYFILNCYQ 489

Query: 733  IKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEIT 792
            IKFSPHPRDRIYKEFGLFVKA LPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEIT
Sbjct: 490  IKFSPHPRDRIYKEFGLFVKAALPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEIT 549

Query: 793  QAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHDNERSVSIDWKVI 852
            QAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHDNERSVSIDWKVI
Sbjct: 550  QAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHDNERSVSIDWKVI 609

Query: 853  RRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKG 912
            RRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKG
Sbjct: 610  RRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKG 669

Query: 913  KNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEAC 972
            KNGYSRSHYKNSSS SHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEAC
Sbjct: 670  KNGYSRSHYKNSSSSSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEAC 729

Query: 973  YLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGA 1032
            YLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGA
Sbjct: 730  YLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGA 789

Query: 1033 EVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYL 1092
            EVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYL
Sbjct: 790  EVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYL 849

Query: 1093 VKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDIHSHCNATKNAKA 1152
            VKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDIHSHCNATKNA+A
Sbjct: 850  VKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDIHSHCNATKNAQA 909

Query: 1153 SETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALM 1212
            SETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALM
Sbjct: 910  SETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALM 969

Query: 1213 SSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCYQRLEFLGDAVLD 1272
            SSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCYQRLEFLGDAVLD
Sbjct: 970  SSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCYQRLEFLGDAVLD 1029

Query: 1273 YLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYV 1332
            YLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYV
Sbjct: 1030 YLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYV 1089

Query: 1333 NFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSG 1392
            NFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSG
Sbjct: 1090 NFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSG 1149

Query: 1393 FQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHATASAAHRRKRDAKKIA 1452
            FQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEA+VKGDNFHATASAAHRRKRDAKKIA
Sbjct: 1150 FQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEADVKGDNFHATASAAHRRKRDAKKIA 1209

Query: 1453 ANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTIDQADNGFGTIKGLES 1512
            ANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVT+DQADNGFGTIKGLES
Sbjct: 1210 ANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTMDQADNGFGTIKGLES 1269

Query: 1513 SGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFDA--------VSLGR 1572
            SGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFD          S GR
Sbjct: 1270 SGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFDGHDSPTDVQSSSGR 1329

Query: 1573 LCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEAPNTIFKFFGAP 1632
            LCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEAP+TIFKFFGAP
Sbjct: 1330 LCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEAPDTIFKFFGAP 1389

Query: 1633 HLRKKAAAEHAAEAAIWYLKKAGYLPETD 1654
            HLRKKAAAEHAAEAAIWYLKK GYLPETD
Sbjct: 1390 HLRKKAAAEHAAEAAIWYLKKEGYLPETD 1418

BLAST of MS002366 vs. ExPASy TrEMBL
Match: A0A6J1JAJ5 (dicer-like protein 4 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485046 PE=3 SV=1)

HSP 1 Score: 2680.2 bits (6946), Expect = 0.0e+00
Identity = 1376/1676 (82.10%), Postives = 1468/1676 (87.59%), Query Frame = 0

Query: 1    MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPSTRASEKDPRRKARKYQLEL 60
            MPDG+TNS  ADS NFQ T    VE F+GLS VDDQ  G S R S KDPRR ARKYQLEL
Sbjct: 1    MPDGETNSTTADSVNFQPTISASVERFMGLSPVDDQGEGSSIRTSGKDPRRIARKYQLEL 60

Query: 61   CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE 120
            CKKALEENII+YLGTGCGKTHIA+LL+YEM HLIRR QKGICVFLAPTVALVQQQAKVIE
Sbjct: 61   CKKALEENIIVYLGTGCGKTHIAILLIYEMGHLIRRPQKGICVFLAPTVALVQQQAKVIE 120

Query: 121  DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFD 180
            DS DFKV+VYCGGSKCLNSHYDWEREMEE+EVFVMTPEILLRNLHHCYIKMEFVELLIFD
Sbjct: 121  DSIDFKVKVYCGGSKCLNSHYDWEREMEEYEVFVMTPEILLRNLHHCYIKMEFVELLIFD 180

Query: 181  ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLS 240
            ECHHAQVKSDHSYAEIMRVFYK N  KRPRIFGMTASPVVGK         GACNQQNLS
Sbjct: 181  ECHHAQVKSDHSYAEIMRVFYKANEHKRPRIFGMTASPVVGK---------GACNQQNLS 240

Query: 241  KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKR 300
            KSINSLEKLLDAKVYSVEDREEL+AFVSSP  KIYYYGPVA G S SFMTYC  LED+K 
Sbjct: 241  KSINSLEKLLDAKVYSVEDREELEAFVSSPRFKIYYYGPVATGSSSSFMTYCCPLEDVKC 300

Query: 301  QCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSEL 360
            QCIV LGQMKGEHE L ATKKLLCRMH NIIFCLENLGVWGALQASKILLS DNSERSEL
Sbjct: 301  QCIVALGQMKGEHEGLLATKKLLCRMHKNIIFCLENLGVWGALQASKILLSEDNSERSEL 360

Query: 361  IEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEIL 420
            IEAAEGNP   S+SDRYLNQAAEI A GCKI  G+SD LN DIL+ P FSKKLL+LI IL
Sbjct: 361  IEAAEGNPKYDSISDRYLNQAAEIFASGCKIDAGVSDMLNVDILKEPCFSKKLLRLIGIL 420

Query: 421  SSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHI 480
            SSFRHR NMKCIIFVNRIVIARSLSYILQNL+FLA+WKCD+LVGVHSKLRSMSRKTMNHI
Sbjct: 421  SSFRHRPNMKCIIFVNRIVIARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNHI 480

Query: 481  LMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540
            L KFRSGELNLL+ATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV
Sbjct: 481  LTKFRSGELNLLIATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540

Query: 541  DSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLL 600
            DSGNEK+LGLIDEF KDE RM+REISSRSS E F+ HEESIYRV STGAS+TSGRSISLL
Sbjct: 541  DSGNEKDLGLIDEFHKDEKRMNREISSRSSIETFDGHEESIYRVVSTGASVTSGRSISLL 600

Query: 601  HEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACI 660
            HE+CSKLPHD YFDPKPKFFY DDL GT+CHAILPSNAPI QI+SR QPS   AKKDAC+
Sbjct: 601  HEYCSKLPHDTYFDPKPKFFYCDDLGGTICHAILPSNAPIPQIVSRPQPSGVAAKKDACL 660

Query: 661  KAIEELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSW 720
            KA+EELHKLGAL+D+LL MRD+ANE ELELD SDSD SEDETSR ELHEM+ PAA K+SW
Sbjct: 661  KAVEELHKLGALSDYLLPMRDNANEGELELD-SDSDSSEDETSRLELHEMISPAAFKESW 720

Query: 721  TGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVP 780
            TG GY IL CY IKF+P+P DRIYKEFGLFVKAPLPQEA RMD+ELHLA GRSV VNL+P
Sbjct: 721  TGFGYVILYCYHIKFTPNPEDRIYKEFGLFVKAPLPQEAVRMDLELHLAHGRSVKVNLMP 780

Query: 781  SGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHD 840
            S VVELLE+EITQAE FQEMFLKVILDRSEFV ++IPLRNNVS+SISSTSYLLLP+I HD
Sbjct: 781  SRVVELLENEITQAECFQEMFLKVILDRSEFVHDYIPLRNNVSRSISSTSYLLLPVIVHD 840

Query: 841  NERSVSIDWKVIRRCLFSPIFQIPG-SIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPY 900
            NE SV IDWKVIRRCL S IFQ    SI+KETA S  HL LYDGRRR+SDI+NSLVYVPY
Sbjct: 841  NEGSVFIDWKVIRRCLSSQIFQNHACSIIKETASSDTHLMLYDGRRRSSDIENSLVYVPY 900

Query: 901  KREFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHN 960
            KREFFFVT+IV+ KNGYS+  YK+S SLSHFEHL  RFGIHLKYPEQPLL AKPLF LHN
Sbjct: 901  KREFFFVTNIVRRKNGYSQ--YKDSGSLSHFEHLKTRFGIHLKYPEQPLLCAKPLFSLHN 960

Query: 961  LLHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIE 1020
            LLH+R+Q +SEA YL+EYFID+ PELCQLKI GFSKDIGSSISLLPSIMHRLENLLVAIE
Sbjct: 961  LLHNRKQKDSEAPYLEEYFIDVAPELCQLKITGFSKDIGSSISLLPSIMHRLENLLVAIE 1020

Query: 1021 LKCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDK 1080
            LKCMLAAAFP GAEVTANRILEALTTEKCQER SLERLEILGD FLKFAVARYLFL HDK
Sbjct: 1021 LKCMLAAAFPAGAEVTANRILEALTTEKCQERFSLERLEILGDSFLKFAVARYLFLAHDK 1080

Query: 1081 SDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETRED 1140
             DEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFY LGRPCPRICNEETR D
Sbjct: 1081 FDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYLLGRPCPRICNEETRND 1140

Query: 1141 IHSHCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQ 1200
            IHSH NAT NAK SETKCS+GHHWL KKTISD+VEAL+GAFLVDSGFKAAI+FLKWIGI+
Sbjct: 1141 IHSHDNATNNAKFSETKCSKGHHWLQKKTISDMVEALIGAFLVDSGFKAAIAFLKWIGIK 1200

Query: 1201 VEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGC 1260
            VEFEAS VTNAL++S AY++ AD INIS LQ+SLGH+FLHKGLLLQALVHP+YHKHGGGC
Sbjct: 1201 VEFEASQVTNALVASKAYVLFADTINISALQSSLGHKFLHKGLLLQALVHPTYHKHGGGC 1260

Query: 1261 YQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLIC 1320
            YQRLEFLGDAVLDYLI SYLYS YPKLKPGLLTDLRSVFVRNEAFANVAVDR FYK+L+C
Sbjct: 1261 YQRLEFLGDAVLDYLIMSYLYSVYPKLKPGLLTDLRSVFVRNEAFANVAVDRFFYKFLLC 1320

Query: 1321 DSTSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKI 1380
            DS SL+SDIKSYV+FIKAP S   SL  P CPK LGDLVES VGAVLVDTGFDMNYVWKI
Sbjct: 1321 DSASLESDIKSYVDFIKAPPSERDSLEQPRCPKPLGDLVESSVGAVLVDTGFDMNYVWKI 1380

Query: 1381 MLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHATAS 1440
            MLSFLDPI+S SGFQLSPIRDIIE CQ+CG  LQFN SK  KYYSVEAEVKGD+FHATAS
Sbjct: 1381 MLSFLDPIMSSSGFQLSPIRDIIEVCQNCGWKLQFNPSKMEKYYSVEAEVKGDDFHATAS 1440

Query: 1441 AAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTIDQ 1500
            A +RRK+DA+KIAAN IL KLKAQG IPEVNSLE+ILKSS KM+PKLIGYDETPSVT+D+
Sbjct: 1441 AVNRRKKDAEKIAANAILRKLKAQGFIPEVNSLEKILKSSEKMKPKLIGYDETPSVTMDR 1500

Query: 1501 ADNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFD- 1560
             D+      GLE SGADLDP VH EVGN+ P RITR+S+ L SSSGAA EQL PS+  + 
Sbjct: 1501 IDD------GLEFSGADLDPLVHREVGNSRPFRITRMSETLASSSGAAGEQLKPSIPLEG 1560

Query: 1561 ---------------------AVSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPS 1620
                                 + S GR  K TARSRLYE+CA NHW+ PSF+CMNEEGPS
Sbjct: 1561 HDSSTDLLSSSSSSSSSSSSSSSSSGRSGKTTARSRLYEVCAINHWNHPSFNCMNEEGPS 1620

Query: 1621 HLKMFTYKVVLEIEEAPNTIFKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYLPETD 1654
            HLKMFTYKVVLEIEEAP+TIF+FFGAPHL KKAAAEHAAEAA+WYLKK  YL +TD
Sbjct: 1621 HLKMFTYKVVLEIEEAPDTIFEFFGAPHLTKKAAAEHAAEAALWYLKKERYLGDTD 1658

BLAST of MS002366 vs. ExPASy TrEMBL
Match: A0A6J1E1P4 (dicer-like protein 4 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429985 PE=3 SV=1)

HSP 1 Score: 2676.7 bits (6937), Expect = 0.0e+00
Identity = 1377/1672 (82.36%), Postives = 1469/1672 (87.86%), Query Frame = 0

Query: 1    MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPSTRASEKDPRRKARKYQLEL 60
            MPDG+ NS  ADS NFQ TS   VE F+GLS VDDQ  G S R S KDPRR ARKYQLEL
Sbjct: 1    MPDGEINSTTADSVNFQPTSSASVERFMGLSPVDDQGAGSSIRTSGKDPRRIARKYQLEL 60

Query: 61   CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE 120
            C+KALEENII+YLGTGCGKTHIA+LL+YEM HLIRR QKGICVFLAPTVALVQQQAKVIE
Sbjct: 61   CEKALEENIIVYLGTGCGKTHIAILLIYEMGHLIRRPQKGICVFLAPTVALVQQQAKVIE 120

Query: 121  DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFD 180
            DS DFKV+VYCGGSKCLNSHYDWEREMEE+EVFVMTPEILLRNLHHCYI+MEFVELLIFD
Sbjct: 121  DSIDFKVKVYCGGSKCLNSHYDWEREMEEYEVFVMTPEILLRNLHHCYIRMEFVELLIFD 180

Query: 181  ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLS 240
            ECHHAQVKSDHSYAEIMRVFYK N  KRPRIFGMTASPVVGK         GACNQQNLS
Sbjct: 181  ECHHAQVKSDHSYAEIMRVFYKANEHKRPRIFGMTASPVVGK---------GACNQQNLS 240

Query: 241  KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKR 300
            KSINSLEKLLDAKVYSVEDREEL+AFVSSP  KIYYYGPVA G S SFMTYC  LED+K 
Sbjct: 241  KSINSLEKLLDAKVYSVEDREELEAFVSSPRFKIYYYGPVATGSSSSFMTYCCPLEDVKC 300

Query: 301  QCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSEL 360
            QCIV LGQMKGEHE L ATKKLLCRMH NIIFCLENLGVWGALQASKILLS DNSERSEL
Sbjct: 301  QCIVALGQMKGEHEGLLATKKLLCRMHKNIIFCLENLGVWGALQASKILLSEDNSERSEL 360

Query: 361  IEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEIL 420
            IEAA  NP + S+SDRYLNQAAEI A GCKI  G+SD LN DIL+ P FSKKLL+LI IL
Sbjct: 361  IEAAAANPKHDSISDRYLNQAAEIFASGCKIDAGVSDMLNVDILKEPCFSKKLLRLIGIL 420

Query: 421  SSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHI 480
            SSFRHR NMKCIIFVNRIVIARSLSYILQNL+FLA+WKCD+LVGVHSKLRSMSRKTMNHI
Sbjct: 421  SSFRHRPNMKCIIFVNRIVIARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNHI 480

Query: 481  LMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540
            L KFRSGELNLL+ATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV
Sbjct: 481  LTKFRSGELNLLIATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540

Query: 541  DSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLL 600
            DSGNEKELGLIDEF KDE RM+REISSRSS E F+ HEESIYRV STGAS+TSGRSISLL
Sbjct: 541  DSGNEKELGLIDEFHKDEKRMNREISSRSSVETFDGHEESIYRVISTGASVTSGRSISLL 600

Query: 601  HEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACI 660
            HE+CSKLPHD YFDPKPKFFY DDL GT+CHAILPSNAPI QI+SR QPS   AKKDAC+
Sbjct: 601  HEYCSKLPHDTYFDPKPKFFYCDDLGGTICHAILPSNAPIPQIVSRPQPSGVAAKKDACL 660

Query: 661  KAIEELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSW 720
            KA+EELHKLGAL+D+LL MRD+ANE ELELD SDSD SEDETSR ELHEM+ PAA K+SW
Sbjct: 661  KAVEELHKLGALSDYLLPMRDNANEGELELD-SDSDSSEDETSRRELHEMISPAAFKESW 720

Query: 721  TGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVP 780
            TG GY IL CY IKF+P+P DRIYKEFGLFVKAPLPQEA RMD+ELHLA GRSV VNL+P
Sbjct: 721  TGFGYVILYCYHIKFTPNPEDRIYKEFGLFVKAPLPQEAVRMDLELHLAHGRSVKVNLMP 780

Query: 781  SGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHD 840
            S VVEL+E+EITQAE FQEMFLKVILDRSEFV ++IPLRNNVS+SISSTSYLLLP+I HD
Sbjct: 781  SRVVELMENEITQAECFQEMFLKVILDRSEFVHDYIPLRNNVSRSISSTSYLLLPVIVHD 840

Query: 841  NERSVSIDWKVIRRCLFSPIFQIPG-SIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPY 900
             E SV IDWKVIRRCL S IFQ    SIVKETA S  HL LYDGRRR+SDI+NSLVYVPY
Sbjct: 841  IEGSVFIDWKVIRRCLSSQIFQNHACSIVKETASSDTHLILYDGRRRSSDIENSLVYVPY 900

Query: 901  KREFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHN 960
            KREFFFVT+IV+ KNGYS+  YK+S SLSHFEHL  RFGI LKYPEQPLL AKPLF LHN
Sbjct: 901  KREFFFVTNIVRRKNGYSQ--YKDSGSLSHFEHLKTRFGIQLKYPEQPLLCAKPLFSLHN 960

Query: 961  LLHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIE 1020
            LLHDR+Q +SEA YL+EYFID+ PELCQLKI GFSKDIGSSISLLPSIMHRLENLLVAIE
Sbjct: 961  LLHDRKQKDSEAPYLEEYFIDIAPELCQLKITGFSKDIGSSISLLPSIMHRLENLLVAIE 1020

Query: 1021 LKCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDK 1080
            LKCMLAA FP GAEVTANRILEALTTEKCQERISLERLEILGD FLKFAVARYLFL HDK
Sbjct: 1021 LKCMLAAGFPAGAEVTANRILEALTTEKCQERISLERLEILGDSFLKFAVARYLFLAHDK 1080

Query: 1081 SDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETRED 1140
             DEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFY LGRPCPRICNEETR D
Sbjct: 1081 FDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYLLGRPCPRICNEETRND 1140

Query: 1141 IHSHCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQ 1200
            IHSH NAT NAKASETKCS+GHHWL KKTISDVVEALVGAFLVDSGFKAAI+FLKWIGI+
Sbjct: 1141 IHSHDNATNNAKASETKCSKGHHWLQKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIK 1200

Query: 1201 VEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGC 1260
            VEFEAS VTNAL++S AY++LAD INIS LQ+SLG++FLHKGLLLQALVHP+YHKHGGGC
Sbjct: 1201 VEFEASQVTNALVASKAYVLLADTINISALQSSLGYKFLHKGLLLQALVHPTYHKHGGGC 1260

Query: 1261 YQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLIC 1320
            YQRLEFLGDAVLDYLI SYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDR FYK+L+C
Sbjct: 1261 YQRLEFLGDAVLDYLIMSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRFFYKFLLC 1320

Query: 1321 DSTSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKI 1380
            DS SLQSDIKSYV+FIK+  S   SL  P CPK LGDLVES VGAVLVDTGFDMNYVWKI
Sbjct: 1321 DSASLQSDIKSYVDFIKSLPSERDSLEQPRCPKPLGDLVESSVGAVLVDTGFDMNYVWKI 1380

Query: 1381 MLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHATAS 1440
            MLSFLDPI+S SGFQLSPIRDIIE CQ+CG  LQFN SK  KYYSVEAEVKGD+FHATAS
Sbjct: 1381 MLSFLDPIMSSSGFQLSPIRDIIEVCQNCGWKLQFNPSKMEKYYSVEAEVKGDDFHATAS 1440

Query: 1441 AAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTIDQ 1500
            A +RRK+DA+KIAAN ILTKLKAQG IPEVNSLE+ILKSS KM+PKLIGYDETPSVT+DQ
Sbjct: 1441 AVNRRKKDAEKIAANSILTKLKAQGFIPEVNSLEKILKSSEKMKPKLIGYDETPSVTMDQ 1500

Query: 1501 ADNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFD- 1560
             D+      GLE SGADLDP VH EVGN+HP RITR+S+ L SSSGAA EQL PS+  + 
Sbjct: 1501 IDD------GLEFSGADLDPRVHHEVGNSHPFRITRMSETLASSSGAAGEQLKPSIPLEG 1560

Query: 1561 -----------------AVSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKM 1620
                             + S GR  K TARSRL+E+CA NHW+ PSF+CMNEEGPSHLKM
Sbjct: 1561 HDSSTDLLSSSSSSSSSSSSSGRSGKTTARSRLHEVCAINHWNHPSFNCMNEEGPSHLKM 1620

Query: 1621 FTYKVVLEIEEAPNTIFKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYLPETD 1654
            FTYKVVLEIEEAP+TIF+FFGAPHL KKAAAEHAAEAA+WYLKK  YL +TD
Sbjct: 1621 FTYKVVLEIEEAPDTIFEFFGAPHLTKKAAAEHAAEAALWYLKKERYLGDTD 1654

BLAST of MS002366 vs. ExPASy TrEMBL
Match: A0A6J1JJM5 (dicer-like protein 4 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485046 PE=3 SV=1)

HSP 1 Score: 2673.7 bits (6929), Expect = 0.0e+00
Identity = 1375/1676 (82.04%), Postives = 1467/1676 (87.53%), Query Frame = 0

Query: 1    MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPSTRASEKDPRRKARKYQLEL 60
            MPDG+TNS  ADS NFQ T    VE F+GLS VDDQ  G S R S KDPRR ARKYQLEL
Sbjct: 1    MPDGETNSTTADSVNFQPTISASVERFMGLSPVDDQGEGSSIRTSGKDPRRIARKYQLEL 60

Query: 61   CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE 120
            CKKALEENII+YLGTGCGKTHIA+LL+YEM HLIRR QKGICVFLAPTVALVQQQAKVIE
Sbjct: 61   CKKALEENIIVYLGTGCGKTHIAILLIYEMGHLIRRPQKGICVFLAPTVALVQQQAKVIE 120

Query: 121  DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFD 180
            DS DFKV+VYCGGSKCLNSHYDWEREMEE+EVFVMTPEILLRNLHHCYIKMEFVELLIFD
Sbjct: 121  DSIDFKVKVYCGGSKCLNSHYDWEREMEEYEVFVMTPEILLRNLHHCYIKMEFVELLIFD 180

Query: 181  ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLS 240
            ECHHAQVKSDHSYAEIMRVFYK N  KRPRIFGMTASPVVGK         GACNQQNLS
Sbjct: 181  ECHHAQVKSDHSYAEIMRVFYKANEHKRPRIFGMTASPVVGK---------GACNQQNLS 240

Query: 241  KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKR 300
            KSINSLEKLLDAKVYSVEDREEL+AFVSSP  KIYYYGPVA G S SFMTYC  LED+K 
Sbjct: 241  KSINSLEKLLDAKVYSVEDREELEAFVSSPRFKIYYYGPVATGSSSSFMTYCCPLEDVKC 300

Query: 301  QCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSEL 360
            QCIV LGQMKGEHE L ATKKLLCRMH NIIFCLENLGVWGALQASKILLS DNSERSEL
Sbjct: 301  QCIVALGQMKGEHEGLLATKKLLCRMHKNIIFCLENLGVWGALQASKILLSEDNSERSEL 360

Query: 361  IEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEIL 420
            IEAAEGNP   S+SDRYLNQAAEI A GCKI  G+SD LN DIL+ P FSKKLL+LI IL
Sbjct: 361  IEAAEGNPKYDSISDRYLNQAAEIFASGCKIDAGVSDMLNVDILKEPCFSKKLLRLIGIL 420

Query: 421  SSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHI 480
            SSFRHR NMKCIIFVNRIVIARSLSYILQNL+FLA+WKCD+LVGVHSKLRSMSRKTMNHI
Sbjct: 421  SSFRHRPNMKCIIFVNRIVIARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNHI 480

Query: 481  LMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540
            L KFRSGELNLL+ATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV
Sbjct: 481  LTKFRSGELNLLIATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540

Query: 541  DSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLL 600
            DSGNEK+LGLIDEF KDE RM+REISSRSS E F+ HEESIYRV STGAS+TSGRSISLL
Sbjct: 541  DSGNEKDLGLIDEFHKDEKRMNREISSRSSIETFDGHEESIYRVVSTGASVTSGRSISLL 600

Query: 601  HEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACI 660
            HE+CSKLPHD YFDPKPKFFY DDL GT+CHAILPSNAPI QI+SR QPS   AKKDAC+
Sbjct: 601  HEYCSKLPHDTYFDPKPKFFYCDDLGGTICHAILPSNAPIPQIVSRPQPSGVAAKKDACL 660

Query: 661  KAIEELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSW 720
            KA+EELHKLGAL+D+LL MRD+ANE ELELD SDSD SEDETSR ELHEM+ PAA K+SW
Sbjct: 661  KAVEELHKLGALSDYLLPMRDNANEGELELD-SDSDSSEDETSRLELHEMISPAAFKESW 720

Query: 721  TGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVP 780
            TG GY IL CY IKF+P+P DRIYKEFGLFVKAPLPQEA RMD+ELHLA GRSV VNL+P
Sbjct: 721  TGFGYVILYCYHIKFTPNPEDRIYKEFGLFVKAPLPQEAVRMDLELHLAHGRSVKVNLMP 780

Query: 781  SGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHD 840
            S VVELLE+EITQAE FQEMFLKVILDRSEFV ++IPLRNNVS+SISSTSYLLLP+I HD
Sbjct: 781  SRVVELLENEITQAECFQEMFLKVILDRSEFVHDYIPLRNNVSRSISSTSYLLLPVIVHD 840

Query: 841  NERSVSIDWKVIRRCLFSPIFQIPG-SIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPY 900
            NE SV IDWKVIRRCL S IFQ    SI+KETA S  HL LYDGRRR+SDI+NSLVYVPY
Sbjct: 841  NEGSVFIDWKVIRRCLSSQIFQNHACSIIKETASSDTHLMLYDGRRRSSDIENSLVYVPY 900

Query: 901  KREFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHN 960
            KREFFFVT+IV+ KNGYS+  YK+S SLSHFEHL  RFGIHLKYPEQPLL AKPLF LHN
Sbjct: 901  KREFFFVTNIVRRKNGYSQ--YKDSGSLSHFEHLKTRFGIHLKYPEQPLLCAKPLFSLHN 960

Query: 961  LLHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIE 1020
            LLH+R+Q +S A YL+EYFID+ PELCQLKI GFSKDIGSSISLLPSIMHRLENLLVAIE
Sbjct: 961  LLHNRKQKDS-APYLEEYFIDVAPELCQLKITGFSKDIGSSISLLPSIMHRLENLLVAIE 1020

Query: 1021 LKCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDK 1080
            LKCMLAAAFP GAEVTANRILEALTTEKCQER SLERLEILGD FLKFAVARYLFL HDK
Sbjct: 1021 LKCMLAAAFPAGAEVTANRILEALTTEKCQERFSLERLEILGDSFLKFAVARYLFLAHDK 1080

Query: 1081 SDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETRED 1140
             DEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFY LGRPCPRICNEETR D
Sbjct: 1081 FDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYLLGRPCPRICNEETRND 1140

Query: 1141 IHSHCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQ 1200
            IHSH NAT NAK SETKCS+GHHWL KKTISD+VEAL+GAFLVDSGFKAAI+FLKWIGI+
Sbjct: 1141 IHSHDNATNNAKFSETKCSKGHHWLQKKTISDMVEALIGAFLVDSGFKAAIAFLKWIGIK 1200

Query: 1201 VEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGC 1260
            VEFEAS VTNAL++S AY++ AD INIS LQ+SLGH+FLHKGLLLQALVHP+YHKHGGGC
Sbjct: 1201 VEFEASQVTNALVASKAYVLFADTINISALQSSLGHKFLHKGLLLQALVHPTYHKHGGGC 1260

Query: 1261 YQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLIC 1320
            YQRLEFLGDAVLDYLI SYLYS YPKLKPGLLTDLRSVFVRNEAFANVAVDR FYK+L+C
Sbjct: 1261 YQRLEFLGDAVLDYLIMSYLYSVYPKLKPGLLTDLRSVFVRNEAFANVAVDRFFYKFLLC 1320

Query: 1321 DSTSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKI 1380
            DS SL+SDIKSYV+FIKAP S   SL  P CPK LGDLVES VGAVLVDTGFDMNYVWKI
Sbjct: 1321 DSASLESDIKSYVDFIKAPPSERDSLEQPRCPKPLGDLVESSVGAVLVDTGFDMNYVWKI 1380

Query: 1381 MLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHATAS 1440
            MLSFLDPI+S SGFQLSPIRDIIE CQ+CG  LQFN SK  KYYSVEAEVKGD+FHATAS
Sbjct: 1381 MLSFLDPIMSSSGFQLSPIRDIIEVCQNCGWKLQFNPSKMEKYYSVEAEVKGDDFHATAS 1440

Query: 1441 AAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTIDQ 1500
            A +RRK+DA+KIAAN IL KLKAQG IPEVNSLE+ILKSS KM+PKLIGYDETPSVT+D+
Sbjct: 1441 AVNRRKKDAEKIAANAILRKLKAQGFIPEVNSLEKILKSSEKMKPKLIGYDETPSVTMDR 1500

Query: 1501 ADNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFD- 1560
             D+      GLE SGADLDP VH EVGN+ P RITR+S+ L SSSGAA EQL PS+  + 
Sbjct: 1501 IDD------GLEFSGADLDPLVHREVGNSRPFRITRMSETLASSSGAAGEQLKPSIPLEG 1560

Query: 1561 ---------------------AVSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPS 1620
                                 + S GR  K TARSRLYE+CA NHW+ PSF+CMNEEGPS
Sbjct: 1561 HDSSTDLLSSSSSSSSSSSSSSSSSGRSGKTTARSRLYEVCAINHWNHPSFNCMNEEGPS 1620

Query: 1621 HLKMFTYKVVLEIEEAPNTIFKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYLPETD 1654
            HLKMFTYKVVLEIEEAP+TIF+FFGAPHL KKAAAEHAAEAA+WYLKK  YL +TD
Sbjct: 1621 HLKMFTYKVVLEIEEAPDTIFEFFGAPHLTKKAAAEHAAEAALWYLKKERYLGDTD 1657

BLAST of MS002366 vs. TAIR 10
Match: AT5G20320.1 (dicer-like 4 )

HSP 1 Score: 1682.9 bits (4357), Expect = 0.0e+00
Identity = 892/1652 (54.00%), Postives = 1145/1652 (69.31%), Query Frame = 0

Query: 13   SDNFQHTSLGPVESFVG-----LSLVDDQEPGPSTRASEKDPRRKARKYQLELCKKALEE 72
            S +   TS   V SF        S     +P P     EKDPR+ AR+YQ+ELCKKA EE
Sbjct: 82   SSDLHLTSSSSVSSFSSSSSSLFSAAGTDDPSPK---MEKDPRKIARRYQVELCKKATEE 141

Query: 73   NIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIEDSTDFKV 132
            N+I+YLGTGCGKTHIAV+L+YE+ HL+   +K +C+FLAPTVALV+QQAKVI DS +FKV
Sbjct: 142  NVIVYLGTGCGKTHIAVMLIYELGHLVLSPKKSVCIFLAPTVALVEQQAKVIADSVNFKV 201

Query: 133  RVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFDECHHAQV 192
             ++CGG + + SH +WERE+  +EV VMTP+ILL NL HC+IKME + LLIFDECHHAQ 
Sbjct: 202  AIHCGGKRIVKSHSEWEREIAANEVLVMTPQILLHNLQHCFIKMECISLLIFDECHHAQQ 261

Query: 193  KSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLSKSINSLE 252
            +S+H YAEIM+VFYK+   +RPRIFGMTASPVVGK         G+   +NLSKSINSLE
Sbjct: 262  QSNHPYAEIMKVFYKSESLQRPRIFGMTASPVVGK---------GSFQSENLSKSINSLE 321

Query: 253  KLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKRQCIVVLG 312
             LL+AKVYSVE   +LD FVSSPLVK+YYY    +  S S + Y ++LED+K++C+  L 
Sbjct: 322  NLLNAKVYSVESNVQLDGFVSSPLVKVYYYRSALSDASQSTIRYENMLEDIKQRCLASLK 381

Query: 313  QMKGEH--EDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAE 372
             +   H  + L + K+LL R HDN+I+ L NLG+WGA+QA+KI L+ D++ + E +    
Sbjct: 382  LLIDTHQTQTLLSMKRLLKRSHDNLIYTLLNLGLWGAIQAAKIQLNSDHNVQDEPV---- 441

Query: 373  GNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRH 432
            G      + D YL+ AAE L+ G       SD L+   L+ P FS+KL+QLI+ILS FR 
Sbjct: 442  GKNPKSKICDTYLSMAAEALSSGVAKDENASDLLSLAALKEPLFSRKLVQLIKILSVFRL 501

Query: 433  RLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFR 492
              +MKCIIFVNRIV AR+LS IL NLE L  WK D+LVG+ S L+SMSR++M  IL +F+
Sbjct: 502  EPHMKCIIFVNRIVTARTLSCILNNLELLRSWKSDFLVGLSSGLKSMSRRSMETILKRFQ 561

Query: 493  SGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNE 552
            S ELNLLVATKVGEEGLDIQTCCLVIR+DLPETV+SFIQSRGRARMPQSEYAFLVDSGNE
Sbjct: 562  SKELNLLVATKVGEEGLDIQTCCLVIRYDLPETVTSFIQSRGRARMPQSEYAFLVDSGNE 621

Query: 553  KELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCS 612
            KE+ LI+ F+ +E+RM+ EI+ RSS E     +E +Y+V  TGA I+ G SISLL+++CS
Sbjct: 622  KEMDLIENFKVNEDRMNLEITYRSSEETCPRLDEELYKVHETGACISGGSSISLLYKYCS 681

Query: 613  KLPHDEYFDPKPKFFY--YDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAI 672
            +LPHDE+F PKP+F +   D+  GT+C   LP+NAPI +I S   PS E AKKDAC+KA+
Sbjct: 682  RLPHDEFFQPKPEFQFKPVDEFGGTICRITLPANAPISEIESSLLPSTEAAKKDACLKAV 741

Query: 673  EELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDE-TSRGELHEMLVPAALKDSWTG 732
             ELH LG LNDFLL    D  E+EL  D  D D  + E  SRG+L+EM VP   K  W  
Sbjct: 742  HELHNLGVLNDFLLPDSKDEIEDELSDDEFDFDNIKGEGCSRGDLYEMRVPVLFKQKWDP 801

Query: 733  SGYFI-LNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVPS 792
            S   + L+ Y I F PHP DRIYK+FG F+K+PLP EAE MDI+LHLA  RSV V + PS
Sbjct: 802  STSCVNLHSYYIMFVPHPADRIYKKFGFFMKSPLPVEAETMDIDLHLAHQRSVSVKIFPS 861

Query: 793  GVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLR-NNVSKSISSTSYLLLPLIFHD 852
            GV E   DEI  AE FQE+ LKV+ +R E + +F+PL   + S++  ST YLLLPL  HD
Sbjct: 862  GVTEFDNDEIRLAELFQEIALKVLFERGELIPDFVPLELQDSSRTSKSTFYLLLPLCLHD 921

Query: 853  NERSVSIDWKVIRRCLFSPIFQIPGSIVKET-ACSHAHLTLYDGRRRTSDIKNSLVYVPY 912
             E  +S+DW  IR CL SPIF+ P  +V++    S +HL L +G     D+KNSLV+  Y
Sbjct: 922  GESVISVDWVTIRNCLSSPIFKTPSVLVEDIFPPSGSHLKLANGCWNIDDVKNSLVFTTY 981

Query: 913  KREFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHN 972
             ++F+FV DI  G+NG+  S  K SS+ SH E + + +G+ LK+P QPLL  KPL H+ N
Sbjct: 982  SKQFYFVADICHGRNGF--SPVKESSTKSHVESIYKLYGVELKHPAQPLLRVKPLCHVRN 1041

Query: 973  LLHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIE 1032
            LLH+R Q N E   L EYFI++PPEL  LKI G SKDIGSS+SLLPSIMHR+ENLLVAIE
Sbjct: 1042 LLHNRMQTNLEPQELDEYFIEIPPELSHLKIKGLSKDIGSSLSLLPSIMHRMENLLVAIE 1101

Query: 1033 LKCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDK 1092
            LK +L+A+ P+ AEV+ +R+LEALTTEKC ER+SLERLE+LGD FLKFAV+R+LFL HD 
Sbjct: 1102 LKHVLSASIPEIAEVSGHRVLEALTTEKCHERLSLERLEVLGDAFLKFAVSRHLFLHHDS 1161

Query: 1093 SDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETRED 1152
             DEGELTRRRS +V N NL +LA +KNLQVYIRDQ  +P+QF+  G PC   C+E   ++
Sbjct: 1162 LDEGELTRRRSNVVNNSNLCRLAIKKNLQVYIRDQALDPTQFFAFGHPCRVTCDEVASKE 1221

Query: 1153 IHS--HCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIG 1212
            +HS         +   E +CS+GHHWL+KKTI+DVVEALVGAFLVDSGFK A+ FLKWIG
Sbjct: 1222 VHSLNRDLGILESNTGEIRCSKGHHWLYKKTIADVVEALVGAFLVDSGFKGAVKFLKWIG 1281

Query: 1213 IQVEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGG 1272
            + V+FE+  V +A ++S  Y+ L  R N+  L+N L ++FLHKGLL+QA +HPSY++HGG
Sbjct: 1282 VNVDFESLQVQDACIASRRYLPLTTRNNLETLENQLDYKFLHKGLLVQAFIHPSYNRHGG 1341

Query: 1273 GCYQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYL 1332
            GCYQRLEFLGDAVLDYL+TSY ++ +PKLKPG LTDLRS+ V NEA ANVAV  S  ++L
Sbjct: 1342 GCYQRLEFLGDAVLDYLMTSYFFTVFPKLKPGQLTDLRSLSVNNEALANVAVSFSLKRFL 1401

Query: 1333 ICDSTSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVW 1392
             C+S  L   I+ Y NF+ +    SG    P CPK LGDLVESC+GA+ +D GF++N+VW
Sbjct: 1402 FCESIYLHEVIEDYTNFLASSPLASGQSEGPRCPKVLGDLVESCLGALFLDCGFNLNHVW 1461

Query: 1393 KIMLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHAT 1452
             +MLSFLDP+ + S  Q+SPI+++IE CQ    + + + +K    ++VE +V  +    T
Sbjct: 1462 TMMLSFLDPVKNLSNLQISPIKELIELCQSYKWDREISATKKDGAFTVELKVTKNGCCLT 1521

Query: 1453 ASAAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTI 1512
             SA  R KR+  K AA L++T LKA   I   + LE++LK+  + E KLIGY+E P   +
Sbjct: 1522 VSATGRNKREGTKKAAQLMITNLKAHENITTSHPLEDVLKNGIRNEAKLIGYNEDPIDVV 1581

Query: 1513 DQADNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAF 1572
            D        +  LE+ G           GN+  +    I + L  +    +++L P  A 
Sbjct: 1582 DLVGLDVENLNILETFG-----------GNSERSSSYVIRRGLPQAPSKTEDRL-PQKAI 1641

Query: 1573 DAVSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEAPNTI 1632
              +  G     TA+S L+E C AN W PP F+C  EEGP HLK F YKV+LE+E+APN  
Sbjct: 1642 --IKAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMT 1701

Query: 1633 FKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYL 1650
             + +G     KK AAEHAA+AAIW LK +G+L
Sbjct: 1702 LECYGEARATKKGAAEHAAQAAIWCLKHSGFL 1701

BLAST of MS002366 vs. TAIR 10
Match: AT5G20320.2 (dicer-like 4 )

HSP 1 Score: 1654.4 bits (4283), Expect = 0.0e+00
Identity = 882/1652 (53.39%), Postives = 1134/1652 (68.64%), Query Frame = 0

Query: 13   SDNFQHTSLGPVESFVG-----LSLVDDQEPGPSTRASEKDPRRKARKYQLELCKKALEE 72
            S +   TS   V SF        S     +P P     EKDPR+ AR+YQ+ELCKKA EE
Sbjct: 82   SSDLHLTSSSSVSSFSSSSSSLFSAAGTDDPSPK---MEKDPRKIARRYQVELCKKATEE 141

Query: 73   NIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIEDSTDFKV 132
            N+I+YLGTGCGKTHIAV+L+YE+ HL+   +K +C+FLAPTVALV+QQAKVI DS +FKV
Sbjct: 142  NVIVYLGTGCGKTHIAVMLIYELGHLVLSPKKSVCIFLAPTVALVEQQAKVIADSVNFKV 201

Query: 133  RVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFDECHHAQV 192
             ++CGG + + SH +WERE+  +E              HC+IKME + LLIFDECHHAQ 
Sbjct: 202  AIHCGGKRIVKSHSEWEREIAANE--------------HCFIKMECISLLIFDECHHAQQ 261

Query: 193  KSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLSKSINSLE 252
            +S+H YAEIM+VFYK+   +RPRIFGMTASPVVGK         G+   +NLSKSINSLE
Sbjct: 262  QSNHPYAEIMKVFYKSESLQRPRIFGMTASPVVGK---------GSFQSENLSKSINSLE 321

Query: 253  KLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKRQCIVVLG 312
             LL+AKVYSVE   +LD FVSSPLVK+YYY    +  S S + Y ++LED+K++C+  L 
Sbjct: 322  NLLNAKVYSVESNVQLDGFVSSPLVKVYYYRSALSDASQSTIRYENMLEDIKQRCLASLK 381

Query: 313  QMKGEH--EDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAE 372
             +   H  + L + K+LL R HDN+I+ L NLG+WGA+QA+KI L+ D++ + E +    
Sbjct: 382  LLIDTHQTQTLLSMKRLLKRSHDNLIYTLLNLGLWGAIQAAKIQLNSDHNVQDEPV---- 441

Query: 373  GNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRH 432
            G      + D YL+ AAE L+ G       SD L+   L+ P FS+KL+QLI+ILS FR 
Sbjct: 442  GKNPKSKICDTYLSMAAEALSSGVAKDENASDLLSLAALKEPLFSRKLVQLIKILSVFRL 501

Query: 433  RLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFR 492
              +MKCIIFVNRIV AR+LS IL NLE L  WK D+LVG+ S L+SMSR++M  IL +F+
Sbjct: 502  EPHMKCIIFVNRIVTARTLSCILNNLELLRSWKSDFLVGLSSGLKSMSRRSMETILKRFQ 561

Query: 493  SGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNE 552
            S ELNLLVATKVGEEGLDIQTCCLVIR+DLPETV+SFIQSRGRARMPQSEYAFLVDSGNE
Sbjct: 562  SKELNLLVATKVGEEGLDIQTCCLVIRYDLPETVTSFIQSRGRARMPQSEYAFLVDSGNE 621

Query: 553  KELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCS 612
            KE+ LI+ F+ +E+RM+ EI+ RSS E     +E +Y+V  TGA I+ G SISLL+++CS
Sbjct: 622  KEMDLIENFKVNEDRMNLEITYRSSEETCPRLDEELYKVHETGACISGGSSISLLYKYCS 681

Query: 613  KLPHDEYFDPKPKFFY--YDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAI 672
            +LPHDE+F PKP+F +   D+  GT+C   LP+NAPI +I S   PS E AKKDAC+KA+
Sbjct: 682  RLPHDEFFQPKPEFQFKPVDEFGGTICRITLPANAPISEIESSLLPSTEAAKKDACLKAV 741

Query: 673  EELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDE-TSRGELHEMLVPAALKDSWTG 732
             ELH LG LNDFLL    D  E+EL  D  D D  + E  SRG+L+EM VP   K  W  
Sbjct: 742  HELHNLGVLNDFLLPDSKDEIEDELSDDEFDFDNIKGEGCSRGDLYEMRVPVLFKQKWDP 801

Query: 733  SGYFI-LNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVPS 792
            S   + L+ Y I F PHP DRIYK+FG F+K+PLP EAE MDI+LHLA  RSV V + PS
Sbjct: 802  STSCVNLHSYYIMFVPHPADRIYKKFGFFMKSPLPVEAETMDIDLHLAHQRSVSVKIFPS 861

Query: 793  GVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLR-NNVSKSISSTSYLLLPLIFHD 852
            GV E   DEI  AE FQE+ LKV+ +R E + +F+PL   + S++  ST YLLLPL  HD
Sbjct: 862  GVTEFDNDEIRLAELFQEIALKVLFERGELIPDFVPLELQDSSRTSKSTFYLLLPLCLHD 921

Query: 853  NERSVSIDWKVIRRCLFSPIFQIPGSIVKET-ACSHAHLTLYDGRRRTSDIKNSLVYVPY 912
             E  +S+DW  IR CL SPIF+ P  +V++    S +HL L +G     D+KNSLV+  Y
Sbjct: 922  GESVISVDWVTIRNCLSSPIFKTPSVLVEDIFPPSGSHLKLANGCWNIDDVKNSLVFTTY 981

Query: 913  KREFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHN 972
             ++F+FV DI  G+NG+  S  K SS+ SH E + + +G+ LK+P QPLL  KPL H+ N
Sbjct: 982  SKQFYFVADICHGRNGF--SPVKESSTKSHVESIYKLYGVELKHPAQPLLRVKPLCHVRN 1041

Query: 973  LLHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIE 1032
            LLH+R Q N E   L EYFI++PPEL  LKI G SKDIGSS+SLLPSIMHR+ENLLVAIE
Sbjct: 1042 LLHNRMQTNLEPQELDEYFIEIPPELSHLKIKGLSKDIGSSLSLLPSIMHRMENLLVAIE 1101

Query: 1033 LKCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDK 1092
            LK +L+A+ P+ AEV+ +R+LEALTTEKC ER+SLERLE+LGD FLKFAV+R+LFL HD 
Sbjct: 1102 LKHVLSASIPEIAEVSGHRVLEALTTEKCHERLSLERLEVLGDAFLKFAVSRHLFLHHDS 1161

Query: 1093 SDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETRED 1152
             DEGELTRRRS +V N NL +LA +KNLQVYIRDQ  +P+QF+  G PC   C+E   ++
Sbjct: 1162 LDEGELTRRRSNVVNNSNLCRLAIKKNLQVYIRDQALDPTQFFAFGHPCRVTCDEVASKE 1221

Query: 1153 IHS--HCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIG 1212
            +HS         +   E +CS+GHHWL+KKTI+DVVEALVGAFLVDSGFK A+ FLKWIG
Sbjct: 1222 VHSLNRDLGILESNTGEIRCSKGHHWLYKKTIADVVEALVGAFLVDSGFKGAVKFLKWIG 1281

Query: 1213 IQVEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGG 1272
            + V+FE+  V +A ++S  Y+ L  R N+  L+N L ++FLHKGLL+QA +HPSY++HGG
Sbjct: 1282 VNVDFESLQVQDACIASRRYLPLTTRNNLETLENQLDYKFLHKGLLVQAFIHPSYNRHGG 1341

Query: 1273 GCYQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYL 1332
            GCYQRLEFLGDAVLDYL+TSY ++ +PKLKPG LTDLRS+ V NEA ANVAV  S  ++L
Sbjct: 1342 GCYQRLEFLGDAVLDYLMTSYFFTVFPKLKPGQLTDLRSLSVNNEALANVAVSFSLKRFL 1401

Query: 1333 ICDSTSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVW 1392
             C+S  L   I+ Y NF+ +    SG    P CPK LGDLVESC+GA+ +D GF++N+VW
Sbjct: 1402 FCESIYLHEVIEDYTNFLASSPLASGQSEGPRCPKVLGDLVESCLGALFLDCGFNLNHVW 1461

Query: 1393 KIMLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHAT 1452
             +MLSFLDP+ + S  Q+SPI+++IE CQ    + + + +K    ++VE +V  +    T
Sbjct: 1462 TMMLSFLDPVKNLSNLQISPIKELIELCQSYKWDREISATKKDGAFTVELKVTKNGCCLT 1521

Query: 1453 ASAAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTI 1512
             SA  R KR+  K AA L++T LKA   I   + LE++LK+  + E KLIGY+E P   +
Sbjct: 1522 VSATGRNKREGTKKAAQLMITNLKAHENITTSHPLEDVLKNGIRNEAKLIGYNEDPIDVV 1581

Query: 1513 DQADNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAF 1572
            D        +  LE+ G           GN+  +    I + L  +    +++L P  A 
Sbjct: 1582 DLVGLDVENLNILETFG-----------GNSERSSSYVIRRGLPQAPSKTEDRL-PQKAI 1641

Query: 1573 DAVSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEAPNTI 1632
              +  G     TA+S L+E C AN W PP F+C  EEGP HLK F YKV+LE+E+APN  
Sbjct: 1642 --IKAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMT 1687

Query: 1633 FKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYL 1650
             + +G     KK AAEHAA+AAIW LK +G+L
Sbjct: 1702 LECYGEARATKKGAAEHAAQAAIWCLKHSGFL 1687

BLAST of MS002366 vs. TAIR 10
Match: AT3G03300.1 (dicer-like 2 )

HSP 1 Score: 661.8 bits (1706), Expect = 1.5e-189
Identity = 472/1435 (32.89%), Postives = 746/1435 (51.99%), Query Frame = 0

Query: 49   PRRKARKYQLELCKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPT 108
            P   AR YQ+E  +KA+++N I++L TG GKT IA++L+    +L R+     CVFL P 
Sbjct: 20   PLHFARSYQVEALEKAIKQNTIVFLETGSGKTLIAIMLLRSYAYLFRKPSPCFCVFLVPQ 79

Query: 109  VALVQQQAKVIEDSTDFKVRVYCG--GSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHH 168
            V LV QQA+ ++  TD KV +Y G  G    +S   W++E++++EV VMTP ILL  L H
Sbjct: 80   VVLVTQQAEALKMHTDLKVGMYWGDMGVDFWDSS-TWKQEVDKYEVLVMTPAILLDALRH 139

Query: 169  CYIKMEFVELLIFDECHHAQVKSDHSYAEIMRVFY----KTNVAKRPRIFGMTASPVVGK 228
             ++ +  +++LI DECHHA  K  H YA IMR FY     +  +  PRIFGMTAS V  K
Sbjct: 140  SFLSLSMIKVLIVDECHHAGGK--HPYACIMREFYHKELNSGTSNVPRIFGMTASLVKTK 199

Query: 229  DHCSTSTNSGACNQQNLSKSINSLEKLLDAKVYSVEDREELDAFV--SSPLVKIYYYGPV 288
                        N  +  K I+ LE L+++KVY+ E+   L  FV  S+P  K Y +  +
Sbjct: 200  GE----------NLDSYWKKIHELETLMNSKVYTCENESVLAGFVPFSTPSFKYYQHIKI 259

Query: 289  ANGLSGSFMTYCSLLEDMKRQCI---VVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENL 348
             +          SL+E ++R  I   + LG +      + + +K L R+   + +CL++L
Sbjct: 260  PS------PKRASLVEKLERLTIKHRLSLGTLDLNSSTVDSVEKRLLRISSTLTYCLDDL 319

Query: 349  GVWGALQASKILLSGDNSERSELIEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSD 408
            G+  A +A++ L +  N    +     E N  + +L  ++ + A++        G   S 
Sbjct: 320  GILLAQKAAQSLSASQN----DSFLWGELNMFSVALVKKFCSDASQEFLAEIPQGLNWSV 379

Query: 409  TLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCIIFVNRIVIARSL-SYILQNLEFLAF 468
                   E    + K + LIE L  +    N++CIIFV+R++ A  L S + + L     
Sbjct: 380  ANINGNAEAGLLTLKTVCLIETLLGYSSLENIRCIIFVDRVITAIVLESLLAEILPNCNN 439

Query: 469  WKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLP 528
            WK  Y+ G +S L++ +RK  N I+  FR G +N++VAT + EEGLD+Q+C LVIRFD  
Sbjct: 440  WKTKYVAGNNSGLQNQTRKKQNEIVEDFRRGLVNIIVATSILEEGLDVQSCNLVIRFDPA 499

Query: 529  ETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLIDEFQKDENRMDREISSRS---SSEI 588
              + SFIQSRGRARM  S+Y  +V+SG+      + ++     RM  E    S      +
Sbjct: 500  SNICSFIQSRGRARMQNSDYLMMVESGDLLTQSRLMKYLSGGKRMREESLDHSLVPCPPL 559

Query: 589  FNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAI 648
             +  +E ++RV STGA++T   S+SL++ +CS+LP DEYF P P+F    D     C   
Sbjct: 560  PDDSDEPLFRVESTGATVTLSSSVSLIYHYCSRLPSDEYFKPAPRFDVNKDQGS--CTLY 619

Query: 649  LPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGALNDFLLLMRDDANEEELELDSS 708
            LP + P+ ++  + + + +  K+  C+KA  +LHK+GAL+D L+         ++ +  +
Sbjct: 620  LPKSCPVKEV--KAEANNKVLKQAVCLKACIQLHKVGALSDHLV--------PDMVVAET 679

Query: 709  DSDRSEDETSRGELHEMLVPAALKDSWTGSGYFILNCYQIKFSPH-PRDRIYKEFGLFVK 768
             S + E      E      P  L   ++       + Y I+  P+ PR+    +  L  +
Sbjct: 680  VSQKLEKIQYNTE-QPCYFPPELVSQFSAQPETTYHFYLIRMKPNSPRNFHLNDVLLGTR 739

Query: 769  APLPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEITQAESFQEMFLKVILDRSEFV 828
              L  +       L   RG ++ V L   G   L ++E+     FQ    +V+LD S  V
Sbjct: 740  VVLEDDIGNTSFRLEDHRG-TIAVTLSYVGAFHLTQEEVLFCRRFQITLFRVLLDHS--V 799

Query: 829  QEFIPLRNNVS-KSISSTSYLLLPLIFHDNERSVSIDWKVIRRCLFSPIFQIPGSIVKET 888
            +  +   N +  +   +  YLL+P   H +E S+ IDW+VIR    +    +       T
Sbjct: 800  ENLMEALNGLHLRDGVALDYLLVPST-HSHETSL-IDWEVIRSVNLTSHEVLEKHENCST 859

Query: 889  ACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKGKNGYSRSHYKNSSSLSHFE 948
              +   L   DG   T  ++N+LVY P+    +    ++   NG S    +NS   ++ E
Sbjct: 860  NGASRILHTKDGLFCTCVVQNALVYTPHNGYVYCTKGVLNNLNGNSLLTKRNSGDQTYIE 919

Query: 949  HLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEACYLQEYFIDLPPELCQLKII 1008
            +  ER GI L + ++PLL+ + +F LH+ LH  ++   +       F++LPPELC + + 
Sbjct: 920  YYEERHGIQLNFVDEPLLNGRHIFTLHSYLHMAKKKKEKE--HDREFVELPPELCHVILS 979

Query: 1009 GFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGAEVTANRILEALTTEKCQER 1068
              S D+  S + +PS+M R+E+LL+A  LK  +         +   ++LEA+TT+KC+++
Sbjct: 980  PISVDMIYSYTFIPSVMQRIESLLIAYNLKKSIPK-----VNIPTIKVLEAITTKKCEDQ 1039

Query: 1069 ISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYLVKNINLFKLATRKNLQVYI 1128
              LE LE LGD FLK+AV + LF       EG L+ ++  ++ N+ L +   ++ LQ +I
Sbjct: 1040 FHLESLETLGDSFLKYAVCQQLFQHCHTHHEGLLSTKKDGMISNVMLCQFGCQQKLQGFI 1099

Query: 1129 RDQPFEPSQFYTLGRPCP--RICNEETREDIHSHCNATKNAKASETKCSRGHHWLHKKTI 1188
            RD+ FEP  +   G+      + N+   E  + +  + +N K              +K++
Sbjct: 1100 RDECFEPKGWMVPGQSSAAYSLVNDTLPESRNIYVASRRNLK--------------RKSV 1159

Query: 1189 SDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALMSSNAYIILADRINISVL 1248
            +DVVE+L+GA+L + G  AA+ F+ W+GI+V+F     T   +  ++ I     +N+  +
Sbjct: 1160 ADVVESLIGAYLSEGGELAALMFMNWVGIKVDF-----TTTKIQRDSPIQAEKLVNVGYM 1219

Query: 1249 QNSLGHQFLHKGLLLQALVHPSY-HKHGGGCYQRLEFLGDAVLDYLITSYLYSAYPKLKP 1308
            ++ L + F  K LL++AL H SY       CYQRLEFLGD+VLDYLIT +LY  YP L P
Sbjct: 1220 ESLLNYSFEDKSLLVEALTHGSYMMPEIPRCYQRLEFLGDSVLDYLITKHLYDKYPCLSP 1279

Query: 1309 GLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYVNFIKAPS--SGSGSLG 1368
            GLLTD+RS  V NE +A VAV  + +K+++  S  L   I   V+  +  S  S  G   
Sbjct: 1280 GLLTDMRSASVNNECYALVAVKANLHKHILYASHHLHKHISRTVSEFEQSSLQSTFGWES 1339

Query: 1369 LPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSGFQLSPIRDIIEFCQ 1428
              S PK LGD++ES  GA+ VD+G++   V+  +   L  +I+    +L P+R++ E CQ
Sbjct: 1340 DISFPKVLGDVIESLAGAIFVDSGYNKEVVFASIKPLLGCMITPETVKLHPVRELTELCQ 1383

Query: 1429 DCGSNLQFNESKTGKYYSVEAEVKGDNFHATASAAHRRKRDAKKIAANLILTKLK 1462
                  QF  SK   + S   EVK        +A    K+ AKK+A   +L  LK
Sbjct: 1400 ----KWQFELSKAKDFDSFTVEVKAKEMSFAHTAKASDKKMAKKLAYKEVLNLLK 1383

BLAST of MS002366 vs. TAIR 10
Match: AT3G03300.3 (dicer-like 2 )

HSP 1 Score: 661.8 bits (1706), Expect = 1.5e-189
Identity = 472/1435 (32.89%), Postives = 746/1435 (51.99%), Query Frame = 0

Query: 49   PRRKARKYQLELCKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPT 108
            P   AR YQ+E  +KA+++N I++L TG GKT IA++L+    +L R+     CVFL P 
Sbjct: 20   PLHFARSYQVEALEKAIKQNTIVFLETGSGKTLIAIMLLRSYAYLFRKPSPCFCVFLVPQ 79

Query: 109  VALVQQQAKVIEDSTDFKVRVYCG--GSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHH 168
            V LV QQA+ ++  TD KV +Y G  G    +S   W++E++++EV VMTP ILL  L H
Sbjct: 80   VVLVTQQAEALKMHTDLKVGMYWGDMGVDFWDSS-TWKQEVDKYEVLVMTPAILLDALRH 139

Query: 169  CYIKMEFVELLIFDECHHAQVKSDHSYAEIMRVFY----KTNVAKRPRIFGMTASPVVGK 228
             ++ +  +++LI DECHHA  K  H YA IMR FY     +  +  PRIFGMTAS V  K
Sbjct: 140  SFLSLSMIKVLIVDECHHAGGK--HPYACIMREFYHKELNSGTSNVPRIFGMTASLVKTK 199

Query: 229  DHCSTSTNSGACNQQNLSKSINSLEKLLDAKVYSVEDREELDAFV--SSPLVKIYYYGPV 288
                        N  +  K I+ LE L+++KVY+ E+   L  FV  S+P  K Y +  +
Sbjct: 200  GE----------NLDSYWKKIHELETLMNSKVYTCENESVLAGFVPFSTPSFKYYQHIKI 259

Query: 289  ANGLSGSFMTYCSLLEDMKRQCI---VVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENL 348
             +          SL+E ++R  I   + LG +      + + +K L R+   + +CL++L
Sbjct: 260  PS------PKRASLVEKLERLTIKHRLSLGTLDLNSSTVDSVEKRLLRISSTLTYCLDDL 319

Query: 349  GVWGALQASKILLSGDNSERSELIEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSD 408
            G+  A +A++ L +  N    +     E N  + +L  ++ + A++        G   S 
Sbjct: 320  GILLAQKAAQSLSASQN----DSFLWGELNMFSVALVKKFCSDASQEFLAEIPQGLNWSV 379

Query: 409  TLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCIIFVNRIVIARSL-SYILQNLEFLAF 468
                   E    + K + LIE L  +    N++CIIFV+R++ A  L S + + L     
Sbjct: 380  ANINGNAEAGLLTLKTVCLIETLLGYSSLENIRCIIFVDRVITAIVLESLLAEILPNCNN 439

Query: 469  WKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLP 528
            WK  Y+ G +S L++ +RK  N I+  FR G +N++VAT + EEGLD+Q+C LVIRFD  
Sbjct: 440  WKTKYVAGNNSGLQNQTRKKQNEIVEDFRRGLVNIIVATSILEEGLDVQSCNLVIRFDPA 499

Query: 529  ETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLIDEFQKDENRMDREISSRS---SSEI 588
              + SFIQSRGRARM  S+Y  +V+SG+      + ++     RM  E    S      +
Sbjct: 500  SNICSFIQSRGRARMQNSDYLMMVESGDLLTQSRLMKYLSGGKRMREESLDHSLVPCPPL 559

Query: 589  FNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAI 648
             +  +E ++RV STGA++T   S+SL++ +CS+LP DEYF P P+F    D     C   
Sbjct: 560  PDDSDEPLFRVESTGATVTLSSSVSLIYHYCSRLPSDEYFKPAPRFDVNKDQGS--CTLY 619

Query: 649  LPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGALNDFLLLMRDDANEEELELDSS 708
            LP + P+ ++  + + + +  K+  C+KA  +LHK+GAL+D L+         ++ +  +
Sbjct: 620  LPKSCPVKEV--KAEANNKVLKQAVCLKACIQLHKVGALSDHLV--------PDMVVAET 679

Query: 709  DSDRSEDETSRGELHEMLVPAALKDSWTGSGYFILNCYQIKFSPH-PRDRIYKEFGLFVK 768
             S + E      E      P  L   ++       + Y I+  P+ PR+    +  L  +
Sbjct: 680  VSQKLEKIQYNTE-QPCYFPPELVSQFSAQPETTYHFYLIRMKPNSPRNFHLNDVLLGTR 739

Query: 769  APLPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEITQAESFQEMFLKVILDRSEFV 828
              L  +       L   RG ++ V L   G   L ++E+     FQ    +V+LD S  V
Sbjct: 740  VVLEDDIGNTSFRLEDHRG-TIAVTLSYVGAFHLTQEEVLFCRRFQITLFRVLLDHS--V 799

Query: 829  QEFIPLRNNVS-KSISSTSYLLLPLIFHDNERSVSIDWKVIRRCLFSPIFQIPGSIVKET 888
            +  +   N +  +   +  YLL+P   H +E S+ IDW+VIR    +    +       T
Sbjct: 800  ENLMEALNGLHLRDGVALDYLLVPST-HSHETSL-IDWEVIRSVNLTSHEVLEKHENCST 859

Query: 889  ACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKGKNGYSRSHYKNSSSLSHFE 948
              +   L   DG   T  ++N+LVY P+    +    ++   NG S    +NS   ++ E
Sbjct: 860  NGASRILHTKDGLFCTCVVQNALVYTPHNGYVYCTKGVLNNLNGNSLLTKRNSGDQTYIE 919

Query: 949  HLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEACYLQEYFIDLPPELCQLKII 1008
            +  ER GI L + ++PLL+ + +F LH+ LH  ++   +       F++LPPELC + + 
Sbjct: 920  YYEERHGIQLNFVDEPLLNGRHIFTLHSYLHMAKKKKEKE--HDREFVELPPELCHVILS 979

Query: 1009 GFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGAEVTANRILEALTTEKCQER 1068
              S D+  S + +PS+M R+E+LL+A  LK  +         +   ++LEA+TT+KC+++
Sbjct: 980  PISVDMIYSYTFIPSVMQRIESLLIAYNLKKSIPK-----VNIPTIKVLEAITTKKCEDQ 1039

Query: 1069 ISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYLVKNINLFKLATRKNLQVYI 1128
              LE LE LGD FLK+AV + LF       EG L+ ++  ++ N+ L +   ++ LQ +I
Sbjct: 1040 FHLESLETLGDSFLKYAVCQQLFQHCHTHHEGLLSTKKDGMISNVMLCQFGCQQKLQGFI 1099

Query: 1129 RDQPFEPSQFYTLGRPCP--RICNEETREDIHSHCNATKNAKASETKCSRGHHWLHKKTI 1188
            RD+ FEP  +   G+      + N+   E  + +  + +N K              +K++
Sbjct: 1100 RDECFEPKGWMVPGQSSAAYSLVNDTLPESRNIYVASRRNLK--------------RKSV 1159

Query: 1189 SDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALMSSNAYIILADRINISVL 1248
            +DVVE+L+GA+L + G  AA+ F+ W+GI+V+F     T   +  ++ I     +N+  +
Sbjct: 1160 ADVVESLIGAYLSEGGELAALMFMNWVGIKVDF-----TTTKIQRDSPIQAEKLVNVGYM 1219

Query: 1249 QNSLGHQFLHKGLLLQALVHPSY-HKHGGGCYQRLEFLGDAVLDYLITSYLYSAYPKLKP 1308
            ++ L + F  K LL++AL H SY       CYQRLEFLGD+VLDYLIT +LY  YP L P
Sbjct: 1220 ESLLNYSFEDKSLLVEALTHGSYMMPEIPRCYQRLEFLGDSVLDYLITKHLYDKYPCLSP 1279

Query: 1309 GLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYVNFIKAPS--SGSGSLG 1368
            GLLTD+RS  V NE +A VAV  + +K+++  S  L   I   V+  +  S  S  G   
Sbjct: 1280 GLLTDMRSASVNNECYALVAVKANLHKHILYASHHLHKHISRTVSEFEQSSLQSTFGWES 1339

Query: 1369 LPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSGFQLSPIRDIIEFCQ 1428
              S PK LGD++ES  GA+ VD+G++   V+  +   L  +I+    +L P+R++ E CQ
Sbjct: 1340 DISFPKVLGDVIESLAGAIFVDSGYNKEVVFASIKPLLGCMITPETVKLHPVRELTELCQ 1383

Query: 1429 DCGSNLQFNESKTGKYYSVEAEVKGDNFHATASAAHRRKRDAKKIAANLILTKLK 1462
                  QF  SK   + S   EVK        +A    K+ AKK+A   +L  LK
Sbjct: 1400 ----KWQFELSKAKDFDSFTVEVKAKEMSFAHTAKASDKKMAKKLAYKEVLNLLK 1383

BLAST of MS002366 vs. TAIR 10
Match: AT3G03300.2 (dicer-like 2 )

HSP 1 Score: 607.8 bits (1566), Expect = 2.5e-173
Identity = 443/1370 (32.34%), Postives = 705/1370 (51.46%), Query Frame = 0

Query: 114  QQAKVIEDSTDFKVRVYCG--GSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKM 173
            QQA+ ++  TD KV +Y G  G    +S   W++E++++EV VMTP ILL  L H ++ +
Sbjct: 71   QQAEALKMHTDLKVGMYWGDMGVDFWDSS-TWKQEVDKYEVLVMTPAILLDALRHSFLSL 130

Query: 174  EFVELLIFDECHHAQVKSDHSYAEIMRVFY----KTNVAKRPRIFGMTASPVVGKDHCST 233
              +++LI DECHHA  K  H YA IMR FY     +  +  PRIFGMTAS V  K     
Sbjct: 131  SMIKVLIVDECHHAGGK--HPYACIMREFYHKELNSGTSNVPRIFGMTASLVKTKGE--- 190

Query: 234  STNSGACNQQNLSKSINSLEKLLDAKVYSVEDREELDAFV--SSPLVKIYYYGPVANGLS 293
                   N  +  K I+ LE L+++KVY+ E+   L  FV  S+P  K Y +  + +   
Sbjct: 191  -------NLDSYWKKIHELETLMNSKVYTCENESVLAGFVPFSTPSFKYYQHIKIPS--- 250

Query: 294  GSFMTYCSLLEDMKRQCI---VVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGA 353
                   SL+E ++R  I   + LG +      + + +K L R+   + +CL++LG+  A
Sbjct: 251  ---PKRASLVEKLERLTIKHRLSLGTLDLNSSTVDSVEKRLLRISSTLTYCLDDLGILLA 310

Query: 354  LQASKILLSGDNSERSELIEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNAD 413
             +A++ L +  N    +     E N  + +L  ++ + A++        G   S      
Sbjct: 311  QKAAQSLSASQN----DSFLWGELNMFSVALVKKFCSDASQEFLAEIPQGLNWSVANING 370

Query: 414  ILEYPFFSKKLLQLIEILSSFRHRLNMKCIIFVNRIVIARSL-SYILQNLEFLAFWKCDY 473
              E    + K + LIE L  +    N++CIIFV+R++ A  L S + + L     WK  Y
Sbjct: 371  NAEAGLLTLKTVCLIETLLGYSSLENIRCIIFVDRVITAIVLESLLAEILPNCNNWKTKY 430

Query: 474  LVGVHSKLRSMSRKTMNHILMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSS 533
            + G +S L++ +RK  N I+  FR G +N++VAT + EEGLD+Q+C LVIRFD    + S
Sbjct: 431  VAGNNSGLQNQTRKKQNEIVEDFRRGLVNIIVATSILEEGLDVQSCNLVIRFDPASNICS 490

Query: 534  FIQSRGRARMPQSEYAFLVDSGNEKELGLIDEFQKDENRMDREISSRS---SSEIFNSHE 593
            FIQSRGRARM  S+Y  +V+SG+      + ++     RM  E    S      + +  +
Sbjct: 491  FIQSRGRARMQNSDYLMMVESGDLLTQSRLMKYLSGGKRMREESLDHSLVPCPPLPDDSD 550

Query: 594  ESIYRVASTGASITSGRSISLLHEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNA 653
            E ++RV STGA++T   S+SL++ +CS+LP DEYF P P+F    D     C   LP + 
Sbjct: 551  EPLFRVESTGATVTLSSSVSLIYHYCSRLPSDEYFKPAPRFDVNKDQGS--CTLYLPKSC 610

Query: 654  PIHQIISRTQPSREGAKKDACIKAIEELHKLGALNDFLLLMRDDANEEELELDSSDSDRS 713
            P+ ++  + + + +  K+  C+KA  +LHK+GAL+D L+         ++ +  + S + 
Sbjct: 611  PVKEV--KAEANNKVLKQAVCLKACIQLHKVGALSDHLV--------PDMVVAETVSQKL 670

Query: 714  EDETSRGELHEMLVPAALKDSWTGSGYFILNCYQIKFSPH-PRDRIYKEFGLFVKAPLPQ 773
            E      E      P  L   ++       + Y I+  P+ PR+    +  L  +  L  
Sbjct: 671  EKIQYNTE-QPCYFPPELVSQFSAQPETTYHFYLIRMKPNSPRNFHLNDVLLGTRVVLED 730

Query: 774  EAERMDIELHLARGRSVMVNLVPSGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIP 833
            +       L   RG ++ V L   G   L ++E+     FQ    +V+LD S  V+  + 
Sbjct: 731  DIGNTSFRLEDHRG-TIAVTLSYVGAFHLTQEEVLFCRRFQITLFRVLLDHS--VENLME 790

Query: 834  LRNNVS-KSISSTSYLLLPLIFHDNERSVSIDWKVIRRCLFSPIFQIPGSIVKETACSHA 893
              N +  +   +  YLL+P   H +E S+ IDW+VIR    +    +       T  +  
Sbjct: 791  ALNGLHLRDGVALDYLLVPST-HSHETSL-IDWEVIRSVNLTSHEVLEKHENCSTNGASR 850

Query: 894  HLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIER 953
             L   DG   T  ++N+LVY P+    +    ++   NG S    +NS   ++ E+  ER
Sbjct: 851  ILHTKDGLFCTCVVQNALVYTPHNGYVYCTKGVLNNLNGNSLLTKRNSGDQTYIEYYEER 910

Query: 954  FGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKD 1013
             GI L + ++PLL+ + +F LH+ LH  ++   +       F++LPPELC + +   S D
Sbjct: 911  HGIQLNFVDEPLLNGRHIFTLHSYLHMAKKKKEKE--HDREFVELPPELCHVILSPISVD 970

Query: 1014 IGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGAEVTANRILEALTTEKCQERISLER 1073
            +  S + +PS+M R+E+LL+A  LK  +         +   ++LEA+TT+KC+++  LE 
Sbjct: 971  MIYSYTFIPSVMQRIESLLIAYNLKKSIPK-----VNIPTIKVLEAITTKKCEDQFHLES 1030

Query: 1074 LEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPF 1133
            LE LGD FLK+AV + LF       EG L+ ++  ++ N+ L +   ++ LQ +IRD+ F
Sbjct: 1031 LETLGDSFLKYAVCQQLFQHCHTHHEGLLSTKKDGMISNVMLCQFGCQQKLQGFIRDECF 1090

Query: 1134 EPSQFYTLGRPCP--RICNEETREDIHSHCNATKNAKASETKCSRGHHWLHKKTISDVVE 1193
            EP  +   G+      + N+   E  + +  + +N K              +K+++DVVE
Sbjct: 1091 EPKGWMVPGQSSAAYSLVNDTLPESRNIYVASRRNLK--------------RKSVADVVE 1150

Query: 1194 ALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALMSSNAYIILADRINISVLQNSLG 1253
            +L+GA+L + G  AA+ F+ W+GI+V+F     T   +  ++ I     +N+  +++ L 
Sbjct: 1151 SLIGAYLSEGGELAALMFMNWVGIKVDF-----TTTKIQRDSPIQAEKLVNVGYMESLLN 1210

Query: 1254 HQFLHKGLLLQALVHPSY-HKHGGGCYQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTD 1313
            + F  K LL++AL H SY       CYQRLEFLGD+VLDYLIT +LY  YP L PGLLTD
Sbjct: 1211 YSFEDKSLLVEALTHGSYMMPEIPRCYQRLEFLGDSVLDYLITKHLYDKYPCLSPGLLTD 1270

Query: 1314 LRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYVNFIKAPS--SGSGSLGLPSCP 1373
            +RS  V NE +A VAV  + +K+++  S  L   I   V+  +  S  S  G     S P
Sbjct: 1271 MRSASVNNECYALVAVKANLHKHILYASHHLHKHISRTVSEFEQSSLQSTFGWESDISFP 1330

Query: 1374 KALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSGFQLSPIRDIIEFCQDCGSN 1433
            K LGD++ES  GA+ VD+G++   V+  +   L  +I+    +L P+R++ E CQ     
Sbjct: 1331 KVLGDVIESLAGAIFVDSGYNKEVVFASIKPLLGCMITPETVKLHPVRELTELCQ----K 1369

Query: 1434 LQFNESKTGKYYSVEAEVKGDNFHATASAAHRRKRDAKKIAANLILTKLK 1462
             QF  SK   + S   EVK        +A    K+ AKK+A   +L  LK
Sbjct: 1391 WQFELSKAKDFDSFTVEVKAKEMSFAHTAKASDKKMAKKLAYKEVLNLLK 1369

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134939.10.0e+0098.19dicer-like protein 4 isoform X1 [Momordica charantia][more]
XP_022134940.10.0e+0098.58dicer-like protein 4 isoform X2 [Momordica charantia][more]
XP_038879795.10.0e+0083.33dicer-like protein 4 isoform X2 [Benincasa hispida][more]
XP_038879794.10.0e+0083.34dicer-like protein 4 isoform X1 [Benincasa hispida][more]
XP_023515970.10.0e+0082.83dicer-like protein 4 isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
P846340.0e+0054.00Dicer-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=DCL4 PE=1 SV=2[more]
A7LFZ60.0e+0048.25Endoribonuclease Dicer homolog 4 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL... [more]
Q5N8701.0e-20331.23Endoribonuclease Dicer homolog 3a OS=Oryza sativa subsp. japonica OX=39947 GN=DC... [more]
Q3EBC82.1e-18832.89Endoribonuclease Dicer homolog 2 OS=Arabidopsis thaliana OX=3702 GN=At3g03300 PE... [more]
Q7XD961.4e-18131.37Endoribonuclease Dicer homolog 3b OS=Oryza sativa subsp. japonica OX=39947 GN=DC... [more]
Match NameE-valueIdentityDescription
A0A6J1C0500.0e+0098.19dicer-like protein 4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007071 P... [more]
A0A6J1BZQ80.0e+0098.58dicer-like protein 4 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007071 P... [more]
A0A6J1JAJ50.0e+0082.10dicer-like protein 4 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485046 PE=3... [more]
A0A6J1E1P40.0e+0082.36dicer-like protein 4 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429985 PE... [more]
A0A6J1JJM50.0e+0082.04dicer-like protein 4 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485046 PE=3... [more]
Match NameE-valueIdentityDescription
AT5G20320.10.0e+0054.00dicer-like 4 [more]
AT5G20320.20.0e+0053.39dicer-like 4 [more]
AT3G03300.11.5e-18932.89dicer-like 2 [more]
AT3G03300.31.5e-18932.89dicer-like 2 [more]
AT3G03300.22.5e-17332.34dicer-like 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001650Helicase, C-terminalSMARTSM00490helicmild6coord: 442..532
e-value: 2.1E-12
score: 57.2
IPR001650Helicase, C-terminalPFAMPF00271Helicase_Ccoord: 412..530
e-value: 8.3E-19
score: 68.0
IPR001650Helicase, C-terminalPROSITEPS51194HELICASE_CTERcoord: 412..585
score: 15.239359
IPR014720Double-stranded RNA-binding domainSMARTSM00358DRBM_3coord: 1570..1644
e-value: 1.8E-6
score: 37.5
coord: 1397..1461
e-value: 0.022
score: 20.9
IPR014720Double-stranded RNA-binding domainPROSITEPS50137DS_RBDcoord: 1396..1462
score: 8.930842
IPR014720Double-stranded RNA-binding domainPROSITEPS50137DS_RBDcoord: 1569..1645
score: 10.071829
IPR003100PAZ domainSMARTSM00949PAZ_2_a_3coord: 858..1016
e-value: 4.1E-11
score: 52.9
IPR003100PAZ domainPFAMPF02170PAZcoord: 884..1012
e-value: 4.3E-8
score: 33.1
IPR003100PAZ domainPROSITEPS50821PAZcoord: 858..990
score: 13.497351
IPR014001Helicase superfamily 1/2, ATP-binding domainSMARTSM00487ultradead3coord: 49..255
e-value: 3.1E-21
score: 86.5
IPR014001Helicase superfamily 1/2, ATP-binding domainPROSITEPS51192HELICASE_ATP_BIND_1coord: 60..224
score: 20.109943
IPR000999Ribonuclease III domainSMARTSM00535riboneu5coord: 1241..1393
e-value: 4.6E-31
score: 119.2
coord: 1037..1205
e-value: 1.6E-21
score: 87.5
IPR000999Ribonuclease III domainPFAMPF00636Ribonuclease_3coord: 1261..1370
e-value: 3.2E-20
score: 72.7
coord: 1055..1185
e-value: 3.0E-22
score: 79.2
IPR000999Ribonuclease III domainPROSITEPS00517RNASE_3_1coord: 1261..1269
IPR000999Ribonuclease III domainPROSITEPS50142RNASE_3_2coord: 1007..1185
score: 24.396465
IPR000999Ribonuclease III domainPROSITEPS50142RNASE_3_2coord: 1226..1370
score: 33.832542
IPR000999Ribonuclease III domainCDDcd00593RIBOccoord: 1242..1388
e-value: 2.08401E-36
score: 132.737
IPR000999Ribonuclease III domainCDDcd00593RIBOccoord: 1039..1195
e-value: 1.84557E-29
score: 112.706
NoneNo IPR availableGENE3D2.170.260.10paz domaincoord: 877..998
e-value: 4.4E-10
score: 41.4
NoneNo IPR availableGENE3D3.30.160.20coord: 1576..1646
e-value: 5.2E-21
score: 76.9
NoneNo IPR availablePFAMPF14709DND1_DSRMcoord: 1569..1644
e-value: 6.4E-14
score: 52.0
NoneNo IPR availablePFAMPF03368Dicer_dimercoord: 596..677
e-value: 3.2E-20
score: 72.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 32..51
NoneNo IPR availablePANTHERPTHR14950:SF15DICER-LIKE PROTEIN 4coord: 49..1646
NoneNo IPR availablePANTHERPTHR14950DICER-RELATEDcoord: 49..1646
NoneNo IPR availableCDDcd18802SF2_C_dicercoord: 405..540
e-value: 6.3754E-50
score: 171.62
NoneNo IPR availableCDDcd18034DEXHc_dicercoord: 52..259
e-value: 6.93525E-79
score: 256.81
NoneNo IPR availableSUPERFAMILY54768dsRNA-binding domain-likecoord: 1570..1650
NoneNo IPR availableSUPERFAMILY54768dsRNA-binding domain-likecoord: 1352..1462
IPR011545DEAD/DEAH box helicase domainPFAMPF00270DEADcoord: 55..216
e-value: 5.9E-15
score: 55.5
IPR038248Dicer dimerisation domain superfamilyGENE3D3.30.160.380Dicer dimerisation domaincoord: 591..689
e-value: 1.0E-23
score: 85.2
IPR036389Ribonuclease III, endonuclease domain superfamilyGENE3D1.10.1520.10Ribonuclease III domaincoord: 1035..1196
e-value: 5.4E-42
score: 145.5
coord: 1222..1391
e-value: 9.8E-48
score: 164.3
IPR036389Ribonuclease III, endonuclease domain superfamilySUPERFAMILY69065RNase III domain-likecoord: 1225..1388
IPR036389Ribonuclease III, endonuclease domain superfamilySUPERFAMILY69065RNase III domain-likecoord: 1038..1200
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 51..282
e-value: 8.8E-62
score: 210.6
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 408..567
e-value: 6.3E-38
score: 131.9
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 99..546
IPR005034Dicer dimerisation domainPROSITEPS51327DICER_DSRBFcoord: 596..686
score: 23.44194

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002366.1MS002366.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006396 RNA processing
molecular_function GO:0005524 ATP binding
molecular_function GO:0016891 endoribonuclease activity, producing 5'-phosphomonoesters
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0005515 protein binding
molecular_function GO:0004525 ribonuclease III activity