Homology
BLAST of MS002366 vs. NCBI nr
Match:
XP_022134939.1 (dicer-like protein 4 isoform X1 [Momordica charantia])
HSP 1 Score: 3233.0 bits (8381), Expect = 0.0e+00
Identity = 1631/1661 (98.19%), Postives = 1636/1661 (98.49%), Query Frame = 0
Query: 1 MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPSTRASEKDPRRKARKYQLEL 60
MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPS+RASEKDPRRKARKYQLEL
Sbjct: 2 MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPSSRASEKDPRRKARKYQLEL 61
Query: 61 CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE 120
CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE
Sbjct: 62 CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE 121
Query: 121 DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFD 180
DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLR LHHCYIKMEFVELLIFD
Sbjct: 122 DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRTLHHCYIKMEFVELLIFD 181
Query: 181 ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLS 240
ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGK GACNQQNLS
Sbjct: 182 ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGK---------GACNQQNLS 241
Query: 241 KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKR 300
KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKR
Sbjct: 242 KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKR 301
Query: 301 QCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSEL 360
QCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSEL
Sbjct: 302 QCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSEL 361
Query: 361 IEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEIL 420
IEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEIL
Sbjct: 362 IEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEIL 421
Query: 421 SSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHI 480
SSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHI
Sbjct: 422 SSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHI 481
Query: 481 LMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540
LMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV
Sbjct: 482 LMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 541
Query: 541 DSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLL 600
DSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLL
Sbjct: 542 DSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLL 601
Query: 601 HEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACI 660
HEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACI
Sbjct: 602 HEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACI 661
Query: 661 KAIEELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSW 720
KAIEELHKLGALNDFLL MRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSW
Sbjct: 662 KAIEELHKLGALNDFLLPMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSW 721
Query: 721 TGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVP 780
TGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKA LPQEAERMDIELHLARGRSVMVNLVP
Sbjct: 722 TGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAALPQEAERMDIELHLARGRSVMVNLVP 781
Query: 781 SGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHD 840
SGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHD
Sbjct: 782 SGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHD 841
Query: 841 NERSVSIDWKVIRRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYK 900
NERSVSIDWKVIRRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYK
Sbjct: 842 NERSVSIDWKVIRRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYK 901
Query: 901 REFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNL 960
REFFFVTDIVKGKNGYSRSHYKNSSS SHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNL
Sbjct: 902 REFFFVTDIVKGKNGYSRSHYKNSSSSSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNL 961
Query: 961 LHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIEL 1020
LHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIEL
Sbjct: 962 LHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIEL 1021
Query: 1021 KCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKS 1080
KCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKS
Sbjct: 1022 KCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKS 1081
Query: 1081 DEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDI 1140
DEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDI
Sbjct: 1082 DEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDI 1141
Query: 1141 HSHCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQV 1200
HSHCNATKNA+ASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQV
Sbjct: 1142 HSHCNATKNAQASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQV 1201
Query: 1201 EFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCY 1260
EFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCY
Sbjct: 1202 EFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCY 1261
Query: 1261 QRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICD 1320
QRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICD
Sbjct: 1262 QRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICD 1321
Query: 1321 STSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIM 1380
STSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIM
Sbjct: 1322 STSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIM 1381
Query: 1381 LSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHATASA 1440
LSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEA+VKGDNFHATASA
Sbjct: 1382 LSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEADVKGDNFHATASA 1441
Query: 1441 AHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTIDQA 1500
AHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVT+DQA
Sbjct: 1442 AHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTMDQA 1501
Query: 1501 DNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFDA- 1560
DNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFD
Sbjct: 1502 DNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFDGH 1561
Query: 1561 -------VSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEE 1620
S GRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEE
Sbjct: 1562 DSPTDVQSSSGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEE 1621
Query: 1621 APNTIFKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYLPETD 1654
AP+TIFKFFGAPHLRKKAAAEHAAEAAIWYLKK GYLPETD
Sbjct: 1622 APDTIFKFFGAPHLRKKAAAEHAAEAAIWYLKKEGYLPETD 1653
BLAST of MS002366 vs. NCBI nr
Match:
XP_022134940.1 (dicer-like protein 4 isoform X2 [Momordica charantia])
HSP 1 Score: 2755.3 bits (7141), Expect = 0.0e+00
Identity = 1389/1409 (98.58%), Postives = 1394/1409 (98.94%), Query Frame = 0
Query: 253 KVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKRQCIVVLGQMKGE 312
+VYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKRQCIVVLGQMKGE
Sbjct: 10 EVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKRQCIVVLGQMKGE 69
Query: 313 HEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNHS 372
HEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNHS
Sbjct: 70 HEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNHS 129
Query: 373 LSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCI 432
LSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCI
Sbjct: 130 LSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCI 189
Query: 433 IFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLL 492
IFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLL
Sbjct: 190 IFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLL 249
Query: 493 VATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLID 552
VATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLID
Sbjct: 250 VATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLID 309
Query: 553 EFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEY 612
EFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEY
Sbjct: 310 EFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEY 369
Query: 613 FDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGAL 672
FDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGAL
Sbjct: 370 FDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGAL 429
Query: 673 NDFLLLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSWTGSGYFILNCYQ 732
NDFLL MRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSWTGSGYFILNCYQ
Sbjct: 430 NDFLLPMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSWTGSGYFILNCYQ 489
Query: 733 IKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEIT 792
IKFSPHPRDRIYKEFGLFVKA LPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEIT
Sbjct: 490 IKFSPHPRDRIYKEFGLFVKAALPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEIT 549
Query: 793 QAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHDNERSVSIDWKVI 852
QAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHDNERSVSIDWKVI
Sbjct: 550 QAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHDNERSVSIDWKVI 609
Query: 853 RRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKG 912
RRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKG
Sbjct: 610 RRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKG 669
Query: 913 KNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEAC 972
KNGYSRSHYKNSSS SHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEAC
Sbjct: 670 KNGYSRSHYKNSSSSSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEAC 729
Query: 973 YLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGA 1032
YLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGA
Sbjct: 730 YLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGA 789
Query: 1033 EVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYL 1092
EVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYL
Sbjct: 790 EVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYL 849
Query: 1093 VKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDIHSHCNATKNAKA 1152
VKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDIHSHCNATKNA+A
Sbjct: 850 VKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDIHSHCNATKNAQA 909
Query: 1153 SETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALM 1212
SETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALM
Sbjct: 910 SETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALM 969
Query: 1213 SSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCYQRLEFLGDAVLD 1272
SSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCYQRLEFLGDAVLD
Sbjct: 970 SSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCYQRLEFLGDAVLD 1029
Query: 1273 YLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYV 1332
YLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYV
Sbjct: 1030 YLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYV 1089
Query: 1333 NFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSG 1392
NFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSG
Sbjct: 1090 NFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSG 1149
Query: 1393 FQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHATASAAHRRKRDAKKIA 1452
FQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEA+VKGDNFHATASAAHRRKRDAKKIA
Sbjct: 1150 FQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEADVKGDNFHATASAAHRRKRDAKKIA 1209
Query: 1453 ANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTIDQADNGFGTIKGLES 1512
ANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVT+DQADNGFGTIKGLES
Sbjct: 1210 ANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTMDQADNGFGTIKGLES 1269
Query: 1513 SGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFDA--------VSLGR 1572
SGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFD S GR
Sbjct: 1270 SGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFDGHDSPTDVQSSSGR 1329
Query: 1573 LCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEAPNTIFKFFGAP 1632
LCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEAP+TIFKFFGAP
Sbjct: 1330 LCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEAPDTIFKFFGAP 1389
Query: 1633 HLRKKAAAEHAAEAAIWYLKKAGYLPETD 1654
HLRKKAAAEHAAEAAIWYLKK GYLPETD
Sbjct: 1390 HLRKKAAAEHAAEAAIWYLKKEGYLPETD 1418
BLAST of MS002366 vs. NCBI nr
Match:
XP_038879795.1 (dicer-like protein 4 isoform X2 [Benincasa hispida])
HSP 1 Score: 2713.7 bits (7033), Expect = 0.0e+00
Identity = 1385/1662 (83.33%), Postives = 1484/1662 (89.29%), Query Frame = 0
Query: 1 MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPSTRASEKDPRRKARKYQLEL 60
MPDG+TNS DS NFQ T PVE F+GLS DDQ G STR SEKDPRR ARKYQLEL
Sbjct: 1 MPDGETNSTTDDSVNFQPTGFVPVERFMGLSQNDDQAAGSSTRTSEKDPRRIARKYQLEL 60
Query: 61 CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE 120
CKKALEENII+YLGTGCGKTHIA+LL+YEM HLIRRSQKGICVFLAPTVALVQQQAKVIE
Sbjct: 61 CKKALEENIIVYLGTGCGKTHIAILLIYEMSHLIRRSQKGICVFLAPTVALVQQQAKVIE 120
Query: 121 DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFD 180
DSTDFKVRVYCGGSKCLN+HYDWE EMEE+EVFVMTPEILLRNLHHCYIKM+FVELLIFD
Sbjct: 121 DSTDFKVRVYCGGSKCLNTHYDWETEMEEYEVFVMTPEILLRNLHHCYIKMDFVELLIFD 180
Query: 181 ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLS 240
ECHHAQVKSDHSYAEIMRVFYK N KRPRIFGMTASPVVGK GACNQQNLS
Sbjct: 181 ECHHAQVKSDHSYAEIMRVFYKANEPKRPRIFGMTASPVVGK---------GACNQQNLS 240
Query: 241 KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKR 300
+SINSLEKLLDAKVYSVE REELDAFV SPLVKIYYYGPVANG S SFMTYC LLED+K
Sbjct: 241 RSINSLEKLLDAKVYSVEAREELDAFVLSPLVKIYYYGPVANGSSSSFMTYCCLLEDIKH 300
Query: 301 QCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSEL 360
+CI+ LGQMK EHE L ATK+LLCRMH NIIFCLE+LGVWGALQASKILLSGDNSE+SEL
Sbjct: 301 KCILALGQMKCEHEVLLATKRLLCRMHKNIIFCLESLGVWGALQASKILLSGDNSEQSEL 360
Query: 361 IEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEIL 420
IEAAEGN N SLSD+YLNQAAE+ A GCKI GGMSD LN DILE PFFSKKLL+LI IL
Sbjct: 361 IEAAEGNLKNDSLSDKYLNQAAEVFASGCKIDGGMSDMLNVDILEDPFFSKKLLRLIGIL 420
Query: 421 SSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHI 480
SSFRHRLNMKCIIFVNRIVIARSLSYILQNL FL++WKCD+LVGVHSKLRSMSRKTM+HI
Sbjct: 421 SSFRHRLNMKCIIFVNRIVIARSLSYILQNLNFLSYWKCDFLVGVHSKLRSMSRKTMSHI 480
Query: 481 LMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540
L KF+SGELNLL+ATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV
Sbjct: 481 LTKFQSGELNLLIATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540
Query: 541 DSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLL 600
DSGNEKELGLIDEFQKDENRM+REISSRSS+E F SHEESIYRV STGASITSGRSISLL
Sbjct: 541 DSGNEKELGLIDEFQKDENRMNREISSRSSNETFCSHEESIYRVVSTGASITSGRSISLL 600
Query: 601 HEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACI 660
HE+CSKLPHDEYFDPKPKFFYYDDL GTVCHAILPSNAPI QI+S Q SR+ AKK AC+
Sbjct: 601 HEYCSKLPHDEYFDPKPKFFYYDDLEGTVCHAILPSNAPITQIVSGAQSSRDAAKKVACL 660
Query: 661 KAIEELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSW 720
KA+EELHKLGALND+LL MRD+A+E+ELELDSSDSD S+DE SR ELHEM+ PAALK+SW
Sbjct: 661 KAVEELHKLGALNDYLLPMRDNADEKELELDSSDSDSSKDENSRRELHEMIFPAALKESW 720
Query: 721 TGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVP 780
TGS Y +L CY IKF+PHPRDRIYKEFGLFVKAPLP+EAERMD+ELHLAR RSV VNL+P
Sbjct: 721 TGSEYLVLYCYHIKFTPHPRDRIYKEFGLFVKAPLPEEAERMDLELHLARRRSVRVNLMP 780
Query: 781 SGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHD 840
SGVVELLEDEI QAESFQEMFLKVILDRSEFVQE+IPLRNN S+SIS TSYLLLP+ FHD
Sbjct: 781 SGVVELLEDEIIQAESFQEMFLKVILDRSEFVQEYIPLRNNDSRSISPTSYLLLPVTFHD 840
Query: 841 NERSVSIDWKVIRRCLFSPIFQIPG-SIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPY 900
NE SV IDW+VI+RCL S IFQ SIVKE A S+ HLTLYDGRRR++DI+NSLVYVPY
Sbjct: 841 NEESVIIDWRVIKRCLSSQIFQNHACSIVKEAASSYTHLTLYDGRRRSNDIENSLVYVPY 900
Query: 901 KREFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHN 960
K EFFFVT+IV GKNGYS+ YKNS S SHFEHL +FGIHLK PEQPLL AKPLF LHN
Sbjct: 901 KGEFFFVTNIVWGKNGYSQ--YKNSGSSSHFEHLRSKFGIHLKSPEQPLLCAKPLFFLHN 960
Query: 961 LLHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIE 1020
LLH+R+Q +S+A YL+EYFIDLPPELCQLKI GFSKDIGSSISLLPSIMHRLENLLVAIE
Sbjct: 961 LLHNRKQEDSDAHYLEEYFIDLPPELCQLKICGFSKDIGSSISLLPSIMHRLENLLVAIE 1020
Query: 1021 LKCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDK 1080
LKC LAAAFP GAEVTANRILEALTTEKCQERISLERLEILGD FLKFAVARYLFLTHDK
Sbjct: 1021 LKCRLAAAFPAGAEVTANRILEALTTEKCQERISLERLEILGDSFLKFAVARYLFLTHDK 1080
Query: 1081 SDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETRED 1140
DEGELTRRRSYLVKN NL KLATRKNLQVYIRDQPFEPSQFY LGRPCPRICNEETR+D
Sbjct: 1081 FDEGELTRRRSYLVKNFNLLKLATRKNLQVYIRDQPFEPSQFYLLGRPCPRICNEETRKD 1140
Query: 1141 IHSHCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQ 1200
IHSH +AT N +ASETKCS+GHHWL KKTISDVVEALVGAFLVDSGFKAAI+FLKWIGIQ
Sbjct: 1141 IHSHDDAT-NTRASETKCSKGHHWLQKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIQ 1200
Query: 1201 VEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGC 1260
VEFE+SLVT+ALM+SN Y++LADR +IS Q+SLGHQFLHKGLLLQALVHPSYHKHGGGC
Sbjct: 1201 VEFESSLVTDALMASNNYVLLADRTDISAFQDSLGHQFLHKGLLLQALVHPSYHKHGGGC 1260
Query: 1261 YQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLIC 1320
YQRLEFLGDAVLDYLITSYLYSAYPKLKPG LTDLRSVFVRNEAFANV VDR FYK+L+C
Sbjct: 1261 YQRLEFLGDAVLDYLITSYLYSAYPKLKPGQLTDLRSVFVRNEAFANVTVDRFFYKFLLC 1320
Query: 1321 DSTSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKI 1380
DSTSL SDIKSYV+FIK+PS SL P CPKALGDLVES VGAVLVDTGFDMNYVWKI
Sbjct: 1321 DSTSLLSDIKSYVDFIKSPSE-RDSLEQPRCPKALGDLVESSVGAVLVDTGFDMNYVWKI 1380
Query: 1381 MLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHATAS 1440
MLSF+DPI+SFSGFQLSPIRD+IEFCQ+CG L+FN SK YYSV+AEVKG NFH TAS
Sbjct: 1381 MLSFVDPIMSFSGFQLSPIRDLIEFCQNCGWKLEFNSSKMEGYYSVKAEVKGGNFHDTAS 1440
Query: 1441 AAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTIDQ 1500
AA+RRK+DA+KIAANLILTKLKA+G +PEVNSLEEILKSS +MEPKLIGYDETP +DQ
Sbjct: 1441 AANRRKKDAEKIAANLILTKLKAKGFLPEVNSLEEILKSSSRMEPKLIGYDETPFGMVDQ 1500
Query: 1501 ADNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFDA 1560
DNG TI G E S ADL+P VH EV N+HP ITRIS+ VSSS AA EQL PS+A +
Sbjct: 1501 VDNGLETINGPEFSNADLNPRVHREVDNSHPVHITRISRTPVSSSVAAGEQLKPSMALEG 1560
Query: 1561 --------VSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIE 1620
S G K TARSRLYEICAANHW+ PSFDCMNEEGPSHLKMFTYKVVLE+E
Sbjct: 1561 HDSPADLQSSSGWSGKTTARSRLYEICAANHWNRPSFDCMNEEGPSHLKMFTYKVVLEME 1620
Query: 1621 EAPNTIFKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYLPETD 1654
EAP+TIF+F GAPHLRKKAAAEHAAEAA+WYLKKAGYLPETD
Sbjct: 1621 EAPDTIFEFVGAPHLRKKAAAEHAAEAALWYLKKAGYLPETD 1649
BLAST of MS002366 vs. NCBI nr
Match:
XP_038879794.1 (dicer-like protein 4 isoform X1 [Benincasa hispida])
HSP 1 Score: 2711.4 bits (7027), Expect = 0.0e+00
Identity = 1386/1663 (83.34%), Postives = 1485/1663 (89.30%), Query Frame = 0
Query: 1 MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPSTRASEKDPRRKARKYQLEL 60
MPDG+TNS DS NFQ T PVE F+GLS DDQ G STR SEKDPRR ARKYQLEL
Sbjct: 1 MPDGETNSTTDDSVNFQPTGFVPVERFMGLSQNDDQAAGSSTRTSEKDPRRIARKYQLEL 60
Query: 61 CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE 120
CKKALEENII+YLGTGCGKTHIA+LL+YEM HLIRRSQKGICVFLAPTVALVQQQAKVIE
Sbjct: 61 CKKALEENIIVYLGTGCGKTHIAILLIYEMSHLIRRSQKGICVFLAPTVALVQQQAKVIE 120
Query: 121 DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFD 180
DSTDFKVRVYCGGSKCLN+HYDWE EMEE+EVFVMTPEILLRNLHHCYIKM+FVELLIFD
Sbjct: 121 DSTDFKVRVYCGGSKCLNTHYDWETEMEEYEVFVMTPEILLRNLHHCYIKMDFVELLIFD 180
Query: 181 ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLS 240
ECHHAQVKSDHSYAEIMRVFYK N KRPRIFGMTASPVVGK GACNQQNLS
Sbjct: 181 ECHHAQVKSDHSYAEIMRVFYKANEPKRPRIFGMTASPVVGK---------GACNQQNLS 240
Query: 241 KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMK- 300
+SINSLEKLLDAKVYSVE REELDAFV SPLVKIYYYGPVANG S SFMTYC LLED+K
Sbjct: 241 RSINSLEKLLDAKVYSVEAREELDAFVLSPLVKIYYYGPVANGSSSSFMTYCCLLEDIKH 300
Query: 301 RQCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSE 360
+QCI+ LGQMK EHE L ATK+LLCRMH NIIFCLE+LGVWGALQASKILLSGDNSE+SE
Sbjct: 301 KQCILALGQMKCEHEVLLATKRLLCRMHKNIIFCLESLGVWGALQASKILLSGDNSEQSE 360
Query: 361 LIEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEI 420
LIEAAEGN N SLSD+YLNQAAE+ A GCKI GGMSD LN DILE PFFSKKLL+LI I
Sbjct: 361 LIEAAEGNLKNDSLSDKYLNQAAEVFASGCKIDGGMSDMLNVDILEDPFFSKKLLRLIGI 420
Query: 421 LSSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNH 480
LSSFRHRLNMKCIIFVNRIVIARSLSYILQNL FL++WKCD+LVGVHSKLRSMSRKTM+H
Sbjct: 421 LSSFRHRLNMKCIIFVNRIVIARSLSYILQNLNFLSYWKCDFLVGVHSKLRSMSRKTMSH 480
Query: 481 ILMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFL 540
IL KF+SGELNLL+ATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFL
Sbjct: 481 ILTKFQSGELNLLIATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFL 540
Query: 541 VDSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISL 600
VDSGNEKELGLIDEFQKDENRM+REISSRSS+E F SHEESIYRV STGASITSGRSISL
Sbjct: 541 VDSGNEKELGLIDEFQKDENRMNREISSRSSNETFCSHEESIYRVVSTGASITSGRSISL 600
Query: 601 LHEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDAC 660
LHE+CSKLPHDEYFDPKPKFFYYDDL GTVCHAILPSNAPI QI+S Q SR+ AKK AC
Sbjct: 601 LHEYCSKLPHDEYFDPKPKFFYYDDLEGTVCHAILPSNAPITQIVSGAQSSRDAAKKVAC 660
Query: 661 IKAIEELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDS 720
+KA+EELHKLGALND+LL MRD+A+E+ELELDSSDSD S+DE SR ELHEM+ PAALK+S
Sbjct: 661 LKAVEELHKLGALNDYLLPMRDNADEKELELDSSDSDSSKDENSRRELHEMIFPAALKES 720
Query: 721 WTGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLV 780
WTGS Y +L CY IKF+PHPRDRIYKEFGLFVKAPLP+EAERMD+ELHLAR RSV VNL+
Sbjct: 721 WTGSEYLVLYCYHIKFTPHPRDRIYKEFGLFVKAPLPEEAERMDLELHLARRRSVRVNLM 780
Query: 781 PSGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFH 840
PSGVVELLEDEI QAESFQEMFLKVILDRSEFVQE+IPLRNN S+SIS TSYLLLP+ FH
Sbjct: 781 PSGVVELLEDEIIQAESFQEMFLKVILDRSEFVQEYIPLRNNDSRSISPTSYLLLPVTFH 840
Query: 841 DNERSVSIDWKVIRRCLFSPIFQIPG-SIVKETACSHAHLTLYDGRRRTSDIKNSLVYVP 900
DNE SV IDW+VI+RCL S IFQ SIVKE A S+ HLTLYDGRRR++DI+NSLVYVP
Sbjct: 841 DNEESVIIDWRVIKRCLSSQIFQNHACSIVKEAASSYTHLTLYDGRRRSNDIENSLVYVP 900
Query: 901 YKREFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLH 960
YK EFFFVT+IV GKNGYS+ YKNS S SHFEHL +FGIHLK PEQPLL AKPLF LH
Sbjct: 901 YKGEFFFVTNIVWGKNGYSQ--YKNSGSSSHFEHLRSKFGIHLKSPEQPLLCAKPLFFLH 960
Query: 961 NLLHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAI 1020
NLLH+R+Q +S+A YL+EYFIDLPPELCQLKI GFSKDIGSSISLLPSIMHRLENLLVAI
Sbjct: 961 NLLHNRKQEDSDAHYLEEYFIDLPPELCQLKICGFSKDIGSSISLLPSIMHRLENLLVAI 1020
Query: 1021 ELKCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHD 1080
ELKC LAAAFP GAEVTANRILEALTTEKCQERISLERLEILGD FLKFAVARYLFLTHD
Sbjct: 1021 ELKCRLAAAFPAGAEVTANRILEALTTEKCQERISLERLEILGDSFLKFAVARYLFLTHD 1080
Query: 1081 KSDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETRE 1140
K DEGELTRRRSYLVKN NL KLATRKNLQVYIRDQPFEPSQFY LGRPCPRICNEETR+
Sbjct: 1081 KFDEGELTRRRSYLVKNFNLLKLATRKNLQVYIRDQPFEPSQFYLLGRPCPRICNEETRK 1140
Query: 1141 DIHSHCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGI 1200
DIHSH +AT N +ASETKCS+GHHWL KKTISDVVEALVGAFLVDSGFKAAI+FLKWIGI
Sbjct: 1141 DIHSHDDAT-NTRASETKCSKGHHWLQKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGI 1200
Query: 1201 QVEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGG 1260
QVEFE+SLVT+ALM+SN Y++LADR +IS Q+SLGHQFLHKGLLLQALVHPSYHKHGGG
Sbjct: 1201 QVEFESSLVTDALMASNNYVLLADRTDISAFQDSLGHQFLHKGLLLQALVHPSYHKHGGG 1260
Query: 1261 CYQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLI 1320
CYQRLEFLGDAVLDYLITSYLYSAYPKLKPG LTDLRSVFVRNEAFANV VDR FYK+L+
Sbjct: 1261 CYQRLEFLGDAVLDYLITSYLYSAYPKLKPGQLTDLRSVFVRNEAFANVTVDRFFYKFLL 1320
Query: 1321 CDSTSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWK 1380
CDSTSL SDIKSYV+FIK+PS SL P CPKALGDLVES VGAVLVDTGFDMNYVWK
Sbjct: 1321 CDSTSLLSDIKSYVDFIKSPSE-RDSLEQPRCPKALGDLVESSVGAVLVDTGFDMNYVWK 1380
Query: 1381 IMLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHATA 1440
IMLSF+DPI+SFSGFQLSPIRD+IEFCQ+CG L+FN SK YYSV+AEVKG NFH TA
Sbjct: 1381 IMLSFVDPIMSFSGFQLSPIRDLIEFCQNCGWKLEFNSSKMEGYYSVKAEVKGGNFHDTA 1440
Query: 1441 SAAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTID 1500
SAA+RRK+DA+KIAANLILTKLKA+G +PEVNSLEEILKSS +MEPKLIGYDETP +D
Sbjct: 1441 SAANRRKKDAEKIAANLILTKLKAKGFLPEVNSLEEILKSSSRMEPKLIGYDETPFGMVD 1500
Query: 1501 QADNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFD 1560
Q DNG TI G E S ADL+P VH EV N+HP ITRIS+ VSSS AA EQL PS+A +
Sbjct: 1501 QVDNGLETINGPEFSNADLNPRVHREVDNSHPVHITRISRTPVSSSVAAGEQLKPSMALE 1560
Query: 1561 A--------VSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEI 1620
S G K TARSRLYEICAANHW+ PSFDCMNEEGPSHLKMFTYKVVLE+
Sbjct: 1561 GHDSPADLQSSSGWSGKTTARSRLYEICAANHWNRPSFDCMNEEGPSHLKMFTYKVVLEM 1620
Query: 1621 EEAPNTIFKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYLPETD 1654
EEAP+TIF+F GAPHLRKKAAAEHAAEAA+WYLKKAGYLPETD
Sbjct: 1621 EEAPDTIFEFVGAPHLRKKAAAEHAAEAALWYLKKAGYLPETD 1650
BLAST of MS002366 vs. NCBI nr
Match:
XP_023515970.1 (dicer-like protein 4 isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 2693.7 bits (6981), Expect = 0.0e+00
Identity = 1385/1672 (82.83%), Postives = 1473/1672 (88.10%), Query Frame = 0
Query: 1 MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPSTRASEKDPRRKARKYQLEL 60
MPDG+TNS ADS NFQ TS VE F+GLS VDDQ G S R S KDPRR ARKYQLEL
Sbjct: 1 MPDGETNSTTADSVNFQPTSSASVERFMGLSPVDDQGAGSSIRTSGKDPRRIARKYQLEL 60
Query: 61 CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE 120
C+KALEENII+YLGTGCGKTHIA+LL+YEM HLIRR QKGICVFLAPTVALVQQQAKVIE
Sbjct: 61 CEKALEENIIVYLGTGCGKTHIAILLIYEMGHLIRRPQKGICVFLAPTVALVQQQAKVIE 120
Query: 121 DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFD 180
DS DFKV+VYCGGSKCLNSHYDWEREMEE+EVFVMTPEILLRNLHHCYIKMEFVELLIFD
Sbjct: 121 DSIDFKVKVYCGGSKCLNSHYDWEREMEEYEVFVMTPEILLRNLHHCYIKMEFVELLIFD 180
Query: 181 ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLS 240
ECHHAQVKSDHSYAEIMRVFYK N KRPRIFGMTASPVVGK GACNQQNLS
Sbjct: 181 ECHHAQVKSDHSYAEIMRVFYKANEHKRPRIFGMTASPVVGK---------GACNQQNLS 240
Query: 241 KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKR 300
KSINSLEKLLDAKVYSVEDREEL+AFVSSP KIYYYGPVA G S SFMTYC LED+K
Sbjct: 241 KSINSLEKLLDAKVYSVEDREELEAFVSSPRFKIYYYGPVATGSSSSFMTYCCPLEDVKC 300
Query: 301 QCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSEL 360
QCIV LGQMKGEHE L ATKKLLCRMH NIIFCLENLGVWGALQASKILLS DNSERSEL
Sbjct: 301 QCIVALGQMKGEHEGLLATKKLLCRMHKNIIFCLENLGVWGALQASKILLSEDNSERSEL 360
Query: 361 IEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEIL 420
IEAAE NP + S+SDRYLNQAAEI A GCKI G+SD LN DIL+ P FSKKLL+LI IL
Sbjct: 361 IEAAEANPKHDSISDRYLNQAAEIFASGCKIDAGVSDMLNVDILKEPCFSKKLLRLIGIL 420
Query: 421 SSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHI 480
SSFRHR NMKCIIFVNRIVIARSLSYILQNL+FLA+WKCD+LVGVHSKLRSMSRKTMNHI
Sbjct: 421 SSFRHRPNMKCIIFVNRIVIARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNHI 480
Query: 481 LMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540
L KFRSGELNLL+ATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV
Sbjct: 481 LTKFRSGELNLLIATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540
Query: 541 DSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLL 600
DSGNEKELGLIDEF KDE RM+REISSRSS E F+ HEESIYRV STGAS+TSGRSISLL
Sbjct: 541 DSGNEKELGLIDEFHKDEKRMNREISSRSSIETFDGHEESIYRVVSTGASVTSGRSISLL 600
Query: 601 HEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACI 660
HE+CSKLPHD YFDPKPKFFY DDL GT+CHAILPSNAPI QI+SR QP+ AKKDAC+
Sbjct: 601 HEYCSKLPHDTYFDPKPKFFYCDDLGGTICHAILPSNAPIPQIVSRPQPAGVAAKKDACL 660
Query: 661 KAIEELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSW 720
KA+EELHKLGAL+D+LL MRD+ANE ELELD SDSD SEDETSR ELHEM+ PAA K+SW
Sbjct: 661 KAVEELHKLGALSDYLLPMRDNANEGELELD-SDSDSSEDETSRRELHEMISPAAFKESW 720
Query: 721 TGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVP 780
TG GY IL CY IKF+P+P DRIYKEFGLFVKAPLPQEA RMD+ELHLA GRSV VNL+P
Sbjct: 721 TGFGYVILYCYHIKFTPNPEDRIYKEFGLFVKAPLPQEAVRMDLELHLAHGRSVKVNLMP 780
Query: 781 SGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHD 840
S VVELLE+EITQAE FQEMFLKVILDRSEFV ++IPLRNNVS+SISSTSYLLLP+I HD
Sbjct: 781 SRVVELLENEITQAECFQEMFLKVILDRSEFVHDYIPLRNNVSRSISSTSYLLLPVIVHD 840
Query: 841 NERSVSIDWKVIRRCLFSPIFQIPG-SIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPY 900
NE SV IDWKVIRRCL S IFQ SIVKETA S HL LYDGRRR+SDI+NSLVYVPY
Sbjct: 841 NEGSVFIDWKVIRRCLSSQIFQNHACSIVKETASSDTHLMLYDGRRRSSDIENSLVYVPY 900
Query: 901 KREFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHN 960
KREFFFVT+IV+ KNGYS+ YK+S SLSHFEHL RFGIHLKYPEQPLL AKPLF LHN
Sbjct: 901 KREFFFVTNIVRRKNGYSQ--YKDSGSLSHFEHLKTRFGIHLKYPEQPLLCAKPLFSLHN 960
Query: 961 LLHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIE 1020
LLH+R+Q +SEA YL+EYFID+ PELCQLKI GFSKDIGSSISLLPSIMHRLENLLVAIE
Sbjct: 961 LLHNRKQKDSEAPYLEEYFIDIAPELCQLKITGFSKDIGSSISLLPSIMHRLENLLVAIE 1020
Query: 1021 LKCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDK 1080
LKCMLAAAFP GAEVTANRILEALTTEKCQERISLERLEILGD FLKFAVARYLFL HDK
Sbjct: 1021 LKCMLAAAFPAGAEVTANRILEALTTEKCQERISLERLEILGDSFLKFAVARYLFLAHDK 1080
Query: 1081 SDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETRED 1140
DEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFY LGRPCPRICNEETR D
Sbjct: 1081 FDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYLLGRPCPRICNEETRND 1140
Query: 1141 IHSHCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQ 1200
IHSH NAT NAKASETKCS+GHHWL KKTISDVVEALVGAFLVDSGFKAAI+FLKWIGI+
Sbjct: 1141 IHSHDNATNNAKASETKCSKGHHWLQKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIK 1200
Query: 1201 VEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGC 1260
VEFEAS VTNAL++S AY++LAD INIS LQ+SLGH+FLHKGLLLQALVHP+YHKHGGGC
Sbjct: 1201 VEFEASQVTNALVASKAYVLLADTINISSLQSSLGHKFLHKGLLLQALVHPTYHKHGGGC 1260
Query: 1261 YQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLIC 1320
YQRLEFLGDAVLDYLI SYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDR FYK+L+C
Sbjct: 1261 YQRLEFLGDAVLDYLIMSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRFFYKFLLC 1320
Query: 1321 DSTSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKI 1380
DS SLQSDIKSYV+FIKAP S SL P CPK LGDLVES VGAVLVDTGFDMNYVWKI
Sbjct: 1321 DSASLQSDIKSYVDFIKAPPSERDSLEQPRCPKPLGDLVESSVGAVLVDTGFDMNYVWKI 1380
Query: 1381 MLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHATAS 1440
MLSFLDPI+S SGFQLSPIRDIIE CQ CG LQFN SK KYYSVEAEVKGD+FHATAS
Sbjct: 1381 MLSFLDPIMSSSGFQLSPIRDIIEVCQTCGWKLQFNPSKMEKYYSVEAEVKGDDFHATAS 1440
Query: 1441 AAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTIDQ 1500
A +RRK+DA+KIAAN ILTKLKAQG IPEVNSLE+ILKSS KM+PKLIGYDETPSVT+D+
Sbjct: 1441 AVNRRKKDAEKIAANSILTKLKAQGFIPEVNSLEKILKSSEKMKPKLIGYDETPSVTMDR 1500
Query: 1501 ADNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFD- 1560
D+ GLE SGADLDP VH EVG++ P RITR+S+ L SSSGAA EQL PS+ +
Sbjct: 1501 IDD------GLEFSGADLDPRVHREVGDSRPFRITRMSETLASSSGAAGEQLKPSIPLEG 1560
Query: 1561 -----------------AVSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKM 1620
+ S GR K TARSRLYE+CA NHW+ PSFDCMNEEGPSHLKM
Sbjct: 1561 HDSSTDLQSLSSSSSSSSSSSGRSGKTTARSRLYEVCAINHWNHPSFDCMNEEGPSHLKM 1620
Query: 1621 FTYKVVLEIEEAPNTIFKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYLPETD 1654
FTYKVVLEIEEAPNTIF+FFGAPHL KKAAAEHAAEAA+WYLKK YL +TD
Sbjct: 1621 FTYKVVLEIEEAPNTIFEFFGAPHLTKKAAAEHAAEAALWYLKKERYLGDTD 1654
BLAST of MS002366 vs. ExPASy Swiss-Prot
Match:
P84634 (Dicer-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=DCL4 PE=1 SV=2)
HSP 1 Score: 1682.9 bits (4357), Expect = 0.0e+00
Identity = 892/1652 (54.00%), Postives = 1145/1652 (69.31%), Query Frame = 0
Query: 13 SDNFQHTSLGPVESFVG-----LSLVDDQEPGPSTRASEKDPRRKARKYQLELCKKALEE 72
S + TS V SF S +P P EKDPR+ AR+YQ+ELCKKA EE
Sbjct: 82 SSDLHLTSSSSVSSFSSSSSSLFSAAGTDDPSPK---MEKDPRKIARRYQVELCKKATEE 141
Query: 73 NIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIEDSTDFKV 132
N+I+YLGTGCGKTHIAV+L+YE+ HL+ +K +C+FLAPTVALV+QQAKVI DS +FKV
Sbjct: 142 NVIVYLGTGCGKTHIAVMLIYELGHLVLSPKKSVCIFLAPTVALVEQQAKVIADSVNFKV 201
Query: 133 RVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFDECHHAQV 192
++CGG + + SH +WERE+ +EV VMTP+ILL NL HC+IKME + LLIFDECHHAQ
Sbjct: 202 AIHCGGKRIVKSHSEWEREIAANEVLVMTPQILLHNLQHCFIKMECISLLIFDECHHAQQ 261
Query: 193 KSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLSKSINSLE 252
+S+H YAEIM+VFYK+ +RPRIFGMTASPVVGK G+ +NLSKSINSLE
Sbjct: 262 QSNHPYAEIMKVFYKSESLQRPRIFGMTASPVVGK---------GSFQSENLSKSINSLE 321
Query: 253 KLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKRQCIVVLG 312
LL+AKVYSVE +LD FVSSPLVK+YYY + S S + Y ++LED+K++C+ L
Sbjct: 322 NLLNAKVYSVESNVQLDGFVSSPLVKVYYYRSALSDASQSTIRYENMLEDIKQRCLASLK 381
Query: 313 QMKGEH--EDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAE 372
+ H + L + K+LL R HDN+I+ L NLG+WGA+QA+KI L+ D++ + E +
Sbjct: 382 LLIDTHQTQTLLSMKRLLKRSHDNLIYTLLNLGLWGAIQAAKIQLNSDHNVQDEPV---- 441
Query: 373 GNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRH 432
G + D YL+ AAE L+ G SD L+ L+ P FS+KL+QLI+ILS FR
Sbjct: 442 GKNPKSKICDTYLSMAAEALSSGVAKDENASDLLSLAALKEPLFSRKLVQLIKILSVFRL 501
Query: 433 RLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFR 492
+MKCIIFVNRIV AR+LS IL NLE L WK D+LVG+ S L+SMSR++M IL +F+
Sbjct: 502 EPHMKCIIFVNRIVTARTLSCILNNLELLRSWKSDFLVGLSSGLKSMSRRSMETILKRFQ 561
Query: 493 SGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNE 552
S ELNLLVATKVGEEGLDIQTCCLVIR+DLPETV+SFIQSRGRARMPQSEYAFLVDSGNE
Sbjct: 562 SKELNLLVATKVGEEGLDIQTCCLVIRYDLPETVTSFIQSRGRARMPQSEYAFLVDSGNE 621
Query: 553 KELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCS 612
KE+ LI+ F+ +E+RM+ EI+ RSS E +E +Y+V TGA I+ G SISLL+++CS
Sbjct: 622 KEMDLIENFKVNEDRMNLEITYRSSEETCPRLDEELYKVHETGACISGGSSISLLYKYCS 681
Query: 613 KLPHDEYFDPKPKFFY--YDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAI 672
+LPHDE+F PKP+F + D+ GT+C LP+NAPI +I S PS E AKKDAC+KA+
Sbjct: 682 RLPHDEFFQPKPEFQFKPVDEFGGTICRITLPANAPISEIESSLLPSTEAAKKDACLKAV 741
Query: 673 EELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDE-TSRGELHEMLVPAALKDSWTG 732
ELH LG LNDFLL D E+EL D D D + E SRG+L+EM VP K W
Sbjct: 742 HELHNLGVLNDFLLPDSKDEIEDELSDDEFDFDNIKGEGCSRGDLYEMRVPVLFKQKWDP 801
Query: 733 SGYFI-LNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVPS 792
S + L+ Y I F PHP DRIYK+FG F+K+PLP EAE MDI+LHLA RSV V + PS
Sbjct: 802 STSCVNLHSYYIMFVPHPADRIYKKFGFFMKSPLPVEAETMDIDLHLAHQRSVSVKIFPS 861
Query: 793 GVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLR-NNVSKSISSTSYLLLPLIFHD 852
GV E DEI AE FQE+ LKV+ +R E + +F+PL + S++ ST YLLLPL HD
Sbjct: 862 GVTEFDNDEIRLAELFQEIALKVLFERGELIPDFVPLELQDSSRTSKSTFYLLLPLCLHD 921
Query: 853 NERSVSIDWKVIRRCLFSPIFQIPGSIVKET-ACSHAHLTLYDGRRRTSDIKNSLVYVPY 912
E +S+DW IR CL SPIF+ P +V++ S +HL L +G D+KNSLV+ Y
Sbjct: 922 GESVISVDWVTIRNCLSSPIFKTPSVLVEDIFPPSGSHLKLANGCWNIDDVKNSLVFTTY 981
Query: 913 KREFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHN 972
++F+FV DI G+NG+ S K SS+ SH E + + +G+ LK+P QPLL KPL H+ N
Sbjct: 982 SKQFYFVADICHGRNGF--SPVKESSTKSHVESIYKLYGVELKHPAQPLLRVKPLCHVRN 1041
Query: 973 LLHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIE 1032
LLH+R Q N E L EYFI++PPEL LKI G SKDIGSS+SLLPSIMHR+ENLLVAIE
Sbjct: 1042 LLHNRMQTNLEPQELDEYFIEIPPELSHLKIKGLSKDIGSSLSLLPSIMHRMENLLVAIE 1101
Query: 1033 LKCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDK 1092
LK +L+A+ P+ AEV+ +R+LEALTTEKC ER+SLERLE+LGD FLKFAV+R+LFL HD
Sbjct: 1102 LKHVLSASIPEIAEVSGHRVLEALTTEKCHERLSLERLEVLGDAFLKFAVSRHLFLHHDS 1161
Query: 1093 SDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETRED 1152
DEGELTRRRS +V N NL +LA +KNLQVYIRDQ +P+QF+ G PC C+E ++
Sbjct: 1162 LDEGELTRRRSNVVNNSNLCRLAIKKNLQVYIRDQALDPTQFFAFGHPCRVTCDEVASKE 1221
Query: 1153 IHS--HCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIG 1212
+HS + E +CS+GHHWL+KKTI+DVVEALVGAFLVDSGFK A+ FLKWIG
Sbjct: 1222 VHSLNRDLGILESNTGEIRCSKGHHWLYKKTIADVVEALVGAFLVDSGFKGAVKFLKWIG 1281
Query: 1213 IQVEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGG 1272
+ V+FE+ V +A ++S Y+ L R N+ L+N L ++FLHKGLL+QA +HPSY++HGG
Sbjct: 1282 VNVDFESLQVQDACIASRRYLPLTTRNNLETLENQLDYKFLHKGLLVQAFIHPSYNRHGG 1341
Query: 1273 GCYQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYL 1332
GCYQRLEFLGDAVLDYL+TSY ++ +PKLKPG LTDLRS+ V NEA ANVAV S ++L
Sbjct: 1342 GCYQRLEFLGDAVLDYLMTSYFFTVFPKLKPGQLTDLRSLSVNNEALANVAVSFSLKRFL 1401
Query: 1333 ICDSTSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVW 1392
C+S L I+ Y NF+ + SG P CPK LGDLVESC+GA+ +D GF++N+VW
Sbjct: 1402 FCESIYLHEVIEDYTNFLASSPLASGQSEGPRCPKVLGDLVESCLGALFLDCGFNLNHVW 1461
Query: 1393 KIMLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHAT 1452
+MLSFLDP+ + S Q+SPI+++IE CQ + + + +K ++VE +V + T
Sbjct: 1462 TMMLSFLDPVKNLSNLQISPIKELIELCQSYKWDREISATKKDGAFTVELKVTKNGCCLT 1521
Query: 1453 ASAAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTI 1512
SA R KR+ K AA L++T LKA I + LE++LK+ + E KLIGY+E P +
Sbjct: 1522 VSATGRNKREGTKKAAQLMITNLKAHENITTSHPLEDVLKNGIRNEAKLIGYNEDPIDVV 1581
Query: 1513 DQADNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAF 1572
D + LE+ G GN+ + I + L + +++L P A
Sbjct: 1582 DLVGLDVENLNILETFG-----------GNSERSSSYVIRRGLPQAPSKTEDRL-PQKAI 1641
Query: 1573 DAVSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEAPNTI 1632
+ G TA+S L+E C AN W PP F+C EEGP HLK F YKV+LE+E+APN
Sbjct: 1642 --IKAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMT 1701
Query: 1633 FKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYL 1650
+ +G KK AAEHAA+AAIW LK +G+L
Sbjct: 1702 LECYGEARATKKGAAEHAAQAAIWCLKHSGFL 1701
BLAST of MS002366 vs. ExPASy Swiss-Prot
Match:
A7LFZ6 (Endoribonuclease Dicer homolog 4 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL4 PE=2 SV=1)
HSP 1 Score: 1441.8 bits (3731), Expect = 0.0e+00
Identity = 801/1660 (48.25%), Postives = 1083/1660 (65.24%), Query Frame = 0
Query: 39 GP-STRASEKDPRRKARKYQLELCKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRS 98
GP STR KDPR ARKYQL+LCK+A+EENII+YLGTGCGKTHIAVLL+YE+ HLIR+
Sbjct: 15 GPSSTRGEPKDPRTIARKYQLDLCKRAVEENIIVYLGTGCGKTHIAVLLIYELGHLIRKP 74
Query: 99 QKGICVFLAPTVALVQQQAKVIEDSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTP 158
+ +C+FLAPT+ LV+QQA VI STDFKV+ Y G K H +WE +M E EV VMTP
Sbjct: 75 SREVCIFLAPTIPLVRQQAVVIASSTDFKVQCYYGNGKNSRDHQEWENDMREFEVLVMTP 134
Query: 159 EILLRNLHHCYIKMEFVELLIFDECHHAQVKSDHSYAEIMRVFYKTN-VAKRPRIFGMTA 218
+ILL++L HC+IKM + LLI DECHHAQ + H YA+IM+ FY +N V K PR+FGMTA
Sbjct: 135 QILLQSLRHCFIKMNSIALLILDECHHAQPQKRHPYAQIMKEFYNSNSVEKFPRVFGMTA 194
Query: 219 SPVVGK----DHCSTSTN-------------SGACNQQNLSKSINSLEKLLDAKVYSVED 278
SP++GK H T G N+ N +K INSLE+LL AKV SV D
Sbjct: 195 SPIIGKGVMPSHSFTEKGGRSPCQPLIFFLPKGGSNKLNYTKCINSLEELLHAKVCSV-D 254
Query: 279 REELDAFVSSPLVKIYYYGPVANGLSGSFMTYC-SLLEDMKRQCIVVLGQMKGEHEDLRA 338
EEL++ V+SP +++Y+YGPV + + T C L+ +K Q +L + +D +
Sbjct: 255 NEELESVVASPDMEVYFYGPVNH---SNLTTICIKELDSLKLQSERMLRASLCDFKDSQK 314
Query: 339 TKKLLCRMHDNIIFCLENLGVWGALQASKILLS--GDNSERSELIEAAEGNPNNHSLSDR 398
K L R+H+NIIFCL+ LG +GALQA++ LS GD +R E+ + N + S +
Sbjct: 315 KLKSLWRLHENIIFCLQELGSFGALQAARTFLSFDGDKLDRREV----DLNGSTSSFAHH 374
Query: 399 YLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCIIFVN 458
YLN A IL++ K G + + + + LE PFFS K LI +LS + + NMKCI+FV
Sbjct: 375 YLNGATSILSRN-KTDGSHAGSFDLEKLEEPFFSNKFSVLINVLSRYGLQENMKCIVFVK 434
Query: 459 RIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLLVATK 518
RI +AR++S ILQNL+ L FWKC++LVG HS ++MSR M+ I+ +F SGE+NLLVAT
Sbjct: 435 RITVARAISNILQNLKCLEFWKCEFLVGCHSGSKNMSRNKMDAIVQRFSSGEVNLLVATS 494
Query: 519 VGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLIDEFQK 578
VGEEGLDIQTCCLV+RFDLPETV+SFIQSRGRARM +S+Y L++ N+ L++ +
Sbjct: 495 VGEEGLDIQTCCLVVRFDLPETVASFIQSRGRARMTKSKYVVLLERENQSHEKLLNGYIA 554
Query: 579 DENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEYFDPK 638
E+ M+ EI SR+S+++F+ EE+IY+V +TGASI++ S+SLLH +C LP D +F P
Sbjct: 555 GESIMNEEIDSRTSNDMFDCLEENIYQVDNTGASISTACSVSLLHCYCDNLPRDMFFTPS 614
Query: 639 PKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGALNDFL 698
P FFY D + G +C ILP NA Q + S++ AK+DAC+KA +LHKLGAL DFL
Sbjct: 615 PVFFYIDGIEGIICRLILPPNAAFRQADGQPCLSKDEAKRDACLKACVKLHKLGALTDFL 674
Query: 699 LLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSWTG-SGYFILNCYQIKF 758
L + ++S +++ ED++ R ELHEML+PA LK S L+ Y +KF
Sbjct: 675 LPGPGSRKNKVSVTNNSSNNKVEDDSLREELHEMLIPAVLKPSGLKLDSLSNLHFYYVKF 734
Query: 759 SPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEITQAE 818
P P DR Y+ FGLFV PLP EAE + ++LHLARGR V + G + ++++ A
Sbjct: 735 IPIPEDRRYQMFGLFVINPLPVEAETLQVDLHLARGRIVKAGIKHLGKIAFEKEKMMLAH 794
Query: 819 SFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHDNERSVSIDWKVIRRC 878
FQEM LK++LDRSEF + L N+V+ I+ST YLLLP+ IDW + RC
Sbjct: 795 KFQEMCLKILLDRSEFTSPHVKLGNDVTLEINSTFYLLLPIKQKCYGDRFMIDWPAVERC 854
Query: 879 LFSPIFQIPGSI-VKETACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKGKN 938
L SPIF+ P + V + S+ L L DG +D+ S+V+ P+ FFFV I+ N
Sbjct: 855 LSSPIFKDPIDVSVHASYSSNESLRLLDGIFSKTDVVGSVVFSPHNNIFFFVDGILDEIN 914
Query: 939 GYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDR--RQGNSEAC 998
+S S ++ EH ERF I L +PEQPLL AK +F+L NLLH+R SE
Sbjct: 915 AWS-----EHSGATYAEHFKERFRIELSHPEQPLLKAKQIFNLRNLLHNRLPETTESEGR 974
Query: 999 YLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGA 1058
L E+F++LPPELC LK+IGFSKD+GSS+SLLPS+M+RLENLLVAIELK ++ ++FP+ +
Sbjct: 975 ELLEHFVELPPELCSLKVIGFSKDMGSSLSLLPSLMYRLENLLVAIELKDVMLSSFPEAS 1034
Query: 1059 EVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYL 1118
+++A+ ILEALTTEKC ERISLER E+LGD FLK+ V R+ F+T++ DEG+LTRRRS +
Sbjct: 1035 QISASGILEALTTEKCLERISLERFEVLGDAFLKYVVGRHKFITYEGLDEGQLTRRRSDV 1094
Query: 1119 VKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDIHSHCNATKNAKA 1178
V N +L++L+ RK LQVYIRDQ FEP+QF+ GRPC +CN + +H N +
Sbjct: 1095 VNNSHLYELSIRKKLQVYIRDQQFEPTQFFAPGRPCKVVCNTDVEVRLHQMDIHPDNREN 1154
Query: 1179 SETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALM 1238
+C+R HHWLH+K I+DVVE+L+GAFLV+ GFKAA +FL WIGI V+F + L
Sbjct: 1155 CNLRCTRSHHWLHRKVIADVVESLIGAFLVEGGFKAAFAFLHWIGIDVDFNNPALYRVLD 1214
Query: 1239 SSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCYQRLEFLGDAVLD 1298
SS+ + L D +I+ L+ +G++F HKGLLLQA VHPS+ +H GGCYQRLEFLGDAVL+
Sbjct: 1215 SSSINLSLMDYTDIAGLEELIGYKFKHKGLLLQAFVHPSFSQHSGGCYQRLEFLGDAVLE 1274
Query: 1299 YLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYV 1358
Y+ITSYLYS YP +KPG +TDLRS+ V N++ A AV++S +K+LI DS L S I +
Sbjct: 1275 YVITSYLYSTYPDIKPGQITDLRSLAVGNDSLAYAAVEKSIHKHLIKDSNHLTSAISKFE 1334
Query: 1359 NFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSG 1418
++K +S L P+CPKALGD+VESC+GAVL+D+GF++NYVWK+ML L P+++F+
Sbjct: 1335 MYVKLSNSEKDLLEEPACPKALGDIVESCIGAVLLDSGFNLNYVWKVMLMLLKPVLTFAN 1394
Query: 1419 FQLSPIRDIIEFCQDCGSNLQF-NESKTGKYYSVEAEVKGDNFHATASAAHRRKRDAKKI 1478
+P+R++ E CQ G L K Y V+ EV + +AA+R + A+K
Sbjct: 1395 MHTNPMRELRELCQCHGFELGLPKPMKADGEYHVKVEVNIKSKIIICTAANRNSKAARKF 1454
Query: 1479 AANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTIDQADNGFGTIKGLE 1538
AA L+KLK G SLEEIL +RK E +LIGY+E P ID + +K
Sbjct: 1455 AAQETLSKLKNYGYKHRNKSLEEILIVARKRESELIGYNEDP---IDVEADISVKMKSPH 1514
Query: 1539 SSGADLDPFVHSEVGNAHPARITRISKMLVSSSG--------------AAQEQLDPSLAF 1598
++ PF ++E R ++ +++ SG A Q PS A
Sbjct: 1515 IHEENI-PFQNTETSF---TRSSKFHNQIIAGSGKHDVNNGRNNQPKLATQSGRLPSEAT 1574
Query: 1599 D----AVSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEA 1654
+ V G + TARS L+E+CAAN+W PP F EEGPSHL+ FTYKVV+EI+ A
Sbjct: 1575 EKSNKKVYHGDMVHKTARSFLFELCAANYWKPPEFKLCKEEGPSHLRKFTYKVVVEIKGA 1634
BLAST of MS002366 vs. ExPASy Swiss-Prot
Match:
Q5N870 (Endoribonuclease Dicer homolog 3a OS=Oryza sativa subsp. japonica OX=39947 GN=DCL3A PE=2 SV=1)
HSP 1 Score: 712.6 bits (1838), Expect = 1.0e-203
Identity = 525/1681 (31.23%), Postives = 832/1681 (49.49%), Query Frame = 0
Query: 54 RKYQLELCKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKG-ICVFLAPTVALV 113
R+YQL++ + A+ N I L TG GKT IAV+L+ E + R G + +FLAPTV LV
Sbjct: 33 RRYQLDVYEVAMRRNTIAMLDTGAGKTMIAVMLIKEFGKINRTKNAGKVIIFLAPTVQLV 92
Query: 114 QQQAKVIEDSTDFKVRVYCGGSKCLN-SHYDWEREMEEHEVFVMTPEILLRNLHHCYIKM 173
QQ +VIE TDF+V Y G + W+ ++ +++V VMTP++ L+ L + ++ +
Sbjct: 93 TQQCEVIEIHTDFEVEQYYGAKGVDQWTGPRWQEQISKYQVMVMTPQVFLQALRNAFLIL 152
Query: 174 EFVELLIFDECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNS 233
+ V L+IFDECHHA +H Y IM+ FY + K P +FGMTASPV+ K
Sbjct: 153 DMVSLMIFDECHHA--TGNHPYTRIMKEFYHKSEHK-PSVFGMTASPVIRK--------- 212
Query: 234 GACNQQNLSKSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYG--PVA-NGLSGSF 293
G + + LE LLDAK+Y+V DREE++ V S YY PV LS
Sbjct: 213 GISSHLDCEGQFCELENLLDAKIYTVSDREEIEFCVPSAKEMCRYYDSKPVCFEDLSEEL 272
Query: 294 MTYCSLLEDMKRQCIVVLGQMKGEHEDL-RATKKLLCRMHDNIIFCLENLGVWGALQASK 353
CS + + + M + +D+ + +K+ L + I +CL+++G+ A +A+K
Sbjct: 273 GVLCSKYDALITELQNKRSDMYKDADDITKESKRRLSKSIAKICYCLDDVGLICASEATK 332
Query: 354 ILLSGDNSE--RSELIEAAEGNPN---------NHSLSDRYLNQAAEILAKGCKIGGGM- 413
I + + E+++A + + N +L ++ +A ++ K + G M
Sbjct: 333 ICIERGQEKGWLKEVVDATDQQTDANGSRLFAENSALHMKFFEEALHLIDKRLQQGIDML 392
Query: 414 --SDTLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCIIFVNRIVIARSLSYILQNLEF 473
S++ + + + S KL +LI+I SF + + +C+IFV+R + AR + +++ +
Sbjct: 393 LNSESGCVEAAKTGYISPKLYELIQIFHSFSNSRHARCLIFVDRKITARVIDRMIKKIGH 452
Query: 474 LAFWKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLLVATKVGEEGLDIQTCCLVIRF 533
LA + +L G S + +++ K L FRSG++NLL T V EEG+ + C VIRF
Sbjct: 453 LAHFTVSFLTGGRSSVDALTPKMQKDTLDSFRSGKVNLLFTTDVAEEGIHVPECSCVIRF 512
Query: 534 DLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLIDEFQKDENRMDREISSRSSSEI 593
DLP T S++QSRGRAR S+Y +++ GN K+ LI + E M + SSR S +
Sbjct: 513 DLPRTTRSYVQSRGRARQEDSQYILMIERGNVKQNDLISAIVRSETSMVKIASSRESGNL 572
Query: 594 ---FNSHEE-SIYRVASTGASITSGRSISLLHEFCSKLPHDEYFDPKPKFFYYDDLAGTV 653
F +EE + Y V +TGA +T+ SIS+++ +C KLP D+ + PKP F + G V
Sbjct: 573 SPGFVPNEEINEYHVGTTGAKVTADSSISIVYRYCEKLPQDKCYSPKPTFEFTHHDDGYV 632
Query: 654 CHAILPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGALNDFLLLMRDDANEEELE 713
C LP +A + ++ + AK+ C+ A ++LH+LGAL+D L L +D E
Sbjct: 633 CTLALPPSAVLQILVGPKARNMHKAKQLVCLDACKKLHELGALDDHLCLSVEDPVP---E 692
Query: 714 LDSSDSDRSEDETSRGELHEMLVPAALKDSWTGSGYFI-LNCYQIKFSPHPRDRIYKEFG 773
+ S + T R ELH A +W + L Y++ F +IY EF
Sbjct: 693 IVSKNKGTGIGTTKRKELHGTTRIHAWSGNWVSKKTALKLQSYKMNFVCDQAGQIYSEFV 752
Query: 774 LFVKAPLPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEITQAESFQEMFLKVILDR 833
L + A LP E ++I+L+L + V ++ G++EL ++ QA+ FQ + + +
Sbjct: 753 LLIDATLPDEVATLEIDLYL-HDKMVKTSVSSCGLLELDAQQMEQAKLFQGLLFNGLFGK 812
Query: 834 -------SEFVQEFIPLRNNVSKSISSTSYLLLPLIFHDNERSVSIDWKVIRRCLFSPIF 893
+EFI + + +++ YLLLP + +V I+W VI +
Sbjct: 813 LFTRSKVPNAPREFILNKEDTFVWNTASVYLLLP-TNPSFDSNVCINWSVIDAA--ATAV 872
Query: 894 QIPGSIVKETACSHAHLTLYDGRRRTSD-------------IKNSLVYVPYKREFFFVTD 953
++ I E L ++D + D +K+ +V + + + D
Sbjct: 873 KLMRRIYSEN--KRELLGIFDSDQNVGDLIHLANKSCKANSLKDMVVLAVHTGKIYTALD 932
Query: 954 I--VKGKNGYSRSHYKNSSSLSHF-EHLIERFGIHLKYPEQPLLSAKPLFHLHNLLHD-- 1013
I + G + + + K F E+ +++GI L++P QPLL KP + HNLL
Sbjct: 933 ITELSGDSAFDGASDKKECKFRTFAEYFKKKYGIVLRHPSQPLLVLKPSHNPHNLLSSKF 992
Query: 1014 RRQGN--------SEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLL 1073
R +GN + + +PPEL L + +I S L P++M+R+E+L
Sbjct: 993 RDEGNVVENMSNGTPVVNKTSNRVHMPPEL--LIPLDLPVEILRSFYLFPALMYRIESLT 1052
Query: 1074 VAIELKCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFL 1133
+A +L+ + + + +++ ILEA+TT +C E S+ERLE+LGD LK+AV+ +LFL
Sbjct: 1053 LASQLRSEIGYS---DSNISSFLILEAITTLRCSEDFSMERLELLGDSVLKYAVSCHLFL 1112
Query: 1134 THDKSDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLG----RPCPRI 1193
DEG+L+ R +++ N L+KL +N+Q Y+RD F+P ++ G RP P
Sbjct: 1113 KFPNKDEGQLSSIRCHMICNATLYKLGIERNVQGYVRDAAFDPRRWLAPGQLSIRPSPCE 1172
Query: 1194 C---NEETREDIHSHCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKA 1253
C +E +DIH A C +GH W+ KTI+D VEA++GA+ G +A
Sbjct: 1173 CPVKSEVVTDDIHI---IDDKAIVLGKACDKGHRWMCSKTIADCVEAIIGAYYAGGGLRA 1232
Query: 1254 AISFLKWIGIQVEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALV 1313
A++ LKW+GI E E L+ A++S++ L +L+ LG+ F KGLL++AL
Sbjct: 1233 AMAVLKWLGIGAEIEEDLIVQAILSASVQTYLPKDNVFEMLEAKLGYSFSVKGLLVEALT 1292
Query: 1314 HPSYHKHGGG-CYQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANV 1373
HPS + G CY+RLEFLGDAVLD L+T YL++++ G LTDLRS V NE FA V
Sbjct: 1293 HPSQQELGAKYCYERLEFLGDAVLDILLTRYLFNSHKDTNEGELTDLRSASVNNENFAQV 1352
Query: 1374 AVDRSFYKYLICDSTSLQSDIKSYVNFIKAPSSGSGSL---GLPSCPKALGDLVESCVGA 1433
AV +F+ +L S L I YVN ++ S L GLP PK LGD+VES GA
Sbjct: 1353 AVKHNFHHFLQHSSGLLLDQITEYVNRLEGSSMDKVELLSDGLPKGPKVLGDIVESIAGA 1412
Query: 1434 VLVDTGFDMNYVWKIMLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYS 1493
+L+DT D++ VW I L PI++ +L P R++IE+C G + N G
Sbjct: 1413 ILLDTKLDLDVVWGIFEPLLSPIVTPENLELPPYRELIEWCGKHGYFVGINCRDQGDTVV 1472
Query: 1494 VEAEVKGDNFHATASAAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEP 1553
+V+ ++++DAK A++L+L L+ +GLI I K++ K E
Sbjct: 1473 ATLDVQLKEVLLVRQGFSKKRKDAKAHASSLLLKDLEEKGLI--------IPKNASKTEQ 1532
Query: 1554 KLIGYDETPSVTIDQADNGFGTIKGLESSGADLDPFVHSEVGNAH-PARITRISKMLVSS 1613
E +PF N H A T+ K
Sbjct: 1533 -------------------------FEKHCGSTNPF-----NNLHVDAMDTQTPKPTKEK 1592
Query: 1614 SGAAQEQL-DPSLAFDAVSLGRLCKATARSRLYEICAANHWSPPSFD-----------CM 1650
+ A + DP L + + K R LYE+C W P+ + C
Sbjct: 1593 NAADSRNISDPLLGKPLHVIVKTSKGGPRIALYELCKKLQWPMPTMESEKVQPSFSSVCS 1646
BLAST of MS002366 vs. ExPASy Swiss-Prot
Match:
Q3EBC8 (Endoribonuclease Dicer homolog 2 OS=Arabidopsis thaliana OX=3702 GN=At3g03300 PE=1 SV=2)
HSP 1 Score: 661.8 bits (1706), Expect = 2.1e-188
Identity = 472/1435 (32.89%), Postives = 746/1435 (51.99%), Query Frame = 0
Query: 49 PRRKARKYQLELCKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPT 108
P AR YQ+E +KA+++N I++L TG GKT IA++L+ +L R+ CVFL P
Sbjct: 20 PLHFARSYQVEALEKAIKQNTIVFLETGSGKTLIAIMLLRSYAYLFRKPSPCFCVFLVPQ 79
Query: 109 VALVQQQAKVIEDSTDFKVRVYCG--GSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHH 168
V LV QQA+ ++ TD KV +Y G G +S W++E++++EV VMTP ILL L H
Sbjct: 80 VVLVTQQAEALKMHTDLKVGMYWGDMGVDFWDSS-TWKQEVDKYEVLVMTPAILLDALRH 139
Query: 169 CYIKMEFVELLIFDECHHAQVKSDHSYAEIMRVFY----KTNVAKRPRIFGMTASPVVGK 228
++ + +++LI DECHHA K H YA IMR FY + + PRIFGMTAS V K
Sbjct: 140 SFLSLSMIKVLIVDECHHAGGK--HPYACIMREFYHKELNSGTSNVPRIFGMTASLVKTK 199
Query: 229 DHCSTSTNSGACNQQNLSKSINSLEKLLDAKVYSVEDREELDAFV--SSPLVKIYYYGPV 288
N + K I+ LE L+++KVY+ E+ L FV S+P K Y + +
Sbjct: 200 GE----------NLDSYWKKIHELETLMNSKVYTCENESVLAGFVPFSTPSFKYYQHIKI 259
Query: 289 ANGLSGSFMTYCSLLEDMKRQCI---VVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENL 348
+ SL+E ++R I + LG + + + +K L R+ + +CL++L
Sbjct: 260 PS------PKRASLVEKLERLTIKHRLSLGTLDLNSSTVDSVEKRLLRISSTLTYCLDDL 319
Query: 349 GVWGALQASKILLSGDNSERSELIEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSD 408
G+ A +A++ L + N + E N + +L ++ + A++ G S
Sbjct: 320 GILLAQKAAQSLSASQN----DSFLWGELNMFSVALVKKFCSDASQEFLAEIPQGLNWSV 379
Query: 409 TLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCIIFVNRIVIARSL-SYILQNLEFLAF 468
E + K + LIE L + N++CIIFV+R++ A L S + + L
Sbjct: 380 ANINGNAEAGLLTLKTVCLIETLLGYSSLENIRCIIFVDRVITAIVLESLLAEILPNCNN 439
Query: 469 WKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLP 528
WK Y+ G +S L++ +RK N I+ FR G +N++VAT + EEGLD+Q+C LVIRFD
Sbjct: 440 WKTKYVAGNNSGLQNQTRKKQNEIVEDFRRGLVNIIVATSILEEGLDVQSCNLVIRFDPA 499
Query: 529 ETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLIDEFQKDENRMDREISSRS---SSEI 588
+ SFIQSRGRARM S+Y +V+SG+ + ++ RM E S +
Sbjct: 500 SNICSFIQSRGRARMQNSDYLMMVESGDLLTQSRLMKYLSGGKRMREESLDHSLVPCPPL 559
Query: 589 FNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAI 648
+ +E ++RV STGA++T S+SL++ +CS+LP DEYF P P+F D C
Sbjct: 560 PDDSDEPLFRVESTGATVTLSSSVSLIYHYCSRLPSDEYFKPAPRFDVNKDQGS--CTLY 619
Query: 649 LPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGALNDFLLLMRDDANEEELELDSS 708
LP + P+ ++ + + + + K+ C+KA +LHK+GAL+D L+ ++ + +
Sbjct: 620 LPKSCPVKEV--KAEANNKVLKQAVCLKACIQLHKVGALSDHLV--------PDMVVAET 679
Query: 709 DSDRSEDETSRGELHEMLVPAALKDSWTGSGYFILNCYQIKFSPH-PRDRIYKEFGLFVK 768
S + E E P L ++ + Y I+ P+ PR+ + L +
Sbjct: 680 VSQKLEKIQYNTE-QPCYFPPELVSQFSAQPETTYHFYLIRMKPNSPRNFHLNDVLLGTR 739
Query: 769 APLPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEITQAESFQEMFLKVILDRSEFV 828
L + L RG ++ V L G L ++E+ FQ +V+LD S V
Sbjct: 740 VVLEDDIGNTSFRLEDHRG-TIAVTLSYVGAFHLTQEEVLFCRRFQITLFRVLLDHS--V 799
Query: 829 QEFIPLRNNVS-KSISSTSYLLLPLIFHDNERSVSIDWKVIRRCLFSPIFQIPGSIVKET 888
+ + N + + + YLL+P H +E S+ IDW+VIR + + T
Sbjct: 800 ENLMEALNGLHLRDGVALDYLLVPST-HSHETSL-IDWEVIRSVNLTSHEVLEKHENCST 859
Query: 889 ACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKGKNGYSRSHYKNSSSLSHFE 948
+ L DG T ++N+LVY P+ + ++ NG S +NS ++ E
Sbjct: 860 NGASRILHTKDGLFCTCVVQNALVYTPHNGYVYCTKGVLNNLNGNSLLTKRNSGDQTYIE 919
Query: 949 HLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEACYLQEYFIDLPPELCQLKII 1008
+ ER GI L + ++PLL+ + +F LH+ LH ++ + F++LPPELC + +
Sbjct: 920 YYEERHGIQLNFVDEPLLNGRHIFTLHSYLHMAKKKKEKE--HDREFVELPPELCHVILS 979
Query: 1009 GFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGAEVTANRILEALTTEKCQER 1068
S D+ S + +PS+M R+E+LL+A LK + + ++LEA+TT+KC+++
Sbjct: 980 PISVDMIYSYTFIPSVMQRIESLLIAYNLKKSIPK-----VNIPTIKVLEAITTKKCEDQ 1039
Query: 1069 ISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYLVKNINLFKLATRKNLQVYI 1128
LE LE LGD FLK+AV + LF EG L+ ++ ++ N+ L + ++ LQ +I
Sbjct: 1040 FHLESLETLGDSFLKYAVCQQLFQHCHTHHEGLLSTKKDGMISNVMLCQFGCQQKLQGFI 1099
Query: 1129 RDQPFEPSQFYTLGRPCP--RICNEETREDIHSHCNATKNAKASETKCSRGHHWLHKKTI 1188
RD+ FEP + G+ + N+ E + + + +N K +K++
Sbjct: 1100 RDECFEPKGWMVPGQSSAAYSLVNDTLPESRNIYVASRRNLK--------------RKSV 1159
Query: 1189 SDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALMSSNAYIILADRINISVL 1248
+DVVE+L+GA+L + G AA+ F+ W+GI+V+F T + ++ I +N+ +
Sbjct: 1160 ADVVESLIGAYLSEGGELAALMFMNWVGIKVDF-----TTTKIQRDSPIQAEKLVNVGYM 1219
Query: 1249 QNSLGHQFLHKGLLLQALVHPSY-HKHGGGCYQRLEFLGDAVLDYLITSYLYSAYPKLKP 1308
++ L + F K LL++AL H SY CYQRLEFLGD+VLDYLIT +LY YP L P
Sbjct: 1220 ESLLNYSFEDKSLLVEALTHGSYMMPEIPRCYQRLEFLGDSVLDYLITKHLYDKYPCLSP 1279
Query: 1309 GLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYVNFIKAPS--SGSGSLG 1368
GLLTD+RS V NE +A VAV + +K+++ S L I V+ + S S G
Sbjct: 1280 GLLTDMRSASVNNECYALVAVKANLHKHILYASHHLHKHISRTVSEFEQSSLQSTFGWES 1339
Query: 1369 LPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSGFQLSPIRDIIEFCQ 1428
S PK LGD++ES GA+ VD+G++ V+ + L +I+ +L P+R++ E CQ
Sbjct: 1340 DISFPKVLGDVIESLAGAIFVDSGYNKEVVFASIKPLLGCMITPETVKLHPVRELTELCQ 1383
Query: 1429 DCGSNLQFNESKTGKYYSVEAEVKGDNFHATASAAHRRKRDAKKIAANLILTKLK 1462
QF SK + S EVK +A K+ AKK+A +L LK
Sbjct: 1400 ----KWQFELSKAKDFDSFTVEVKAKEMSFAHTAKASDKKMAKKLAYKEVLNLLK 1383
BLAST of MS002366 vs. ExPASy Swiss-Prot
Match:
Q7XD96 (Endoribonuclease Dicer homolog 3b OS=Oryza sativa subsp. japonica OX=39947 GN=DCL3B PE=3 SV=2)
HSP 1 Score: 639.0 bits (1647), Expect = 1.4e-181
Identity = 518/1651 (31.37%), Postives = 791/1651 (47.91%), Query Frame = 0
Query: 58 LELCKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQ--KGICVFLAPTVALVQQQ 117
+E+ + AL N I L TG GKT +AV+L E +R + + I VFLAPTV LV QQ
Sbjct: 44 VEVFEAALRGNTIAVLDTGSGKTMVAVMLAREHARRVRAGEAPRRIVVFLAPTVHLVHQQ 103
Query: 118 AKVIEDSTDFKVRVYCGGSKCLNSHYD-WEREMEEHEVFVMTPEILLRNLHHCYIKMEFV 177
+VI + TD V + G S+ D W+ E+ +E+ VMTP+ILL L H ++ M V
Sbjct: 104 FEVIREYTDLDVMMCSGASRVGEWGADHWKEEVGRNEIVVMTPQILLDALRHAFLTMSAV 163
Query: 178 ELLIFDECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGAC 237
LLIFDECH A H YA IM+ FY RP +FGMTASPV K GA
Sbjct: 164 SLLIFDECHRA--CGSHPYARIMKEFY-FGSQWRPDVFGMTASPVATK---------GAS 223
Query: 238 NQQNLSKSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSL 297
N I+ LE LDAK+Y VEDR EL++F + YY + + +
Sbjct: 224 TLHNCEAHISQLELTLDAKIYIVEDRNELESFSPPTTIVNKYYDAYMVDFD-NLKSKLQI 283
Query: 298 LEDMKRQCIVVL-----GQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKIL 357
D +V L + K L ++K L R H I++ L +LG + KI
Sbjct: 284 FSDEFDSLLVGLQESPSNKFKDTDNILETSRKSLSRYHGKILYSLNDLGPIITSEVVKIH 343
Query: 358 LSGDN--SERSELIEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGM-SDTLNADILEY 417
+ + + I + + S L+ EIL +G G M S++ + ++ +
Sbjct: 344 IESVKPLCDSEDCIFSKASLCLHMSYFKEALSLIEEILPQG--YGELMKSESGSEELTKR 403
Query: 418 PFFSKKLLQLIEILSSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVH 477
+ S K+ LI I SF + C+IFV+RI+ A+++ ++ + + + YL G
Sbjct: 404 GYISSKVNTLINIFKSFGSSNEVLCLIFVDRIITAKAVERFMRGIVNFSCFSISYLTGGS 463
Query: 478 SKLRSMSRKTMNHILMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSR 537
+ ++S L FR+G++NLL T V EEG+D+ C VIRFDLP TV S++QSR
Sbjct: 464 TSKDALSPAVQRFTLDLFRAGKVNLLFTTDVTEEGVDVPNCSCVIRFDLPRTVCSYVQSR 523
Query: 538 GRARMPQSEYAFLVDSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESI----- 597
GRAR SE+ +++ GN ++ I + + R + + S++ SI
Sbjct: 524 GRARRNNSEFILMIERGNLQQQEHIFRMIQTGYYVKNCALYRHPNAL--SYDLSIQGMYT 583
Query: 598 YRVASTGASITSGRSISLLHEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIH 657
Y+V STGA+IT+ ++L+ ++C KLP D YF PKP F + C LP NA
Sbjct: 584 YQVQSTGATITADCCVNLIRKYCEKLPKDRYFMPKPSFEVTIEDGLFKCTLTLPRNAAFQ 643
Query: 658 QIISRTQPSREGAKKDACIKAIEELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDE 717
I+ S +K+ ++A ++LH+LG LND L+ + ++ + +
Sbjct: 644 SIVGPLSSSSNLSKQLVSLEACKKLHQLGELNDHLVPLTEEPMDTDFTTADEKCISGPGT 703
Query: 718 TSRGELHEMLVPAALKDSWT-GSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAE 777
T R ELH AL +W S LN Y+I F Y F L ++ L +
Sbjct: 704 TKRKELHGTTCVLALSGTWIHDSENITLNTYRIDFLCDQEGENYAGFVLLMEPELDDDVA 763
Query: 778 RMDIELHLARGRSVMVNLVPSGVVELLEDEITQAESFQEMFLKVILDR-------SEFVQ 837
++L L + V + P G V+L + ++ + + FQE F I R S +
Sbjct: 764 PSKMDLFLIPNKMVYTTVTPRGKVQLNKKQLGKGKLFQEFFFNGIFGRLFHGSRKSGAQR 823
Query: 838 EFIPLRNNVSKSISSTSYLLLPLIFHDN---ERSVSIDWKVIRRC------LFS------ 897
+FI + + + + + YLLLPL D+ + +SI W+ I C L+S
Sbjct: 824 DFIFKKGHEIQWNTESMYLLLPL--RDSSYIQDDLSIHWEAIESCAGAVEQLWSSYQGDE 883
Query: 898 ---PIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIV---KG 957
P+ IP K + L + S IK+S+V + + V D++
Sbjct: 884 NVIPVNCIP---QKRRGGQEEIIHLANKSLHCSSIKDSVVLSLHTGRIYTVLDLILDTTA 943
Query: 958 KNGYSRSHYKNSSSLSHF-EHLIERFGIHLKYPEQPLLSAKPLFHLHNLLH------DRR 1017
++ + +S + F ++ +++GI +++PEQPLL K + HNLL D
Sbjct: 944 EDSFDEMCKGKASPFTSFVDYYHQKYGIIIQHPEQPLLLLKQSHNAHNLLFSKLKYLDGS 1003
Query: 1018 QGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLA 1077
G ++ +PPEL L + + DI S LLPS++HRL++L++A +L+ +
Sbjct: 1004 TGKPLLMEKEQIHARVPPEL--LIHLDVTTDILKSFYLLPSVIHRLQSLMLASQLRREIG 1063
Query: 1078 AAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKSDEGEL 1137
+ ILEA+TT +C E SLERLE+LGD LK+ V LFL + EG+L
Sbjct: 1064 Y----NQHIPVTLILEAITTLRCCETFSLERLELLGDSVLKYVVGCDLFLRYPMKHEGQL 1123
Query: 1138 TRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLG----RPCPRICNEETREDIH 1197
+ RS V N L K ++LQ Y+RD F+P ++ G RP P C ET +
Sbjct: 1124 SDMRSKAVCNATLHKHGIWRSLQGYVRDNAFDPRRWVAPGQISLRPFPCNCGIET-AFVP 1183
Query: 1198 SHCNATKNAKA--SETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQ 1257
SH ++ + C RGH W+ KTISD VEALVGA+ V G AA+ ++W GI
Sbjct: 1184 SHRRYIRDDPSFFVGKPCDRGHRWMCSKTISDCVEALVGAYYVGGGIAAALWVMRWFGID 1243
Query: 1258 VEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHG-GG 1317
++ + L+ +++ L+ +I L+ L + F KGLLL+A+ HPS + G
Sbjct: 1244 IKCDMKLLQEVKFNASHLCSLSKINDIEELEAKLKYNFSVKGLLLEAITHPSLQELGVDY 1303
Query: 1318 CYQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLI 1377
CYQRLEFLGD+VLD L+T +LY+ + + PG LTDLRS V NE FA V + + +L
Sbjct: 1304 CYQRLEFLGDSVLDLLLTRHLYATHTDVDPGELTDLRSALVSNENFAQAVVRNNIHSHLQ 1363
Query: 1378 CDSTSLQSDIKSYV-NFIKAPSSGSGSLGLPSC--PKALGDLVESCVGAVLVDTGFDMNY 1437
S L I YV + ++ S L +C PK LGD++ES GAV +DT F+++
Sbjct: 1364 HGSGILLEQITEYVRSNLECQGKESEFLQHTTCKVPKVLGDIMESIAGAVFIDTDFNVDM 1423
Query: 1438 VWKIMLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFH 1497
VW+I L P+I+ L P R+++E C G L + G+ +E ++ +
Sbjct: 1424 VWEIFEPLLSPLITPDKLALPPYRELLELCSHIGCFLNSKCTSKGEEVIIEMSLQLRDEL 1483
Query: 1498 ATASAAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSV 1557
A R K+ AK AA+ IL LK QGL S+++ L +++++
Sbjct: 1484 LVAQGHDRNKKRAKAKAASRILADLKQQGL-----SIKQCLSKAKQLD------------ 1543
Query: 1558 TIDQADNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSL 1617
I +D F L SSG L +S++ + H +L S +E + P
Sbjct: 1544 -IVTSDLQF----DLTSSGTQLS---YSDLNDYH---------ILEGLSSVKKEVVLPL- 1603
Query: 1618 AFDAVSLGRLCKATARSRLYEICAANHWSPPSFD----------CMNEEGPSHLKMFTYK 1637
++ K RS L+++C W P F+ M+ ++ F
Sbjct: 1604 --------KMEKGGPRSALFKLCKILQWPMPEFEFVEQRFRTPIVMDGATTTNFNSFVST 1620
BLAST of MS002366 vs. ExPASy TrEMBL
Match:
A0A6J1C050 (dicer-like protein 4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007071 PE=3 SV=1)
HSP 1 Score: 3233.0 bits (8381), Expect = 0.0e+00
Identity = 1631/1661 (98.19%), Postives = 1636/1661 (98.49%), Query Frame = 0
Query: 1 MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPSTRASEKDPRRKARKYQLEL 60
MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPS+RASEKDPRRKARKYQLEL
Sbjct: 2 MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPSSRASEKDPRRKARKYQLEL 61
Query: 61 CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE 120
CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE
Sbjct: 62 CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE 121
Query: 121 DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFD 180
DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLR LHHCYIKMEFVELLIFD
Sbjct: 122 DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRTLHHCYIKMEFVELLIFD 181
Query: 181 ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLS 240
ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGK GACNQQNLS
Sbjct: 182 ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGK---------GACNQQNLS 241
Query: 241 KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKR 300
KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKR
Sbjct: 242 KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKR 301
Query: 301 QCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSEL 360
QCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSEL
Sbjct: 302 QCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSEL 361
Query: 361 IEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEIL 420
IEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEIL
Sbjct: 362 IEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEIL 421
Query: 421 SSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHI 480
SSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHI
Sbjct: 422 SSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHI 481
Query: 481 LMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540
LMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV
Sbjct: 482 LMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 541
Query: 541 DSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLL 600
DSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLL
Sbjct: 542 DSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLL 601
Query: 601 HEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACI 660
HEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACI
Sbjct: 602 HEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACI 661
Query: 661 KAIEELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSW 720
KAIEELHKLGALNDFLL MRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSW
Sbjct: 662 KAIEELHKLGALNDFLLPMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSW 721
Query: 721 TGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVP 780
TGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKA LPQEAERMDIELHLARGRSVMVNLVP
Sbjct: 722 TGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAALPQEAERMDIELHLARGRSVMVNLVP 781
Query: 781 SGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHD 840
SGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHD
Sbjct: 782 SGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHD 841
Query: 841 NERSVSIDWKVIRRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYK 900
NERSVSIDWKVIRRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYK
Sbjct: 842 NERSVSIDWKVIRRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYK 901
Query: 901 REFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNL 960
REFFFVTDIVKGKNGYSRSHYKNSSS SHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNL
Sbjct: 902 REFFFVTDIVKGKNGYSRSHYKNSSSSSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNL 961
Query: 961 LHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIEL 1020
LHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIEL
Sbjct: 962 LHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIEL 1021
Query: 1021 KCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKS 1080
KCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKS
Sbjct: 1022 KCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKS 1081
Query: 1081 DEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDI 1140
DEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDI
Sbjct: 1082 DEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDI 1141
Query: 1141 HSHCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQV 1200
HSHCNATKNA+ASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQV
Sbjct: 1142 HSHCNATKNAQASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQV 1201
Query: 1201 EFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCY 1260
EFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCY
Sbjct: 1202 EFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCY 1261
Query: 1261 QRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICD 1320
QRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICD
Sbjct: 1262 QRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICD 1321
Query: 1321 STSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIM 1380
STSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIM
Sbjct: 1322 STSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIM 1381
Query: 1381 LSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHATASA 1440
LSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEA+VKGDNFHATASA
Sbjct: 1382 LSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEADVKGDNFHATASA 1441
Query: 1441 AHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTIDQA 1500
AHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVT+DQA
Sbjct: 1442 AHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTMDQA 1501
Query: 1501 DNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFDA- 1560
DNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFD
Sbjct: 1502 DNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFDGH 1561
Query: 1561 -------VSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEE 1620
S GRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEE
Sbjct: 1562 DSPTDVQSSSGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEE 1621
Query: 1621 APNTIFKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYLPETD 1654
AP+TIFKFFGAPHLRKKAAAEHAAEAAIWYLKK GYLPETD
Sbjct: 1622 APDTIFKFFGAPHLRKKAAAEHAAEAAIWYLKKEGYLPETD 1653
BLAST of MS002366 vs. ExPASy TrEMBL
Match:
A0A6J1BZQ8 (dicer-like protein 4 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007071 PE=4 SV=1)
HSP 1 Score: 2755.3 bits (7141), Expect = 0.0e+00
Identity = 1389/1409 (98.58%), Postives = 1394/1409 (98.94%), Query Frame = 0
Query: 253 KVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKRQCIVVLGQMKGE 312
+VYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKRQCIVVLGQMKGE
Sbjct: 10 EVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKRQCIVVLGQMKGE 69
Query: 313 HEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNHS 372
HEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNHS
Sbjct: 70 HEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAEGNPNNHS 129
Query: 373 LSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCI 432
LSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCI
Sbjct: 130 LSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCI 189
Query: 433 IFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLL 492
IFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLL
Sbjct: 190 IFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLL 249
Query: 493 VATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLID 552
VATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLID
Sbjct: 250 VATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLID 309
Query: 553 EFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEY 612
EFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEY
Sbjct: 310 EFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEY 369
Query: 613 FDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGAL 672
FDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGAL
Sbjct: 370 FDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGAL 429
Query: 673 NDFLLLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSWTGSGYFILNCYQ 732
NDFLL MRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSWTGSGYFILNCYQ
Sbjct: 430 NDFLLPMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSWTGSGYFILNCYQ 489
Query: 733 IKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEIT 792
IKFSPHPRDRIYKEFGLFVKA LPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEIT
Sbjct: 490 IKFSPHPRDRIYKEFGLFVKAALPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEIT 549
Query: 793 QAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHDNERSVSIDWKVI 852
QAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHDNERSVSIDWKVI
Sbjct: 550 QAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHDNERSVSIDWKVI 609
Query: 853 RRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKG 912
RRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKG
Sbjct: 610 RRCLFSPIFQIPGSIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKG 669
Query: 913 KNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEAC 972
KNGYSRSHYKNSSS SHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEAC
Sbjct: 670 KNGYSRSHYKNSSSSSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEAC 729
Query: 973 YLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGA 1032
YLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGA
Sbjct: 730 YLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGA 789
Query: 1033 EVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYL 1092
EVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYL
Sbjct: 790 EVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYL 849
Query: 1093 VKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDIHSHCNATKNAKA 1152
VKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDIHSHCNATKNA+A
Sbjct: 850 VKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETREDIHSHCNATKNAQA 909
Query: 1153 SETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALM 1212
SETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALM
Sbjct: 910 SETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALM 969
Query: 1213 SSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCYQRLEFLGDAVLD 1272
SSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCYQRLEFLGDAVLD
Sbjct: 970 SSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGCYQRLEFLGDAVLD 1029
Query: 1273 YLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYV 1332
YLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYV
Sbjct: 1030 YLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYV 1089
Query: 1333 NFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSG 1392
NFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSG
Sbjct: 1090 NFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSG 1149
Query: 1393 FQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHATASAAHRRKRDAKKIA 1452
FQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEA+VKGDNFHATASAAHRRKRDAKKIA
Sbjct: 1150 FQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEADVKGDNFHATASAAHRRKRDAKKIA 1209
Query: 1453 ANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTIDQADNGFGTIKGLES 1512
ANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVT+DQADNGFGTIKGLES
Sbjct: 1210 ANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTMDQADNGFGTIKGLES 1269
Query: 1513 SGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFDA--------VSLGR 1572
SGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFD S GR
Sbjct: 1270 SGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFDGHDSPTDVQSSSGR 1329
Query: 1573 LCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEAPNTIFKFFGAP 1632
LCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEAP+TIFKFFGAP
Sbjct: 1330 LCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEAPDTIFKFFGAP 1389
Query: 1633 HLRKKAAAEHAAEAAIWYLKKAGYLPETD 1654
HLRKKAAAEHAAEAAIWYLKK GYLPETD
Sbjct: 1390 HLRKKAAAEHAAEAAIWYLKKEGYLPETD 1418
BLAST of MS002366 vs. ExPASy TrEMBL
Match:
A0A6J1JAJ5 (dicer-like protein 4 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485046 PE=3 SV=1)
HSP 1 Score: 2680.2 bits (6946), Expect = 0.0e+00
Identity = 1376/1676 (82.10%), Postives = 1468/1676 (87.59%), Query Frame = 0
Query: 1 MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPSTRASEKDPRRKARKYQLEL 60
MPDG+TNS ADS NFQ T VE F+GLS VDDQ G S R S KDPRR ARKYQLEL
Sbjct: 1 MPDGETNSTTADSVNFQPTISASVERFMGLSPVDDQGEGSSIRTSGKDPRRIARKYQLEL 60
Query: 61 CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE 120
CKKALEENII+YLGTGCGKTHIA+LL+YEM HLIRR QKGICVFLAPTVALVQQQAKVIE
Sbjct: 61 CKKALEENIIVYLGTGCGKTHIAILLIYEMGHLIRRPQKGICVFLAPTVALVQQQAKVIE 120
Query: 121 DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFD 180
DS DFKV+VYCGGSKCLNSHYDWEREMEE+EVFVMTPEILLRNLHHCYIKMEFVELLIFD
Sbjct: 121 DSIDFKVKVYCGGSKCLNSHYDWEREMEEYEVFVMTPEILLRNLHHCYIKMEFVELLIFD 180
Query: 181 ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLS 240
ECHHAQVKSDHSYAEIMRVFYK N KRPRIFGMTASPVVGK GACNQQNLS
Sbjct: 181 ECHHAQVKSDHSYAEIMRVFYKANEHKRPRIFGMTASPVVGK---------GACNQQNLS 240
Query: 241 KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKR 300
KSINSLEKLLDAKVYSVEDREEL+AFVSSP KIYYYGPVA G S SFMTYC LED+K
Sbjct: 241 KSINSLEKLLDAKVYSVEDREELEAFVSSPRFKIYYYGPVATGSSSSFMTYCCPLEDVKC 300
Query: 301 QCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSEL 360
QCIV LGQMKGEHE L ATKKLLCRMH NIIFCLENLGVWGALQASKILLS DNSERSEL
Sbjct: 301 QCIVALGQMKGEHEGLLATKKLLCRMHKNIIFCLENLGVWGALQASKILLSEDNSERSEL 360
Query: 361 IEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEIL 420
IEAAEGNP S+SDRYLNQAAEI A GCKI G+SD LN DIL+ P FSKKLL+LI IL
Sbjct: 361 IEAAEGNPKYDSISDRYLNQAAEIFASGCKIDAGVSDMLNVDILKEPCFSKKLLRLIGIL 420
Query: 421 SSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHI 480
SSFRHR NMKCIIFVNRIVIARSLSYILQNL+FLA+WKCD+LVGVHSKLRSMSRKTMNHI
Sbjct: 421 SSFRHRPNMKCIIFVNRIVIARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNHI 480
Query: 481 LMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540
L KFRSGELNLL+ATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV
Sbjct: 481 LTKFRSGELNLLIATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540
Query: 541 DSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLL 600
DSGNEK+LGLIDEF KDE RM+REISSRSS E F+ HEESIYRV STGAS+TSGRSISLL
Sbjct: 541 DSGNEKDLGLIDEFHKDEKRMNREISSRSSIETFDGHEESIYRVVSTGASVTSGRSISLL 600
Query: 601 HEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACI 660
HE+CSKLPHD YFDPKPKFFY DDL GT+CHAILPSNAPI QI+SR QPS AKKDAC+
Sbjct: 601 HEYCSKLPHDTYFDPKPKFFYCDDLGGTICHAILPSNAPIPQIVSRPQPSGVAAKKDACL 660
Query: 661 KAIEELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSW 720
KA+EELHKLGAL+D+LL MRD+ANE ELELD SDSD SEDETSR ELHEM+ PAA K+SW
Sbjct: 661 KAVEELHKLGALSDYLLPMRDNANEGELELD-SDSDSSEDETSRLELHEMISPAAFKESW 720
Query: 721 TGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVP 780
TG GY IL CY IKF+P+P DRIYKEFGLFVKAPLPQEA RMD+ELHLA GRSV VNL+P
Sbjct: 721 TGFGYVILYCYHIKFTPNPEDRIYKEFGLFVKAPLPQEAVRMDLELHLAHGRSVKVNLMP 780
Query: 781 SGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHD 840
S VVELLE+EITQAE FQEMFLKVILDRSEFV ++IPLRNNVS+SISSTSYLLLP+I HD
Sbjct: 781 SRVVELLENEITQAECFQEMFLKVILDRSEFVHDYIPLRNNVSRSISSTSYLLLPVIVHD 840
Query: 841 NERSVSIDWKVIRRCLFSPIFQIPG-SIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPY 900
NE SV IDWKVIRRCL S IFQ SI+KETA S HL LYDGRRR+SDI+NSLVYVPY
Sbjct: 841 NEGSVFIDWKVIRRCLSSQIFQNHACSIIKETASSDTHLMLYDGRRRSSDIENSLVYVPY 900
Query: 901 KREFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHN 960
KREFFFVT+IV+ KNGYS+ YK+S SLSHFEHL RFGIHLKYPEQPLL AKPLF LHN
Sbjct: 901 KREFFFVTNIVRRKNGYSQ--YKDSGSLSHFEHLKTRFGIHLKYPEQPLLCAKPLFSLHN 960
Query: 961 LLHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIE 1020
LLH+R+Q +SEA YL+EYFID+ PELCQLKI GFSKDIGSSISLLPSIMHRLENLLVAIE
Sbjct: 961 LLHNRKQKDSEAPYLEEYFIDVAPELCQLKITGFSKDIGSSISLLPSIMHRLENLLVAIE 1020
Query: 1021 LKCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDK 1080
LKCMLAAAFP GAEVTANRILEALTTEKCQER SLERLEILGD FLKFAVARYLFL HDK
Sbjct: 1021 LKCMLAAAFPAGAEVTANRILEALTTEKCQERFSLERLEILGDSFLKFAVARYLFLAHDK 1080
Query: 1081 SDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETRED 1140
DEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFY LGRPCPRICNEETR D
Sbjct: 1081 FDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYLLGRPCPRICNEETRND 1140
Query: 1141 IHSHCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQ 1200
IHSH NAT NAK SETKCS+GHHWL KKTISD+VEAL+GAFLVDSGFKAAI+FLKWIGI+
Sbjct: 1141 IHSHDNATNNAKFSETKCSKGHHWLQKKTISDMVEALIGAFLVDSGFKAAIAFLKWIGIK 1200
Query: 1201 VEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGC 1260
VEFEAS VTNAL++S AY++ AD INIS LQ+SLGH+FLHKGLLLQALVHP+YHKHGGGC
Sbjct: 1201 VEFEASQVTNALVASKAYVLFADTINISALQSSLGHKFLHKGLLLQALVHPTYHKHGGGC 1260
Query: 1261 YQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLIC 1320
YQRLEFLGDAVLDYLI SYLYS YPKLKPGLLTDLRSVFVRNEAFANVAVDR FYK+L+C
Sbjct: 1261 YQRLEFLGDAVLDYLIMSYLYSVYPKLKPGLLTDLRSVFVRNEAFANVAVDRFFYKFLLC 1320
Query: 1321 DSTSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKI 1380
DS SL+SDIKSYV+FIKAP S SL P CPK LGDLVES VGAVLVDTGFDMNYVWKI
Sbjct: 1321 DSASLESDIKSYVDFIKAPPSERDSLEQPRCPKPLGDLVESSVGAVLVDTGFDMNYVWKI 1380
Query: 1381 MLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHATAS 1440
MLSFLDPI+S SGFQLSPIRDIIE CQ+CG LQFN SK KYYSVEAEVKGD+FHATAS
Sbjct: 1381 MLSFLDPIMSSSGFQLSPIRDIIEVCQNCGWKLQFNPSKMEKYYSVEAEVKGDDFHATAS 1440
Query: 1441 AAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTIDQ 1500
A +RRK+DA+KIAAN IL KLKAQG IPEVNSLE+ILKSS KM+PKLIGYDETPSVT+D+
Sbjct: 1441 AVNRRKKDAEKIAANAILRKLKAQGFIPEVNSLEKILKSSEKMKPKLIGYDETPSVTMDR 1500
Query: 1501 ADNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFD- 1560
D+ GLE SGADLDP VH EVGN+ P RITR+S+ L SSSGAA EQL PS+ +
Sbjct: 1501 IDD------GLEFSGADLDPLVHREVGNSRPFRITRMSETLASSSGAAGEQLKPSIPLEG 1560
Query: 1561 ---------------------AVSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPS 1620
+ S GR K TARSRLYE+CA NHW+ PSF+CMNEEGPS
Sbjct: 1561 HDSSTDLLSSSSSSSSSSSSSSSSSGRSGKTTARSRLYEVCAINHWNHPSFNCMNEEGPS 1620
Query: 1621 HLKMFTYKVVLEIEEAPNTIFKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYLPETD 1654
HLKMFTYKVVLEIEEAP+TIF+FFGAPHL KKAAAEHAAEAA+WYLKK YL +TD
Sbjct: 1621 HLKMFTYKVVLEIEEAPDTIFEFFGAPHLTKKAAAEHAAEAALWYLKKERYLGDTD 1658
BLAST of MS002366 vs. ExPASy TrEMBL
Match:
A0A6J1E1P4 (dicer-like protein 4 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429985 PE=3 SV=1)
HSP 1 Score: 2676.7 bits (6937), Expect = 0.0e+00
Identity = 1377/1672 (82.36%), Postives = 1469/1672 (87.86%), Query Frame = 0
Query: 1 MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPSTRASEKDPRRKARKYQLEL 60
MPDG+ NS ADS NFQ TS VE F+GLS VDDQ G S R S KDPRR ARKYQLEL
Sbjct: 1 MPDGEINSTTADSVNFQPTSSASVERFMGLSPVDDQGAGSSIRTSGKDPRRIARKYQLEL 60
Query: 61 CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE 120
C+KALEENII+YLGTGCGKTHIA+LL+YEM HLIRR QKGICVFLAPTVALVQQQAKVIE
Sbjct: 61 CEKALEENIIVYLGTGCGKTHIAILLIYEMGHLIRRPQKGICVFLAPTVALVQQQAKVIE 120
Query: 121 DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFD 180
DS DFKV+VYCGGSKCLNSHYDWEREMEE+EVFVMTPEILLRNLHHCYI+MEFVELLIFD
Sbjct: 121 DSIDFKVKVYCGGSKCLNSHYDWEREMEEYEVFVMTPEILLRNLHHCYIRMEFVELLIFD 180
Query: 181 ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLS 240
ECHHAQVKSDHSYAEIMRVFYK N KRPRIFGMTASPVVGK GACNQQNLS
Sbjct: 181 ECHHAQVKSDHSYAEIMRVFYKANEHKRPRIFGMTASPVVGK---------GACNQQNLS 240
Query: 241 KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKR 300
KSINSLEKLLDAKVYSVEDREEL+AFVSSP KIYYYGPVA G S SFMTYC LED+K
Sbjct: 241 KSINSLEKLLDAKVYSVEDREELEAFVSSPRFKIYYYGPVATGSSSSFMTYCCPLEDVKC 300
Query: 301 QCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSEL 360
QCIV LGQMKGEHE L ATKKLLCRMH NIIFCLENLGVWGALQASKILLS DNSERSEL
Sbjct: 301 QCIVALGQMKGEHEGLLATKKLLCRMHKNIIFCLENLGVWGALQASKILLSEDNSERSEL 360
Query: 361 IEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEIL 420
IEAA NP + S+SDRYLNQAAEI A GCKI G+SD LN DIL+ P FSKKLL+LI IL
Sbjct: 361 IEAAAANPKHDSISDRYLNQAAEIFASGCKIDAGVSDMLNVDILKEPCFSKKLLRLIGIL 420
Query: 421 SSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHI 480
SSFRHR NMKCIIFVNRIVIARSLSYILQNL+FLA+WKCD+LVGVHSKLRSMSRKTMNHI
Sbjct: 421 SSFRHRPNMKCIIFVNRIVIARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNHI 480
Query: 481 LMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540
L KFRSGELNLL+ATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV
Sbjct: 481 LTKFRSGELNLLIATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540
Query: 541 DSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLL 600
DSGNEKELGLIDEF KDE RM+REISSRSS E F+ HEESIYRV STGAS+TSGRSISLL
Sbjct: 541 DSGNEKELGLIDEFHKDEKRMNREISSRSSVETFDGHEESIYRVISTGASVTSGRSISLL 600
Query: 601 HEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACI 660
HE+CSKLPHD YFDPKPKFFY DDL GT+CHAILPSNAPI QI+SR QPS AKKDAC+
Sbjct: 601 HEYCSKLPHDTYFDPKPKFFYCDDLGGTICHAILPSNAPIPQIVSRPQPSGVAAKKDACL 660
Query: 661 KAIEELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSW 720
KA+EELHKLGAL+D+LL MRD+ANE ELELD SDSD SEDETSR ELHEM+ PAA K+SW
Sbjct: 661 KAVEELHKLGALSDYLLPMRDNANEGELELD-SDSDSSEDETSRRELHEMISPAAFKESW 720
Query: 721 TGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVP 780
TG GY IL CY IKF+P+P DRIYKEFGLFVKAPLPQEA RMD+ELHLA GRSV VNL+P
Sbjct: 721 TGFGYVILYCYHIKFTPNPEDRIYKEFGLFVKAPLPQEAVRMDLELHLAHGRSVKVNLMP 780
Query: 781 SGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHD 840
S VVEL+E+EITQAE FQEMFLKVILDRSEFV ++IPLRNNVS+SISSTSYLLLP+I HD
Sbjct: 781 SRVVELMENEITQAECFQEMFLKVILDRSEFVHDYIPLRNNVSRSISSTSYLLLPVIVHD 840
Query: 841 NERSVSIDWKVIRRCLFSPIFQIPG-SIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPY 900
E SV IDWKVIRRCL S IFQ SIVKETA S HL LYDGRRR+SDI+NSLVYVPY
Sbjct: 841 IEGSVFIDWKVIRRCLSSQIFQNHACSIVKETASSDTHLILYDGRRRSSDIENSLVYVPY 900
Query: 901 KREFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHN 960
KREFFFVT+IV+ KNGYS+ YK+S SLSHFEHL RFGI LKYPEQPLL AKPLF LHN
Sbjct: 901 KREFFFVTNIVRRKNGYSQ--YKDSGSLSHFEHLKTRFGIQLKYPEQPLLCAKPLFSLHN 960
Query: 961 LLHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIE 1020
LLHDR+Q +SEA YL+EYFID+ PELCQLKI GFSKDIGSSISLLPSIMHRLENLLVAIE
Sbjct: 961 LLHDRKQKDSEAPYLEEYFIDIAPELCQLKITGFSKDIGSSISLLPSIMHRLENLLVAIE 1020
Query: 1021 LKCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDK 1080
LKCMLAA FP GAEVTANRILEALTTEKCQERISLERLEILGD FLKFAVARYLFL HDK
Sbjct: 1021 LKCMLAAGFPAGAEVTANRILEALTTEKCQERISLERLEILGDSFLKFAVARYLFLAHDK 1080
Query: 1081 SDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETRED 1140
DEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFY LGRPCPRICNEETR D
Sbjct: 1081 FDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYLLGRPCPRICNEETRND 1140
Query: 1141 IHSHCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQ 1200
IHSH NAT NAKASETKCS+GHHWL KKTISDVVEALVGAFLVDSGFKAAI+FLKWIGI+
Sbjct: 1141 IHSHDNATNNAKASETKCSKGHHWLQKKTISDVVEALVGAFLVDSGFKAAIAFLKWIGIK 1200
Query: 1201 VEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGC 1260
VEFEAS VTNAL++S AY++LAD INIS LQ+SLG++FLHKGLLLQALVHP+YHKHGGGC
Sbjct: 1201 VEFEASQVTNALVASKAYVLLADTINISALQSSLGYKFLHKGLLLQALVHPTYHKHGGGC 1260
Query: 1261 YQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLIC 1320
YQRLEFLGDAVLDYLI SYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDR FYK+L+C
Sbjct: 1261 YQRLEFLGDAVLDYLIMSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRFFYKFLLC 1320
Query: 1321 DSTSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKI 1380
DS SLQSDIKSYV+FIK+ S SL P CPK LGDLVES VGAVLVDTGFDMNYVWKI
Sbjct: 1321 DSASLQSDIKSYVDFIKSLPSERDSLEQPRCPKPLGDLVESSVGAVLVDTGFDMNYVWKI 1380
Query: 1381 MLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHATAS 1440
MLSFLDPI+S SGFQLSPIRDIIE CQ+CG LQFN SK KYYSVEAEVKGD+FHATAS
Sbjct: 1381 MLSFLDPIMSSSGFQLSPIRDIIEVCQNCGWKLQFNPSKMEKYYSVEAEVKGDDFHATAS 1440
Query: 1441 AAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTIDQ 1500
A +RRK+DA+KIAAN ILTKLKAQG IPEVNSLE+ILKSS KM+PKLIGYDETPSVT+DQ
Sbjct: 1441 AVNRRKKDAEKIAANSILTKLKAQGFIPEVNSLEKILKSSEKMKPKLIGYDETPSVTMDQ 1500
Query: 1501 ADNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFD- 1560
D+ GLE SGADLDP VH EVGN+HP RITR+S+ L SSSGAA EQL PS+ +
Sbjct: 1501 IDD------GLEFSGADLDPRVHHEVGNSHPFRITRMSETLASSSGAAGEQLKPSIPLEG 1560
Query: 1561 -----------------AVSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKM 1620
+ S GR K TARSRL+E+CA NHW+ PSF+CMNEEGPSHLKM
Sbjct: 1561 HDSSTDLLSSSSSSSSSSSSSGRSGKTTARSRLHEVCAINHWNHPSFNCMNEEGPSHLKM 1620
Query: 1621 FTYKVVLEIEEAPNTIFKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYLPETD 1654
FTYKVVLEIEEAP+TIF+FFGAPHL KKAAAEHAAEAA+WYLKK YL +TD
Sbjct: 1621 FTYKVVLEIEEAPDTIFEFFGAPHLTKKAAAEHAAEAALWYLKKERYLGDTD 1654
BLAST of MS002366 vs. ExPASy TrEMBL
Match:
A0A6J1JJM5 (dicer-like protein 4 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485046 PE=3 SV=1)
HSP 1 Score: 2673.7 bits (6929), Expect = 0.0e+00
Identity = 1375/1676 (82.04%), Postives = 1467/1676 (87.53%), Query Frame = 0
Query: 1 MPDGDTNSMAADSDNFQHTSLGPVESFVGLSLVDDQEPGPSTRASEKDPRRKARKYQLEL 60
MPDG+TNS ADS NFQ T VE F+GLS VDDQ G S R S KDPRR ARKYQLEL
Sbjct: 1 MPDGETNSTTADSVNFQPTISASVERFMGLSPVDDQGEGSSIRTSGKDPRRIARKYQLEL 60
Query: 61 CKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIE 120
CKKALEENII+YLGTGCGKTHIA+LL+YEM HLIRR QKGICVFLAPTVALVQQQAKVIE
Sbjct: 61 CKKALEENIIVYLGTGCGKTHIAILLIYEMGHLIRRPQKGICVFLAPTVALVQQQAKVIE 120
Query: 121 DSTDFKVRVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFD 180
DS DFKV+VYCGGSKCLNSHYDWEREMEE+EVFVMTPEILLRNLHHCYIKMEFVELLIFD
Sbjct: 121 DSIDFKVKVYCGGSKCLNSHYDWEREMEEYEVFVMTPEILLRNLHHCYIKMEFVELLIFD 180
Query: 181 ECHHAQVKSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLS 240
ECHHAQVKSDHSYAEIMRVFYK N KRPRIFGMTASPVVGK GACNQQNLS
Sbjct: 181 ECHHAQVKSDHSYAEIMRVFYKANEHKRPRIFGMTASPVVGK---------GACNQQNLS 240
Query: 241 KSINSLEKLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKR 300
KSINSLEKLLDAKVYSVEDREEL+AFVSSP KIYYYGPVA G S SFMTYC LED+K
Sbjct: 241 KSINSLEKLLDAKVYSVEDREELEAFVSSPRFKIYYYGPVATGSSSSFMTYCCPLEDVKC 300
Query: 301 QCIVVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSEL 360
QCIV LGQMKGEHE L ATKKLLCRMH NIIFCLENLGVWGALQASKILLS DNSERSEL
Sbjct: 301 QCIVALGQMKGEHEGLLATKKLLCRMHKNIIFCLENLGVWGALQASKILLSEDNSERSEL 360
Query: 361 IEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEIL 420
IEAAEGNP S+SDRYLNQAAEI A GCKI G+SD LN DIL+ P FSKKLL+LI IL
Sbjct: 361 IEAAEGNPKYDSISDRYLNQAAEIFASGCKIDAGVSDMLNVDILKEPCFSKKLLRLIGIL 420
Query: 421 SSFRHRLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHI 480
SSFRHR NMKCIIFVNRIVIARSLSYILQNL+FLA+WKCD+LVGVHSKLRSMSRKTMNHI
Sbjct: 421 SSFRHRPNMKCIIFVNRIVIARSLSYILQNLKFLAYWKCDFLVGVHSKLRSMSRKTMNHI 480
Query: 481 LMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540
L KFRSGELNLL+ATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV
Sbjct: 481 LTKFRSGELNLLIATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLV 540
Query: 541 DSGNEKELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLL 600
DSGNEK+LGLIDEF KDE RM+REISSRSS E F+ HEESIYRV STGAS+TSGRSISLL
Sbjct: 541 DSGNEKDLGLIDEFHKDEKRMNREISSRSSIETFDGHEESIYRVVSTGASVTSGRSISLL 600
Query: 601 HEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACI 660
HE+CSKLPHD YFDPKPKFFY DDL GT+CHAILPSNAPI QI+SR QPS AKKDAC+
Sbjct: 601 HEYCSKLPHDTYFDPKPKFFYCDDLGGTICHAILPSNAPIPQIVSRPQPSGVAAKKDACL 660
Query: 661 KAIEELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDETSRGELHEMLVPAALKDSW 720
KA+EELHKLGAL+D+LL MRD+ANE ELELD SDSD SEDETSR ELHEM+ PAA K+SW
Sbjct: 661 KAVEELHKLGALSDYLLPMRDNANEGELELD-SDSDSSEDETSRLELHEMISPAAFKESW 720
Query: 721 TGSGYFILNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVP 780
TG GY IL CY IKF+P+P DRIYKEFGLFVKAPLPQEA RMD+ELHLA GRSV VNL+P
Sbjct: 721 TGFGYVILYCYHIKFTPNPEDRIYKEFGLFVKAPLPQEAVRMDLELHLAHGRSVKVNLMP 780
Query: 781 SGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLRNNVSKSISSTSYLLLPLIFHD 840
S VVELLE+EITQAE FQEMFLKVILDRSEFV ++IPLRNNVS+SISSTSYLLLP+I HD
Sbjct: 781 SRVVELLENEITQAECFQEMFLKVILDRSEFVHDYIPLRNNVSRSISSTSYLLLPVIVHD 840
Query: 841 NERSVSIDWKVIRRCLFSPIFQIPG-SIVKETACSHAHLTLYDGRRRTSDIKNSLVYVPY 900
NE SV IDWKVIRRCL S IFQ SI+KETA S HL LYDGRRR+SDI+NSLVYVPY
Sbjct: 841 NEGSVFIDWKVIRRCLSSQIFQNHACSIIKETASSDTHLMLYDGRRRSSDIENSLVYVPY 900
Query: 901 KREFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHN 960
KREFFFVT+IV+ KNGYS+ YK+S SLSHFEHL RFGIHLKYPEQPLL AKPLF LHN
Sbjct: 901 KREFFFVTNIVRRKNGYSQ--YKDSGSLSHFEHLKTRFGIHLKYPEQPLLCAKPLFSLHN 960
Query: 961 LLHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIE 1020
LLH+R+Q +S A YL+EYFID+ PELCQLKI GFSKDIGSSISLLPSIMHRLENLLVAIE
Sbjct: 961 LLHNRKQKDS-APYLEEYFIDVAPELCQLKITGFSKDIGSSISLLPSIMHRLENLLVAIE 1020
Query: 1021 LKCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDK 1080
LKCMLAAAFP GAEVTANRILEALTTEKCQER SLERLEILGD FLKFAVARYLFL HDK
Sbjct: 1021 LKCMLAAAFPAGAEVTANRILEALTTEKCQERFSLERLEILGDSFLKFAVARYLFLAHDK 1080
Query: 1081 SDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETRED 1140
DEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFY LGRPCPRICNEETR D
Sbjct: 1081 FDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYLLGRPCPRICNEETRND 1140
Query: 1141 IHSHCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIGIQ 1200
IHSH NAT NAK SETKCS+GHHWL KKTISD+VEAL+GAFLVDSGFKAAI+FLKWIGI+
Sbjct: 1141 IHSHDNATNNAKFSETKCSKGHHWLQKKTISDMVEALIGAFLVDSGFKAAIAFLKWIGIK 1200
Query: 1201 VEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGGGC 1260
VEFEAS VTNAL++S AY++ AD INIS LQ+SLGH+FLHKGLLLQALVHP+YHKHGGGC
Sbjct: 1201 VEFEASQVTNALVASKAYVLFADTINISALQSSLGHKFLHKGLLLQALVHPTYHKHGGGC 1260
Query: 1261 YQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYLIC 1320
YQRLEFLGDAVLDYLI SYLYS YPKLKPGLLTDLRSVFVRNEAFANVAVDR FYK+L+C
Sbjct: 1261 YQRLEFLGDAVLDYLIMSYLYSVYPKLKPGLLTDLRSVFVRNEAFANVAVDRFFYKFLLC 1320
Query: 1321 DSTSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVWKI 1380
DS SL+SDIKSYV+FIKAP S SL P CPK LGDLVES VGAVLVDTGFDMNYVWKI
Sbjct: 1321 DSASLESDIKSYVDFIKAPPSERDSLEQPRCPKPLGDLVESSVGAVLVDTGFDMNYVWKI 1380
Query: 1381 MLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHATAS 1440
MLSFLDPI+S SGFQLSPIRDIIE CQ+CG LQFN SK KYYSVEAEVKGD+FHATAS
Sbjct: 1381 MLSFLDPIMSSSGFQLSPIRDIIEVCQNCGWKLQFNPSKMEKYYSVEAEVKGDDFHATAS 1440
Query: 1441 AAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTIDQ 1500
A +RRK+DA+KIAAN IL KLKAQG IPEVNSLE+ILKSS KM+PKLIGYDETPSVT+D+
Sbjct: 1441 AVNRRKKDAEKIAANAILRKLKAQGFIPEVNSLEKILKSSEKMKPKLIGYDETPSVTMDR 1500
Query: 1501 ADNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAFD- 1560
D+ GLE SGADLDP VH EVGN+ P RITR+S+ L SSSGAA EQL PS+ +
Sbjct: 1501 IDD------GLEFSGADLDPLVHREVGNSRPFRITRMSETLASSSGAAGEQLKPSIPLEG 1560
Query: 1561 ---------------------AVSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPS 1620
+ S GR K TARSRLYE+CA NHW+ PSF+CMNEEGPS
Sbjct: 1561 HDSSTDLLSSSSSSSSSSSSSSSSSGRSGKTTARSRLYEVCAINHWNHPSFNCMNEEGPS 1620
Query: 1621 HLKMFTYKVVLEIEEAPNTIFKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYLPETD 1654
HLKMFTYKVVLEIEEAP+TIF+FFGAPHL KKAAAEHAAEAA+WYLKK YL +TD
Sbjct: 1621 HLKMFTYKVVLEIEEAPDTIFEFFGAPHLTKKAAAEHAAEAALWYLKKERYLGDTD 1657
BLAST of MS002366 vs. TAIR 10
Match:
AT5G20320.1 (dicer-like 4 )
HSP 1 Score: 1682.9 bits (4357), Expect = 0.0e+00
Identity = 892/1652 (54.00%), Postives = 1145/1652 (69.31%), Query Frame = 0
Query: 13 SDNFQHTSLGPVESFVG-----LSLVDDQEPGPSTRASEKDPRRKARKYQLELCKKALEE 72
S + TS V SF S +P P EKDPR+ AR+YQ+ELCKKA EE
Sbjct: 82 SSDLHLTSSSSVSSFSSSSSSLFSAAGTDDPSPK---MEKDPRKIARRYQVELCKKATEE 141
Query: 73 NIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIEDSTDFKV 132
N+I+YLGTGCGKTHIAV+L+YE+ HL+ +K +C+FLAPTVALV+QQAKVI DS +FKV
Sbjct: 142 NVIVYLGTGCGKTHIAVMLIYELGHLVLSPKKSVCIFLAPTVALVEQQAKVIADSVNFKV 201
Query: 133 RVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFDECHHAQV 192
++CGG + + SH +WERE+ +EV VMTP+ILL NL HC+IKME + LLIFDECHHAQ
Sbjct: 202 AIHCGGKRIVKSHSEWEREIAANEVLVMTPQILLHNLQHCFIKMECISLLIFDECHHAQQ 261
Query: 193 KSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLSKSINSLE 252
+S+H YAEIM+VFYK+ +RPRIFGMTASPVVGK G+ +NLSKSINSLE
Sbjct: 262 QSNHPYAEIMKVFYKSESLQRPRIFGMTASPVVGK---------GSFQSENLSKSINSLE 321
Query: 253 KLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKRQCIVVLG 312
LL+AKVYSVE +LD FVSSPLVK+YYY + S S + Y ++LED+K++C+ L
Sbjct: 322 NLLNAKVYSVESNVQLDGFVSSPLVKVYYYRSALSDASQSTIRYENMLEDIKQRCLASLK 381
Query: 313 QMKGEH--EDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAE 372
+ H + L + K+LL R HDN+I+ L NLG+WGA+QA+KI L+ D++ + E +
Sbjct: 382 LLIDTHQTQTLLSMKRLLKRSHDNLIYTLLNLGLWGAIQAAKIQLNSDHNVQDEPV---- 441
Query: 373 GNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRH 432
G + D YL+ AAE L+ G SD L+ L+ P FS+KL+QLI+ILS FR
Sbjct: 442 GKNPKSKICDTYLSMAAEALSSGVAKDENASDLLSLAALKEPLFSRKLVQLIKILSVFRL 501
Query: 433 RLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFR 492
+MKCIIFVNRIV AR+LS IL NLE L WK D+LVG+ S L+SMSR++M IL +F+
Sbjct: 502 EPHMKCIIFVNRIVTARTLSCILNNLELLRSWKSDFLVGLSSGLKSMSRRSMETILKRFQ 561
Query: 493 SGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNE 552
S ELNLLVATKVGEEGLDIQTCCLVIR+DLPETV+SFIQSRGRARMPQSEYAFLVDSGNE
Sbjct: 562 SKELNLLVATKVGEEGLDIQTCCLVIRYDLPETVTSFIQSRGRARMPQSEYAFLVDSGNE 621
Query: 553 KELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCS 612
KE+ LI+ F+ +E+RM+ EI+ RSS E +E +Y+V TGA I+ G SISLL+++CS
Sbjct: 622 KEMDLIENFKVNEDRMNLEITYRSSEETCPRLDEELYKVHETGACISGGSSISLLYKYCS 681
Query: 613 KLPHDEYFDPKPKFFY--YDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAI 672
+LPHDE+F PKP+F + D+ GT+C LP+NAPI +I S PS E AKKDAC+KA+
Sbjct: 682 RLPHDEFFQPKPEFQFKPVDEFGGTICRITLPANAPISEIESSLLPSTEAAKKDACLKAV 741
Query: 673 EELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDE-TSRGELHEMLVPAALKDSWTG 732
ELH LG LNDFLL D E+EL D D D + E SRG+L+EM VP K W
Sbjct: 742 HELHNLGVLNDFLLPDSKDEIEDELSDDEFDFDNIKGEGCSRGDLYEMRVPVLFKQKWDP 801
Query: 733 SGYFI-LNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVPS 792
S + L+ Y I F PHP DRIYK+FG F+K+PLP EAE MDI+LHLA RSV V + PS
Sbjct: 802 STSCVNLHSYYIMFVPHPADRIYKKFGFFMKSPLPVEAETMDIDLHLAHQRSVSVKIFPS 861
Query: 793 GVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLR-NNVSKSISSTSYLLLPLIFHD 852
GV E DEI AE FQE+ LKV+ +R E + +F+PL + S++ ST YLLLPL HD
Sbjct: 862 GVTEFDNDEIRLAELFQEIALKVLFERGELIPDFVPLELQDSSRTSKSTFYLLLPLCLHD 921
Query: 853 NERSVSIDWKVIRRCLFSPIFQIPGSIVKET-ACSHAHLTLYDGRRRTSDIKNSLVYVPY 912
E +S+DW IR CL SPIF+ P +V++ S +HL L +G D+KNSLV+ Y
Sbjct: 922 GESVISVDWVTIRNCLSSPIFKTPSVLVEDIFPPSGSHLKLANGCWNIDDVKNSLVFTTY 981
Query: 913 KREFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHN 972
++F+FV DI G+NG+ S K SS+ SH E + + +G+ LK+P QPLL KPL H+ N
Sbjct: 982 SKQFYFVADICHGRNGF--SPVKESSTKSHVESIYKLYGVELKHPAQPLLRVKPLCHVRN 1041
Query: 973 LLHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIE 1032
LLH+R Q N E L EYFI++PPEL LKI G SKDIGSS+SLLPSIMHR+ENLLVAIE
Sbjct: 1042 LLHNRMQTNLEPQELDEYFIEIPPELSHLKIKGLSKDIGSSLSLLPSIMHRMENLLVAIE 1101
Query: 1033 LKCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDK 1092
LK +L+A+ P+ AEV+ +R+LEALTTEKC ER+SLERLE+LGD FLKFAV+R+LFL HD
Sbjct: 1102 LKHVLSASIPEIAEVSGHRVLEALTTEKCHERLSLERLEVLGDAFLKFAVSRHLFLHHDS 1161
Query: 1093 SDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETRED 1152
DEGELTRRRS +V N NL +LA +KNLQVYIRDQ +P+QF+ G PC C+E ++
Sbjct: 1162 LDEGELTRRRSNVVNNSNLCRLAIKKNLQVYIRDQALDPTQFFAFGHPCRVTCDEVASKE 1221
Query: 1153 IHS--HCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIG 1212
+HS + E +CS+GHHWL+KKTI+DVVEALVGAFLVDSGFK A+ FLKWIG
Sbjct: 1222 VHSLNRDLGILESNTGEIRCSKGHHWLYKKTIADVVEALVGAFLVDSGFKGAVKFLKWIG 1281
Query: 1213 IQVEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGG 1272
+ V+FE+ V +A ++S Y+ L R N+ L+N L ++FLHKGLL+QA +HPSY++HGG
Sbjct: 1282 VNVDFESLQVQDACIASRRYLPLTTRNNLETLENQLDYKFLHKGLLVQAFIHPSYNRHGG 1341
Query: 1273 GCYQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYL 1332
GCYQRLEFLGDAVLDYL+TSY ++ +PKLKPG LTDLRS+ V NEA ANVAV S ++L
Sbjct: 1342 GCYQRLEFLGDAVLDYLMTSYFFTVFPKLKPGQLTDLRSLSVNNEALANVAVSFSLKRFL 1401
Query: 1333 ICDSTSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVW 1392
C+S L I+ Y NF+ + SG P CPK LGDLVESC+GA+ +D GF++N+VW
Sbjct: 1402 FCESIYLHEVIEDYTNFLASSPLASGQSEGPRCPKVLGDLVESCLGALFLDCGFNLNHVW 1461
Query: 1393 KIMLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHAT 1452
+MLSFLDP+ + S Q+SPI+++IE CQ + + + +K ++VE +V + T
Sbjct: 1462 TMMLSFLDPVKNLSNLQISPIKELIELCQSYKWDREISATKKDGAFTVELKVTKNGCCLT 1521
Query: 1453 ASAAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTI 1512
SA R KR+ K AA L++T LKA I + LE++LK+ + E KLIGY+E P +
Sbjct: 1522 VSATGRNKREGTKKAAQLMITNLKAHENITTSHPLEDVLKNGIRNEAKLIGYNEDPIDVV 1581
Query: 1513 DQADNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAF 1572
D + LE+ G GN+ + I + L + +++L P A
Sbjct: 1582 DLVGLDVENLNILETFG-----------GNSERSSSYVIRRGLPQAPSKTEDRL-PQKAI 1641
Query: 1573 DAVSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEAPNTI 1632
+ G TA+S L+E C AN W PP F+C EEGP HLK F YKV+LE+E+APN
Sbjct: 1642 --IKAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMT 1701
Query: 1633 FKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYL 1650
+ +G KK AAEHAA+AAIW LK +G+L
Sbjct: 1702 LECYGEARATKKGAAEHAAQAAIWCLKHSGFL 1701
BLAST of MS002366 vs. TAIR 10
Match:
AT5G20320.2 (dicer-like 4 )
HSP 1 Score: 1654.4 bits (4283), Expect = 0.0e+00
Identity = 882/1652 (53.39%), Postives = 1134/1652 (68.64%), Query Frame = 0
Query: 13 SDNFQHTSLGPVESFVG-----LSLVDDQEPGPSTRASEKDPRRKARKYQLELCKKALEE 72
S + TS V SF S +P P EKDPR+ AR+YQ+ELCKKA EE
Sbjct: 82 SSDLHLTSSSSVSSFSSSSSSLFSAAGTDDPSPK---MEKDPRKIARRYQVELCKKATEE 141
Query: 73 NIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPTVALVQQQAKVIEDSTDFKV 132
N+I+YLGTGCGKTHIAV+L+YE+ HL+ +K +C+FLAPTVALV+QQAKVI DS +FKV
Sbjct: 142 NVIVYLGTGCGKTHIAVMLIYELGHLVLSPKKSVCIFLAPTVALVEQQAKVIADSVNFKV 201
Query: 133 RVYCGGSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKMEFVELLIFDECHHAQV 192
++CGG + + SH +WERE+ +E HC+IKME + LLIFDECHHAQ
Sbjct: 202 AIHCGGKRIVKSHSEWEREIAANE--------------HCFIKMECISLLIFDECHHAQQ 261
Query: 193 KSDHSYAEIMRVFYKTNVAKRPRIFGMTASPVVGKDHCSTSTNSGACNQQNLSKSINSLE 252
+S+H YAEIM+VFYK+ +RPRIFGMTASPVVGK G+ +NLSKSINSLE
Sbjct: 262 QSNHPYAEIMKVFYKSESLQRPRIFGMTASPVVGK---------GSFQSENLSKSINSLE 321
Query: 253 KLLDAKVYSVEDREELDAFVSSPLVKIYYYGPVANGLSGSFMTYCSLLEDMKRQCIVVLG 312
LL+AKVYSVE +LD FVSSPLVK+YYY + S S + Y ++LED+K++C+ L
Sbjct: 322 NLLNAKVYSVESNVQLDGFVSSPLVKVYYYRSALSDASQSTIRYENMLEDIKQRCLASLK 381
Query: 313 QMKGEH--EDLRATKKLLCRMHDNIIFCLENLGVWGALQASKILLSGDNSERSELIEAAE 372
+ H + L + K+LL R HDN+I+ L NLG+WGA+QA+KI L+ D++ + E +
Sbjct: 382 LLIDTHQTQTLLSMKRLLKRSHDNLIYTLLNLGLWGAIQAAKIQLNSDHNVQDEPV---- 441
Query: 373 GNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNADILEYPFFSKKLLQLIEILSSFRH 432
G + D YL+ AAE L+ G SD L+ L+ P FS+KL+QLI+ILS FR
Sbjct: 442 GKNPKSKICDTYLSMAAEALSSGVAKDENASDLLSLAALKEPLFSRKLVQLIKILSVFRL 501
Query: 433 RLNMKCIIFVNRIVIARSLSYILQNLEFLAFWKCDYLVGVHSKLRSMSRKTMNHILMKFR 492
+MKCIIFVNRIV AR+LS IL NLE L WK D+LVG+ S L+SMSR++M IL +F+
Sbjct: 502 EPHMKCIIFVNRIVTARTLSCILNNLELLRSWKSDFLVGLSSGLKSMSRRSMETILKRFQ 561
Query: 493 SGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSSFIQSRGRARMPQSEYAFLVDSGNE 552
S ELNLLVATKVGEEGLDIQTCCLVIR+DLPETV+SFIQSRGRARMPQSEYAFLVDSGNE
Sbjct: 562 SKELNLLVATKVGEEGLDIQTCCLVIRYDLPETVTSFIQSRGRARMPQSEYAFLVDSGNE 621
Query: 553 KELGLIDEFQKDENRMDREISSRSSSEIFNSHEESIYRVASTGASITSGRSISLLHEFCS 612
KE+ LI+ F+ +E+RM+ EI+ RSS E +E +Y+V TGA I+ G SISLL+++CS
Sbjct: 622 KEMDLIENFKVNEDRMNLEITYRSSEETCPRLDEELYKVHETGACISGGSSISLLYKYCS 681
Query: 613 KLPHDEYFDPKPKFFY--YDDLAGTVCHAILPSNAPIHQIISRTQPSREGAKKDACIKAI 672
+LPHDE+F PKP+F + D+ GT+C LP+NAPI +I S PS E AKKDAC+KA+
Sbjct: 682 RLPHDEFFQPKPEFQFKPVDEFGGTICRITLPANAPISEIESSLLPSTEAAKKDACLKAV 741
Query: 673 EELHKLGALNDFLLLMRDDANEEELELDSSDSDRSEDE-TSRGELHEMLVPAALKDSWTG 732
ELH LG LNDFLL D E+EL D D D + E SRG+L+EM VP K W
Sbjct: 742 HELHNLGVLNDFLLPDSKDEIEDELSDDEFDFDNIKGEGCSRGDLYEMRVPVLFKQKWDP 801
Query: 733 SGYFI-LNCYQIKFSPHPRDRIYKEFGLFVKAPLPQEAERMDIELHLARGRSVMVNLVPS 792
S + L+ Y I F PHP DRIYK+FG F+K+PLP EAE MDI+LHLA RSV V + PS
Sbjct: 802 STSCVNLHSYYIMFVPHPADRIYKKFGFFMKSPLPVEAETMDIDLHLAHQRSVSVKIFPS 861
Query: 793 GVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIPLR-NNVSKSISSTSYLLLPLIFHD 852
GV E DEI AE FQE+ LKV+ +R E + +F+PL + S++ ST YLLLPL HD
Sbjct: 862 GVTEFDNDEIRLAELFQEIALKVLFERGELIPDFVPLELQDSSRTSKSTFYLLLPLCLHD 921
Query: 853 NERSVSIDWKVIRRCLFSPIFQIPGSIVKET-ACSHAHLTLYDGRRRTSDIKNSLVYVPY 912
E +S+DW IR CL SPIF+ P +V++ S +HL L +G D+KNSLV+ Y
Sbjct: 922 GESVISVDWVTIRNCLSSPIFKTPSVLVEDIFPPSGSHLKLANGCWNIDDVKNSLVFTTY 981
Query: 913 KREFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIERFGIHLKYPEQPLLSAKPLFHLHN 972
++F+FV DI G+NG+ S K SS+ SH E + + +G+ LK+P QPLL KPL H+ N
Sbjct: 982 SKQFYFVADICHGRNGF--SPVKESSTKSHVESIYKLYGVELKHPAQPLLRVKPLCHVRN 1041
Query: 973 LLHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKDIGSSISLLPSIMHRLENLLVAIE 1032
LLH+R Q N E L EYFI++PPEL LKI G SKDIGSS+SLLPSIMHR+ENLLVAIE
Sbjct: 1042 LLHNRMQTNLEPQELDEYFIEIPPELSHLKIKGLSKDIGSSLSLLPSIMHRMENLLVAIE 1101
Query: 1033 LKCMLAAAFPDGAEVTANRILEALTTEKCQERISLERLEILGDGFLKFAVARYLFLTHDK 1092
LK +L+A+ P+ AEV+ +R+LEALTTEKC ER+SLERLE+LGD FLKFAV+R+LFL HD
Sbjct: 1102 LKHVLSASIPEIAEVSGHRVLEALTTEKCHERLSLERLEVLGDAFLKFAVSRHLFLHHDS 1161
Query: 1093 SDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPFEPSQFYTLGRPCPRICNEETRED 1152
DEGELTRRRS +V N NL +LA +KNLQVYIRDQ +P+QF+ G PC C+E ++
Sbjct: 1162 LDEGELTRRRSNVVNNSNLCRLAIKKNLQVYIRDQALDPTQFFAFGHPCRVTCDEVASKE 1221
Query: 1153 IHS--HCNATKNAKASETKCSRGHHWLHKKTISDVVEALVGAFLVDSGFKAAISFLKWIG 1212
+HS + E +CS+GHHWL+KKTI+DVVEALVGAFLVDSGFK A+ FLKWIG
Sbjct: 1222 VHSLNRDLGILESNTGEIRCSKGHHWLYKKTIADVVEALVGAFLVDSGFKGAVKFLKWIG 1281
Query: 1213 IQVEFEASLVTNALMSSNAYIILADRINISVLQNSLGHQFLHKGLLLQALVHPSYHKHGG 1272
+ V+FE+ V +A ++S Y+ L R N+ L+N L ++FLHKGLL+QA +HPSY++HGG
Sbjct: 1282 VNVDFESLQVQDACIASRRYLPLTTRNNLETLENQLDYKFLHKGLLVQAFIHPSYNRHGG 1341
Query: 1273 GCYQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTDLRSVFVRNEAFANVAVDRSFYKYL 1332
GCYQRLEFLGDAVLDYL+TSY ++ +PKLKPG LTDLRS+ V NEA ANVAV S ++L
Sbjct: 1342 GCYQRLEFLGDAVLDYLMTSYFFTVFPKLKPGQLTDLRSLSVNNEALANVAVSFSLKRFL 1401
Query: 1333 ICDSTSLQSDIKSYVNFIKAPSSGSGSLGLPSCPKALGDLVESCVGAVLVDTGFDMNYVW 1392
C+S L I+ Y NF+ + SG P CPK LGDLVESC+GA+ +D GF++N+VW
Sbjct: 1402 FCESIYLHEVIEDYTNFLASSPLASGQSEGPRCPKVLGDLVESCLGALFLDCGFNLNHVW 1461
Query: 1393 KIMLSFLDPIISFSGFQLSPIRDIIEFCQDCGSNLQFNESKTGKYYSVEAEVKGDNFHAT 1452
+MLSFLDP+ + S Q+SPI+++IE CQ + + + +K ++VE +V + T
Sbjct: 1462 TMMLSFLDPVKNLSNLQISPIKELIELCQSYKWDREISATKKDGAFTVELKVTKNGCCLT 1521
Query: 1453 ASAAHRRKRDAKKIAANLILTKLKAQGLIPEVNSLEEILKSSRKMEPKLIGYDETPSVTI 1512
SA R KR+ K AA L++T LKA I + LE++LK+ + E KLIGY+E P +
Sbjct: 1522 VSATGRNKREGTKKAAQLMITNLKAHENITTSHPLEDVLKNGIRNEAKLIGYNEDPIDVV 1581
Query: 1513 DQADNGFGTIKGLESSGADLDPFVHSEVGNAHPARITRISKMLVSSSGAAQEQLDPSLAF 1572
D + LE+ G GN+ + I + L + +++L P A
Sbjct: 1582 DLVGLDVENLNILETFG-----------GNSERSSSYVIRRGLPQAPSKTEDRL-PQKAI 1641
Query: 1573 DAVSLGRLCKATARSRLYEICAANHWSPPSFDCMNEEGPSHLKMFTYKVVLEIEEAPNTI 1632
+ G TA+S L+E C AN W PP F+C EEGP HLK F YKV+LE+E+APN
Sbjct: 1642 --IKAGGPSSKTAKSLLHETCVANCWKPPHFECCEEEGPGHLKSFVYKVILEVEDAPNMT 1687
Query: 1633 FKFFGAPHLRKKAAAEHAAEAAIWYLKKAGYL 1650
+ +G KK AAEHAA+AAIW LK +G+L
Sbjct: 1702 LECYGEARATKKGAAEHAAQAAIWCLKHSGFL 1687
BLAST of MS002366 vs. TAIR 10
Match:
AT3G03300.1 (dicer-like 2 )
HSP 1 Score: 661.8 bits (1706), Expect = 1.5e-189
Identity = 472/1435 (32.89%), Postives = 746/1435 (51.99%), Query Frame = 0
Query: 49 PRRKARKYQLELCKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPT 108
P AR YQ+E +KA+++N I++L TG GKT IA++L+ +L R+ CVFL P
Sbjct: 20 PLHFARSYQVEALEKAIKQNTIVFLETGSGKTLIAIMLLRSYAYLFRKPSPCFCVFLVPQ 79
Query: 109 VALVQQQAKVIEDSTDFKVRVYCG--GSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHH 168
V LV QQA+ ++ TD KV +Y G G +S W++E++++EV VMTP ILL L H
Sbjct: 80 VVLVTQQAEALKMHTDLKVGMYWGDMGVDFWDSS-TWKQEVDKYEVLVMTPAILLDALRH 139
Query: 169 CYIKMEFVELLIFDECHHAQVKSDHSYAEIMRVFY----KTNVAKRPRIFGMTASPVVGK 228
++ + +++LI DECHHA K H YA IMR FY + + PRIFGMTAS V K
Sbjct: 140 SFLSLSMIKVLIVDECHHAGGK--HPYACIMREFYHKELNSGTSNVPRIFGMTASLVKTK 199
Query: 229 DHCSTSTNSGACNQQNLSKSINSLEKLLDAKVYSVEDREELDAFV--SSPLVKIYYYGPV 288
N + K I+ LE L+++KVY+ E+ L FV S+P K Y + +
Sbjct: 200 GE----------NLDSYWKKIHELETLMNSKVYTCENESVLAGFVPFSTPSFKYYQHIKI 259
Query: 289 ANGLSGSFMTYCSLLEDMKRQCI---VVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENL 348
+ SL+E ++R I + LG + + + +K L R+ + +CL++L
Sbjct: 260 PS------PKRASLVEKLERLTIKHRLSLGTLDLNSSTVDSVEKRLLRISSTLTYCLDDL 319
Query: 349 GVWGALQASKILLSGDNSERSELIEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSD 408
G+ A +A++ L + N + E N + +L ++ + A++ G S
Sbjct: 320 GILLAQKAAQSLSASQN----DSFLWGELNMFSVALVKKFCSDASQEFLAEIPQGLNWSV 379
Query: 409 TLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCIIFVNRIVIARSL-SYILQNLEFLAF 468
E + K + LIE L + N++CIIFV+R++ A L S + + L
Sbjct: 380 ANINGNAEAGLLTLKTVCLIETLLGYSSLENIRCIIFVDRVITAIVLESLLAEILPNCNN 439
Query: 469 WKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLP 528
WK Y+ G +S L++ +RK N I+ FR G +N++VAT + EEGLD+Q+C LVIRFD
Sbjct: 440 WKTKYVAGNNSGLQNQTRKKQNEIVEDFRRGLVNIIVATSILEEGLDVQSCNLVIRFDPA 499
Query: 529 ETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLIDEFQKDENRMDREISSRS---SSEI 588
+ SFIQSRGRARM S+Y +V+SG+ + ++ RM E S +
Sbjct: 500 SNICSFIQSRGRARMQNSDYLMMVESGDLLTQSRLMKYLSGGKRMREESLDHSLVPCPPL 559
Query: 589 FNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAI 648
+ +E ++RV STGA++T S+SL++ +CS+LP DEYF P P+F D C
Sbjct: 560 PDDSDEPLFRVESTGATVTLSSSVSLIYHYCSRLPSDEYFKPAPRFDVNKDQGS--CTLY 619
Query: 649 LPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGALNDFLLLMRDDANEEELELDSS 708
LP + P+ ++ + + + + K+ C+KA +LHK+GAL+D L+ ++ + +
Sbjct: 620 LPKSCPVKEV--KAEANNKVLKQAVCLKACIQLHKVGALSDHLV--------PDMVVAET 679
Query: 709 DSDRSEDETSRGELHEMLVPAALKDSWTGSGYFILNCYQIKFSPH-PRDRIYKEFGLFVK 768
S + E E P L ++ + Y I+ P+ PR+ + L +
Sbjct: 680 VSQKLEKIQYNTE-QPCYFPPELVSQFSAQPETTYHFYLIRMKPNSPRNFHLNDVLLGTR 739
Query: 769 APLPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEITQAESFQEMFLKVILDRSEFV 828
L + L RG ++ V L G L ++E+ FQ +V+LD S V
Sbjct: 740 VVLEDDIGNTSFRLEDHRG-TIAVTLSYVGAFHLTQEEVLFCRRFQITLFRVLLDHS--V 799
Query: 829 QEFIPLRNNVS-KSISSTSYLLLPLIFHDNERSVSIDWKVIRRCLFSPIFQIPGSIVKET 888
+ + N + + + YLL+P H +E S+ IDW+VIR + + T
Sbjct: 800 ENLMEALNGLHLRDGVALDYLLVPST-HSHETSL-IDWEVIRSVNLTSHEVLEKHENCST 859
Query: 889 ACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKGKNGYSRSHYKNSSSLSHFE 948
+ L DG T ++N+LVY P+ + ++ NG S +NS ++ E
Sbjct: 860 NGASRILHTKDGLFCTCVVQNALVYTPHNGYVYCTKGVLNNLNGNSLLTKRNSGDQTYIE 919
Query: 949 HLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEACYLQEYFIDLPPELCQLKII 1008
+ ER GI L + ++PLL+ + +F LH+ LH ++ + F++LPPELC + +
Sbjct: 920 YYEERHGIQLNFVDEPLLNGRHIFTLHSYLHMAKKKKEKE--HDREFVELPPELCHVILS 979
Query: 1009 GFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGAEVTANRILEALTTEKCQER 1068
S D+ S + +PS+M R+E+LL+A LK + + ++LEA+TT+KC+++
Sbjct: 980 PISVDMIYSYTFIPSVMQRIESLLIAYNLKKSIPK-----VNIPTIKVLEAITTKKCEDQ 1039
Query: 1069 ISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYLVKNINLFKLATRKNLQVYI 1128
LE LE LGD FLK+AV + LF EG L+ ++ ++ N+ L + ++ LQ +I
Sbjct: 1040 FHLESLETLGDSFLKYAVCQQLFQHCHTHHEGLLSTKKDGMISNVMLCQFGCQQKLQGFI 1099
Query: 1129 RDQPFEPSQFYTLGRPCP--RICNEETREDIHSHCNATKNAKASETKCSRGHHWLHKKTI 1188
RD+ FEP + G+ + N+ E + + + +N K +K++
Sbjct: 1100 RDECFEPKGWMVPGQSSAAYSLVNDTLPESRNIYVASRRNLK--------------RKSV 1159
Query: 1189 SDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALMSSNAYIILADRINISVL 1248
+DVVE+L+GA+L + G AA+ F+ W+GI+V+F T + ++ I +N+ +
Sbjct: 1160 ADVVESLIGAYLSEGGELAALMFMNWVGIKVDF-----TTTKIQRDSPIQAEKLVNVGYM 1219
Query: 1249 QNSLGHQFLHKGLLLQALVHPSY-HKHGGGCYQRLEFLGDAVLDYLITSYLYSAYPKLKP 1308
++ L + F K LL++AL H SY CYQRLEFLGD+VLDYLIT +LY YP L P
Sbjct: 1220 ESLLNYSFEDKSLLVEALTHGSYMMPEIPRCYQRLEFLGDSVLDYLITKHLYDKYPCLSP 1279
Query: 1309 GLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYVNFIKAPS--SGSGSLG 1368
GLLTD+RS V NE +A VAV + +K+++ S L I V+ + S S G
Sbjct: 1280 GLLTDMRSASVNNECYALVAVKANLHKHILYASHHLHKHISRTVSEFEQSSLQSTFGWES 1339
Query: 1369 LPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSGFQLSPIRDIIEFCQ 1428
S PK LGD++ES GA+ VD+G++ V+ + L +I+ +L P+R++ E CQ
Sbjct: 1340 DISFPKVLGDVIESLAGAIFVDSGYNKEVVFASIKPLLGCMITPETVKLHPVRELTELCQ 1383
Query: 1429 DCGSNLQFNESKTGKYYSVEAEVKGDNFHATASAAHRRKRDAKKIAANLILTKLK 1462
QF SK + S EVK +A K+ AKK+A +L LK
Sbjct: 1400 ----KWQFELSKAKDFDSFTVEVKAKEMSFAHTAKASDKKMAKKLAYKEVLNLLK 1383
BLAST of MS002366 vs. TAIR 10
Match:
AT3G03300.3 (dicer-like 2 )
HSP 1 Score: 661.8 bits (1706), Expect = 1.5e-189
Identity = 472/1435 (32.89%), Postives = 746/1435 (51.99%), Query Frame = 0
Query: 49 PRRKARKYQLELCKKALEENIILYLGTGCGKTHIAVLLMYEMRHLIRRSQKGICVFLAPT 108
P AR YQ+E +KA+++N I++L TG GKT IA++L+ +L R+ CVFL P
Sbjct: 20 PLHFARSYQVEALEKAIKQNTIVFLETGSGKTLIAIMLLRSYAYLFRKPSPCFCVFLVPQ 79
Query: 109 VALVQQQAKVIEDSTDFKVRVYCG--GSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHH 168
V LV QQA+ ++ TD KV +Y G G +S W++E++++EV VMTP ILL L H
Sbjct: 80 VVLVTQQAEALKMHTDLKVGMYWGDMGVDFWDSS-TWKQEVDKYEVLVMTPAILLDALRH 139
Query: 169 CYIKMEFVELLIFDECHHAQVKSDHSYAEIMRVFY----KTNVAKRPRIFGMTASPVVGK 228
++ + +++LI DECHHA K H YA IMR FY + + PRIFGMTAS V K
Sbjct: 140 SFLSLSMIKVLIVDECHHAGGK--HPYACIMREFYHKELNSGTSNVPRIFGMTASLVKTK 199
Query: 229 DHCSTSTNSGACNQQNLSKSINSLEKLLDAKVYSVEDREELDAFV--SSPLVKIYYYGPV 288
N + K I+ LE L+++KVY+ E+ L FV S+P K Y + +
Sbjct: 200 GE----------NLDSYWKKIHELETLMNSKVYTCENESVLAGFVPFSTPSFKYYQHIKI 259
Query: 289 ANGLSGSFMTYCSLLEDMKRQCI---VVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENL 348
+ SL+E ++R I + LG + + + +K L R+ + +CL++L
Sbjct: 260 PS------PKRASLVEKLERLTIKHRLSLGTLDLNSSTVDSVEKRLLRISSTLTYCLDDL 319
Query: 349 GVWGALQASKILLSGDNSERSELIEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSD 408
G+ A +A++ L + N + E N + +L ++ + A++ G S
Sbjct: 320 GILLAQKAAQSLSASQN----DSFLWGELNMFSVALVKKFCSDASQEFLAEIPQGLNWSV 379
Query: 409 TLNADILEYPFFSKKLLQLIEILSSFRHRLNMKCIIFVNRIVIARSL-SYILQNLEFLAF 468
E + K + LIE L + N++CIIFV+R++ A L S + + L
Sbjct: 380 ANINGNAEAGLLTLKTVCLIETLLGYSSLENIRCIIFVDRVITAIVLESLLAEILPNCNN 439
Query: 469 WKCDYLVGVHSKLRSMSRKTMNHILMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLP 528
WK Y+ G +S L++ +RK N I+ FR G +N++VAT + EEGLD+Q+C LVIRFD
Sbjct: 440 WKTKYVAGNNSGLQNQTRKKQNEIVEDFRRGLVNIIVATSILEEGLDVQSCNLVIRFDPA 499
Query: 529 ETVSSFIQSRGRARMPQSEYAFLVDSGNEKELGLIDEFQKDENRMDREISSRS---SSEI 588
+ SFIQSRGRARM S+Y +V+SG+ + ++ RM E S +
Sbjct: 500 SNICSFIQSRGRARMQNSDYLMMVESGDLLTQSRLMKYLSGGKRMREESLDHSLVPCPPL 559
Query: 589 FNSHEESIYRVASTGASITSGRSISLLHEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAI 648
+ +E ++RV STGA++T S+SL++ +CS+LP DEYF P P+F D C
Sbjct: 560 PDDSDEPLFRVESTGATVTLSSSVSLIYHYCSRLPSDEYFKPAPRFDVNKDQGS--CTLY 619
Query: 649 LPSNAPIHQIISRTQPSREGAKKDACIKAIEELHKLGALNDFLLLMRDDANEEELELDSS 708
LP + P+ ++ + + + + K+ C+KA +LHK+GAL+D L+ ++ + +
Sbjct: 620 LPKSCPVKEV--KAEANNKVLKQAVCLKACIQLHKVGALSDHLV--------PDMVVAET 679
Query: 709 DSDRSEDETSRGELHEMLVPAALKDSWTGSGYFILNCYQIKFSPH-PRDRIYKEFGLFVK 768
S + E E P L ++ + Y I+ P+ PR+ + L +
Sbjct: 680 VSQKLEKIQYNTE-QPCYFPPELVSQFSAQPETTYHFYLIRMKPNSPRNFHLNDVLLGTR 739
Query: 769 APLPQEAERMDIELHLARGRSVMVNLVPSGVVELLEDEITQAESFQEMFLKVILDRSEFV 828
L + L RG ++ V L G L ++E+ FQ +V+LD S V
Sbjct: 740 VVLEDDIGNTSFRLEDHRG-TIAVTLSYVGAFHLTQEEVLFCRRFQITLFRVLLDHS--V 799
Query: 829 QEFIPLRNNVS-KSISSTSYLLLPLIFHDNERSVSIDWKVIRRCLFSPIFQIPGSIVKET 888
+ + N + + + YLL+P H +E S+ IDW+VIR + + T
Sbjct: 800 ENLMEALNGLHLRDGVALDYLLVPST-HSHETSL-IDWEVIRSVNLTSHEVLEKHENCST 859
Query: 889 ACSHAHLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKGKNGYSRSHYKNSSSLSHFE 948
+ L DG T ++N+LVY P+ + ++ NG S +NS ++ E
Sbjct: 860 NGASRILHTKDGLFCTCVVQNALVYTPHNGYVYCTKGVLNNLNGNSLLTKRNSGDQTYIE 919
Query: 949 HLIERFGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEACYLQEYFIDLPPELCQLKII 1008
+ ER GI L + ++PLL+ + +F LH+ LH ++ + F++LPPELC + +
Sbjct: 920 YYEERHGIQLNFVDEPLLNGRHIFTLHSYLHMAKKKKEKE--HDREFVELPPELCHVILS 979
Query: 1009 GFSKDIGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGAEVTANRILEALTTEKCQER 1068
S D+ S + +PS+M R+E+LL+A LK + + ++LEA+TT+KC+++
Sbjct: 980 PISVDMIYSYTFIPSVMQRIESLLIAYNLKKSIPK-----VNIPTIKVLEAITTKKCEDQ 1039
Query: 1069 ISLERLEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYLVKNINLFKLATRKNLQVYI 1128
LE LE LGD FLK+AV + LF EG L+ ++ ++ N+ L + ++ LQ +I
Sbjct: 1040 FHLESLETLGDSFLKYAVCQQLFQHCHTHHEGLLSTKKDGMISNVMLCQFGCQQKLQGFI 1099
Query: 1129 RDQPFEPSQFYTLGRPCP--RICNEETREDIHSHCNATKNAKASETKCSRGHHWLHKKTI 1188
RD+ FEP + G+ + N+ E + + + +N K +K++
Sbjct: 1100 RDECFEPKGWMVPGQSSAAYSLVNDTLPESRNIYVASRRNLK--------------RKSV 1159
Query: 1189 SDVVEALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALMSSNAYIILADRINISVL 1248
+DVVE+L+GA+L + G AA+ F+ W+GI+V+F T + ++ I +N+ +
Sbjct: 1160 ADVVESLIGAYLSEGGELAALMFMNWVGIKVDF-----TTTKIQRDSPIQAEKLVNVGYM 1219
Query: 1249 QNSLGHQFLHKGLLLQALVHPSY-HKHGGGCYQRLEFLGDAVLDYLITSYLYSAYPKLKP 1308
++ L + F K LL++AL H SY CYQRLEFLGD+VLDYLIT +LY YP L P
Sbjct: 1220 ESLLNYSFEDKSLLVEALTHGSYMMPEIPRCYQRLEFLGDSVLDYLITKHLYDKYPCLSP 1279
Query: 1309 GLLTDLRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYVNFIKAPS--SGSGSLG 1368
GLLTD+RS V NE +A VAV + +K+++ S L I V+ + S S G
Sbjct: 1280 GLLTDMRSASVNNECYALVAVKANLHKHILYASHHLHKHISRTVSEFEQSSLQSTFGWES 1339
Query: 1369 LPSCPKALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSGFQLSPIRDIIEFCQ 1428
S PK LGD++ES GA+ VD+G++ V+ + L +I+ +L P+R++ E CQ
Sbjct: 1340 DISFPKVLGDVIESLAGAIFVDSGYNKEVVFASIKPLLGCMITPETVKLHPVRELTELCQ 1383
Query: 1429 DCGSNLQFNESKTGKYYSVEAEVKGDNFHATASAAHRRKRDAKKIAANLILTKLK 1462
QF SK + S EVK +A K+ AKK+A +L LK
Sbjct: 1400 ----KWQFELSKAKDFDSFTVEVKAKEMSFAHTAKASDKKMAKKLAYKEVLNLLK 1383
BLAST of MS002366 vs. TAIR 10
Match:
AT3G03300.2 (dicer-like 2 )
HSP 1 Score: 607.8 bits (1566), Expect = 2.5e-173
Identity = 443/1370 (32.34%), Postives = 705/1370 (51.46%), Query Frame = 0
Query: 114 QQAKVIEDSTDFKVRVYCG--GSKCLNSHYDWEREMEEHEVFVMTPEILLRNLHHCYIKM 173
QQA+ ++ TD KV +Y G G +S W++E++++EV VMTP ILL L H ++ +
Sbjct: 71 QQAEALKMHTDLKVGMYWGDMGVDFWDSS-TWKQEVDKYEVLVMTPAILLDALRHSFLSL 130
Query: 174 EFVELLIFDECHHAQVKSDHSYAEIMRVFY----KTNVAKRPRIFGMTASPVVGKDHCST 233
+++LI DECHHA K H YA IMR FY + + PRIFGMTAS V K
Sbjct: 131 SMIKVLIVDECHHAGGK--HPYACIMREFYHKELNSGTSNVPRIFGMTASLVKTKGE--- 190
Query: 234 STNSGACNQQNLSKSINSLEKLLDAKVYSVEDREELDAFV--SSPLVKIYYYGPVANGLS 293
N + K I+ LE L+++KVY+ E+ L FV S+P K Y + + +
Sbjct: 191 -------NLDSYWKKIHELETLMNSKVYTCENESVLAGFVPFSTPSFKYYQHIKIPS--- 250
Query: 294 GSFMTYCSLLEDMKRQCI---VVLGQMKGEHEDLRATKKLLCRMHDNIIFCLENLGVWGA 353
SL+E ++R I + LG + + + +K L R+ + +CL++LG+ A
Sbjct: 251 ---PKRASLVEKLERLTIKHRLSLGTLDLNSSTVDSVEKRLLRISSTLTYCLDDLGILLA 310
Query: 354 LQASKILLSGDNSERSELIEAAEGNPNNHSLSDRYLNQAAEILAKGCKIGGGMSDTLNAD 413
+A++ L + N + E N + +L ++ + A++ G S
Sbjct: 311 QKAAQSLSASQN----DSFLWGELNMFSVALVKKFCSDASQEFLAEIPQGLNWSVANING 370
Query: 414 ILEYPFFSKKLLQLIEILSSFRHRLNMKCIIFVNRIVIARSL-SYILQNLEFLAFWKCDY 473
E + K + LIE L + N++CIIFV+R++ A L S + + L WK Y
Sbjct: 371 NAEAGLLTLKTVCLIETLLGYSSLENIRCIIFVDRVITAIVLESLLAEILPNCNNWKTKY 430
Query: 474 LVGVHSKLRSMSRKTMNHILMKFRSGELNLLVATKVGEEGLDIQTCCLVIRFDLPETVSS 533
+ G +S L++ +RK N I+ FR G +N++VAT + EEGLD+Q+C LVIRFD + S
Sbjct: 431 VAGNNSGLQNQTRKKQNEIVEDFRRGLVNIIVATSILEEGLDVQSCNLVIRFDPASNICS 490
Query: 534 FIQSRGRARMPQSEYAFLVDSGNEKELGLIDEFQKDENRMDREISSRS---SSEIFNSHE 593
FIQSRGRARM S+Y +V+SG+ + ++ RM E S + + +
Sbjct: 491 FIQSRGRARMQNSDYLMMVESGDLLTQSRLMKYLSGGKRMREESLDHSLVPCPPLPDDSD 550
Query: 594 ESIYRVASTGASITSGRSISLLHEFCSKLPHDEYFDPKPKFFYYDDLAGTVCHAILPSNA 653
E ++RV STGA++T S+SL++ +CS+LP DEYF P P+F D C LP +
Sbjct: 551 EPLFRVESTGATVTLSSSVSLIYHYCSRLPSDEYFKPAPRFDVNKDQGS--CTLYLPKSC 610
Query: 654 PIHQIISRTQPSREGAKKDACIKAIEELHKLGALNDFLLLMRDDANEEELELDSSDSDRS 713
P+ ++ + + + + K+ C+KA +LHK+GAL+D L+ ++ + + S +
Sbjct: 611 PVKEV--KAEANNKVLKQAVCLKACIQLHKVGALSDHLV--------PDMVVAETVSQKL 670
Query: 714 EDETSRGELHEMLVPAALKDSWTGSGYFILNCYQIKFSPH-PRDRIYKEFGLFVKAPLPQ 773
E E P L ++ + Y I+ P+ PR+ + L + L
Sbjct: 671 EKIQYNTE-QPCYFPPELVSQFSAQPETTYHFYLIRMKPNSPRNFHLNDVLLGTRVVLED 730
Query: 774 EAERMDIELHLARGRSVMVNLVPSGVVELLEDEITQAESFQEMFLKVILDRSEFVQEFIP 833
+ L RG ++ V L G L ++E+ FQ +V+LD S V+ +
Sbjct: 731 DIGNTSFRLEDHRG-TIAVTLSYVGAFHLTQEEVLFCRRFQITLFRVLLDHS--VENLME 790
Query: 834 LRNNVS-KSISSTSYLLLPLIFHDNERSVSIDWKVIRRCLFSPIFQIPGSIVKETACSHA 893
N + + + YLL+P H +E S+ IDW+VIR + + T +
Sbjct: 791 ALNGLHLRDGVALDYLLVPST-HSHETSL-IDWEVIRSVNLTSHEVLEKHENCSTNGASR 850
Query: 894 HLTLYDGRRRTSDIKNSLVYVPYKREFFFVTDIVKGKNGYSRSHYKNSSSLSHFEHLIER 953
L DG T ++N+LVY P+ + ++ NG S +NS ++ E+ ER
Sbjct: 851 ILHTKDGLFCTCVVQNALVYTPHNGYVYCTKGVLNNLNGNSLLTKRNSGDQTYIEYYEER 910
Query: 954 FGIHLKYPEQPLLSAKPLFHLHNLLHDRRQGNSEACYLQEYFIDLPPELCQLKIIGFSKD 1013
GI L + ++PLL+ + +F LH+ LH ++ + F++LPPELC + + S D
Sbjct: 911 HGIQLNFVDEPLLNGRHIFTLHSYLHMAKKKKEKE--HDREFVELPPELCHVILSPISVD 970
Query: 1014 IGSSISLLPSIMHRLENLLVAIELKCMLAAAFPDGAEVTANRILEALTTEKCQERISLER 1073
+ S + +PS+M R+E+LL+A LK + + ++LEA+TT+KC+++ LE
Sbjct: 971 MIYSYTFIPSVMQRIESLLIAYNLKKSIPK-----VNIPTIKVLEAITTKKCEDQFHLES 1030
Query: 1074 LEILGDGFLKFAVARYLFLTHDKSDEGELTRRRSYLVKNINLFKLATRKNLQVYIRDQPF 1133
LE LGD FLK+AV + LF EG L+ ++ ++ N+ L + ++ LQ +IRD+ F
Sbjct: 1031 LETLGDSFLKYAVCQQLFQHCHTHHEGLLSTKKDGMISNVMLCQFGCQQKLQGFIRDECF 1090
Query: 1134 EPSQFYTLGRPCP--RICNEETREDIHSHCNATKNAKASETKCSRGHHWLHKKTISDVVE 1193
EP + G+ + N+ E + + + +N K +K+++DVVE
Sbjct: 1091 EPKGWMVPGQSSAAYSLVNDTLPESRNIYVASRRNLK--------------RKSVADVVE 1150
Query: 1194 ALVGAFLVDSGFKAAISFLKWIGIQVEFEASLVTNALMSSNAYIILADRINISVLQNSLG 1253
+L+GA+L + G AA+ F+ W+GI+V+F T + ++ I +N+ +++ L
Sbjct: 1151 SLIGAYLSEGGELAALMFMNWVGIKVDF-----TTTKIQRDSPIQAEKLVNVGYMESLLN 1210
Query: 1254 HQFLHKGLLLQALVHPSY-HKHGGGCYQRLEFLGDAVLDYLITSYLYSAYPKLKPGLLTD 1313
+ F K LL++AL H SY CYQRLEFLGD+VLDYLIT +LY YP L PGLLTD
Sbjct: 1211 YSFEDKSLLVEALTHGSYMMPEIPRCYQRLEFLGDSVLDYLITKHLYDKYPCLSPGLLTD 1270
Query: 1314 LRSVFVRNEAFANVAVDRSFYKYLICDSTSLQSDIKSYVNFIKAPS--SGSGSLGLPSCP 1373
+RS V NE +A VAV + +K+++ S L I V+ + S S G S P
Sbjct: 1271 MRSASVNNECYALVAVKANLHKHILYASHHLHKHISRTVSEFEQSSLQSTFGWESDISFP 1330
Query: 1374 KALGDLVESCVGAVLVDTGFDMNYVWKIMLSFLDPIISFSGFQLSPIRDIIEFCQDCGSN 1433
K LGD++ES GA+ VD+G++ V+ + L +I+ +L P+R++ E CQ
Sbjct: 1331 KVLGDVIESLAGAIFVDSGYNKEVVFASIKPLLGCMITPETVKLHPVRELTELCQ----K 1369
Query: 1434 LQFNESKTGKYYSVEAEVKGDNFHATASAAHRRKRDAKKIAANLILTKLK 1462
QF SK + S EVK +A K+ AKK+A +L LK
Sbjct: 1391 WQFELSKAKDFDSFTVEVKAKEMSFAHTAKASDKKMAKKLAYKEVLNLLK 1369
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
P84634 | 0.0e+00 | 54.00 | Dicer-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=DCL4 PE=1 SV=2 | [more] |
A7LFZ6 | 0.0e+00 | 48.25 | Endoribonuclease Dicer homolog 4 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL... | [more] |
Q5N870 | 1.0e-203 | 31.23 | Endoribonuclease Dicer homolog 3a OS=Oryza sativa subsp. japonica OX=39947 GN=DC... | [more] |
Q3EBC8 | 2.1e-188 | 32.89 | Endoribonuclease Dicer homolog 2 OS=Arabidopsis thaliana OX=3702 GN=At3g03300 PE... | [more] |
Q7XD96 | 1.4e-181 | 31.37 | Endoribonuclease Dicer homolog 3b OS=Oryza sativa subsp. japonica OX=39947 GN=DC... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1C050 | 0.0e+00 | 98.19 | dicer-like protein 4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007071 P... | [more] |
A0A6J1BZQ8 | 0.0e+00 | 98.58 | dicer-like protein 4 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007071 P... | [more] |
A0A6J1JAJ5 | 0.0e+00 | 82.10 | dicer-like protein 4 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485046 PE=3... | [more] |
A0A6J1E1P4 | 0.0e+00 | 82.36 | dicer-like protein 4 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429985 PE... | [more] |
A0A6J1JJM5 | 0.0e+00 | 82.04 | dicer-like protein 4 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485046 PE=3... | [more] |