MS002337 (gene) Bitter gourd (TR) v1

Overview


Name	MS002337
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Eukaryotic translation initiation factor 5A
Location	scaffold30: 4206656 .. 4208683 (-)
RNA-Seq Expression	MS002337
Synteny	MS002337

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCGACGAGGAGCACCACTTCGACTCCAAGGCCGACGCCGGAGCGTCCAAGACTTTCCCTCAGCAAGCCGGAACCATTCGCAAGAATGGCTACATCGTAATCAAGAACAGGCCCTGCAAGGTTTCTTTCATTTTTCCCCATTCCACTTTGGACTCTGAAGTTGATCAAAATTCTTGGGGTTTTTTTAATTTGTTGAAACATGCAAGTGGGATTGGATTGGATTGTGCTATTTTATTCACTACTCTTTAACTTCTCGTCCAGATTCTTTATTTTAATTTTTGCTTGGCCCTTTGTTTTCAGAGAGGATAGACATTCGATTTCTTCCCTTGTTTCGATAATGATATTTCTAAATGAAGTATGTCGCTAACTTCTAACTCGGTGATTACTGATTTCGGTAATTGTCACTGTTAGGTTATATATTTAGTTTTTCCCCTTTAGATTTTCTTCGTGGGATCTTGTGCTTTTTAGGTTTTTAAGAATTGCTATGGATTTGGATGGTTTTTGCACAACTATTGTGGATGGAAACTCTGTATTGTCTTGGATGCTCAGCCTTTCAAGCTGATCTTGGTTACACGAGAAAGACTAGAATCATTTAGTTACCTAAAAATCAGGCGGTAAACCTATGATAACAATTGCAGGAGAAATGAACTTTGAAGAAGTTGTTCAAGATTTGACATAGTGGGTTTGGTGTAAGTGGAAGCTTGTGAAACTCGAGTGTTACTGGTTGATTATTTACTTATCTTGAATGCCAAGTGATTCTTTCACCCACGTTTGAAGTGTAATAAGAAATATGTGGCCTTGGTCTGTCATAAGAGTAGATCATGATTATGTTGTCTTTAGTCAGTTTTATTAGATGGACTTGGTTTATGGACTCGTTGAGTTCGTAAGTCCTGTTATTGGACACACCATTAAATATTTCAATGAGATCTCGCTGCAATTTCCTGCATACCAATACCATCAAATGTCCCAGCTGTTTCTTGTAGGACAAGCAGCGGATAGATGGCGGGGCGGTAAGGAGCGGATATAGGCCACATGTTGTAGTTTTTTTCGCAATGTCTCTCTTTTGAAATATGCTATGCACGCCGCCTTTTTGTGTCAAATATCAATATACATTATACAATGTCCAACGTTATAGTCCATTTGCCATGATAATGTGTAGTTGAAGTTTTACTCTTCAATACGGAGGCAGTCCATTGACTTGAAGGTTTTTTGAAGTAAGCTGTGCTTATATGGGATGGGATGCTTTCAAGCTACCTACCAAGCATATCATGTTGCAGTATATCACTGTTTTATTATTCCCATTAACCTCCTTTTCAGGTTGTTGAAGTTTCCACTTCAAAAACTGGAAAGCATGGACATGCAAAGTGTCACTTTGTGGCCATCGACATATTCAATGGGAAGAAACTTGAAGATATCGTTCCTTCTTCTCACAACTGTGATGTAATTTCTTTGTCATCCTCTTCTTGTGTTCTACTTTCTAAGTGGTGGTTTTATTGTTCTATGCAACTTTGCTTGTTAGTCCTCAATCCTCATATTACCAATGCTTCTGCAGGTTCCCCATGTTAATCGTACTGACTATCAGCTGATCGATATATCTGAGGATGGTTTTGTAAGTTCTATTATAATCTCCCTGTTCAAGACCTCCCTCTCAAAGAAAAAAAGGAAAAAAAACACACGTTCGTAAAGACTGAACAAACAGAAGAAATGAAGGTAAGATGGAGTCTTGACCAATTAACTTGTTATTTTGCAGGTCAGTCTCCTTACTGAAAATGGAAACACTAAGGATGATTTGAAGCTTCCCACCGATGATAATCTGCTGACGCAGGTATACAACGATGACATCACTATATTTACAAACCTTCAAGTCATTACCATGTAGTTTTCTGCAACTTCATAAATTTTCATTTATTTTCATGTCGATAGCTTAAAGATGGGTTTGCTGAAGGAAAAGATCTCGTCCTAACTGTCATGTCTGCAATGGGAGAAGAGCAGATCTGTGCTCTCAAGGACATCGGTCCTAAAAAC

mRNA sequence

ATGTCCGACGAGGAGCACCACTTCGACTCCAAGGCCGACGCCGGAGCGTCCAAGACTTTCCCTCAGCAAGCCGGAACCATTCGCAAGAATGGCTACATCGTAATCAAGAACAGGCCCTGCAAGGTTGTTGAAGTTTCCACTTCAAAAACTGGAAAGCATGGACATGCAAAGTGTCACTTTGTGGCCATCGACATATTCAATGGGAAGAAACTTGAAGATATCGTTCCTTCTTCTCACAACTGTGATGTTCCCCATGTTAATCGTACTGACTATCAGCTGATCGATATATCTGAGGATGGTTTTGTCAGTCTCCTTACTGAAAATGGAAACACTAAGGATGATTTGAAGCTTCCCACCGATGATAATCTGCTGACGCAGCTTAAAGATGGGTTTGCTGAAGGAAAAGATCTCGTCCTAACTGTCATGTCTGCAATGGGAGAAGAGCAGATCTGTGCTCTCAAGGACATCGGTCCTAAAAAC

Coding sequence (CDS)

ATGTCCGACGAGGAGCACCACTTCGACTCCAAGGCCGACGCCGGAGCGTCCAAGACTTTCCCTCAGCAAGCCGGAACCATTCGCAAGAATGGCTACATCGTAATCAAGAACAGGCCCTGCAAGGTTGTTGAAGTTTCCACTTCAAAAACTGGAAAGCATGGACATGCAAAGTGTCACTTTGTGGCCATCGACATATTCAATGGGAAGAAACTTGAAGATATCGTTCCTTCTTCTCACAACTGTGATGTTCCCCATGTTAATCGTACTGACTATCAGCTGATCGATATATCTGAGGATGGTTTTGTCAGTCTCCTTACTGAAAATGGAAACACTAAGGATGATTTGAAGCTTCCCACCGATGATAATCTGCTGACGCAGCTTAAAGATGGGTTTGCTGAAGGAAAAGATCTCGTCCTAACTGTCATGTCTGCAATGGGAGAAGAGCAGATCTGTGCTCTCAAGGACATCGGTCCTAAAAAC

Protein sequence

MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTDDNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPKN

Homology

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BLAST of MS002337 vs. NCBI nr
Match: XP_022135162.1 (eukaryotic translation initiation factor 5A-2-like [Momordica charantia])

HSP 1 Score: 330.5 bits (846), Expect = 8.1e-87
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD 120
           VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD
Sbjct: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD 120

Query: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPKN 161
           DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPKN
Sbjct: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPKN 160

[Close]

BLAST of MS002337 vs. NCBI nr
Match: XP_008443857.1 (PREDICTED: eukaryotic translation initiation factor 5A-2 [Cucumis melo] >XP_038880020.1 eukaryotic translation initiation factor 5A-2 [Benincasa hispida] >KAA0035077.1 eukaryotic translation initiation factor 5A-2 [Cucumis melo var. makuwa] >TYK03602.1 eukaryotic translation initiation factor 5A-2 [Cucumis melo var. makuwa])

HSP 1 Score: 327.4 bits (838), Expect = 6.9e-86
Identity = 158/160 (98.75%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD 120
           VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDL+LPTD
Sbjct: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTD 120

Query: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPKN 161
           DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPK+
Sbjct: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPKS 160

[Close]

BLAST of MS002337 vs. NCBI nr
Match: XP_004146682.1 (eukaryotic translation initiation factor 5A-2 [Cucumis sativus] >KGN65160.1 hypothetical protein Csa_019565 [Cucumis sativus])

HSP 1 Score: 327.4 bits (838), Expect = 6.9e-86
Identity = 158/160 (98.75%), Postives = 159/160 (99.38%), Query Frame = 0


Query: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFDSKADAGASKTFPQQAG IRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFDSKADAGASKTFPQQAGAIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD 120
           VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDL+LPTD
Sbjct: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTD 120

Query: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPKN 161
           DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPKN
Sbjct: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPKN 160

[Close]

BLAST of MS002337 vs. NCBI nr
Match: XP_022930431.1 (eukaryotic translation initiation factor 5A-2-like [Cucurbita moschata] >XP_022969168.1 eukaryotic translation initiation factor 5A-2-like [Cucurbita maxima] >XP_023529298.1 eukaryotic translation initiation factor 5A-2-like [Cucurbita pepo subsp. pepo] >KAG6587824.1 Eukaryotic translation initiation factor 5A-2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035679.1 Eukaryotic translation initiation factor 5A-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 326.6 bits (836), Expect = 1.2e-85
Identity = 158/159 (99.37%), Postives = 159/159 (100.00%), Query Frame = 0


Query: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD 120
           VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDL+LPTD
Sbjct: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTD 120

Query: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPK 160
           DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPK
Sbjct: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPK 159

[Close]

BLAST of MS002337 vs. NCBI nr
Match: KAF9662748.1 (hypothetical protein SADUNF_Sadunf18G0086500 [Salix dunnii])

HSP 1 Score: 322.8 bits (826), Expect = 1.7e-84
Identity = 154/160 (96.25%), Postives = 158/160 (98.75%), Query Frame = 0


Query: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHF+SKADAGASKT+PQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD 120
           V IDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD
Sbjct: 61  VGIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD 120

Query: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPKN 161
           DNLLTQ+KDGF EGKDLV+TVMSAMGEEQICALKDIGPKN
Sbjct: 121 DNLLTQIKDGFGEGKDLVVTVMSAMGEEQICALKDIGPKN 160

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BLAST of MS002337 vs. ExPASy Swiss-Prot
Match: Q9AXJ4 (Eukaryotic translation initiation factor 5A OS=Manihot esculenta OX=3983 PE=2 SV=2)

HSP 1 Score: 313.5 bits (802), Expect = 1.3e-84
Identity = 148/160 (92.50%), Postives = 158/160 (98.75%), Query Frame = 0


Query: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHF+SKADAGASKTFPQQAGTIRKNGYIVIKNRPCKV+EVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVMEVSTSKTGKHGHAKCHF 60

Query: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD 120
           V IDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTE GNTKDDL+LPTD
Sbjct: 61  VGIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTETGNTKDDLRLPTD 120

Query: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPKN 161
           +NLL+Q+KDGFAEGKDLV++VMSAMGEE+IC+LKDIGPKN
Sbjct: 121 ENLLSQIKDGFAEGKDLVVSVMSAMGEERICSLKDIGPKN 160

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BLAST of MS002337 vs. ExPASy Swiss-Prot
Match: P24922 (Eukaryotic translation initiation factor 5A-2 OS=Nicotiana plumbaginifolia OX=4092 GN=EIF-5A2 PE=2 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 8.7e-84
Identity = 149/159 (93.71%), Postives = 156/159 (98.11%), Query Frame = 0


Query: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEH F+SKADAGASKT+PQQAGTIRKNG+IVIK RPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHQFESKADAGASKTYPQQAGTIRKNGHIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD 120
           VAIDIF GKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDL+LPTD
Sbjct: 61  VAIDIFTGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTD 120

Query: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPK 160
           DNLLTQ+KDGFAEGKDLV++VMSAMGEEQICALKDIGPK
Sbjct: 121 DNLLTQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGPK 159

[Close]

BLAST of MS002337 vs. ExPASy Swiss-Prot
Match: P69039 (Eukaryotic translation initiation factor 5A-1 OS=Nicotiana plumbaginifolia OX=4092 GN=EIF-5A1 PE=2 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 1.5e-83
Identity = 147/159 (92.45%), Postives = 155/159 (97.48%), Query Frame = 0


Query: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHF+SKADAGASKT+PQQAGTIRKNG+IVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGHIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD 120
           VAIDIF GKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDL+LPTD
Sbjct: 61  VAIDIFTGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTD 120

Query: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPK 160
           DNLL  +KDGFAEGKDLVL+VMSAMGEEQIC +KD+GPK
Sbjct: 121 DNLLALIKDGFAEGKDLVLSVMSAMGEEQICGIKDVGPK 159

[Close]

BLAST of MS002337 vs. ExPASy Swiss-Prot
Match: P69040 (Eukaryotic translation initiation factor 5A-1 OS=Nicotiana tabacum OX=4097 GN=EIF-5A1 PE=3 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 1.5e-83
Identity = 147/159 (92.45%), Postives = 155/159 (97.48%), Query Frame = 0


Query: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHF+SKADAGASKT+PQQAGTIRKNG+IVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFESKADAGASKTYPQQAGTIRKNGHIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD 120
           VAIDIF GKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDL+LPTD
Sbjct: 61  VAIDIFTGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTD 120

Query: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPK 160
           DNLL  +KDGFAEGKDLVL+VMSAMGEEQIC +KD+GPK
Sbjct: 121 DNLLALIKDGFAEGKDLVLSVMSAMGEEQICGIKDVGPK 159

[Close]

BLAST of MS002337 vs. ExPASy Swiss-Prot
Match: Q945F4 (Eukaryotic translation initiation factor 5A-2 OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 2.5e-83
Identity = 147/159 (92.45%), Postives = 157/159 (98.74%), Query Frame = 0


Query: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHF+  ADAGASKT+PQQAGTIRKNGYIVIK+RPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFEPAADAGASKTYPQQAGTIRKNGYIVIKSRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD 120
           VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLT+NG+TKDDLKLPTD
Sbjct: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTD 120

Query: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPK 160
           D+LLTQ+KDGFA+GKDLV++VMSAMGEEQICALKDIGPK
Sbjct: 121 DSLLTQIKDGFADGKDLVVSVMSAMGEEQICALKDIGPK 159

[Close]

BLAST of MS002337 vs. ExPASy TrEMBL
Match: A0A6J1C1W6 (Eukaryotic translation initiation factor 5A OS=Momordica charantia OX=3673 GN=LOC111007197 PE=3 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 3.9e-87
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD 120
           VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD
Sbjct: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD 120

Query: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPKN 161
           DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPKN
Sbjct: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPKN 160

[Close]

BLAST of MS002337 vs. ExPASy TrEMBL
Match: A0A5A7T0H1 (Eukaryotic translation initiation factor 5A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1369G00200 PE=3 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 3.3e-86
Identity = 158/160 (98.75%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD 120
           VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDL+LPTD
Sbjct: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTD 120

Query: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPKN 161
           DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPK+
Sbjct: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPKS 160

[Close]

BLAST of MS002337 vs. ExPASy TrEMBL
Match: A0A0A0LTB3 (Eukaryotic translation initiation factor 5A OS=Cucumis sativus OX=3659 GN=Csa_1G256200 PE=3 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 3.3e-86
Identity = 158/160 (98.75%), Postives = 159/160 (99.38%), Query Frame = 0


Query: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFDSKADAGASKTFPQQAG IRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFDSKADAGASKTFPQQAGAIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD 120
           VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDL+LPTD
Sbjct: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTD 120

Query: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPKN 161
           DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPKN
Sbjct: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPKN 160

[Close]

BLAST of MS002337 vs. ExPASy TrEMBL
Match: A0A1S3B9T8 (Eukaryotic translation initiation factor 5A OS=Cucumis melo OX=3656 GN=LOC103487352 PE=3 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 3.3e-86
Identity = 158/160 (98.75%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD 120
           VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDL+LPTD
Sbjct: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTD 120

Query: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPKN 161
           DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPK+
Sbjct: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPKS 160

[Close]

BLAST of MS002337 vs. ExPASy TrEMBL
Match: A0A6J1I076 (Eukaryotic translation initiation factor 5A OS=Cucurbita maxima OX=3661 GN=LOC111468243 PE=3 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 5.7e-86
Identity = 158/159 (99.37%), Postives = 159/159 (100.00%), Query Frame = 0


Query: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD 120
           VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDL+LPTD
Sbjct: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLRLPTD 120

Query: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPK 160
           DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPK
Sbjct: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPK 159

[Close]

BLAST of MS002337 vs. TAIR 10
Match: AT1G13950.1 (eukaryotic elongation factor 5A-1 )

HSP 1 Score: 288.5 bits (737), Expect = 3.3e-78
Identity = 140/159 (88.05%), Postives = 150/159 (94.34%), Query Frame = 0


Query: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSDEEHHF+S +DAGASKT+PQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDEEHHFES-SDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD 120
           VAIDIF  KKLEDIVPSSHNCDVPHVNRTDYQLIDISEDG+VSLLT+NG+TKDDLKLP D
Sbjct: 61  VAIDIFTSKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPND 120

Query: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPK 160
           D LL Q+K GF +GKDLV++VMSAMGEEQI ALKDIGPK
Sbjct: 121 DTLLQQIKSGFDDGKDLVVSVMSAMGEEQINALKDIGPK 158

[Close]

BLAST of MS002337 vs. TAIR 10
Match: AT1G69410.1 (eukaryotic elongation factor 5A-3 )

HSP 1 Score: 283.5 bits (724), Expect = 1.1e-76
Identity = 135/159 (84.91%), Postives = 150/159 (94.34%), Query Frame = 0


Query: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSD+EHHF+S +DAGASKT+PQQAG IRK G+IVIK RPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDDEHHFES-SDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD 120
           VAIDIF  KKLEDIVPSSHNCDVPHVNR DYQLIDISEDGFVSLLT+NG+TKDDLKLPTD
Sbjct: 61  VAIDIFTSKKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTD 120

Query: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIGPK 160
           + LLTQLK+GF EGKD+V++VMSAMGEEQ+CALK++GPK
Sbjct: 121 EALLTQLKNGFEEGKDIVVSVMSAMGEEQMCALKEVGPK 158

[Close]

BLAST of MS002337 vs. TAIR 10
Match: AT1G26630.1 (Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) protein )

HSP 1 Score: 264.6 bits (675), Expect = 5.1e-71
Identity = 124/157 (78.98%), Postives = 144/157 (91.72%), Query Frame = 0


Query: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSD+EHHF++ +++GASKT+PQ AG IRK G+IVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDDEHHFEA-SESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD 120
           VAIDIF  KKLEDIVPSSHNCDVPHVNR DYQLIDI+EDGFVSLLT++G TKDDLKLPTD
Sbjct: 61  VAIDIFTAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTD 120

Query: 121 DNLLTQLKDGFAEGKDLVLTVMSAMGEEQICALKDIG 158
           D L  Q++ GF EGKD+V++VMS+MGEEQICA+K++G
Sbjct: 121 DGLTAQMRLGFDEGKDIVVSVMSSMGEEQICAVKEVG 156

[Close]

BLAST of MS002337 vs. TAIR 10
Match: AT1G26630.2 (Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) protein )

HSP 1 Score: 221.9 bits (564), Expect = 3.8e-58
Identity = 105/127 (82.68%), Postives = 116/127 (91.34%), Query Frame = 0


Query: 1   MSDEEHHFDSKADAGASKTFPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60
           MSD+EHHF++ +++GASKT+PQ AG IRK G+IVIKNRPCKVVEVSTSKTGKHGHAKCHF
Sbjct: 1   MSDDEHHFEA-SESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHF 60

Query: 61  VAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTD 120
           VAIDIF  KKLEDIVPSSHNCDVPHVNR DYQLIDI+EDGFVSLLT++G TKDDLKLPTD
Sbjct: 61  VAIDIFTAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTD 120

Query: 121 DNLLTQL 128
           D L  QL
Sbjct: 121 DGLTAQL 126

The following BLAST results are available for this feature:

BLAST of MS002337 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135162.1	8.1e-87	100.00	eukaryotic translation initiation factor 5A-2-like [Momordica charantia]	[more]
XP_008443857.1	6.9e-86	98.75	PREDICTED: eukaryotic translation initiation factor 5A-2 [Cucumis melo] >XP_0388...	[more]
XP_004146682.1	6.9e-86	98.75	eukaryotic translation initiation factor 5A-2 [Cucumis sativus] >KGN65160.1 hypo...	[more]
XP_022930431.1	1.2e-85	99.37	eukaryotic translation initiation factor 5A-2-like [Cucurbita moschata] >XP_0229...	[more]
KAF9662748.1	1.7e-84	96.25	hypothetical protein SADUNF_Sadunf18G0086500 [Salix dunnii]	[more]

BLAST of MS002337 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9AXJ4	1.3e-84	92.50	Eukaryotic translation initiation factor 5A OS=Manihot esculenta OX=3983 PE=2 SV...	[more]
P24922	8.7e-84	93.71	Eukaryotic translation initiation factor 5A-2 OS=Nicotiana plumbaginifolia OX=40...	[more]
P69039	1.5e-83	92.45	Eukaryotic translation initiation factor 5A-1 OS=Nicotiana plumbaginifolia OX=40...	[more]
P69040	1.5e-83	92.45	Eukaryotic translation initiation factor 5A-1 OS=Nicotiana tabacum OX=4097 GN=EI...	[more]
Q945F4	2.5e-83	92.45	Eukaryotic translation initiation factor 5A-2 OS=Medicago sativa OX=3879 PE=2 SV...	[more]

BLAST of MS002337 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C1W6	3.9e-87	100.00	Eukaryotic translation initiation factor 5A OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A5A7T0H1	3.3e-86	98.75	Eukaryotic translation initiation factor 5A OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A0A0LTB3	3.3e-86	98.75	Eukaryotic translation initiation factor 5A OS=Cucumis sativus OX=3659 GN=Csa_1G...	[more]
A0A1S3B9T8	3.3e-86	98.75	Eukaryotic translation initiation factor 5A OS=Cucumis melo OX=3656 GN=LOC103487...	[more]
A0A6J1I076	5.7e-86	99.37	Eukaryotic translation initiation factor 5A OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]

BLAST of MS002337 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G13950.1	3.3e-78	88.05	eukaryotic elongation factor 5A-1	[more]
AT1G69410.1	1.1e-76	84.91	eukaryotic elongation factor 5A-3	[more]
AT1G26630.1	5.1e-71	78.98	Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) protein	[more]
AT1G26630.2	3.8e-58	82.68	Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020189	Translation elongation factor, IF5A C-terminal	SMART	SM01376	eIF_5a_2	coord: 85..154 e-value: 6.1E-34 score: 128.8
IPR020189	Translation elongation factor, IF5A C-terminal	PFAM	PF01287	eIF-5a	coord: 85..154 e-value: 1.2E-28 score: 98.9
IPR001884	Translation elongation factor IF5A-like	PIRSF	PIRSF003025	Transl_init_eIF5A	coord: 1..157 e-value: 6.7E-67 score: 221.8
IPR001884	Translation elongation factor IF5A-like	TIGRFAM	TIGR00037	TIGR00037	coord: 18..153 e-value: 1.9E-41 score: 138.9
IPR001884	Translation elongation factor IF5A-like	PANTHER	PTHR11673	TRANSLATION INITIATION FACTOR 5A FAMILY MEMBER	coord: 1..156
None	No IPR available	GENE3D	2.40.50.140		coord: 86..159 e-value: 2.8E-34 score: 119.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..21
None	No IPR available	PANTHER	PTHR11673:SF42	BNAA07G09420D PROTEIN	coord: 1..156
None	No IPR available	CDD	cd04468	S1_eIF5A	coord: 86..156 e-value: 4.38775E-31 score: 104.927
IPR014722	Ribosomal protein L2, domain 2	GENE3D	2.30.30.30		coord: 1..85 e-value: 5.6E-43 score: 146.7
IPR019769	Translation elongation factor, IF5A, hypusine site	PROSITE	PS00302	IF5A_HYPUSINE	coord: 50..57
IPR008991	Translation protein SH3-like domain superfamily	SUPERFAMILY	50104	Translation proteins SH3-like domain	coord: 15..93
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 86..156

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS002337.1	MS002337.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045901	positive regulation of translational elongation
biological_process	GO:0045905	positive regulation of translational termination
molecular_function	GO:0043022	ribosome binding
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0003746	translation elongation factor activity