MS002323 (gene) Bitter gourd (TR) v1

Overview
NameMS002323
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionE3 ubiquitin-protein ligase MIEL1-like
Locationscaffold30: 4150853 .. 4151164 (+)
RNA-Seq ExpressionMS002323
SyntenyMS002323
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCAGTTAAACCCTTCGTTTCGATAGCCCTGATTGCTCTTCTCTTGTTTTCTGAATCCCTCGCTGTTTCGTCCGCTGACTACGGCCCGGATTCGAATTCCTTCACCGGAATACTCCGCCGGAATCTGCTCCACCCATCGGGGCGTCGGCGGGCAGATAATAGCGTCGGCTCCAATTTGGCCGAGAAGATCAGTATCAAAAGGGGGGTTCGATCCGCCGTAATTGCTGGTCGTGCCAGTGGATCGCCGTCCAGCCGGGCACCGCCCTTTCATTTTGGGTCTCTGCTCTGTTTCTGCATCGCCTTTGCG

mRNA sequence

ATGGCTGCAGTTAAACCCTTCGTTTCGATAGCCCTGATTGCTCTTCTCTTGTTTTCTGAATCCCTCGCTGTTTCGTCCGCTGACTACGGCCCGGATTCGAATTCCTTCACCGGAATACTCCGCCGGAATCTGCTCCACCCATCGGGGCGTCGGCGGGCAGATAATAGCGTCGGCTCCAATTTGGCCGAGAAGATCAGTATCAAAAGGGGGGTTCGATCCGCCGTAATTGCTGGTCGTGCCAGTGGATCGCCGTCCAGCCGGGCACCGCCCTTTCATTTTGGGTCTCTGCTCTGTTTCTGCATCGCCTTTGCG

Coding sequence (CDS)

ATGGCTGCAGTTAAACCCTTCGTTTCGATAGCCCTGATTGCTCTTCTCTTGTTTTCTGAATCCCTCGCTGTTTCGTCCGCTGACTACGGCCCGGATTCGAATTCCTTCACCGGAATACTCCGCCGGAATCTGCTCCACCCATCGGGGCGTCGGCGGGCAGATAATAGCGTCGGCTCCAATTTGGCCGAGAAGATCAGTATCAAAAGGGGGGTTCGATCCGCCGTAATTGCTGGTCGTGCCAGTGGATCGCCGTCCAGCCGGGCACCGCCCTTTCATTTTGGGTCTCTGCTCTGTTTCTGCATCGCCTTTGCG

Protein sequence

MAAVKPFVSIALIALLLFSESLAVSSADYGPDSNSFTGILRRNLLHPSGRRRADNSVGSNLAEKISIKRGVRSAVIAGRASGSPSSRAPPFHFGSLLCFCIAFA
Homology
BLAST of MS002323 vs. NCBI nr
Match: KAG6587814.1 (hypothetical protein SDJN03_16379, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 113.6 bits (283), Expect = 1.0e-21
Identity = 63/104 (60.58%), Postives = 71/104 (68.27%), Query Frame = 0

Query: 1   MAAVKPFVSIALIALLLFSESLAVSSADYGPDSNSFTGILRRNLLHPSGRRRADNSVGSN 60
           MA +K    I LI LLL SES  VSSADY  D  S TGIL RN+LHP   +R D   GS+
Sbjct: 1   MAPIKRLFPITLICLLLLSESFPVSSADYDQDWKSLTGILHRNVLHPLEHQRLDKDTGSS 60

Query: 61  LAEKISIKRGVRSAVIAGRASGSPSSRAPPFHFGSLLCFCIAFA 105
           LAEKISIKRG RSAVI G +S S SSR PPF+  S++CF  AFA
Sbjct: 61  LAEKISIKRGARSAVIGGHSSISSSSRVPPFNIASVICFVFAFA 104

BLAST of MS002323 vs. NCBI nr
Match: KAG6575859.1 (hypothetical protein SDJN03_26498, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014393.1 hypothetical protein SDJN02_24570, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 76.3 bits (186), Expect = 1.8e-10
Identity = 48/99 (48.48%), Postives = 62/99 (62.63%), Query Frame = 0

Query: 1  MAAVKPFVSIALIALLLFSESLAVSSADYGPDSNSFTGILRRNLLHPSGRRRADNSVGSN 60
          MAA+K  VS+AL+ALLLFSESL  S+ D   DSNS TG+LRRN+L  +  + ADNSV S+
Sbjct: 1  MAALKSLVSVALLALLLFSESLTASAVDRSGDSNSLTGMLRRNVLQTAAHQIADNSVESS 60

Query: 61 LAEK--ISIKRGVRSAVIAGRASGSPSSRAPPFHFGSLL 98
             K  + IKR +R      R+S +  SR      GS+L
Sbjct: 61 PERKMNVHIKRRMRPVATGTRSSAANRSRIASIGVGSVL 99

BLAST of MS002323 vs. NCBI nr
Match: KGN65176.1 (hypothetical protein Csa_019975 [Cucumis sativus])

HSP 1 Score: 70.9 bits (172), Expect = 7.5e-09
Identity = 53/107 (49.53%), Postives = 67/107 (62.62%), Query Frame = 0

Query: 2   AAVKPF-VSIALIALLLFSESL-AVSSADYGPDSNSFTGILRRNLLHPSGRRRADNSVGS 61
           AA+  F + IALI+LLLFSESL A+SS+D+  DSN                R  D   G+
Sbjct: 3   AAIHRFLLPIALISLLLFSESLPAISSSDHIQDSN----------------RPGDKDSGT 62

Query: 62  NLAEKISIKRGVRSAVIAGRASGSPSSRAPP---FHFGSLLCFCIAF 104
           +L EKISIKRGVRS VIAGR++ S S+R      F+ GS++CF IAF
Sbjct: 63  SLTEKISIKRGVRSGVIAGRSTTSSSARVSSSSFFNVGSVICFNIAF 93

BLAST of MS002323 vs. NCBI nr
Match: KAA0035100.1 (hypothetical protein E6C27_scaffold57G001750 [Cucumis melo var. makuwa] >TYJ95978.1 hypothetical protein E5676_scaffold271G00480 [Cucumis melo var. makuwa])

HSP 1 Score: 65.9 bits (159), Expect = 2.4e-07
Identity = 50/107 (46.73%), Postives = 65/107 (60.75%), Query Frame = 0

Query: 1   MAAVKPF-VSIALIALLLFSESL-AVSSADYGPDSNSFTGILRRNLLHPSGRRRADNSVG 60
           MAA+  F + +ALI+LLLFSESL A+SS D+G +SN                RR D    
Sbjct: 1   MAAIHRFLLPLALISLLLFSESLAAISSTDHGQNSN----------------RRGDKGSD 60

Query: 61  SNLAEKISIKRGVRSAVIAGRASGSPSSRAPP--FHFGSLLCFCIAF 104
           ++LA KI  KRGVRS V AGR++ S S++     F+ GSL+CF IAF
Sbjct: 61  TSLAGKIISKRGVRSGVTAGRSTSSSSAQMSSSFFNVGSLICFNIAF 91

BLAST of MS002323 vs. NCBI nr
Match: KAG6575858.1 (hypothetical protein SDJN03_26497, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 64.7 bits (156), Expect = 5.4e-07
Identity = 46/106 (43.40%), Postives = 62/106 (58.49%), Query Frame = 0

Query: 1   MAAVKPFVSIALIALLLFSESLAVSSADYGPDSNSFTGILRRNLLHPSGRRR-ADNSVGS 60
           MA +K  +SIAL+ALLL SE L +S+AD+G   +   G+ RR+ L  + R + A+N +G 
Sbjct: 1   MAPIKSLISIALLALLLLSEHLPISAADHGVAPSPLAGVFRRDTLQTAERHQVAENGIGY 60

Query: 61  NLAEKISIKRGVRSAVIAGRASG-SPS----SRAPPFHFGSLLCFC 101
             + +  I+R  R AV   RA G SPS    SR   F  GSLLC C
Sbjct: 61  QYSFEQKIRRRARRAV--RRAEGPSPSSANWSRTSSFGIGSLLCLC 104

BLAST of MS002323 vs. ExPASy TrEMBL
Match: A0A0A0LYV9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G257330 PE=4 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 3.6e-09
Identity = 53/107 (49.53%), Postives = 67/107 (62.62%), Query Frame = 0

Query: 2   AAVKPF-VSIALIALLLFSESL-AVSSADYGPDSNSFTGILRRNLLHPSGRRRADNSVGS 61
           AA+  F + IALI+LLLFSESL A+SS+D+  DSN                R  D   G+
Sbjct: 3   AAIHRFLLPIALISLLLFSESLPAISSSDHIQDSN----------------RPGDKDSGT 62

Query: 62  NLAEKISIKRGVRSAVIAGRASGSPSSRAPP---FHFGSLLCFCIAF 104
           +L EKISIKRGVRS VIAGR++ S S+R      F+ GS++CF IAF
Sbjct: 63  SLTEKISIKRGVRSGVIAGRSTTSSSARVSSSSFFNVGSVICFNIAF 93

BLAST of MS002323 vs. ExPASy TrEMBL
Match: A0A5D3BCG4 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold271G00480 PE=4 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.2e-07
Identity = 50/107 (46.73%), Postives = 65/107 (60.75%), Query Frame = 0

Query: 1   MAAVKPF-VSIALIALLLFSESL-AVSSADYGPDSNSFTGILRRNLLHPSGRRRADNSVG 60
           MAA+  F + +ALI+LLLFSESL A+SS D+G +SN                RR D    
Sbjct: 1   MAAIHRFLLPLALISLLLFSESLAAISSTDHGQNSN----------------RRGDKGSD 60

Query: 61  SNLAEKISIKRGVRSAVIAGRASGSPSSRAPP--FHFGSLLCFCIAF 104
           ++LA KI  KRGVRS V AGR++ S S++     F+ GSL+CF IAF
Sbjct: 61  TSLAGKIISKRGVRSGVTAGRSTSSSSAQMSSSFFNVGSLICFNIAF 91

BLAST of MS002323 vs. ExPASy TrEMBL
Match: A0A6J1JZB1 (uncharacterized protein LOC111488793 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488793 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.3e-06
Identity = 45/109 (41.28%), Postives = 62/109 (56.88%), Query Frame = 0

Query: 1   MAAVKPFVSIALIALLLFSESLAVSSADYGPDSNSFTGILRRNLLHPSGRRR-ADNSVGS 60
           MA +K  +SIAL+ALLL  E L +S+AD+G   +   G+ R + L  + R + A+N +G+
Sbjct: 63  MAPIKSLISIALLALLLLFERLPISAADHGVAPSPLAGVFRLDTLQTAERHQVAENGIGN 122

Query: 61  NLAEKISIKRGVRSAVIAGRASG-SPS----SRAPPFHFGSLLCFCIAF 104
             + +  I+R  R AV   RA G SPS    SR   F  GSLLC C  F
Sbjct: 123 QYSFEQKIRRRARRAV--RRAEGPSPSSANWSRTSSFGVGSLLCLCTVF 169

BLAST of MS002323 vs. ExPASy TrEMBL
Match: A0A6J1GRX2 (uncharacterized protein LOC111456466 OS=Cucurbita moschata OX=3662 GN=LOC111456466 PE=4 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 2.2e-06
Identity = 45/109 (41.28%), Postives = 62/109 (56.88%), Query Frame = 0

Query: 1   MAAVKPFVSIALIALLLFSESLAVSSADYGPDSNSFTGILRRNLLHPSGRRR-ADNSVGS 60
           MA +K  +SIAL+ALLL SE L +S+AD+G   +    + RR+ L  + R + A+N +G 
Sbjct: 90  MAPIKSLISIALLALLLLSEHLPISAADHGVAPSPLARVFRRDTLQTAERHQVAENGIGY 149

Query: 61  NLAEKISIKRGVRSAVIAGRASG-SPS----SRAPPFHFGSLLCFCIAF 104
             + +  I+R  R AV   RA G SPS    S+   F  GSLLC C  F
Sbjct: 150 QYSFEQKIRRRARRAV--RRAEGPSPSSANWSQTSSFGVGSLLCLCTVF 196

BLAST of MS002323 vs. ExPASy TrEMBL
Match: A0A5D3D3Y6 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001920 PE=4 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 4.9e-06
Identity = 45/111 (40.54%), Postives = 57/111 (51.35%), Query Frame = 0

Query: 1   MAAVKPFVSIALIALLLFSESLAVSSADYGPDSNSFTGILRRNLLHPSGRRRADNSVGSN 60
           MAA++P VSIAL+ALL FSESL          S+S TGI  RN+LH    R  + SV S+
Sbjct: 1   MAAIRPLVSIALLALLFFSESLLT--------SHSLTGIPHRNILHTVAHRIVNGSVESS 60

Query: 61  LAEK-----ISIKRGVRSAVIAG-----RASGSPSSRAPPFHFGSLLCFCI 102
              K        +R +R  +I+G       S    SR   F  GS+LC CI
Sbjct: 61  YQRKSNKLHTKDRRRIRPILISGSNGMHHKSAENRSRVASFEVGSMLCICI 103

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6587814.11.0e-2160.58hypothetical protein SDJN03_16379, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG6575859.11.8e-1048.48hypothetical protein SDJN03_26498, partial [Cucurbita argyrosperma subsp. sorori... [more]
KGN65176.17.5e-0949.53hypothetical protein Csa_019975 [Cucumis sativus][more]
KAA0035100.12.4e-0746.73hypothetical protein E6C27_scaffold57G001750 [Cucumis melo var. makuwa] >TYJ9597... [more]
KAG6575858.15.4e-0743.40hypothetical protein SDJN03_26497, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0LYV93.6e-0949.53Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G257330 PE=4 SV=1[more]
A0A5D3BCG41.2e-0746.73Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A6J1JZB11.3e-0641.28uncharacterized protein LOC111488793 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1GRX22.2e-0641.28uncharacterized protein LOC111456466 OS=Cucurbita moschata OX=3662 GN=LOC1114564... [more]
A0A5D3D3Y64.9e-0640.54E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002323.1MS002323.1mRNA