MS002322 (gene) Bitter gourd (TR) v1

BLAST of MS002322 vs. NCBI nr
Match: XP_022135487.1 (eukaryotic translation initiation factor [Momordica charantia])

HSP 1 Score: 1520.8 bits (3936), Expect = 0.0e+00
Identity = 788/797 (98.87%), Postives = 789/797 (99.00%), Query Frame = 0

Query: 1   MQQGDQTVLSLRPGGGRTRLLGPRFDLSSSSSSSSLAFGSFSSDLPALRPHGAPPASFSL 60
           MQQGDQTVLSLRPGGGRTRLLGPRFDLSSSSSSSSLAFGSFSSDLPALRPHGAPPASFSL
Sbjct: 1   MQQGDQTVLSLRPGGGRTRLLGPRFDLSSSSSSSSLAFGSFSSDLPALRPHGAPPASFSL 60

Query: 61  KAGDSRFEGRERVRFTRDQLLQLREIVEVPDDILKLKREIEVEVFGEEQSWSRGESNQPN 120
           KAGDSRFEGRERVRFTRDQLLQLREIVEVPDDILKLKREIEVEVFGEEQSWSRGESNQPN
Sbjct: 61  KAGDSRFEGRERVRFTRDQLLQLREIVEVPDDILKLKREIEVEVFGEEQSWSRGESNQPN 120

Query: 121 QSQNRYSEPDNRDWRGRSAQFSATGEDRSRETIRDRDFGGHFDSRQQEGNQSSRQDSNSS 180
           QSQNRYSEPDNRDWRGRSAQFSATGEDRSRETIRDRDFGGHFDSRQQEGNQSSRQDSNSS
Sbjct: 121 QSQNRYSEPDNRDWRGRSAQFSATGEDRSRETIRDRDFGGHFDSRQQEGNQSSRQDSNSS 180

Query: 181 WTRTTSNQGGAPAALIKAEVPWSTRRGSLSEKDRVLKTVKGILNKLTPEKFDLLKGQLID 240
           WTRTTSNQGGAPAALIKAEVPWS RRGSLSEKDRVLKTVKGILNKLTPEKFDLLKGQLID
Sbjct: 181 WTRTTSNQGGAPAALIKAEVPWSARRGSLSEKDRVLKTVKGILNKLTPEKFDLLKGQLID 240

Query: 241 SGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNLKLPQFPSDEPGGREITFKRVL 300
           SGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNLKLPQFPSDEPGGREITFKRVL
Sbjct: 241 SGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNLKLPQFPSDEPGGREITFKRVL 300

Query: 301 LNICQEAFEGADKLREEVRQMTAPEQEMERRDKERLVKLRTLGNIRLIGELLKQKMVPEK 360
           LNICQEAFEGADKLREEVRQMTAPEQEMERRDKERLVKLRTLGNIRLIGELLKQKMVPEK
Sbjct: 301 LNICQEAFEGADKLREEVRQMTAPEQEMERRDKERLVKLRTLGNIRLIGELLKQKMVPEK 360

Query: 361 IVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENAKSRRINDMYFGRLKELTTH 420
           IVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENAKSRRINDMYFGRLKELTTH
Sbjct: 361 IVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENAKSRRINDMYFGRLKELTTH 420

Query: 421 PQLAPRLKFMVRNVLDLRANNWVPRREEIKAKTITEIHSEAERNLGLRPGATVNIRNSRG 480
           PQLAPRLKFMVRNVLDLRANNWVP      AKTITEIHSEAERNLGLRPGATVNIRNSRG
Sbjct: 421 PQLAPRLKFMVRNVLDLRANNWVPXXXXXXAKTITEIHSEAERNLGLRPGATVNIRNSRG 480

Query: 481 VVSGTAGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGIDNDNWEVPRSRPIPRGGDGS 540
           VVSG+AGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGIDNDNWEVPRSRPIPRGGDGS
Sbjct: 481 VVSGSAGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGIDNDNWEVPRSRPIPRGGDGS 540

Query: 541 GTQPAGRGGQTPLVGKAPMLNARLLPQGTGGSISNKTSALLQGAGASTTRPVNFGPGLEP 600
           GTQPAGRGGQTPLVGKAPMLNARLLPQGTGGSISNKTSALLQGAGASTTRPVNFGPGLEP
Sbjct: 541 GTQPAGRGGQTPLVGKAPMLNARLLPQGTGGSISNKTSALLQGAGASTTRPVNFGPGLEP 600

Query: 601 SSQVQKPVTAAAVLPLAEKPQVPAASSKQDDLEKKTLSLLEEYFSVRLLDEALQCVEELV 660
           SSQVQKPVTAAAVLPLAEKPQVPAASSKQDDLEKKTLSLLEEYFSVRLLDEALQCVEELV
Sbjct: 601 SSQVQKPVTAAAVLPLAEKPQVPAASSKQDDLEKKTLSLLEEYFSVRLLDEALQCVEELV 660

Query: 661 SPAYHPEIVKQAISLALEKIPPCVEAVVKLLDYLLSKKVFTAGDIGTGCLQYASEIDDAG 720
           SPAYHPEIVKQAISLALEKIPPCVEAVVKLLDYLLSKKVFTAGDIGTGCLQYASEIDD G
Sbjct: 661 SPAYHPEIVKQAISLALEKIPPCVEAVVKLLDYLLSKKVFTAGDIGTGCLQYASEIDDVG 720

Query: 721 IDLPKAPMNFGEIIGKLVISGGLDFQVVKEALKKVEDDRFRKDIFDAVLKAVSSNASGQG 780
           IDLPKAPMNFGEIIGKLVISGGLDFQVVKEALKKVEDDRFRKDIFDAVLKAVSSNASGQG
Sbjct: 721 IDLPKAPMNFGEIIGKLVISGGLDFQVVKEALKKVEDDRFRKDIFDAVLKAVSSNASGQG 780

Query: 781 LSDSLESDIEACRSLAY 798
           LSDSLESDIEACRSLAY
Sbjct: 781 LSDSLESDIEACRSLAY 797

BLAST of MS002322 vs. NCBI nr
Match: XP_023515804.1 (eukaryotic translation initiation factor [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1354.3 bits (3504), Expect = 0.0e+00
Identity = 696/800 (87.00%), Postives = 739/800 (92.38%), Query Frame = 0

Query: 1   MQQGDQTVLSLRPGGGRTRLLGPRFDL---SSSSSSSSLAFGSFSSDLPALRPHGAPPAS 60
           MQQGDQTVLSLRPGGGR RLLGPRF+L   SSSSSSSS AFGSFSSDLP LRPHGA  +S
Sbjct: 1   MQQGDQTVLSLRPGGGRPRLLGPRFELSSSSSSSSSSSFAFGSFSSDLPTLRPHGASSSS 60

Query: 61  FSLKAGDSRFEGRERVRFTRDQLLQLREIVEVPDDILKLKREIEVEVFGEEQSWSRGESN 120
           FS+KAGDSRFEGRERVR+TRDQLLQL+E+VEVPDDILK+KR+IEVE+FGEEQSWSRGESN
Sbjct: 61  FSVKAGDSRFEGRERVRYTRDQLLQLQEVVEVPDDILKIKRDIEVEIFGEEQSWSRGESN 120

Query: 121 QPNQSQNRYSEPDNRDWRGRSAQFSATGEDRSRETIRDRDFGGHFDSRQQEGNQSSRQDS 180
           Q N SQNRYSEPDNRDWRGRSAQFSA+GEDRSRETIRDRDFGGHFDSRQQ+ NQ +RQD 
Sbjct: 121 QLNPSQNRYSEPDNRDWRGRSAQFSASGEDRSRETIRDRDFGGHFDSRQQDANQLNRQDP 180

Query: 181 NSSWTRTTSNQGGAPAALIKAEVPWSTRRGSLSEKDRVLKTVKGILNKLTPEKFDLLKGQ 240
           +SSWTRTT NQGG P ALIKAEVPWS RRG+LS+KDRVLKTVKGILNKLTPEKFDLLKGQ
Sbjct: 181 HSSWTRTTPNQGGGPPALIKAEVPWSARRGTLSDKDRVLKTVKGILNKLTPEKFDLLKGQ 240

Query: 241 LIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNLKLPQFPSDEPGGREITFK 300
           LIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLN KLPQFPSDEPGGRE+TFK
Sbjct: 241 LIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNQKLPQFPSDEPGGREVTFK 300

Query: 301 RVLLNICQEAFEGADKLREEVRQMTAPEQEMERRDKERLVKLRTLGNIRLIGELLKQKMV 360
           RVLLNICQEAFEGADKLREE+RQMTAPEQE ERRDK+RL+KLRTLGNIRLIGELLKQKMV
Sbjct: 301 RVLLNICQEAFEGADKLREELRQMTAPEQESERRDKDRLLKLRTLGNIRLIGELLKQKMV 360

Query: 361 PEKIVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENAKSRRINDMYFGRLKEL 420
           PEKIVHHIVQELLGPDNK+CPAEENVEAICQFFNTIGKQLDEN KSRRINDMYFGRLKEL
Sbjct: 361 PEKIVHHIVQELLGPDNKSCPAEENVEAICQFFNTIGKQLDENTKSRRINDMYFGRLKEL 420

Query: 421 TTHPQLAPRLKFMVRNVLDLRANNWVPRREEIKAKTITEIHSEAERNLGLRPGATVNIRN 480
           TTH QLAPRLKFMVR+VLDLRANNWVPRREE+KAKTITEIH+EAE+NLGLRPGATVN+RN
Sbjct: 421 TTHTQLAPRLKFMVRDVLDLRANNWVPRREEVKAKTITEIHTEAEKNLGLRPGATVNMRN 480

Query: 481 SRGVVSGTAGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGIDNDNWEVPRSRPIPRGG 540
           +RGVVSG+ GNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPG+DNDNWEV R+RPIPRG 
Sbjct: 481 NRGVVSGSPGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGMDNDNWEVHRTRPIPRGA 540

Query: 541 DGSGTQPAGRGGQTPLVGKAPMLNARLLPQGTGGSISNKTSALLQGAGASTTRPVNFGPG 600
           DGSG       G    VGK   LNARLLPQGTGG ISNK SALLQGAGASTTRPVNFG G
Sbjct: 541 DGSGR------GSASFVGKGSALNARLLPQGTGGLISNKNSALLQGAGASTTRPVNFGHG 600

Query: 601 LEPSSQVQKPVTAAAVLPLAEKPQVPAASSKQDDLEKKTLSLLEEYFSVRLLDEALQCVE 660
           LEPS QV KP+ A  V P AE+PQVPAAS KQDDL KKT SLLEEYFS+R+LDEALQCVE
Sbjct: 601 LEPSGQVHKPIAAVTVPPPAERPQVPAASLKQDDLRKKTHSLLEEYFSIRILDEALQCVE 660

Query: 661 ELVSPAYHPEIVKQAISLALEKIPPCVEAVVKLLDYLLSKKVFTAGDIGTGCLQYASEID 720
           EL+SPAYHPE+VK+AISLALEK PPCVEAVVK LDYLLS+KVFTA DIGTGCL YAS +D
Sbjct: 661 ELMSPAYHPEVVKEAISLALEKSPPCVEAVVKFLDYLLSRKVFTASDIGTGCLLYASMLD 720

Query: 721 DAGIDLPKAPMNFGEIIGKLVISGGLDFQVVKEALKKVEDDRFRKDIFDAVLKAVSSNAS 780
           DAGIDLPKAP+NFGEIIGKLVISGGLDFQVVKEA+KKVEDDRFRKDIFDAVLK +SSN S
Sbjct: 721 DAGIDLPKAPINFGEIIGKLVISGGLDFQVVKEAIKKVEDDRFRKDIFDAVLKTISSNVS 780

Query: 781 GQGLSDSLESDIEACRSLAY 798
           GQGL DS+ SDIE CR+LAY
Sbjct: 781 GQGLLDSMASDIEGCRNLAY 794

BLAST of MS002322 vs. NCBI nr
Match: KAG6589764.1 (Eukaryotic translation initiation factor isoform 4G-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1354.3 bits (3504), Expect = 0.0e+00
Identity = 697/800 (87.12%), Postives = 739/800 (92.38%), Query Frame = 0

Query: 1   MQQGDQTVLSLRPGGGRTRLLGPRFDL---SSSSSSSSLAFGSFSSDLPALRPHGAPPAS 60
           MQQGDQTVLSLRPGGGR RLLGPRF+L   SSSSSSSS AFGSFSSDLP LRPHGA  +S
Sbjct: 1   MQQGDQTVLSLRPGGGRPRLLGPRFELSSSSSSSSSSSFAFGSFSSDLPTLRPHGASSSS 60

Query: 61  FSLKAGDSRFEGRERVRFTRDQLLQLREIVEVPDDILKLKREIEVEVFGEEQSWSRGESN 120
           FS+KAGDSRFEGRERVR+TRDQLLQL+E+VEVPDDILK+KR+IEVE+FGEEQSWSRGESN
Sbjct: 61  FSVKAGDSRFEGRERVRYTRDQLLQLQEVVEVPDDILKIKRDIEVEIFGEEQSWSRGESN 120

Query: 121 QPNQSQNRYSEPDNRDWRGRSAQFSATGEDRSRETIRDRDFGGHFDSRQQEGNQSSRQDS 180
           Q N SQNRYSEPDNRDWRGRSAQFSA+GEDRSRETIRDRDFGGHFDSRQQ+ NQ +RQD 
Sbjct: 121 QLNPSQNRYSEPDNRDWRGRSAQFSASGEDRSRETIRDRDFGGHFDSRQQDANQLNRQDP 180

Query: 181 NSSWTRTTSNQGGAPAALIKAEVPWSTRRGSLSEKDRVLKTVKGILNKLTPEKFDLLKGQ 240
           +SSWTRTT NQGG P ALIKAEVPWS RRG+LS+KDRVLKTVKGILNKLTPEKFDLLKGQ
Sbjct: 181 HSSWTRTTPNQGGGPPALIKAEVPWSARRGTLSDKDRVLKTVKGILNKLTPEKFDLLKGQ 240

Query: 241 LIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNLKLPQFPSDEPGGREITFK 300
           LIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLN KLPQFPSDEPGGRE+TFK
Sbjct: 241 LIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNQKLPQFPSDEPGGREVTFK 300

Query: 301 RVLLNICQEAFEGADKLREEVRQMTAPEQEMERRDKERLVKLRTLGNIRLIGELLKQKMV 360
           RVLLNICQEAFEGADKLREE+RQMTAPEQE ERRDK+RL+KLRTLGNIRLIGELLKQKMV
Sbjct: 301 RVLLNICQEAFEGADKLREELRQMTAPEQESERRDKDRLLKLRTLGNIRLIGELLKQKMV 360

Query: 361 PEKIVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENAKSRRINDMYFGRLKEL 420
           PEKIVHHIVQELLGPDNK+CPAEENVEAICQFFNTIGKQLDEN KSRRINDMYFGRLKEL
Sbjct: 361 PEKIVHHIVQELLGPDNKSCPAEENVEAICQFFNTIGKQLDENTKSRRINDMYFGRLKEL 420

Query: 421 TTHPQLAPRLKFMVRNVLDLRANNWVPRREEIKAKTITEIHSEAERNLGLRPGATVNIRN 480
           TTH QLAPRLKFMVR+VLDLRANNWVPRREE+KAKTITEIH+EAE+NLGLRPGATVN+RN
Sbjct: 421 TTHTQLAPRLKFMVRDVLDLRANNWVPRREEVKAKTITEIHTEAEKNLGLRPGATVNMRN 480

Query: 481 SRGVVSGTAGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGIDNDNWEVPRSRPIPRGG 540
           +RGVVSG+ GNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPG+DNDNWEV R+RPIPRG 
Sbjct: 481 NRGVVSGSPGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGMDNDNWEVHRTRPIPRGA 540

Query: 541 DGSGTQPAGRGGQTPLVGKAPMLNARLLPQGTGGSISNKTSALLQGAGASTTRPVNFGPG 600
           DGSG       G    VGK   LNARLLPQGTGG ISNK SALLQGAGASTTRPVNFG G
Sbjct: 541 DGSGR------GSASFVGKGSALNARLLPQGTGGLISNKNSALLQGAGASTTRPVNFGHG 600

Query: 601 LEPSSQVQKPVTAAAVLPLAEKPQVPAASSKQDDLEKKTLSLLEEYFSVRLLDEALQCVE 660
           LEPS QV KP+ AA V P AE+PQVPAAS KQDDL KKT SLLEEYFS+R+LDEALQCVE
Sbjct: 601 LEPSGQVHKPIAAATVPPPAERPQVPAASLKQDDLRKKTHSLLEEYFSIRILDEALQCVE 660

Query: 661 ELVSPAYHPEIVKQAISLALEKIPPCVEAVVKLLDYLLSKKVFTAGDIGTGCLQYASEID 720
           EL+SPAYHPE+VK+AISLALEK PPCVEAVVK LDYLLS+KVFTA DIGTGCL YAS +D
Sbjct: 661 ELMSPAYHPEVVKEAISLALEKSPPCVEAVVKFLDYLLSRKVFTASDIGTGCLLYASMLD 720

Query: 721 DAGIDLPKAPMNFGEIIGKLVISGGLDFQVVKEALKKVEDDRFRKDIFDAVLKAVSSNAS 780
           DA IDLPKAP+NFGEIIGKLVISGGLDFQVVKEA+KKVEDDRFRKDIFDAVLK +SSN S
Sbjct: 721 DAAIDLPKAPINFGEIIGKLVISGGLDFQVVKEAIKKVEDDRFRKDIFDAVLKTISSNVS 780

Query: 781 GQGLSDSLESDIEACRSLAY 798
           GQGL DSL SDIE CR+LAY
Sbjct: 781 GQGLLDSLASDIEGCRNLAY 794

BLAST of MS002322 vs. NCBI nr
Match: XP_022921731.1 (eukaryotic translation initiation factor [Cucurbita moschata])

HSP 1 Score: 1352.4 bits (3499), Expect = 0.0e+00
Identity = 697/797 (87.45%), Postives = 739/797 (92.72%), Query Frame = 0

Query: 1   MQQGDQTVLSLRPGGGRTRLLGPRFDLSSSSSSSSLAFGSFSSDLPALRPHGAPPASFSL 60
           MQQGDQTVLSLRPGGGR RLLGPRF+L SSSSSSS AFGSFSSDLP LRPHGA  +SFS+
Sbjct: 1   MQQGDQTVLSLRPGGGRPRLLGPRFEL-SSSSSSSFAFGSFSSDLPTLRPHGASSSSFSV 60

Query: 61  KAGDSRFEGRERVRFTRDQLLQLREIVEVPDDILKLKREIEVEVFGEEQSWSRGESNQPN 120
           KAGDSRFEGRERVR+TRDQLLQL+E+VEVPDDILK+KR+IEVE+FGEEQSWSRGESNQ N
Sbjct: 61  KAGDSRFEGRERVRYTRDQLLQLQEVVEVPDDILKIKRDIEVEIFGEEQSWSRGESNQLN 120

Query: 121 QSQNRYSEPDNRDWRGRSAQFSATGEDRSRETIRDRDFGGHFDSRQQEGNQSSRQDSNSS 180
            SQNRYSEPDNRDWRGRSAQFSA+GEDRSRETIRDRDFGGHFDSRQQ+ NQ +RQD +SS
Sbjct: 121 PSQNRYSEPDNRDWRGRSAQFSASGEDRSRETIRDRDFGGHFDSRQQDANQLNRQD-HSS 180

Query: 181 WTRTTSNQGGAPAALIKAEVPWSTRRGSLSEKDRVLKTVKGILNKLTPEKFDLLKGQLID 240
           WTRTT NQGG P ALIKAEVPWS RRG+LS+KDRVLKTVKGILNKLTPEKFDLLKGQLID
Sbjct: 181 WTRTTPNQGGGPPALIKAEVPWSARRGTLSDKDRVLKTVKGILNKLTPEKFDLLKGQLID 240

Query: 241 SGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNLKLPQFPSDEPGGREITFKRVL 300
           SGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLN KLPQFPSDEPGGRE+TFKRVL
Sbjct: 241 SGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNQKLPQFPSDEPGGREVTFKRVL 300

Query: 301 LNICQEAFEGADKLREEVRQMTAPEQEMERRDKERLVKLRTLGNIRLIGELLKQKMVPEK 360
           LNICQEAFEGADKLREE+RQMTAPEQE ERRDK+RL+KLRTLGNIRLIGELLKQKMVPEK
Sbjct: 301 LNICQEAFEGADKLREELRQMTAPEQESERRDKDRLLKLRTLGNIRLIGELLKQKMVPEK 360

Query: 361 IVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENAKSRRINDMYFGRLKELTTH 420
           IVHHIVQELLGPDNK+CPAEENVEAICQFFNTIGKQLDEN KSRRINDMYFGRLKELTTH
Sbjct: 361 IVHHIVQELLGPDNKSCPAEENVEAICQFFNTIGKQLDENTKSRRINDMYFGRLKELTTH 420

Query: 421 PQLAPRLKFMVRNVLDLRANNWVPRREEIKAKTITEIHSEAERNLGLRPGATVNIRNSRG 480
            QLAPRLKFMVR+VLDLRANNWVPRREE+KAKTITEIH+EAE+NLGLRPGATVN+RN+RG
Sbjct: 421 TQLAPRLKFMVRDVLDLRANNWVPRREEVKAKTITEIHTEAEKNLGLRPGATVNMRNNRG 480

Query: 481 VVSGTAGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGIDNDNWEVPRSRPIPRGGDGS 540
           VVSG+ GNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPG+DNDNWEV R+RPIPRG DGS
Sbjct: 481 VVSGSPGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGMDNDNWEVHRTRPIPRGADGS 540

Query: 541 GTQPAGRGGQTPLVGKAPMLNARLLPQGTGGSISNKTSALLQGAGASTTRPVNFGPGLEP 600
           G       G    VGK   LNARLLPQGTGG ISNK SALLQGAGASTTRPVNFG GLEP
Sbjct: 541 GR------GSASFVGKGSALNARLLPQGTGGLISNKNSALLQGAGASTTRPVNFGHGLEP 600

Query: 601 SSQVQKPVTAAAVLPLAEKPQVPAASSKQDDLEKKTLSLLEEYFSVRLLDEALQCVEELV 660
           S QV KP+ AA V P AE+PQVPAAS KQDDL KKT SLLEEYFS+R+LDEALQCVEEL+
Sbjct: 601 SGQVHKPIAAATVPPPAERPQVPAASLKQDDLRKKTHSLLEEYFSIRILDEALQCVEELM 660

Query: 661 SPAYHPEIVKQAISLALEKIPPCVEAVVKLLDYLLSKKVFTAGDIGTGCLQYASEIDDAG 720
           SPAYHPE+VK+AISLALEK PPCVEAVVK LDYLLS+KVFTA DIGTGCL YAS +DDAG
Sbjct: 661 SPAYHPEVVKEAISLALEKSPPCVEAVVKFLDYLLSRKVFTASDIGTGCLLYASMLDDAG 720

Query: 721 IDLPKAPMNFGEIIGKLVISGGLDFQVVKEALKKVEDDRFRKDIFDAVLKAVSSNASGQG 780
           IDLPKAP+NFGEIIGKLVISGGLDFQVVKEA+KKVEDDRFRKDIFDAVLK +SSN SGQG
Sbjct: 721 IDLPKAPINFGEIIGKLVISGGLDFQVVKEAIKKVEDDRFRKDIFDAVLKTISSNVSGQG 780

Query: 781 LSDSLESDIEACRSLAY 798
           L DSL SDIE CR+LAY
Sbjct: 781 LLDSLASDIEGCRNLAY 789

BLAST of MS002322 vs. NCBI nr
Match: XP_022988549.1 (eukaryotic translation initiation factor [Cucurbita maxima])

HSP 1 Score: 1347.4 bits (3486), Expect = 0.0e+00
Identity = 691/800 (86.38%), Postives = 738/800 (92.25%), Query Frame = 0

Query: 1   MQQGDQTVLSLRPGGGRTRLLGPRFDL---SSSSSSSSLAFGSFSSDLPALRPHGAPPAS 60
           MQQGDQTVLSLRPGGGR RLLGPRF+L   SSSSSSSS AFGSFSSDLP+LRPHGA  +S
Sbjct: 1   MQQGDQTVLSLRPGGGRPRLLGPRFELSSSSSSSSSSSFAFGSFSSDLPSLRPHGASSSS 60

Query: 61  FSLKAGDSRFEGRERVRFTRDQLLQLREIVEVPDDILKLKREIEVEVFGEEQSWSRGESN 120
           FS+KAGDSRFEGRERVR+TRDQLLQL+E VEVPDDILK+KR+IEVE+FGEEQSWSRGESN
Sbjct: 61  FSVKAGDSRFEGRERVRYTRDQLLQLQEAVEVPDDILKIKRDIEVEIFGEEQSWSRGESN 120

Query: 121 QPNQSQNRYSEPDNRDWRGRSAQFSATGEDRSRETIRDRDFGGHFDSRQQEGNQSSRQDS 180
           Q N SQNRYSEPDNRDWRGRSAQFS++GEDRSRETIRDRDFGGHFDSRQQ+ NQ +RQD 
Sbjct: 121 QLNLSQNRYSEPDNRDWRGRSAQFSSSGEDRSRETIRDRDFGGHFDSRQQDANQLNRQDP 180

Query: 181 NSSWTRTTSNQGGAPAALIKAEVPWSTRRGSLSEKDRVLKTVKGILNKLTPEKFDLLKGQ 240
           +SSWTRTT NQGG P ALIKAEVPWS RRG+LS+KDRVLKTVKGILNKLTPEKFDLLKGQ
Sbjct: 181 HSSWTRTTPNQGGGPPALIKAEVPWSARRGTLSDKDRVLKTVKGILNKLTPEKFDLLKGQ 240

Query: 241 LIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNLKLPQFPSDEPGGREITFK 300
           LIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLN KLPQFPSDEPGGRE+TFK
Sbjct: 241 LIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNQKLPQFPSDEPGGREVTFK 300

Query: 301 RVLLNICQEAFEGADKLREEVRQMTAPEQEMERRDKERLVKLRTLGNIRLIGELLKQKMV 360
           RVLLNICQEAFEGADKLREE+RQMTAPEQE ERRDK+RL+KLRTLGNIRLIGELLKQKMV
Sbjct: 301 RVLLNICQEAFEGADKLREELRQMTAPEQESERRDKDRLLKLRTLGNIRLIGELLKQKMV 360

Query: 361 PEKIVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENAKSRRINDMYFGRLKEL 420
           PEKIVHHIVQELLGPDNK+CPAEENVEAICQFFNTIGKQLDEN KSRRINDMYFGRLKEL
Sbjct: 361 PEKIVHHIVQELLGPDNKSCPAEENVEAICQFFNTIGKQLDENTKSRRINDMYFGRLKEL 420

Query: 421 TTHPQLAPRLKFMVRNVLDLRANNWVPRREEIKAKTITEIHSEAERNLGLRPGATVNIRN 480
           TTH QLAPRLKFMVR+VL+LRANNWVPRREE+KAKTITEIH+EAE+NLGLRPGATVN+RN
Sbjct: 421 TTHTQLAPRLKFMVRDVLELRANNWVPRREEVKAKTITEIHTEAEKNLGLRPGATVNMRN 480

Query: 481 SRGVVSGTAGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGIDNDNWEVPRSRPIPRGG 540
           +RGVVSG+ GNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPG+DNDNWEV R+RPIPRG 
Sbjct: 481 NRGVVSGSPGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGMDNDNWEVHRTRPIPRGA 540

Query: 541 DGSGTQPAGRGGQTPLVGKAPMLNARLLPQGTGGSISNKTSALLQGAGASTTRPVNFGPG 600
           DGSG       G     GK   LNARLLPQGTGG ISNK SALLQGAGA+TTRPVNFG G
Sbjct: 541 DGSGR------GSASFAGKGSALNARLLPQGTGGLISNKNSALLQGAGATTTRPVNFGHG 600

Query: 601 LEPSSQVQKPVTAAAVLPLAEKPQVPAASSKQDDLEKKTLSLLEEYFSVRLLDEALQCVE 660
           LEPS QV KP+ AA V P AE+PQ+PAAS KQDDL KKT SLLEEYFS+R+LDEALQCVE
Sbjct: 601 LEPSGQVHKPIAAATVPPPAERPQIPAASLKQDDLRKKTHSLLEEYFSIRILDEALQCVE 660

Query: 661 ELVSPAYHPEIVKQAISLALEKIPPCVEAVVKLLDYLLSKKVFTAGDIGTGCLQYASEID 720
           EL++P YHPE+VK+AISLALEK PPCVEAVVK LDYLLS+KVFTA D+GTGCL YAS +D
Sbjct: 661 ELMTPTYHPEVVKEAISLALEKSPPCVEAVVKFLDYLLSRKVFTASDVGTGCLLYASMLD 720

Query: 721 DAGIDLPKAPMNFGEIIGKLVISGGLDFQVVKEALKKVEDDRFRKDIFDAVLKAVSSNAS 780
           DAGIDLPKAP+NFGEIIGKLVISGGLDFQVVKEA+KKVEDDRFRKDIFDAVLK +SSN S
Sbjct: 721 DAGIDLPKAPINFGEIIGKLVISGGLDFQVVKEAIKKVEDDRFRKDIFDAVLKTISSNVS 780

Query: 781 GQGLSDSLESDIEACRSLAY 798
           GQGL DSL SDIE CRSLAY
Sbjct: 781 GQGLLDSLASDIEGCRSLAY 794

BLAST of MS002322 vs. ExPASy Swiss-Prot
Match: Q93ZT6 (Eukaryotic translation initiation factor isoform 4G-1 OS=Arabidopsis thaliana OX=3702 GN=EIF(ISO)4G1 PE=1 SV=1)

HSP 1 Score: 927.5 bits (2396), Expect = 9.8e-269
Identity = 516/807 (63.94%), Postives = 627/807 (77.70%), Query Frame = 0

Query: 1   MQQGDQTVLSLRPGGGR-TRLLGPRFDLSSSSSSSSLAFGSF-SSDLPALRPH-GAPPAS 60
           MQQGDQTVLSLRPGGGR  RL G      SSSSSSSL+FGS  SSDLP LRPH GAP +S
Sbjct: 1   MQQGDQTVLSLRPGGGRGNRLFG------SSSSSSSLSFGSLSSSDLPLLRPHGGAPASS 60

Query: 61  FSLKAGDSRFEGRERVRFTRDQLLQLREIVEVPDDILKLKREIEVEVFGEEQSWSRGESN 120
           F  K GDSRF+GRERV++TR+QLL+L+E  ++ D+IL+++RE   E+FGEE +W+RGES 
Sbjct: 61  FPFKGGDSRFDGRERVKYTREQLLELKETTQLSDEILRVQRETAAELFGEEGTWARGESV 120

Query: 121 QPN----QSQNRYSEPDNRDWRGRSAQFSATGEDRSRETIRDRDFGGHFDSRQQEGNQSS 180
             N    QS +R+SEPD+RDWR RS Q   +GE+RS + +R+       DSR  E +Q +
Sbjct: 121 VSNLVPVQSASRFSEPDSRDWRSRSTQPPPSGEERSWDNLREAK-----DSRYVEASQYN 180

Query: 181 RQDS-NSSWTRT--TSNQGGAPA-ALIKAEVPWSTRRGSLSEKDRVLKTVKGILNKLTPE 240
           RQD  NS ++R   +SNQGG PA  L+KAEVPWS RRG+LSE DRVLKTVKGILNKLTPE
Sbjct: 181 RQDQPNSQFSRANISSNQGGGPAPVLVKAEVPWSARRGNLSENDRVLKTVKGILNKLTPE 240

Query: 241 KFDLLKGQLIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNLKLPQFPSDEP 300
           K+DLLKGQLI+SGITSADILK VI+LIFDKAVLEPTFCPMYA+LCSD+N +LP FP  EP
Sbjct: 241 KYDLLKGQLIESGITSADILKGVITLIFDKAVLEPTFCPMYAKLCSDINDQLPTFPPAEP 300

Query: 301 GGREITFKRVLLNICQEAFEGADKLREEVRQMTAPEQEMERRDKERLVKLRTLGNIRLIG 360
           G +EITFKRVLLNICQEAFEGA +LREE+RQM+AP+QE ER DKE+L+KL+TLGNIRLIG
Sbjct: 301 GDKEITFKRVLLNICQEAFEGASQLREELRQMSAPDQEAERNDKEKLLKLKTLGNIRLIG 360

Query: 361 ELLKQKMVPEKIVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENAKSRRINDM 420
           ELLKQKMVPEKIVHHIVQELLG D K CPAEENVEAIC FF TIGKQLD N KS+RIND+
Sbjct: 361 ELLKQKMVPEKIVHHIVQELLGADEKVCPAEENVEAICHFFKTIGKQLDGNVKSKRINDV 420

Query: 421 YFGRLKELTTHPQLAPRLKFMVRNVLDLRANNWVPRREEIKAKTITEIHSEAERNLGLRP 480
           YF RL+ L+ +PQL  RL+FMV+N++D+R+N WVPRREE+KA+TITEIH+EAE+NLGLRP
Sbjct: 421 YFKRLQALSKNPQLELRLRFMVQNIIDMRSNGWVPRREEMKARTITEIHTEAEKNLGLRP 480

Query: 481 GATVNIRNSRGVVSGTAGNTSPGG-FPINRPGFGGMMPGMPGTRKMPGMPGIDNDNWEVP 540
           GAT N+R  RG+VS + G  SPG  +P  RPG GG+MPGMPGTR+MPGMPG+DNDNWEVP
Sbjct: 481 GATANMR--RGMVS-SGGPVSPGPVYPGGRPGAGGLMPGMPGTRRMPGMPGVDNDNWEVP 540

Query: 541 RSRPIPRGGDGSGTQPAGRGGQTPLVGKAPMLNARLLPQGTGGSISNKTSALLQGAGAST 600
           R+R + R  DG G         +P V K+  +N RLLPQG+ G +S KTSALLQG+G S 
Sbjct: 541 RTRSMSR-RDGPGPL------HSPAVSKSASMNTRLLPQGSSGIMSGKTSALLQGSG-SV 600

Query: 601 TRPVNFGPGLEPSSQVQKPVTAAAVLPLAEKPQVPAASSKQDDLEKKTLSLLEEYFSVRL 660
           +RPV      E  +Q   P+T    +   EKPQ       ++ L++KT SLLEEYF+VRL
Sbjct: 601 SRPVTV--SAERPAQSVAPLTVPVPV---EKPQPSGPKLSEEVLQRKTKSLLEEYFNVRL 660

Query: 661 LDEALQCVEELVSPAYHPEIVKQAISLALEKIPPCVEAVVKLLDYLLSKKVFTAGDIGTG 720
           L EALQCVEEL  P+YHPE VK+AISL+LEK PP VE +  LL+YLLSKKV    D+ TG
Sbjct: 661 LGEALQCVEELGLPSYHPEFVKEAISLSLEKSPPVVEPIATLLEYLLSKKVVAPKDLETG 720

Query: 721 CLQYASEIDDAGIDLPKAPMNFGEIIGKLVISGGLDFQVVKEALKKVEDDRFRKDIFDAV 780
            L Y + +DD GIDLPKAP NFGEI+GKL+++GG+DF++V+E + K+EDDRF+K + DA 
Sbjct: 721 FLLYGAMLDDIGIDLPKAPNNFGEIVGKLILAGGVDFKLVREIIGKMEDDRFQKMVVDAA 780

Query: 781 LKAVSSNASGQGLSDSLESDIEACRSL 796
           ++ V S+  G+ L  S  +DIEACR+L
Sbjct: 781 VRIVESSEQGKSLLASQAADIEACRNL 780

BLAST of MS002322 vs. ExPASy Swiss-Prot
Match: Q84PB3 (Eukaryotic translation initiation factor isoform 4G-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0499300 PE=2 SV=2)

HSP 1 Score: 840.1 bits (2169), Expect = 2.1e-242
Identity = 488/819 (59.58%), Postives = 594/819 (72.53%), Query Frame = 0

Query: 1   MQQGDQTVLSLRPGGGRTRLLGPRFDLSSSSSSSSLAFGSFSSDLPALRPHGAPPASFSL 60
           M++  Q V+SLRPGGG     GPR     S + ++ A G  S DL  LR H       + 
Sbjct: 1   MEKDHQPVISLRPGGGGG---GPRPGRLFSPAFAAAASG--SGDL--LRSH----VGGAS 60

Query: 61  KAGDSRFEGRERVRFTRDQLLQLREIVEVPDDILKLKREIEVEVFGEEQSWSRGESNQPN 120
           K GD  FE RERVR+TRDQLL+LREIV++P+ IL++ +EI++E+ GE+Q W R ES+   
Sbjct: 61  KIGDPNFEVRERVRYTRDQLLELREIVDIPEAILRINQEIDIELHGEDQIWGRPESDVQV 120

Query: 121 QSQ------NRYSEPDNRDWRGRSAQFSATGEDRSRETIRDRDFGGHFDSRQQEGNQSSR 180
           Q+Q      NRY E DNRDWR R+ Q  A  E++S + IR+         RQQE  Q +R
Sbjct: 121 QTQTQAQPHNRYGETDNRDWRARTVQPPAANEEKSWDNIREAKAAHASSGRQQE--QVNR 180

Query: 181 QDSNSSWTRTTSNQGGAPAALIKAEVPWSTRRGSLSEKDRVLKTVKGILNKLTPEKFDLL 240
           QD  +    + +  G  P ALIKAEVPWS RRG+LSEKDRVLKTVKGILNKLTPEKFDLL
Sbjct: 181 QDQLNHQFASKAQVGPTP-ALIKAEVPWSARRGNLSEKDRVLKTVKGILNKLTPEKFDLL 240

Query: 241 KGQLIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNLKLPQFPSDEPGGREI 300
           KGQL++SGIT+ADILKDVISLIF+KAV EPTFCPMYAQLCSDLN KLP FPS+EPGG+EI
Sbjct: 241 KGQLMESGITTADILKDVISLIFEKAVFEPTFCPMYAQLCSDLNEKLPSFPSEEPGGKEI 300

Query: 301 TFKRVLLNICQEAFEGADKLREEVRQMTAPEQEMERRDKERLVKLRTLGNIRLIGELLKQ 360
           TFKRVLLN CQEAFEGA+ LR E+ ++T P+QEMERRDKER+VKLRTLGNIRLIGELLKQ
Sbjct: 301 TFKRVLLNNCQEAFEGAESLRAEIAKLTGPDQEMERRDKERIVKLRTLGNIRLIGELLKQ 360

Query: 361 KMVPEKIVHHIVQELL--GPDNKTCPAEENVEAICQFFNTIGKQLDENAKSRRINDMYFG 420
           KMVPEKIVHHIVQELL  GPD K CP EENVEAICQFFNTIGKQLDEN KSRRIND YF 
Sbjct: 361 KMVPEKIVHHIVQELLGSGPDKKACPEEENVEAICQFFNTIGKQLDENPKSRRINDTYFI 420

Query: 421 RLKELTTHPQLAPRLKFMVRNVLDLRANNWVPRREEIKAKTITEIHSEAERNLGLRPGAT 480
           ++KELTT+ QLAPRL+FMVR+V+DLR+NNWVPRREEIKAKTI+EIH EA + LGLRPGAT
Sbjct: 421 QMKELTTNLQLAPRLRFMVRDVVDLRSNNWVPRREEIKAKTISEIHDEAMKTLGLRPGAT 480

Query: 481 VNIRNSRGVVSGTAGNTSPGGFPINRPGFGGMMPGMPGT------RKMPGMPGIDNDNWE 540
              RN R   +   G  SPGGFP+NRPG GGMMPGMPGT      RKMPGMPG+DNDNWE
Sbjct: 481 GLTRNGR---NAPGGPLSPGGFPMNRPGTGGMMPGMPGTPGMPGSRKMPGMPGLDNDNWE 540

Query: 541 VPRSRPIPRGGDGSGTQPAGRGGQTPLVGKAPMLNARLLPQGTGGSISNKTSALLQGAGA 600
           VPRS+ +PRG       P     +   + K   +N+RLLP G+G  I    SALL G+G 
Sbjct: 541 VPRSKSMPRGDSLRNQGPL--LNKPSSINKPSSINSRLLPHGSGALIGK--SALL-GSGG 600

Query: 601 STTRPVNFGPGL----EPSSQVQKPVTAA-AVLPLAEKP-----QVPAASSKQDDLEKKT 660
             +RP +    L      ++   KPV+AA AV+P+ +K      ++PAA      ++KKT
Sbjct: 601 PPSRPSSLMASLTHTPAQTAPSPKPVSAAPAVVPVTDKAAGSSHEMPAA------VQKKT 660

Query: 661 LSLLEEYFSVRLLDEALQCVEELVSPAYHPEIVKQAISLALEKIPPCVEAVVKLLDYLLS 720
           +SLLEEYF +R+LDEA QC+EEL  P Y+ EIVK+AI+LAL+K P  ++ +V+LL++L +
Sbjct: 661 VSLLEEYFGIRILDEAQQCIEELQCPEYYSEIVKEAINLALDKGPNFIDPLVRLLEHLHA 720

Query: 721 KKVFTAGDIGTGCLQYASEIDDAGIDLPKAPMNFGEIIGKLVISGGLDFQVVKEALKKVE 780
           KK+F   D+ TGCL YA+ ++D GIDLP AP  FGE++ +L +S GL F+VV+E LK VE
Sbjct: 721 KKIFKTEDLKTGCLLYAALLEDIGIDLPLAPALFGEVVARLSLSCGLSFEVVEEILKAVE 780

Query: 781 DDRFRKDIFDAVLKAVSSNASGQGLSDSLESDIEACRSL 796
           D  FRK IFDAV+K +  N+SGQ +  S    I+AC  L
Sbjct: 781 DTYFRKGIFDAVMKTMGGNSSGQAILSSHAVVIDACNKL 791

BLAST of MS002322 vs. ExPASy Swiss-Prot
Match: Q03387 (Eukaryotic translation initiation factor isoform 4G-1 OS=Triticum aestivum OX=4565 PE=1 SV=2)

HSP 1 Score: 833.2 bits (2151), Expect = 2.5e-240
Identity = 475/811 (58.57%), Postives = 580/811 (71.52%), Query Frame = 0

Query: 5   DQTVLSLRPGGG-----RTRLLGPRFDLSSSSSSSSLAFGSFSSDLPALRPHGAPPASFS 64
           DQ V+SLRPGGG       RL  P F +++S S      G F      LRPHG   +  S
Sbjct: 4   DQPVISLRPGGGGGGPRGGRLFAPAFAVAASGS------GDF------LRPHGGGASGVS 63

Query: 65  LKAGDSRFEGRERVRFTRDQLLQLREIVEVPDDILKLKREIEVEVFGEEQSWSRGESN-- 124
            + GD   E RERVR++RDQLL LR+I +V + IL+L++EIE E+ G++QSW R +SN  
Sbjct: 64  -RIGDLHSESRERVRYSRDQLLDLRKITDVTEQILRLQQEIEAELNGDDQSWVRNDSNVQ 123

Query: 125 -----QPN-QSQNRYSEPDNRDWRGRSAQ--FSATGEDRSRETIRDRDFGGHFDSRQQEG 184
                QP  Q+QNR++E DNRDWR R+ +    A  E++S + IR+     +   RQQE 
Sbjct: 124 LQTQAQPQVQAQNRFTETDNRDWRARTEKPPAPAVQEEKSWDNIREVKEQYNASGRQQE- 183

Query: 185 NQSSRQDSNSSWTRTTSNQGGAPAALIKAEVPWSTRRGSLSEKDRVLKTVKGILNKLTPE 244
            Q +RQD +SS       Q G P ALIKA+VPWS RRG+LSEKDRVLKTVKGILNKLTPE
Sbjct: 184 -QFNRQDQSSS----QKAQVGPPPALIKADVPWSARRGNLSEKDRVLKTVKGILNKLTPE 243

Query: 245 KFDLLKGQLIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNLKLPQFPSDEP 304
           KFDLLKGQL+DSGIT+ADILKDVISLIF+KAV EPTFCPMYAQLCS+LN  LP FPS+EP
Sbjct: 244 KFDLLKGQLLDSGITTADILKDVISLIFEKAVFEPTFCPMYAQLCSELNDNLPTFPSEEP 303

Query: 305 GGREITFKRVLLNICQEAFEGADKLREEVRQMTAPEQEMERRDKERLVKLRTLGNIRLIG 364
           GG+EITFKRVLLN CQEAFEGAD LR E+  +T P+QEME+RDKER+ KLRTLGNIRLIG
Sbjct: 304 GGKEITFKRVLLNNCQEAFEGADSLRVEIASLTGPDQEMEKRDKERIFKLRTLGNIRLIG 363

Query: 365 ELLKQKMVPEKIVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENAKSRRINDM 424
           ELLKQKMVPEKIVHHIV+ELLG D K CP EE+VEAICQFFNTIGKQLDEN KSRRIND 
Sbjct: 364 ELLKQKMVPEKIVHHIVKELLGSDKKACPDEEHVEAICQFFNTIGKQLDENPKSRRINDT 423

Query: 425 YFGRLKELTTHPQLAPRLKFMVRNVLDLRANNWVPRREEIKAKTITEIHSEAERNLGLRP 484
           YF +++EL  +PQL PR KFMVR+++DLR+NNWVPRR EIKAKTI+EIH+EAE+NLGLRP
Sbjct: 424 YFVQIRELVANPQLTPRSKFMVRDLIDLRSNNWVPRRAEIKAKTISEIHTEAEKNLGLRP 483

Query: 485 GATVNIRNSRGVVSGTAGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGIDNDNWEVPR 544
           GAT N+RN R   +   G  SPGGF +NRPG GGMMPGMPG+RKMPGMPG+DNDNWEV R
Sbjct: 484 GATANMRNGR---NAPGGPLSPGGFSVNRPGTGGMMPGMPGSRKMPGMPGLDNDNWEVQR 543

Query: 545 SRPIPRGGDGSGTQPAGRGGQTPLVGKAPMLNARLLPQGTGGSISNKTSALLQGAGASTT 604
           SR +PRG       P     + P + K   +N RLLPQGTG  I    SALL G G   +
Sbjct: 544 SRSMPRGDPLRNQGPL--INKVPSINKPSPINPRLLPQGTGALIGK--SALL-GTGGPPS 603

Query: 605 RP--VNFGPGLEPSSQVQKPVTAAAVLPLAEKPQVPAASSK--QDDLEKKTLSLLEEYFS 664
           RP  +   P   P+     P  ++A       P   A+S+K     L+KKT SLLEEYF 
Sbjct: 604 RPSSLTASPTPLPAQTTASPKPSSATPASVPIPDKAASSAKVIPAGLQKKTASLLEEYFG 663

Query: 665 VRLLDEALQCVEELVSPAYHPEIVKQAISLALEKIPPCVEAVVKLLDYLLSKKVFTAGDI 724
           +R+LDEA QC+EEL SP YHPEIVK+AI+LAL+K    V+ +VKLL++L +KK F   D+
Sbjct: 664 IRILDEAQQCIEELQSPDYHPEIVKEAINLALDKGASFVDPLVKLLEHLYTKKTFKTEDL 723

Query: 725 GTGCLQYASEIDDAGIDLPKAPMNFGEIIGKLVISGGLDFQVVKEALKKVEDDRFRKDIF 784
             GCL Y S ++D GIDLPKAP  FGE++ +L++S GL F+  +  LK +ED  FRK IF
Sbjct: 724 ENGCLLYGSLLEDIGIDLPKAPTQFGEVVARLILSCGLRFEAAEGILKAMEDTFFRKAIF 783

Query: 785 DAVLKAVSSNASGQGLSDSLESDIEACRSLA 797
            +V K + ++ +GQ +  S  + ++AC SL+
Sbjct: 784 TSVTKTLGADPAGQAILSSHAAVVDACNSLS 787

BLAST of MS002322 vs. ExPASy Swiss-Prot
Match: Q6K641 (Eukaryotic translation initiation factor isoform 4G-2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0611500 PE=2 SV=1)

HSP 1 Score: 829.7 bits (2142), Expect = 2.8e-239
Identity = 461/802 (57.48%), Postives = 580/802 (72.32%), Query Frame = 0

Query: 1   MQQGDQTVLSLRPGGGRTRLLGPRFDLSSSSSSSSLAFGSFSSDLPALRPHGAPPASFSL 60
           M Q DQ V+SLRPGGG   + GPR       +     FG+ +  L  LRP G   + F+ 
Sbjct: 1   MTQADQAVISLRPGGG-GGIGGPR-------AGRLFPFGASTGSLDFLRPRGGASSGFAA 60

Query: 61  KAGDSRFEGRERVRFTRDQLLQLREIVEVPDDILKLKREIEVEVFGEEQSWSRGESNQPN 120
           K GD RFE  ERVR+TRDQL++L EI+++P++ILKLK++I++E+ GE++ W   +S+   
Sbjct: 61  KLGDLRFEPLERVRYTRDQLVELHEIIDIPENILKLKQDIDIELHGEDEPWINNDSSVQT 120

Query: 121 QSQNRYSEPDNRDWRGRSAQFSAT----GEDRSRETIRDRDFGGHFDSRQQEGNQSSRQD 180
           QS NRY+E DNRDWR R  Q   T    GE++S +  R+     +  S +Q+  Q + QD
Sbjct: 121 QSYNRYAETDNRDWRSRIEQPVQTPAIGGEEKSWDKFREAK-ESYISSGKQD--QFNNQD 180

Query: 181 SNSSWTRTTSNQGGAPAALIKAEVPWSTRRGSLSEKDRVLKTVKGILNKLTPEKFDLLKG 240
             SS     +  G AP AL+KAEVPWS +RG+LS K+RVLKTVKGILNKLTPEKFDLLKG
Sbjct: 181 KLSSQFSAKAQVGPAP-ALVKAEVPWSIQRGNLSNKERVLKTVKGILNKLTPEKFDLLKG 240

Query: 241 QLIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNLKLPQFPSDEPGGREITF 300
           QLI++GIT+ADILKDVISLIF+KAVLEPTFCPMYAQLC DLN KLP FPS+EPGG+EITF
Sbjct: 241 QLIEAGITTADILKDVISLIFEKAVLEPTFCPMYAQLCFDLNEKLPSFPSEEPGGKEITF 300

Query: 301 KRVLLNICQEAFEGADKLREEVRQMTAPEQEMERRDKERLVKLRTLGNIRLIGELLKQKM 360
           KRVLLN CQEAFEGAD LR EV ++T  +QEMERRDKERLVKLRTLGNIRL+GELLKQKM
Sbjct: 301 KRVLLNNCQEAFEGADNLRSEVNKLTGLDQEMERRDKERLVKLRTLGNIRLVGELLKQKM 360

Query: 361 VPEKIVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENAKSRRINDMYFGRLKE 420
           VPEKIVHHIVQELLG ++  CPAEENVEAICQFFNTIGKQLDEN KSRR ND+YF RLK+
Sbjct: 361 VPEKIVHHIVQELLGSESNRCPAEENVEAICQFFNTIGKQLDENPKSRRFNDVYFNRLKD 420

Query: 421 LTTHPQLAPRLKFMVRNVLDLRANNWVPRREEIKAKTITEIHSEAERNLGLRPGATVNIR 480
           LTT+ QLA RL+FM R+VLDLR+N WVPRREE+KAK I+EIH EAE NLGLRPG+T +IR
Sbjct: 421 LTTNSQLASRLRFMARDVLDLRSNQWVPRREEMKAKKISEIHREAENNLGLRPGSTASIR 480

Query: 481 NSRGVVSGTAGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGIDNDNWEVPRSRPIPRG 540
             R   +G  G  SPG F +N+PG  GM+PGMPG RKMPGMPG+ +D+WEVP SR  PR 
Sbjct: 481 TGR-TGTGGGGPLSPGAFSMNQPGIVGMLPGMPGARKMPGMPGLGSDDWEVPHSRSKPRA 540

Query: 541 GDGSGTQPAGRGGQTPLVGKAPMLNARLLPQGTGGSISNKTSALLQGAGASTTRPVNFGP 600
                  P+       L  K    N+RLLPQG+   IS KTSAL+   G     P++ G 
Sbjct: 541 DPVRNLTPS-------LANKPSPNNSRLLPQGSAALISGKTSALVGSGG-----PLSHGL 600

Query: 601 GLEPSSQVQKP---VTAAAVLPLAEKPQVPAASSKQDDLEKKTLSLLEEYFSVRLLDEAL 660
            + PS     P   + A +V P+ E+P   A      +L+KKT+SLL+EYF + LL EA 
Sbjct: 601 VVTPSQTTGPPKSLIPAPSVDPIVEQP-AAAPKPSSTELQKKTISLLKEYFHILLLHEAQ 660

Query: 661 QCVEELVSPAYHPEIVKQAISLALEKIPPCVEAVVKLLDYLLSKKVFTAGDIGTGCLQYA 720
           QC+EEL SP Y+PE+VK+AI+LAL+K    ++ +++LL++L +K VF A D+ TGCL Y+
Sbjct: 661 QCIEELKSPDYYPEVVKEAINLALDKGTNSIDPLLRLLEHLYNKNVFKATDLETGCLLYS 720

Query: 721 SEIDDAGIDLPKAPMNFGEIIGKLVISGGLDFQVVKEALKKVEDDRFRKDIFDAVLKAVS 780
           S +D+  IDLPKAP++FGE+IG+LV+S  L  +VV++ LKK+ED  FR  +F+A++K + 
Sbjct: 721 SLLDELAIDLPKAPVHFGEVIGRLVLSHCLSIEVVEDTLKKIEDSFFRAAVFEAMMKIMK 776

Query: 781 SNASGQGLSDSLESDIEACRSL 796
           +N SGQ +  S  + I+AC  L
Sbjct: 781 ANPSGQAILGSHVAKIDACSKL 776

BLAST of MS002322 vs. ExPASy Swiss-Prot
Match: Q41583 (Eukaryotic translation initiation factor isoform 4G-2 OS=Triticum aestivum OX=4565 PE=2 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 1.7e-236
Identity = 472/815 (57.91%), Postives = 574/815 (70.43%), Query Frame = 0

Query: 5   DQTVLSLRPGGG-----RTRLLGPRFDLSSSSSSSSLAFGSFSSDLPALRPHGAPPASFS 64
           DQ V+SLRPGGG       RL  P F +++S S      G F      LRPHG    +F 
Sbjct: 4   DQPVISLRPGGGGGGPRGGRLFAPAFAVAASGS------GDF------LRPHGG--GAFR 63

Query: 65  LKAGDSRFEGRERVRFTRDQLLQLREIVEVPDDILKLKREIEVEVFGEEQSWSRGESN-- 124
                      ERVR++RDQLL LR+I +V + IL+L++EIE E+ G++QSW R +SN  
Sbjct: 64  ALQDWCLHLSHERVRYSRDQLLDLRKITDVTEQILRLQQEIEAELHGDDQSWVRNDSNVQ 123

Query: 125 ------QPNQSQNRYSEPDNRDWRGRSAQ--FSATGEDRSRETIRDRDFGGHFDSRQQEG 184
                    Q+QNR++E DNRDWR R+ +    A  E++S + IR+     +   RQQE 
Sbjct: 124 LQTQTQTQVQAQNRFTETDNRDWRARTEKPPAPAVPEEKSWDNIREVKEQYNASGRQQE- 183

Query: 185 NQSSRQDSNSSWTRTTSNQGGAPAALIKAEVPWSTRRGSLSEKDRVLKTVKGILNKLTPE 244
            Q +RQD +SS       Q G P ALIKA+VPWS RRG+LSEKDRVLKTVKGILNKLTPE
Sbjct: 184 -QFNRQDQSSS----QKAQVGPPPALIKADVPWSARRGNLSEKDRVLKTVKGILNKLTPE 243

Query: 245 KFDLLKGQLIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNLKLPQFPSDEP 304
           KFDLLKG+L+DSGIT+ADILKDVISLIF+KAV EPTFCPMYAQLCS+LN  LP+FPS+EP
Sbjct: 244 KFDLLKGELLDSGITTADILKDVISLIFEKAVFEPTFCPMYAQLCSELNDNLPKFPSEEP 303

Query: 305 GGREITFKRVLLNICQEAFEGADKLREEVRQMTAPEQEMERRDKERLVKLRTLGNIRLIG 364
           GG+EITFKRVLLN CQEAFEGAD LR E+  +T P+QEME+RDKER+ KLRTLGNIRLIG
Sbjct: 304 GGKEITFKRVLLNNCQEAFEGADSLRVEIASLTGPDQEMEKRDKERIFKLRTLGNIRLIG 363

Query: 365 ELLKQKMVPEKIVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENAKSRRINDM 424
           ELLKQKMVPEKIVHHIV+ELLG D K CP EE+VEAICQFFNTIGKQLDEN KSRRIND 
Sbjct: 364 ELLKQKMVPEKIVHHIVKELLGSDKKACPDEEHVEAICQFFNTIGKQLDENPKSRRINDT 423

Query: 425 YFGRLKELTTHPQLAPRLKFMVRNVLDLRANNWVPRREEIKAKTITEIHSEAERNLGLRP 484
           YF  L+EL  +PQL PR KFMVR+++DLR+NNWVPRR EIKAKTI+EIH+EAE+NLGLRP
Sbjct: 424 YFVHLRELVANPQLTPRSKFMVRDLIDLRSNNWVPRRAEIKAKTISEIHTEAEKNLGLRP 483

Query: 485 GATVNIRNSRGVVSGTAGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGIDNDNWEVPR 544
           GAT N+RN R   +   G  SPGGF +NRPG GGMMPGMPG+RKMPGMPG+DNDNWEV R
Sbjct: 484 GATANMRNGR---NAPGGPLSPGGFSVNRPGTGGMMPGMPGSRKMPGMPGLDNDNWEVQR 543

Query: 545 SRPIPRGGDGSGTQPAGRGG----QTPLVGKAPMLNARLLPQGTGGSISNKTSALLQGAG 604
           SR +PRG       P    G    +   + K   +N RLLPQGTG  I    SALL G G
Sbjct: 544 SRSMPRG------DPLRNQGSLINKVSSINKPSPINPRLLPQGTGALIGK--SALL-GTG 603

Query: 605 ASTTRP--VNFGPGLEPSSQVQKPVTAAAVLPLAEKPQVPAASSK--QDDLEKKTLSLLE 664
              +RP  +   P   P+     P  ++A       P   A+S+K     LEKKT SLLE
Sbjct: 604 GPPSRPSSITASPTPLPAQTTASPKPSSATPASVPIPDKAASSAKVIPAGLEKKTASLLE 663

Query: 665 EYFSVRLLDEALQCVEELVSPAYHPEIVKQAISLALEKIPPCVEAVVKLLDYLLSKKVFT 724
           EYF +R+LDEA QC+EEL SP YHPEIVK+AI+LAL+K    V+ +VKLL++L +KK F 
Sbjct: 664 EYFGIRILDEAQQCIEELQSPDYHPEIVKEAINLALDKGASFVDPLVKLLEHLYTKKTFK 723

Query: 725 AGDIGTGCLQYASEIDDAGIDLPKAPMNFGEIIGKLVISGGLDFQVVKEALKKVEDDRFR 784
             D+  GCL Y S ++D GIDLPKAP  FGE+I +L++S GL F+ V+  LK +ED  FR
Sbjct: 724 TEDLENGCLLYGSLLEDIGIDLPKAPTQFGEVIARLILSCGLRFEAVEGILKAMEDTFFR 783

Query: 785 KDIFDAVLKAVSSNASGQGLSDSLESDIEACRSLA 797
           K IF +V K + ++ +GQ +  S  + ++AC SL+
Sbjct: 784 KAIFTSVTKTLEADPAGQAILSSHAAVVDACNSLS 786

BLAST of MS002322 vs. ExPASy TrEMBL
Match: A0A6J1C2U4 (eukaryotic translation initiation factor OS=Momordica charantia OX=3673 GN=LOC111007430 PE=4 SV=1)

HSP 1 Score: 1520.8 bits (3936), Expect = 0.0e+00
Identity = 788/797 (98.87%), Postives = 789/797 (99.00%), Query Frame = 0

Query: 1   MQQGDQTVLSLRPGGGRTRLLGPRFDLSSSSSSSSLAFGSFSSDLPALRPHGAPPASFSL 60
           MQQGDQTVLSLRPGGGRTRLLGPRFDLSSSSSSSSLAFGSFSSDLPALRPHGAPPASFSL
Sbjct: 1   MQQGDQTVLSLRPGGGRTRLLGPRFDLSSSSSSSSLAFGSFSSDLPALRPHGAPPASFSL 60

Query: 61  KAGDSRFEGRERVRFTRDQLLQLREIVEVPDDILKLKREIEVEVFGEEQSWSRGESNQPN 120
           KAGDSRFEGRERVRFTRDQLLQLREIVEVPDDILKLKREIEVEVFGEEQSWSRGESNQPN
Sbjct: 61  KAGDSRFEGRERVRFTRDQLLQLREIVEVPDDILKLKREIEVEVFGEEQSWSRGESNQPN 120

Query: 121 QSQNRYSEPDNRDWRGRSAQFSATGEDRSRETIRDRDFGGHFDSRQQEGNQSSRQDSNSS 180
           QSQNRYSEPDNRDWRGRSAQFSATGEDRSRETIRDRDFGGHFDSRQQEGNQSSRQDSNSS
Sbjct: 121 QSQNRYSEPDNRDWRGRSAQFSATGEDRSRETIRDRDFGGHFDSRQQEGNQSSRQDSNSS 180

Query: 181 WTRTTSNQGGAPAALIKAEVPWSTRRGSLSEKDRVLKTVKGILNKLTPEKFDLLKGQLID 240
           WTRTTSNQGGAPAALIKAEVPWS RRGSLSEKDRVLKTVKGILNKLTPEKFDLLKGQLID
Sbjct: 181 WTRTTSNQGGAPAALIKAEVPWSARRGSLSEKDRVLKTVKGILNKLTPEKFDLLKGQLID 240

Query: 241 SGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNLKLPQFPSDEPGGREITFKRVL 300
           SGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNLKLPQFPSDEPGGREITFKRVL
Sbjct: 241 SGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNLKLPQFPSDEPGGREITFKRVL 300

Query: 301 LNICQEAFEGADKLREEVRQMTAPEQEMERRDKERLVKLRTLGNIRLIGELLKQKMVPEK 360
           LNICQEAFEGADKLREEVRQMTAPEQEMERRDKERLVKLRTLGNIRLIGELLKQKMVPEK
Sbjct: 301 LNICQEAFEGADKLREEVRQMTAPEQEMERRDKERLVKLRTLGNIRLIGELLKQKMVPEK 360

Query: 361 IVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENAKSRRINDMYFGRLKELTTH 420
           IVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENAKSRRINDMYFGRLKELTTH
Sbjct: 361 IVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENAKSRRINDMYFGRLKELTTH 420

Query: 421 PQLAPRLKFMVRNVLDLRANNWVPRREEIKAKTITEIHSEAERNLGLRPGATVNIRNSRG 480
           PQLAPRLKFMVRNVLDLRANNWVP      AKTITEIHSEAERNLGLRPGATVNIRNSRG
Sbjct: 421 PQLAPRLKFMVRNVLDLRANNWVPXXXXXXAKTITEIHSEAERNLGLRPGATVNIRNSRG 480

Query: 481 VVSGTAGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGIDNDNWEVPRSRPIPRGGDGS 540
           VVSG+AGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGIDNDNWEVPRSRPIPRGGDGS
Sbjct: 481 VVSGSAGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGIDNDNWEVPRSRPIPRGGDGS 540

Query: 541 GTQPAGRGGQTPLVGKAPMLNARLLPQGTGGSISNKTSALLQGAGASTTRPVNFGPGLEP 600
           GTQPAGRGGQTPLVGKAPMLNARLLPQGTGGSISNKTSALLQGAGASTTRPVNFGPGLEP
Sbjct: 541 GTQPAGRGGQTPLVGKAPMLNARLLPQGTGGSISNKTSALLQGAGASTTRPVNFGPGLEP 600

Query: 601 SSQVQKPVTAAAVLPLAEKPQVPAASSKQDDLEKKTLSLLEEYFSVRLLDEALQCVEELV 660
           SSQVQKPVTAAAVLPLAEKPQVPAASSKQDDLEKKTLSLLEEYFSVRLLDEALQCVEELV
Sbjct: 601 SSQVQKPVTAAAVLPLAEKPQVPAASSKQDDLEKKTLSLLEEYFSVRLLDEALQCVEELV 660

Query: 661 SPAYHPEIVKQAISLALEKIPPCVEAVVKLLDYLLSKKVFTAGDIGTGCLQYASEIDDAG 720
           SPAYHPEIVKQAISLALEKIPPCVEAVVKLLDYLLSKKVFTAGDIGTGCLQYASEIDD G
Sbjct: 661 SPAYHPEIVKQAISLALEKIPPCVEAVVKLLDYLLSKKVFTAGDIGTGCLQYASEIDDVG 720

Query: 721 IDLPKAPMNFGEIIGKLVISGGLDFQVVKEALKKVEDDRFRKDIFDAVLKAVSSNASGQG 780
           IDLPKAPMNFGEIIGKLVISGGLDFQVVKEALKKVEDDRFRKDIFDAVLKAVSSNASGQG
Sbjct: 721 IDLPKAPMNFGEIIGKLVISGGLDFQVVKEALKKVEDDRFRKDIFDAVLKAVSSNASGQG 780

Query: 781 LSDSLESDIEACRSLAY 798
           LSDSLESDIEACRSLAY
Sbjct: 781 LSDSLESDIEACRSLAY 797

BLAST of MS002322 vs. ExPASy TrEMBL
Match: A0A6J1E268 (eukaryotic translation initiation factor OS=Cucurbita moschata OX=3662 GN=LOC111429890 PE=4 SV=1)

HSP 1 Score: 1352.4 bits (3499), Expect = 0.0e+00
Identity = 697/797 (87.45%), Postives = 739/797 (92.72%), Query Frame = 0

Query: 1   MQQGDQTVLSLRPGGGRTRLLGPRFDLSSSSSSSSLAFGSFSSDLPALRPHGAPPASFSL 60
           MQQGDQTVLSLRPGGGR RLLGPRF+L SSSSSSS AFGSFSSDLP LRPHGA  +SFS+
Sbjct: 1   MQQGDQTVLSLRPGGGRPRLLGPRFEL-SSSSSSSFAFGSFSSDLPTLRPHGASSSSFSV 60

Query: 61  KAGDSRFEGRERVRFTRDQLLQLREIVEVPDDILKLKREIEVEVFGEEQSWSRGESNQPN 120
           KAGDSRFEGRERVR+TRDQLLQL+E+VEVPDDILK+KR+IEVE+FGEEQSWSRGESNQ N
Sbjct: 61  KAGDSRFEGRERVRYTRDQLLQLQEVVEVPDDILKIKRDIEVEIFGEEQSWSRGESNQLN 120

Query: 121 QSQNRYSEPDNRDWRGRSAQFSATGEDRSRETIRDRDFGGHFDSRQQEGNQSSRQDSNSS 180
            SQNRYSEPDNRDWRGRSAQFSA+GEDRSRETIRDRDFGGHFDSRQQ+ NQ +RQD +SS
Sbjct: 121 PSQNRYSEPDNRDWRGRSAQFSASGEDRSRETIRDRDFGGHFDSRQQDANQLNRQD-HSS 180

Query: 181 WTRTTSNQGGAPAALIKAEVPWSTRRGSLSEKDRVLKTVKGILNKLTPEKFDLLKGQLID 240
           WTRTT NQGG P ALIKAEVPWS RRG+LS+KDRVLKTVKGILNKLTPEKFDLLKGQLID
Sbjct: 181 WTRTTPNQGGGPPALIKAEVPWSARRGTLSDKDRVLKTVKGILNKLTPEKFDLLKGQLID 240

Query: 241 SGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNLKLPQFPSDEPGGREITFKRVL 300
           SGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLN KLPQFPSDEPGGRE+TFKRVL
Sbjct: 241 SGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNQKLPQFPSDEPGGREVTFKRVL 300

Query: 301 LNICQEAFEGADKLREEVRQMTAPEQEMERRDKERLVKLRTLGNIRLIGELLKQKMVPEK 360
           LNICQEAFEGADKLREE+RQMTAPEQE ERRDK+RL+KLRTLGNIRLIGELLKQKMVPEK
Sbjct: 301 LNICQEAFEGADKLREELRQMTAPEQESERRDKDRLLKLRTLGNIRLIGELLKQKMVPEK 360

Query: 361 IVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENAKSRRINDMYFGRLKELTTH 420
           IVHHIVQELLGPDNK+CPAEENVEAICQFFNTIGKQLDEN KSRRINDMYFGRLKELTTH
Sbjct: 361 IVHHIVQELLGPDNKSCPAEENVEAICQFFNTIGKQLDENTKSRRINDMYFGRLKELTTH 420

Query: 421 PQLAPRLKFMVRNVLDLRANNWVPRREEIKAKTITEIHSEAERNLGLRPGATVNIRNSRG 480
            QLAPRLKFMVR+VLDLRANNWVPRREE+KAKTITEIH+EAE+NLGLRPGATVN+RN+RG
Sbjct: 421 TQLAPRLKFMVRDVLDLRANNWVPRREEVKAKTITEIHTEAEKNLGLRPGATVNMRNNRG 480

Query: 481 VVSGTAGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGIDNDNWEVPRSRPIPRGGDGS 540
           VVSG+ GNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPG+DNDNWEV R+RPIPRG DGS
Sbjct: 481 VVSGSPGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGMDNDNWEVHRTRPIPRGADGS 540

Query: 541 GTQPAGRGGQTPLVGKAPMLNARLLPQGTGGSISNKTSALLQGAGASTTRPVNFGPGLEP 600
           G       G    VGK   LNARLLPQGTGG ISNK SALLQGAGASTTRPVNFG GLEP
Sbjct: 541 GR------GSASFVGKGSALNARLLPQGTGGLISNKNSALLQGAGASTTRPVNFGHGLEP 600

Query: 601 SSQVQKPVTAAAVLPLAEKPQVPAASSKQDDLEKKTLSLLEEYFSVRLLDEALQCVEELV 660
           S QV KP+ AA V P AE+PQVPAAS KQDDL KKT SLLEEYFS+R+LDEALQCVEEL+
Sbjct: 601 SGQVHKPIAAATVPPPAERPQVPAASLKQDDLRKKTHSLLEEYFSIRILDEALQCVEELM 660

Query: 661 SPAYHPEIVKQAISLALEKIPPCVEAVVKLLDYLLSKKVFTAGDIGTGCLQYASEIDDAG 720
           SPAYHPE+VK+AISLALEK PPCVEAVVK LDYLLS+KVFTA DIGTGCL YAS +DDAG
Sbjct: 661 SPAYHPEVVKEAISLALEKSPPCVEAVVKFLDYLLSRKVFTASDIGTGCLLYASMLDDAG 720

Query: 721 IDLPKAPMNFGEIIGKLVISGGLDFQVVKEALKKVEDDRFRKDIFDAVLKAVSSNASGQG 780
           IDLPKAP+NFGEIIGKLVISGGLDFQVVKEA+KKVEDDRFRKDIFDAVLK +SSN SGQG
Sbjct: 721 IDLPKAPINFGEIIGKLVISGGLDFQVVKEAIKKVEDDRFRKDIFDAVLKTISSNVSGQG 780

Query: 781 LSDSLESDIEACRSLAY 798
           L DSL SDIE CR+LAY
Sbjct: 781 LLDSLASDIEGCRNLAY 789

BLAST of MS002322 vs. ExPASy TrEMBL
Match: A0A6J1JLW2 (eukaryotic translation initiation factor OS=Cucurbita maxima OX=3661 GN=LOC111485757 PE=4 SV=1)

HSP 1 Score: 1347.4 bits (3486), Expect = 0.0e+00
Identity = 691/800 (86.38%), Postives = 738/800 (92.25%), Query Frame = 0

Query: 1   MQQGDQTVLSLRPGGGRTRLLGPRFDL---SSSSSSSSLAFGSFSSDLPALRPHGAPPAS 60
           MQQGDQTVLSLRPGGGR RLLGPRF+L   SSSSSSSS AFGSFSSDLP+LRPHGA  +S
Sbjct: 1   MQQGDQTVLSLRPGGGRPRLLGPRFELSSSSSSSSSSSFAFGSFSSDLPSLRPHGASSSS 60

Query: 61  FSLKAGDSRFEGRERVRFTRDQLLQLREIVEVPDDILKLKREIEVEVFGEEQSWSRGESN 120
           FS+KAGDSRFEGRERVR+TRDQLLQL+E VEVPDDILK+KR+IEVE+FGEEQSWSRGESN
Sbjct: 61  FSVKAGDSRFEGRERVRYTRDQLLQLQEAVEVPDDILKIKRDIEVEIFGEEQSWSRGESN 120

Query: 121 QPNQSQNRYSEPDNRDWRGRSAQFSATGEDRSRETIRDRDFGGHFDSRQQEGNQSSRQDS 180
           Q N SQNRYSEPDNRDWRGRSAQFS++GEDRSRETIRDRDFGGHFDSRQQ+ NQ +RQD 
Sbjct: 121 QLNLSQNRYSEPDNRDWRGRSAQFSSSGEDRSRETIRDRDFGGHFDSRQQDANQLNRQDP 180

Query: 181 NSSWTRTTSNQGGAPAALIKAEVPWSTRRGSLSEKDRVLKTVKGILNKLTPEKFDLLKGQ 240
           +SSWTRTT NQGG P ALIKAEVPWS RRG+LS+KDRVLKTVKGILNKLTPEKFDLLKGQ
Sbjct: 181 HSSWTRTTPNQGGGPPALIKAEVPWSARRGTLSDKDRVLKTVKGILNKLTPEKFDLLKGQ 240

Query: 241 LIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNLKLPQFPSDEPGGREITFK 300
           LIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLN KLPQFPSDEPGGRE+TFK
Sbjct: 241 LIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNQKLPQFPSDEPGGREVTFK 300

Query: 301 RVLLNICQEAFEGADKLREEVRQMTAPEQEMERRDKERLVKLRTLGNIRLIGELLKQKMV 360
           RVLLNICQEAFEGADKLREE+RQMTAPEQE ERRDK+RL+KLRTLGNIRLIGELLKQKMV
Sbjct: 301 RVLLNICQEAFEGADKLREELRQMTAPEQESERRDKDRLLKLRTLGNIRLIGELLKQKMV 360

Query: 361 PEKIVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENAKSRRINDMYFGRLKEL 420
           PEKIVHHIVQELLGPDNK+CPAEENVEAICQFFNTIGKQLDEN KSRRINDMYFGRLKEL
Sbjct: 361 PEKIVHHIVQELLGPDNKSCPAEENVEAICQFFNTIGKQLDENTKSRRINDMYFGRLKEL 420

Query: 421 TTHPQLAPRLKFMVRNVLDLRANNWVPRREEIKAKTITEIHSEAERNLGLRPGATVNIRN 480
           TTH QLAPRLKFMVR+VL+LRANNWVPRREE+KAKTITEIH+EAE+NLGLRPGATVN+RN
Sbjct: 421 TTHTQLAPRLKFMVRDVLELRANNWVPRREEVKAKTITEIHTEAEKNLGLRPGATVNMRN 480

Query: 481 SRGVVSGTAGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGIDNDNWEVPRSRPIPRGG 540
           +RGVVSG+ GNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPG+DNDNWEV R+RPIPRG 
Sbjct: 481 NRGVVSGSPGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGMDNDNWEVHRTRPIPRGA 540

Query: 541 DGSGTQPAGRGGQTPLVGKAPMLNARLLPQGTGGSISNKTSALLQGAGASTTRPVNFGPG 600
           DGSG       G     GK   LNARLLPQGTGG ISNK SALLQGAGA+TTRPVNFG G
Sbjct: 541 DGSGR------GSASFAGKGSALNARLLPQGTGGLISNKNSALLQGAGATTTRPVNFGHG 600

Query: 601 LEPSSQVQKPVTAAAVLPLAEKPQVPAASSKQDDLEKKTLSLLEEYFSVRLLDEALQCVE 660
           LEPS QV KP+ AA V P AE+PQ+PAAS KQDDL KKT SLLEEYFS+R+LDEALQCVE
Sbjct: 601 LEPSGQVHKPIAAATVPPPAERPQIPAASLKQDDLRKKTHSLLEEYFSIRILDEALQCVE 660

Query: 661 ELVSPAYHPEIVKQAISLALEKIPPCVEAVVKLLDYLLSKKVFTAGDIGTGCLQYASEID 720
           EL++P YHPE+VK+AISLALEK PPCVEAVVK LDYLLS+KVFTA D+GTGCL YAS +D
Sbjct: 661 ELMTPTYHPEVVKEAISLALEKSPPCVEAVVKFLDYLLSRKVFTASDVGTGCLLYASMLD 720

Query: 721 DAGIDLPKAPMNFGEIIGKLVISGGLDFQVVKEALKKVEDDRFRKDIFDAVLKAVSSNAS 780
           DAGIDLPKAP+NFGEIIGKLVISGGLDFQVVKEA+KKVEDDRFRKDIFDAVLK +SSN S
Sbjct: 721 DAGIDLPKAPINFGEIIGKLVISGGLDFQVVKEAIKKVEDDRFRKDIFDAVLKTISSNVS 780

Query: 781 GQGLSDSLESDIEACRSLAY 798
           GQGL DSL SDIE CRSLAY
Sbjct: 781 GQGLLDSLASDIEGCRSLAY 794

BLAST of MS002322 vs. ExPASy TrEMBL
Match: A0A5A7T0H9 (Eukaryotic translation initiation factor OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold57G001760 PE=4 SV=1)

HSP 1 Score: 1347.0 bits (3485), Expect = 0.0e+00
Identity = 705/803 (87.80%), Postives = 738/803 (91.91%), Query Frame = 0

Query: 1   MQQGDQTVLSLRPGGGRTRLLGPRFDL----SSSSSSSSLAFGSFSSDLPALRPHGAPPA 60
           MQ+GDQTVLSLRPGGGR RLLGPRFDL    SSSSSSSS AFGSFSSDLP LRPH A  A
Sbjct: 1   MQKGDQTVLSLRPGGGRPRLLGPRFDLSSSSSSSSSSSSFAFGSFSSDLPTLRPHAASSA 60

Query: 61  SFSLKAGDSRFEGRERVRFTRDQLLQLREIVEVPDDILKLKREIEVEVFGEEQSWSRGES 120
           SFS+K GDSRFEGRERVR+TRDQLLQLRE VEVPDDILK+KREI+VE+FGEEQSWSRGES
Sbjct: 61  SFSVKGGDSRFEGRERVRYTRDQLLQLREGVEVPDDILKIKREIDVEIFGEEQSWSRGES 120

Query: 121 NQPNQSQNRYSEPDNRDWRGRSAQFSATGEDRSRETIRDRDFGGHFDSRQQEGNQSSRQD 180
           NQ NQSQNRYSEPDNRDWRGRSAQFSA+GE+RSRET RDRDFGGHFDSRQQ+ N  +RQD
Sbjct: 121 NQLNQSQNRYSEPDNRDWRGRSAQFSASGEERSRETNRDRDFGGHFDSRQQDANNLNRQD 180

Query: 181 SNSSWTRTTSNQGGAPAALIKAEVPWSTRRGSLSEKDRVLKTVKGILNKLTPEKFDLLKG 240
           S+SSWTRTTSNQGGAP ALIKAEVPWS RRG+LS+KDRVLKTVKGILNKLTPEKFDLLKG
Sbjct: 181 SHSSWTRTTSNQGGAPPALIKAEVPWSARRGNLSDKDRVLKTVKGILNKLTPEKFDLLKG 240

Query: 241 QLIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNLKLPQFPSDEPGGREITF 300
           QLIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLN KLPQFPSDEPGGREITF
Sbjct: 241 QLIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNQKLPQFPSDEPGGREITF 300

Query: 301 KRVLLNICQEAFEGADKLREEVRQMTAPEQEMERRDKERLVKLRTLGNIRLIGELLKQKM 360
           KRVLLNICQEAFEGADKLREEVRQMTAPEQE ERRDK+RLVKLRTLGNIRLIGELLKQKM
Sbjct: 301 KRVLLNICQEAFEGADKLREEVRQMTAPEQESERRDKDRLVKLRTLGNIRLIGELLKQKM 360

Query: 361 VPEKIVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENAKSRRINDMYFGRLKE 420
           VPEKIVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDEN KSRRINDMYFGRLKE
Sbjct: 361 VPEKIVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENTKSRRINDMYFGRLKE 420

Query: 421 LTTHPQLAPRLKFMVRNVLDLRANNWVPRREEIKAKTITEIHSEAERNLGLRPGATVNIR 480
           LTTH QLAPRLKFMVR+VLDLRANNWVPRREE+KAKTITEIHSEAE+NLGLRPGATVNIR
Sbjct: 421 LTTHTQLAPRLKFMVRDVLDLRANNWVPRREEVKAKTITEIHSEAEKNLGLRPGATVNIR 480

Query: 481 NSRGVVSGTAGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGIDNDNWEVPRSRPIPRG 540
           NSR V SG+AGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPG+DNDNWEVPR+R I RG
Sbjct: 481 NSR-VASGSAGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGMDNDNWEVPRARSITRG 540

Query: 541 GDGSGTQPAGRGGQTPLVGKAPMLNARLLPQGTGGSISNKTSALL--QGAGASTTRPVNF 600
            DGSG Q AGRG   PLVGKAP LNARLLPQGTGG ISNKTSALL  QGAG STTRPVNF
Sbjct: 541 ADGSGIQSAGRG--QPLVGKAPTLNARLLPQGTGGLISNKTSALLQGQGAGGSTTRPVNF 600

Query: 601 GPGLEPSSQVQKPVTAAAVLPLAEKPQVPAASSKQDDLEKKTLSLLEEYFSVRLLDEALQ 660
           G GLEPS QV KPVTA  VL  AE+ Q P  S KQDDL+KKTLSLLEEYFS+RLLDEALQ
Sbjct: 601 GHGLEPSGQVHKPVTAPTVLSPAERQQAPVTSLKQDDLKKKTLSLLEEYFSIRLLDEALQ 660

Query: 661 CVEELVSPAYHPEIVKQAISLALEKIPPCVEAVVKLLDYLLSKKVFTAGDIGTGCLQYAS 720
           CVEEL+SPAYHPE+VK+AISLALEK PPCVEAVVK LDYLLSKKVFT GDI TGCL YAS
Sbjct: 661 CVEELMSPAYHPEVVKEAISLALEKSPPCVEAVVKFLDYLLSKKVFTTGDIETGCLGYAS 720

Query: 721 EIDDAGIDLPKAPMNFGEIIGKLVISGGLDFQVVKEALKKVEDDRFRKDIFDAVLKAVSS 780
            + DAGIDLPKAP+NFGEIIGKLV+S  LDFQVVKEA+KKVEDDRFRKD+FDAVL+ + S
Sbjct: 721 MLHDAGIDLPKAPINFGEIIGKLVLSRCLDFQVVKEAIKKVEDDRFRKDVFDAVLQTIRS 780

Query: 781 NASGQGLSDSLESDIEACRSLAY 798
           N SGQGL DSL SDIE CRSLAY
Sbjct: 781 NMSGQGLLDSLASDIEECRSLAY 800

BLAST of MS002322 vs. ExPASy TrEMBL
Match: A0A1S3B918 (eukaryotic translation initiation factor OS=Cucumis melo OX=3656 GN=LOC103487340 PE=4 SV=1)

HSP 1 Score: 1347.0 bits (3485), Expect = 0.0e+00
Identity = 705/803 (87.80%), Postives = 738/803 (91.91%), Query Frame = 0

Query: 1   MQQGDQTVLSLRPGGGRTRLLGPRFDL----SSSSSSSSLAFGSFSSDLPALRPHGAPPA 60
           MQ+GDQTVLSLRPGGGR RLLGPRFDL    SSSSSSSS AFGSFSSDLP LRPH A  A
Sbjct: 1   MQKGDQTVLSLRPGGGRPRLLGPRFDLSSSSSSSSSSSSFAFGSFSSDLPTLRPHAASSA 60

Query: 61  SFSLKAGDSRFEGRERVRFTRDQLLQLREIVEVPDDILKLKREIEVEVFGEEQSWSRGES 120
           SFS+K GDSRFEGRERVR+TRDQLLQLRE VEVPDDILK+KREI+VE+FGEEQSWSRGES
Sbjct: 61  SFSVKGGDSRFEGRERVRYTRDQLLQLREGVEVPDDILKIKREIDVEIFGEEQSWSRGES 120

Query: 121 NQPNQSQNRYSEPDNRDWRGRSAQFSATGEDRSRETIRDRDFGGHFDSRQQEGNQSSRQD 180
           NQ NQSQNRYSEPDNRDWRGRSAQFSA+GE+RSRET RDRDFGGHFDSRQQ+ N  +RQD
Sbjct: 121 NQLNQSQNRYSEPDNRDWRGRSAQFSASGEERSRETNRDRDFGGHFDSRQQDANNLNRQD 180

Query: 181 SNSSWTRTTSNQGGAPAALIKAEVPWSTRRGSLSEKDRVLKTVKGILNKLTPEKFDLLKG 240
           S+SSWTRTTSNQGGAP ALIKAEVPWS RRG+LS+KDRVLKTVKGILNKLTPEKFDLLKG
Sbjct: 181 SHSSWTRTTSNQGGAPPALIKAEVPWSARRGNLSDKDRVLKTVKGILNKLTPEKFDLLKG 240

Query: 241 QLIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNLKLPQFPSDEPGGREITF 300
           QLIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLN KLPQFPSDEPGGREITF
Sbjct: 241 QLIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNQKLPQFPSDEPGGREITF 300

Query: 301 KRVLLNICQEAFEGADKLREEVRQMTAPEQEMERRDKERLVKLRTLGNIRLIGELLKQKM 360
           KRVLLNICQEAFEGADKLREEVRQMTAPEQE ERRDK+RLVKLRTLGNIRLIGELLKQKM
Sbjct: 301 KRVLLNICQEAFEGADKLREEVRQMTAPEQESERRDKDRLVKLRTLGNIRLIGELLKQKM 360

Query: 361 VPEKIVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENAKSRRINDMYFGRLKE 420
           VPEKIVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDEN KSRRINDMYFGRLKE
Sbjct: 361 VPEKIVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENTKSRRINDMYFGRLKE 420

Query: 421 LTTHPQLAPRLKFMVRNVLDLRANNWVPRREEIKAKTITEIHSEAERNLGLRPGATVNIR 480
           LTTH QLAPRLKFMVR+VLDLRANNWVPRREE+KAKTITEIHSEAE+NLGLRPGATVNIR
Sbjct: 421 LTTHTQLAPRLKFMVRDVLDLRANNWVPRREEVKAKTITEIHSEAEKNLGLRPGATVNIR 480

Query: 481 NSRGVVSGTAGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGIDNDNWEVPRSRPIPRG 540
           NSR V SG+AGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPG+DNDNWEVPR+R I RG
Sbjct: 481 NSR-VASGSAGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGMDNDNWEVPRARSITRG 540

Query: 541 GDGSGTQPAGRGGQTPLVGKAPMLNARLLPQGTGGSISNKTSALL--QGAGASTTRPVNF 600
            DGSG Q AGRG   PLVGKAP LNARLLPQGTGG ISNKTSALL  QGAG STTRPVNF
Sbjct: 541 ADGSGIQSAGRG--QPLVGKAPTLNARLLPQGTGGLISNKTSALLQGQGAGGSTTRPVNF 600

Query: 601 GPGLEPSSQVQKPVTAAAVLPLAEKPQVPAASSKQDDLEKKTLSLLEEYFSVRLLDEALQ 660
           G GLEPS QV KPVTA  VL  AE+ Q P  S KQDDL+KKTLSLLEEYFS+RLLDEALQ
Sbjct: 601 GHGLEPSGQVHKPVTAPTVLSPAERQQAPVTSLKQDDLKKKTLSLLEEYFSIRLLDEALQ 660

Query: 661 CVEELVSPAYHPEIVKQAISLALEKIPPCVEAVVKLLDYLLSKKVFTAGDIGTGCLQYAS 720
           CVEEL+SPAYHPE+VK+AISLALEK PPCVEAVVK LDYLLSKKVFT GDI TGCL YAS
Sbjct: 661 CVEELMSPAYHPEVVKEAISLALEKSPPCVEAVVKFLDYLLSKKVFTTGDIETGCLGYAS 720

Query: 721 EIDDAGIDLPKAPMNFGEIIGKLVISGGLDFQVVKEALKKVEDDRFRKDIFDAVLKAVSS 780
            + DAGIDLPKAP+NFGEIIGKLV+S  LDFQVVKEA+KKVEDDRFRKD+FDAVL+ + S
Sbjct: 721 MLHDAGIDLPKAPINFGEIIGKLVLSRCLDFQVVKEAIKKVEDDRFRKDVFDAVLQTIRS 780

Query: 781 NASGQGLSDSLESDIEACRSLAY 798
           N SGQGL DSL SDIE CRSLAY
Sbjct: 781 NMSGQGLLDSLASDIEECRSLAY 800

BLAST of MS002322 vs. TAIR 10
Match: AT5G57870.2 (MIF4G domain-containing protein / MA3 domain-containing protein )

HSP 1 Score: 932.9 bits (2410), Expect = 1.7e-271
Identity = 516/803 (64.26%), Postives = 627/803 (78.08%), Query Frame = 0

Query: 1   MQQGDQTVLSLRPGGGR-TRLLGPRFDLSSSSSSSSLAFGSF-SSDLPALRPH-GAPPAS 60
           MQQGDQTVLSLRPGGGR  RL G      SSSSSSSL+FGS  SSDLP LRPH GAP +S
Sbjct: 1   MQQGDQTVLSLRPGGGRGNRLFG------SSSSSSSLSFGSLSSSDLPLLRPHGGAPASS 60

Query: 61  FSLKAGDSRFEGRERVRFTRDQLLQLREIVEVPDDILKLKREIEVEVFGEEQSWSRGESN 120
           F  K GDSRF+GRERV++TR+QLL+L+E  ++ D+IL+++RE   E+FGEE +W+RGES 
Sbjct: 61  FPFKGGDSRFDGRERVKYTREQLLELKETTQLSDEILRVQRETAAELFGEEGTWARGESV 120

Query: 121 QPNQSQNRYSEPDNRDWRGRSAQFSATGEDRSRETIRDRDFGGHFDSRQQEGNQSSRQDS 180
            P QS +R+SEPD+RDWR RS Q   +GE+RS + +R+       DSR  E +Q +RQD 
Sbjct: 121 VPVQSASRFSEPDSRDWRSRSTQPPPSGEERSWDNLREAK-----DSRYVEASQYNRQDQ 180

Query: 181 -NSSWTRT--TSNQGGAPA-ALIKAEVPWSTRRGSLSEKDRVLKTVKGILNKLTPEKFDL 240
            NS ++R   +SNQGG PA  L+KAEVPWS RRG+LSE DRVLKTVKGILNKLTPEK+DL
Sbjct: 181 PNSQFSRANISSNQGGGPAPVLVKAEVPWSARRGNLSENDRVLKTVKGILNKLTPEKYDL 240

Query: 241 LKGQLIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNLKLPQFPSDEPGGRE 300
           LKGQLI+SGITSADILK VI+LIFDKAVLEPTFCPMYA+LCSD+N +LP FP  EPG +E
Sbjct: 241 LKGQLIESGITSADILKGVITLIFDKAVLEPTFCPMYAKLCSDINDQLPTFPPAEPGDKE 300

Query: 301 ITFKRVLLNICQEAFEGADKLREEVRQMTAPEQEMERRDKERLVKLRTLGNIRLIGELLK 360
           ITFKRVLLNICQEAFEGA +LREE+RQM+AP+QE ER DKE+L+KL+TLGNIRLIGELLK
Sbjct: 301 ITFKRVLLNICQEAFEGASQLREELRQMSAPDQEAERNDKEKLLKLKTLGNIRLIGELLK 360

Query: 361 QKMVPEKIVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENAKSRRINDMYFGR 420
           QKMVPEKIVHHIVQELLG D K CPAEENVEAIC FF TIGKQLD N KS+RIND+YF R
Sbjct: 361 QKMVPEKIVHHIVQELLGADEKVCPAEENVEAICHFFKTIGKQLDGNVKSKRINDVYFKR 420

Query: 421 LKELTTHPQLAPRLKFMVRNVLDLRANNWVPRREEIKAKTITEIHSEAERNLGLRPGATV 480
           L+ L+ +PQL  RL+FMV+N++D+R+N WVPRREE+KA+TITEIH+EAE+NLGLRPGAT 
Sbjct: 421 LQALSKNPQLELRLRFMVQNIIDMRSNGWVPRREEMKARTITEIHTEAEKNLGLRPGATA 480

Query: 481 NIRNSRGVVSGTAGNTSPGG-FPINRPGFGGMMPGMPGTRKMPGMPGIDNDNWEVPRSRP 540
           N+R  RG+VS + G  SPG  +P  RPG GG+MPGMPGTR+MPGMPG+DNDNWEVPR+R 
Sbjct: 481 NMR--RGMVS-SGGPVSPGPVYPGGRPGAGGLMPGMPGTRRMPGMPGVDNDNWEVPRTRS 540

Query: 541 IPRGGDGSGTQPAGRGGQTPLVGKAPMLNARLLPQGTGGSISNKTSALLQGAGASTTRPV 600
           + R  DG G         +P V K+  +N RLLPQG+ G +S KTSALLQG+G S +RPV
Sbjct: 541 MSR-RDGPGPL------HSPAVSKSASMNTRLLPQGSSGIMSGKTSALLQGSG-SVSRPV 600

Query: 601 NFGPGLEPSSQVQKPVTAAAVLPLAEKPQVPAASSKQDDLEKKTLSLLEEYFSVRLLDEA 660
                 E  +Q   P+T    +   EKPQ       ++ L++KT SLLEEYF+VRLL EA
Sbjct: 601 TV--SAERPAQSVAPLTVPVPV---EKPQPSGPKLSEEVLQRKTKSLLEEYFNVRLLGEA 660

Query: 661 LQCVEELVSPAYHPEIVKQAISLALEKIPPCVEAVVKLLDYLLSKKVFTAGDIGTGCLQY 720
           LQCVEEL  P+YHPE VK+AISL+LEK PP VE +  LL+YLLSKKV    D+ TG L Y
Sbjct: 661 LQCVEELGLPSYHPEFVKEAISLSLEKSPPVVEPIATLLEYLLSKKVVAPKDLETGFLLY 720

Query: 721 ASEIDDAGIDLPKAPMNFGEIIGKLVISGGLDFQVVKEALKKVEDDRFRKDIFDAVLKAV 780
            + +DD GIDLPKAP NFGEI+GKL+++GG+DF++V+E + K+EDDRF+K + DA ++ V
Sbjct: 721 GAMLDDIGIDLPKAPNNFGEIVGKLILAGGVDFKLVREIIGKMEDDRFQKMVVDAAVRIV 776

Query: 781 SSNASGQGLSDSLESDIEACRSL 796
            S+  G+ L  S  +DIEACR+L
Sbjct: 781 ESSEQGKSLLASQAADIEACRNL 776

BLAST of MS002322 vs. TAIR 10
Match: AT5G57870.1 (MIF4G domain-containing protein / MA3 domain-containing protein )

HSP 1 Score: 927.5 bits (2396), Expect = 7.0e-270
Identity = 516/807 (63.94%), Postives = 627/807 (77.70%), Query Frame = 0

Query: 1   MQQGDQTVLSLRPGGGR-TRLLGPRFDLSSSSSSSSLAFGSF-SSDLPALRPH-GAPPAS 60
           MQQGDQTVLSLRPGGGR  RL G      SSSSSSSL+FGS  SSDLP LRPH GAP +S
Sbjct: 1   MQQGDQTVLSLRPGGGRGNRLFG------SSSSSSSLSFGSLSSSDLPLLRPHGGAPASS 60

Query: 61  FSLKAGDSRFEGRERVRFTRDQLLQLREIVEVPDDILKLKREIEVEVFGEEQSWSRGESN 120
           F  K GDSRF+GRERV++TR+QLL+L+E  ++ D+IL+++RE   E+FGEE +W+RGES 
Sbjct: 61  FPFKGGDSRFDGRERVKYTREQLLELKETTQLSDEILRVQRETAAELFGEEGTWARGESV 120

Query: 121 QPN----QSQNRYSEPDNRDWRGRSAQFSATGEDRSRETIRDRDFGGHFDSRQQEGNQSS 180
             N    QS +R+SEPD+RDWR RS Q   +GE+RS + +R+       DSR  E +Q +
Sbjct: 121 VSNLVPVQSASRFSEPDSRDWRSRSTQPPPSGEERSWDNLREAK-----DSRYVEASQYN 180

Query: 181 RQDS-NSSWTRT--TSNQGGAPA-ALIKAEVPWSTRRGSLSEKDRVLKTVKGILNKLTPE 240
           RQD  NS ++R   +SNQGG PA  L+KAEVPWS RRG+LSE DRVLKTVKGILNKLTPE
Sbjct: 181 RQDQPNSQFSRANISSNQGGGPAPVLVKAEVPWSARRGNLSENDRVLKTVKGILNKLTPE 240

Query: 241 KFDLLKGQLIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNLKLPQFPSDEP 300
           K+DLLKGQLI+SGITSADILK VI+LIFDKAVLEPTFCPMYA+LCSD+N +LP FP  EP
Sbjct: 241 KYDLLKGQLIESGITSADILKGVITLIFDKAVLEPTFCPMYAKLCSDINDQLPTFPPAEP 300

Query: 301 GGREITFKRVLLNICQEAFEGADKLREEVRQMTAPEQEMERRDKERLVKLRTLGNIRLIG 360
           G +EITFKRVLLNICQEAFEGA +LREE+RQM+AP+QE ER DKE+L+KL+TLGNIRLIG
Sbjct: 301 GDKEITFKRVLLNICQEAFEGASQLREELRQMSAPDQEAERNDKEKLLKLKTLGNIRLIG 360

Query: 361 ELLKQKMVPEKIVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENAKSRRINDM 420
           ELLKQKMVPEKIVHHIVQELLG D K CPAEENVEAIC FF TIGKQLD N KS+RIND+
Sbjct: 361 ELLKQKMVPEKIVHHIVQELLGADEKVCPAEENVEAICHFFKTIGKQLDGNVKSKRINDV 420

Query: 421 YFGRLKELTTHPQLAPRLKFMVRNVLDLRANNWVPRREEIKAKTITEIHSEAERNLGLRP 480
           YF RL+ L+ +PQL  RL+FMV+N++D+R+N WVPRREE+KA+TITEIH+EAE+NLGLRP
Sbjct: 421 YFKRLQALSKNPQLELRLRFMVQNIIDMRSNGWVPRREEMKARTITEIHTEAEKNLGLRP 480

Query: 481 GATVNIRNSRGVVSGTAGNTSPGG-FPINRPGFGGMMPGMPGTRKMPGMPGIDNDNWEVP 540
           GAT N+R  RG+VS + G  SPG  +P  RPG GG+MPGMPGTR+MPGMPG+DNDNWEVP
Sbjct: 481 GATANMR--RGMVS-SGGPVSPGPVYPGGRPGAGGLMPGMPGTRRMPGMPGVDNDNWEVP 540

Query: 541 RSRPIPRGGDGSGTQPAGRGGQTPLVGKAPMLNARLLPQGTGGSISNKTSALLQGAGAST 600
           R+R + R  DG G         +P V K+  +N RLLPQG+ G +S KTSALLQG+G S 
Sbjct: 541 RTRSMSR-RDGPGPL------HSPAVSKSASMNTRLLPQGSSGIMSGKTSALLQGSG-SV 600

Query: 601 TRPVNFGPGLEPSSQVQKPVTAAAVLPLAEKPQVPAASSKQDDLEKKTLSLLEEYFSVRL 660
           +RPV      E  +Q   P+T    +   EKPQ       ++ L++KT SLLEEYF+VRL
Sbjct: 601 SRPVTV--SAERPAQSVAPLTVPVPV---EKPQPSGPKLSEEVLQRKTKSLLEEYFNVRL 660

Query: 661 LDEALQCVEELVSPAYHPEIVKQAISLALEKIPPCVEAVVKLLDYLLSKKVFTAGDIGTG 720
           L EALQCVEEL  P+YHPE VK+AISL+LEK PP VE +  LL+YLLSKKV    D+ TG
Sbjct: 661 LGEALQCVEELGLPSYHPEFVKEAISLSLEKSPPVVEPIATLLEYLLSKKVVAPKDLETG 720

Query: 721 CLQYASEIDDAGIDLPKAPMNFGEIIGKLVISGGLDFQVVKEALKKVEDDRFRKDIFDAV 780
            L Y + +DD GIDLPKAP NFGEI+GKL+++GG+DF++V+E + K+EDDRF+K + DA 
Sbjct: 721 FLLYGAMLDDIGIDLPKAPNNFGEIVGKLILAGGVDFKLVREIIGKMEDDRFQKMVVDAA 780

Query: 781 LKAVSSNASGQGLSDSLESDIEACRSL 796
           ++ V S+  G+ L  S  +DIEACR+L
Sbjct: 781 VRIVESSEQGKSLLASQAADIEACRNL 780

BLAST of MS002322 vs. TAIR 10
Match: AT2G24050.1 (MIF4G domain-containing protein / MA3 domain-containing protein )

HSP 1 Score: 790.8 bits (2041), Expect = 1.0e-228
Identity = 459/808 (56.81%), Postives = 570/808 (70.54%), Query Frame = 0

Query: 2   QQGDQTVLSLRP--GGGRTRLLGPRFDLSSSSSSSSLAFGSFSSDLPALRPHGAPPASFS 61
           QQG+ +VLSLRP  GGG++RL  PRF   SSSSS  L  G            G+    F 
Sbjct: 3   QQGEPSVLSLRPGGGGGKSRLFVPRF---SSSSSFDLTNG------------GSEETPFP 62

Query: 62  LKAGDSRFEGRERVRFTRDQLLQLREIVEVPDDILKLKREIEVEVFGEEQSWSR--GESN 121
           +K    R    ERVRF+R+++LQ RE V+V D+IL+  +EI VE+FGEEQSW     ES 
Sbjct: 63  VK----RENSGERVRFSREEILQHRESVQVSDEILRRCKEIAVELFGEEQSWGNHAAESK 122

Query: 122 QPNQSQNRYSEPDNRDWRGRSAQFSATGEDRSRETIRDRDFGGHFDSRQQEGNQSSRQDS 181
             N +Q+R++E DNRDW  RS Q   +G    +E +RD                   +++
Sbjct: 123 ITNHTQHRHTETDNRDWHSRS-QIPTSG----KEWLRD-----------------DPREA 182

Query: 182 NSSWTRTTSNQGGAPA-ALIKAEVPWSTRRGSLSEKDRVLKTVKGILNKLTPEKFDLLKG 241
            S+W      QG  P   LIKAEVPWS +RG+LS+KDRV+K+VKGILNKLTPEK++LLKG
Sbjct: 183 KSTW------QGSGPTPVLIKAEVPWSAKRGALSDKDRVVKSVKGILNKLTPEKYELLKG 242

Query: 242 QLIDSGITSADILKDVISLIFDKAVLEPTFCPMYAQLCSDLNLKLPQFPSDEPGGREITF 301
           QLID+GITSADILK+VI LIF+ A+L+PTFC MYA LC D+N +LP FPS+EPGG+EITF
Sbjct: 243 QLIDAGITSADILKEVIQLIFENAILQPTFCEMYALLCFDINGQLPSFPSEEPGGKEITF 302

Query: 302 KRVLLNICQEAFEGADKLREEVRQMTAPEQEMERRDKERLVKLRTLGNIRLIGELLKQKM 361
           KRVLLN CQEAFEGA KL+EE+RQMT P+QEMER DKE++ KLRTLGNIRLIGELLKQKM
Sbjct: 303 KRVLLNNCQEAFEGAGKLKEEIRQMTNPDQEMERMDKEKMAKLRTLGNIRLIGELLKQKM 362

Query: 362 VPEKIVHHIVQELLGPDNKTCPAEENVEAICQFFNTIGKQLDENAKSRRINDMYFGRLKE 421
           VPEKIVHHIVQELLG D K CPAE +VEA+CQFF TIGKQLD++ +SR IND YFGRLKE
Sbjct: 363 VPEKIVHHIVQELLGDDTKACPAEGDVEALCQFFITIGKQLDDSPRSRGINDTYFGRLKE 422

Query: 422 LTTHPQLAPRLKFMVRNVLDLRANNWVPRREEIKAKTITEIHSEAERNLGLRPGATVNIR 481
           L  HPQL  RL+FMV+NV+DLRAN WVPRREE+KAK I EIHSEAERNLG+RPGA  ++R
Sbjct: 423 LARHPQLELRLRFMVQNVVDLRANKWVPRREEVKAKKINEIHSEAERNLGMRPGAMASMR 482

Query: 482 ---NSRGVVSGTAGNTSPGGFPINRPGFGGMMPGMPGTRKMPGMPGIDNDNWEVPRSRPI 541
              N+R  VSG A     G   + RPG GGMMPGMPGTR MP    +D D WE+ R+R +
Sbjct: 483 NNNNNRAAVSGAADGMGLGNI-LGRPGTGGMMPGMPGTRVMP----MDEDGWEMARTRSM 542

Query: 542 PRGGDGSGTQPAGRGGQTPLVGKAPMLNARLLPQGTGGSIS--NKTSALLQGAGAST--- 601
           PRG   +  QP  R    P + K+  +N+RLLPQG+GG ++   + S LLQG G+S+   
Sbjct: 543 PRGNRQTVQQP--RFQPPPAINKSLSVNSRLLPQGSGGLLNGGGRPSPLLQGNGSSSAPQ 602

Query: 602 -TRPVNFGPGLEPSSQVQKPVTAAAVLPLAEKPQVPAASSKQDDLEKKTLSLLEEYFSVR 661
            ++P+      +P SQ Q    AA   PLA        S    +LE+KT SLLEEYFS+R
Sbjct: 603 ASKPIPTVEKPQPRSQPQPQPQAA---PLAN-------SLNAGELERKTKSLLEEYFSIR 662

Query: 662 LLDEALQCVEELVSPAYHPEIVKQAISLALEKIPPCVEAVVKLLDYLLSKKVFTAGDIGT 721
           L+DEALQCVEEL SP+YHPE+VK+ ISL LEK PP VE + KLL +L+SK V T+ D+G 
Sbjct: 663 LVDEALQCVEELKSPSYHPELVKETISLGLEKNPPLVEPIAKLLKHLISKNVLTSKDLGA 722

Query: 722 GCLQYASEIDDAGIDLPKAPMNFGEIIGKLVISGGLDFQVVKEALKKVEDDRFRKDIFDA 781
           GCL Y S +DD GIDLPKAP +FGE +G+LV +  LDF++V++ LKK+ED+ FRK + +A
Sbjct: 723 GCLLYGSMLDDIGIDLPKAPNSFGEFLGELVSAKVLDFELVRDVLKKMEDEWFRKTVLNA 746

Query: 782 VLKAVSSNASGQGLSDSLESDIEACRSL 796
           V+K+V    SGQ + DS   ++EAC+SL
Sbjct: 783 VIKSVRECPSGQSVLDSQAVEVEACQSL 746

BLAST of MS002322 vs. TAIR 10
Match: AT3G60240.2 (eukaryotic translation initiation factor 4G )

HSP 1 Score: 223.4 bits (568), Expect = 6.5e-58
Identity = 192/588 (32.65%), Postives = 284/588 (48.30%), Query Frame = 0

Query: 207  GSLSEKDRV-LKTVKGILNKLTPEKFDLLKGQLIDSGITSADILKDVISLIFDKAVLEPT 266
            G+++++++   + +K ILNKLTP+ F+ L  Q+    I +A  L  VIS IFDKA++EPT
Sbjct: 1082 GTIADEEQAKQRQLKSILNKLTPQNFEKLFEQVKSVNIDNAVTLSGVISQIFDKALMEPT 1141

Query: 267  FCPMYAQLCSDLNLKLPQFPSDEPGGREITFKRVLLNICQEAFEGADKLREEVRQMTAPE 326
            FC MYA  C  L+  LP F      G +ITFKR+LLN CQE FE  +K  EE  ++    
Sbjct: 1142 FCEMYADFCFHLSGALPDF---NENGEKITFKRLLLNKCQEEFERGEKEEEEASRVAEEG 1201

Query: 327  Q----EMERRDKERLVKLRTLGNIRLIGELLKQKMVPEKIVHHIVQELLGPDNKTCPAEE 386
            Q    E ER +K   V+ R LGNIRLIGEL K++M+ EKI+H  +Q+LLG +    P EE
Sbjct: 1202 QVEQTEEEREEKRLQVRRRMLGNIRLIGELYKKRMLTEKIMHACIQKLLGYNQD--PHEE 1261

Query: 387  NVEAICQFFNTIGKQLDENAKSRRINDMYFGRLKELTTHPQLAPRLKFMVRNVLDLRANN 446
            N+EA+C+  +TIG  +D N K++   D YF ++K L+   +L+ R++FM+ N +DLR N 
Sbjct: 1262 NIEALCKLMSTIGVMIDHN-KAKFQMDGYFEKMKMLSCKQELSSRVRFMLINAIDLRKNK 1321

Query: 447  WVPRREEIKAKTITEIHSEAERNLGLRPGATVNIRNSRGVVSGTAGNTSPGGFPINRPGF 506
            W  R +    K I E+H +A +        T   R SRG    ++G      F   R G 
Sbjct: 1322 WQERMKVEGPKKIEEVHRDAAQE-----RQTQANRLSRGPSMNSSGRRGHMEFSSPRGGG 1381

Query: 507  GGMMPGMPGTRKMPGMP---GIDNDNWEV-------PRSRPI-------------PRGGD 566
            G + P         G P   G  N +          PR  P+             P+GG 
Sbjct: 1382 GMLSPPAAQMGSYHGPPQGRGFSNQDIRFDDRPSYEPRMVPMPQRSVCEEPITLGPQGGL 1441

Query: 567  GSG-----------------TQPAGRGGQTPLVG---------KAPMLNARLLPQGTGGS 626
            G G                 TQ  G   + P  G          +P+ + R  PQ  G  
Sbjct: 1442 GQGMSIRRPAVASNTYQSDATQAGGGDSRRPAGGLNGFGSHRPASPVTHGRSSPQERG-- 1501

Query: 627  ISNKTSALLQGAGASTTRPVNFGPGLEPSSQVQKPVTAAAVLPLAEKPQVPAASSKQDDL 686
                 +A +    AS +R  +  P +  + QV +  +A         P+  A S +Q  L
Sbjct: 1502 -----TAYVHREFASLSRASDLSPEVSSARQVLQGPSAT-----VNSPRENALSEEQ--L 1561

Query: 687  EKKTLSLLEEYFSVRLLDEALQCVEELVSPAYHPEIVKQAISLALEKIPPCVEAVVKLLD 739
            E  +LS ++EY+S R  +E   C++++ SPAYHP ++   ++ + E+     + + KLL 
Sbjct: 1562 ENLSLSAIKEYYSARDENEIGMCMKDMNSPAYHPTMISLWVTDSFERKDKERDLLAKLLV 1621

BLAST of MS002322 vs. TAIR 10
Match: AT3G60240.3 (eukaryotic translation initiation factor 4G )

HSP 1 Score: 223.4 bits (568), Expect = 6.5e-58
Identity = 192/588 (32.65%), Postives = 284/588 (48.30%), Query Frame = 0

Query: 207  GSLSEKDRV-LKTVKGILNKLTPEKFDLLKGQLIDSGITSADILKDVISLIFDKAVLEPT 266
            G+++++++   + +K ILNKLTP+ F+ L  Q+    I +A  L  VIS IFDKA++EPT
Sbjct: 1084 GTIADEEQAKQRQLKSILNKLTPQNFEKLFEQVKSVNIDNAVTLSGVISQIFDKALMEPT 1143

Query: 267  FCPMYAQLCSDLNLKLPQFPSDEPGGREITFKRVLLNICQEAFEGADKLREEVRQMTAPE 326
            FC MYA  C  L+  LP F      G +ITFKR+LLN CQE FE  +K  EE  ++    
Sbjct: 1144 FCEMYADFCFHLSGALPDF---NENGEKITFKRLLLNKCQEEFERGEKEEEEASRVAEEG 1203

Query: 327  Q----EMERRDKERLVKLRTLGNIRLIGELLKQKMVPEKIVHHIVQELLGPDNKTCPAEE 386
            Q    E ER +K   V+ R LGNIRLIGEL K++M+ EKI+H  +Q+LLG +    P EE
Sbjct: 1204 QVEQTEEEREEKRLQVRRRMLGNIRLIGELYKKRMLTEKIMHACIQKLLGYNQD--PHEE 1263

Query: 387  NVEAICQFFNTIGKQLDENAKSRRINDMYFGRLKELTTHPQLAPRLKFMVRNVLDLRANN 446
            N+EA+C+  +TIG  +D N K++   D YF ++K L+   +L+ R++FM+ N +DLR N 
Sbjct: 1264 NIEALCKLMSTIGVMIDHN-KAKFQMDGYFEKMKMLSCKQELSSRVRFMLINAIDLRKNK 1323

Query: 447  WVPRREEIKAKTITEIHSEAERNLGLRPGATVNIRNSRGVVSGTAGNTSPGGFPINRPGF 506
            W  R +    K I E+H +A +        T   R SRG    ++G      F   R G 
Sbjct: 1324 WQERMKVEGPKKIEEVHRDAAQE-----RQTQANRLSRGPSMNSSGRRGHMEFSSPRGGG 1383

Query: 507  GGMMPGMPGTRKMPGMP---GIDNDNWEV-------PRSRPI-------------PRGGD 566
            G + P         G P   G  N +          PR  P+             P+GG 
Sbjct: 1384 GMLSPPAAQMGSYHGPPQGRGFSNQDIRFDDRPSYEPRMVPMPQRSVCEEPITLGPQGGL 1443

Query: 567  GSG-----------------TQPAGRGGQTPLVG---------KAPMLNARLLPQGTGGS 626
            G G                 TQ  G   + P  G          +P+ + R  PQ  G  
Sbjct: 1444 GQGMSIRRPAVASNTYQSDATQAGGGDSRRPAGGLNGFGSHRPASPVTHGRSSPQERG-- 1503

Query: 627  ISNKTSALLQGAGASTTRPVNFGPGLEPSSQVQKPVTAAAVLPLAEKPQVPAASSKQDDL 686
                 +A +    AS +R  +  P +  + QV +  +A         P+  A S +Q  L
Sbjct: 1504 -----TAYVHREFASLSRASDLSPEVSSARQVLQGPSAT-----VNSPRENALSEEQ--L 1563

Query: 687  EKKTLSLLEEYFSVRLLDEALQCVEELVSPAYHPEIVKQAISLALEKIPPCVEAVVKLLD 739
            E  +LS ++EY+S R  +E   C++++ SPAYHP ++   ++ + E+     + + KLL 
Sbjct: 1564 ENLSLSAIKEYYSARDENEIGMCMKDMNSPAYHPTMISLWVTDSFERKDKERDLLAKLLV 1623