MS002312 (gene) Bitter gourd (TR) v1

Overview


Name	MS002312
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	DNA-directed RNA polymerase subunit beta
Location	scaffold30: 4046481 .. 4046940 (-)
RNA-Seq Expression	MS002312
Synteny	MS002312

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATAGCGATGGCAGATTCCACCTTTATCTTCTCCGGTCCTTCCGCCGTATCTCGCTCCCTTTTCAACATCAACAATCGCAGAAATTTTCGAACCGCATTGGCAAAACGAACCGACGATTCCACCGCCACTAAATCGAATCAAGAAAATCAATTCGAGTTTCCGTCGCTGAGAGTTCCAAATCCACTTGTCGCTCGATTTGTCGTTTCGGTTCTCGGATTAGGGTTCGTCGATGCAGGGTACAAAAATTAAGTTAATTTGTCTTCTTCGTCGATCTTTTTTGTTTTTTTGAATTGAACTTGAAGGACGGATTGGAATTAATTTGATCAGGTACAGTGGAGACTGGTCTAGAATTGGAGCAATAACTAAAGAGAGCGAAGATTTGCTGAAGATTGGAGCTTTCTTAGTGGTTCCGTTTTGCGTATTTCTGATATCTTCTTTTGCCAAACAGACGGATTCTTCG

mRNA sequence

ATAGCGATGGCAGATTCCACCTTTATCTTCTCCGGTCCTTCCGCCGTATCTCGCTCCCTTTTCAACATCAACAATCGCAGAAATTTTCGAACCGCATTGGCAAAACGAACCGACGATTCCACCGCCACTAAATCGAATCAAGAAAATCAATTCGAGTTTCCGTCGCTGAGAGTTCCAAATCCACTTGTCGCTCGATTTGTCGTTTCGGTTCTCGGATTAGGGTTCGTCGATGCAGGGTACAGTGGAGACTGGTCTAGAATTGGAGCAATAACTAAAGAGAGCGAAGATTTGCTGAAGATTGGAGCTTTCTTAGTGGTTCCGTTTTGCGTATTTCTGATATCTTCTTTTGCCAAACAGACGGATTCTTCG

Coding sequence (CDS)

ATAGCGATGGCAGATTCCACCTTTATCTTCTCCGGTCCTTCCGCCGTATCTCGCTCCCTTTTCAACATCAACAATCGCAGAAATTTTCGAACCGCATTGGCAAAACGAACCGACGATTCCACCGCCACTAAATCGAATCAAGAAAATCAATTCGAGTTTCCGTCGCTGAGAGTTCCAAATCCACTTGTCGCTCGATTTGTCGTTTCGGTTCTCGGATTAGGGTTCGTCGATGCAGGGTACAGTGGAGACTGGTCTAGAATTGGAGCAATAACTAAAGAGAGCGAAGATTTGCTGAAGATTGGAGCTTTCTTAGTGGTTCCGTTTTGCGTATTTCTGATATCTTCTTTTGCCAAACAGACGGATTCTTCG

Protein sequence

IAMADSTFIFSGPSAVSRSLFNINNRRNFRTALAKRTDDSTATKSNQENQFEFPSLRVPNPLVARFVVSVLGLGFVDAGYSGDWSRIGAITKESEDLLKIGAFLVVPFCVFLISSFAKQTDSS

Homology

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BLAST of MS002312 vs. NCBI nr
Match: XP_022135305.1 (uncharacterized protein LOC111007301 [Momordica charantia])

HSP 1 Score: 238.0 bits (606), Expect = 4.2e-59
Identity = 120/121 (99.17%), Postives = 121/121 (100.00%), Query Frame = 0


Query: 3   MADSTFIFSGPSAVSRSLFNINNRRNFRTALAKRTDDSTATKSNQENQFEFPSLRVPNPL 62
           MADSTFIFSGPSAVSRSLFN+NNRRNFRTALAKRTDDSTATKSNQENQFEFPSLRVPNPL
Sbjct: 1   MADSTFIFSGPSAVSRSLFNVNNRRNFRTALAKRTDDSTATKSNQENQFEFPSLRVPNPL 60

Query: 63  VARFVVSVLGLGFVDAGYSGDWSRIGAITKESEDLLKIGAFLVVPFCVFLISSFAKQTDS 122
           VARFVVSVLGLGFVDAGYSGDWSRIGAITKESEDLLKIGAFLVVPFCVFLISSFAKQTDS
Sbjct: 61  VARFVVSVLGLGFVDAGYSGDWSRIGAITKESEDLLKIGAFLVVPFCVFLISSFAKQTDS 120

Query: 123 S 124
           S
Sbjct: 121 S 121

[Close]

BLAST of MS002312 vs. NCBI nr
Match: XP_008443827.1 (PREDICTED: uncharacterized protein LOC103487329 [Cucumis melo])

HSP 1 Score: 164.9 bits (416), Expect = 4.5e-37
Identity = 90/127 (70.87%), Postives = 100/127 (78.74%), Query Frame = 0


Query: 1   IAMADSTFIFSGPSAVSRSLFN---INNRRNFRTALAKRTDDSTATKSNQENQFEFPSLR 60
           +AM DS+FIF G SAV   L N    NN R F    AKRT DS +T S Q NQF+FPSLR
Sbjct: 1   MAMVDSSFIFIGISAVPHPLLNNNKNNNGRKFGITSAKRTQDSDSTSSTQPNQFKFPSLR 60

Query: 61  VPNPLVARFVVSVLGLGFVDAGYSGDWSRIGAITKESEDLLKIGAFLVVPFCVFLISSFA 120
           V NPL+AR VVSVLGLGFVDAGYSGDWSRIGAITKE+E+LLKIGAFLVVPFCVFL+ SF+
Sbjct: 61  VSNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEELLKIGAFLVVPFCVFLVFSFS 120

Query: 121 K-QTDSS 124
           K  +DSS
Sbjct: 121 KFDSDSS 127

[Close]

BLAST of MS002312 vs. NCBI nr
Match: XP_004146667.1 (uncharacterized protein LOC101206545 [Cucumis sativus] >KGN65187.1 hypothetical protein Csa_020133 [Cucumis sativus])

HSP 1 Score: 160.2 bits (404), Expect = 1.1e-35
Identity = 88/126 (69.84%), Postives = 97/126 (76.98%), Query Frame = 0


Query: 1   IAMADSTFIFSGPSAVSRSLFNINNRRNFRTALAKRT--DDSTATKSNQENQFEFPSLRV 60
           +AM DS+FIF G S V   L N NN R F    AKRT   DS +  S Q NQF+FPSLRV
Sbjct: 1   MAMVDSSFIFIGFSPVPHPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLRV 60

Query: 61  PNPLVARFVVSVLGLGFVDAGYSGDWSRIGAITKESEDLLKIGAFLVVPFCVFLISSFAK 120
            NPL+AR VVSVLGLGFVDAGYSGDWSRIGAITKE+EDLLKIGA LVVPFCVFL+ SF+K
Sbjct: 61  SNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSK 120

Query: 121 -QTDSS 124
             +DSS
Sbjct: 121 YDSDSS 126

[Close]

BLAST of MS002312 vs. NCBI nr
Match: XP_023531156.1 (uncharacterized protein LOC111793484 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 159.1 bits (401), Expect = 2.5e-35
Identity = 87/122 (71.31%), Postives = 98/122 (80.33%), Query Frame = 0


Query: 3   MADSTFIFSGPSAVSRS-LFNINNRRNFRTALAKRTDDSTATKSNQENQFEFPSLRVPNP 62
           +ADSTFIF+G SA+    L + NN R F T  AKRT DS  + S    Q +FPSLRV NP
Sbjct: 3   IADSTFIFTGLSALPHPLLLSNNNARKFGTTSAKRTGDSD-SDSISSTQLKFPSLRVANP 62

Query: 63  LVARFVVSVLGLGFVDAGYSGDWSRIGAITKESEDLLKIGAFLVVPFCVFLISSFAKQTD 122
           L+AR VV+VLGLGFVDAGYSGDWSRIGAITKE+EDLLKIGAFLVVPFCVFLI SF++QTD
Sbjct: 63  LLARSVVAVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGAFLVVPFCVFLIFSFSEQTD 122

Query: 123 SS 124
           SS
Sbjct: 123 SS 123

[Close]

BLAST of MS002312 vs. NCBI nr
Match: XP_022933449.1 (uncharacterized protein LOC111440861 [Cucurbita moschata] >KAG6587801.1 hypothetical protein SDJN03_16366, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035696.1 hypothetical protein SDJN02_02494, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 157.9 bits (398), Expect = 5.5e-35
Identity = 86/122 (70.49%), Postives = 98/122 (80.33%), Query Frame = 0


Query: 3   MADSTFIFSGPSAVSRS-LFNINNRRNFRTALAKRTDDSTATKSNQENQFEFPSLRVPNP 62
           +ADS+FIF+G SA+    L + NN R F T  AKRT DS +  S    Q +FPSLRV NP
Sbjct: 3   IADSSFIFTGLSALPHPLLLSNNNARKFGTTSAKRTGDSDSISS---TQLKFPSLRVANP 62

Query: 63  LVARFVVSVLGLGFVDAGYSGDWSRIGAITKESEDLLKIGAFLVVPFCVFLISSFAKQTD 122
           L+AR VV+VLGLGFVDAGYSGDWSRIGAITKE+EDLLKIGAFLVVPFCVFLI SF++QTD
Sbjct: 63  LLARSVVAVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGAFLVVPFCVFLIFSFSEQTD 121

Query: 123 SS 124
           SS
Sbjct: 123 SS 121

[Close]

BLAST of MS002312 vs. ExPASy TrEMBL
Match: A0A6J1C0R6 (uncharacterized protein LOC111007301 OS=Momordica charantia OX=3673 GN=LOC111007301 PE=4 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 2.0e-59
Identity = 120/121 (99.17%), Postives = 121/121 (100.00%), Query Frame = 0


Query: 3   MADSTFIFSGPSAVSRSLFNINNRRNFRTALAKRTDDSTATKSNQENQFEFPSLRVPNPL 62
           MADSTFIFSGPSAVSRSLFN+NNRRNFRTALAKRTDDSTATKSNQENQFEFPSLRVPNPL
Sbjct: 1   MADSTFIFSGPSAVSRSLFNVNNRRNFRTALAKRTDDSTATKSNQENQFEFPSLRVPNPL 60

Query: 63  VARFVVSVLGLGFVDAGYSGDWSRIGAITKESEDLLKIGAFLVVPFCVFLISSFAKQTDS 122
           VARFVVSVLGLGFVDAGYSGDWSRIGAITKESEDLLKIGAFLVVPFCVFLISSFAKQTDS
Sbjct: 61  VARFVVSVLGLGFVDAGYSGDWSRIGAITKESEDLLKIGAFLVVPFCVFLISSFAKQTDS 120

Query: 123 S 124
           S
Sbjct: 121 S 121

[Close]

BLAST of MS002312 vs. ExPASy TrEMBL
Match: A0A1S3B903 (uncharacterized protein LOC103487329 OS=Cucumis melo OX=3656 GN=LOC103487329 PE=4 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 2.2e-37
Identity = 90/127 (70.87%), Postives = 100/127 (78.74%), Query Frame = 0


Query: 1   IAMADSTFIFSGPSAVSRSLFN---INNRRNFRTALAKRTDDSTATKSNQENQFEFPSLR 60
           +AM DS+FIF G SAV   L N    NN R F    AKRT DS +T S Q NQF+FPSLR
Sbjct: 1   MAMVDSSFIFIGISAVPHPLLNNNKNNNGRKFGITSAKRTQDSDSTSSTQPNQFKFPSLR 60

Query: 61  VPNPLVARFVVSVLGLGFVDAGYSGDWSRIGAITKESEDLLKIGAFLVVPFCVFLISSFA 120
           V NPL+AR VVSVLGLGFVDAGYSGDWSRIGAITKE+E+LLKIGAFLVVPFCVFL+ SF+
Sbjct: 61  VSNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEELLKIGAFLVVPFCVFLVFSFS 120

Query: 121 K-QTDSS 124
           K  +DSS
Sbjct: 121 KFDSDSS 127

[Close]

BLAST of MS002312 vs. ExPASy TrEMBL
Match: A0A0A0LWH4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G257930 PE=4 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 5.4e-36
Identity = 88/126 (69.84%), Postives = 97/126 (76.98%), Query Frame = 0


Query: 1   IAMADSTFIFSGPSAVSRSLFNINNRRNFRTALAKRT--DDSTATKSNQENQFEFPSLRV 60
           +AM DS+FIF G S V   L N NN R F    AKRT   DS +  S Q NQF+FPSLRV
Sbjct: 1   MAMVDSSFIFIGFSPVPHPLLNNNNGRKFGITSAKRTQDSDSDSASSTQPNQFKFPSLRV 60

Query: 61  PNPLVARFVVSVLGLGFVDAGYSGDWSRIGAITKESEDLLKIGAFLVVPFCVFLISSFAK 120
            NPL+AR VVSVLGLGFVDAGYSGDWSRIGAITKE+EDLLKIGA LVVPFCVFL+ SF+K
Sbjct: 61  SNPLLARSVVSVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGALLVVPFCVFLVFSFSK 120

Query: 121 -QTDSS 124
             +DSS
Sbjct: 121 YDSDSS 126

[Close]

BLAST of MS002312 vs. ExPASy TrEMBL
Match: A0A6J1EZT4 (uncharacterized protein LOC111440861 OS=Cucurbita moschata OX=3662 GN=LOC111440861 PE=4 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 2.7e-35
Identity = 86/122 (70.49%), Postives = 98/122 (80.33%), Query Frame = 0


Query: 3   MADSTFIFSGPSAVSRS-LFNINNRRNFRTALAKRTDDSTATKSNQENQFEFPSLRVPNP 62
           +ADS+FIF+G SA+    L + NN R F T  AKRT DS +  S    Q +FPSLRV NP
Sbjct: 3   IADSSFIFTGLSALPHPLLLSNNNARKFGTTSAKRTGDSDSISS---TQLKFPSLRVANP 62

Query: 63  LVARFVVSVLGLGFVDAGYSGDWSRIGAITKESEDLLKIGAFLVVPFCVFLISSFAKQTD 122
           L+AR VV+VLGLGFVDAGYSGDWSRIGAITKE+EDLLKIGAFLVVPFCVFLI SF++QTD
Sbjct: 63  LLARSVVAVLGLGFVDAGYSGDWSRIGAITKETEDLLKIGAFLVVPFCVFLIFSFSEQTD 121

Query: 123 SS 124
           SS
Sbjct: 123 SS 121

[Close]

BLAST of MS002312 vs. ExPASy TrEMBL
Match: A0A6J1KT82 (uncharacterized protein LOC111498016 OS=Cucurbita maxima OX=3661 GN=LOC111498016 PE=4 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 7.8e-35
Identity = 85/122 (69.67%), Postives = 97/122 (79.51%), Query Frame = 0


Query: 3   MADSTFIFSGPSAVSRSL-FNINNRRNFRTALAKRTDDSTATKSNQENQFEFPSLRVPNP 62
           +ADS+FIF+G SA+   L  + NN R F T  AKRT DS +  S    Q +FPSLRV NP
Sbjct: 3   IADSSFIFTGLSALPHPLILSNNNARKFGTTSAKRTGDSDSISS---TQLKFPSLRVSNP 62

Query: 63  LVARFVVSVLGLGFVDAGYSGDWSRIGAITKESEDLLKIGAFLVVPFCVFLISSFAKQTD 122
           L+AR VV+VLGLGFVDAGYSGDWSRIGAITKE+ED LKIGAFLVVPFCVFLI SF++QTD
Sbjct: 63  LLARSVVAVLGLGFVDAGYSGDWSRIGAITKETEDFLKIGAFLVVPFCVFLIFSFSEQTD 121

Query: 123 SS 124
           SS
Sbjct: 123 SS 121

[Close]

BLAST of MS002312 vs. TAIR 10
Match: AT4G30845.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; Has 16 Blast hits to 16 proteins in 8 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 16; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 100.5 bits (249), Expect = 9.8e-22
Identity = 55/102 (53.92%), Postives = 71/102 (69.61%), Query Frame = 0


Query: 22  NINNRRNFR---TALAKRTDDSTATKSNQENQFEFPSLRVPNPLVARFVVSVLGLGFVDA 81
           N N  R+F    T ++KR D S   KSN+E       ++VPN +VAR  ++VL LGF+DA
Sbjct: 15  NDNKLRSFANGVTIMSKRRDFS--EKSNEER--PILRIKVPNTIVARSAIAVLSLGFIDA 74

Query: 82  GYSGDWSRIGAITKESEDLLKIGAFLVVPFCVFLISSFAKQT 121
           GYSGDWSRIG I+KE+E+LLKI AFLVVP C+FL  SF+  +
Sbjct: 75  GYSGDWSRIGVISKETEELLKIAAFLVVPLCIFLALSFSNDS 112

The following BLAST results are available for this feature:

BLAST of MS002312 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135305.1	4.2e-59	99.17	uncharacterized protein LOC111007301 [Momordica charantia]	[more]
XP_008443827.1	4.5e-37	70.87	PREDICTED: uncharacterized protein LOC103487329 [Cucumis melo]	[more]
XP_004146667.1	1.1e-35	69.84	uncharacterized protein LOC101206545 [Cucumis sativus] >KGN65187.1 hypothetical ...	[more]
XP_023531156.1	2.5e-35	71.31	uncharacterized protein LOC111793484 [Cucurbita pepo subsp. pepo]	[more]
XP_022933449.1	5.5e-35	70.49	uncharacterized protein LOC111440861 [Cucurbita moschata] >KAG6587801.1 hypothet...	[more]

BLAST of MS002312 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS002312 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C0R6	2.0e-59	99.17	uncharacterized protein LOC111007301 OS=Momordica charantia OX=3673 GN=LOC111007...	[more]
A0A1S3B903	2.2e-37	70.87	uncharacterized protein LOC103487329 OS=Cucumis melo OX=3656 GN=LOC103487329 PE=...	[more]
A0A0A0LWH4	5.4e-36	69.84	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G257930 PE=4 SV=1	[more]
A0A6J1EZT4	2.7e-35	70.49	uncharacterized protein LOC111440861 OS=Cucurbita moschata OX=3662 GN=LOC1114408...	[more]
A0A6J1KT82	7.8e-35	69.67	uncharacterized protein LOC111498016 OS=Cucurbita maxima OX=3661 GN=LOC111498016...	[more]

BLAST of MS002312 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G30845.1	9.8e-22	53.92	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR38389	DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA	coord: 10..121

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS002312.1	MS002312.1	mRNA