MS002288 (gene) Bitter gourd (TR) v1

BLAST of MS002288 vs. NCBI nr
Match: XP_022134832.1 (E3 ubiquitin-protein ligase XBAT32 [Momordica charantia])

HSP 1 Score: 966.8 bits (2498), Expect = 6.7e-278
Identity = 474/477 (99.37%), Postives = 476/477 (99.79%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGHWEVVLILILFRASIHKADYLNGGT 120
           HGHHEIVYLLLQSGVD+NLRNYRGQTALMQACQHGHWEVVLIL+LFRASIHKADYLNGGT
Sbjct: 61  HGHHEIVYLLLQSGVDVNLRNYRGQTALMQACQHGHWEVVLILVLFRASIHKADYLNGGT 120

Query: 121 AIHLAASNGHSRCIRLLLADYIPSIANFRNIMSKITDNEESTSGFDHRALSQVINQPADG 180
           AIHLAASNGHSRCIRLLLADYIPSIANFRNIMSKITDNEESTSGFDHRALSQVINQPADG
Sbjct: 121 AIHLAASNGHSRCIRLLLADYIPSIANFRNIMSKITDNEESTSGFDHRALSQVINQPADG 180

Query: 181 GITALHMAALNGQVGSVQLLLDLGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           GITALHMAALNGQVGSVQLLLDLGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCC
Sbjct: 181 GITALHMAALNGQVGSVQLLLDLGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCC 240

Query: 241 QLLIARGASLTIENANGWTPLMVAHSWHKSWLEDILSSEPVGQPKLILSPYLSLPLMSIV 300
           QLLIARGASLTIENANGWTPLMVAHSWHKSWLEDILSSEPVGQPKLILSPYLSLPLMSIV
Sbjct: 241 QLLIARGASLTIENANGWTPLMVAHSWHKSWLEDILSSEPVGQPKLILSPYLSLPLMSIV 300

Query: 301 QIARDCGWSTKSALGCQDPCVVCLERKCAVAAEGCDHELCTRCALYLCSTNCGSTTARGP 360
           QIARDCGWSTKSALGCQDPCVVCLERKCAVAAEGCDHE CTRCALYLCSTNCGSTTARGP
Sbjct: 301 QIARDCGWSTKSALGCQDPCVVCLERKCAVAAEGCDHEFCTRCALYLCSTNCGSTTARGP 360

Query: 361 PGSVSCPLCRNGIVSFVKLPEKSPVVKEIVRTSLSLSSTCQCSHDSPLKSTSLTTQLRTL 420
           PGSVSCPLCRNGIVSFVKLPEKSPVVKEIVRTSLSLSSTCQCSHDSPLKSTSLTTQLRTL
Sbjct: 361 PGSVSCPLCRNGIVSFVKLPEKSPVVKEIVRTSLSLSSTCQCSHDSPLKSTSLTTQLRTL 420

Query: 421 ELCRTHDSPLGLPFRCQRFPSIKFNSNLCRETPDICHSLVPRYAHWNLRNHLARCSR 478
           ELCRTHDSPLGLPFRCQRFPSIKFNSNLCRETPDICHSLVPRYAHWNLRNHLARCSR
Sbjct: 421 ELCRTHDSPLGLPFRCQRFPSIKFNSNLCRETPDICHSLVPRYAHWNLRNHLARCSR 477

BLAST of MS002288 vs. NCBI nr
Match: XP_038879942.1 (probable E3 ubiquitin-protein ligase XBOS32 isoform X1 [Benincasa hispida])

HSP 1 Score: 791.6 bits (2043), Expect = 3.8e-225
Identity = 393/475 (82.74%), Postives = 429/475 (90.32%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGHWEVVLILILFRASIHKADYLNGGT 120
           HGHHEIVYLLLQSGVDIN+RNYRGQTALMQACQHGHWEVVLIL+LF A++HKADYLNGGT
Sbjct: 61  HGHHEIVYLLLQSGVDINIRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGGT 120

Query: 121 AIHLAASNGHSRCIRLLLADYIPSIANFRNIMSKITDNEESTSGFDHRALSQVINQPADG 180
           A+HLAA+NGHSRCIRLLLADYIPS+ NFR IMS++TDNEES   FDHRALS++INQ ADG
Sbjct: 121 ALHLAATNGHSRCIRLLLADYIPSMPNFREIMSQLTDNEESILEFDHRALSKIINQTADG 180

Query: 181 GITALHMAALNGQVGSVQLLLDLGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           GITALHMAALNGQV SVQLLL+ GASVSEVT+GDGT+IDLIGAGSTALHYAACGGNAKCC
Sbjct: 181 GITALHMAALNGQVESVQLLLEFGASVSEVTLGDGTIIDLIGAGSTALHYAACGGNAKCC 240

Query: 241 QLLIARGASLTIENANGWTPLMVAHSWHKSWLEDILSSEPVGQPKLILSPYLSLPLMSIV 300
           QLLIARGASLT  N NGWTPLMVA +W+KSWLEDIL++EPV  P+LI S Y+SLPLMSIV
Sbjct: 241 QLLIARGASLTARNENGWTPLMVARTWNKSWLEDILTAEPVDLPRLIPSRYISLPLMSIV 300

Query: 301 QIARDCGWSTKSALGCQDPCVVCLERKCAVAAEGCDHELCTRCALYLCSTNCGSTTARGP 360
           QIAR+ GW++KSA GCQDPCVVCLERKC VAAEGC HE CTRCALY CSTN G+T +RGP
Sbjct: 301 QIAREYGWTSKSAPGCQDPCVVCLERKCTVAAEGCGHEFCTRCALYFCSTNYGTTISRGP 360

Query: 361 PGSVSCPLCRNGIVSFVKLPEKSPVVKEIVRTSLSLSSTCQCSHDSPLKSTSLTTQLRTL 420
           PGSVSCPLCRNGIVSFVKLP K+P VK+I RTSLSL S CQCSH++P K T LTTQLR+L
Sbjct: 361 PGSVSCPLCRNGIVSFVKLPGKAP-VKKIARTSLSL-SMCQCSHETPSKPTPLTTQLRSL 420

Query: 421 ELCRTHDSPLGLPFRCQRFPSIKFNSNLCRETPDICHSLVPRYAHWNLRNHLARC 476
           ELCR H +PLG+   CQR PSI    NLC +TPD+CHSLVPR A+ NL+NHLARC
Sbjct: 421 ELCRVHYTPLGVSVCCQRLPSI----NLCWKTPDVCHSLVPRCANRNLQNHLARC 469

BLAST of MS002288 vs. NCBI nr
Match: TYJ95947.1 (E3 ubiquitin-protein ligase XBAT32 [Cucumis melo var. makuwa])

HSP 1 Score: 791.6 bits (2043), Expect = 3.8e-225
Identity = 396/476 (83.19%), Postives = 429/476 (90.13%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MK LSAVV+SFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKFLSAVVHSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGHWEVVLILILFRASIHKADYLNGGT 120
           HGHHEIVYLLL SGVDINLRNYRGQTALMQACQHGHWEVVLIL+LF A++HKADYLNG T
Sbjct: 61  HGHHEIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWT 120

Query: 121 AIHLAASNGHSRCIRLLLADYIPSIANFRNIMSK-ITDNEESTSGFDHRALSQVINQPAD 180
           A+HLAA+NGHSRCIRLLLADYIPSI NFR IMS+  TD+EES S FDHRALSQ+INQ AD
Sbjct: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSRQSTDSEESISEFDHRALSQIINQKAD 180

Query: 181 GGITALHMAALNGQVGSVQLLLDLGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240
           GGITALHMAALNGQV SVQLLL+ GASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC
Sbjct: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240

Query: 241 CQLLIARGASLTIENANGWTPLMVAHSWHKSWLEDILSSEPVGQPKLILSPYLSLPLMSI 300
           CQLLIARGASLT EN NGWTPLMV+ SW+KSWLEDIL++EPV +PKLI S Y+SLPLMSI
Sbjct: 241 CQLLIARGASLTTENENGWTPLMVSRSWNKSWLEDILTTEPVERPKLIPSRYISLPLMSI 300

Query: 301 VQIARDCGWSTKSALGCQDPCVVCLERKCAVAAEGCDHELCTRCALYLCSTNCGSTTARG 360
           VQIAR+ GWS+KSA GCQDPCVVCLERKC VAA GCDHE CTRCALYLCSTNCG+T +RG
Sbjct: 301 VQIAREYGWSSKSAPGCQDPCVVCLERKCTVAAGGCDHEFCTRCALYLCSTNCGTTISRG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPEKSPVVKEIVRTSLSLSSTCQCSHDSPLKSTSLTTQLRT 420
           PPGSVSCPLCRNGIVSFVKLP K+ + KEI RTSLS    CQCSH++PLK T LTTQLR+
Sbjct: 361 PPGSVSCPLCRNGIVSFVKLPGKA-LAKEIARTSLSF-PICQCSHETPLKPTPLTTQLRS 420

Query: 421 LELCRTHDSPLGLPFRCQRFPSIKFNSNLCRETPDICHSLVPRYAHWNLRNHLARC 476
           LELCR HD+PLG+ F C + PSI    +LCR+ PDICHSLVPR A+ NL+NHL+RC
Sbjct: 421 LELCRVHDTPLGIYFCCHKLPSI----SLCRKAPDICHSLVPRCANGNLQNHLSRC 470

BLAST of MS002288 vs. NCBI nr
Match: XP_008443799.1 (PREDICTED: E3 ubiquitin-protein ligase XBAT32 [Cucumis melo] >KAA0035132.1 E3 ubiquitin-protein ligase XBAT32 [Cucumis melo var. makuwa])

HSP 1 Score: 788.9 bits (2036), Expect = 2.5e-224
Identity = 395/476 (82.98%), Postives = 428/476 (89.92%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MK LSAVV+SFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKFLSAVVHSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGHWEVVLILILFRASIHKADYLNGGT 120
           HGHHEIVYLLL SGVDINLRNYRGQTALMQACQHGHWEVVLIL+LF A++HKADYLNG T
Sbjct: 61  HGHHEIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWT 120

Query: 121 AIHLAASNGHSRCIRLLLADYIPSIANFRNIMSK-ITDNEESTSGFDHRALSQVINQPAD 180
           A+HLAA+NGHSRCIRLLLADYIPSI NFR IMS+  TD+EES S FDHRALSQ+INQ AD
Sbjct: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSRQSTDSEESISEFDHRALSQIINQKAD 180

Query: 181 GGITALHMAALNGQVGSVQLLLDLGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240
           GGITALHMAALNGQV SVQLLL+ GASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC
Sbjct: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240

Query: 241 CQLLIARGASLTIENANGWTPLMVAHSWHKSWLEDILSSEPVGQPKLILSPYLSLPLMSI 300
           CQLLIARGASLT EN NGWTPLMV+ SW+KSWLEDIL++EPV +PKLI S Y+SLPLMSI
Sbjct: 241 CQLLIARGASLTTENENGWTPLMVSRSWNKSWLEDILTTEPVERPKLIPSRYISLPLMSI 300

Query: 301 VQIARDCGWSTKSALGCQDPCVVCLERKCAVAAEGCDHELCTRCALYLCSTNCGSTTARG 360
           VQIAR+ GWS+KSA GCQDPCVVCLERKC VAA GCDHE CTRCALYLCSTNCG+T +RG
Sbjct: 301 VQIAREYGWSSKSAPGCQDPCVVCLERKCTVAAGGCDHEFCTRCALYLCSTNCGTTISRG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPEKSPVVKEIVRTSLSLSSTCQCSHDSPLKSTSLTTQLRT 420
           PPGSVSCPLCRNGIVSFVKLP K+ + KEI RTSLS    CQCSH++P K T LTTQLR+
Sbjct: 361 PPGSVSCPLCRNGIVSFVKLPGKA-LAKEIARTSLSF-PICQCSHETPPKPTPLTTQLRS 420

Query: 421 LELCRTHDSPLGLPFRCQRFPSIKFNSNLCRETPDICHSLVPRYAHWNLRNHLARC 476
           LELCR HD+PLG+ F C + PSI    +LCR+ PDICHSLVPR A+ NL+NHL+RC
Sbjct: 421 LELCRVHDTPLGIYFCCHKLPSI----SLCRKAPDICHSLVPRCANGNLQNHLSRC 470

BLAST of MS002288 vs. NCBI nr
Match: XP_004149881.1 (E3 ubiquitin-protein ligase XBAT32 [Cucumis sativus] >KGN65279.1 hypothetical protein Csa_019889 [Cucumis sativus])

HSP 1 Score: 781.6 bits (2017), Expect = 4.0e-222
Identity = 392/476 (82.35%), Postives = 424/476 (89.08%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MK LSAVV+SFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKFLSAVVHSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGHWEVVLILILFRASIHKADYLNGGT 120
           HGHHEIVYLLL SGVDINLRNYRGQTALMQACQHGHWEVVLIL+LF A++HKADYLNG T
Sbjct: 61  HGHHEIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWT 120

Query: 121 AIHLAASNGHSRCIRLLLADYIPSIANFRNIMSK-ITDNEESTSGFDHRALSQVINQPAD 180
           A+HLAA+NGHSRCIRLLLADYIPSI NFR IMS+ +TDNEES S FDHRALSQ+INQ AD
Sbjct: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSRQLTDNEESISEFDHRALSQIINQKAD 180

Query: 181 GGITALHMAALNGQVGSVQLLLDLGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240
           GGITALHMAALNGQV SVQLLL+ GASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC
Sbjct: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240

Query: 241 CQLLIARGASLTIENANGWTPLMVAHSWHKSWLEDILSSEPVGQPKLILSPYLSLPLMSI 300
           CQLLIARGASLT EN NGWTPLMV+ SW+K+WLEDIL++EPV +PKLI S Y+SLPLMSI
Sbjct: 241 CQLLIARGASLTTENENGWTPLMVSRSWNKNWLEDILTTEPVERPKLIPSRYISLPLMSI 300

Query: 301 VQIARDCGWSTKSALGCQDPCVVCLERKCAVAAEGCDHELCTRCALYLCSTNCGSTTARG 360
           VQIAR+ GW++KSA GCQDPCVVCLERKC VAAEGCDHE CTRCALYLCSTNCG+T +RG
Sbjct: 301 VQIAREYGWTSKSAPGCQDPCVVCLERKCTVAAEGCDHEFCTRCALYLCSTNCGTTISRG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPEKSPVVKEIVRTSLSLSSTCQCSHDSPLKSTSLTTQLRT 420
           PPGSVSCPLCRNGIVSF KL  K+ + KEI RT LS     QCS ++P K T LTTQLR+
Sbjct: 361 PPGSVSCPLCRNGIVSFNKLRSKA-LAKEIARTRLSFPIR-QCSQETPSKPTPLTTQLRS 420

Query: 421 LELCRTHDSPLGLPFRCQRFPSIKFNSNLCRETPDICHSLVPRYAHWNLRNHLARC 476
           LELCR HD+PLG+ F C R PSI    NLCR+TPDICHSLVPR A+ N +NHL RC
Sbjct: 421 LELCRVHDTPLGIYFCCHRLPSI----NLCRKTPDICHSLVPRCANGNFQNHLGRC 470

BLAST of MS002288 vs. ExPASy Swiss-Prot
Match: Q6NLQ8 (E3 ubiquitin-protein ligase XBAT32 OS=Arabidopsis thaliana OX=3702 GN=XBAT32 PE=1 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 6.7e-156
Identity = 295/458 (64.41%), Postives = 346/458 (75.55%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           M+ LS V NSFGCSASGERLVSAARDGD QEA+ALL+YNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MRFLSLVGNSFGCSASGERLVSAARDGDLQEAKALLDYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGHWEVVLILILFRASIHKADYLNGGT 120
            GHHEIV LL++SGVDINLRNYRGQTALMQACQHGHWEVVLILILF A+IH++DYLNGGT
Sbjct: 61  QGHHEIVSLLVESGVDINLRNYRGQTALMQACQHGHWEVVLILILFGANIHRSDYLNGGT 120

Query: 121 AIHLAASNGHSRCIRLLLADYIPSIANFRNIMSKITDNEESTSGFDHRALSQVINQPADG 180
           A+HLAA NGH RCIR+LL++YIPS+    N  S + + + S +GFD   L +VIN+ ADG
Sbjct: 121 ALHLAALNGHPRCIRILLSEYIPSVP---NCWSLLKNKKTSVAGFDSSVLHEVINRAADG 180

Query: 181 GITALHMAALNGQVGSVQLLLDLGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           GIT LH+AALNG + +VQLLLDLGASV++VTV DGT IDLIGAGSTALHYA+CGGN +CC
Sbjct: 181 GITPLHVAALNGHIETVQLLLDLGASVTQVTVEDGTTIDLIGAGSTALHYASCGGNTQCC 240

Query: 241 QLLIARGASLTIENANGWTPLMVAHSWHKSWLEDILSSEPVGQPKLIL----SPYLSLPL 300
           QLLI++GA L   N+NGWTP+MVA SWH++WLE+IL +    QP+L L    SP+L LPL
Sbjct: 241 QLLISKGACLAAVNSNGWTPMMVARSWHRNWLEEIL-NPTTEQPQLHLPNVPSPFLCLPL 300

Query: 301 MSIVQIARDCGWSTKSAL-GCQDPCVVCLERKCAVAAEGCDHELCTRCALYLCSTN-CGS 360
           MSIV IA++CGW     L  C+DPC VCLERKC VAA+GC HE CT CALYL +T+   S
Sbjct: 301 MSIVNIAQECGWRENDCLTPCRDPCAVCLERKCTVAADGCAHEFCTNCALYLSTTSITSS 360

Query: 361 TTARGPPGSVSCPLCRNGIVSFVKLPEKSPVVKEIV--RTSLSLSSTCQCSHDSPLKSTS 420
            T+   PGSV CPLCRNGIVSF KLP  +   +     RTS+SL S C CS D  +  T+
Sbjct: 361 KTSNVTPGSVPCPLCRNGIVSFTKLPHTTATTRTSTSSRTSISL-SFCTCSSD--VLDTA 420

Query: 421 LTTQ-------LRTLELCRTHDSPLGLPFR---CQRFP 441
           L T        + +    RT  S     FR   C+RFP
Sbjct: 421 LLTNPHYSCKPVVSRTGSRTPQSARSSAFRSLSCRRFP 451

BLAST of MS002288 vs. ExPASy Swiss-Prot
Match: Q6KAE5 (Probable E3 ubiquitin-protein ligase XBOS32 OS=Oryza sativa subsp. japonica OX=39947 GN=XBOS32 PE=2 SV=2)

HSP 1 Score: 550.4 bits (1417), Expect = 2.0e-155
Identity = 285/459 (62.09%), Postives = 339/459 (73.86%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           M  LS V NSFGCSASGERLVSAARDGD QEA+ALLEYNPRLARYSTFG RNSPLHY+AA
Sbjct: 1   MGFLSLVGNSFGCSASGERLVSAARDGDLQEARALLEYNPRLARYSTFGGRNSPLHYAAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGHWEVVLILILFRASIHKADYLNGGT 120
            GHHEIV LLL+SGV+INLRNYRGQTALMQACQ+GHWEVV  L+LF A++H+ DYLNGG+
Sbjct: 61  QGHHEIVSLLLESGVEINLRNYRGQTALMQACQYGHWEVVQTLMLFNANVHRTDYLNGGS 120

Query: 121 AIHLAASNGHSRCIRLLLADYIPSIANFRNIMSKITDNEESTSGFDHRALSQVINQPADG 180
           A+H AA +GH+RC+RL+LADY+PS+ NF N M      E  ++  D   L +++NQ ADG
Sbjct: 121 ALHFAALHGHARCLRLVLADYVPSMPNFWNSMKDSLSEEGPSADLDEDGLFKMVNQKADG 180

Query: 181 GITALHMAALNGQVGSVQLLLDLGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           G+T LHMAALNG V  VQLLLDLGASV E T+ DGT IDLIGAGST LHYAACGGNA CC
Sbjct: 181 GLTPLHMAALNGHVECVQLLLDLGASVIEATIEDGTTIDLIGAGSTPLHYAACGGNAVCC 240

Query: 241 QLLIARGASLTIENANGWTPLMVAHSWHKSWLEDILSSEPVGQPKLILSPYLSLPLMSIV 300
           QLLIARGASL+ +NA+GWTPLMVA SWH++ LE+ILS EP  + + + SPYL LPLMSI+
Sbjct: 241 QLLIARGASLSAQNASGWTPLMVARSWHRNSLEEILSKEPESRIRTVPSPYLCLPLMSIM 300

Query: 301 QIARDCGWS-TKSALGCQDPCVVCLERKCAVAAEGCDHELCTRCALYLCSTNCGSTTARG 360
            IAR+ GW     +  C DPC VCLE  C+VAAEGC HE CTRCALYLCST   S T+  
Sbjct: 301 SIAREFGWRYLNQSPVCIDPCAVCLEGSCSVAAEGCKHEFCTRCALYLCST---SYTSVS 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPEKSPVVKEIVRTSLSLSSTCQCSHDSPLKSTSLTTQLRT 420
           P G++ CPLCR+ I++F  LP  SP ++E+ R SLSLS    C   +   + S+ + L  
Sbjct: 361 PAGAIPCPLCRHPIIAFTALPGTSP-IRELPRNSLSLSFCTTCPAVNSDSTPSIASHLYR 420

Query: 421 LELCRTHDSPLG-LPFR---CQRFPSIKFNSNLCRETPD 455
            E       P+G   FR   CQR P++K N + C    D
Sbjct: 421 TEFQCARMPPMGSSSFRSLSCQRLPAMKLNPSFCMGAMD 455

BLAST of MS002288 vs. ExPASy Swiss-Prot
Match: Q7EZ44 (Probable E3 ubiquitin-protein ligase XBOS35 OS=Oryza sativa subsp. japonica OX=39947 GN=XBOS35 PE=2 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 1.1e-142
Identity = 253/398 (63.57%), Postives = 310/398 (77.89%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           M LL  V +SFGCSA+GERLVSAARDGD QEA ALLE NPRLARYSTFG+RNSPLHYSAA
Sbjct: 1   MGLLGMVGDSFGCSATGERLVSAARDGDIQEAMALLELNPRLARYSTFGIRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGHWEVVLILILFRASIHKADYLNGGT 120
            GHHEIV LL++SGVDINLRN RGQTALMQAC +GHW+VV IL+LF+A+IHK D  +G T
Sbjct: 61  KGHHEIVSLLIESGVDINLRNCRGQTALMQACLYGHWKVVQILVLFKANIHKKDCFSGAT 120

Query: 121 AIHLAASNGHSRCIRLLLADYIPSIANFRNIMSKITDNEESTSGFDHRALSQVINQPADG 180
           AIH AA  GH+RC+RLL+ADY+PS+  F ++M     +E +   FD  AL ++IN  +DG
Sbjct: 121 AIHFAALKGHTRCLRLLVADYVPSLPEFWSVMHAKCTDETNKEAFDAVALRRLINNKSDG 180

Query: 181 GITALHMAALNGQVGSVQLLLDLGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           G+T LH+AAL+G    VQLLLDLGASVSEVT+ DG+ IDLIG+GST LHYAACGG+A CC
Sbjct: 181 GVTPLHLAALHGHAECVQLLLDLGASVSEVTINDGSTIDLIGSGSTPLHYAACGGSAVCC 240

Query: 241 QLLIARGASLTIENANGWTPLMVAHSWHKSWLEDILSSEPVGQPKLILSPYLSLPLMSIV 300
           QLL+A GA++  +N NG TPLMVA SWHKS +E IL+       +++ S YLSLPLMSIV
Sbjct: 241 QLLVAAGANMRAQNTNGLTPLMVARSWHKSSVEGILTKRSEVPVRILPSSYLSLPLMSIV 300

Query: 301 QIARDCGW-STKSALGCQDPCVVCLERKCAVAAEGCDHELCTRCALYLCSTNCGSTTARG 360
           +IAR+CGW  T  +  C DPC +CL+ +C V+AEGC HE CT+CALYLC+T   ST+ RG
Sbjct: 301 KIARECGWRKTSVSSVCHDPCAICLDTECTVSAEGCGHEFCTKCALYLCATASSSTSIRG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPEKSPVVKEIVRTSLSLS 398
            PGS+ CPLCR+ IVSFV+L   +P +KE+  T+ SL+
Sbjct: 361 VPGSIPCPLCRHTIVSFVRLTSTTP-IKELPWTNKSLA 397

BLAST of MS002288 vs. ExPASy Swiss-Prot
Match: Q4FE45 (E3 ubiquitin-protein ligase XBAT33 OS=Arabidopsis thaliana OX=3702 GN=XBAT33 PE=2 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 4.8e-122
Identity = 241/450 (53.56%), Postives = 302/450 (67.11%), Query Frame = 0

Query: 9   NSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAAHGHHEIVY 68
           NSFGCSASGERLVSAARDGDF EA+ LL+ NP LA+YSTFG  NSPLH++AA GH+EIV 
Sbjct: 3   NSFGCSASGERLVSAARDGDFVEAKMLLDCNPCLAKYSTFGGLNSPLHFAAAKGHNEIVG 62

Query: 69  LLLQSGVDINLRNYRGQTALMQACQHGHWEVVLILILFRASIHKADYLNGGTAIHLAASN 128
           LLL++G D+N RNY GQTALMQAC++GHWEVV  L+LFR ++ +ADYL G TA+H AA N
Sbjct: 63  LLLENGADVNSRNYCGQTALMQACRYGHWEVVQTLLLFRCNVTRADYLAGRTALHFAAVN 122

Query: 129 GHSRCIRLLLADYIPS-IANFRNIMSKITDNEESTSGFDHRALSQVINQPADGGITALHM 188
           GH+RCIRL+LAD++PS   N       +T   +S    +  ALS+ +N+ ADGGITALHM
Sbjct: 123 GHARCIRLVLADFLPSDKLNSLPETGVVTAKNKS----EQSALSKFVNKAADGGITALHM 182

Query: 189 AALNGQVGSVQLLLDLGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCCQLLIARG 248
           AALNG    VQLLLDL A+VS VT   GT +D+IGAGST LHYAACGGN KCCQ+L+ARG
Sbjct: 183 AALNGLFDCVQLLLDLEANVSAVTFHYGTSMDMIGAGSTPLHYAACGGNLKCCQILLARG 242

Query: 249 ASLTIENANGWTPLMVAHSWHKSWLEDILS-SEPVGQPKLILSPYLSLPLMSIVQIARDC 308
           A     N NGW P+ +A  W + WLE +LS +  V  P    S YLSLPL+SI+ IAR+ 
Sbjct: 243 ARKMTLNCNGWLPIDIARMWSRHWLEPLLSPNSDVVIPAFPHSNYLSLPLLSILNIAREF 302

Query: 309 GWSTKSALGCQDPCVVCLERKCAVAAEGCDHELCTRCALYLCSTNCGSTTARGPPGSVSC 368
           G  + +     D C VCLER C VAAEGC+H+LC RCALYLCS++   +   GPPGS+ C
Sbjct: 303 GLQSATIGDEVDICAVCLERTCTVAAEGCEHQLCVRCALYLCSSSNVPSVTVGPPGSIPC 362

Query: 369 PLCRNGIVSFVKLPEKSPVVKEI-VRTSLSLSSTCQC----SHDSPLKSTSLTTQLRTLE 428
           PLCR+GI +F +LP  S + +E+ +  SL   + C      + D    +   T Q  +  
Sbjct: 363 PLCRHGITAFKRLP--SSLTREMKLPMSLGFCAPCMLHTGDTTDQSSPTCPPTEQRSSKT 422

Query: 429 LCRTHDSPLGLPFRCQRFPSIKFNSNLCRE 452
              +  S +  P  C  FPS+      C E
Sbjct: 423 RAASVSSDIFCPVTCSPFPSVNIPMCTCNE 446

BLAST of MS002288 vs. ExPASy Swiss-Prot
Match: Q337A0 (Probable E3 ubiquitin-protein ligase XBOS33 OS=Oryza sativa subsp. japonica OX=39947 GN=XBOS33 PE=2 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 1.0e-116
Identity = 243/471 (51.59%), Postives = 307/471 (65.18%), Query Frame = 0

Query: 9   NSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAAHGHHEIVY 68
           NS GCSASGERLVSAARDGD  EA+ LLE +P LARYSTFG  NSPLH++AA GH +IV 
Sbjct: 3   NSLGCSASGERLVSAARDGDAVEARMLLELSPALARYSTFGGLNSPLHFAAAKGHLDIVT 62

Query: 69  LLLQSGVDINLRNYRGQTALMQACQHGHWEVVLILILFRASIHKADYLNGGTAIHLAASN 128
           LLL+ G D+N+RNY GQTALM AC+HGHWEVV +L+LFR ++ +ADYL+G TA+H AA +
Sbjct: 63  LLLEKGADVNVRNYCGQTALMHACRHGHWEVVQMLLLFRCNVTRADYLSGRTALHFAAHD 122

Query: 129 GHSRCIRLLLADYIPSIANFRNIMSKITDNE-ESTSG----------FDHRALSQVINQP 188
           G  RC+RLLLAD++PS        S +   E ++ SG          F+  A  + IN+P
Sbjct: 123 GLVRCVRLLLADFVPSAPLEDGASSTVDGGECQTNSGSSPCSSLGLKFNESARLRYINKP 182

Query: 189 ADGGITALHMAALNGQVGSVQLLLDLGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNA 248
           ADGG+TALHMAALNG    +QLL+DLGA+VS VT   GT  +LIGAGST LHYAA GGNA
Sbjct: 183 ADGGVTALHMAALNGHFDCMQLLIDLGANVSAVTFPYGTTANLIGAGSTPLHYAAGGGNA 242

Query: 249 KCCQLLIARGASLTIENANGWTPLMVAHSWHKSWLEDIL-----SSEPVGQPKLILSPYL 308
           +CCQLL+++GAS    N NGW P+ VA  + + +LE +L     SS P  QP    S YL
Sbjct: 243 ECCQLLLSKGASKLTLNCNGWLPIDVARMFGRRFLEPLLSPNSNSSIPAYQP----SNYL 302

Query: 309 SLPLMSIVQIARDCG-WSTKSALGCQDPCVVCLERKCAVAAEGCDHELCTRCALYLCSTN 368
           +LP MSI+ IAR+ G   T +++   D C VCLER C+VAAEGC HE C +CALYLCST+
Sbjct: 303 ALPFMSILNIAREFGLLHTVASVDDSDLCAVCLERSCSVAAEGCCHEFCIKCALYLCSTS 362

Query: 369 CGSTTARGPPGSVSCPLCRNGIVSFVKLPEKSPVVKEIVRTSLSLSSTCQC---SHDSPL 428
                  GPPGS+ CPLCRNGI+SF KLP  +P       ++L+  + C     S DSP 
Sbjct: 363 NTRVEFTGPPGSIPCPLCRNGIMSFTKLP-STPTEGLKSSSALTFCNPCMLNTRSVDSP- 422

Query: 429 KSTSLTTQLRTLELCRTHDSPLGLPFRCQRFPSIKFNSNLCRETPDICHSL 460
            +T    ++R   +     S L  P  C  FPS    +  C +  D C ++
Sbjct: 423 -ATISKAEIRRNRVAAV-SSELVCPLTCSPFPSSALPTCRCSD-DDPCDAI 464

BLAST of MS002288 vs. ExPASy TrEMBL
Match: A0A6J1C0R4 (E3 ubiquitin-protein ligase XBAT32 OS=Momordica charantia OX=3673 GN=LOC111007008 PE=4 SV=1)

HSP 1 Score: 966.8 bits (2498), Expect = 3.2e-278
Identity = 474/477 (99.37%), Postives = 476/477 (99.79%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGHWEVVLILILFRASIHKADYLNGGT 120
           HGHHEIVYLLLQSGVD+NLRNYRGQTALMQACQHGHWEVVLIL+LFRASIHKADYLNGGT
Sbjct: 61  HGHHEIVYLLLQSGVDVNLRNYRGQTALMQACQHGHWEVVLILVLFRASIHKADYLNGGT 120

Query: 121 AIHLAASNGHSRCIRLLLADYIPSIANFRNIMSKITDNEESTSGFDHRALSQVINQPADG 180
           AIHLAASNGHSRCIRLLLADYIPSIANFRNIMSKITDNEESTSGFDHRALSQVINQPADG
Sbjct: 121 AIHLAASNGHSRCIRLLLADYIPSIANFRNIMSKITDNEESTSGFDHRALSQVINQPADG 180

Query: 181 GITALHMAALNGQVGSVQLLLDLGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           GITALHMAALNGQVGSVQLLLDLGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCC
Sbjct: 181 GITALHMAALNGQVGSVQLLLDLGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCC 240

Query: 241 QLLIARGASLTIENANGWTPLMVAHSWHKSWLEDILSSEPVGQPKLILSPYLSLPLMSIV 300
           QLLIARGASLTIENANGWTPLMVAHSWHKSWLEDILSSEPVGQPKLILSPYLSLPLMSIV
Sbjct: 241 QLLIARGASLTIENANGWTPLMVAHSWHKSWLEDILSSEPVGQPKLILSPYLSLPLMSIV 300

Query: 301 QIARDCGWSTKSALGCQDPCVVCLERKCAVAAEGCDHELCTRCALYLCSTNCGSTTARGP 360
           QIARDCGWSTKSALGCQDPCVVCLERKCAVAAEGCDHE CTRCALYLCSTNCGSTTARGP
Sbjct: 301 QIARDCGWSTKSALGCQDPCVVCLERKCAVAAEGCDHEFCTRCALYLCSTNCGSTTARGP 360

Query: 361 PGSVSCPLCRNGIVSFVKLPEKSPVVKEIVRTSLSLSSTCQCSHDSPLKSTSLTTQLRTL 420
           PGSVSCPLCRNGIVSFVKLPEKSPVVKEIVRTSLSLSSTCQCSHDSPLKSTSLTTQLRTL
Sbjct: 361 PGSVSCPLCRNGIVSFVKLPEKSPVVKEIVRTSLSLSSTCQCSHDSPLKSTSLTTQLRTL 420

Query: 421 ELCRTHDSPLGLPFRCQRFPSIKFNSNLCRETPDICHSLVPRYAHWNLRNHLARCSR 478
           ELCRTHDSPLGLPFRCQRFPSIKFNSNLCRETPDICHSLVPRYAHWNLRNHLARCSR
Sbjct: 421 ELCRTHDSPLGLPFRCQRFPSIKFNSNLCRETPDICHSLVPRYAHWNLRNHLARCSR 477

BLAST of MS002288 vs. ExPASy TrEMBL
Match: A0A5D3B9Z5 (E3 ubiquitin-protein ligase XBAT32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold271G00090 PE=4 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 1.9e-225
Identity = 396/476 (83.19%), Postives = 429/476 (90.13%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MK LSAVV+SFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKFLSAVVHSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGHWEVVLILILFRASIHKADYLNGGT 120
           HGHHEIVYLLL SGVDINLRNYRGQTALMQACQHGHWEVVLIL+LF A++HKADYLNG T
Sbjct: 61  HGHHEIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWT 120

Query: 121 AIHLAASNGHSRCIRLLLADYIPSIANFRNIMSK-ITDNEESTSGFDHRALSQVINQPAD 180
           A+HLAA+NGHSRCIRLLLADYIPSI NFR IMS+  TD+EES S FDHRALSQ+INQ AD
Sbjct: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSRQSTDSEESISEFDHRALSQIINQKAD 180

Query: 181 GGITALHMAALNGQVGSVQLLLDLGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240
           GGITALHMAALNGQV SVQLLL+ GASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC
Sbjct: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240

Query: 241 CQLLIARGASLTIENANGWTPLMVAHSWHKSWLEDILSSEPVGQPKLILSPYLSLPLMSI 300
           CQLLIARGASLT EN NGWTPLMV+ SW+KSWLEDIL++EPV +PKLI S Y+SLPLMSI
Sbjct: 241 CQLLIARGASLTTENENGWTPLMVSRSWNKSWLEDILTTEPVERPKLIPSRYISLPLMSI 300

Query: 301 VQIARDCGWSTKSALGCQDPCVVCLERKCAVAAEGCDHELCTRCALYLCSTNCGSTTARG 360
           VQIAR+ GWS+KSA GCQDPCVVCLERKC VAA GCDHE CTRCALYLCSTNCG+T +RG
Sbjct: 301 VQIAREYGWSSKSAPGCQDPCVVCLERKCTVAAGGCDHEFCTRCALYLCSTNCGTTISRG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPEKSPVVKEIVRTSLSLSSTCQCSHDSPLKSTSLTTQLRT 420
           PPGSVSCPLCRNGIVSFVKLP K+ + KEI RTSLS    CQCSH++PLK T LTTQLR+
Sbjct: 361 PPGSVSCPLCRNGIVSFVKLPGKA-LAKEIARTSLSF-PICQCSHETPLKPTPLTTQLRS 420

Query: 421 LELCRTHDSPLGLPFRCQRFPSIKFNSNLCRETPDICHSLVPRYAHWNLRNHLARC 476
           LELCR HD+PLG+ F C + PSI    +LCR+ PDICHSLVPR A+ NL+NHL+RC
Sbjct: 421 LELCRVHDTPLGIYFCCHKLPSI----SLCRKAPDICHSLVPRCANGNLQNHLSRC 470

BLAST of MS002288 vs. ExPASy TrEMBL
Match: A0A5A7SV55 (E3 ubiquitin-protein ligase XBAT32 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold57G002150 PE=4 SV=1)

HSP 1 Score: 788.9 bits (2036), Expect = 1.2e-224
Identity = 395/476 (82.98%), Postives = 428/476 (89.92%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MK LSAVV+SFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKFLSAVVHSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGHWEVVLILILFRASIHKADYLNGGT 120
           HGHHEIVYLLL SGVDINLRNYRGQTALMQACQHGHWEVVLIL+LF A++HKADYLNG T
Sbjct: 61  HGHHEIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWT 120

Query: 121 AIHLAASNGHSRCIRLLLADYIPSIANFRNIMSK-ITDNEESTSGFDHRALSQVINQPAD 180
           A+HLAA+NGHSRCIRLLLADYIPSI NFR IMS+  TD+EES S FDHRALSQ+INQ AD
Sbjct: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSRQSTDSEESISEFDHRALSQIINQKAD 180

Query: 181 GGITALHMAALNGQVGSVQLLLDLGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240
           GGITALHMAALNGQV SVQLLL+ GASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC
Sbjct: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240

Query: 241 CQLLIARGASLTIENANGWTPLMVAHSWHKSWLEDILSSEPVGQPKLILSPYLSLPLMSI 300
           CQLLIARGASLT EN NGWTPLMV+ SW+KSWLEDIL++EPV +PKLI S Y+SLPLMSI
Sbjct: 241 CQLLIARGASLTTENENGWTPLMVSRSWNKSWLEDILTTEPVERPKLIPSRYISLPLMSI 300

Query: 301 VQIARDCGWSTKSALGCQDPCVVCLERKCAVAAEGCDHELCTRCALYLCSTNCGSTTARG 360
           VQIAR+ GWS+KSA GCQDPCVVCLERKC VAA GCDHE CTRCALYLCSTNCG+T +RG
Sbjct: 301 VQIAREYGWSSKSAPGCQDPCVVCLERKCTVAAGGCDHEFCTRCALYLCSTNCGTTISRG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPEKSPVVKEIVRTSLSLSSTCQCSHDSPLKSTSLTTQLRT 420
           PPGSVSCPLCRNGIVSFVKLP K+ + KEI RTSLS    CQCSH++P K T LTTQLR+
Sbjct: 361 PPGSVSCPLCRNGIVSFVKLPGKA-LAKEIARTSLSF-PICQCSHETPPKPTPLTTQLRS 420

Query: 421 LELCRTHDSPLGLPFRCQRFPSIKFNSNLCRETPDICHSLVPRYAHWNLRNHLARC 476
           LELCR HD+PLG+ F C + PSI    +LCR+ PDICHSLVPR A+ NL+NHL+RC
Sbjct: 421 LELCRVHDTPLGIYFCCHKLPSI----SLCRKAPDICHSLVPRCANGNLQNHLSRC 470

BLAST of MS002288 vs. ExPASy TrEMBL
Match: A0A1S3B8E1 (E3 ubiquitin-protein ligase XBAT32 OS=Cucumis melo OX=3656 GN=LOC103487302 PE=4 SV=1)

HSP 1 Score: 788.9 bits (2036), Expect = 1.2e-224
Identity = 395/476 (82.98%), Postives = 428/476 (89.92%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MK LSAVV+SFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKFLSAVVHSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGHWEVVLILILFRASIHKADYLNGGT 120
           HGHHEIVYLLL SGVDINLRNYRGQTALMQACQHGHWEVVLIL+LF A++HKADYLNG T
Sbjct: 61  HGHHEIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWT 120

Query: 121 AIHLAASNGHSRCIRLLLADYIPSIANFRNIMSK-ITDNEESTSGFDHRALSQVINQPAD 180
           A+HLAA+NGHSRCIRLLLADYIPSI NFR IMS+  TD+EES S FDHRALSQ+INQ AD
Sbjct: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSRQSTDSEESISEFDHRALSQIINQKAD 180

Query: 181 GGITALHMAALNGQVGSVQLLLDLGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240
           GGITALHMAALNGQV SVQLLL+ GASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC
Sbjct: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240

Query: 241 CQLLIARGASLTIENANGWTPLMVAHSWHKSWLEDILSSEPVGQPKLILSPYLSLPLMSI 300
           CQLLIARGASLT EN NGWTPLMV+ SW+KSWLEDIL++EPV +PKLI S Y+SLPLMSI
Sbjct: 241 CQLLIARGASLTTENENGWTPLMVSRSWNKSWLEDILTTEPVERPKLIPSRYISLPLMSI 300

Query: 301 VQIARDCGWSTKSALGCQDPCVVCLERKCAVAAEGCDHELCTRCALYLCSTNCGSTTARG 360
           VQIAR+ GWS+KSA GCQDPCVVCLERKC VAA GCDHE CTRCALYLCSTNCG+T +RG
Sbjct: 301 VQIAREYGWSSKSAPGCQDPCVVCLERKCTVAAGGCDHEFCTRCALYLCSTNCGTTISRG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPEKSPVVKEIVRTSLSLSSTCQCSHDSPLKSTSLTTQLRT 420
           PPGSVSCPLCRNGIVSFVKLP K+ + KEI RTSLS    CQCSH++P K T LTTQLR+
Sbjct: 361 PPGSVSCPLCRNGIVSFVKLPGKA-LAKEIARTSLSF-PICQCSHETPPKPTPLTTQLRS 420

Query: 421 LELCRTHDSPLGLPFRCQRFPSIKFNSNLCRETPDICHSLVPRYAHWNLRNHLARC 476
           LELCR HD+PLG+ F C + PSI    +LCR+ PDICHSLVPR A+ NL+NHL+RC
Sbjct: 421 LELCRVHDTPLGIYFCCHKLPSI----SLCRKAPDICHSLVPRCANGNLQNHLSRC 470

BLAST of MS002288 vs. ExPASy TrEMBL
Match: A0A0A0LWB1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G294100 PE=4 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 1.9e-222
Identity = 392/476 (82.35%), Postives = 424/476 (89.08%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           MK LSAVV+SFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MKFLSAVVHSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGHWEVVLILILFRASIHKADYLNGGT 120
           HGHHEIVYLLL SGVDINLRNYRGQTALMQACQHGHWEVVLIL+LF A++HKADYLNG T
Sbjct: 61  HGHHEIVYLLLHSGVDINLRNYRGQTALMQACQHGHWEVVLILVLFGANVHKADYLNGWT 120

Query: 121 AIHLAASNGHSRCIRLLLADYIPSIANFRNIMSK-ITDNEESTSGFDHRALSQVINQPAD 180
           A+HLAA+NGHSRCIRLLLADYIPSI NFR IMS+ +TDNEES S FDHRALSQ+INQ AD
Sbjct: 121 ALHLAATNGHSRCIRLLLADYIPSIPNFREIMSRQLTDNEESISEFDHRALSQIINQKAD 180

Query: 181 GGITALHMAALNGQVGSVQLLLDLGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240
           GGITALHMAALNGQV SVQLLL+ GASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC
Sbjct: 181 GGITALHMAALNGQVESVQLLLEFGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKC 240

Query: 241 CQLLIARGASLTIENANGWTPLMVAHSWHKSWLEDILSSEPVGQPKLILSPYLSLPLMSI 300
           CQLLIARGASLT EN NGWTPLMV+ SW+K+WLEDIL++EPV +PKLI S Y+SLPLMSI
Sbjct: 241 CQLLIARGASLTTENENGWTPLMVSRSWNKNWLEDILTTEPVERPKLIPSRYISLPLMSI 300

Query: 301 VQIARDCGWSTKSALGCQDPCVVCLERKCAVAAEGCDHELCTRCALYLCSTNCGSTTARG 360
           VQIAR+ GW++KSA GCQDPCVVCLERKC VAAEGCDHE CTRCALYLCSTNCG+T +RG
Sbjct: 301 VQIAREYGWTSKSAPGCQDPCVVCLERKCTVAAEGCDHEFCTRCALYLCSTNCGTTISRG 360

Query: 361 PPGSVSCPLCRNGIVSFVKLPEKSPVVKEIVRTSLSLSSTCQCSHDSPLKSTSLTTQLRT 420
           PPGSVSCPLCRNGIVSF KL  K+ + KEI RT LS     QCS ++P K T LTTQLR+
Sbjct: 361 PPGSVSCPLCRNGIVSFNKLRSKA-LAKEIARTRLSFPIR-QCSQETPSKPTPLTTQLRS 420

Query: 421 LELCRTHDSPLGLPFRCQRFPSIKFNSNLCRETPDICHSLVPRYAHWNLRNHLARC 476
           LELCR HD+PLG+ F C R PSI    NLCR+TPDICHSLVPR A+ N +NHL RC
Sbjct: 421 LELCRVHDTPLGIYFCCHRLPSI----NLCRKTPDICHSLVPRCANGNFQNHLGRC 470

BLAST of MS002288 vs. TAIR 10
Match: AT5G57740.1 (XB3 ortholog 2 in Arabidopsis thaliana )

HSP 1 Score: 552.0 bits (1421), Expect = 4.8e-157
Identity = 295/458 (64.41%), Postives = 346/458 (75.55%), Query Frame = 0

Query: 1   MKLLSAVVNSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAA 60
           M+ LS V NSFGCSASGERLVSAARDGD QEA+ALL+YNPRLARYSTFGVRNSPLHYSAA
Sbjct: 1   MRFLSLVGNSFGCSASGERLVSAARDGDLQEAKALLDYNPRLARYSTFGVRNSPLHYSAA 60

Query: 61  HGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGHWEVVLILILFRASIHKADYLNGGT 120
            GHHEIV LL++SGVDINLRNYRGQTALMQACQHGHWEVVLILILF A+IH++DYLNGGT
Sbjct: 61  QGHHEIVSLLVESGVDINLRNYRGQTALMQACQHGHWEVVLILILFGANIHRSDYLNGGT 120

Query: 121 AIHLAASNGHSRCIRLLLADYIPSIANFRNIMSKITDNEESTSGFDHRALSQVINQPADG 180
           A+HLAA NGH RCIR+LL++YIPS+    N  S + + + S +GFD   L +VIN+ ADG
Sbjct: 121 ALHLAALNGHPRCIRILLSEYIPSVP---NCWSLLKNKKTSVAGFDSSVLHEVINRAADG 180

Query: 181 GITALHMAALNGQVGSVQLLLDLGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCC 240
           GIT LH+AALNG + +VQLLLDLGASV++VTV DGT IDLIGAGSTALHYA+CGGN +CC
Sbjct: 181 GITPLHVAALNGHIETVQLLLDLGASVTQVTVEDGTTIDLIGAGSTALHYASCGGNTQCC 240

Query: 241 QLLIARGASLTIENANGWTPLMVAHSWHKSWLEDILSSEPVGQPKLIL----SPYLSLPL 300
           QLLI++GA L   N+NGWTP+MVA SWH++WLE+IL +    QP+L L    SP+L LPL
Sbjct: 241 QLLISKGACLAAVNSNGWTPMMVARSWHRNWLEEIL-NPTTEQPQLHLPNVPSPFLCLPL 300

Query: 301 MSIVQIARDCGWSTKSAL-GCQDPCVVCLERKCAVAAEGCDHELCTRCALYLCSTN-CGS 360
           MSIV IA++CGW     L  C+DPC VCLERKC VAA+GC HE CT CALYL +T+   S
Sbjct: 301 MSIVNIAQECGWRENDCLTPCRDPCAVCLERKCTVAADGCAHEFCTNCALYLSTTSITSS 360

Query: 361 TTARGPPGSVSCPLCRNGIVSFVKLPEKSPVVKEIV--RTSLSLSSTCQCSHDSPLKSTS 420
            T+   PGSV CPLCRNGIVSF KLP  +   +     RTS+SL S C CS D  +  T+
Sbjct: 361 KTSNVTPGSVPCPLCRNGIVSFTKLPHTTATTRTSTSSRTSISL-SFCTCSSD--VLDTA 420

Query: 421 LTTQ-------LRTLELCRTHDSPLGLPFR---CQRFP 441
           L T        + +    RT  S     FR   C+RFP
Sbjct: 421 LLTNPHYSCKPVVSRTGSRTPQSARSSAFRSLSCRRFP 451

BLAST of MS002288 vs. TAIR 10
Match: AT5G07270.1 (XB3 ortholog 3 in Arabidopsis thaliana )

HSP 1 Score: 439.5 bits (1129), Expect = 3.4e-123
Identity = 241/450 (53.56%), Postives = 302/450 (67.11%), Query Frame = 0

Query: 9   NSFGCSASGERLVSAARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAAHGHHEIVY 68
           NSFGCSASGERLVSAARDGDF EA+ LL+ NP LA+YSTFG  NSPLH++AA GH+EIV 
Sbjct: 3   NSFGCSASGERLVSAARDGDFVEAKMLLDCNPCLAKYSTFGGLNSPLHFAAAKGHNEIVG 62

Query: 69  LLLQSGVDINLRNYRGQTALMQACQHGHWEVVLILILFRASIHKADYLNGGTAIHLAASN 128
           LLL++G D+N RNY GQTALMQAC++GHWEVV  L+LFR ++ +ADYL G TA+H AA N
Sbjct: 63  LLLENGADVNSRNYCGQTALMQACRYGHWEVVQTLLLFRCNVTRADYLAGRTALHFAAVN 122

Query: 129 GHSRCIRLLLADYIPS-IANFRNIMSKITDNEESTSGFDHRALSQVINQPADGGITALHM 188
           GH+RCIRL+LAD++PS   N       +T   +S    +  ALS+ +N+ ADGGITALHM
Sbjct: 123 GHARCIRLVLADFLPSDKLNSLPETGVVTAKNKS----EQSALSKFVNKAADGGITALHM 182

Query: 189 AALNGQVGSVQLLLDLGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCCQLLIARG 248
           AALNG    VQLLLDL A+VS VT   GT +D+IGAGST LHYAACGGN KCCQ+L+ARG
Sbjct: 183 AALNGLFDCVQLLLDLEANVSAVTFHYGTSMDMIGAGSTPLHYAACGGNLKCCQILLARG 242

Query: 249 ASLTIENANGWTPLMVAHSWHKSWLEDILS-SEPVGQPKLILSPYLSLPLMSIVQIARDC 308
           A     N NGW P+ +A  W + WLE +LS +  V  P    S YLSLPL+SI+ IAR+ 
Sbjct: 243 ARKMTLNCNGWLPIDIARMWSRHWLEPLLSPNSDVVIPAFPHSNYLSLPLLSILNIAREF 302

Query: 309 GWSTKSALGCQDPCVVCLERKCAVAAEGCDHELCTRCALYLCSTNCGSTTARGPPGSVSC 368
           G  + +     D C VCLER C VAAEGC+H+LC RCALYLCS++   +   GPPGS+ C
Sbjct: 303 GLQSATIGDEVDICAVCLERTCTVAAEGCEHQLCVRCALYLCSSSNVPSVTVGPPGSIPC 362

Query: 369 PLCRNGIVSFVKLPEKSPVVKEI-VRTSLSLSSTCQC----SHDSPLKSTSLTTQLRTLE 428
           PLCR+GI +F +LP  S + +E+ +  SL   + C      + D    +   T Q  +  
Sbjct: 363 PLCRHGITAFKRLP--SSLTREMKLPMSLGFCAPCMLHTGDTTDQSSPTCPPTEQRSSKT 422

Query: 429 LCRTHDSPLGLPFRCQRFPSIKFNSNLCRE 452
              +  S +  P  C  FPS+      C E
Sbjct: 423 RAASVSSDIFCPVTCSPFPSVNIPMCTCNE 446

BLAST of MS002288 vs. TAIR 10
Match: AT5G14230.1 (CONTAINS InterPro DOMAIN/s: Ankyrin repeat-containing domain (InterPro:IPR020683), Ankyrin repeat (InterPro:IPR002110); BEST Arabidopsis thaliana protein match is: XB3 ortholog 2 in Arabidopsis thaliana (TAIR:AT5G57740.1); Has 66374 Blast hits to 25358 proteins in 1201 species: Archae - 121; Bacteria - 8133; Metazoa - 29530; Fungi - 5885; Plants - 3349; Viruses - 785; Other Eukaryotes - 18571 (source: NCBI BLink). )

HSP 1 Score: 77.8 bits (190), Expect = 2.6e-14
Identity = 70/249 (28.11%), Postives = 114/249 (45.78%), Query Frame = 0

Query: 49  GVRNSPLHYSAAHGHHEIVYLLLQSGVDINLRNYRGQTALMQACQHGHWEVVLILILFRA 108
           G+   P+H +A  G  EI+  L+  G DIN +N  G TAL+ + +H H E V +L L  A
Sbjct: 339 GIELRPIHIAARDGSVEIIQQLVGFGCDINSKNDVGNTALLISIKHKHPECVKVLALDGA 398

Query: 109 SIHKADYLNGGTAIHLAASN----GHSRCIRLLLADYIPSIANFRNIMSKITDNEESTSG 168
                +   G +A+ +A SN    G  R I  L+   +   ++  ++ S +    ++   
Sbjct: 399 DFGLVNKF-GHSAVSIAESNKWSLGLERVILELIRFGVVPHSSNASVFSPLLYGAQAGDA 458

Query: 169 FDHRALSQV----INQPADGGITALHMAALNGQVGSVQLLLDLGASVSEVTVGDGTVIDL 228
              +AL +     ++   + G +A  +AA+NG V + ++L+  GA V        TV+ L
Sbjct: 459 EALKALVKAQDIYLDYQDEEGFSAAMLAAMNGHVEAFRVLVYAGADVKLYNNSGDTVVSL 518

Query: 229 -------------------------IGAGSTALHYAACGGNAKCCQLLIARGASLTIENA 265
                                    +  G  ALH AA  G+ K  +LL  +G SL I + 
Sbjct: 519 SEQNGNRDVIEKVMLEFALEKDSRNMAGGFYALHCAARRGDVKAVKLLSGKGYSLDIPDG 578

BLAST of MS002288 vs. TAIR 10
Match: AT5G02620.1 (ankyrin-like1 )

HSP 1 Score: 65.1 bits (157), Expect = 1.8e-10
Identity = 59/208 (28.37%), Postives = 90/208 (43.27%), Query Frame = 0

Query: 23  AARDGDFQEAQALLEYNPRLARYSTFGVRNSPLHYSAAHGHHEIVYLLLQSGVDI-NLRN 82
           AA++G+ Q    L+E NP L+ ++    + + LH +A+ GH EIV  LL  GVD+  +  
Sbjct: 98  AAKNGNLQVLDVLIEANPELS-FTFDSSKTTALHTAASQGHGEIVCFLLDKGVDLAAIAR 157

Query: 83  YRGQTALMQACQHGHWEVVLILILFRASIHKADYLNGGTAIHLAASNGHSRCIRLLLADY 142
             G+TAL  A ++GH  +V  LI  +A +       G TA+H+A    ++  + +L+   
Sbjct: 158 SNGKTALHSAARNGHTVIVKKLIEKKAGMVTRVDKKGQTALHMAVKGQNTEIVDVLM--- 217

Query: 143 IPSIANFRNIMSKITDNEESTSGFDHRALSQVINQPADGGITALHMAALNGQVGSVQLLL 202
                                      A   +IN   + G T LH+A    +   VQ +L
Sbjct: 218 --------------------------EADGSLINSADNKGNTPLHIAVRKNRAEIVQTVL 274

Query: 203 DLGASVSEVTVGDGTVIDLIGAGSTALH 230
                VS V V       L  A  T LH
Sbjct: 278 KY-CEVSRVAVNKSGETALDIAEKTGLH 274

BLAST of MS002288 vs. TAIR 10
Match: AT5G37500.1 (gated outwardly-rectifying K+ channel )

HSP 1 Score: 62.0 bits (149), Expect = 1.5e-09
Identity = 64/224 (28.57%), Postives = 97/224 (43.30%), Query Frame = 0

Query: 22  SAARDGDFQEAQALLE--YNPRLARYSTFGVRNSPLHYSAAHGHHEIVYLLLQSGVDINL 81
           SAA  GDF + ++L+    +P    Y       SPLH +A  G+ +I   L+Q GVD+NL
Sbjct: 537 SAAFQGDFYQLKSLIRSGADPNKTDYD----GRSPLHLAACRGYEDITLFLIQEGVDVNL 596

Query: 82  RNYRGQTALMQACQHGHWEVVLILILFRASIHKADYLNGGTAIHLAASNGHSRCIRLLLA 141
           ++  G T L +A + G   V+ +L+   AS +  D    G  +    + G S  ++ LL+
Sbjct: 597 KDKFGHTPLFEAVKAGQEGVIGLLVKEGASFNLED---SGNFLCTTVAKGDSDFLKRLLS 656

Query: 142 DYIPSIANFRNIMSKITDNEESTSGFDHRALSQVINQPADGGITALHMAALNGQVGSVQL 201
                        S +  N E    +DHR              T LH+AA  G     ++
Sbjct: 657 -------------SGMNPNSED---YDHR--------------TPLHVAASEGLFLMAKM 713

Query: 202 LLDLGASVSEVTVGDGTVIDLIGAGSTALHYAACGGNAKCCQLL 244
           L++ GAS          VI     G++ L  A   GN K  +LL
Sbjct: 717 LVEAGAS----------VISKDRWGNSPLDEARLCGNKKLIKLL 713