MS002280 (gene) Bitter gourd (TR) v1

Overview


Name	MS002280
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Diacylglycerol kinase
Location	scaffold30: 3806108 .. 3808949 (-)
RNA-Seq Expression	MS002280
Synteny	MS002280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCCACAGCCGACGACGTTACTATTGCTGCTCGCTCCTCCTTAATCGACTCCTTGAGGGGCTGTGGCTTGTCCGGGATCCCAATCGACAAGGCCCACCTCAAGGCCAACCTCATCATGCCGCAGTATCTCCGCTTGGCCCTGCGGGATTCAATTCGCTTCAAAGATCCCACCGCCAAAATCACTACGCCGGAGAACGTCCTGCCGCCGGAGGCTCCTCTGGTCGTCTTCATTAATCCGCGTAGCGGTGGACGCCATGGCCAGAAGCTCAAGCAGCGCCTCCAGCACTTAATCAGCGACGAACAGGTCTATTGTTTTGTTTGTTTCACTACTTGCTATGAAATTGCGCATTCCGATACCCCTTTTGTCTAAAAATCAAAATTTGCCCCAAACTTGGAAAAGTAGACTTTTTGACCTTTTTGTTCACGTGTGGCCGTACGTGTCGGGGTTGGAACTTGGATCTTCCTCTGTTTTTCGTGGATATTTTTTTGAGTTCAACGATTGTGGAGTGAGCCCTAAATTTAAGGGAGGTTATTGATTGACGTCTTCTTACCGTCTTCTTGTTAGTTCACTGAGTCAGCATCGCAACTCCAATGCGCGTGTCGCGTCTTGTTTGTTGTTTAAATATTTCAACCAATTAAAAAAAATATAAATAAATAAGATTCTTCAGGCTACGCTTACGACGGTATTATTTTTAATTTTGTATTGAAAAAGTTGGAATTTGGTGTATTGGGCAATAAAATTTGACGCTTTTGCTACAGAGAGTGGATTAGATTGACGTTAAAGTTTTGTGTTTGTTGGTTGCAGGTTTTCGATCTTTTGGATGTGAAGCCTCATGAATTTGTGCAGTACGGACTGCGTTGCCTGGAAATCTTGTCTGATCTTGGCGACGCTTGTGCCAAACAAACTCGTCAAAAGTTAAGGGTCATGGTACGTTTCTTTATTTCTCCTTTCCACATTTTTGAATTAATTCTATTAGAAAAAGAAGGAAAAAAAACATGAACTGGGCAGGTGGCAGGAGGTGATGGCACTGTCGGTTGGGTACTGGGAAGTCTTGCAGAACTTCACAAACAAGATCGCAGCCCTGTTCTTCCAGTTGGAATTATCCCTCTGGGTACTGGAAATGACCTCTCCAGGAGTTTTGGCTGGGTGAGTTTTTGTAATTTCAACACAAATTTTTGCATGAAGTGATCATCGTTTATTTCAATGGACATTGGTTTTTGGTTTGATCAGGGAGGTTCATTTCCTTTTGCCTGGAAATACGCCGTTAAAAGATCTCTCCACAGAGCTACCTCTGGCCAAGTTCGTTCTTTGGATAGGTGGGAATTCCAATTCTTTCAATGGCATTGCTTCTTGAATAGTAAAAGGCGTTTTTGAGTTGTTTCAAACTTGCTATTCTCACGGCGCAGTTGGCATGTTTCGCTGTCCATGCCTGCTGGAGAAAAAGTGGAACCACCACATTCTCTGAAGCAGGTTACACAAGAAAGTGATCTGAATGAGCCTCCTGTTCATGTAAATTATTTACATTTTCACACTACAAATTTTGCATTATATTCTCATTTCAGTCACGATTCAAAATCCATAATCCCATGGCTGCAGGATGACAAAGGAGATTTACCAACAAAACTGGCTTGCTATGAAGGAGTGTTTTACAATTACTTGAGTATAGGTAGGCTGTACTCACTTGTGTCTGCATGATCAATCAAACTGTAGAGTATTCGTTCACAGGTTTAATTAATTGATTCTACTATTTCACAATGCAGGAATGGATGCTCAAGTTGCTTACGGCTTCCACCATTTGCGTAACAAAAGACCTTACCTTGCTCAAGGTCCAGTGACAAATAAGGTCCAGACCATCTGTTTAGCCATTGACGAAAGAATTGCATTCAGGACTAGTGGTTGTTTTGTTGTTTAATAAGCTTACACGTGCTTTCTTTTGTTTGTGACAGCTGATTTACTCTGGTTATAGTTGCACTCAAGGTTGGTTCTTCACATCCTGCTCAAAAGATCCAAATTTGAGGTGATAATATTTATATTTAATGAAAACCCAGTAGAATACATCGGTTTTCTTCCAGTTTCTAAGCACAGAAAGCTTCTCTGGTTGTAGGGGCCTTAAGAACATCCTTAGAATACACATCAAAAAGGTCAATTGCTCGGACTGGGAAAAGATTCCAGTTCCTTCCAGGTAGAATTACTCTAGTAACAGTACAGTAACCATGAAACTATGCTTTTATGTATAAATTTAAGCTTGTTGAGGACATTAATTGATGTTCTTGTTTCGATTGGCTGTAGTGTTAGGTCTATTGTTGCTTTAAACCTACATAACTATGCAAGTGGAAGAAACCCATGGGGTAAGCCCAAGCCAGAGTACTTGGAGAAGGTACCCACTAAAATTTCCCTTGATGAACAAGTTGTTGTTCTGTAATTTGCTTCTTTTTGGCATTTGATATTTATTTGGTAGCAGAGAGGGTTTGTGGAGGCACATGCTGATGATGGTCTTCTAGAGATTTTTGGGCTAAAACAAGGATGGCACACATCATTTGTTATGGTGGATATAATCTCTGCCAAACACATCGCCCAGGTACTTCAAATTAAACGCATTCAGACATAGTTCTTCTCTTGAAGTTGAATCTAACTCCACGATTCTAGGGTGTTTGATTGAACTGTTGCATAATATTTTCAGGCAGCAGCCATTAGAATTGAAATTAGAGGTGGGAAGCGGAAGAAGGCATATCTGCAGATGGATGGCGAGCCATGGAAGCAGCAAATAAGCAAGGACTCCTCGACGTTCGTTGAAATCAAGAGGGTTCCTTTCCAATCACTAATGATCAACGGGGAC

mRNA sequence

ATGGATTCCACAGCCGACGACGTTACTATTGCTGCTCGCTCCTCCTTAATCGACTCCTTGAGGGGCTGTGGCTTGTCCGGGATCCCAATCGACAAGGCCCACCTCAAGGCCAACCTCATCATGCCGCAGTATCTCCGCTTGGCCCTGCGGGATTCAATTCGCTTCAAAGATCCCACCGCCAAAATCACTACGCCGGAGAACGTCCTGCCGCCGGAGGCTCCTCTGGTCGTCTTCATTAATCCGCGTAGCGGTGGACGCCATGGCCAGAAGCTCAAGCAGCGCCTCCAGCACTTAATCAGCGACGAACAGGTTTTCGATCTTTTGGATGTGAAGCCTCATGAATTTGTGCAGTACGGACTGCGTTGCCTGGAAATCTTGTCTGATCTTGGCGACGCTTGTGCCAAACAAACTCGTCAAAAGTTAAGGGTCATGGTGGCAGGAGGTGATGGCACTGTCGGTTGGGTACTGGGAAGTCTTGCAGAACTTCACAAACAAGATCGCAGCCCTGTTCTTCCAGTTGGAATTATCCCTCTGGGTACTGGAAATGACCTCTCCAGGAGTTTTGGCTGGGGAGGTTCATTTCCTTTTGCCTGGAAATACGCCGTTAAAAGATCTCTCCACAGAGCTACCTCTGGCCAAGTTCGTTCTTTGGATAGTTGGCATGTTTCGCTGTCCATGCCTGCTGGAGAAAAAGTGGAACCACCACATTCTCTGAAGCAGGTTACACAAGAAAGTGATCTGAATGAGCCTCCTGATGACAAAGGAGATTTACCAACAAAACTGGCTTGCTATGAAGGAGTGTTTTACAATTACTTGAGTATAGGAATGGATGCTCAAGTTGCTTACGGCTTCCACCATTTGCGTAACAAAAGACCTTACCTTGCTCAAGGTCCAGTGACAAATAAGCTGATTTACTCTGGTTATAGTTGCACTCAAGGTTGGTTCTTCACATCCTGCTCAAAAGATCCAAATTTGAGGGGCCTTAAGAACATCCTTAGAATACACATCAAAAAGGTCAATTGCTCGGACTGGGAAAAGATTCCAGTTCCTTCCAGTGTTAGGTCTATTGTTGCTTTAAACCTACATAACTATGCAAGTGGAAGAAACCCATGGGGTAAGCCCAAGCCAGAGTACTTGGAGAAGAGAGGGTTTGTGGAGGCACATGCTGATGATGGTCTTCTAGAGATTTTTGGGCTAAAACAAGGATGGCACACATCATTTGTTATGGTGGATATAATCTCTGCCAAACACATCGCCCAGGCAGCAGCCATTAGAATTGAAATTAGAGGTGGGAAGCGGAAGAAGGCATATCTGCAGATGGATGGCGAGCCATGGAAGCAGCAAATAAGCAAGGACTCCTCGACGTTCGTTGAAATCAAGAGGGTTCCTTTCCAATCACTAATGATCAACGGGGAC

Coding sequence (CDS)

ATGGATTCCACAGCCGACGACGTTACTATTGCTGCTCGCTCCTCCTTAATCGACTCCTTGAGGGGCTGTGGCTTGTCCGGGATCCCAATCGACAAGGCCCACCTCAAGGCCAACCTCATCATGCCGCAGTATCTCCGCTTGGCCCTGCGGGATTCAATTCGCTTCAAAGATCCCACCGCCAAAATCACTACGCCGGAGAACGTCCTGCCGCCGGAGGCTCCTCTGGTCGTCTTCATTAATCCGCGTAGCGGTGGACGCCATGGCCAGAAGCTCAAGCAGCGCCTCCAGCACTTAATCAGCGACGAACAGGTTTTCGATCTTTTGGATGTGAAGCCTCATGAATTTGTGCAGTACGGACTGCGTTGCCTGGAAATCTTGTCTGATCTTGGCGACGCTTGTGCCAAACAAACTCGTCAAAAGTTAAGGGTCATGGTGGCAGGAGGTGATGGCACTGTCGGTTGGGTACTGGGAAGTCTTGCAGAACTTCACAAACAAGATCGCAGCCCTGTTCTTCCAGTTGGAATTATCCCTCTGGGTACTGGAAATGACCTCTCCAGGAGTTTTGGCTGGGGAGGTTCATTTCCTTTTGCCTGGAAATACGCCGTTAAAAGATCTCTCCACAGAGCTACCTCTGGCCAAGTTCGTTCTTTGGATAGTTGGCATGTTTCGCTGTCCATGCCTGCTGGAGAAAAAGTGGAACCACCACATTCTCTGAAGCAGGTTACACAAGAAAGTGATCTGAATGAGCCTCCTGATGACAAAGGAGATTTACCAACAAAACTGGCTTGCTATGAAGGAGTGTTTTACAATTACTTGAGTATAGGAATGGATGCTCAAGTTGCTTACGGCTTCCACCATTTGCGTAACAAAAGACCTTACCTTGCTCAAGGTCCAGTGACAAATAAGCTGATTTACTCTGGTTATAGTTGCACTCAAGGTTGGTTCTTCACATCCTGCTCAAAAGATCCAAATTTGAGGGGCCTTAAGAACATCCTTAGAATACACATCAAAAAGGTCAATTGCTCGGACTGGGAAAAGATTCCAGTTCCTTCCAGTGTTAGGTCTATTGTTGCTTTAAACCTACATAACTATGCAAGTGGAAGAAACCCATGGGGTAAGCCCAAGCCAGAGTACTTGGAGAAGAGAGGGTTTGTGGAGGCACATGCTGATGATGGTCTTCTAGAGATTTTTGGGCTAAAACAAGGATGGCACACATCATTTGTTATGGTGGATATAATCTCTGCCAAACACATCGCCCAGGCAGCAGCCATTAGAATTGAAATTAGAGGTGGGAAGCGGAAGAAGGCATATCTGCAGATGGATGGCGAGCCATGGAAGCAGCAAATAAGCAAGGACTCCTCGACGTTCGTTGAAATCAAGAGGGTTCCTTTCCAATCACTAATGATCAACGGGGAC

Protein sequence

MDSTADDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGLRCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGTGNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQVTQESDLNEPPDDKGDLPTKLACYEGVFYNYLSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD

Homology

[Close]

BLAST of MS002280 vs. NCBI nr
Match: XP_022134720.1 (diacylglycerol kinase 7-like [Momordica charantia])

HSP 1 Score: 966.5 bits (2497), Expect = 8.6e-278
Identity = 470/474 (99.16%), Postives = 470/474 (99.16%), Query Frame = 0


Query: 1   MDSTADDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA 60
           MDSTADDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA
Sbjct: 1   MDSTADDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA 60

Query: 61  KITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 120
           KITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL
Sbjct: 61  KITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 120

Query: 121 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 180
           RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT
Sbjct: 121 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 180

Query: 181 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 240
           GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ
Sbjct: 181 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 240

Query: 241 VTQESDLNEPP--DDKGDLPTKLACYEGVFYNYLSIGMDAQVAYGFHHLRNKRPYLAQGP 300
           VTQESDLNEPP  DDKGDLPTKLACYEGVFYNY SIGMDAQVAYGFHHLRNKRPYLAQGP
Sbjct: 241 VTQESDLNEPPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP 300

Query: 301 VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA 360
           VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA
Sbjct: 301 VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA 360

Query: 361 LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 420
           LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI
Sbjct: 361 LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 420

Query: 421 AQAAAIRIEIRGGKRKKAYLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 473
           AQAAAIRIEIRGGKRKKA LQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD
Sbjct: 421 AQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 474

[Close]

BLAST of MS002280 vs. NCBI nr
Match: XP_023516209.1 (diacylglycerol kinase 7-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 849.7 bits (2194), Expect = 1.2e-242
Identity = 411/473 (86.89%), Postives = 439/473 (92.81%), Query Frame = 0


Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITT- 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLALRDSIR KDPTA  +  
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTAGASAS 68

Query: 66  --PENVLP--PEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
             P+N++P  PEAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  VPPQNIVPPQPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+KQDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGEKVEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKVEPPHSLK- 248

Query: 246 VTQESDLNEPP-DDKGDLPTKLACYEGVFYNYLSIGMDAQVAYGFHHLRNKRPYLAQGPV 305
           +T+ESDLNE   D+KGDLPTKLA YEGVFYNY SIGMDAQVAYGFHHLRNKRPYLAQGP+
Sbjct: 249 LTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPL 308

Query: 306 TNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVAL 365
           TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV L
Sbjct: 309 TNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIVTL 368

Query: 366 NLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA 425
           NLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA
Sbjct: 369 NLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA 428

Query: 426 QAAAIRIEIRGGKRKKAYLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 473
           QAAAIRIEIRGGKRKKAYLQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 QAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 480

[Close]

BLAST of MS002280 vs. NCBI nr
Match: XP_023516208.1 (diacylglycerol kinase 7-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 848.6 bits (2191), Expect = 2.6e-242
Identity = 411/475 (86.53%), Postives = 439/475 (92.42%), Query Frame = 0


Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITT- 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLALRDSIR KDPTA  +  
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTAGASAS 68

Query: 66  --PENVLP--PEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
             P+N++P  PEAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  VPPQNIVPPQPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+KQDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGEKVEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKVEPPHSLK- 248

Query: 246 VTQESDLNEPP---DDKGDLPTKLACYEGVFYNYLSIGMDAQVAYGFHHLRNKRPYLAQG 305
           +T+ESDLNE     D+KGDLPTKLA YEGVFYNY SIGMDAQVAYGFHHLRNKRPYLAQG
Sbjct: 249 LTEESDLNEAVRKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQG 308

Query: 306 PVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIV 365
           P+TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV
Sbjct: 309 PLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIV 368

Query: 366 ALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH 425
            LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH
Sbjct: 369 TLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH 428

Query: 426 IAQAAAIRIEIRGGKRKKAYLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 473
           IAQAAAIRIEIRGGKRKKAYLQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 IAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 482

[Close]

BLAST of MS002280 vs. NCBI nr
Match: KAG6589732.1 (Diacylglycerol kinase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 848.2 bits (2190), Expect = 3.4e-242
Identity = 410/473 (86.68%), Postives = 438/473 (92.60%), Query Frame = 0


Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITT- 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLALRDSIR KDPT   +  
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTTGASAS 68

Query: 66  --PENVLP--PEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
             P+N++P  PEAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  VPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+KQDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGEKVEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKVEPPHSLK- 248

Query: 246 VTQESDLNEPP-DDKGDLPTKLACYEGVFYNYLSIGMDAQVAYGFHHLRNKRPYLAQGPV 305
           +T+ESDLNE   D+KGDLPTKLA YEGVFYNY SIGMDAQVAYGFHHLRNKRPYLAQGP+
Sbjct: 249 LTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPL 308

Query: 306 TNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVAL 365
           TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV L
Sbjct: 309 TNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIVTL 368

Query: 366 NLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA 425
           NLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA
Sbjct: 369 NLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA 428

Query: 426 QAAAIRIEIRGGKRKKAYLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 473
           QAAAIRIEIRGGKRKKAYLQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 QAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 480

[Close]

BLAST of MS002280 vs. NCBI nr
Match: XP_022921758.1 (diacylglycerol kinase 7-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 846.3 bits (2185), Expect = 1.3e-241
Identity = 411/473 (86.89%), Postives = 439/473 (92.81%), Query Frame = 0


Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPT--AKIT 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLALRDSIR KDPT  A  +
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTTGASAS 68

Query: 66  TP-ENVLP--PEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
            P +N++P  PEAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  VPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+KQDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGEKVEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKVEPPHSLK- 248

Query: 246 VTQESDLNEPP-DDKGDLPTKLACYEGVFYNYLSIGMDAQVAYGFHHLRNKRPYLAQGPV 305
           +T+ESDLNE   D+KGDLPTKLA YEGVFYNY SIGMDAQVAYGFHHLRNKRPYLAQGP+
Sbjct: 249 LTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPL 308

Query: 306 TNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVAL 365
           TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV L
Sbjct: 309 TNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIVTL 368

Query: 366 NLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA 425
           NLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA
Sbjct: 369 NLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA 428

Query: 426 QAAAIRIEIRGGKRKKAYLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 473
           QAAAIRIEIRGGKRKKAYLQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 QAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 480

[Close]

BLAST of MS002280 vs. ExPASy Swiss-Prot
Match: F4JQ95 (Diacylglycerol kinase 7 OS=Arabidopsis thaliana OX=3702 GN=DGK7 PE=1 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 2.0e-189
Identity = 310/463 (66.95%), Postives = 373/463 (80.56%), Query Frame = 0


Query: 19  SLRGCG---LSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA---KITTPENV---L 78
           ++RGCG   L+ + IDK  L+  L MP+YLR+A+RD I+ KD T     +  P      +
Sbjct: 30  TMRGCGFANLALVGIDKEELRGRLAMPEYLRIAMRDCIKRKDSTEISDHLLLPGGAAADM 89

Query: 79  PPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGLRCLEILSDL 138
            P AP+VVFINP+SGGRHG  LK+RLQ L+++EQVFDL +VKPHEFV+YGL CL+ L+  
Sbjct: 90  APHAPMVVFINPKSGGRHGPVLKERLQQLMTEEQVFDLTEVKPHEFVRYGLGCLDTLAAK 149

Query: 139 GDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGTGNDLSRSFG 198
           GD CA++ R+K+R+MVAGGDGTVGWVLG L ELHK  +S + PVG+IPLGTGNDLSRSF 
Sbjct: 150 GDECARECREKIRIMVAGGDGTVGWVLGCLGELHKDGKSHIPPVGVIPLGTGNDLSRSFS 209

Query: 199 WGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQVTQESDLNE 258
           WGGSFPFAW+ A+KR+LHRAT G +  LDSW + +SMP+GE V+PP+SLK   +E+ L++
Sbjct: 210 WGGSFPFAWRSAMKRTLHRATLGSIARLDSWKIVVSMPSGEVVDPPYSLKPTIEETALDQ 269

Query: 259 PPDDKGDLPTKLACYEGVFYNYLSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSGYS 318
             D  GD+P K   YEGVFYNY SIGMDAQVAYGFHHLRN++PYLAQGPVTNK+IYS YS
Sbjct: 270 ALDADGDVPPKAKSYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPVTNKIIYSSYS 329

Query: 319 CTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVALNLHNYASGRN 378
           CTQGWF T C  +P LRGL+NI++IHIKK NCS+WE+I VP SVRSIV LNL+NY SGR+
Sbjct: 330 CTQGWFCTPCVNNPALRGLRNIMKIHIKKANCSEWEEIHVPKSVRSIVVLNLYNYGSGRH 389

Query: 379 PWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIR 438
           PWG  +P+YLEKRGFVEAH DDGL+EIFGLKQGWH SFVM +IISAKHIAQAAAIR E+R
Sbjct: 390 PWGNLRPKYLEKRGFVEAHCDDGLIEIFGLKQGWHASFVMAEIISAKHIAQAAAIRFELR 449

Query: 439 GGKRKKAYLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 473
           GG  K A+LQMDGEPWKQ +  D STFVEIK+VPFQSLMING+
Sbjct: 450 GGDWKNAFLQMDGEPWKQPMKSDYSTFVEIKKVPFQSLMINGE 492

[Close]

BLAST of MS002280 vs. ExPASy Swiss-Prot
Match: Q8VZG1 (Diacylglycerol kinase 3 OS=Arabidopsis thaliana OX=3702 GN=DGK3 PE=1 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 5.2e-185
Identity = 309/477 (64.78%), Postives = 378/477 (79.25%), Query Frame = 0


Query: 10  IAARSSLIDS--LRGCGLSG---IPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITT 69
           +A+R S  DS  +RGCGL+    + +DK  L+  L+MP+YLRLA+RD I+ KD +A    
Sbjct: 16  VASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMMPEYLRLAMRDCIKRKDSSA---I 75

Query: 70  PENVL---------PPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEF 129
           P+++L          P AP+VVFINP SGGRHG  LK+RLQ L+S+EQVFDL +VKPHEF
Sbjct: 76  PDHLLLPGGAVADMAPHAPMVVFINPNSGGRHGPVLKERLQQLMSEEQVFDLTEVKPHEF 135

Query: 130 VQYGLRCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGI 189
           V+YGL CLE ++  GD CAK+ R +LR+MVAGGDGTVGWVLG L EL+K  +S + PVG+
Sbjct: 136 VRYGLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVGWVLGCLGELNKDGKSQIPPVGV 195

Query: 190 IPLGTGNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPP 249
           IPLGTGNDLSRSFGWGGSFPFAW+ AVKR+LHRA+ G V  LDSW + +SMP+GE V+PP
Sbjct: 196 IPLGTGNDLSRSFGWGGSFPFAWRSAVKRTLHRASMGPVARLDSWKILVSMPSGEVVDPP 255

Query: 250 HSLKQVTQESDLNEPPDDKGDLPTKLACYEGVFYNYLSIGMDAQVAYGFHHLRNKRPYLA 309
           +SLK   +E++L++  D   D P     YEGVFYNYLSIGMDAQVAYGFHHLRN +PYLA
Sbjct: 256 YSLKP-AEENELDQGLDAGIDAPPLAKAYEGVFYNYLSIGMDAQVAYGFHHLRNTKPYLA 315

Query: 310 QGPVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRS 369
           QGP++NK+IYS + C+QGWF T C  DP LRGL+NI++IHIKKVNCS WE+I VP +VRS
Sbjct: 316 QGPISNKIIYSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHIKKVNCSQWEEIAVPKNVRS 375

Query: 370 IVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISA 429
           IVALNLH+Y SG +PWG  KP+YLEKRGFVEAH DDGL+EIFG KQGWH SFVM ++ISA
Sbjct: 376 IVALNLHSYGSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEIFGFKQGWHASFVMAELISA 435

Query: 430 KHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 473
           KHIAQAAA+R E+RGG  + A+LQMDGEPWKQ +S + STFVEIK+VP+QSLMIN +
Sbjct: 436 KHIAQAAAVRFELRGGDWRDAFLQMDGEPWKQPMSTEYSTFVEIKKVPYQSLMINNE 488

[Close]

BLAST of MS002280 vs. ExPASy Swiss-Prot
Match: Q1PDI2 (Diacylglycerol kinase 4 OS=Arabidopsis thaliana OX=3702 GN=DGK4 PE=1 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 2.0e-181
Identity = 293/454 (64.54%), Postives = 369/454 (81.28%), Query Frame = 0


Query: 25  LSGIPIDKAHLKANLIMPQYLRLALRDSIRFK----DPTAKITTP--ENVLPPEAPLVVF 84
           L+ I + KA L+  +++PQYLR+A+RD I  K    D ++ +  P   N L PE PL+VF
Sbjct: 35  LASIRVSKAELRQRVMLPQYLRIAIRDCILRKDDSFDASSSVAPPLENNALTPEVPLMVF 94

Query: 85  INPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGLRCLEILSDLGDACAKQTR 144
           +NP+SGGR G  +K+RLQ+LIS+EQV+DL +VKP+EF++YGL CLE  +  GD CAK+ R
Sbjct: 95  VNPKSGGRQGPLIKERLQNLISEEQVYDLTEVKPNEFIRYGLGCLEAFASRGDECAKEIR 154

Query: 145 QKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGTGNDLSRSFGWGGSFPFAW 204
           +K+R++VAGGDGTVGWVLG L EL+ Q+R PV PV I+PLGTGNDLSRSFGWGGSFPFAW
Sbjct: 155 EKMRIVVAGGDGTVGWVLGCLGELNLQNRLPVPPVSIMPLGTGNDLSRSFGWGGSFPFAW 214

Query: 205 KYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQVTQESDLNEPPDDKGDLP 264
           K A+KR+LHRA+   +  LDSW++ ++MP+GE V+PP+SLK  TQE  +++  + +G++P
Sbjct: 215 KSAIKRTLHRASVAPISRLDSWNILITMPSGEIVDPPYSLK-ATQECYIDQNLEIEGEIP 274

Query: 265 TKLACYEGVFYNYLSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSGYSCTQGWFFTS 324
                YEGVFYNY SIGMDAQVAYGFHHLRN++PYLA GP+ NK+IYSGY C+QGWF T 
Sbjct: 275 PSTNGYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLANGPIANKIIYSGYGCSQGWFLTH 334

Query: 325 CSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVALNLHNYASGRNPWGKPKPEY 384
           C  DP LRGLKNI+ +HIKK++ S+WEK+PVP SVR++VALNLH+Y SGRNPWG  K +Y
Sbjct: 335 CINDPGLRGLKNIMTLHIKKLDSSEWEKVPVPKSVRAVVALNLHSYGSGRNPWGNLKQDY 394

Query: 385 LEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYL 444
           LEKRGFVEA ADDGLLEIFGLKQGWH SFVMV++ISAKHIAQAAAIR+EIRGG  K A++
Sbjct: 395 LEKRGFVEAQADDGLLEIFGLKQGWHASFVMVELISAKHIAQAAAIRLEIRGGDWKDAFM 454

Query: 445 QMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 473
           QMDGEPWKQ +++D STFV+IKRVP QSL++ GD
Sbjct: 455 QMDGEPWKQPMTRDYSTFVDIKRVPHQSLVVKGD 487

[Close]

BLAST of MS002280 vs. ExPASy Swiss-Prot
Match: F4JKI3 (Diacylglycerol kinase 6 OS=Arabidopsis thaliana OX=3702 GN=DGK6 PE=3 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 5.0e-71
Identity = 149/410 (36.34%), Postives = 228/410 (55.61%), Query Frame = 0


Query: 57  DPTAKITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFV 116
           +P A+I+       PE P++VFIN +SGG+ G +L    + L++D+QVFDL    P + +
Sbjct: 33  EPEAQISC---TTAPENPILVFINSKSGGQLGAELILTYRTLLNDKQVFDLEVETPDKVL 92

Query: 117 QYGLRCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGII 176
           Q     LE L D  D+ A + R KL+++VAGGDGT GW+LG +++L+  +     P+  +
Sbjct: 93  QRIYLNLERLKD--DSLASKIRDKLKIIVAGGDGTAGWLLGVVSDLNLSNPP---PIATV 152

Query: 177 PLGTGNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEK----- 236
           PLGTGN+L  +FGWG   P   + +V+  L +  + +   +D+W + + M   ++     
Sbjct: 153 PLGTGNNLPFAFGWGKKNPGTDRSSVESFLGKVINAKEMKIDNWKILMRMKHPKEGSCDI 212

Query: 237 -VEPPHSLKQVTQESDLNEPPDDKGDLPTKLACYEGVFYNYLSIGMDAQVAYGFHHLRNK 296
            ++ PHSL ++         P D+ ++      Y G F+NY S+GMDAQV+Y FH  R  
Sbjct: 213 TLKLPHSLPRIF--------PSDQENM-EGYHTYRGGFWNYFSLGMDAQVSYAFHSQRKL 272

Query: 297 RPYLAQGPVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVP 356
            P   +  + N+  Y   SCTQGWFF S    P+ + +  + +I I   N   W  + +P
Sbjct: 273 HPERFKNQLVNQSTYLKLSCTQGWFFASLF-HPSSQNIAKLAKIQICDRN-GQWNDLHIP 332

Query: 357 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 416
            S+RSIV LNL +++ G NPWG P P+    R       DDGL+EI G +  WH   ++ 
Sbjct: 333 QSIRSIVCLNLPSFSGGLNPWGTPNPKKQRDRSLTAPFVDDGLIEIVGFRNAWHGLILLS 392

Query: 417 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQQI-SKDSSTFVEI 460
                  +AQA  +R+E + G  K AY+++DGEPWKQ + S D +  VEI
Sbjct: 393 PNGHGTRLAQANRVRLEFKKGAAKHAYMRIDGEPWKQPLPSNDETVMVEI 423

[Close]

BLAST of MS002280 vs. ExPASy Swiss-Prot
Match: Q7XQT2 (Diacylglycerol kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DGK1 PE=1 SV=2)

HSP 1 Score: 268.9 bits (686), Expect = 1.1e-70
Identity = 153/424 (36.08%), Postives = 223/424 (52.59%), Query Frame = 0


Query: 57  DPTAKITTPENVLPPEA-----------PLVVFINPRSGGRHGQKLKQRLQHLISDEQVF 116
           +P      P+ +L P++           P+VVFIN RSGG+ G  L +  + L++  QVF
Sbjct: 17  EPLTDYCIPDYILNPDSEQVLVDQAPCCPVVVFINSRSGGQLGSSLIKTYRELLNKAQVF 76

Query: 117 DLLDVKPHEFVQYGLRC-LEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHK 176
           DL +  P E V + L C  E L   GD  A Q +  LR++VAGGDGT  W+LG +++L K
Sbjct: 77  DLSEEAP-EKVLHRLYCNFEKLKSNGDPIAFQIQSNLRLIVAGGDGTASWLLGVVSDL-K 136

Query: 177 QDRSPVLPVGIIPLGTGNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSL 236
               P  P+  +PLGTGN+L  SFGWG   P   + AVK  L +    +  ++DSWH+ +
Sbjct: 137 LSHPP--PIATVPLGTGNNLPFSFGWGKKNPTTDQEAVKSFLGQVKKAREMNIDSWHIIM 196

Query: 237 SMPAGEK--------VEPPHSL---KQVTQESDLNEPPDDKGDLPTKLACYEGVFYNYLS 296
            M A ++        +E PHSL    +V+    LN               Y G F+NY S
Sbjct: 197 RMRAPQEGPCEPIAPLELPHSLHAFHRVSGSDSLN---------MEGYHTYRGGFWNYFS 256

Query: 297 IGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILR 356
           +GMDAQV+Y FH  R + P   +  +TN+  Y+     QGWF  S +  P+ R +  + +
Sbjct: 257 MGMDAQVSYEFHSERKRNPEKFKNQLTNQSTYAKLGLKQGWFAASLT-HPSSRNIAQLAK 316

Query: 357 IHIKKVNCSDWEKIPVPSSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGL 416
           + I K     WE++ +P S+RSIV LNL +++ G NPWG P    ++ R       DDGL
Sbjct: 317 VRIMKRPGGQWEELKIPRSIRSIVCLNLPSFSGGLNPWGTPGTRKVQDRDLTAPFVDDGL 376

Query: 417 LEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQQISKDS 458
           +E+ G +  WH   ++        +AQA  IR E   G  +  ++++DGEPWKQ + KD 
Sbjct: 377 IEVVGFRDAWHGLVLLAPNGHGTRLAQAHRIRFEFHKGAAEHTFMRIDGEPWKQPLPKDD 426

[Close]

BLAST of MS002280 vs. ExPASy TrEMBL
Match: A0A6J1BZ41 (Diacylglycerol kinase OS=Momordica charantia OX=3673 GN=LOC111006923 PE=3 SV=1)

HSP 1 Score: 966.5 bits (2497), Expect = 4.2e-278
Identity = 470/474 (99.16%), Postives = 470/474 (99.16%), Query Frame = 0


Query: 1   MDSTADDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA 60
           MDSTADDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA
Sbjct: 1   MDSTADDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA 60

Query: 61  KITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 120
           KITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL
Sbjct: 61  KITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 120

Query: 121 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 180
           RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT
Sbjct: 121 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 180

Query: 181 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 240
           GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ
Sbjct: 181 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 240

Query: 241 VTQESDLNEPP--DDKGDLPTKLACYEGVFYNYLSIGMDAQVAYGFHHLRNKRPYLAQGP 300
           VTQESDLNEPP  DDKGDLPTKLACYEGVFYNY SIGMDAQVAYGFHHLRNKRPYLAQGP
Sbjct: 241 VTQESDLNEPPVHDDKGDLPTKLACYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGP 300

Query: 301 VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA 360
           VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA
Sbjct: 301 VTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVA 360

Query: 361 LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 420
           LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI
Sbjct: 361 LNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHI 420

Query: 421 AQAAAIRIEIRGGKRKKAYLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 473
           AQAAAIRIEIRGGKRKKA LQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD
Sbjct: 421 AQAAAIRIEIRGGKRKKACLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 474

[Close]

BLAST of MS002280 vs. ExPASy TrEMBL
Match: A0A6J1E4R5 (diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111429914 PE=3 SV=1)

HSP 1 Score: 846.3 bits (2185), Expect = 6.3e-242
Identity = 411/473 (86.89%), Postives = 439/473 (92.81%), Query Frame = 0


Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPT--AKIT 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLALRDSIR KDPT  A  +
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTTGASAS 68

Query: 66  TP-ENVLP--PEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
            P +N++P  PEAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  VPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+KQDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGEKVEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKVEPPHSLK- 248

Query: 246 VTQESDLNEPP-DDKGDLPTKLACYEGVFYNYLSIGMDAQVAYGFHHLRNKRPYLAQGPV 305
           +T+ESDLNE   D+KGDLPTKLA YEGVFYNY SIGMDAQVAYGFHHLRNKRPYLAQGP+
Sbjct: 249 LTEESDLNEAKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQGPL 308

Query: 306 TNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVAL 365
           TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV L
Sbjct: 309 TNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIVTL 368

Query: 366 NLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA 425
           NLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA
Sbjct: 369 NLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA 428

Query: 426 QAAAIRIEIRGGKRKKAYLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 473
           QAAAIRIEIRGGKRKKAYLQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 QAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 480

[Close]

BLAST of MS002280 vs. ExPASy TrEMBL
Match: A0A6J1E1C6 (diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111429914 PE=3 SV=1)

HSP 1 Score: 845.1 bits (2182), Expect = 1.4e-241
Identity = 411/475 (86.53%), Postives = 439/475 (92.42%), Query Frame = 0


Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPT--AKIT 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLALRDSIR KDPT  A  +
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLALRDSIRLKDPTTGASAS 68

Query: 66  TP-ENVLP--PEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
            P +N++P  PEAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  VPLQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+KQDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELYKQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGEKVEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGEKVEPPHSLK- 248

Query: 246 VTQESDLNEPP---DDKGDLPTKLACYEGVFYNYLSIGMDAQVAYGFHHLRNKRPYLAQG 305
           +T+ESDLNE     D+KGDLPTKLA YEGVFYNY SIGMDAQVAYGFHHLRNKRPYLAQG
Sbjct: 249 LTEESDLNEAVRKLDEKGDLPTKLASYEGVFYNYFSIGMDAQVAYGFHHLRNKRPYLAQG 308

Query: 306 PVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIV 365
           P+TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV
Sbjct: 309 PLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIV 368

Query: 366 ALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH 425
            LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH
Sbjct: 369 TLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH 428

Query: 426 IAQAAAIRIEIRGGKRKKAYLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 473
           IAQAAAIRIEIRGGKRKKAYLQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 IAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 482

[Close]

BLAST of MS002280 vs. ExPASy TrEMBL
Match: A0A6J1JHE1 (diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111485730 PE=3 SV=1)

HSP 1 Score: 837.8 bits (2163), Expect = 2.2e-239
Identity = 405/473 (85.62%), Postives = 436/473 (92.18%), Query Frame = 0


Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITT- 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLA+RDSIR KDPTA  +  
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLAMRDSIRLKDPTAGASAS 68

Query: 66  --PENVLP--PEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
             P+N++P  PEAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  LPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+ QDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELNNQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGE+VEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGERVEPPHSLK- 248

Query: 246 VTQESDLNEPP-DDKGDLPTKLACYEGVFYNYLSIGMDAQVAYGFHHLRNKRPYLAQGPV 305
           +T+ESDLNE   D+KGDLPTKLA Y GVFYNY SIGMDAQVAYGFHHLRNKRP LA+GP+
Sbjct: 249 LTEESDLNEAKLDEKGDLPTKLASYGGVFYNYFSIGMDAQVAYGFHHLRNKRPKLARGPL 308

Query: 306 TNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVAL 365
           TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV L
Sbjct: 309 TNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIVTL 368

Query: 366 NLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA 425
           NLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA
Sbjct: 369 NLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA 428

Query: 426 QAAAIRIEIRGGKRKKAYLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 473
           QAAAIRIEIRGGKRKKAYLQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 QAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 480

[Close]

BLAST of MS002280 vs. ExPASy TrEMBL
Match: A0A6J1JJR6 (diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111485730 PE=3 SV=1)

HSP 1 Score: 836.6 bits (2160), Expect = 5.0e-239
Identity = 405/475 (85.26%), Postives = 436/475 (91.79%), Query Frame = 0


Query: 6   DDVTIAARSSLIDSLRGCGLSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITT- 65
           D V+IAARSSL DSL+GCGLSGIPIDK  L   ++MPQYLRLA+RDSIR KDPTA  +  
Sbjct: 9   DQVSIAARSSLFDSLKGCGLSGIPIDKQELNTKVMMPQYLRLAMRDSIRLKDPTAGASAS 68

Query: 66  --PENVLP--PEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGL 125
             P+N++P  PEAPLVVFINPRSGGRHGQKLKQRLQ LIS+EQVFDLL+VKPHEFVQYGL
Sbjct: 69  LPPQNIVPPLPEAPLVVFINPRSGGRHGQKLKQRLQQLISEEQVFDLLEVKPHEFVQYGL 128

Query: 126 RCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGT 185
           RCLEIL+D GD CAKQTRQ++RVMVAGGDGTVGWVLGSLAEL+ QDR PVLPVGIIPLGT
Sbjct: 129 RCLEILADQGDICAKQTRQRMRVMVAGGDGTVGWVLGSLAELNNQDRKPVLPVGIIPLGT 188

Query: 186 GNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQ 245
           GNDLSRSFGWGGSFPFAW+ AVKRSLHRAT+GQV SLDSWHV LS+PAGE+VEPPHSLK 
Sbjct: 189 GNDLSRSFGWGGSFPFAWQSAVKRSLHRATTGQVGSLDSWHVLLSVPAGERVEPPHSLK- 248

Query: 246 VTQESDLNEPP---DDKGDLPTKLACYEGVFYNYLSIGMDAQVAYGFHHLRNKRPYLAQG 305
           +T+ESDLNE     D+KGDLPTKLA Y GVFYNY SIGMDAQVAYGFHHLRNKRP LA+G
Sbjct: 249 LTEESDLNEAVRKLDEKGDLPTKLASYGGVFYNYFSIGMDAQVAYGFHHLRNKRPKLARG 308

Query: 306 PVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIV 365
           P+TNKLIYS YSCTQGWFFTSCS DPNLRGLKNI+RIHIKK+NCS+WEKIP+PSSVRSIV
Sbjct: 309 PLTNKLIYSSYSCTQGWFFTSCSNDPNLRGLKNIVRIHIKKINCSEWEKIPIPSSVRSIV 368

Query: 366 ALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH 425
            LNLHNYASGRNPWGKPKP+YLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH
Sbjct: 369 TLNLHNYASGRNPWGKPKPQYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKH 428

Query: 426 IAQAAAIRIEIRGGKRKKAYLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 473
           IAQAAAIRIEIRGGKRKKAYLQMDGEPWKQ I+KD ST+VEIKRVPFQSLMINGD
Sbjct: 429 IAQAAAIRIEIRGGKRKKAYLQMDGEPWKQPINKDFSTYVEIKRVPFQSLMINGD 482

[Close]

BLAST of MS002280 vs. TAIR 10
Match: AT4G30340.1 (diacylglycerol kinase 7 )

HSP 1 Score: 663.3 bits (1710), Expect = 1.5e-190
Identity = 310/463 (66.95%), Postives = 373/463 (80.56%), Query Frame = 0


Query: 19  SLRGCG---LSGIPIDKAHLKANLIMPQYLRLALRDSIRFKDPTA---KITTPENV---L 78
           ++RGCG   L+ + IDK  L+  L MP+YLR+A+RD I+ KD T     +  P      +
Sbjct: 30  TMRGCGFANLALVGIDKEELRGRLAMPEYLRIAMRDCIKRKDSTEISDHLLLPGGAAADM 89

Query: 79  PPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGLRCLEILSDL 138
            P AP+VVFINP+SGGRHG  LK+RLQ L+++EQVFDL +VKPHEFV+YGL CL+ L+  
Sbjct: 90  APHAPMVVFINPKSGGRHGPVLKERLQQLMTEEQVFDLTEVKPHEFVRYGLGCLDTLAAK 149

Query: 139 GDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGTGNDLSRSFG 198
           GD CA++ R+K+R+MVAGGDGTVGWVLG L ELHK  +S + PVG+IPLGTGNDLSRSF 
Sbjct: 150 GDECARECREKIRIMVAGGDGTVGWVLGCLGELHKDGKSHIPPVGVIPLGTGNDLSRSFS 209

Query: 199 WGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQVTQESDLNE 258
           WGGSFPFAW+ A+KR+LHRAT G +  LDSW + +SMP+GE V+PP+SLK   +E+ L++
Sbjct: 210 WGGSFPFAWRSAMKRTLHRATLGSIARLDSWKIVVSMPSGEVVDPPYSLKPTIEETALDQ 269

Query: 259 PPDDKGDLPTKLACYEGVFYNYLSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSGYS 318
             D  GD+P K   YEGVFYNY SIGMDAQVAYGFHHLRN++PYLAQGPVTNK+IYS YS
Sbjct: 270 ALDADGDVPPKAKSYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPVTNKIIYSSYS 329

Query: 319 CTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVALNLHNYASGRN 378
           CTQGWF T C  +P LRGL+NI++IHIKK NCS+WE+I VP SVRSIV LNL+NY SGR+
Sbjct: 330 CTQGWFCTPCVNNPALRGLRNIMKIHIKKANCSEWEEIHVPKSVRSIVVLNLYNYGSGRH 389

Query: 379 PWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIR 438
           PWG  +P+YLEKRGFVEAH DDGL+EIFGLKQGWH SFVM +IISAKHIAQAAAIR E+R
Sbjct: 390 PWGNLRPKYLEKRGFVEAHCDDGLIEIFGLKQGWHASFVMAEIISAKHIAQAAAIRFELR 449

Query: 439 GGKRKKAYLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 473
           GG  K A+LQMDGEPWKQ +  D STFVEIK+VPFQSLMING+
Sbjct: 450 GGDWKNAFLQMDGEPWKQPMKSDYSTFVEIKKVPFQSLMINGE 492

[Close]

BLAST of MS002280 vs. TAIR 10
Match: AT2G18730.1 (diacylglycerol kinase 3 )

HSP 1 Score: 648.7 bits (1672), Expect = 3.7e-186
Identity = 309/477 (64.78%), Postives = 378/477 (79.25%), Query Frame = 0


Query: 10  IAARSSLIDS--LRGCGLSG---IPIDKAHLKANLIMPQYLRLALRDSIRFKDPTAKITT 69
           +A+R S  DS  +RGCGL+    + +DK  L+  L+MP+YLRLA+RD I+ KD +A    
Sbjct: 16  VASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMMPEYLRLAMRDCIKRKDSSA---I 75

Query: 70  PENVL---------PPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEF 129
           P+++L          P AP+VVFINP SGGRHG  LK+RLQ L+S+EQVFDL +VKPHEF
Sbjct: 76  PDHLLLPGGAVADMAPHAPMVVFINPNSGGRHGPVLKERLQQLMSEEQVFDLTEVKPHEF 135

Query: 130 VQYGLRCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGI 189
           V+YGL CLE ++  GD CAK+ R +LR+MVAGGDGTVGWVLG L EL+K  +S + PVG+
Sbjct: 136 VRYGLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVGWVLGCLGELNKDGKSQIPPVGV 195

Query: 190 IPLGTGNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPP 249
           IPLGTGNDLSRSFGWGGSFPFAW+ AVKR+LHRA+ G V  LDSW + +SMP+GE V+PP
Sbjct: 196 IPLGTGNDLSRSFGWGGSFPFAWRSAVKRTLHRASMGPVARLDSWKILVSMPSGEVVDPP 255

Query: 250 HSLKQVTQESDLNEPPDDKGDLPTKLACYEGVFYNYLSIGMDAQVAYGFHHLRNKRPYLA 309
           +SLK   +E++L++  D   D P     YEGVFYNYLSIGMDAQVAYGFHHLRN +PYLA
Sbjct: 256 YSLKP-AEENELDQGLDAGIDAPPLAKAYEGVFYNYLSIGMDAQVAYGFHHLRNTKPYLA 315

Query: 310 QGPVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRS 369
           QGP++NK+IYS + C+QGWF T C  DP LRGL+NI++IHIKKVNCS WE+I VP +VRS
Sbjct: 316 QGPISNKIIYSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHIKKVNCSQWEEIAVPKNVRS 375

Query: 370 IVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISA 429
           IVALNLH+Y SG +PWG  KP+YLEKRGFVEAH DDGL+EIFG KQGWH SFVM ++ISA
Sbjct: 376 IVALNLHSYGSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEIFGFKQGWHASFVMAELISA 435

Query: 430 KHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 473
           KHIAQAAA+R E+RGG  + A+LQMDGEPWKQ +S + STFVEIK+VP+QSLMIN +
Sbjct: 436 KHIAQAAAVRFELRGGDWRDAFLQMDGEPWKQPMSTEYSTFVEIKKVPYQSLMINNE 488

[Close]

BLAST of MS002280 vs. TAIR 10
Match: AT5G57690.1 (diacylglycerol kinase 4 )

HSP 1 Score: 636.7 bits (1641), Expect = 1.5e-182
Identity = 293/454 (64.54%), Postives = 369/454 (81.28%), Query Frame = 0


Query: 25  LSGIPIDKAHLKANLIMPQYLRLALRDSIRFK----DPTAKITTP--ENVLPPEAPLVVF 84
           L+ I + KA L+  +++PQYLR+A+RD I  K    D ++ +  P   N L PE PL+VF
Sbjct: 35  LASIRVSKAELRQRVMLPQYLRIAIRDCILRKDDSFDASSSVAPPLENNALTPEVPLMVF 94

Query: 85  INPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGLRCLEILSDLGDACAKQTR 144
           +NP+SGGR G  +K+RLQ+LIS+EQV+DL +VKP+EF++YGL CLE  +  GD CAK+ R
Sbjct: 95  VNPKSGGRQGPLIKERLQNLISEEQVYDLTEVKPNEFIRYGLGCLEAFASRGDECAKEIR 154

Query: 145 QKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGTGNDLSRSFGWGGSFPFAW 204
           +K+R++VAGGDGTVGWVLG L EL+ Q+R PV PV I+PLGTGNDLSRSFGWGGSFPFAW
Sbjct: 155 EKMRIVVAGGDGTVGWVLGCLGELNLQNRLPVPPVSIMPLGTGNDLSRSFGWGGSFPFAW 214

Query: 205 KYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEKVEPPHSLKQVTQESDLNEPPDDKGDLP 264
           K A+KR+LHRA+   +  LDSW++ ++MP+GE V+PP+SLK  TQE  +++  + +G++P
Sbjct: 215 KSAIKRTLHRASVAPISRLDSWNILITMPSGEIVDPPYSLK-ATQECYIDQNLEIEGEIP 274

Query: 265 TKLACYEGVFYNYLSIGMDAQVAYGFHHLRNKRPYLAQGPVTNKLIYSGYSCTQGWFFTS 324
                YEGVFYNY SIGMDAQVAYGFHHLRN++PYLA GP+ NK+IYSGY C+QGWF T 
Sbjct: 275 PSTNGYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLANGPIANKIIYSGYGCSQGWFLTH 334

Query: 325 CSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVALNLHNYASGRNPWGKPKPEY 384
           C  DP LRGLKNI+ +HIKK++ S+WEK+PVP SVR++VALNLH+Y SGRNPWG  K +Y
Sbjct: 335 CINDPGLRGLKNIMTLHIKKLDSSEWEKVPVPKSVRAVVALNLHSYGSGRNPWGNLKQDY 394

Query: 385 LEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIAQAAAIRIEIRGGKRKKAYL 444
           LEKRGFVEA ADDGLLEIFGLKQGWH SFVMV++ISAKHIAQAAAIR+EIRGG  K A++
Sbjct: 395 LEKRGFVEAQADDGLLEIFGLKQGWHASFVMVELISAKHIAQAAAIRLEIRGGDWKDAFM 454

Query: 445 QMDGEPWKQQISKDSSTFVEIKRVPFQSLMINGD 473
           QMDGEPWKQ +++D STFV+IKRVP QSL++ GD
Sbjct: 455 QMDGEPWKQPMTRDYSTFVDIKRVPHQSLVVKGD 487

[Close]

BLAST of MS002280 vs. TAIR 10
Match: AT4G28130.1 (diacylglycerol kinase 6 )

HSP 1 Score: 270.0 bits (689), Expect = 3.6e-72
Identity = 149/410 (36.34%), Postives = 228/410 (55.61%), Query Frame = 0


Query: 57  DPTAKITTPENVLPPEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFV 116
           +P A+I+       PE P++VFIN +SGG+ G +L    + L++D+QVFDL    P + +
Sbjct: 33  EPEAQISC---TTAPENPILVFINSKSGGQLGAELILTYRTLLNDKQVFDLEVETPDKVL 92

Query: 117 QYGLRCLEILSDLGDACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGII 176
           Q     LE L D  D+ A + R KL+++VAGGDGT GW+LG +++L+  +     P+  +
Sbjct: 93  QRIYLNLERLKD--DSLASKIRDKLKIIVAGGDGTAGWLLGVVSDLNLSNPP---PIATV 152

Query: 177 PLGTGNDLSRSFGWGGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEK----- 236
           PLGTGN+L  +FGWG   P   + +V+  L +  + +   +D+W + + M   ++     
Sbjct: 153 PLGTGNNLPFAFGWGKKNPGTDRSSVESFLGKVINAKEMKIDNWKILMRMKHPKEGSCDI 212

Query: 237 -VEPPHSLKQVTQESDLNEPPDDKGDLPTKLACYEGVFYNYLSIGMDAQVAYGFHHLRNK 296
            ++ PHSL ++         P D+ ++      Y G F+NY S+GMDAQV+Y FH  R  
Sbjct: 213 TLKLPHSLPRIF--------PSDQENM-EGYHTYRGGFWNYFSLGMDAQVSYAFHSQRKL 272

Query: 297 RPYLAQGPVTNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVP 356
            P   +  + N+  Y   SCTQGWFF S    P+ + +  + +I I   N   W  + +P
Sbjct: 273 HPERFKNQLVNQSTYLKLSCTQGWFFASLF-HPSSQNIAKLAKIQICDRN-GQWNDLHIP 332

Query: 357 SSVRSIVALNLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMV 416
            S+RSIV LNL +++ G NPWG P P+    R       DDGL+EI G +  WH   ++ 
Sbjct: 333 QSIRSIVCLNLPSFSGGLNPWGTPNPKKQRDRSLTAPFVDDGLIEIVGFRNAWHGLILLS 392

Query: 417 DIISAKHIAQAAAIRIEIRGGKRKKAYLQMDGEPWKQQI-SKDSSTFVEI 460
                  +AQA  +R+E + G  K AY+++DGEPWKQ + S D +  VEI
Sbjct: 393 PNGHGTRLAQANRVRLEFKKGAAKHAYMRIDGEPWKQPLPSNDETVMVEI 423

[Close]

BLAST of MS002280 vs. TAIR 10
Match: AT2G20900.1 (diacylglycerol kinase 5 )

HSP 1 Score: 264.2 bits (674), Expect = 2.0e-70
Identity = 148/414 (35.75%), Postives = 218/414 (52.66%), Query Frame = 0


Query: 71  PEAPLVVFINPRSGGRHGQKLKQRLQHLISDEQVFDLLDVKPHEFVQYGLRCLEILSDLG 130
           P +P++VFIN +SGG+ G +L    + L++  QVFDL    P + ++     LE L D  
Sbjct: 37  PASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNLERLKD-- 96

Query: 131 DACAKQTRQKLRVMVAGGDGTVGWVLGSLAELHKQDRSPVLPVGIIPLGTGNDLSRSFGW 190
           D  A+Q R+KL+++VAGGDGT GW+LG + +L K    P  P+  +PLGTGN+L  +FGW
Sbjct: 97  DDFARQIREKLKIIVAGGDGTAGWLLGVVCDL-KLSHPP--PIATVPLGTGNNLPFAFGW 156

Query: 191 GGSFPFAWKYAVKRSLHRATSGQVRSLDSWHVSLSMPAGEK---------VEPPHSLKQV 250
           G   P   + AV+  L +    +V  +D+WH+ + M   ++         +E PHSL   
Sbjct: 157 GKKNPGTDRTAVESFLEQVLKAKVMKIDNWHILMRMKTPKEGGSCDPVAPLELPHSLHAF 216

Query: 251 TQESDLNEPPDDKGDLPTKLAC--YEGVFYNYLSIGMDAQVAYGFHHLRNKRPYLAQGPV 310
            + S  +E          K  C  + G F+NY S+GMDAQ++Y FH  R   P   +  +
Sbjct: 217 HRVSPTDE--------LNKEGCHTFRGGFWNYFSLGMDAQISYAFHSERKLHPEKFKNQL 276

Query: 311 TNKLIYSGYSCTQGWFFTSCSKDPNLRGLKNILRIHIKKVNCSDWEKIPVPSSVRSIVAL 370
            N+  Y    CTQGWF  S    P  R +  + ++ I   N   W+ + +P S+RSIV L
Sbjct: 277 VNQSTYVKLGCTQGWFCASLF-HPASRNIAQLAKVKIATRN-GQWQDLHIPHSIRSIVCL 336

Query: 371 NLHNYASGRNPWGKPKPEYLEKRGFVEAHADDGLLEIFGLKQGWHTSFVMVDIISAKHIA 430
           NL +++ G NPWG P P     RG      DDGL+E+ G +  WH   ++        +A
Sbjct: 337 NLPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVLLAPNGHGTRLA 396

Query: 431 QAAAIRIEIRGGKRKKAYLQMDGEPWKQQISKDSST-FVEIKRVPFQSLMINGD 473
           QA  IR E   G     +++MDGEPWKQ +  D  T  VEI  +   +++   D
Sbjct: 397 QANRIRFEFHKGATDHTFMRMDGEPWKQPLPLDDETVMVEISHLGQVNMLATHD 435

The following BLAST results are available for this feature:

BLAST of MS002280 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134720.1	8.6e-278	99.16	diacylglycerol kinase 7-like [Momordica charantia]	[more]
XP_023516209.1	1.2e-242	86.89	diacylglycerol kinase 7-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_023516208.1	2.6e-242	86.53	diacylglycerol kinase 7-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
KAG6589732.1	3.4e-242	86.68	Diacylglycerol kinase 7, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022921758.1	1.3e-241	86.89	diacylglycerol kinase 7-like isoform X2 [Cucurbita moschata]	[more]

BLAST of MS002280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JQ95	2.0e-189	66.95	Diacylglycerol kinase 7 OS=Arabidopsis thaliana OX=3702 GN=DGK7 PE=1 SV=1	[more]
Q8VZG1	5.2e-185	64.78	Diacylglycerol kinase 3 OS=Arabidopsis thaliana OX=3702 GN=DGK3 PE=1 SV=1	[more]
Q1PDI2	2.0e-181	64.54	Diacylglycerol kinase 4 OS=Arabidopsis thaliana OX=3702 GN=DGK4 PE=1 SV=1	[more]
F4JKI3	5.0e-71	36.34	Diacylglycerol kinase 6 OS=Arabidopsis thaliana OX=3702 GN=DGK6 PE=3 SV=1	[more]
Q7XQT2	1.1e-70	36.08	Diacylglycerol kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DGK1 PE=1 SV...	[more]

BLAST of MS002280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BZ41	4.2e-278	99.16	Diacylglycerol kinase OS=Momordica charantia OX=3673 GN=LOC111006923 PE=3 SV=1	[more]
A0A6J1E4R5	6.3e-242	86.89	diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111429914 PE=3 SV=1	[more]
A0A6J1E1C6	1.4e-241	86.53	diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111429914 PE=3 SV=1	[more]
A0A6J1JHE1	2.2e-239	85.62	diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111485730 PE=3 SV=1	[more]
A0A6J1JJR6	5.0e-239	85.26	diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111485730 PE=3 SV=1	[more]

BLAST of MS002280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G30340.1	1.5e-190	66.95	diacylglycerol kinase 7	[more]
AT2G18730.1	3.7e-186	64.78	diacylglycerol kinase 3	[more]
AT5G57690.1	1.5e-182	64.54	diacylglycerol kinase 4	[more]
AT4G28130.1	3.6e-72	36.34	diacylglycerol kinase 6	[more]
AT2G20900.1	2.0e-70	35.75	diacylglycerol kinase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001206	Diacylglycerol kinase, catalytic domain	SMART	SM00046	dagk_c4a_7	coord: 74..220 e-value: 7.0E-27 score: 105.3
IPR001206	Diacylglycerol kinase, catalytic domain	PFAM	PF00781	DAGK_cat	coord: 74..213 e-value: 4.4E-30 score: 103.9
IPR001206	Diacylglycerol kinase, catalytic domain	PROSITE	PS50146	DAGK	coord: 70..227 score: 29.788589
IPR000756	Diacylglycerol kinase, accessory domain	SMART	SM00045	dagk_c4b_2	coord: 267..444 e-value: 6.5E-9 score: 30.1
IPR000756	Diacylglycerol kinase, accessory domain	PFAM	PF00609	DAGK_acc	coord: 267..444 e-value: 5.5E-42 score: 143.8
IPR016961	Diacylglycerol kinase, plant	PIRSF	PIRSF030829	DAG_kinase_plant	coord: 2..472 e-value: 3.2E-211 score: 700.0
None	No IPR available	GENE3D	2.60.200.40		coord: 263..450 e-value: 8.9E-7 score: 30.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 227..254
None	No IPR available	PANTHER	PTHR11255:SF96	DIACYLGLYCEROL KINASE	coord: 3..471
IPR017438	Inorganic polyphosphate/ATP-NAD kinase, N-terminal	GENE3D	3.40.50.10330		coord: 72..223 e-value: 2.9E-23 score: 83.8
IPR037607	Diacylglycerol kinase	PANTHER	PTHR11255	DIACYLGLYCEROL KINASE	coord: 3..471
IPR016064	NAD kinase/diacylglycerol kinase-like domain superfamily	SUPERFAMILY	111331	NAD kinase/diacylglycerol kinase-like	coord: 72..446

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS002280.1	MS002280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007205	protein kinase C-activating G protein-coupled receptor signaling pathway
biological_process	GO:0007165	signal transduction
molecular_function	GO:0004143	diacylglycerol kinase activity
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0003951	NAD+ kinase activity