MS002274 (gene) Bitter gourd (TR) v1

Overview


Name	MS002274
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Protein kinase domain-containing protein
Location	scaffold30: 3761810 .. 3763046 (-)
RNA-Seq Expression	MS002274
Synteny	MS002274

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGATTTTTACGTACAAGGAGTTGCATACGGCTACGAACGGTTTCCATGAGGACAACAAACTCGGGGAAGGAGGATTTGGAAGTGTTTACTGGGGAAAAACAAGCGATGGGCTGCAGGTAAATTGCCATAAAAATAATGTAAATGAAAGAAAAAGAAAAAAAAAAAAACAGAAGATGAATTTTTGATAATTGGGTTTGTGGCAGATAGCGGTGAAGAAGCTGAAAGCGATGAATTATAAGGCAGAAATGGAGTTTGCAGTGGAAGTGGAGGTGCTTGCGAGGCTAAGGCACAAGAACTTGTTGGGTTTAAGGGGTTACTGTGTGGGGACTGACCAGAGGCTTATAGTTTATGATTACATGCCAAATCTGAGCTTACTGTCTCATCTCCATGGCCAATTTGCTGCTGAAGCTCAATTGGATTGGAAAACAAGAATCAAGATCGCTCTTGGCTCTGCGCAAGGCATTATGTAAGTATTTTCAGTCCTCTGAAATTTTCCTACGATTATATTGTGTCAATTATTTCTTTGTTTTTAGAAAATAAACTTTTTTAAATCAATCTTTGTTTCTCTGTTTTTTGTATATGAGTTGAAAAGGTTCATCGAATTTCAGTTAAGTTTTTATAAAGCTAAAATTAGCTAGATTTTAAAACATGTTTTTATTTGAGGCAAAAGAAAATTTTACAAAACTTGCATTATCAAACAGAAAATATCTTATCCTTTATCCTTTAGAGGTCGTAGAAAGTTTAGAATCTTAATGTGTTTGAAAAAAAAAAAAGAAAAGAAAAATTTGTGACTTTTTTTAAAAATACACATTAAATTTATGCTATAGAGTAAAATCTTCTAGATAAATATAAAAGTAAAATTAGTATTAAACAAATATTTGTAGGTTCCAATCTCAATCTGTATTGAATTCTATTCTTCGATTGGATGTTCATTTGAGTTTTTGGATTCTGATATTATGTTAATATTCATGATTTCCATCTTAAAACCAATTCAATAGGATAGGAATACCTCGCTTATATTATAAACTTTTGTTGGTCATTCTATCATAATGTCAAATACTTAACACGGAGAACAAAAATTGAATAGGTTGATGTTGTTAATTATGTAGGTATTTGCACCAAGAGGTAACACCCCACATAATCCACAGAGATATCAAAGCAAGCAACATTTTGTTGGATTCAGATTTTGAGCCACTTGTAGCTGATTTTGGGTTTGCAAAACTAATACCAGAGGGC

mRNA sequence

AGGATTTTTACGTACAAGGAGTTGCATACGGCTACGAACGGTTTCCATGAGGACAACAAACTCGGGGAAGGAGGATTTGGAAGTGTTTACTGGGGAAAAACAAGCGATGGGCTGCAGATAGCGGTGAAGAAGCTGAAAGCGATGAATTATAAGGCAGAAATGGAGTTTGCAGTGGAAGTGGAGGTGCTTGCGAGGCTAAGGCACAAGAACTTGTTGGGTTTAAGGGGTTACTGTGTGGGGACTGACCAGAGGCTTATAGTTTATGATTACATGCCAAATCTGAGCTTACTGTCTCATCTCCATGGCCAATTTGCTGCTGAAGCTCAATTGGATTGGAAAACAAGAATCAAGATCGCTCTTGGCTCTGCGCAAGGCATTATGTATTTGCACCAAGAGGTAACACCCCACATAATCCACAGAGATATCAAAGCAAGCAACATTTTGTTGGATTCAGATTTTGAGCCACTTGTAGCTGATTTTGGGTTTGCAAAACTAATACCAGAGGGC

Coding sequence (CDS)

AGGATTTTTACGTACAAGGAGTTGCATACGGCTACGAACGGTTTCCATGAGGACAACAAACTCGGGGAAGGAGGATTTGGAAGTGTTTACTGGGGAAAAACAAGCGATGGGCTGCAGATAGCGGTGAAGAAGCTGAAAGCGATGAATTATAAGGCAGAAATGGAGTTTGCAGTGGAAGTGGAGGTGCTTGCGAGGCTAAGGCACAAGAACTTGTTGGGTTTAAGGGGTTACTGTGTGGGGACTGACCAGAGGCTTATAGTTTATGATTACATGCCAAATCTGAGCTTACTGTCTCATCTCCATGGCCAATTTGCTGCTGAAGCTCAATTGGATTGGAAAACAAGAATCAAGATCGCTCTTGGCTCTGCGCAAGGCATTATGTATTTGCACCAAGAGGTAACACCCCACATAATCCACAGAGATATCAAAGCAAGCAACATTTTGTTGGATTCAGATTTTGAGCCACTTGTAGCTGATTTTGGGTTTGCAAAACTAATACCAGAGGGC

Protein sequence

RIFTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEVEVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIALGSAQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKLIPEG

Homology

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BLAST of MS002274 vs. NCBI nr
Match: XP_022135413.1 (PTI1-like tyrosine-protein kinase At3g15890 [Momordica charantia])

HSP 1 Score: 348.2 bits (892), Expect = 4.0e-92
Identity = 169/169 (100.00%), Postives = 169/169 (100.00%), Query Frame = 0


Query: 1   RIFTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV 60
           RIFTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV
Sbjct: 27  RIFTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV 86

Query: 61  EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIAL 120
           EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIAL
Sbjct: 87  EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIAL 146

Query: 121 GSAQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKLIPEG 170
           GSAQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKLIPEG
Sbjct: 147 GSAQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKLIPEG 195

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BLAST of MS002274 vs. NCBI nr
Match: XP_022988518.1 (PTI1-like tyrosine-protein kinase At3g15890 [Cucurbita maxima])

HSP 1 Score: 334.7 bits (857), Expect = 4.5e-88
Identity = 160/169 (94.67%), Postives = 165/169 (97.63%), Query Frame = 0


Query: 1   RIFTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV 60
           R+FTYKELH ATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV
Sbjct: 25  RMFTYKELHAATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV 84

Query: 61  EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIAL 120
           EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFA+EA LDWK R+KIAL
Sbjct: 85  EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFASEAHLDWKRRMKIAL 144

Query: 121 GSAQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKLIPEG 170
           GSAQGI+YLHQEV PHIIHRDIKASN+LLDSDFEPLVADFGFAKLIPEG
Sbjct: 145 GSAQGILYLHQEVRPHIIHRDIKASNVLLDSDFEPLVADFGFAKLIPEG 193

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BLAST of MS002274 vs. NCBI nr
Match: KAG6589728.1 (PTI1-like tyrosine-protein kinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 333.6 bits (854), Expect = 1.0e-87
Identity = 159/169 (94.08%), Postives = 165/169 (97.63%), Query Frame = 0


Query: 1   RIFTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV 60
           R+FTYKELH ATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV
Sbjct: 25  RMFTYKELHAATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV 84

Query: 61  EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIAL 120
           EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFA+EA LDWK R+KIAL
Sbjct: 85  EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFASEAHLDWKRRMKIAL 144

Query: 121 GSAQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKLIPEG 170
           GSA+GI+YLHQEV PHIIHRDIKASN+LLDSDFEPLVADFGFAKLIPEG
Sbjct: 145 GSAEGILYLHQEVRPHIIHRDIKASNVLLDSDFEPLVADFGFAKLIPEG 193

[Close]

BLAST of MS002274 vs. NCBI nr
Match: XP_023516727.1 (PTI1-like tyrosine-protein kinase At3g15890 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 333.6 bits (854), Expect = 1.0e-87
Identity = 159/169 (94.08%), Postives = 165/169 (97.63%), Query Frame = 0


Query: 1   RIFTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV 60
           R+FTYKELH ATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV
Sbjct: 25  RMFTYKELHAATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV 84

Query: 61  EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIAL 120
           EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFA+EA LDWK R+KIAL
Sbjct: 85  EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFASEAHLDWKRRMKIAL 144

Query: 121 GSAQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKLIPEG 170
           GSA+GI+YLHQEV PHIIHRDIKASN+LLDSDFEPLVADFGFAKLIPEG
Sbjct: 145 GSAEGILYLHQEVRPHIIHRDIKASNVLLDSDFEPLVADFGFAKLIPEG 193

[Close]

BLAST of MS002274 vs. NCBI nr
Match: KAG7023405.1 (PTI1-like tyrosine-protein kinase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 333.6 bits (854), Expect = 1.0e-87
Identity = 159/169 (94.08%), Postives = 165/169 (97.63%), Query Frame = 0


Query: 1   RIFTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV 60
           R+FTYKELH ATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV
Sbjct: 25  RMFTYKELHAATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV 84

Query: 61  EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIAL 120
           EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFA+EA LDWK R+KIAL
Sbjct: 85  EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFASEAHLDWKRRMKIAL 144

Query: 121 GSAQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKLIPEG 170
           GSA+GI+YLHQEV PHIIHRDIKASN+LLDSDFEPLVADFGFAKLIPEG
Sbjct: 145 GSAEGILYLHQEVRPHIIHRDIKASNVLLDSDFEPLVADFGFAKLIPEG 193

[Close]

BLAST of MS002274 vs. ExPASy Swiss-Prot
Match: Q9LSC2 (PTI1-like tyrosine-protein kinase At3g15890 OS=Arabidopsis thaliana OX=3702 GN=At3g15890 PE=2 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 4.9e-61
Identity = 107/168 (63.69%), Postives = 134/168 (79.76%), Query Frame = 0


Query: 1   RIFTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV 60
           R+F+ KELH ATN F+ DNKLGEG FGSVYWG+  DG QIAVK+LK  + + E++FAVEV
Sbjct: 25  RVFSLKELHAATNSFNYDNKLGEGRFGSVYWGQLWDGSQIAVKRLKEWSNREEIDFAVEV 84

Query: 61  EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIAL 120
           E+LAR+RHKNLL +RGYC    +RL+VY+YM NLSL+SHLHGQ +AE  LDW  R+KIA+
Sbjct: 85  EILARIRHKNLLSVRGYCAEGQERLLVYEYMQNLSLVSHLHGQHSAECLLDWTKRMKIAI 144

Query: 121 GSAQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKLIPE 169
            SAQ I YLH   TPHI+H D++ASN+LLDS+FE  V DFG+ KL+P+
Sbjct: 145 SSAQAIAYLHDHATPHIVHGDVRASNVLLDSEFEARVTDFGYGKLMPD 192

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BLAST of MS002274 vs. ExPASy Swiss-Prot
Match: Q9LV48 (Proline-rich receptor-like protein kinase PERK1 OS=Arabidopsis thaliana OX=3702 GN=PERK1 PE=1 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 5.8e-46
Identity = 89/163 (54.60%), Postives = 120/163 (73.62%), Query Frame = 0


Query: 3   FTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEVEV 62
           FTY+EL  ATNGF E N LG+GGFG V+ G    G ++AVK+LKA + + E EF  EVE+
Sbjct: 268 FTYEELSRATNGFSEANLLGQGGFGYVHKGILPSGKEVAVKQLKAGSGQGEREFQAEVEI 327

Query: 63  LARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIALGS 122
           ++R+ H++L+ L GYC+   QRL+VY+++PN +L  HLHG+      ++W TR+KIALGS
Sbjct: 328 ISRVHHRHLVSLIGYCMAGVQRLLVYEFVPNNNLEFHLHGK--GRPTMEWSTRLKIALGS 387

Query: 123 AQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKL 166
           A+G+ YLH++  P IIHRDIKASNIL+D  FE  VADFG AK+
Sbjct: 388 AKGLSYLHEDCNPKIIHRDIKASNILIDFKFEAKVADFGLAKI 428

[Close]

BLAST of MS002274 vs. ExPASy Swiss-Prot
Match: Q9FFW5 (Proline-rich receptor-like protein kinase PERK8 OS=Arabidopsis thaliana OX=3702 GN=PERK8 PE=1 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 5.8e-46
Identity = 88/166 (53.01%), Postives = 117/166 (70.48%), Query Frame = 0


Query: 3   FTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEVEV 62
           F+Y EL   T+GF E N LGEGGFG VY G  SDG ++AVK+LK    + E EF  EVE+
Sbjct: 327 FSYDELSQVTSGFSEKNLLGEGGFGCVYKGVLSDGREVAVKQLKIGGSQGEREFKAEVEI 386

Query: 63  LARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIALGS 122
           ++R+ H++L+ L GYC+    RL+VYDY+PN +L  HLH        + W+TR+++A G+
Sbjct: 387 ISRVHHRHLVTLVGYCISEQHRLLVYDYVPNNTLHYHLHA--PGRPVMTWETRVRVAAGA 446

Query: 123 AQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKLIPE 169
           A+GI YLH++  P IIHRDIK+SNILLD+ FE LVADFG AK+  E
Sbjct: 447 ARGIAYLHEDCHPRIIHRDIKSSNILLDNSFEALVADFGLAKIAQE 490

[Close]

BLAST of MS002274 vs. ExPASy Swiss-Prot
Match: Q9C660 (Proline-rich receptor-like protein kinase PERK10 OS=Arabidopsis thaliana OX=3702 GN=PERK10 PE=1 SV=2)

HSP 1 Score: 184.1 bits (466), Expect = 1.3e-45
Identity = 86/164 (52.44%), Postives = 119/164 (72.56%), Query Frame = 0


Query: 2   IFTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEVE 61
           +F+Y+EL  ATNGF ++N LGEGGFG VY G   D   +AVK+LK    + + EF  EV+
Sbjct: 417 LFSYEELVIATNGFSDENLLGEGGFGRVYKGVLPDERVVAVKQLKIGGGQGDREFKAEVD 476

Query: 62  VLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIALG 121
            ++R+ H+NLL + GYC+  ++RL++YDY+PN +L  HLH   A    LDW TR+KIA G
Sbjct: 477 TISRVHHRNLLSMVGYCISENRRLLIYDYVPNNNLYFHLHA--AGTPGLDWATRVKIAAG 536

Query: 122 SAQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKL 166
           +A+G+ YLH++  P IIHRDIK+SNILL+++F  LV+DFG AKL
Sbjct: 537 AARGLAYLHEDCHPRIIHRDIKSSNILLENNFHALVSDFGLAKL 578

[Close]

BLAST of MS002274 vs. ExPASy Swiss-Prot
Match: Q9CAL8 (Proline-rich receptor-like protein kinase PERK13 OS=Arabidopsis thaliana OX=3702 GN=PERK13 PE=1 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 1.3e-45
Identity = 85/163 (52.15%), Postives = 119/163 (73.01%), Query Frame = 0


Query: 3   FTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEVEV 62
           FTY+EL   T GF + N LGEGGFG VY GK +DG  +AVK+LK  + + + EF  EVE+
Sbjct: 341 FTYEELTDITEGFSKHNILGEGGFGCVYKGKLNDGKLVAVKQLKVGSGQGDREFKAEVEI 400

Query: 63  LARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIALGS 122
           ++R+ H++L+ L GYC+   +RL++Y+Y+PN +L  HLHG+      L+W  R++IA+GS
Sbjct: 401 ISRVHHRHLVSLVGYCIADSERLLIYEYVPNQTLEHHLHGK--GRPVLEWARRVRIAIGS 460

Query: 123 AQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKL 166
           A+G+ YLH++  P IIHRDIK++NILLD +FE  VADFG AKL
Sbjct: 461 AKGLAYLHEDCHPKIIHRDIKSANILLDDEFEAQVADFGLAKL 501

[Close]

BLAST of MS002274 vs. ExPASy TrEMBL
Match: A0A6J1C4R2 (PTI1-like tyrosine-protein kinase At3g15890 OS=Momordica charantia OX=3673 GN=LOC111007371 PE=3 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 1.9e-92
Identity = 169/169 (100.00%), Postives = 169/169 (100.00%), Query Frame = 0


Query: 1   RIFTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV 60
           RIFTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV
Sbjct: 27  RIFTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV 86

Query: 61  EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIAL 120
           EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIAL
Sbjct: 87  EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIAL 146

Query: 121 GSAQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKLIPEG 170
           GSAQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKLIPEG
Sbjct: 147 GSAQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKLIPEG 195

[Close]

BLAST of MS002274 vs. ExPASy TrEMBL
Match: A0A6J1JMI5 (PTI1-like tyrosine-protein kinase At3g15890 OS=Cucurbita maxima OX=3661 GN=LOC111485734 PE=3 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 2.2e-88
Identity = 160/169 (94.67%), Postives = 165/169 (97.63%), Query Frame = 0


Query: 1   RIFTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV 60
           R+FTYKELH ATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV
Sbjct: 25  RMFTYKELHAATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV 84

Query: 61  EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIAL 120
           EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFA+EA LDWK R+KIAL
Sbjct: 85  EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFASEAHLDWKRRMKIAL 144

Query: 121 GSAQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKLIPEG 170
           GSAQGI+YLHQEV PHIIHRDIKASN+LLDSDFEPLVADFGFAKLIPEG
Sbjct: 145 GSAQGILYLHQEVRPHIIHRDIKASNVLLDSDFEPLVADFGFAKLIPEG 193

[Close]

BLAST of MS002274 vs. ExPASy TrEMBL
Match: A0A6J1E5G5 (PTI1-like tyrosine-protein kinase At3g15890 OS=Cucurbita moschata OX=3662 GN=LOC111429664 PE=3 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 4.9e-88
Identity = 159/169 (94.08%), Postives = 165/169 (97.63%), Query Frame = 0


Query: 1   RIFTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV 60
           R+FTYKELH ATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV
Sbjct: 25  RMFTYKELHAATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV 84

Query: 61  EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIAL 120
           EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFA+EA LDWK R+KIAL
Sbjct: 85  EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFASEAHLDWKRRMKIAL 144

Query: 121 GSAQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKLIPEG 170
           GSA+GI+YLHQEV PHIIHRDIKASN+LLDSDFEPLVADFGFAKLIPEG
Sbjct: 145 GSAEGILYLHQEVRPHIIHRDIKASNVLLDSDFEPLVADFGFAKLIPEG 193

[Close]

BLAST of MS002274 vs. ExPASy TrEMBL
Match: A0A1S3BWV2 (PTI1-like tyrosine-protein kinase At3g15890 OS=Cucumis melo OX=3656 GN=LOC103494300 PE=3 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 1.2e-86
Identity = 156/169 (92.31%), Postives = 164/169 (97.04%), Query Frame = 0


Query: 1   RIFTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV 60
           R++TYKELH ATNGFH+DNKLGEGGFGSVYWGKT+DGLQIAVKKLKAMNYKAEMEFAVEV
Sbjct: 29  RMYTYKELHAATNGFHDDNKLGEGGFGSVYWGKTTDGLQIAVKKLKAMNYKAEMEFAVEV 88

Query: 61  EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIAL 120
           EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHG FA+EA LDWK R+KIAL
Sbjct: 89  EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGHFASEALLDWKRRLKIAL 148

Query: 121 GSAQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKLIPEG 170
           GSAQGI+YLHQEV PHIIHRDIKASN+LLDSDFEPLVADFGFAKLIPEG
Sbjct: 149 GSAQGILYLHQEVKPHIIHRDIKASNVLLDSDFEPLVADFGFAKLIPEG 197

[Close]

BLAST of MS002274 vs. ExPASy TrEMBL
Match: A0A2C9VM87 (Protein kinase domain-containing protein OS=Manihot esculenta OX=3983 GN=MANES_06G021900 PE=3 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 8.7e-85
Identity = 153/169 (90.53%), Postives = 161/169 (95.27%), Query Frame = 0


Query: 1   RIFTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV 60
           RIFTYKELH ATNGF EDNKLGEGGFGSVYWGKT+DGLQIAVKKLKAMN KAEMEFAVEV
Sbjct: 28  RIFTYKELHAATNGFSEDNKLGEGGFGSVYWGKTTDGLQIAVKKLKAMNSKAEMEFAVEV 87

Query: 61  EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIAL 120
           EVL R+RHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFA E QLDWK R+KIA+
Sbjct: 88  EVLGRVRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAGEVQLDWKRRMKIAI 147

Query: 121 GSAQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKLIPEG 170
           GSA+G++YLH EVTPHIIHRDIKASN+LLDSDFEPLVADFGFAKLIPEG
Sbjct: 148 GSAEGLLYLHHEVTPHIIHRDIKASNVLLDSDFEPLVADFGFAKLIPEG 196

[Close]

BLAST of MS002274 vs. TAIR 10
Match: AT3G15890.1 (Protein kinase superfamily protein )

HSP 1 Score: 235.3 bits (599), Expect = 3.5e-62
Identity = 107/168 (63.69%), Postives = 134/168 (79.76%), Query Frame = 0


Query: 1   RIFTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV 60
           R+F+ KELH ATN F+ DNKLGEG FGSVYWG+  DG QIAVK+LK  + + E++FAVEV
Sbjct: 25  RVFSLKELHAATNSFNYDNKLGEGRFGSVYWGQLWDGSQIAVKRLKEWSNREEIDFAVEV 84

Query: 61  EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIAL 120
           E+LAR+RHKNLL +RGYC    +RL+VY+YM NLSL+SHLHGQ +AE  LDW  R+KIA+
Sbjct: 85  EILARIRHKNLLSVRGYCAEGQERLLVYEYMQNLSLVSHLHGQHSAECLLDWTKRMKIAI 144

Query: 121 GSAQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKLIPE 169
            SAQ I YLH   TPHI+H D++ASN+LLDS+FE  V DFG+ KL+P+
Sbjct: 145 SSAQAIAYLHDHATPHIVHGDVRASNVLLDSEFEARVTDFGYGKLMPD 192

[Close]

BLAST of MS002274 vs. TAIR 10
Match: AT1G52540.1 (Protein kinase superfamily protein )

HSP 1 Score: 235.0 bits (598), Expect = 4.6e-62
Identity = 109/168 (64.88%), Postives = 135/168 (80.36%), Query Frame = 0


Query: 1   RIFTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEV 60
           RIF+ KELH ATN F+ DNKLGEG FGSVYWG+  DG QIAVK+LKA + + E++FAVEV
Sbjct: 26  RIFSLKELHAATNSFNYDNKLGEGRFGSVYWGQLWDGSQIAVKRLKAWSSREEIDFAVEV 85

Query: 61  EVLARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIAL 120
           E+LAR+RHKNLL +RGYC    +RLIVYDYMPNLSL+SHLHGQ ++E+ LDW  R+ IA+
Sbjct: 86  EILARIRHKNLLSVRGYCAEGQERLIVYDYMPNLSLVSHLHGQHSSESLLDWTRRMNIAV 145

Query: 121 GSAQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKLIPE 169
            SAQ I YLH   TP I+H D++ASN+LLDS+FE  V DFG+ KL+P+
Sbjct: 146 SSAQAIAYLHHFATPRIVHGDVRASNVLLDSEFEARVTDFGYDKLMPD 193

[Close]

BLAST of MS002274 vs. TAIR 10
Match: AT3G24550.1 (proline extensin-like receptor kinase 1 )

HSP 1 Score: 185.3 bits (469), Expect = 4.1e-47
Identity = 89/163 (54.60%), Postives = 120/163 (73.62%), Query Frame = 0


Query: 3   FTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEVEV 62
           FTY+EL  ATNGF E N LG+GGFG V+ G    G ++AVK+LKA + + E EF  EVE+
Sbjct: 268 FTYEELSRATNGFSEANLLGQGGFGYVHKGILPSGKEVAVKQLKAGSGQGEREFQAEVEI 327

Query: 63  LARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIALGS 122
           ++R+ H++L+ L GYC+   QRL+VY+++PN +L  HLHG+      ++W TR+KIALGS
Sbjct: 328 ISRVHHRHLVSLIGYCMAGVQRLLVYEFVPNNNLEFHLHGK--GRPTMEWSTRLKIALGS 387

Query: 123 AQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKL 166
           A+G+ YLH++  P IIHRDIKASNIL+D  FE  VADFG AK+
Sbjct: 388 AKGLSYLHEDCNPKIIHRDIKASNILIDFKFEAKVADFGLAKI 428

[Close]

BLAST of MS002274 vs. TAIR 10
Match: AT5G38560.1 (Protein kinase superfamily protein )

HSP 1 Score: 185.3 bits (469), Expect = 4.1e-47
Identity = 88/166 (53.01%), Postives = 117/166 (70.48%), Query Frame = 0


Query: 3   FTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEVEV 62
           F+Y EL   T+GF E N LGEGGFG VY G  SDG ++AVK+LK    + E EF  EVE+
Sbjct: 327 FSYDELSQVTSGFSEKNLLGEGGFGCVYKGVLSDGREVAVKQLKIGGSQGEREFKAEVEI 386

Query: 63  LARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIALGS 122
           ++R+ H++L+ L GYC+    RL+VYDY+PN +L  HLH        + W+TR+++A G+
Sbjct: 387 ISRVHHRHLVTLVGYCISEQHRLLVYDYVPNNTLHYHLHA--PGRPVMTWETRVRVAAGA 446

Query: 123 AQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKLIPE 169
           A+GI YLH++  P IIHRDIK+SNILLD+ FE LVADFG AK+  E
Sbjct: 447 ARGIAYLHEDCHPRIIHRDIKSSNILLDNSFEALVADFGLAKIAQE 490

[Close]

BLAST of MS002274 vs. TAIR 10
Match: AT1G70460.1 (root hair specific 10 )

HSP 1 Score: 184.1 bits (466), Expect = 9.2e-47
Identity = 85/163 (52.15%), Postives = 119/163 (73.01%), Query Frame = 0


Query: 3   FTYKELHTATNGFHEDNKLGEGGFGSVYWGKTSDGLQIAVKKLKAMNYKAEMEFAVEVEV 62
           FTY+EL   T GF + N LGEGGFG VY GK +DG  +AVK+LK  + + + EF  EVE+
Sbjct: 341 FTYEELTDITEGFSKHNILGEGGFGCVYKGKLNDGKLVAVKQLKVGSGQGDREFKAEVEI 400

Query: 63  LARLRHKNLLGLRGYCVGTDQRLIVYDYMPNLSLLSHLHGQFAAEAQLDWKTRIKIALGS 122
           ++R+ H++L+ L GYC+   +RL++Y+Y+PN +L  HLHG+      L+W  R++IA+GS
Sbjct: 401 ISRVHHRHLVSLVGYCIADSERLLIYEYVPNQTLEHHLHGK--GRPVLEWARRVRIAIGS 460

Query: 123 AQGIMYLHQEVTPHIIHRDIKASNILLDSDFEPLVADFGFAKL 166
           A+G+ YLH++  P IIHRDIK++NILLD +FE  VADFG AKL
Sbjct: 461 AKGLAYLHEDCHPKIIHRDIKSANILLDDEFEAQVADFGLAKL 501

The following BLAST results are available for this feature:

BLAST of MS002274 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135413.1	4.0e-92	100.00	PTI1-like tyrosine-protein kinase At3g15890 [Momordica charantia]	[more]
XP_022988518.1	4.5e-88	94.67	PTI1-like tyrosine-protein kinase At3g15890 [Cucurbita maxima]	[more]
KAG6589728.1	1.0e-87	94.08	PTI1-like tyrosine-protein kinase, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023516727.1	1.0e-87	94.08	PTI1-like tyrosine-protein kinase At3g15890 [Cucurbita pepo subsp. pepo]	[more]
KAG7023405.1	1.0e-87	94.08	PTI1-like tyrosine-protein kinase, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of MS002274 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSC2	4.9e-61	63.69	PTI1-like tyrosine-protein kinase At3g15890 OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
Q9LV48	5.8e-46	54.60	Proline-rich receptor-like protein kinase PERK1 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9FFW5	5.8e-46	53.01	Proline-rich receptor-like protein kinase PERK8 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9C660	1.3e-45	52.44	Proline-rich receptor-like protein kinase PERK10 OS=Arabidopsis thaliana OX=3702...	[more]
Q9CAL8	1.3e-45	52.15	Proline-rich receptor-like protein kinase PERK13 OS=Arabidopsis thaliana OX=3702...	[more]

BLAST of MS002274 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C4R2	1.9e-92	100.00	PTI1-like tyrosine-protein kinase At3g15890 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1JMI5	2.2e-88	94.67	PTI1-like tyrosine-protein kinase At3g15890 OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1E5G5	4.9e-88	94.08	PTI1-like tyrosine-protein kinase At3g15890 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A1S3BWV2	1.2e-86	92.31	PTI1-like tyrosine-protein kinase At3g15890 OS=Cucumis melo OX=3656 GN=LOC103494...	[more]
A0A2C9VM87	8.7e-85	90.53	Protein kinase domain-containing protein OS=Manihot esculenta OX=3983 GN=MANES_0...	[more]

BLAST of MS002274 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15890.1	3.5e-62	63.69	Protein kinase superfamily protein	[more]
AT1G52540.1	4.6e-62	64.88	Protein kinase superfamily protein	[more]
AT3G24550.1	4.1e-47	54.60	proline extensin-like receptor kinase 1	[more]
AT5G38560.1	4.1e-47	53.01	Protein kinase superfamily protein	[more]
AT1G70460.1	9.2e-47	52.15	root hair specific 10	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 15..168 e-value: 9.8E-6 score: -54.1
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 15..167 e-value: 1.2E-29 score: 103.5
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 15..169 score: 27.679331
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 91..169 e-value: 8.3E-24 score: 86.5
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 1..90 e-value: 4.9E-31 score: 108.9
None	No IPR available	PIRSR	PIRSR038172-1	PIRSR038172-1	coord: 19..164 e-value: 4.3E-10 score: 36.4
None	No IPR available	PIRSR	PIRSR000605-51	PIRSR000605-51	coord: 14..164 e-value: 5.3E-5 score: 20.0
None	No IPR available	PANTHER	PTHR27001	OS01G0253100 PROTEIN	coord: 1..169
None	No IPR available	PANTHER	PTHR27001:SF178	OS06G0654600 PROTEIN	coord: 1..169
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 137..149
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 21..44
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 2..168

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS002274.1	MS002274.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity