MS002270 (gene) Bitter gourd (TR) v1

BLAST of MS002270 vs. NCBI nr
Match: XP_022135222.1 (putative lipase YDR444W [Momordica charantia])

HSP 1 Score: 650.2 bits (1676), Expect = 9.3e-183
Identity = 318/321 (99.07%), Postives = 321/321 (100.00%), Query Frame = 0

Query: 1   MVAANTPLHTPPSGPATAHHPSTTLSRHCSSNPFARFRPKPLPDPAAALLLSHRPKMDLP 60
           MVAANTPLHTPPSGPATAHHPSTTLSRHCSSNPFARFRPKPLPDPAAALLLSHRPKMDLP
Sbjct: 1   MVAANTPLHTPPSGPATAHHPSTTLSRHCSSNPFARFRPKPLPDPAAALLLSHRPKMDLP 60

Query: 61  RRVAAPCFGSRRSKGLKVDPDFGGQEIFYADAKAAPEHLVIMVNGLIGSAADWRYAAGQF 120
           RRVAAPCFGSRRSKGLKVDPDFGGQEIFYADAKAAPEHLVIMVNGLIGSAADWRYAAGQF
Sbjct: 61  RRVAAPCFGSRRSKGLKVDPDFGGQEIFYADAKAAPEHLVIMVNGLIGSAADWRYAAGQF 120

Query: 121 VKKLPDKVIVHRNECNSSRLTFDGVDTMGERLAKEVLAVVRCRPEVQKISFVAHSLGGLV 180
           VKKLPDKVIVHR+ECNSSRLTFDGVDTMGERLAKEVLAVVRCRPEVQKISFVAHSLGGLV
Sbjct: 121 VKKLPDKVIVHRSECNSSRLTFDGVDTMGERLAKEVLAVVRCRPEVQKISFVAHSLGGLV 180

Query: 181 ARYAIGRLFHHIPRLESSGAPENSSAEEQKQHLEQFHHARIAGLEPVNFITFATPHLGSR 240
           ARYAIGRLFHHIPRLESSGAPENSSAEEQKQHLEQFHHARIAGLEPVNFITFATPHLGSR
Sbjct: 181 ARYAIGRLFHHIPRLESSGAPENSSAEEQKQHLEQFHHARIAGLEPVNFITFATPHLGSR 240

Query: 241 GNKQFPVLGGLPFLERGASQTAHLVAGRSGKHLFLTDDDDDGKPPLLLRMVTDAEDLKFI 300
           GNKQFPVLGGLPFLERGASQTAHLVAGRSGKHLFLTDDDDDGKPPLLLRMVTDAEDLKFI
Sbjct: 241 GNKQFPVLGGLPFLERGASQTAHLVAGRSGKHLFLTDDDDDGKPPLLLRMVTDAEDLKFI 300

Query: 301 SALRSFKRRVAYANVNYDHIL 322
           SALRSFKRRVAYANVNYDH++
Sbjct: 301 SALRSFKRRVAYANVNYDHMV 321

BLAST of MS002270 vs. NCBI nr
Match: KAG6589725.1 (hypothetical protein SDJN03_15148, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023402.1 hypothetical protein SDJN02_14427 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 498.8 bits (1283), Expect = 3.5e-137
Identity = 262/326 (80.37%), Postives = 280/326 (85.89%), Query Frame = 0

Query: 3   AANTPLHTPPSGPATAHHPSTTLSRHCSSNPFARFR----PKPLPDPAAALLLSHRPKMD 62
           AANTPL TPPSGP     PS T  RHC SNPF RF     PKP PDP  AL LS+  +MD
Sbjct: 5   AANTPLQTPPSGP--LFDPSAT--RHCLSNPFPRFTIRFPPKPFPDP--ALSLSNHSRMD 64

Query: 63  LPRRVAAPCFGS-RRSKGLKVDPDFGGQEIFYADAKAAPEHLVIMVNGLIGSAADWRYAA 122
           L  R+A   F S RR KGLKV+ DF G+E F  DAKAAPEHLVIMVNGLIGSAADWRYAA
Sbjct: 65  LIGRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAA 124

Query: 123 GQFVKKLPDKVIVHRNECNSSRLTFDGVDTMGERLAKEVLAVVRCRPEVQKISFVAHSLG 182
            QFV KLPD VIVHR+ECN+SRLTFDGVDTMGERLA+EVLAV+R  PEVQKISFVAHSLG
Sbjct: 125 EQFVTKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLG 184

Query: 183 GLVARYAIGRLFHHIPRLESSGAPENSSAEEQKQHLEQFHHARIAGLEPVNFITFATPHL 242
           GLVARYAIGRLF HIP+LESSGAP++ SAEEQKQ +EQFHHARIAGLEPVNFIT ATPHL
Sbjct: 185 GLVARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHL 244

Query: 243 GSRGNKQFPVLGGLPFLERGASQTAHLVAGRSGKHLFLTDDDDDGKPPLLLRMVTDAEDL 302
           GSRGNKQFPVL GLPFLER ASQTAHL+AGRSGKHLFLTDD++DGKPPLLLRMVTD+EDL
Sbjct: 245 GSRGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDL 304

Query: 303 KFISALRSFKRRVAYANVNYDHILLH 324
           KFISALR+FKRRVAYANVNYDHILLH
Sbjct: 305 KFISALRAFKRRVAYANVNYDHILLH 324

BLAST of MS002270 vs. NCBI nr
Match: XP_038879828.1 (putative lipase ROG1 isoform X1 [Benincasa hispida])

HSP 1 Score: 490.3 bits (1261), Expect = 1.2e-134
Identity = 249/320 (77.81%), Postives = 275/320 (85.94%), Query Frame = 0

Query: 3   AANTPLHTPPSGPATAHHPSTTLSRHCSSNPFA-RFRPKPLPDPAAALLLSHRPKMDLPR 62
           AANTPL+TPPSGP   H  +T   RHC SN F   F P P PDP  +L LS+  +MD   
Sbjct: 5   AANTPLYTPPSGPPLDHSSTT---RHCPSNSFTLSFPPTPFPDP--SLSLSNHSRMDFAG 64

Query: 63  RVAAPCFGSRRSKGLKVDPDFGGQEIFYADAKAAPEHLVIMVNGLIGSAADWRYAAGQFV 122
           R+A  CF +R  KGLKV+ DF  +E FY DAKA PEHLVIMVNGLIGSAADWRYAA QFV
Sbjct: 65  RLATSCFRTR--KGLKVEADFAAEEFFYPDAKAPPEHLVIMVNGLIGSAADWRYAAAQFV 124

Query: 123 KKLPDKVIVHRNECNSSRLTFDGVDTMGERLAKEVLAVVRCRPEVQKISFVAHSLGGLVA 182
           KKLPDKVIVHR+ECN+SR TFDGVDTMGERLA+EVL V+R RPE+QKISFVAHSLGGLV+
Sbjct: 125 KKLPDKVIVHRSECNTSRSTFDGVDTMGERLAEEVLGVIRRRPELQKISFVAHSLGGLVS 184

Query: 183 RYAIGRLFHHIPRLESSGAPENSSAEEQKQHLEQFHHARIAGLEPVNFITFATPHLGSRG 242
           RYAIGRLF HIP+LESSGAP++ SAEE+KQH+EQFH ARIAGLEPVNFIT ATPHLGSRG
Sbjct: 185 RYAIGRLFDHIPQLESSGAPQSFSAEEKKQHIEQFHRARIAGLEPVNFITVATPHLGSRG 244

Query: 243 NKQFPVLGGLPFLERGASQTAHLVAGRSGKHLFLTDDDDDGKPPLLLRMVTDAEDLKFIS 302
           NKQFPVL GLPFLER ASQTAHLVAGRSGKHLFLTDD++DGKPPLLLRMVTD+EDLKFIS
Sbjct: 245 NKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKFIS 304

Query: 303 ALRSFKRRVAYANVNYDHIL 322
           ALR+FKRRVAYANVNYDH++
Sbjct: 305 ALRAFKRRVAYANVNYDHMV 317

BLAST of MS002270 vs. NCBI nr
Match: XP_038879829.1 (putative lipase ROG1 isoform X2 [Benincasa hispida])

HSP 1 Score: 490.3 bits (1261), Expect = 1.2e-134
Identity = 249/320 (77.81%), Postives = 275/320 (85.94%), Query Frame = 0

Query: 3   AANTPLHTPPSGPATAHHPSTTLSRHCSSNPFA-RFRPKPLPDPAAALLLSHRPKMDLPR 62
           AANTPL+TPPSGP   H  +T   RHC SN F   F P P PDP  +L LS+  +MD   
Sbjct: 5   AANTPLYTPPSGPPLDHSSTT---RHCPSNSFTLSFPPTPFPDP--SLSLSNHSRMDFAG 64

Query: 63  RVAAPCFGSRRSKGLKVDPDFGGQEIFYADAKAAPEHLVIMVNGLIGSAADWRYAAGQFV 122
           R+A  CF +R  KGLKV+ DF  +E FY DAKA PEHLVIMVNGLIGSAADWRYAA QFV
Sbjct: 65  RLATSCFRTR--KGLKVEADFAAEEFFYPDAKAPPEHLVIMVNGLIGSAADWRYAAAQFV 124

Query: 123 KKLPDKVIVHRNECNSSRLTFDGVDTMGERLAKEVLAVVRCRPEVQKISFVAHSLGGLVA 182
           KKLPDKVIVHR+ECN+SR TFDGVDTMGERLA+EVL V+R RPE+QKISFVAHSLGGLV+
Sbjct: 125 KKLPDKVIVHRSECNTSRSTFDGVDTMGERLAEEVLGVIRRRPELQKISFVAHSLGGLVS 184

Query: 183 RYAIGRLFHHIPRLESSGAPENSSAEEQKQHLEQFHHARIAGLEPVNFITFATPHLGSRG 242
           RYAIGRLF HIP+LESSGAP++ SAEE+KQH+EQFH ARIAGLEPVNFIT ATPHLGSRG
Sbjct: 185 RYAIGRLFDHIPQLESSGAPQSFSAEEKKQHIEQFHRARIAGLEPVNFITVATPHLGSRG 244

Query: 243 NKQFPVLGGLPFLERGASQTAHLVAGRSGKHLFLTDDDDDGKPPLLLRMVTDAEDLKFIS 302
           NKQFPVL GLPFLER ASQTAHLVAGRSGKHLFLTDD++DGKPPLLLRMVTD+EDLKFIS
Sbjct: 245 NKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKFIS 304

Query: 303 ALRSFKRRVAYANVNYDHIL 322
           ALR+FKRRVAYANVNYDH++
Sbjct: 305 ALRAFKRRVAYANVNYDHMV 317

BLAST of MS002270 vs. NCBI nr
Match: XP_008453642.1 (PREDICTED: putative lipase YOR059C isoform X3 [Cucumis melo])

HSP 1 Score: 488.4 bits (1256), Expect = 4.7e-134
Identity = 251/320 (78.44%), Postives = 274/320 (85.62%), Query Frame = 0

Query: 3   AANTPLHTPPSGPATAHHPSTTLSRHCSSNPFA-RFRPKPLPDPAAALLLSHRPKMDLPR 62
           A  TPLHTPPSGP +  HPSTT   HC SNPF+ RF P P P P     L  RP+MD P 
Sbjct: 38  ATITPLHTPPSGP-SFDHPSTT--PHCPSNPFSLRFPPIPFPFPFPDPSLP-RPRMDFPG 97

Query: 63  RVAAPCFGSRRSKGLKVDPDFGGQEIFYADAKAAPEHLVIMVNGLIGSAADWRYAAGQFV 122
           R+A  CF  R  KGLKVD DF  +E FY DAKA PEHLVIMVNGLIGSAADWRYAAGQFV
Sbjct: 98  RLATGCF--RPRKGLKVDADFTAEEFFYPDAKAPPEHLVIMVNGLIGSAADWRYAAGQFV 157

Query: 123 KKLPDKVIVHRNECNSSRLTFDGVDTMGERLAKEVLAVVRCRPEVQKISFVAHSLGGLVA 182
           KKLPDKVIVHR+ECNSSR TFDGVDTMGERLA+EVL V+R RPE+QKISFVAHSLGGLVA
Sbjct: 158 KKLPDKVIVHRSECNSSRSTFDGVDTMGERLAEEVLGVIRRRPELQKISFVAHSLGGLVA 217

Query: 183 RYAIGRLFHHIPRLESSGAPENSSAEEQKQHLEQFHHARIAGLEPVNFITFATPHLGSRG 242
           RYA+GRLF HIP+L+SSGA ++ S +EQKQH+EQFHH +IAGLEPVNFIT ATPHLGSRG
Sbjct: 218 RYAVGRLFDHIPQLKSSGAAQSFSTDEQKQHIEQFHHEKIAGLEPVNFITVATPHLGSRG 277

Query: 243 NKQFPVLGGLPFLERGASQTAHLVAGRSGKHLFLTDDDDDGKPPLLLRMVTDAEDLKFIS 302
           NKQFPVL GLPFLER ASQTAHLVAGRSGKHLFLTDD++DGKPPLLLRMVTD+EDLKFIS
Sbjct: 278 NKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKFIS 337

Query: 303 ALRSFKRRVAYANVNYDHIL 322
           ALR+FKRRVAYANVNYDH++
Sbjct: 338 ALRAFKRRVAYANVNYDHMV 351

BLAST of MS002270 vs. ExPASy Swiss-Prot
Match: P53118 (Putative lipase ROG1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ROG1 PE=1 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.2e-12
Identity = 68/229 (29.69%), Postives = 99/229 (43.23%), Query Frame = 0

Query: 97  EHLVIMVNGLIGS-AADWRYAAGQFV---KKLPDKVIVHRNECNSSRLTFDGVDTMGERL 156
           +HLV++ +GL  + + D  Y   Q     K  P + IV +    +   T  GV  +G RL
Sbjct: 188 KHLVVLTHGLHSNVSTDLVYIMEQIYKAQKNYPHEQIVVKGYRGNVCQTEKGVKYLGTRL 247

Query: 157 AKEVLAVVRCRPEVQKISFVAHSLGGLVARYAIGRLFHHIPRLESSGAPENSSAEEQKQH 216
           A+ ++  +     ++KISFV HSLGGL+  +AI  ++   P                   
Sbjct: 248 AEYIIQDL-YDESIRKISFVGHSLGGLIQAFAIAYIYEVYPWF----------------- 307

Query: 217 LEQFHHARIAGLEPVNFITFATPHLGSRGNKQFPVLGGLPFLERGASQTAHLVAGRSGKH 276
                      + P+NFIT A+P LG   +    +   L F           V G++G+ 
Sbjct: 308 --------FKKVNPINFITLASPLLGIVTDNPAYIKVLLSF----------GVIGKTGQD 367

Query: 277 LFLTDDDDDGKPPLLLRMVTDAEDLKFISALRSFKRRVAYANVNYDHIL 322
           L L +D + GKP L L        L  I  LR FKRR  YAN   D I+
Sbjct: 368 LGLENDVEVGKPLLYL-----LSGLPLIEILRRFKRRTVYANAINDGIV 375

BLAST of MS002270 vs. ExPASy Swiss-Prot
Match: Q08448 (Lipid droplet phospholipase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=LPL1 PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 2.1e-12
Identity = 62/232 (26.72%), Postives = 104/232 (44.83%), Query Frame = 0

Query: 97  EHLVIMVNGLIGS---AADWRYAAGQFVKK--LPDKVIVHRNECNSSRLTFDGVDTMGER 156
           +HL ++++GL G+       R      +KK  + D +I    + N+   TFDG++ +G R
Sbjct: 5   KHLFVLIHGLWGNYTHMESMRTILSTTLKKEDVNDDMIYFLPKQNAMFKTFDGIEIIGYR 64

Query: 157 LAKEVLAVVRCRPE--VQKISFVAHSLGGLVARYAIGRLFHHIPRLESSGAPENSSAEEQ 216
              EV   +R   +  + K+S + +S GGLVAR+ IG++      L              
Sbjct: 65  TLIEVCEFIRDYKDGKITKLSVMGYSQGGLVARFMIGKMLTEFKEL-------------- 124

Query: 217 KQHLEQFHHARIAGLEPVNFITFATPHLGSRGNKQFPVLGGLPF---LERGASQTAHLVA 276
                         +EP  FIT ATPHLG     +F    G+ +   L          + 
Sbjct: 125 -----------FEDIEPQLFITMATPHLG----VEFYNPTGIAYKSALYSALRTLGSTIL 184

Query: 277 GRSGKHLFLTDDDDDGKPPLLLRMVTDAEDLKFISALRSFKRRVAYANVNYD 319
           G+SG+ +F+ +  ++     +L  ++  E   ++ AL  FK R+A+ANV  D
Sbjct: 185 GKSGREMFIANSSNN-----ILVKLSQGE---YLEALSLFKWRIAFANVKND 199

BLAST of MS002270 vs. ExPASy Swiss-Prot
Match: O14162 (Putative lipase C4A8.10 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC4A8.10 PE=3 SV=2)

HSP 1 Score: 70.5 bits (171), Expect = 4.0e-11
Identity = 60/240 (25.00%), Postives = 103/240 (42.92%), Query Frame = 0

Query: 89  YADAKAAPEHLVIMVNGLIGS-AADWRYAAGQFVK--KLPDKVIVHRNECNSSRLTFDGV 148
           Y+   +   HLV++ +G+  +  AD  Y   + ++  K   +++V R    +   T  GV
Sbjct: 297 YSSFHSKNSHLVVLTHGMHSNVGADMEYLKEKLIESSKSVKELVVVRGFTGNYCQTEKGV 356

Query: 149 DTMGERLAKEVLAVV----RCRPEVQKISFVAHSLGGLVARYAIGRLFHHIPRLESSGAP 208
             +G+RL + +L +        P    IS VAHSLGGLV  YA+G +             
Sbjct: 357 RWLGKRLGEWLLDITGWGSASFPRYSHISVVAHSLGGLVQTYAVGYVH------------ 416

Query: 209 ENSSAEEQKQHLEQFHHARIAGLEPVNFITFATPHLGSRGNKQFPVLGGLPFLERGASQT 268
                        + H A    + PV F+T ATP LG  G     +              
Sbjct: 417 ------------AKTHGAFFQAIHPVFFVTLATPWLGVAGEHPSYI----------GKAL 476

Query: 269 AHLVAGRSGKHLFLTDDDDDGKPPLLLRMVTDAEDLKFISALRSFKRRVAYANVNYDHIL 322
           ++ + G++G+ L LT  +   +    L +++D     F  A+  F++R+ +AN   D+I+
Sbjct: 477 SYGIIGKTGQDLSLTPLNHSIESRPFLVLMSD-PSTPFFQAVSFFEKRILFANTTNDYIV 501

BLAST of MS002270 vs. ExPASy Swiss-Prot
Match: Q12103 (Putative lipase YDL109C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YDL109C PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 3.2e-08
Identity = 62/229 (27.07%), Postives = 95/229 (41.48%), Query Frame = 0

Query: 98  HLVIMVNGLIGS-AADWRYAAGQFVK---KLPDKVIVHRNECNSSRLTFDGVDTMGERLA 157
           HLVI+ +G   + +AD  Y   +  K     P++ +V +    ++  T  G+  +G  LA
Sbjct: 194 HLVILTHGFQSNVSADMEYLMEEIYKAQMNNPNERLVIKGYMKNACETEKGIKFLGVGLA 253

Query: 158 KEVLAVVRCRPEVQKISFVAHSLGGLVARYAIGRLFHHIPRLESSGAPENSSAEEQKQHL 217
             ++  +     V KISF+ HSLGGL   +AI  +    P                    
Sbjct: 254 NYIIDEL-YDDSVGKISFIGHSLGGLTQTFAICYIKTKYPYF------------------ 313

Query: 218 EQFHHARIAGLEPVNFITFATPHLGSRGNKQFPVLGGLP-FLERGASQTAHLVAGRSGKH 277
                     +EP+NFI+ A+P LG        +    P +++   S     + G +G+ 
Sbjct: 314 -------FKKVEPINFISLASPLLG--------IATSTPNYVKMSLSMG---IIGTTGQE 373

Query: 278 LFLTDDDDDGKPPLLLRMVTDAEDLKFISALRSFKRRVAYANVNYDHIL 322
           L L D +   KP L L       +   IS L  FKRR  YAN   D I+
Sbjct: 374 LGLKDGNYGDKPLLYL-----LSEESLISVLARFKRRTLYANAVNDGIV 380

BLAST of MS002270 vs. ExPASy Swiss-Prot
Match: Q9P2D6 (Protein FAM135A OS=Homo sapiens OX=9606 GN=FAM135A PE=1 SV=2)

HSP 1 Score: 60.1 bits (144), Expect = 5.4e-08
Identity = 48/164 (29.27%), Postives = 75/164 (45.73%), Query Frame = 0

Query: 98   HLVIMVNGLIGSAADWRYAAGQFVKKLP----DKVIVHRNECNSSRLTFDGVDTMGERLA 157
            HL++ V+GL G++AD R         LP    D ++  RN+ +    TF   D+M +RL 
Sbjct: 1249 HLIVCVHGLDGNSADLRLVKTYIELGLPGGRIDFLMSERNQND----TFADFDSMTDRLL 1308

Query: 158  KEVLAVVRCRP-EVQKISFVAHSLGGLVARYAIGRLFHHIPRLESSGAPENSSAEEQKQH 217
             E++  ++     V KISF+ HSLG L+ R  + R     PR               K +
Sbjct: 1309 DEIIQYIQIYSLTVSKISFIGHSLGNLIIRSVLTR-----PRF--------------KYY 1368

Query: 218  LEQFHHARIAGLEPVNFITFATPHLGSRGNKQFPVLGGLPFLER 257
            L + H           F++ + PHLG+  N    V  GL F+++
Sbjct: 1369 LNKLH----------TFLSLSGPHLGTLYNSSALVNTGLWFMQK 1379

BLAST of MS002270 vs. ExPASy TrEMBL
Match: A0A6J1C230 (putative lipase YDR444W OS=Momordica charantia OX=3673 GN=LOC111007237 PE=4 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 4.5e-183
Identity = 318/321 (99.07%), Postives = 321/321 (100.00%), Query Frame = 0

Query: 1   MVAANTPLHTPPSGPATAHHPSTTLSRHCSSNPFARFRPKPLPDPAAALLLSHRPKMDLP 60
           MVAANTPLHTPPSGPATAHHPSTTLSRHCSSNPFARFRPKPLPDPAAALLLSHRPKMDLP
Sbjct: 1   MVAANTPLHTPPSGPATAHHPSTTLSRHCSSNPFARFRPKPLPDPAAALLLSHRPKMDLP 60

Query: 61  RRVAAPCFGSRRSKGLKVDPDFGGQEIFYADAKAAPEHLVIMVNGLIGSAADWRYAAGQF 120
           RRVAAPCFGSRRSKGLKVDPDFGGQEIFYADAKAAPEHLVIMVNGLIGSAADWRYAAGQF
Sbjct: 61  RRVAAPCFGSRRSKGLKVDPDFGGQEIFYADAKAAPEHLVIMVNGLIGSAADWRYAAGQF 120

Query: 121 VKKLPDKVIVHRNECNSSRLTFDGVDTMGERLAKEVLAVVRCRPEVQKISFVAHSLGGLV 180
           VKKLPDKVIVHR+ECNSSRLTFDGVDTMGERLAKEVLAVVRCRPEVQKISFVAHSLGGLV
Sbjct: 121 VKKLPDKVIVHRSECNSSRLTFDGVDTMGERLAKEVLAVVRCRPEVQKISFVAHSLGGLV 180

Query: 181 ARYAIGRLFHHIPRLESSGAPENSSAEEQKQHLEQFHHARIAGLEPVNFITFATPHLGSR 240
           ARYAIGRLFHHIPRLESSGAPENSSAEEQKQHLEQFHHARIAGLEPVNFITFATPHLGSR
Sbjct: 181 ARYAIGRLFHHIPRLESSGAPENSSAEEQKQHLEQFHHARIAGLEPVNFITFATPHLGSR 240

Query: 241 GNKQFPVLGGLPFLERGASQTAHLVAGRSGKHLFLTDDDDDGKPPLLLRMVTDAEDLKFI 300
           GNKQFPVLGGLPFLERGASQTAHLVAGRSGKHLFLTDDDDDGKPPLLLRMVTDAEDLKFI
Sbjct: 241 GNKQFPVLGGLPFLERGASQTAHLVAGRSGKHLFLTDDDDDGKPPLLLRMVTDAEDLKFI 300

Query: 301 SALRSFKRRVAYANVNYDHIL 322
           SALRSFKRRVAYANVNYDH++
Sbjct: 301 SALRSFKRRVAYANVNYDHMV 321

BLAST of MS002270 vs. ExPASy TrEMBL
Match: A0A1S3BXX5 (uncharacterized protein LOC103494294 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494294 PE=4 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 2.3e-134
Identity = 251/320 (78.44%), Postives = 274/320 (85.62%), Query Frame = 0

Query: 3   AANTPLHTPPSGPATAHHPSTTLSRHCSSNPFA-RFRPKPLPDPAAALLLSHRPKMDLPR 62
           A  TPLHTPPSGP +  HPSTT   HC SNPF+ RF P P P P     L  RP+MD P 
Sbjct: 38  ATITPLHTPPSGP-SFDHPSTT--PHCPSNPFSLRFPPIPFPFPFPDPSLP-RPRMDFPG 97

Query: 63  RVAAPCFGSRRSKGLKVDPDFGGQEIFYADAKAAPEHLVIMVNGLIGSAADWRYAAGQFV 122
           R+A  CF  R  KGLKVD DF  +E FY DAKA PEHLVIMVNGLIGSAADWRYAAGQFV
Sbjct: 98  RLATGCF--RPRKGLKVDADFTAEEFFYPDAKAPPEHLVIMVNGLIGSAADWRYAAGQFV 157

Query: 123 KKLPDKVIVHRNECNSSRLTFDGVDTMGERLAKEVLAVVRCRPEVQKISFVAHSLGGLVA 182
           KKLPDKVIVHR+ECNSSR TFDGVDTMGERLA+EVL V+R RPE+QKISFVAHSLGGLVA
Sbjct: 158 KKLPDKVIVHRSECNSSRSTFDGVDTMGERLAEEVLGVIRRRPELQKISFVAHSLGGLVA 217

Query: 183 RYAIGRLFHHIPRLESSGAPENSSAEEQKQHLEQFHHARIAGLEPVNFITFATPHLGSRG 242
           RYA+GRLF HIP+L+SSGA ++ S +EQKQH+EQFHH +IAGLEPVNFIT ATPHLGSRG
Sbjct: 218 RYAVGRLFDHIPQLKSSGAAQSFSTDEQKQHIEQFHHEKIAGLEPVNFITVATPHLGSRG 277

Query: 243 NKQFPVLGGLPFLERGASQTAHLVAGRSGKHLFLTDDDDDGKPPLLLRMVTDAEDLKFIS 302
           NKQFPVL GLPFLER ASQTAHLVAGRSGKHLFLTDD++DGKPPLLLRMVTD+EDLKFIS
Sbjct: 278 NKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKFIS 337

Query: 303 ALRSFKRRVAYANVNYDHIL 322
           ALR+FKRRVAYANVNYDH++
Sbjct: 338 ALRAFKRRVAYANVNYDHMV 351

BLAST of MS002270 vs. ExPASy TrEMBL
Match: A0A1S3BWR5 (putative lipase YOR059C isoform X3 OS=Cucumis melo OX=3656 GN=LOC103494294 PE=4 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 2.3e-134
Identity = 251/320 (78.44%), Postives = 274/320 (85.62%), Query Frame = 0

Query: 3   AANTPLHTPPSGPATAHHPSTTLSRHCSSNPFA-RFRPKPLPDPAAALLLSHRPKMDLPR 62
           A  TPLHTPPSGP +  HPSTT   HC SNPF+ RF P P P P     L  RP+MD P 
Sbjct: 38  ATITPLHTPPSGP-SFDHPSTT--PHCPSNPFSLRFPPIPFPFPFPDPSLP-RPRMDFPG 97

Query: 63  RVAAPCFGSRRSKGLKVDPDFGGQEIFYADAKAAPEHLVIMVNGLIGSAADWRYAAGQFV 122
           R+A  CF  R  KGLKVD DF  +E FY DAKA PEHLVIMVNGLIGSAADWRYAAGQFV
Sbjct: 98  RLATGCF--RPRKGLKVDADFTAEEFFYPDAKAPPEHLVIMVNGLIGSAADWRYAAGQFV 157

Query: 123 KKLPDKVIVHRNECNSSRLTFDGVDTMGERLAKEVLAVVRCRPEVQKISFVAHSLGGLVA 182
           KKLPDKVIVHR+ECNSSR TFDGVDTMGERLA+EVL V+R RPE+QKISFVAHSLGGLVA
Sbjct: 158 KKLPDKVIVHRSECNSSRSTFDGVDTMGERLAEEVLGVIRRRPELQKISFVAHSLGGLVA 217

Query: 183 RYAIGRLFHHIPRLESSGAPENSSAEEQKQHLEQFHHARIAGLEPVNFITFATPHLGSRG 242
           RYA+GRLF HIP+L+SSGA ++ S +EQKQH+EQFHH +IAGLEPVNFIT ATPHLGSRG
Sbjct: 218 RYAVGRLFDHIPQLKSSGAAQSFSTDEQKQHIEQFHHEKIAGLEPVNFITVATPHLGSRG 277

Query: 243 NKQFPVLGGLPFLERGASQTAHLVAGRSGKHLFLTDDDDDGKPPLLLRMVTDAEDLKFIS 302
           NKQFPVL GLPFLER ASQTAHLVAGRSGKHLFLTDD++DGKPPLLLRMVTD+EDLKFIS
Sbjct: 278 NKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKFIS 337

Query: 303 ALRSFKRRVAYANVNYDHIL 322
           ALR+FKRRVAYANVNYDH++
Sbjct: 338 ALRAFKRRVAYANVNYDHMV 351

BLAST of MS002270 vs. ExPASy TrEMBL
Match: A0A1S3BWU8 (putative lipase ROG1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494294 PE=4 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 2.3e-134
Identity = 251/320 (78.44%), Postives = 274/320 (85.62%), Query Frame = 0

Query: 3   AANTPLHTPPSGPATAHHPSTTLSRHCSSNPFA-RFRPKPLPDPAAALLLSHRPKMDLPR 62
           A  TPLHTPPSGP +  HPSTT   HC SNPF+ RF P P P P     L  RP+MD P 
Sbjct: 38  ATITPLHTPPSGP-SFDHPSTT--PHCPSNPFSLRFPPIPFPFPFPDPSLP-RPRMDFPG 97

Query: 63  RVAAPCFGSRRSKGLKVDPDFGGQEIFYADAKAAPEHLVIMVNGLIGSAADWRYAAGQFV 122
           R+A  CF  R  KGLKVD DF  +E FY DAKA PEHLVIMVNGLIGSAADWRYAAGQFV
Sbjct: 98  RLATGCF--RPRKGLKVDADFTAEEFFYPDAKAPPEHLVIMVNGLIGSAADWRYAAGQFV 157

Query: 123 KKLPDKVIVHRNECNSSRLTFDGVDTMGERLAKEVLAVVRCRPEVQKISFVAHSLGGLVA 182
           KKLPDKVIVHR+ECNSSR TFDGVDTMGERLA+EVL V+R RPE+QKISFVAHSLGGLVA
Sbjct: 158 KKLPDKVIVHRSECNSSRSTFDGVDTMGERLAEEVLGVIRRRPELQKISFVAHSLGGLVA 217

Query: 183 RYAIGRLFHHIPRLESSGAPENSSAEEQKQHLEQFHHARIAGLEPVNFITFATPHLGSRG 242
           RYA+GRLF HIP+L+SSGA ++ S +EQKQH+EQFHH +IAGLEPVNFIT ATPHLGSRG
Sbjct: 218 RYAVGRLFDHIPQLKSSGAAQSFSTDEQKQHIEQFHHEKIAGLEPVNFITVATPHLGSRG 277

Query: 243 NKQFPVLGGLPFLERGASQTAHLVAGRSGKHLFLTDDDDDGKPPLLLRMVTDAEDLKFIS 302
           NKQFPVL GLPFLER ASQTAHLVAGRSGKHLFLTDD++DGKPPLLLRMVTD+EDLKFIS
Sbjct: 278 NKQFPVLCGLPFLERRASQTAHLVAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDLKFIS 337

Query: 303 ALRSFKRRVAYANVNYDHIL 322
           ALR+FKRRVAYANVNYDH++
Sbjct: 338 ALRAFKRRVAYANVNYDHMV 351

BLAST of MS002270 vs. ExPASy TrEMBL
Match: A0A6J1E5F9 (putative lipase YOR059C isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111430104 PE=4 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 3.9e-134
Identity = 256/324 (79.01%), Postives = 277/324 (85.49%), Query Frame = 0

Query: 3   AANTPLHTPPSGPATAHHPSTTLSRHCSSNPFARFR----PKPLPDPAAALLLSHRPKMD 62
           AANTPL TPPSGP     PS T  RHC SNP  RF     PKP PDP  AL LS+  +MD
Sbjct: 5   AANTPLQTPPSGP--LFDPSAT--RHCLSNPSPRFTIRFPPKPFPDP--ALSLSNHSRMD 64

Query: 63  LPRRVAAPCFGS-RRSKGLKVDPDFGGQEIFYADAKAAPEHLVIMVNGLIGSAADWRYAA 122
           L  R+A   F S RR KGLKV+ DF G+E F  DAKAAPEHLVIMVNGLIGSAADWRYAA
Sbjct: 65  LIGRLANSFFCSLRRLKGLKVEADFSGEETFVPDAKAAPEHLVIMVNGLIGSAADWRYAA 124

Query: 123 GQFVKKLPDKVIVHRNECNSSRLTFDGVDTMGERLAKEVLAVVRCRPEVQKISFVAHSLG 182
            QFV KLPD VIVHR+ECN+SRLTFDGVDTMGERLA+EVLAV+R  PEVQKISFVAHSLG
Sbjct: 125 EQFVMKLPDNVIVHRSECNTSRLTFDGVDTMGERLAEEVLAVIRRWPEVQKISFVAHSLG 184

Query: 183 GLVARYAIGRLFHHIPRLESSGAPENSSAEEQKQHLEQFHHARIAGLEPVNFITFATPHL 242
           GLVARYAIGRLF HIP+LESSGAP++ SAEEQKQ +EQFHHARIAGLEPVNFIT ATPHL
Sbjct: 185 GLVARYAIGRLFDHIPKLESSGAPQSFSAEEQKQQVEQFHHARIAGLEPVNFITVATPHL 244

Query: 243 GSRGNKQFPVLGGLPFLERGASQTAHLVAGRSGKHLFLTDDDDDGKPPLLLRMVTDAEDL 302
           GSRGNKQFPVL GLPFLER ASQTAHL+AGRSGKHLFLTDD++DGKPPLLLRMVTD+EDL
Sbjct: 245 GSRGNKQFPVLCGLPFLERRASQTAHLIAGRSGKHLFLTDDENDGKPPLLLRMVTDSEDL 304

Query: 303 KFISALRSFKRRVAYANVNYDHIL 322
           KFISALR+F+RRVAYANVNYDH++
Sbjct: 305 KFISALRAFERRVAYANVNYDHMV 322

BLAST of MS002270 vs. TAIR 10
Match: AT4G25770.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 323.9 bits (829), Expect = 1.4e-88
Identity = 165/270 (61.11%), Postives = 203/270 (75.19%), Query Frame = 0

Query: 56  KMDLPRRVAAPCFGSRRSK-GLKVDPDFGGQEIFYADAKAA---PEHLVIMVNGLIGSAA 115
           +M+   R+   CF + RSK  L  + D GG++ F AD   +   P+HLV+MVNG++GSAA
Sbjct: 47  QMEWMNRLGIGCFAANRSKRELTTEIDNGGEDFFDADVMESAEKPDHLVVMVNGIVGSAA 106

Query: 116 DWRYAAGQFVKKLPDKVIVHRNECNSSRLTFDGVDTMGERLAKEVLAVVRCRPEVQKISF 175
           DW+YAA QFVKK PDKV+VHR+E NS+ LTFDGVD MGERLA EVL VV+ R  ++KISF
Sbjct: 107 DWKYAAEQFVKKFPDKVLVHRSESNSATLTFDGVDKMGERLANEVLGVVKHRSGLKKISF 166

Query: 176 VAHSLGGLVARYAIGRLFHHIPRLESSGAPENSSAEEQKQHLEQFHHARIAGLEPVNFIT 235
           VAHSLGGLVARYAIG+L+     ++S  +P    +              IAGLEP+NFIT
Sbjct: 167 VAHSLGGLVARYAIGKLYEQPGEVDSLDSPSKEKSA---------RGGEIAGLEPMNFIT 226

Query: 236 FATPHLGSRGNKQFPVLGGLPFLERGASQTAHLVAGRSGKHLFLTDDDDDGKPPLLLRMV 295
           FATPHLGSRG++QFP+L GLPFLER ASQTAHL AGR+GKHLFL  D+DDG  PLL+RM 
Sbjct: 227 FATPHLGSRGHRQFPILCGLPFLERTASQTAHLAAGRTGKHLFLV-DNDDGNAPLLIRMA 286

Query: 296 TDAEDLKFISALRSFKRRVAYANVNYDHIL 322
           TD++DLKFISAL +FKRRVAYANVN+D ++
Sbjct: 287 TDSDDLKFISALNAFKRRVAYANVNFDSMV 306

BLAST of MS002270 vs. TAIR 10
Match: AT4G25770.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 323.9 bits (829), Expect = 1.4e-88
Identity = 165/270 (61.11%), Postives = 203/270 (75.19%), Query Frame = 0

Query: 56  KMDLPRRVAAPCFGSRRSK-GLKVDPDFGGQEIFYADAKAA---PEHLVIMVNGLIGSAA 115
           +M+   R+   CF + RSK  L  + D GG++ F AD   +   P+HLV+MVNG++GSAA
Sbjct: 47  QMEWMNRLGIGCFAANRSKRELTTEIDNGGEDFFDADVMESAEKPDHLVVMVNGIVGSAA 106

Query: 116 DWRYAAGQFVKKLPDKVIVHRNECNSSRLTFDGVDTMGERLAKEVLAVVRCRPEVQKISF 175
           DW+YAA QFVKK PDKV+VHR+E NS+ LTFDGVD MGERLA EVL VV+ R  ++KISF
Sbjct: 107 DWKYAAEQFVKKFPDKVLVHRSESNSATLTFDGVDKMGERLANEVLGVVKHRSGLKKISF 166

Query: 176 VAHSLGGLVARYAIGRLFHHIPRLESSGAPENSSAEEQKQHLEQFHHARIAGLEPVNFIT 235
           VAHSLGGLVARYAIG+L+     ++S  +P    +              IAGLEP+NFIT
Sbjct: 167 VAHSLGGLVARYAIGKLYEQPGEVDSLDSPSKEKSA---------RGGEIAGLEPMNFIT 226

Query: 236 FATPHLGSRGNKQFPVLGGLPFLERGASQTAHLVAGRSGKHLFLTDDDDDGKPPLLLRMV 295
           FATPHLGSRG++QFP+L GLPFLER ASQTAHL AGR+GKHLFL  D+DDG  PLL+RM 
Sbjct: 227 FATPHLGSRGHRQFPILCGLPFLERTASQTAHLAAGRTGKHLFLV-DNDDGNAPLLIRMA 286

Query: 296 TDAEDLKFISALRSFKRRVAYANVNYDHIL 322
           TD++DLKFISAL +FKRRVAYANVN+D ++
Sbjct: 287 TDSDDLKFISALNAFKRRVAYANVNFDSMV 306

BLAST of MS002270 vs. TAIR 10
Match: AT1G10040.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 257.3 bits (656), Expect = 1.6e-68
Identity = 140/252 (55.56%), Postives = 176/252 (69.84%), Query Frame = 0

Query: 70  SRRSKGLKVDPDFGGQEIFYADAKAAPEHLVIMVNGLIGSAADWRYAAGQFVKKLPDKVI 129
           S  S  + +  DF G+       +  P HLV+MVNGLIGSA +WR+AA Q +KK P  ++
Sbjct: 60  SDESGNVDLTVDFPGE-------RTEPTHLVVMVNGLIGSAQNWRFAAKQMLKKYPQDLL 119

Query: 130 VHRNECNSSRLTFDGVDTMGERLAKEVLAVVRCRPEVQKISFVAHSLGGLVARYAIGRLF 189
           VH ++ N S  TFDGVD MGERLA+EV +V++  P +QKISFV HSLGGL+ARYAIGRL+
Sbjct: 120 VHCSKRNHSTQTFDGVDVMGERLAEEVRSVIKRHPSLQKISFVGHSLGGLIARYAIGRLY 179

Query: 190 HHIPRLESSGAPENSSAEEQKQHLEQFHHARIAGLEPVNFITFATPHLGSRGNKQFPVLG 249
               R E    P NS     K  +E+   ARIAGLEPV FIT ATPHLGSRG+KQ P+  
Sbjct: 180 EQESREE---LPHNSDDIGDKCSIEE-PKARIAGLEPVYFITSATPHLGSRGHKQVPLFS 239

Query: 250 GLPFLERGASQTAHLVAGRSGKHLFLTDDDDDGKPPLLLRMVTDAEDLKFISALRSFKRR 309
           G   LER A++ +  + G++GKHLFL  D D GKPPLLLRMV D+ DLKFISAL+ FKRR
Sbjct: 240 GSYTLERLATRMSGCL-GKTGKHLFLA-DSDGGKPPLLLRMVKDSRDLKFISALQCFKRR 298

Query: 310 VAYANVNYDHIL 322
           +AYAN ++DH++
Sbjct: 300 IAYANTSFDHLV 298

BLAST of MS002270 vs. TAIR 10
Match: AT1G10040.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 257.3 bits (656), Expect = 1.6e-68
Identity = 140/252 (55.56%), Postives = 176/252 (69.84%), Query Frame = 0

Query: 70  SRRSKGLKVDPDFGGQEIFYADAKAAPEHLVIMVNGLIGSAADWRYAAGQFVKKLPDKVI 129
           S  S  + +  DF G+       +  P HLV+MVNGLIGSA +WR+AA Q +KK P  ++
Sbjct: 60  SDESGNVDLTVDFPGE-------RTEPTHLVVMVNGLIGSAQNWRFAAKQMLKKYPQDLL 119

Query: 130 VHRNECNSSRLTFDGVDTMGERLAKEVLAVVRCRPEVQKISFVAHSLGGLVARYAIGRLF 189
           VH ++ N S  TFDGVD MGERLA+EV +V++  P +QKISFV HSLGGL+ARYAIGRL+
Sbjct: 120 VHCSKRNHSTQTFDGVDVMGERLAEEVRSVIKRHPSLQKISFVGHSLGGLIARYAIGRLY 179

Query: 190 HHIPRLESSGAPENSSAEEQKQHLEQFHHARIAGLEPVNFITFATPHLGSRGNKQFPVLG 249
               R E    P NS     K  +E+   ARIAGLEPV FIT ATPHLGSRG+KQ P+  
Sbjct: 180 EQESREE---LPHNSDDIGDKCSIEE-PKARIAGLEPVYFITSATPHLGSRGHKQVPLFS 239

Query: 250 GLPFLERGASQTAHLVAGRSGKHLFLTDDDDDGKPPLLLRMVTDAEDLKFISALRSFKRR 309
           G   LER A++ +  + G++GKHLFL  D D GKPPLLLRMV D+ DLKFISAL+ FKRR
Sbjct: 240 GSYTLERLATRMSGCL-GKTGKHLFLA-DSDGGKPPLLLRMVKDSRDLKFISALQCFKRR 298

Query: 310 VAYANVNYDHIL 322
           +AYAN ++DH++
Sbjct: 300 IAYANTSFDHLV 298

BLAST of MS002270 vs. TAIR 10
Match: AT5G51180.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 246.5 bits (628), Expect = 2.9e-65
Identity = 124/228 (54.39%), Postives = 161/228 (70.61%), Query Frame = 0

Query: 94  AAPEHLVIMVNGLIGSAADWRYAAGQFVKKLPDKVIVHRNECNSSRLTFDGVDTMGERLA 153
           ++ +HLV+MV+G++GS  DW++ A QFVKK+PDKV VH +E N S LT DGVD MGERLA
Sbjct: 31  SSADHLVVMVHGILGSTDDWKFGAEQFVKKMPDKVFVHCSEKNVSALTLDGVDVMGERLA 90

Query: 154 KEVLAVVRCRPEVQKISFVAHSLGGLVARYAIGRLFHHIPRLESSGAPENSSAEEQKQHL 213
            EVL +++ +P + KISFVAHSLGGL ARYAIG+L+    + +   +  +SS E  K   
Sbjct: 91  AEVLDIIQRKPNICKISFVAHSLGGLAARYAIGKLYKPANQEDVKDSVADSSQETPK--- 150

Query: 214 EQFHHARIAGLEPVNFITFATPHLGSRGNKQFPVLGGLPFLERGASQTAHLVAGRSGKHL 273
                  I GLE +NFIT ATPHLGS GNKQ P L G   +E+ A    H +  R+G+HL
Sbjct: 151 -----GTICGLEAMNFITVATPHLGSMGNKQVPFLFGFSSIEKVAGLIIHWIFKRTGRHL 210

Query: 274 FLTDDDDDGKPPLLLRMVTDAEDLKFISALRSFKRRVAYANVNYDHIL 322
           FL  D+++GKPPLL RMV D +D  FISALR+FKRRVAY+NV +DH++
Sbjct: 211 FL-KDEEEGKPPLLRRMVEDTDDCHFISALRAFKRRVAYSNVGHDHVV 249