MS002258 (gene) Bitter gourd (TR) v1

Overview
NameMS002258
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionXyloglucan endotransglucosylase/hydrolase
Locationscaffold30: 3661552 .. 3662587 (+)
RNA-Seq ExpressionMS002258
SyntenyMS002258
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TATTTCTCACCAGTTGGTTGTGTCTCATTACAAATGGCTCCCACAACTCTACTGCTGACCCTTCTCTCTGCCTCCCTAATTGCCCTCTCCGCAGCCAACTTCAACCACGACTTTCACATCACTTGGGGAGATGGCCGGGCCAACATTCTCGACAATGGGCAGCTCCTCACCCTCTCCCTCGACAAGGCCTCCGGCTCCGGCTTCCAGTCCCAGAACCACTACTTATTTGGAAACATTGATATGCAACTCAAGCTCGTGCCTGGCAACTCCGCCGGCACTGTCACTGCCTATTATGTAAAGCCTAAGCCTAATTTTTTGTTGCTAAATTAGCTTAATTTTGATTGGTTTTTTTGGCAGTTGTCTTCCAAAGGGTCGAATTGGGACGAGATAGACTTCGAGTTCTTGGGGAATCTGAGCGGTGACCCCTACACTCTCCACACCAATGTGTTTAGCCAAGGCAGAGGCAACAGAGAGCAACAATTTCATCTCTGGTTCGACCCCACCGCTGATTTCCACACCTATTCCATTCTCTGGAACCCCAACCGCATTATGTAATTCACACCTGACGCACCCATCTTACAAACTTTCTTTATTCATGCTTACAATTTCAGACACAAAATTGGAACTTTAAGAATACCCATATTTCAGATTTTCTGTGGACGGAACTCCAATAAGGGAATTCAAGAACTGGGAGTCGAAAGGGGTGGCATTTCCGAAGAGGCAGGCGATGAGGATATACTCGAGCTTGTGGAACGCAGAGGATTGGGCGACCAGAGGAGGGGCGGTGAAGACGGACTGGGCGGCGGCTCCTTTCACGGCGTCTTACAGGAACTTCAGGGCCGAGGCGTGCGTTTGGGCGTCGGGGCGCTCCTCTTGCGGCCCCACTGGGAATCCCAGAGCAAACTCCTCGTGGCTGTCCCAAGAGTTGGACTCTGCGAGTGAAGAAAAAATGAAGTGGGTGCAGAAGAATTACATGATTTATAATTATTGTGCGGATACTAAGAGGTTTCCACAGGGCCTCCCTACGGAATGTAAA

mRNA sequence

TATTTCTCACCAGTTGGTTGTGTCTCATTACAAATGGCTCCCACAACTCTACTGCTGACCCTTCTCTCTGCCTCCCTAATTGCCCTCTCCGCAGCCAACTTCAACCACGACTTTCACATCACTTGGGGAGATGGCCGGGCCAACATTCTCGACAATGGGCAGCTCCTCACCCTCTCCCTCGACAAGGCCTCCGGCTCCGGCTTCCAGTCCCAGAACCACTACTTATTTGGAAACATTGATATGCAACTCAAGCTCGTGCCTGGCAACTCCGCCGGCACTGTCACTGCCTATTATTTGTCTTCCAAAGGGTCGAATTGGGACGAGATAGACTTCGAGTTCTTGGGGAATCTGAGCGGTGACCCCTACACTCTCCACACCAATGTGTTTAGCCAAGGCAGAGGCAACAGAGAGCAACAATTTCATCTCTGGTTCGACCCCACCGCTGATTTCCACACCTATTCCATTCTCTGGAACCCCAACCGCATTATATTTTCTGTGGACGGAACTCCAATAAGGGAATTCAAGAACTGGGAGTCGAAAGGGGTGGCATTTCCGAAGAGGCAGGCGATGAGGATATACTCGAGCTTGTGGAACGCAGAGGATTGGGCGACCAGAGGAGGGGCGGTGAAGACGGACTGGGCGGCGGCTCCTTTCACGGCGTCTTACAGGAACTTCAGGGCCGAGGCGTGCGTTTGGGCGTCGGGGCGCTCCTCTTGCGGCCCCACTGGGAATCCCAGAGCAAACTCCTCGTGGCTGTCCCAAGAGTTGGACTCTGCGAGTGAAGAAAAAATGAAGTGGGTGCAGAAGAATTACATGATTTATAATTATTGTGCGGATACTAAGAGGTTTCCACAGGGCCTCCCTACGGAATGTAAA

Coding sequence (CDS)

TATTTCTCACCAGTTGGTTGTGTCTCATTACAAATGGCTCCCACAACTCTACTGCTGACCCTTCTCTCTGCCTCCCTAATTGCCCTCTCCGCAGCCAACTTCAACCACGACTTTCACATCACTTGGGGAGATGGCCGGGCCAACATTCTCGACAATGGGCAGCTCCTCACCCTCTCCCTCGACAAGGCCTCCGGCTCCGGCTTCCAGTCCCAGAACCACTACTTATTTGGAAACATTGATATGCAACTCAAGCTCGTGCCTGGCAACTCCGCCGGCACTGTCACTGCCTATTATTTGTCTTCCAAAGGGTCGAATTGGGACGAGATAGACTTCGAGTTCTTGGGGAATCTGAGCGGTGACCCCTACACTCTCCACACCAATGTGTTTAGCCAAGGCAGAGGCAACAGAGAGCAACAATTTCATCTCTGGTTCGACCCCACCGCTGATTTCCACACCTATTCCATTCTCTGGAACCCCAACCGCATTATATTTTCTGTGGACGGAACTCCAATAAGGGAATTCAAGAACTGGGAGTCGAAAGGGGTGGCATTTCCGAAGAGGCAGGCGATGAGGATATACTCGAGCTTGTGGAACGCAGAGGATTGGGCGACCAGAGGAGGGGCGGTGAAGACGGACTGGGCGGCGGCTCCTTTCACGGCGTCTTACAGGAACTTCAGGGCCGAGGCGTGCGTTTGGGCGTCGGGGCGCTCCTCTTGCGGCCCCACTGGGAATCCCAGAGCAAACTCCTCGTGGCTGTCCCAAGAGTTGGACTCTGCGAGTGAAGAAAAAATGAAGTGGGTGCAGAAGAATTACATGATTTATAATTATTGTGCGGATACTAAGAGGTTTCCACAGGGCCTCCCTACGGAATGTAAA

Protein sequence

YFSPVGCVSLQMAPTTLLLTLLSASLIALSAANFNHDFHITWGDGRANILDNGQLLTLSLDKASGSGFQSQNHYLFGNIDMQLKLVPGNSAGTVTAYYLSSKGSNWDEIDFEFLGNLSGDPYTLHTNVFSQGRGNREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESKGVAFPKRQAMRIYSSLWNAEDWATRGGAVKTDWAAAPFTASYRNFRAEACVWASGRSSCGPTGNPRANSSWLSQELDSASEEKMKWVQKNYMIYNYCADTKRFPQGLPTECK
Homology
BLAST of MS002258 vs. NCBI nr
Match: XP_038878721.1 (probable xyloglucan endotransglucosylase/hydrolase protein 23 [Benincasa hispida])

HSP 1 Score: 508.1 bits (1307), Expect = 5.2e-140
Identity = 241/281 (85.77%), Postives = 256/281 (91.10%), Query Frame = 0

Query: 12  MAPTTLLLTLL-SASLIALSAANFNHDFHITWGDGRANILDNGQLLTLSLDKASGSGFQS 71
           MA TTL LTLL SA L+ALS+ANFN DF ITWGDGRANIL+NGQLLTLSLDKASGSGFQS
Sbjct: 1   MASTTLFLTLLFSAYLVALSSANFNQDFQITWGDGRANILNNGQLLTLSLDKASGSGFQS 60

Query: 72  QNHYLFGNIDMQLKLVPGNSAGTVTAYYLSSKGSNWDEIDFEFLGNLSGDPYTLHTNVFS 131
           QN YLFG IDMQLKLV GNSAGTVTAYYLSSKGS WDEIDFEFLGNLSGDPYTLHTNVFS
Sbjct: 61  QNEYLFGKIDMQLKLVAGNSAGTVTAYYLSSKGSTWDEIDFEFLGNLSGDPYTLHTNVFS 120

Query: 132 QGRGNREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESKGVAFPKRQAM 191
           QG+GNREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWES GV FPK Q M
Sbjct: 121 QGKGNREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESNGVPFPKNQPM 180

Query: 192 RIYSSLWNAEDWATRGGAVKTDWAAAPFTASYRNFRAEACVWASGRSSCGPTGNPRANSS 251
           RIYSSLWNA+DWATRGG VKTDW  APFTASYRNF+AEACVW++G SSC P+    + +S
Sbjct: 181 RIYSSLWNADDWATRGGVVKTDWTKAPFTASYRNFKAEACVWSAGGSSCSPSSG--SGNS 240

Query: 252 WLSQELDSASEEKMKWVQKNYMIYNYCADTKRFPQGLPTEC 292
           WLSQE+DSAS+E++KWVQKNYMIYNYC DTKRFPQGLPTEC
Sbjct: 241 WLSQEMDSASQERLKWVQKNYMIYNYCTDTKRFPQGLPTEC 279

BLAST of MS002258 vs. NCBI nr
Match: NP_001267702.1 (probable xyloglucan endotransglucosylase/hydrolase protein 23-like precursor [Cucumis sativus] >CAD87533.1 putative xyloglucan endotransglycosylase [Cucumis sativus] >CAD87535.1 putative xyloglucan endotransglycosylase [Cucumis sativus])

HSP 1 Score: 505.4 bits (1300), Expect = 3.4e-139
Identity = 240/281 (85.41%), Postives = 256/281 (91.10%), Query Frame = 0

Query: 12  MAPTTLLLTLL-SASLIALSAANFNHDFHITWGDGRANILDNGQLLTLSLDKASGSGFQS 71
           MA TTL LTLL SASLIA+S+ANFN DF ITWGDGRA IL+NG+LLTLSLDKASGSGFQS
Sbjct: 1   MASTTLFLTLLFSASLIAISSANFNQDFQITWGDGRAKILNNGELLTLSLDKASGSGFQS 60

Query: 72  QNHYLFGNIDMQLKLVPGNSAGTVTAYYLSSKGSNWDEIDFEFLGNLSGDPYTLHTNVFS 131
           QN YLFG IDMQLKLV GNSAGTVTAYYLSSKGS WDEIDFEFLGNLSGDPYTLHTNVFS
Sbjct: 61  QNEYLFGKIDMQLKLVAGNSAGTVTAYYLSSKGSTWDEIDFEFLGNLSGDPYTLHTNVFS 120

Query: 132 QGRGNREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESKGVAFPKRQAM 191
           QG+GNREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWES GV FPK Q M
Sbjct: 121 QGKGNREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESNGVPFPKDQPM 180

Query: 192 RIYSSLWNAEDWATRGGAVKTDWAAAPFTASYRNFRAEACVWASGRSSCGPTGNPRANSS 251
           RIYSSLWNA+DWATRGG VKTDW  APFTASY+NF+AEACVW+ G+SSC P+    +N S
Sbjct: 181 RIYSSLWNADDWATRGGLVKTDWTKAPFTASYKNFKAEACVWSGGKSSCSPSPAGSSN-S 240

Query: 252 WLSQELDSASEEKMKWVQKNYMIYNYCADTKRFPQGLPTEC 292
           WLSQE+DSAS+E++KWVQKNYMIYNYC DTKRFPQGLPTEC
Sbjct: 241 WLSQEMDSASQERLKWVQKNYMIYNYCTDTKRFPQGLPTEC 280

BLAST of MS002258 vs. NCBI nr
Match: KAE8653160.1 (hypothetical protein Csa_019870 [Cucumis sativus])

HSP 1 Score: 505.4 bits (1300), Expect = 3.4e-139
Identity = 240/281 (85.41%), Postives = 256/281 (91.10%), Query Frame = 0

Query: 12  MAPTTLLLTLL-SASLIALSAANFNHDFHITWGDGRANILDNGQLLTLSLDKASGSGFQS 71
           MA TTL LTLL SASLIA+S+ANFN DF ITWGDGRA IL+NG+LLTLSLDKASGSGFQS
Sbjct: 37  MASTTLFLTLLFSASLIAISSANFNQDFQITWGDGRAKILNNGELLTLSLDKASGSGFQS 96

Query: 72  QNHYLFGNIDMQLKLVPGNSAGTVTAYYLSSKGSNWDEIDFEFLGNLSGDPYTLHTNVFS 131
           QN YLFG IDMQLKLV GNSAGTVTAYYLSSKGS WDEIDFEFLGNLSGDPYTLHTNVFS
Sbjct: 97  QNEYLFGKIDMQLKLVAGNSAGTVTAYYLSSKGSTWDEIDFEFLGNLSGDPYTLHTNVFS 156

Query: 132 QGRGNREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESKGVAFPKRQAM 191
           QG+GNREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWES GV FPK Q M
Sbjct: 157 QGKGNREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESNGVPFPKDQPM 216

Query: 192 RIYSSLWNAEDWATRGGAVKTDWAAAPFTASYRNFRAEACVWASGRSSCGPTGNPRANSS 251
           RIYSSLWNA+DWATRGG VKTDW  APFTASY+NF+AEACVW+ G+SSC P+    +N S
Sbjct: 217 RIYSSLWNADDWATRGGLVKTDWTKAPFTASYKNFKAEACVWSGGKSSCSPSPAGSSN-S 276

Query: 252 WLSQELDSASEEKMKWVQKNYMIYNYCADTKRFPQGLPTEC 292
           WLSQE+DSAS+E++KWVQKNYMIYNYC DTKRFPQGLPTEC
Sbjct: 277 WLSQEMDSASQERLKWVQKNYMIYNYCTDTKRFPQGLPTEC 316

BLAST of MS002258 vs. NCBI nr
Match: XP_022987545.1 (probable xyloglucan endotransglucosylase/hydrolase protein 23 [Cucurbita maxima])

HSP 1 Score: 504.6 bits (1298), Expect = 5.7e-139
Identity = 242/287 (84.32%), Postives = 256/287 (89.20%), Query Frame = 0

Query: 11  QMAPTTLLLTLL--SASLIALSAANFNHDFHITWGDGRANILDNGQLLTLSLDKASGSGF 70
           QMA TTLLL  L  SASLIALS+ANFN DF ITWGDGRA I++NG+LLTLSLDKASGSGF
Sbjct: 12  QMASTTLLLITLFVSASLIALSSANFNQDFQITWGDGRAKIVNNGELLTLSLDKASGSGF 71

Query: 71  QSQNHYLFGNIDMQLKLVPGNSAGTVTAYYLSSKGSNWDEIDFEFLGNLSGDPYTLHTNV 130
           QSQN YLFG IDMQLKLV GNSAGTVTAYYLSSKGS WDEIDFEFLGNLSGDPYTLHTNV
Sbjct: 72  QSQNEYLFGKIDMQLKLVAGNSAGTVTAYYLSSKGSTWDEIDFEFLGNLSGDPYTLHTNV 131

Query: 131 FSQGRGNREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESKGVAFPKRQ 190
           FSQG+GNREQQFHLWFDPTADFHTYSILWNP RIIFSVDGTPIREFKNWES GV FPK Q
Sbjct: 132 FSQGKGNREQQFHLWFDPTADFHTYSILWNPTRIIFSVDGTPIREFKNWESNGVPFPKSQ 191

Query: 191 AMRIYSSLWNAEDWATRGGAVKTDWAAAPFTASYRNFRAEACVWASGRSSCGP----TGN 250
            MRIYSSLWNA+DWATRGG VKTDW  APFTASYRNF+AEACVW+SG SSC      +GN
Sbjct: 192 PMRIYSSLWNADDWATRGGLVKTDWTKAPFTASYRNFKAEACVWSSGGSSCSSPPSGSGN 251

Query: 251 PRANSSWLSQELDSASEEKMKWVQKNYMIYNYCADTKRFPQGLPTEC 292
             A +SWLSQE+DSAS+E++KWVQKNYMIYNYC DTKRFPQGLPTEC
Sbjct: 252 AMAKNSWLSQEMDSASQERLKWVQKNYMIYNYCTDTKRFPQGLPTEC 298

BLAST of MS002258 vs. NCBI nr
Match: XP_023515612.1 (probable xyloglucan endotransglucosylase/hydrolase protein 23 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 503.1 bits (1294), Expect = 1.7e-138
Identity = 241/286 (84.27%), Postives = 256/286 (89.51%), Query Frame = 0

Query: 12  MAPTTLLLTLL--SASLIALSAANFNHDFHITWGDGRANILDNGQLLTLSLDKASGSGFQ 71
           MA TTLLL  L  SASL+ALS+ANFN DF ITWGDGRA I++NG+LLTLSLDKASGSGFQ
Sbjct: 1   MASTTLLLVTLFVSASLVALSSANFNQDFQITWGDGRAKIVNNGELLTLSLDKASGSGFQ 60

Query: 72  SQNHYLFGNIDMQLKLVPGNSAGTVTAYYLSSKGSNWDEIDFEFLGNLSGDPYTLHTNVF 131
           SQN YLFG IDMQLKLV GNSAGTVTAYYLSSKGS WDEIDFEFLGNLSGDPYTLHTNVF
Sbjct: 61  SQNEYLFGKIDMQLKLVAGNSAGTVTAYYLSSKGSTWDEIDFEFLGNLSGDPYTLHTNVF 120

Query: 132 SQGRGNREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESKGVAFPKRQA 191
           SQG+GNREQQFHLWFDPTADFHTYSILWNP RIIFSVDGTPIREFKNWES GV FPK Q 
Sbjct: 121 SQGKGNREQQFHLWFDPTADFHTYSILWNPTRIIFSVDGTPIREFKNWESNGVPFPKSQP 180

Query: 192 MRIYSSLWNAEDWATRGGAVKTDWAAAPFTASYRNFRAEACVWASGRSSCGP----TGNP 251
           MRIYSSLWNA+DWATRGG VKTDW  APFTASYRNF+AEACVW+SG SSC      +GN 
Sbjct: 181 MRIYSSLWNADDWATRGGLVKTDWTKAPFTASYRNFKAEACVWSSGGSSCSSPPSGSGNA 240

Query: 252 RANSSWLSQELDSASEEKMKWVQKNYMIYNYCADTKRFPQGLPTEC 292
            A +SWLSQE+DSAS+E++KWVQKNYMIYNYCADTKRFPQGLPTEC
Sbjct: 241 IAKNSWLSQEMDSASQERLKWVQKNYMIYNYCADTKRFPQGLPTEC 286

BLAST of MS002258 vs. ExPASy Swiss-Prot
Match: Q38910 (Probable xyloglucan endotransglucosylase/hydrolase protein 23 OS=Arabidopsis thaliana OX=3702 GN=XTH23 PE=2 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 2.3e-122
Identity = 204/277 (73.65%), Postives = 229/277 (82.67%), Query Frame = 0

Query: 15  TTLLLTLLSASLIALSAANFNHDFHITWGDGRANILDNGQLLTLSLDKASGSGFQSQNHY 74
           +T+++ LL++ +I   +ANF  D  ITWGDGR  I +NG LLTLSLDKASGSGFQS+N Y
Sbjct: 7   STIVVALLASFMICSVSANFQRDVEITWGDGRGQITNNGDLLTLSLDKASGSGFQSKNEY 66

Query: 75  LFGNIDMQLKLVPGNSAGTVTAYYLSSKGSNWDEIDFEFLGNLSGDPYTLHTNVFSQGRG 134
           LFG IDMQ+KLV GNSAGTVTAYYL S GS WDEIDFEFLGNLSGDPYTLHTNVF+QG+G
Sbjct: 67  LFGKIDMQIKLVAGNSAGTVTAYYLKSPGSTWDEIDFEFLGNLSGDPYTLHTNVFTQGKG 126

Query: 135 NREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESKGVAFPKRQAMRIYS 194
           +REQQF LWFDPT+DFHTYSILWNP RIIFSVDGTPIREFKN ES+G  FPK Q MR+YS
Sbjct: 127 DREQQFKLWFDPTSDFHTYSILWNPQRIIFSVDGTPIREFKNMESQGTLFPKNQPMRMYS 186

Query: 195 SLWNAEDWATRGGAVKTDWAAAPFTASYRNFRAEACVWASGRSSCGPTGNPRANSSWLSQ 254
           SLWNAE+WATRGG VKTDW+ APFTASYR F  EACV  +G+SSC       +  SWLSQ
Sbjct: 187 SLWNAEEWATRGGLVKTDWSKAPFTASYRGFNEEACVVINGQSSCPNVSGQGSTGSWLSQ 246

Query: 255 ELDSASEEKMKWVQKNYMIYNYCADTKRFPQGLPTEC 292
           ELDS  +E+M+WVQ NYMIYNYC D KRFPQGLP EC
Sbjct: 247 ELDSTGQEQMRWVQNNYMIYNYCTDAKRFPQGLPREC 283

BLAST of MS002258 vs. ExPASy Swiss-Prot
Match: Q38857 (Xyloglucan endotransglucosylase/hydrolase protein 22 OS=Arabidopsis thaliana OX=3702 GN=XTH22 PE=1 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 1.3e-117
Identity = 196/282 (69.50%), Postives = 232/282 (82.27%), Query Frame = 0

Query: 12  MAPTTLLLTLLSASLIALSAANFNHDFHITWGDGRANILDNGQLLTLSLDKASGSGFQSQ 71
           MA T LL   LS  + +  +ANF  D  ITWGDGR  I +NG+LLTLSLDK+SGSGFQS+
Sbjct: 1   MAITYLLPLFLSLIITSSVSANFQRDVEITWGDGRGQIKNNGELLTLSLDKSSGSGFQSK 60

Query: 72  NHYLFGNIDMQLKLVPGNSAGTVTAYYLSSKGSNWDEIDFEFLGNLSGDPYTLHTNVFSQ 131
           N YLFG + MQ+KLVPGNSAGTVT  YL S G+ WDEIDFEFLGN SG+PYTLHTNV++Q
Sbjct: 61  NEYLFGKVSMQMKLVPGNSAGTVTTLYLKSPGTTWDEIDFEFLGNSSGEPYTLHTNVYTQ 120

Query: 132 GRGNREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESKGVAFPKRQAMR 191
           G+G++EQQF LWFDPTA+FHTY+ILWNP RIIF+VDGTPIREFKN ES G  FPK + MR
Sbjct: 121 GKGDKEQQFKLWFDPTANFHTYTILWNPQRIIFTVDGTPIREFKNMESLGTLFPKNKPMR 180

Query: 192 IYSSLWNAEDWATRGGAVKTDWAAAPFTASYRNFRAEACVWASGRSSCGPTGNPRANS-- 251
           +YSSLWNA+DWATRGG VKTDW+ APFTASYR F+ EACVW++G+SSC P  + +  +  
Sbjct: 181 MYSSLWNADDWATRGGLVKTDWSKAPFTASYRGFQQEACVWSNGKSSC-PNASKQGTTTG 240

Query: 252 SWLSQELDSASEEKMKWVQKNYMIYNYCADTKRFPQGLPTEC 292
           SWLSQELDS ++++M+WVQ+NYMIYNYC D KRFPQGLP EC
Sbjct: 241 SWLSQELDSTAQQRMRWVQRNYMIYNYCTDAKRFPQGLPKEC 281

BLAST of MS002258 vs. ExPASy Swiss-Prot
Match: Q38907 (Probable xyloglucan endotransglucosylase/hydrolase protein 25 OS=Arabidopsis thaliana OX=3702 GN=XTH25 PE=2 SV=2)

HSP 1 Score: 417.5 bits (1072), Expect = 1.2e-115
Identity = 193/280 (68.93%), Postives = 228/280 (81.43%), Query Frame = 0

Query: 16  TLLLTLLSASLIALS---AANFNHDFHITWGDGRANILDNGQLLTLSLDKASGSGFQSQN 75
           +LL TL  ++    S   A  F+ +F ITWGDGR  +L+NG+LLTLSLD+ASGSGFQ++ 
Sbjct: 9   SLLFTLTVSTTTLFSPVFAGTFDTEFDITWGDGRGKVLNNGELLTLSLDRASGSGFQTKK 68

Query: 76  HYLFGNIDMQLKLVPGNSAGTVTAYYLSSKGSNWDEIDFEFLGNLSGDPYTLHTNVFSQG 135
            YLFG IDMQLKLVPGNSAGTVTAYYL SKG  WDEIDFEFLGNL+GDPYT+HTNV++QG
Sbjct: 69  EYLFGKIDMQLKLVPGNSAGTVTAYYLKSKGDTWDEIDFEFLGNLTGDPYTMHTNVYTQG 128

Query: 136 RGNREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESKGVAFPKRQAMRI 195
           +G+REQQFHLWFDPTADFHTYS+LWNP+ I+F VD  P+REFKN +  G+ +PK Q MR+
Sbjct: 129 KGDREQQFHLWFDPTADFHTYSVLWNPHHIVFMVDDIPVREFKNLQHMGIQYPKLQPMRL 188

Query: 196 YSSLWNAEDWATRGGAVKTDWAAAPFTASYRNFRAEACVWASGRSSCGPTGNPRANSSWL 255
           YSSLWNA+ WATRGG VKTDW+ APFTASYRNFRA+ACV + GRSSC P G+PR    W 
Sbjct: 189 YSSLWNADQWATRGGLVKTDWSKAPFTASYRNFRADACVSSGGRSSC-PAGSPR----WF 248

Query: 256 SQELDSASEEKMKWVQKNYMIYNYCADTKRFPQGLPTECK 293
           SQ LD  +E+KM+ VQ+ YMIYNYC DTKRFPQG P EC+
Sbjct: 249 SQRLDLTAEDKMRVVQRKYMIYNYCTDTKRFPQGFPKECR 283

BLAST of MS002258 vs. ExPASy Swiss-Prot
Match: P24806 (Xyloglucan endotransglucosylase/hydrolase protein 24 OS=Arabidopsis thaliana OX=3702 GN=XTH24 PE=1 SV=2)

HSP 1 Score: 401.7 bits (1031), Expect = 6.9e-111
Identity = 182/272 (66.91%), Postives = 220/272 (80.88%), Query Frame = 0

Query: 20  TLLSASLIALSAANFNHDFHITWGDGRANILDNGQLLTLSLDKASGSGFQSQNHYLFGNI 79
           T L  +  ++SAA+FN D ++ WG+GR  IL+NGQLLTLSLDK+SGSGFQS+  YLFG I
Sbjct: 10  TTLLVAAFSVSAADFNTDVNVAWGNGRGKILNNGQLLTLSLDKSSGSGFQSKTEYLFGKI 69

Query: 80  DMQLKLVPGNSAGTVTAYYLSSKGSNWDEIDFEFLGNLSGDPYTLHTNVFSQGRGNREQQ 139
           DMQ+KLVPGNSAGTVT +YL S+GS WDEIDFEFLGN+SGDPYTLHTNV++QG+G++EQQ
Sbjct: 70  DMQIKLVPGNSAGTVTTFYLKSEGSTWDEIDFEFLGNMSGDPYTLHTNVYTQGKGDKEQQ 129

Query: 140 FHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESKGVAFPKRQAMRIYSSLWNA 199
           FHLWFDPTA+FHTYSILWNP RII +VD TPIREFKN+ES GV FPK + MR+Y+SLWNA
Sbjct: 130 FHLWFDPTANFHTYSILWNPQRIILTVDDTPIREFKNYESLGVLFPKNKPMRMYASLWNA 189

Query: 200 EDWATRGGAVKTDWAAAPFTASYRNFRAEACVWASGRSSCGPTGNPRANSSWLSQELDSA 259
           +DWATRGG VKTDW+ APF ASYRN + ++                + NS+W +QE+DS 
Sbjct: 190 DDWATRGGLVKTDWSKAPFMASYRNIKIDS----------------KPNSNWYTQEMDST 249

Query: 260 SEEKMKWVQKNYMIYNYCADTKRFPQGLPTEC 292
           S+ ++KWVQKNYMIYNYC D +RFPQG P EC
Sbjct: 250 SQARLKWVQKNYMIYNYCTDHRRFPQGAPKEC 265

BLAST of MS002258 vs. ExPASy Swiss-Prot
Match: Q9FI31 (Xyloglucan endotransglucosylase/hydrolase protein 20 OS=Arabidopsis thaliana OX=3702 GN=XTH20 PE=2 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 6.6e-106
Identity = 173/276 (62.68%), Postives = 213/276 (77.17%), Query Frame = 0

Query: 17  LLLTLLSASLIALSAANFNHDFHITWGDGRANILDN-GQLLTLSLDKASGSGFQSQNHYL 76
           L++ L +A    + A +F+ D  I WGDGR  ILDN G LL+LSLDK SGSGFQS   +L
Sbjct: 12  LIIFLFAAQYERVYAGSFHKDVQIHWGDGRGKILDNVGNLLSLSLDKFSGSGFQSHQEFL 71

Query: 77  FGNIDMQLKLVPGNSAGTVTAYYLSSKGSNWDEIDFEFLGNLSGDPYTLHTNVFSQGRGN 136
           +G +++Q+KLVPGNSAGTVT +YL S G+ WDEIDFEFLGN+SG PYTLHTNV+++G G+
Sbjct: 72  YGKVEVQMKLVPGNSAGTVTTFYLKSPGTTWDEIDFEFLGNISGHPYTLHTNVYTKGTGD 131

Query: 137 REQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESKGVAFPKRQAMRIYSS 196
           +EQQFHLWFDPT DFHTY I+WNP R+IF++DG PIREFKN E+ GV FPK Q MR+Y+S
Sbjct: 132 KEQQFHLWFDPTVDFHTYCIIWNPQRVIFTIDGIPIREFKNSEALGVPFPKHQPMRLYAS 191

Query: 197 LWNAEDWATRGGAVKTDWAAAPFTASYRNFRAEACVWASGRSSCGPTGNPRANSSWLSQE 256
           LW AE WATRGG  KTDW+ APFTA YRN+  +ACVW++G+SSC       ANSSW +Q 
Sbjct: 192 LWEAEHWATRGGLEKTDWSKAPFTAFYRNYNVDACVWSNGKSSCS------ANSSWFTQV 251

Query: 257 LDSASEEKMKWVQKNYMIYNYCADTKRFPQGLPTEC 292
           LD   + ++KW Q+ YM+YNYC D KRFPQG P EC
Sbjct: 252 LDFKGKNRVKWAQRKYMVYNYCTDKKRFPQGAPPEC 281

BLAST of MS002258 vs. ExPASy TrEMBL
Match: Q84JX3 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=XTH1 PE=2 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 1.6e-139
Identity = 240/281 (85.41%), Postives = 256/281 (91.10%), Query Frame = 0

Query: 12  MAPTTLLLTLL-SASLIALSAANFNHDFHITWGDGRANILDNGQLLTLSLDKASGSGFQS 71
           MA TTL LTLL SASLIA+S+ANFN DF ITWGDGRA IL+NG+LLTLSLDKASGSGFQS
Sbjct: 1   MASTTLFLTLLFSASLIAISSANFNQDFQITWGDGRAKILNNGELLTLSLDKASGSGFQS 60

Query: 72  QNHYLFGNIDMQLKLVPGNSAGTVTAYYLSSKGSNWDEIDFEFLGNLSGDPYTLHTNVFS 131
           QN YLFG IDMQLKLV GNSAGTVTAYYLSSKGS WDEIDFEFLGNLSGDPYTLHTNVFS
Sbjct: 61  QNEYLFGKIDMQLKLVAGNSAGTVTAYYLSSKGSTWDEIDFEFLGNLSGDPYTLHTNVFS 120

Query: 132 QGRGNREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESKGVAFPKRQAM 191
           QG+GNREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWES GV FPK Q M
Sbjct: 121 QGKGNREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESNGVPFPKDQPM 180

Query: 192 RIYSSLWNAEDWATRGGAVKTDWAAAPFTASYRNFRAEACVWASGRSSCGPTGNPRANSS 251
           RIYSSLWNA+DWATRGG VKTDW  APFTASY+NF+AEACVW+ G+SSC P+    +N S
Sbjct: 181 RIYSSLWNADDWATRGGLVKTDWTKAPFTASYKNFKAEACVWSGGKSSCSPSPAGSSN-S 240

Query: 252 WLSQELDSASEEKMKWVQKNYMIYNYCADTKRFPQGLPTEC 292
           WLSQE+DSAS+E++KWVQKNYMIYNYC DTKRFPQGLPTEC
Sbjct: 241 WLSQEMDSASQERLKWVQKNYMIYNYCTDTKRFPQGLPTEC 280

BLAST of MS002258 vs. ExPASy TrEMBL
Match: A0A6J1JH63 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111485078 PE=3 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 2.8e-139
Identity = 242/287 (84.32%), Postives = 256/287 (89.20%), Query Frame = 0

Query: 11  QMAPTTLLLTLL--SASLIALSAANFNHDFHITWGDGRANILDNGQLLTLSLDKASGSGF 70
           QMA TTLLL  L  SASLIALS+ANFN DF ITWGDGRA I++NG+LLTLSLDKASGSGF
Sbjct: 12  QMASTTLLLITLFVSASLIALSSANFNQDFQITWGDGRAKIVNNGELLTLSLDKASGSGF 71

Query: 71  QSQNHYLFGNIDMQLKLVPGNSAGTVTAYYLSSKGSNWDEIDFEFLGNLSGDPYTLHTNV 130
           QSQN YLFG IDMQLKLV GNSAGTVTAYYLSSKGS WDEIDFEFLGNLSGDPYTLHTNV
Sbjct: 72  QSQNEYLFGKIDMQLKLVAGNSAGTVTAYYLSSKGSTWDEIDFEFLGNLSGDPYTLHTNV 131

Query: 131 FSQGRGNREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESKGVAFPKRQ 190
           FSQG+GNREQQFHLWFDPTADFHTYSILWNP RIIFSVDGTPIREFKNWES GV FPK Q
Sbjct: 132 FSQGKGNREQQFHLWFDPTADFHTYSILWNPTRIIFSVDGTPIREFKNWESNGVPFPKSQ 191

Query: 191 AMRIYSSLWNAEDWATRGGAVKTDWAAAPFTASYRNFRAEACVWASGRSSCGP----TGN 250
            MRIYSSLWNA+DWATRGG VKTDW  APFTASYRNF+AEACVW+SG SSC      +GN
Sbjct: 192 PMRIYSSLWNADDWATRGGLVKTDWTKAPFTASYRNFKAEACVWSSGGSSCSSPPSGSGN 251

Query: 251 PRANSSWLSQELDSASEEKMKWVQKNYMIYNYCADTKRFPQGLPTEC 292
             A +SWLSQE+DSAS+E++KWVQKNYMIYNYC DTKRFPQGLPTEC
Sbjct: 252 AMAKNSWLSQEMDSASQERLKWVQKNYMIYNYCTDTKRFPQGLPTEC 298

BLAST of MS002258 vs. ExPASy TrEMBL
Match: A0A6J1E169 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111429655 PE=3 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 2.3e-138
Identity = 240/286 (83.92%), Postives = 255/286 (89.16%), Query Frame = 0

Query: 12  MAPTTLLLTLL--SASLIALSAANFNHDFHITWGDGRANILDNGQLLTLSLDKASGSGFQ 71
           MA TTLLL  L  SASL+ALS+ANFN DF ITWGDGRA I++NG+LLTLSLDKASGSGFQ
Sbjct: 1   MASTTLLLVTLFVSASLVALSSANFNQDFQITWGDGRAKIVNNGELLTLSLDKASGSGFQ 60

Query: 72  SQNHYLFGNIDMQLKLVPGNSAGTVTAYYLSSKGSNWDEIDFEFLGNLSGDPYTLHTNVF 131
           SQN YLFG IDMQLKLV GNSAGTVTAYYLSSKGS WDEIDFEFLGNLSGDPYTLHTNVF
Sbjct: 61  SQNEYLFGKIDMQLKLVAGNSAGTVTAYYLSSKGSTWDEIDFEFLGNLSGDPYTLHTNVF 120

Query: 132 SQGRGNREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESKGVAFPKRQA 191
           SQG+GNREQQFHLWFDPTADFHTYSILWNP RIIFSVDGTPIREFKNWES GV FPK Q 
Sbjct: 121 SQGKGNREQQFHLWFDPTADFHTYSILWNPTRIIFSVDGTPIREFKNWESNGVPFPKTQP 180

Query: 192 MRIYSSLWNAEDWATRGGAVKTDWAAAPFTASYRNFRAEACVWASGRSSCGP----TGNP 251
           MRIYSSLWNA+DWATRGG VKTDW  APFTASYRNF+AEACVW+SG SSC      +GN 
Sbjct: 181 MRIYSSLWNADDWATRGGLVKTDWTKAPFTASYRNFKAEACVWSSGGSSCSSPPSGSGNA 240

Query: 252 RANSSWLSQELDSASEEKMKWVQKNYMIYNYCADTKRFPQGLPTEC 292
            A +SWLSQE+DSAS+E++KWVQKNYMIYNYC DTKRFPQGLPTEC
Sbjct: 241 IAKNSWLSQEMDSASQERLKWVQKNYMIYNYCTDTKRFPQGLPTEC 286

BLAST of MS002258 vs. ExPASy TrEMBL
Match: Q06BI4 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo OX=3656 GN=XTH3 PE=2 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 1.1e-135
Identity = 230/283 (81.27%), Postives = 253/283 (89.40%), Query Frame = 0

Query: 12  MAPTTLLLTLL--SASLIALSAANFNHDFHITWGDGRANILDNGQLLTLSLDKASGSGFQ 71
           MA   + LTLL  +ASLIA+S+ANFN DF ITWGDGRA IL+NG+LLTLSLDKASGSGFQ
Sbjct: 1   MASPIMFLTLLFSAASLIAISSANFNQDFQITWGDGRAKILNNGELLTLSLDKASGSGFQ 60

Query: 72  SQNHYLFGNIDMQLKLVPGNSAGTVTAYYLSSKGSNWDEIDFEFLGNLSGDPYTLHTNVF 131
           SQN YLFG IDMQLKLV GNSAGTVTAYYLSSKGS WDEIDFEFLGNL+GDPYTLHTNVF
Sbjct: 61  SQNEYLFGKIDMQLKLVAGNSAGTVTAYYLSSKGSTWDEIDFEFLGNLTGDPYTLHTNVF 120

Query: 132 SQGRGNREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESKGVAFPKRQA 191
           SQG+GNREQQFHLWFDPTADFHTYSILWNPNRI+FSVDGTPIREFKNWES GV FPK Q 
Sbjct: 121 SQGKGNREQQFHLWFDPTADFHTYSILWNPNRIVFSVDGTPIREFKNWESNGVPFPKDQP 180

Query: 192 MRIYSSLWNAEDWATRGGAVKTDWAAAPFTASYRNFRAEACVWASGRSSCGPTGNPRANS 251
           MRIYSSLWNA+DWATRGG VKTDW  APFTASY+NF+AEACV + G+SSC P+    + +
Sbjct: 181 MRIYSSLWNADDWATRGGLVKTDWTKAPFTASYKNFKAEACVLSGGQSSCSPSAPAGSRN 240

Query: 252 SWLSQELDSASEEKMKWVQKNYMIYNYCADTKRFPQGLPTECK 293
           SWLS+E+DS S+E++KWVQKNYMIYNYC DT+RFPQGLPTECK
Sbjct: 241 SWLSEEMDSVSQERLKWVQKNYMIYNYCTDTRRFPQGLPTECK 283

BLAST of MS002258 vs. ExPASy TrEMBL
Match: A0A7N2R0A6 (Xyloglucan endotransglucosylase/hydrolase OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 4.7e-131
Identity = 225/278 (80.94%), Postives = 244/278 (87.77%), Query Frame = 0

Query: 17  LLLTLLSASLIALSAANFNHDFHITWGDGRANILDNGQLLTLSLDKASGSGFQSQNHYLF 76
           +LL LL  S++  SAAN N DF ITWGDGRANI++NG+LLTLSLDK SGSGFQS+N YLF
Sbjct: 12  ILLVLLLGSIMVASAANLNQDFDITWGDGRANIINNGELLTLSLDKTSGSGFQSKNEYLF 71

Query: 77  GNIDMQLKLVPGNSAGTVTAYYLSSKGSNWDEIDFEFLGNLSGDPYTLHTNVFSQGRGNR 136
           G IDMQLKLVPGNSAGTVTAYYLSSKGS WDEIDFEFLGNLSGDPY LHTNVFSQG+GNR
Sbjct: 72  GKIDMQLKLVPGNSAGTVTAYYLSSKGSTWDEIDFEFLGNLSGDPYILHTNVFSQGKGNR 131

Query: 137 EQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESKGVAFPKRQAMRIYSSL 196
           EQQF+LWFDPTADFHTYSILWNP RIIFSVDGTPIREFKN ES GV FPK Q MRIYSSL
Sbjct: 132 EQQFYLWFDPTADFHTYSILWNPQRIIFSVDGTPIREFKNLESNGVPFPKNQPMRIYSSL 191

Query: 197 WNAEDWATRGGAVKTDWAAAPFTASYRNFRAEACVWASGRSSCGPTGNPRA---NSSWLS 256
           WNA+DWATRGG VKTDW  APFTASYRNF A ACVW SG SSCG + +P A   N++WLS
Sbjct: 192 WNADDWATRGGLVKTDWTQAPFTASYRNFNANACVWTSGASSCG-SNSPSATSSNNAWLS 251

Query: 257 QELDSASEEKMKWVQKNYMIYNYCADTKRFPQGLPTEC 292
           QELDSAS+++++W QKNYMIYNYC DTKRFPQGLPTEC
Sbjct: 252 QELDSASQQRLQWAQKNYMIYNYCTDTKRFPQGLPTEC 288

BLAST of MS002258 vs. TAIR 10
Match: AT4G25810.1 (xyloglucan endotransglycosylase 6 )

HSP 1 Score: 439.9 bits (1130), Expect = 1.6e-123
Identity = 204/277 (73.65%), Postives = 229/277 (82.67%), Query Frame = 0

Query: 15  TTLLLTLLSASLIALSAANFNHDFHITWGDGRANILDNGQLLTLSLDKASGSGFQSQNHY 74
           +T+++ LL++ +I   +ANF  D  ITWGDGR  I +NG LLTLSLDKASGSGFQS+N Y
Sbjct: 7   STIVVALLASFMICSVSANFQRDVEITWGDGRGQITNNGDLLTLSLDKASGSGFQSKNEY 66

Query: 75  LFGNIDMQLKLVPGNSAGTVTAYYLSSKGSNWDEIDFEFLGNLSGDPYTLHTNVFSQGRG 134
           LFG IDMQ+KLV GNSAGTVTAYYL S GS WDEIDFEFLGNLSGDPYTLHTNVF+QG+G
Sbjct: 67  LFGKIDMQIKLVAGNSAGTVTAYYLKSPGSTWDEIDFEFLGNLSGDPYTLHTNVFTQGKG 126

Query: 135 NREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESKGVAFPKRQAMRIYS 194
           +REQQF LWFDPT+DFHTYSILWNP RIIFSVDGTPIREFKN ES+G  FPK Q MR+YS
Sbjct: 127 DREQQFKLWFDPTSDFHTYSILWNPQRIIFSVDGTPIREFKNMESQGTLFPKNQPMRMYS 186

Query: 195 SLWNAEDWATRGGAVKTDWAAAPFTASYRNFRAEACVWASGRSSCGPTGNPRANSSWLSQ 254
           SLWNAE+WATRGG VKTDW+ APFTASYR F  EACV  +G+SSC       +  SWLSQ
Sbjct: 187 SLWNAEEWATRGGLVKTDWSKAPFTASYRGFNEEACVVINGQSSCPNVSGQGSTGSWLSQ 246

Query: 255 ELDSASEEKMKWVQKNYMIYNYCADTKRFPQGLPTEC 292
           ELDS  +E+M+WVQ NYMIYNYC D KRFPQGLP EC
Sbjct: 247 ELDSTGQEQMRWVQNNYMIYNYCTDAKRFPQGLPREC 283

BLAST of MS002258 vs. TAIR 10
Match: AT5G57560.1 (Xyloglucan endotransglucosylase/hydrolase family protein )

HSP 1 Score: 424.1 bits (1089), Expect = 9.2e-119
Identity = 196/282 (69.50%), Postives = 232/282 (82.27%), Query Frame = 0

Query: 12  MAPTTLLLTLLSASLIALSAANFNHDFHITWGDGRANILDNGQLLTLSLDKASGSGFQSQ 71
           MA T LL   LS  + +  +ANF  D  ITWGDGR  I +NG+LLTLSLDK+SGSGFQS+
Sbjct: 1   MAITYLLPLFLSLIITSSVSANFQRDVEITWGDGRGQIKNNGELLTLSLDKSSGSGFQSK 60

Query: 72  NHYLFGNIDMQLKLVPGNSAGTVTAYYLSSKGSNWDEIDFEFLGNLSGDPYTLHTNVFSQ 131
           N YLFG + MQ+KLVPGNSAGTVT  YL S G+ WDEIDFEFLGN SG+PYTLHTNV++Q
Sbjct: 61  NEYLFGKVSMQMKLVPGNSAGTVTTLYLKSPGTTWDEIDFEFLGNSSGEPYTLHTNVYTQ 120

Query: 132 GRGNREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESKGVAFPKRQAMR 191
           G+G++EQQF LWFDPTA+FHTY+ILWNP RIIF+VDGTPIREFKN ES G  FPK + MR
Sbjct: 121 GKGDKEQQFKLWFDPTANFHTYTILWNPQRIIFTVDGTPIREFKNMESLGTLFPKNKPMR 180

Query: 192 IYSSLWNAEDWATRGGAVKTDWAAAPFTASYRNFRAEACVWASGRSSCGPTGNPRANS-- 251
           +YSSLWNA+DWATRGG VKTDW+ APFTASYR F+ EACVW++G+SSC P  + +  +  
Sbjct: 181 MYSSLWNADDWATRGGLVKTDWSKAPFTASYRGFQQEACVWSNGKSSC-PNASKQGTTTG 240

Query: 252 SWLSQELDSASEEKMKWVQKNYMIYNYCADTKRFPQGLPTEC 292
           SWLSQELDS ++++M+WVQ+NYMIYNYC D KRFPQGLP EC
Sbjct: 241 SWLSQELDSTAQQRMRWVQRNYMIYNYCTDAKRFPQGLPKEC 281

BLAST of MS002258 vs. TAIR 10
Match: AT5G57550.1 (xyloglucan endotransglucosylase/hydrolase 25 )

HSP 1 Score: 417.5 bits (1072), Expect = 8.6e-117
Identity = 193/280 (68.93%), Postives = 228/280 (81.43%), Query Frame = 0

Query: 16  TLLLTLLSASLIALS---AANFNHDFHITWGDGRANILDNGQLLTLSLDKASGSGFQSQN 75
           +LL TL  ++    S   A  F+ +F ITWGDGR  +L+NG+LLTLSLD+ASGSGFQ++ 
Sbjct: 9   SLLFTLTVSTTTLFSPVFAGTFDTEFDITWGDGRGKVLNNGELLTLSLDRASGSGFQTKK 68

Query: 76  HYLFGNIDMQLKLVPGNSAGTVTAYYLSSKGSNWDEIDFEFLGNLSGDPYTLHTNVFSQG 135
            YLFG IDMQLKLVPGNSAGTVTAYYL SKG  WDEIDFEFLGNL+GDPYT+HTNV++QG
Sbjct: 69  EYLFGKIDMQLKLVPGNSAGTVTAYYLKSKGDTWDEIDFEFLGNLTGDPYTMHTNVYTQG 128

Query: 136 RGNREQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESKGVAFPKRQAMRI 195
           +G+REQQFHLWFDPTADFHTYS+LWNP+ I+F VD  P+REFKN +  G+ +PK Q MR+
Sbjct: 129 KGDREQQFHLWFDPTADFHTYSVLWNPHHIVFMVDDIPVREFKNLQHMGIQYPKLQPMRL 188

Query: 196 YSSLWNAEDWATRGGAVKTDWAAAPFTASYRNFRAEACVWASGRSSCGPTGNPRANSSWL 255
           YSSLWNA+ WATRGG VKTDW+ APFTASYRNFRA+ACV + GRSSC P G+PR    W 
Sbjct: 189 YSSLWNADQWATRGGLVKTDWSKAPFTASYRNFRADACVSSGGRSSC-PAGSPR----WF 248

Query: 256 SQELDSASEEKMKWVQKNYMIYNYCADTKRFPQGLPTECK 293
           SQ LD  +E+KM+ VQ+ YMIYNYC DTKRFPQG P EC+
Sbjct: 249 SQRLDLTAEDKMRVVQRKYMIYNYCTDTKRFPQGFPKECR 283

BLAST of MS002258 vs. TAIR 10
Match: AT4G30270.1 (xyloglucan endotransglucosylase/hydrolase 24 )

HSP 1 Score: 401.7 bits (1031), Expect = 4.9e-112
Identity = 182/272 (66.91%), Postives = 220/272 (80.88%), Query Frame = 0

Query: 20  TLLSASLIALSAANFNHDFHITWGDGRANILDNGQLLTLSLDKASGSGFQSQNHYLFGNI 79
           T L  +  ++SAA+FN D ++ WG+GR  IL+NGQLLTLSLDK+SGSGFQS+  YLFG I
Sbjct: 10  TTLLVAAFSVSAADFNTDVNVAWGNGRGKILNNGQLLTLSLDKSSGSGFQSKTEYLFGKI 69

Query: 80  DMQLKLVPGNSAGTVTAYYLSSKGSNWDEIDFEFLGNLSGDPYTLHTNVFSQGRGNREQQ 139
           DMQ+KLVPGNSAGTVT +YL S+GS WDEIDFEFLGN+SGDPYTLHTNV++QG+G++EQQ
Sbjct: 70  DMQIKLVPGNSAGTVTTFYLKSEGSTWDEIDFEFLGNMSGDPYTLHTNVYTQGKGDKEQQ 129

Query: 140 FHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESKGVAFPKRQAMRIYSSLWNA 199
           FHLWFDPTA+FHTYSILWNP RII +VD TPIREFKN+ES GV FPK + MR+Y+SLWNA
Sbjct: 130 FHLWFDPTANFHTYSILWNPQRIILTVDDTPIREFKNYESLGVLFPKNKPMRMYASLWNA 189

Query: 200 EDWATRGGAVKTDWAAAPFTASYRNFRAEACVWASGRSSCGPTGNPRANSSWLSQELDSA 259
           +DWATRGG VKTDW+ APF ASYRN + ++                + NS+W +QE+DS 
Sbjct: 190 DDWATRGGLVKTDWSKAPFMASYRNIKIDS----------------KPNSNWYTQEMDST 249

Query: 260 SEEKMKWVQKNYMIYNYCADTKRFPQGLPTEC 292
           S+ ++KWVQKNYMIYNYC D +RFPQG P EC
Sbjct: 250 SQARLKWVQKNYMIYNYCTDHRRFPQGAPKEC 265

BLAST of MS002258 vs. TAIR 10
Match: AT5G48070.1 (xyloglucan endotransglucosylase/hydrolase 20 )

HSP 1 Score: 385.2 bits (988), Expect = 4.7e-107
Identity = 173/276 (62.68%), Postives = 213/276 (77.17%), Query Frame = 0

Query: 17  LLLTLLSASLIALSAANFNHDFHITWGDGRANILDN-GQLLTLSLDKASGSGFQSQNHYL 76
           L++ L +A    + A +F+ D  I WGDGR  ILDN G LL+LSLDK SGSGFQS   +L
Sbjct: 12  LIIFLFAAQYERVYAGSFHKDVQIHWGDGRGKILDNVGNLLSLSLDKFSGSGFQSHQEFL 71

Query: 77  FGNIDMQLKLVPGNSAGTVTAYYLSSKGSNWDEIDFEFLGNLSGDPYTLHTNVFSQGRGN 136
           +G +++Q+KLVPGNSAGTVT +YL S G+ WDEIDFEFLGN+SG PYTLHTNV+++G G+
Sbjct: 72  YGKVEVQMKLVPGNSAGTVTTFYLKSPGTTWDEIDFEFLGNISGHPYTLHTNVYTKGTGD 131

Query: 137 REQQFHLWFDPTADFHTYSILWNPNRIIFSVDGTPIREFKNWESKGVAFPKRQAMRIYSS 196
           +EQQFHLWFDPT DFHTY I+WNP R+IF++DG PIREFKN E+ GV FPK Q MR+Y+S
Sbjct: 132 KEQQFHLWFDPTVDFHTYCIIWNPQRVIFTIDGIPIREFKNSEALGVPFPKHQPMRLYAS 191

Query: 197 LWNAEDWATRGGAVKTDWAAAPFTASYRNFRAEACVWASGRSSCGPTGNPRANSSWLSQE 256
           LW AE WATRGG  KTDW+ APFTA YRN+  +ACVW++G+SSC       ANSSW +Q 
Sbjct: 192 LWEAEHWATRGGLEKTDWSKAPFTAFYRNYNVDACVWSNGKSSCS------ANSSWFTQV 251

Query: 257 LDSASEEKMKWVQKNYMIYNYCADTKRFPQGLPTEC 292
           LD   + ++KW Q+ YM+YNYC D KRFPQG P EC
Sbjct: 252 LDFKGKNRVKWAQRKYMVYNYCTDKKRFPQGAPPEC 281

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038878721.15.2e-14085.77probable xyloglucan endotransglucosylase/hydrolase protein 23 [Benincasa hispida... [more]
NP_001267702.13.4e-13985.41probable xyloglucan endotransglucosylase/hydrolase protein 23-like precursor [Cu... [more]
KAE8653160.13.4e-13985.41hypothetical protein Csa_019870 [Cucumis sativus][more]
XP_022987545.15.7e-13984.32probable xyloglucan endotransglucosylase/hydrolase protein 23 [Cucurbita maxima][more]
XP_023515612.11.7e-13884.27probable xyloglucan endotransglucosylase/hydrolase protein 23 [Cucurbita pepo su... [more]
Match NameE-valueIdentityDescription
Q389102.3e-12273.65Probable xyloglucan endotransglucosylase/hydrolase protein 23 OS=Arabidopsis tha... [more]
Q388571.3e-11769.50Xyloglucan endotransglucosylase/hydrolase protein 22 OS=Arabidopsis thaliana OX=... [more]
Q389071.2e-11568.93Probable xyloglucan endotransglucosylase/hydrolase protein 25 OS=Arabidopsis tha... [more]
P248066.9e-11166.91Xyloglucan endotransglucosylase/hydrolase protein 24 OS=Arabidopsis thaliana OX=... [more]
Q9FI316.6e-10662.68Xyloglucan endotransglucosylase/hydrolase protein 20 OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
Q84JX31.6e-13985.41Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=XTH1 PE=... [more]
A0A6J1JH632.8e-13984.32Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1E1692.3e-13883.92Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Q06BI41.1e-13581.27Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo OX=3656 GN=XTH3 PE=2 S... [more]
A0A7N2R0A64.7e-13180.94Xyloglucan endotransglucosylase/hydrolase OS=Quercus lobata OX=97700 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G25810.11.6e-12373.65xyloglucan endotransglycosylase 6 [more]
AT5G57560.19.2e-11969.50Xyloglucan endotransglucosylase/hydrolase family protein [more]
AT5G57550.18.6e-11768.93xyloglucan endotransglucosylase/hydrolase 25 [more]
AT4G30270.14.9e-11266.91xyloglucan endotransglucosylase/hydrolase 24 [more]
AT5G48070.14.7e-10762.68xyloglucan endotransglucosylase/hydrolase 20 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010713Xyloglucan endo-transglycosylase, C-terminalPFAMPF06955XET_Ccoord: 248..291
e-value: 7.9E-22
score: 76.8
IPR000757Glycoside hydrolase family 16PFAMPF00722Glyco_hydro_16coord: 35..214
e-value: 3.4E-60
score: 202.6
IPR000757Glycoside hydrolase family 16PROSITEPS51762GH16_2coord: 1..222
score: 29.436298
NoneNo IPR availableGENE3D2.60.120.200coord: 20..292
e-value: 8.8E-110
score: 368.3
NoneNo IPR availablePANTHERPTHR31062:SF178XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASEcoord: 17..292
IPR016455Xyloglucan endotransglucosylase/hydrolasePIRSFPIRSF005604EndGlu_transfcoord: 3..292
e-value: 9.0E-118
score: 390.6
IPR016455Xyloglucan endotransglucosylase/hydrolaseCDDcd02176GH16_XETcoord: 30..291
e-value: 3.53823E-154
score: 429.312
IPR044791Beta-glucanase/XTHPANTHERPTHR31062XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATEDcoord: 17..292
IPR008263Glycoside hydrolase, family 16, active sitePROSITEPS01034GH16_1coord: 108..118
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 34..291

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002258.1MS002258.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006073 cellular glucan metabolic process
biological_process GO:0042546 cell wall biogenesis
biological_process GO:0010411 xyloglucan metabolic process
cellular_component GO:0048046 apoplast
cellular_component GO:0005618 cell wall
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0016762 xyloglucan:xyloglucosyl transferase activity