MS002251 (gene) Bitter gourd (TR) v1

BLAST of MS002251 vs. NCBI nr
Match: KAG6587759.1 (4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 852.4 bits (2201), Expect = 2.1e-243
Identity = 445/549 (81.06%), Postives = 486/549 (88.52%), Query Frame = 0

Query: 1   MAAADPPPEFIFRSKLPDITIPNHLPLHTYCFEKLPEFRDRPCLINAATGETHTYGEVHK 60
           MAAADP PEFIFRSKLPDITIP+HLPLHTYCFE + +F+ RPCLIN ATG+T+TY EVH 
Sbjct: 1   MAAADPSPEFIFRSKLPDITIPDHLPLHTYCFENISQFKHRPCLINGATGDTYTYDEVHV 60

Query: 61  TARRVAAGLHRMGIRKGDVIMLLLQNSPQFVLAFLGASFLGAVATMANPFFTATEVAKQA 120
           TARRVAAGLH++G+ KGDVIMLLLQNSPQFVLAFLGAS++GAVATMANPFFT  E+AK A
Sbjct: 61  TARRVAAGLHKLGVGKGDVIMLLLQNSPQFVLAFLGASYIGAVATMANPFFTPPEIAKHA 120

Query: 121 GSAGAKLIITQAAFAAKVKNLTCHENDVIIKVIYIDQPPPDDDGGRHFSELM-RAAEEEI 180
            S+GAKLIITQAAFA KVK+L   +N V IK+I+ID PP    G  HFS L     EEE+
Sbjct: 121 ASSGAKLIITQAAFAEKVKSLV-DKNGVSIKLIFIDPPP---YGEVHFSVLTDDVKEEEM 180

Query: 181 PEVKISPDDVVALPYSSGTTGLPKGVMLTHRALVTSVAQQVDGENPHLNVRRDDVVLCVL 240
           P+VKISP+DVVALPYSSGTTGLPKGVMLTHR LVTSVAQQVDGENPHL +R DDVVLCVL
Sbjct: 181 PDVKISPNDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPHLYIRSDDVVLCVL 240

Query: 241 PLFHIYSLNCIMMCSLRVGAAILIVHKFDINSLVKFVPKYKVTVAPLVPPIVLAIAESPA 300
           PLFHIYSLN IMMCSLRVGAAILIV KFDIN +V+ V KYKVT+APLVPPIVLAIA+SPA
Sbjct: 241 PLFHIYSLNSIMMCSLRVGAAILIVQKFDINYVVELVGKYKVTIAPLVPPIVLAIAKSPA 300

Query: 301 VESSDMSSIRMVLSGAAPLGKELEDAVKAKLPQAILGQGYRMTE-GLVFTMSLAFAKERF 360
           V  ++MSSIRMVLSGAAPLGKELEDA +AKLP AILGQGY MTE G   T+SLAFAKERF
Sbjct: 301 VGGTEMSSIRMVLSGAAPLGKELEDAFRAKLPDAILGQGYGMTEAGSALTLSLAFAKERF 360

Query: 361 EVKSGACGTLMRNSEMKIINPQTGVSLPRNQPGEICIRSTQFMKGYLHDEEATKGIIDKN 420
            +KSGACGTLMRNS+MKI+NPQTG SLPRNQPGEI IRS   MKGYL++EEATK IID++
Sbjct: 361 PIKSGACGTLMRNSDMKIVNPQTGASLPRNQPGEIWIRSPHVMKGYLNNEEATKAIIDEH 420

Query: 421 GWLHSGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVVPMKDE 480
           GWLH+GDIGFVD+DDEVFIVDRLKELIKYKGFQVAPAELEALLISH HIADAAV+PMK+E
Sbjct: 421 GWLHTGDIGFVDEDDEVFIVDRLKELIKYKGFQVAPAELEALLISHTHIADAAVIPMKNE 480

Query: 481 VAGEVPAAFIVRSDSSNIAEDEIKQYISKQVVFYKRINRVFFVDSIPKSSSGKILRRQLR 540
           VAGEVP AFIVRS  SNI EDEIKQYISKQVVFYKRI+RVFFVDSIPKS SGKILRRQLR
Sbjct: 481 VAGEVPVAFIVRSGGSNITEDEIKQYISKQVVFYKRIHRVFFVDSIPKSPSGKILRRQLR 540

Query: 541 ALLAASIPN 548
            LLAA IPN
Sbjct: 541 ELLAARIPN 545

BLAST of MS002251 vs. NCBI nr
Match: XP_023531498.1 (4-coumarate--CoA ligase 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 852.0 bits (2200), Expect = 2.7e-243
Identity = 443/549 (80.69%), Postives = 485/549 (88.34%), Query Frame = 0

Query: 1   MAAADPPPEFIFRSKLPDITIPNHLPLHTYCFEKLPEFRDRPCLINAATGETHTYGEVHK 60
           MAAADP PEFIFRSKLPDITIP+HLPLHTYCFE + +F+ RPC+IN ATGET+TY EVH 
Sbjct: 1   MAAADPSPEFIFRSKLPDITIPDHLPLHTYCFENISQFKHRPCIINGATGETYTYDEVHV 60

Query: 61  TARRVAAGLHRMGIRKGDVIMLLLQNSPQFVLAFLGASFLGAVATMANPFFTATEVAKQA 120
           TARRVAAGLH++G+ KGDVIMLLLQNSPQFVLAFLGAS++GAVATMANPFFT  E+AK  
Sbjct: 61  TARRVAAGLHKLGVGKGDVIMLLLQNSPQFVLAFLGASYIGAVATMANPFFTPPEIAKHV 120

Query: 121 GSAGAKLIITQAAFAAKVKNLTCHENDVIIKVIYIDQPPPDDDGGRHFSELM-RAAEEEI 180
            S+GAKLIITQA FA KVK+L   +N V IK+I+ID PP   DG  HFS L     EE++
Sbjct: 121 ASSGAKLIITQAVFAEKVKSLV-DKNGVSIKLIFIDPPP---DGEVHFSVLTDDVKEEDM 180

Query: 181 PEVKISPDDVVALPYSSGTTGLPKGVMLTHRALVTSVAQQVDGENPHLNVRRDDVVLCVL 240
           P+VKISP+DVVALPYSSGTTGLPKGVMLTHR LVTSVAQQVDGENPHL +R DDVVLCVL
Sbjct: 181 PDVKISPNDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPHLYIRSDDVVLCVL 240

Query: 241 PLFHIYSLNCIMMCSLRVGAAILIVHKFDINSLVKFVPKYKVTVAPLVPPIVLAIAESPA 300
           PLFHIYSLN IMMCSLRVGAAILIV KFDIN +V+ V KYKVT+APLVPPIVLAIA+SPA
Sbjct: 241 PLFHIYSLNSIMMCSLRVGAAILIVQKFDINYVVELVGKYKVTIAPLVPPIVLAIAKSPA 300

Query: 301 VESSDMSSIRMVLSGAAPLGKELEDAVKAKLPQAILGQGYRMTE-GLVFTMSLAFAKERF 360
           V  ++MSSIRMVLSGAAPLGKELEDA +AKLP AILGQGY MTE G   T+SLAFAKERF
Sbjct: 301 VGGTEMSSIRMVLSGAAPLGKELEDAFRAKLPDAILGQGYGMTEAGSALTLSLAFAKERF 360

Query: 361 EVKSGACGTLMRNSEMKIINPQTGVSLPRNQPGEICIRSTQFMKGYLHDEEATKGIIDKN 420
            +KSGACGTLMRNS+MKI+NPQTG SLPRNQPGEI IRS   MKGYL++EEATK IID++
Sbjct: 361 PIKSGACGTLMRNSDMKIVNPQTGASLPRNQPGEIWIRSPHVMKGYLNNEEATKAIIDEH 420

Query: 421 GWLHSGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVVPMKDE 480
           GWLH+GDIGFVD+DDEVFIVDRLKELIKYKGFQVAPAELEALLISH HIADAAV+PMK+E
Sbjct: 421 GWLHTGDIGFVDEDDEVFIVDRLKELIKYKGFQVAPAELEALLISHTHIADAAVIPMKNE 480

Query: 481 VAGEVPAAFIVRSDSSNIAEDEIKQYISKQVVFYKRINRVFFVDSIPKSSSGKILRRQLR 540
           VAGEVP AFIVRS  SNI EDEIKQYISKQVVFYKRI+RVFFVDSIPKS SGKILRRQLR
Sbjct: 481 VAGEVPVAFIVRSGGSNITEDEIKQYISKQVVFYKRIHRVFFVDSIPKSPSGKILRRQLR 540

Query: 541 ALLAASIPN 548
            LLAA IPN
Sbjct: 541 ELLAARIPN 545

BLAST of MS002251 vs. NCBI nr
Match: XP_038878634.1 (4-coumarate--CoA ligase 1-like [Benincasa hispida])

HSP 1 Score: 847.0 bits (2187), Expect = 8.8e-242
Identity = 435/554 (78.52%), Postives = 477/554 (86.10%), Query Frame = 0

Query: 2   AAADPPPEFIFRSKLPDITIPNHLPLHTYCFEKLPEFRDRPCLINAATGETHTYGEVHKT 61
           A  D  PEFIFRSKLP+I+IP HLPLHTY FEK+ EF +RPCLINA TGET TYGEVH+T
Sbjct: 3   ATEDSSPEFIFRSKLPEISIPTHLPLHTYLFEKVSEFTNRPCLINATTGETFTYGEVHET 62

Query: 62  ARRVAAGLHRMGIRKGDVIMLLLQNSPQFVLAFLGASFLGAVATMANPFFTATEVAKQAG 121
           +RRVAAGLH++GIRKGDVIMLLLQN+PQFV AFLGAS+LGA AT ANPFFT  E+ KQA 
Sbjct: 63  SRRVAAGLHKLGIRKGDVIMLLLQNTPQFVFAFLGASYLGAAATTANPFFTPAEITKQAA 122

Query: 122 SAGAKLIITQAAFAAKVKNLTCHENDVIIKVIYIDQPPPDD---DGGRHFSEL----MRA 181
           S+  KLIITQAAFA KVK L+  EN  IIKVI+ID    DD   DGG HFS L     + 
Sbjct: 123 SSSIKLIITQAAFAEKVKKLS-QENKAIIKVIFIDDDDDDDPPPDGGYHFSSLTDDVAKE 182

Query: 182 AEEEIPEVKISPDDVVALPYSSGTTGLPKGVMLTHRALVTSVAQQVDGENPHLNVRRDDV 241
            E E+ + K+SPDDVVALPYSSGTTGLPKGVMLTH+ LV  VAQQVDGENPH N++ DDV
Sbjct: 183 EEMEMGDAKVSPDDVVALPYSSGTTGLPKGVMLTHKGLVAIVAQQVDGENPHFNIQSDDV 242

Query: 242 VLCVLPLFHIYSLNCIMMCSLRVGAAILIVHKFDINSLVKFVPKYKVTVAPLVPPIVLAI 301
           VLCVLPLFHIYSLN IMMCSLRVGAAILIV KFD+N L+  +PKYKVT+AP+VPPIVLAI
Sbjct: 243 VLCVLPLFHIYSLNSIMMCSLRVGAAILIVQKFDVNLLLGLIPKYKVTIAPIVPPIVLAI 302

Query: 302 AESPAVESSDMSSIRMVLSGAAPLGKELEDAVKAKLPQAILGQGYRMTE-GLVFTMSLAF 361
            +SPAV+ SDMSS+R+V+SGAAPLGK LEDA + KLP  ILGQGY MTE G   TMSLAF
Sbjct: 303 VKSPAVDHSDMSSLRIVMSGAAPLGKNLEDAFRTKLPHVILGQGYGMTESGSAMTMSLAF 362

Query: 362 AKERFEVKSGACGTLMRNSEMKIINPQTGVSLPRNQPGEICIRSTQFMKGYLHDEEATKG 421
           AKE FE+KSG CGT+MRN+EMKIINPQTG SLPRNQ GEICI+S Q MKGYL+DEEATKG
Sbjct: 363 AKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQIMKGYLNDEEATKG 422

Query: 422 IIDKNGWLHSGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVV 481
           IIDK+GWLH+GD+GFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAV+
Sbjct: 423 IIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVI 482

Query: 482 PMKDEVAGEVPAAFIVRSDSSNIAEDEIKQYISKQVVFYKRINRVFFVDSIPKSSSGKIL 541
           PMKDEVAGEVP AFI RSD +NI EDEIKQYISKQVVFYKRINRVFFVDSIPKS SGKIL
Sbjct: 483 PMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKIL 542

Query: 542 RRQLRALLAASIPN 548
           RRQLRALLAASIPN
Sbjct: 543 RRQLRALLAASIPN 555

BLAST of MS002251 vs. NCBI nr
Match: XP_022987367.1 (4-coumarate--CoA ligase 1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 844.0 bits (2179), Expect = 7.5e-241
Identity = 436/549 (79.42%), Postives = 479/549 (87.25%), Query Frame = 0

Query: 1   MAAADPPPEFIFRSKLPDITIPNHLPLHTYCFEKLPEFRDRPCLINAATGETHTYGEVHK 60
           MAAADP PEFIFRSKLPD+TIPNHLPLHTYCFE++ EF+  PCLINAATG+ +TYGEVH 
Sbjct: 1   MAAADPSPEFIFRSKLPDLTIPNHLPLHTYCFERISEFKHNPCLINAATGDIYTYGEVHV 60

Query: 61  TARRVAAGLHRMGIRKGDVIMLLLQNSPQFVLAFLGASFLGAVATMANPFFTATEVAKQA 120
           TARRVAAGLH++GI KGDVIMLLLQN+PQF  AFLGASF  A+ATMANPFFT  E+AKQA
Sbjct: 61  TARRVAAGLHKLGIGKGDVIMLLLQNTPQFAFAFLGASFACAIATMANPFFTPAEIAKQA 120

Query: 121 GSAGAKLIITQAAFAAKVKNLTCHENDVIIKVIYIDQPPPDDDGGRHFSELM-RAAEEEI 180
            S+GAKLIITQAAFA KVKNL   EN  I+KVI+ID PP   +GG HFS L   A EEE+
Sbjct: 121 ASSGAKLIITQAAFAEKVKNLP-QENQGILKVIFIDSPP---EGGCHFSSLTDEAKEEEL 180

Query: 181 PEVKISPDDVVALPYSSGTTGLPKGVMLTHRALVTSVAQQVDGENPHLNVRRDDVVLCVL 240
            +VK SPDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENPH ++R +DVVLCVL
Sbjct: 181 TDVKFSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVL 240

Query: 241 PLFHIYSLNCIMMCSLRVGAAILIVHKFDINSLVKFVPKYKVTVAPLVPPIVLAIAESPA 300
           PLFHIYSL+ I+M SLRVGAAIL+V KFDINSLV  VPKY+VT+A +VPPIVLA+ +SP 
Sbjct: 241 PLFHIYSLSSIVMSSLRVGAAILLVQKFDINSLVALVPKYRVTIATVVPPIVLAMVKSPV 300

Query: 301 VESSDMSSIRMVLSGAAPLGKELEDAVKAKLPQAILGQGYRMTE-GLVFTMSLAFAKERF 360
           V+ SDMSSIR+VLSGAAPLGK LEDA +AK P AILGQGY MTE G V TMSLA AKERF
Sbjct: 301 VDCSDMSSIRIVLSGAAPLGKNLEDAFRAKFPHAILGQGYGMTESGSVLTMSLALAKERF 360

Query: 361 EVKSGACGTLMRNSEMKIINPQTGVSLPRNQPGEICIRSTQFMKGYLHDEEATKGIIDKN 420
            +KSGACGTLMRN+EMKI+NP+TGVSL RNQPGEI +RS Q MKGYL+DEEATKGIID +
Sbjct: 361 AIKSGACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDGD 420

Query: 421 GWLHSGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVVPMKDE 480
           GWLH+GDIG VDDDDEVFIVDRLKELIKYKGFQVAPAELE LLIS+ HI D AV+PMKDE
Sbjct: 421 GWLHTGDIGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDE 480

Query: 481 VAGEVPAAFIVRSDSSNIAEDEIKQYISKQVVFYKRINRVFFVDSIPKSSSGKILRRQLR 540
           VAGE P AFIVRSD SNI EDEIK+YISKQVVFYKRINRVFFVDSIPKS SGKILRRQLR
Sbjct: 481 VAGEAPVAFIVRSDGSNITEDEIKEYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLR 540

Query: 541 ALLAASIPN 548
           ALL ASIPN
Sbjct: 541 ALLPASIPN 545

BLAST of MS002251 vs. NCBI nr
Match: XP_022933955.1 (4-coumarate--CoA ligase 2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 843.6 bits (2178), Expect = 9.7e-241
Identity = 442/549 (80.51%), Postives = 481/549 (87.61%), Query Frame = 0

Query: 1   MAAADPPPEFIFRSKLPDITIPNHLPLHTYCFEKLPEFRDRPCLINAATGETHTYGEVHK 60
           MAAADP PEFIFRSKLPDITIP+HLPLHTYCFE L +F+ RPCLIN ATG+T+TY EVH 
Sbjct: 1   MAAADPSPEFIFRSKLPDITIPDHLPLHTYCFENLSQFKHRPCLINGATGDTYTYEEVHV 60

Query: 61  TARRVAAGLHRMGIRKGDVIMLLLQNSPQFVLAFLGASFLGAVATMANPFFTATEVAKQA 120
           TARRVAAGLH++GI KGDVIMLLLQNSPQFVLAFLGAS++GAVATMANPFFT  E+AK  
Sbjct: 61  TARRVAAGLHKLGIGKGDVIMLLLQNSPQFVLAFLGASYIGAVATMANPFFTPPEIAKHV 120

Query: 121 GSAGAKLIITQAAFAAKVKNLTCHENDVIIKVIYIDQPPPDDDGGRHFSELM-RAAEEEI 180
            S+G KLIITQAA A KVK+L   +N   IK+I+ID PP   DG  HFS L     EEE+
Sbjct: 121 ASSGPKLIITQAAIAEKVKSLV-DKNGASIKLIFIDPPP---DGEVHFSVLTDYVKEEEM 180

Query: 181 PEVKISPDDVVALPYSSGTTGLPKGVMLTHRALVTSVAQQVDGENPHLNVRRDDVVLCVL 240
           P+VKISP+DVVALPYSSGTTGLPKGVMLTHR LVTSVAQQVDGENPHL +R DDVVLCVL
Sbjct: 181 PDVKISPNDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPHLYIRSDDVVLCVL 240

Query: 241 PLFHIYSLNCIMMCSLRVGAAILIVHKFDINSLVKFVPKYKVTVAPLVPPIVLAIAESPA 300
           PLFHIYSLN IMMCSLRVGAAILIV KFDIN +V+ V KYKVT+APLVPPIVLAIA+SPA
Sbjct: 241 PLFHIYSLNSIMMCSLRVGAAILIVQKFDINYVVELVGKYKVTIAPLVPPIVLAIAKSPA 300

Query: 301 VESSDMSSIRMVLSGAAPLGKELEDAVKAKLPQAILGQGYRMTE-GLVFTMSLAFAKERF 360
           V   +MSSIR+VLSGAAPLGKELEDA +AKLP AILGQGY MTE G   T+SLAFAKERF
Sbjct: 301 VGGWEMSSIRLVLSGAAPLGKELEDAFRAKLPDAILGQGYGMTEAGSALTLSLAFAKERF 360

Query: 361 EVKSGACGTLMRNSEMKIINPQTGVSLPRNQPGEICIRSTQFMKGYLHDEEATKGIIDKN 420
            +KSGACGTLMRNS+MKI+NPQTG SLPRNQ GEI IRS   MKGYL++EEATK IID++
Sbjct: 361 PIKSGACGTLMRNSDMKIVNPQTGASLPRNQAGEIWIRSPHVMKGYLNNEEATKAIIDEH 420

Query: 421 GWLHSGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVVPMKDE 480
           GWLH+GDIGFVD+DDEVFIVDRLKELIKYKGFQVAPAELEALLISH HIADAAV+PMK+E
Sbjct: 421 GWLHTGDIGFVDEDDEVFIVDRLKELIKYKGFQVAPAELEALLISHTHIADAAVIPMKNE 480

Query: 481 VAGEVPAAFIVRSDSSNIAEDEIKQYISKQVVFYKRINRVFFVDSIPKSSSGKILRRQLR 540
           VAGEVP AFIVRS  SNI EDEIKQYISKQVVFYKRI+RVFFVDSIPKS SGKILRRQLR
Sbjct: 481 VAGEVPVAFIVRSGGSNITEDEIKQYISKQVVFYKRIHRVFFVDSIPKSPSGKILRRQLR 540

Query: 541 ALLAASIPN 548
            LLAA IPN
Sbjct: 541 ELLAARIPN 545

BLAST of MS002251 vs. ExPASy Swiss-Prot
Match: M4ISH0 (4-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1)

HSP 1 Score: 733.0 bits (1891), Expect = 2.4e-210
Identity = 370/538 (68.77%), Postives = 445/538 (82.71%), Query Frame = 0

Query: 5   DPPPEFIFRSKLPDITIPNHLPLHTYCFEKLPEFRDRPCLINAATGETHTYGEVHKTARR 64
           D   EFIFRSKLPDI IPNHLPLH+YCFE + +F+DRPCLIN ATGE  TY +V  T+R+
Sbjct: 8   DHQEEFIFRSKLPDIYIPNHLPLHSYCFENISQFKDRPCLINGATGEIITYADVDLTSRK 67

Query: 65  VAAGLHRMGIRKGDVIMLLLQNSPQFVLAFLGASFLGAVATMANPFFTATEVAKQAGSAG 124
           VAAGL ++GI++GDVIMLLLQNSP+FV AFL AS++GA+ T ANPF+T  EVAKQA ++ 
Sbjct: 68  VAAGLDKLGIKQGDVIMLLLQNSPEFVYAFLAASYIGAIITTANPFYTPAEVAKQAAASK 127

Query: 125 AKLIITQAAFAAKVKNLTCHENDVIIKVIYIDQPPPDDDGGRHFSELMRAAEEEIPEVKI 184
            KL+IT A +  KVK  T  E+ V  KV+ +D PPP+ +   HFSEL +A E EIP VKI
Sbjct: 128 TKLVITLAGYIDKVKEFTGGESGV--KVMCVDAPPPESE-CLHFSELTQADETEIPAVKI 187

Query: 185 SPDDVVALPYSSGTTGLPKGVMLTHRALVTSVAQQVDGENPHLNVRRDDVVLCVLPLFHI 244
            PDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDG+NP+L   ++DV+LCVLPLFHI
Sbjct: 188 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFHQNDVILCVLPLFHI 247

Query: 245 YSLNCIMMCSLRVGAAILIVHKFDINSLVKFVPKYKVTVAPLVPPIVLAIAESPAVESSD 304
           YSLN I++C LRVGAAILI+ KF+I+ L++ + K+KVT+AP VPPIVL++A+ P +   D
Sbjct: 248 YSLNSILLCGLRVGAAILIMQKFEISKLLELIEKFKVTIAPFVPPIVLSVAKCPDLHRYD 307

Query: 305 MSSIRMVLSGAAPLGKELEDAVKAKLPQAILGQGYRMTE-GLVFTMSLAFAKERFEVKSG 364
           +SSIR V+SG AP+GKELEDAVK KLP A LGQGY MTE G V +M LAFAKE F +KSG
Sbjct: 308 LSSIRTVMSGGAPMGKELEDAVKEKLPHAKLGQGYGMTEAGPVLSMCLAFAKEPFPIKSG 367

Query: 365 ACGTLMRNSEMKIINPQTGVSLPRNQPGEICIRSTQFMKGYLHDEEATKGIIDKNGWLHS 424
           ACGT++RN+EMKI++P TG SLPRNQ GEICIR  Q MKGY++D EATKG ID+ GWLH+
Sbjct: 368 ACGTVVRNAEMKIVDPDTGASLPRNQSGEICIRGKQIMKGYINDAEATKGTIDEGGWLHT 427

Query: 425 GDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVVPMKDEVAGEV 484
           GDIGF+D+DDE+FIVDRLKELIKYKGFQVAPAELE++LISH +I DAAVVPMKDE AGEV
Sbjct: 428 GDIGFIDNDDELFIVDRLKELIKYKGFQVAPAELESMLISHPNITDAAVVPMKDEAAGEV 487

Query: 485 PAAFIVRSDSSNIAEDEIKQYISKQVVFYKRINRVFFVDSIPKSSSGKILRRQLRALL 542
           P AF+VRS+ S I E++IKQYISKQVVFYKRIN+ FF++ IPK+ SGKILR+ LRA L
Sbjct: 488 PVAFVVRSNGSKITEEDIKQYISKQVVFYKRINKAFFIEEIPKNPSGKILRKILRAKL 542

BLAST of MS002251 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 731.9 bits (1888), Expect = 5.4e-210
Identity = 369/540 (68.33%), Postives = 442/540 (81.85%), Query Frame = 0

Query: 9   EFIFRSKLPDITIPNHLPLHTYCFEKLPEFRDRPCLINAATGETHTYGEVHKTARRVAAG 68
           + IFRSKLPDI IP HLPLH+YCFE + EF  RPCLIN A  + +TY EV  T R+VA G
Sbjct: 14  DLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTCRKVAVG 73

Query: 69  LHRMGIRKGDVIMLLLQNSPQFVLAFLGASFLGAVATMANPFFTATEVAKQAGSAGAKLI 128
           L+++GI++ D IM+LL NSP+FV AF+GAS+LGA++TMANP FT  EV KQA ++ AK+I
Sbjct: 74  LNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASSAKII 133

Query: 129 ITQAAFAAKVKNLTCHENDVIIKVIYIDQPPPDDDGGRHFSELMRAAEEEIPEVKISPDD 188
           ITQ+ F  KVK+    ENDV  KVI ID  P   +G  HFSEL ++ E EIPEVKI PDD
Sbjct: 134 ITQSCFVGKVKDY-ASENDV--KVICIDSAP---EGCLHFSELTQSDEHEIPEVKIQPDD 193

Query: 189 VVALPYSSGTTGLPKGVMLTHRALVTSVAQQVDGENPHLNVRRDDVVLCVLPLFHIYSLN 248
           VVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGEN +L +  +DV++CVLPLFHIYSLN
Sbjct: 194 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLN 253

Query: 249 CIMMCSLRVGAAILIVHKFDINSLVKFVPKYKVTVAPLVPPIVLAIAESPAVESSDMSSI 308
            I++C LRVGAAILI+ KFDI   ++ + KYKV++ P VPPIVLAIA+SP V+S D+SS+
Sbjct: 254 SILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSV 313

Query: 309 RMVLSGAAPLGKELEDAVKAKLPQAILGQGYRMTE-GLVFTMSLAFAKERFEVKSGACGT 368
           R V+SGAAPLGKELEDAV+ K P A LGQGY MTE G V  M LAFAKE F++KSGACGT
Sbjct: 314 RTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 373

Query: 369 LMRNSEMKIINPQTGVSLPRNQPGEICIRSTQFMKGYLHDEEATKGIIDKNGWLHSGDIG 428
           ++RN+EMKI++P TG SLPRNQPGEICIR  Q MKGYL+D EAT   IDK GWLH+GDIG
Sbjct: 374 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIG 433

Query: 429 FVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVVPMKDEVAGEVPAAF 488
           F+D+DDE+FIVDRLKELIKYKGFQVAPAE+EALL++H +I+DAAVVPMKDE AGEVP AF
Sbjct: 434 FIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAF 493

Query: 489 IVRSDSSNIAEDEIKQYISKQVVFYKRINRVFFVDSIPKSSSGKILRRQLRALLAASIPN 548
           +VRS+ S I EDE+K +ISKQV+FYKR+ RVFFV+++PKS SGKILR+ LRA LAA +PN
Sbjct: 494 VVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGVPN 547

BLAST of MS002251 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 1.6e-209
Identity = 370/540 (68.52%), Postives = 441/540 (81.67%), Query Frame = 0

Query: 9   EFIFRSKLPDITIPNHLPLHTYCFEKLPEFRDRPCLINAATGETHTYGEVHKTARRVAAG 68
           + IFRSKLPDI IPNHLPLH+YCFE + EF  RPCLIN A  + +TY +V   +R+VAAG
Sbjct: 9   DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRKVAAG 68

Query: 69  LHRMGIRKGDVIMLLLQNSPQFVLAFLGASFLGAVATMANPFFTATEVAKQAGSAGAKLI 128
           LH+ GI+  D IM+LL NSP+FV AF+GAS+LGA++TMANP FT  EV KQA ++ AK+I
Sbjct: 69  LHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKII 128

Query: 129 ITQAAFAAKVKNLTCHENDVIIKVIYIDQPPPDDDGGRHFSELMRAAEEEIPEVKISPDD 188
           +TQA    KVK+    ENDV  K+I ID  P   +G  HFS L +A E +IPEV+I PDD
Sbjct: 129 VTQACHVNKVKDY-AFENDV--KIICIDSAP---EGCLHFSVLTQANEHDIPEVEIQPDD 188

Query: 189 VVALPYSSGTTGLPKGVMLTHRALVTSVAQQVDGENPHLNVRRDDVVLCVLPLFHIYSLN 248
           VVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENP+L +  +DV+LCVLPLFHIYSLN
Sbjct: 189 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLN 248

Query: 249 CIMMCSLRVGAAILIVHKFDINSLVKFVPKYKVTVAPLVPPIVLAIAESPAVESSDMSSI 308
            +++C LRVGAAILI+ KFDI S ++ + +YKVT+ P VPPIVLAIA+SP V+  D+SS+
Sbjct: 249 SVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSSV 308

Query: 309 RMVLSGAAPLGKELEDAVKAKLPQAILGQGYRMTE-GLVFTMSLAFAKERFEVKSGACGT 368
           R V+SGAAPLGKELED V+AK P A LGQGY MTE G V  M LAFAKE FE+KSGACGT
Sbjct: 309 RTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGT 368

Query: 369 LMRNSEMKIINPQTGVSLPRNQPGEICIRSTQFMKGYLHDEEATKGIIDKNGWLHSGDIG 428
           ++RN+EMKI++P+TG SLPRNQ GEICIR  Q MKGYL+D EAT   IDK GWL++GDIG
Sbjct: 369 VVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIG 428

Query: 429 FVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVVPMKDEVAGEVPAAF 488
           ++DDDDE+FIVDRLKELIKYKGFQVAPAELEALL++H +I+DAAVVPMKDE AGEVP AF
Sbjct: 429 YIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAF 488

Query: 489 IVRSDSSNIAEDEIKQYISKQVVFYKRINRVFFVDSIPKSSSGKILRRQLRALLAASIPN 548
           +VRS+ S I EDE+K +ISKQV+FYKRI RVFFVD+IPKS SGKILR+ LRA LAA +PN
Sbjct: 489 VVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLPN 542

BLAST of MS002251 vs. ExPASy Swiss-Prot
Match: P31684 (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 2.1e-209
Identity = 369/540 (68.33%), Postives = 443/540 (82.04%), Query Frame = 0

Query: 9   EFIFRSKLPDITIPNHLPLHTYCFEKLPEFRDRPCLINAATGETHTYGEVHKTARRVAAG 68
           + IFRSKLPDI IP HLPLH+YCFE L EF  RPCLI+ A    +TY EV  T+R+VA G
Sbjct: 12  DLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVAVG 71

Query: 69  LHRMGIRKGDVIMLLLQNSPQFVLAFLGASFLGAVATMANPFFTATEVAKQAGSAGAKLI 128
           L+++GI++ D IM+LL N P+FV AF+GAS+LGA++TMANP FT  EV KQA ++ AK++
Sbjct: 72  LNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKIV 131

Query: 129 ITQAAFAAKVKNLTCHENDVIIKVIYIDQPPPDDDGGRHFSELMRAAEEEIPEVKISPDD 188
           ITQA FA KVK+    END  +KVI +D  P   +G  HFSEL+++ E EIP+VKI PDD
Sbjct: 132 ITQACFAGKVKDYAI-END--LKVICVDSVP---EGCVHFSELIQSDEHEIPDVKIQPDD 191

Query: 189 VVALPYSSGTTGLPKGVMLTHRALVTSVAQQVDGENPHLNVRRDDVVLCVLPLFHIYSLN 248
           VVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGEN +L +  DDV++CVLPLFHIYSLN
Sbjct: 192 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIYSLN 251

Query: 249 CIMMCSLRVGAAILIVHKFDINSLVKFVPKYKVTVAPLVPPIVLAIAESPAVESSDMSSI 308
            +++C+LRVGAAILI+ KFDI   ++ +PK+KVT+ P VPPIVLAIA+SP V++ D+SS+
Sbjct: 252 SVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDLSSV 311

Query: 309 RMVLSGAAPLGKELEDAVKAKLPQAILGQGYRMTE-GLVFTMSLAFAKERFEVKSGACGT 368
           R V+SGAAPLGKELEDAV+AK P A LGQGY MTE G V  M LAFAKE F++KSGACGT
Sbjct: 312 RTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 371

Query: 369 LMRNSEMKIINPQTGVSLPRNQPGEICIRSTQFMKGYLHDEEATKGIIDKNGWLHSGDIG 428
           ++RN+EMKI++P TG SLPRNQPGEICIR  Q MKGYL+D EAT   I+K GWLH+GDIG
Sbjct: 372 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTGDIG 431

Query: 429 FVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVVPMKDEVAGEVPAAF 488
           F+DDDDE+FIVDRLKELIKYKGFQVAPAELEALLI+H  I+DAAVVPM DE AGEVP AF
Sbjct: 432 FIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVPVAF 491

Query: 489 IVRSDSSNIAEDEIKQYISKQVVFYKRINRVFFVDSIPKSSSGKILRRQLRALLAASIPN 548
           +VRS+ S I EDE+K +ISKQV+FYKRI RVFFV+++PKS SGKILR+ LRA LAA I N
Sbjct: 492 VVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAGISN 545

BLAST of MS002251 vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 2.7e-209
Identity = 369/540 (68.33%), Postives = 442/540 (81.85%), Query Frame = 0

Query: 9   EFIFRSKLPDITIPNHLPLHTYCFEKLPEFRDRPCLINAATGETHTYGEVHKTARRVAAG 68
           + IFRSKLPDI IP HLPLH+YCFE L EF  RPCLI+ A    +TY EV  T+R+VA G
Sbjct: 12  DLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVAVG 71

Query: 69  LHRMGIRKGDVIMLLLQNSPQFVLAFLGASFLGAVATMANPFFTATEVAKQAGSAGAKLI 128
           L+++GI++ D IM+LL N P+FV AF+GAS+LGA++TMANP FT  EV KQA ++ AK++
Sbjct: 72  LNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKIV 131

Query: 129 ITQAAFAAKVKNLTCHENDVIIKVIYIDQPPPDDDGGRHFSELMRAAEEEIPEVKISPDD 188
           ITQA FA KVK+    END  +KVI +D  P   +G  HFSEL+++ E EIP+VKI PDD
Sbjct: 132 ITQACFAGKVKDYAI-END--LKVICVDSAP---EGCVHFSELIQSDEHEIPDVKIQPDD 191

Query: 189 VVALPYSSGTTGLPKGVMLTHRALVTSVAQQVDGENPHLNVRRDDVVLCVLPLFHIYSLN 248
           VVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGEN +L +  DDV++CVLPLFHIYSLN
Sbjct: 192 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIYSLN 251

Query: 249 CIMMCSLRVGAAILIVHKFDINSLVKFVPKYKVTVAPLVPPIVLAIAESPAVESSDMSSI 308
            +++C+LRVGAAILI+ KFDI   ++ +PK+KVT+ P VPPIVLAIA+SP V + D+SS+
Sbjct: 252 SVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDLSSV 311

Query: 309 RMVLSGAAPLGKELEDAVKAKLPQAILGQGYRMTE-GLVFTMSLAFAKERFEVKSGACGT 368
           R V+SGAAPLGKELEDAV+AK P A LGQGY MTE G V  M LAFAKE F++KSGACGT
Sbjct: 312 RTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 371

Query: 369 LMRNSEMKIINPQTGVSLPRNQPGEICIRSTQFMKGYLHDEEATKGIIDKNGWLHSGDIG 428
           ++RN+EMKI++P TG SLPRNQPGEICIR  Q MKGYL+D EAT   I+K GWLH+GDIG
Sbjct: 372 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTGDIG 431

Query: 429 FVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVVPMKDEVAGEVPAAF 488
           F+DDDDE+FIVDRLKELIKYKGFQVAPAELEALLI+H  I+DAAVVPM DE AGEVP AF
Sbjct: 432 FIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVPVAF 491

Query: 489 IVRSDSSNIAEDEIKQYISKQVVFYKRINRVFFVDSIPKSSSGKILRRQLRALLAASIPN 548
           +VRS+ S I EDE+K +ISKQV+FYKRI RVFFV+++PKS SGKILR+ LRA LAA I N
Sbjct: 492 VVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAGISN 545

BLAST of MS002251 vs. ExPASy TrEMBL
Match: A0A6J1JA64 (4-coumarate--CoA ligase 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484944 PE=4 SV=1)

HSP 1 Score: 844.0 bits (2179), Expect = 3.6e-241
Identity = 436/549 (79.42%), Postives = 479/549 (87.25%), Query Frame = 0

Query: 1   MAAADPPPEFIFRSKLPDITIPNHLPLHTYCFEKLPEFRDRPCLINAATGETHTYGEVHK 60
           MAAADP PEFIFRSKLPD+TIPNHLPLHTYCFE++ EF+  PCLINAATG+ +TYGEVH 
Sbjct: 1   MAAADPSPEFIFRSKLPDLTIPNHLPLHTYCFERISEFKHNPCLINAATGDIYTYGEVHV 60

Query: 61  TARRVAAGLHRMGIRKGDVIMLLLQNSPQFVLAFLGASFLGAVATMANPFFTATEVAKQA 120
           TARRVAAGLH++GI KGDVIMLLLQN+PQF  AFLGASF  A+ATMANPFFT  E+AKQA
Sbjct: 61  TARRVAAGLHKLGIGKGDVIMLLLQNTPQFAFAFLGASFACAIATMANPFFTPAEIAKQA 120

Query: 121 GSAGAKLIITQAAFAAKVKNLTCHENDVIIKVIYIDQPPPDDDGGRHFSELM-RAAEEEI 180
            S+GAKLIITQAAFA KVKNL   EN  I+KVI+ID PP   +GG HFS L   A EEE+
Sbjct: 121 ASSGAKLIITQAAFAEKVKNLP-QENQGILKVIFIDSPP---EGGCHFSSLTDEAKEEEL 180

Query: 181 PEVKISPDDVVALPYSSGTTGLPKGVMLTHRALVTSVAQQVDGENPHLNVRRDDVVLCVL 240
            +VK SPDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENPH ++R +DVVLCVL
Sbjct: 181 TDVKFSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVL 240

Query: 241 PLFHIYSLNCIMMCSLRVGAAILIVHKFDINSLVKFVPKYKVTVAPLVPPIVLAIAESPA 300
           PLFHIYSL+ I+M SLRVGAAIL+V KFDINSLV  VPKY+VT+A +VPPIVLA+ +SP 
Sbjct: 241 PLFHIYSLSSIVMSSLRVGAAILLVQKFDINSLVALVPKYRVTIATVVPPIVLAMVKSPV 300

Query: 301 VESSDMSSIRMVLSGAAPLGKELEDAVKAKLPQAILGQGYRMTE-GLVFTMSLAFAKERF 360
           V+ SDMSSIR+VLSGAAPLGK LEDA +AK P AILGQGY MTE G V TMSLA AKERF
Sbjct: 301 VDCSDMSSIRIVLSGAAPLGKNLEDAFRAKFPHAILGQGYGMTESGSVLTMSLALAKERF 360

Query: 361 EVKSGACGTLMRNSEMKIINPQTGVSLPRNQPGEICIRSTQFMKGYLHDEEATKGIIDKN 420
            +KSGACGTLMRN+EMKI+NP+TGVSL RNQPGEI +RS Q MKGYL+DEEATKGIID +
Sbjct: 361 AIKSGACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDGD 420

Query: 421 GWLHSGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVVPMKDE 480
           GWLH+GDIG VDDDDEVFIVDRLKELIKYKGFQVAPAELE LLIS+ HI D AV+PMKDE
Sbjct: 421 GWLHTGDIGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDE 480

Query: 481 VAGEVPAAFIVRSDSSNIAEDEIKQYISKQVVFYKRINRVFFVDSIPKSSSGKILRRQLR 540
           VAGE P AFIVRSD SNI EDEIK+YISKQVVFYKRINRVFFVDSIPKS SGKILRRQLR
Sbjct: 481 VAGEAPVAFIVRSDGSNITEDEIKEYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLR 540

Query: 541 ALLAASIPN 548
           ALL ASIPN
Sbjct: 541 ALLPASIPN 545

BLAST of MS002251 vs. ExPASy TrEMBL
Match: A0A6J1KTJ2 (4-coumarate--CoA ligase 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497098 PE=4 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 4.7e-241
Identity = 440/549 (80.15%), Postives = 483/549 (87.98%), Query Frame = 0

Query: 1   MAAADPPPEFIFRSKLPDITIPNHLPLHTYCFEKLPEFRDRPCLINAATGETHTYGEVHK 60
           MAAAD  PEFIFRSKLPDITIP+HLPLHTYCFE + +F+ RPCLIN ATG+T+TY EVH 
Sbjct: 1   MAAADLSPEFIFRSKLPDITIPDHLPLHTYCFENISQFKHRPCLINGATGDTYTYDEVHV 60

Query: 61  TARRVAAGLHRMGIRKGDVIMLLLQNSPQFVLAFLGASFLGAVATMANPFFTATEVAKQA 120
           T+RRVAAGLH++GI KGDVIMLLLQNSP FVLAFLGAS++GAV TMANPFFT  E+AK  
Sbjct: 61  TSRRVAAGLHKLGIGKGDVIMLLLQNSPHFVLAFLGASYIGAVVTMANPFFTLPEIAKHV 120

Query: 121 GSAGAKLIITQAAFAAKVKNLTCHENDVIIKVIYIDQPPPDDDGGRHFSELM-RAAEEEI 180
            S+GAKLIITQAAFA KVK+L   +N V IK+I+ID PP   DG  HFS L     EE++
Sbjct: 121 ASSGAKLIITQAAFAEKVKSLV-DKNGVSIKLIFIDPPP---DGEAHFSVLTDDVKEEDM 180

Query: 181 PEVKISPDDVVALPYSSGTTGLPKGVMLTHRALVTSVAQQVDGENPHLNVRRDDVVLCVL 240
           P+VKISP+DVVALPYSSGTTGLPKGVMLTHR LVTSVAQQVDGENPHL +R DDVVLCVL
Sbjct: 181 PDVKISPNDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPHLYIRSDDVVLCVL 240

Query: 241 PLFHIYSLNCIMMCSLRVGAAILIVHKFDINSLVKFVPKYKVTVAPLVPPIVLAIAESPA 300
           PLFHIYSLN IMMCSLRVGAAILIV KFDIN +V+ V KYKVT+APLVPPIVLAIA+SPA
Sbjct: 241 PLFHIYSLNSIMMCSLRVGAAILIVQKFDINYVVELVGKYKVTIAPLVPPIVLAIAKSPA 300

Query: 301 VESSDMSSIRMVLSGAAPLGKELEDAVKAKLPQAILGQGYRMTE-GLVFTMSLAFAKERF 360
           V   +MSSIRMVLSGAAPLGKELEDA +AKLP AILGQGY MTE G   T+SLAFAKERF
Sbjct: 301 VGGWEMSSIRMVLSGAAPLGKELEDAFRAKLPDAILGQGYGMTEAGSALTLSLAFAKERF 360

Query: 361 EVKSGACGTLMRNSEMKIINPQTGVSLPRNQPGEICIRSTQFMKGYLHDEEATKGIIDKN 420
           ++KSGACGTLMRNSEMKI++PQTG SLPRNQPGEI IRS   MKGYL++EEATK IID++
Sbjct: 361 QIKSGACGTLMRNSEMKIVSPQTGASLPRNQPGEIWIRSPHVMKGYLNNEEATKAIIDEH 420

Query: 421 GWLHSGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVVPMKDE 480
           GWLH+GDIG+VD+DDEVFIVDRLKELIKYKGFQVAPAELEALLISH HIADAAV+PMK+E
Sbjct: 421 GWLHTGDIGYVDEDDEVFIVDRLKELIKYKGFQVAPAELEALLISHTHIADAAVIPMKNE 480

Query: 481 VAGEVPAAFIVRSDSSNIAEDEIKQYISKQVVFYKRINRVFFVDSIPKSSSGKILRRQLR 540
           VAGEVP AFIVRS  SNI EDEIKQYISKQVVFYKRI+RVFFVDSIPKS SGKILRRQLR
Sbjct: 481 VAGEVPVAFIVRSGGSNITEDEIKQYISKQVVFYKRIHRVFFVDSIPKSPSGKILRRQLR 540

Query: 541 ALLAASIPN 548
            LLAA IPN
Sbjct: 541 ELLAARIPN 545

BLAST of MS002251 vs. ExPASy TrEMBL
Match: A0A6J1F6A3 (4-coumarate--CoA ligase 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441212 PE=4 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 4.7e-241
Identity = 442/549 (80.51%), Postives = 481/549 (87.61%), Query Frame = 0

Query: 1   MAAADPPPEFIFRSKLPDITIPNHLPLHTYCFEKLPEFRDRPCLINAATGETHTYGEVHK 60
           MAAADP PEFIFRSKLPDITIP+HLPLHTYCFE L +F+ RPCLIN ATG+T+TY EVH 
Sbjct: 1   MAAADPSPEFIFRSKLPDITIPDHLPLHTYCFENLSQFKHRPCLINGATGDTYTYEEVHV 60

Query: 61  TARRVAAGLHRMGIRKGDVIMLLLQNSPQFVLAFLGASFLGAVATMANPFFTATEVAKQA 120
           TARRVAAGLH++GI KGDVIMLLLQNSPQFVLAFLGAS++GAVATMANPFFT  E+AK  
Sbjct: 61  TARRVAAGLHKLGIGKGDVIMLLLQNSPQFVLAFLGASYIGAVATMANPFFTPPEIAKHV 120

Query: 121 GSAGAKLIITQAAFAAKVKNLTCHENDVIIKVIYIDQPPPDDDGGRHFSELM-RAAEEEI 180
            S+G KLIITQAA A KVK+L   +N   IK+I+ID PP   DG  HFS L     EEE+
Sbjct: 121 ASSGPKLIITQAAIAEKVKSLV-DKNGASIKLIFIDPPP---DGEVHFSVLTDYVKEEEM 180

Query: 181 PEVKISPDDVVALPYSSGTTGLPKGVMLTHRALVTSVAQQVDGENPHLNVRRDDVVLCVL 240
           P+VKISP+DVVALPYSSGTTGLPKGVMLTHR LVTSVAQQVDGENPHL +R DDVVLCVL
Sbjct: 181 PDVKISPNDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPHLYIRSDDVVLCVL 240

Query: 241 PLFHIYSLNCIMMCSLRVGAAILIVHKFDINSLVKFVPKYKVTVAPLVPPIVLAIAESPA 300
           PLFHIYSLN IMMCSLRVGAAILIV KFDIN +V+ V KYKVT+APLVPPIVLAIA+SPA
Sbjct: 241 PLFHIYSLNSIMMCSLRVGAAILIVQKFDINYVVELVGKYKVTIAPLVPPIVLAIAKSPA 300

Query: 301 VESSDMSSIRMVLSGAAPLGKELEDAVKAKLPQAILGQGYRMTE-GLVFTMSLAFAKERF 360
           V   +MSSIR+VLSGAAPLGKELEDA +AKLP AILGQGY MTE G   T+SLAFAKERF
Sbjct: 301 VGGWEMSSIRLVLSGAAPLGKELEDAFRAKLPDAILGQGYGMTEAGSALTLSLAFAKERF 360

Query: 361 EVKSGACGTLMRNSEMKIINPQTGVSLPRNQPGEICIRSTQFMKGYLHDEEATKGIIDKN 420
            +KSGACGTLMRNS+MKI+NPQTG SLPRNQ GEI IRS   MKGYL++EEATK IID++
Sbjct: 361 PIKSGACGTLMRNSDMKIVNPQTGASLPRNQAGEIWIRSPHVMKGYLNNEEATKAIIDEH 420

Query: 421 GWLHSGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVVPMKDE 480
           GWLH+GDIGFVD+DDEVFIVDRLKELIKYKGFQVAPAELEALLISH HIADAAV+PMK+E
Sbjct: 421 GWLHTGDIGFVDEDDEVFIVDRLKELIKYKGFQVAPAELEALLISHTHIADAAVIPMKNE 480

Query: 481 VAGEVPAAFIVRSDSSNIAEDEIKQYISKQVVFYKRINRVFFVDSIPKSSSGKILRRQLR 540
           VAGEVP AFIVRS  SNI EDEIKQYISKQVVFYKRI+RVFFVDSIPKS SGKILRRQLR
Sbjct: 481 VAGEVPVAFIVRSGGSNITEDEIKQYISKQVVFYKRIHRVFFVDSIPKSPSGKILRRQLR 540

Query: 541 ALLAASIPN 548
            LLAA IPN
Sbjct: 541 ELLAARIPN 545

BLAST of MS002251 vs. ExPASy TrEMBL
Match: A0A6J1E5C5 (4-coumarate--CoA ligase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429639 PE=4 SV=1)

HSP 1 Score: 831.6 bits (2147), Expect = 1.9e-237
Identity = 429/549 (78.14%), Postives = 478/549 (87.07%), Query Frame = 0

Query: 1   MAAADPPPEFIFRSKLPDITIPNHLPLHTYCFEKLPEFRDRPCLINAATGETHTYGEVHK 60
           MAAAD  PEFIFRSKLPD+TIPNHLPLHTYCFE++ +F+  PCLINAATG+ +TYGEVH 
Sbjct: 1   MAAADSSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVHV 60

Query: 61  TARRVAAGLHRMGIRKGDVIMLLLQNSPQFVLAFLGASFLGAVATMANPFFTATEVAKQA 120
           TARRVAAG H++GI KGDVIMLLLQN+PQFV AFLGASF  A+ATMANPFFT  E+AKQA
Sbjct: 61  TARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQA 120

Query: 121 GSAGAKLIITQAAFAAKVKNLTCHENDVIIKVIYIDQPPPDDDGGRHFSELM-RAAEEEI 180
            S+GAKLIITQAAFA KVK L   EN  I+KVI+ID PP   +GG HF+ L   A EEE+
Sbjct: 121 ASSGAKLIITQAAFAEKVKKLP-QENQGILKVIFIDSPP---EGGCHFTSLTDEAKEEEM 180

Query: 181 PEVKISPDDVVALPYSSGTTGLPKGVMLTHRALVTSVAQQVDGENPHLNVRRDDVVLCVL 240
            +VK SPDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENPH ++R +DVVLCVL
Sbjct: 181 ADVKFSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVL 240

Query: 241 PLFHIYSLNCIMMCSLRVGAAILIVHKFDINSLVKFVPKYKVTVAPLVPPIVLAIAESPA 300
           PLFHIYSL+ I+M SLRVGAAILIV KFDINSLV  VPKY+VT+A +VPPIVLA+ +SP 
Sbjct: 241 PLFHIYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAMVKSPV 300

Query: 301 VESSDMSSIRMVLSGAAPLGKELEDAVKAKLPQAILGQGYRMTE-GLVFTMSLAFAKERF 360
           V+ SDMSSIR+VLSGAAPLGK LEDA +AKLP AIL QGY MTE G V TMSLA A+E+F
Sbjct: 301 VDHSDMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILAQGYGMTESGSVLTMSLALAREKF 360

Query: 361 EVKSGACGTLMRNSEMKIINPQTGVSLPRNQPGEICIRSTQFMKGYLHDEEATKGIIDKN 420
            +KSGACGTLMRN+EMKI+NP+ GVSL RNQPGEI +RS Q MKGYL+DEEATKG+ID++
Sbjct: 361 AIKSGACGTLMRNAEMKIVNPENGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGMIDES 420

Query: 421 GWLHSGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVVPMKDE 480
           GWLH+GD+G VDDDDE+FIVDRLKELIKYKGFQVAPAELE LLIS+ HI D AV+PMKDE
Sbjct: 421 GWLHTGDMGLVDDDDELFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDE 480

Query: 481 VAGEVPAAFIVRSDSSNIAEDEIKQYISKQVVFYKRINRVFFVDSIPKSSSGKILRRQLR 540
           VAGEVP AFIVRSD SNI EDEIKQYISKQVVFYKRINRVFFVDSIPKS SGKILRRQLR
Sbjct: 481 VAGEVPVAFIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLR 540

Query: 541 ALLAASIPN 548
           ALL ASIPN
Sbjct: 541 ALLPASIPN 545

BLAST of MS002251 vs. ExPASy TrEMBL
Match: A0A5D3CN41 (4-coumarate--CoA ligase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00670 PE=4 SV=1)

HSP 1 Score: 827.4 bits (2136), Expect = 3.5e-236
Identity = 433/551 (78.58%), Postives = 477/551 (86.57%), Query Frame = 0

Query: 1   MAAAD-PPPEFIFRSKLPDITIPNHLPLHTYCFEKLPEFRDRPCLINAATGETHTYGEVH 60
           MAAAD   PEF+FRSKLP+I I  HLPLHTYCFE + EF+ RPCLINAATG T+TYGEV 
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 61  KTARRVAAGLHRMGIRKGDVIMLLLQNSPQFVLAFLGASFLGAVATMANPFFTATEVAKQ 120
            T+RRVAAGLH++GI KGDVIMLLLQN+P+FVLAFL AS+LGA ATMANPFF   E+AKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 121 AGSAGAKLIITQAAFAAKVKNLTCHENDVIIKVIYIDQPPPDDDGGRHFSELM----RAA 180
           A S+GAK+IITQ+AFA KVK L+   +++IIKVI+ID   P   GG  FS L+    +  
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLS-QGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEE 180

Query: 181 EEEIPEVKISPDDVVALPYSSGTTGLPKGVMLTHRALVTSVAQQVDGENPHLNVRRDDVV 240
           E E+ +VKISPDDVVALPYSSGTTGLPKGVMLTH+  VTSVAQQVDGENPHL +R DDVV
Sbjct: 181 ELEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVV 240

Query: 241 LCVLPLFHIYSLNCIMMCSLRVGAAILIVHKFDINSLVKFVPKYKVTVAPLVPPIVLAIA 300
           LC+LPLFHIYSLN IMMCSLRVGAAILIV KFDINSL++ VPKYKVT AP+VPPIVLAIA
Sbjct: 241 LCLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIA 300

Query: 301 ESPAVESSDMSSIRMVLSGAAPLGKELEDAVKAKLPQAILGQGYRMTE-GLVFTMSLAFA 360
           +SPAV+  DMSS+RMVLSGAAPLGK LEDA +AKLP  ILGQGY MTE G V TMSLAFA
Sbjct: 301 KSPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFA 360

Query: 361 KERFEVKSGACGTLMRNSEMKIINPQTGVSLPRNQPGEICIRSTQFMKGYLHDEEATKGI 420
           KE F +KSG CGT+MRNSEMKI+ PQTGVSLPRN+ GEICIRS Q MKGYL+DEEATK I
Sbjct: 361 KEGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAI 420

Query: 421 IDKNGWLHSGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVVP 480
           IDK+GWLH+GDIG+VDDDDE+FIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAV+P
Sbjct: 421 IDKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIP 480

Query: 481 MKDEVAGEVPAAFIVRSDSSNIAEDEIKQYISKQVVFYKRINRVFFVDSIPKSSSGKILR 540
           M DEVAGEVP  FIVR D SNI EDEIKQYISKQVVFYKRINRVFFVDSIPKS SGKILR
Sbjct: 481 MNDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILR 540

Query: 541 RQLRALLAASI 546
           RQLRALLAASI
Sbjct: 541 RQLRALLAASI 550

BLAST of MS002251 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 695.3 bits (1793), Expect = 4.0e-200
Identity = 359/543 (66.11%), Postives = 434/543 (79.93%), Query Frame = 0

Query: 9   EFIFRSKLPDITIPNHLPLHTYCFEKLPEFRDRPCLINAATGETHTYGEVHKTARRVAAG 68
           + IFRS+LPDI IPNHLPLH Y FE + EF  +PCLIN  TGE +TY +VH T+R++AAG
Sbjct: 20  DVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLAAG 79

Query: 69  LHRMGIRKGDVIMLLLQNSPQFVLAFLGASFLGAVATMANPFFTATEVAKQAGSAGAKLI 128
           LH +G+++ DV+M+LL NSP+ VL FL ASF+GA+ T ANPFFT  E++KQA ++ AKLI
Sbjct: 80  LHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAKLI 139

Query: 129 ITQAAFAAKVKNLTCHENDVIIKVIYIDQPPPDDDGGRHFSELMRAAE---EEIPEVKIS 188
           +TQ+ +  K+KNL   +ND ++ V       P++     FSEL ++ E   + IPE KIS
Sbjct: 140 VTQSRYVDKIKNL---QNDGVLIVTTDSDAIPEN--CLRFSELTQSEEPRVDSIPE-KIS 199

Query: 189 PDDVVALPYSSGTTGLPKGVMLTHRALVTSVAQQVDGENPHLNVRRDDVVLCVLPLFHIY 248
           P+DVVALP+SSGTTGLPKGVMLTH+ LVTSVAQQVDGENP+L   RDDV+LCVLP+FHIY
Sbjct: 200 PEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIY 259

Query: 249 SLNCIMMCSLRVGAAILIVHKFDINSLVKFVPKYKVTVAPLVPPIVLAIAESPAVESSDM 308
           +LN IM+CSLRVGA ILI+ KF+I  L++ + + KVTVA +VPPIVLAIA+SP  E  D+
Sbjct: 260 ALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDL 319

Query: 309 SSIRMVLSGAAPLGKELEDAVKAKLPQAILGQGYRMTE-GLVFTMSLAFAKERFEVKSGA 368
           SS+RMV SGAAPLGKELEDA+ AK P A LGQGY MTE G V  MSL FAKE F VKSGA
Sbjct: 320 SSVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGA 379

Query: 369 CGTLMRNSEMKIINPQTGVSLPRNQPGEICIRSTQFMKGYLHDEEATKGIIDKNGWLHSG 428
           CGT++RN+EMKI++P TG SLPRN+PGEICIR  Q MKGYL+D  AT   IDK+GWLH+G
Sbjct: 380 CGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTG 439

Query: 429 DIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVVPMKDEVAGEVP 488
           D+GF+DDDDE+FIVDRLKELIKYKGFQVAPAELE+LLI H  I D AVV MK+E AGEVP
Sbjct: 440 DVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVP 499

Query: 489 AAFIVRSDSSNIAEDEIKQYISKQVVFYKRINRVFFVDSIPKSSSGKILRRQLRALLAAS 548
            AF+VRS  SNI+EDEIKQ++SKQVVFYKRIN+VFF DSIPK+ SGKILR+ LRA LA  
Sbjct: 500 VAFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANG 556

BLAST of MS002251 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 681.0 bits (1756), Expect = 7.8e-196
Identity = 351/538 (65.24%), Postives = 424/538 (78.81%), Query Frame = 0

Query: 9   EFIFRSKLPDITIPNHLPLHTYCFEKLPEFRDRPCLINAATGETHTYGEVHKTARRVAAG 68
           + IFRSKLPDI IPNHL LH Y F+ + EF  +PCLIN  TG  +TY +VH  +R++AA 
Sbjct: 22  DVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAAN 81

Query: 69  LHRMGIRKGDVIMLLLQNSPQFVLAFLGASFLGAVATMANPFFTATEVAKQAGSAGAKLI 128
            H++G+ + DV+MLLL N P+FVL+FL ASF GA AT ANPFFT  E+AKQA ++  KLI
Sbjct: 82  FHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLI 141

Query: 129 ITQAAFAAKVKNLTCHENDVIIKVIYIDQPPPDDDGGRHFSELMRA---AEEEIPEVKIS 188
           IT+A +  K+K L  +++ V+I  I  ++  P  +G   F+EL ++   A E I  V+IS
Sbjct: 142 ITEARYVDKIKPLQ-NDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEIS 201

Query: 189 PDDVVALPYSSGTTGLPKGVMLTHRALVTSVAQQVDGENPHLNVRRDDVVLCVLPLFHIY 248
           PDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENP+L    DDV+LCVLP+FHIY
Sbjct: 202 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIY 261

Query: 249 SLNCIMMCSLRVGAAILIVHKFDINSLVKFVPKYKVTVAPLVPPIVLAIAESPAVESSDM 308
           +LN IM+C LRVGAAILI+ KF+IN L++ + + KVTVAP+VPPIVLAIA+S   E  D+
Sbjct: 262 ALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYDL 321

Query: 309 SSIRMVLSGAAPLGKELEDAVKAKLPQAILGQGYRMTE-GLVFTMSLAFAKERFEVKSGA 368
           SSIR+V SGAAPLGKELEDAV AK P A LGQGY MTE G V  MSL FAKE F VKSGA
Sbjct: 322 SSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGA 381

Query: 369 CGTLMRNSEMKIINPQTGVSLPRNQPGEICIRSTQFMKGYLHDEEATKGIIDKNGWLHSG 428
           CGT++RN+EMKI++P TG SL RNQPGEICIR  Q MKGYL++  AT   IDK+GWLH+G
Sbjct: 382 CGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHTG 441

Query: 429 DIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVVPMKDEVAGEVP 488
           DIG +DDDDE+FIVDRLKELIKYKGFQVAPAELEALLI H  I D AVV MK+E AGEVP
Sbjct: 442 DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEVP 501

Query: 489 AAFIVRSDSSNIAEDEIKQYISKQVVFYKRINRVFFVDSIPKSSSGKILRRQLRALLA 543
            AF+V+S  S ++ED++KQ++SKQVVFYKRIN+VFF +SIPK+ SGKILR+ LRA LA
Sbjct: 502 VAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKLA 558

BLAST of MS002251 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 654.8 bits (1688), Expect = 6.0e-188
Identity = 331/539 (61.41%), Postives = 432/539 (80.15%), Query Frame = 0

Query: 6   PPPEFIFRSKLPDITIPNHLPLHTYCFEKLPEFRDRPCLINAATGETHTYGEVHKTARRV 65
           PP   IFRSKLPDI IPNHLPLHTYCFEKL    D+PCLI  +TG+++TYGE H   RRV
Sbjct: 29  PPTPRIFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRV 88

Query: 66  AAGLHRMGIRKGDVIMLLLQNSPQFVLAFLGASFLGAVATMANPFFTATEVAKQAGSAGA 125
           A+GL+++GIRKGDVIM+LLQNS +FV +F+GAS +GAV+T ANPF+T+ E+ KQ  S+GA
Sbjct: 89  ASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGA 148

Query: 126 KLIITQAAFAAKVKNLTCHENDVIIKVIYIDQPPPDDDGGRHFSELMRAAEEE--IPEVK 185
           KLIIT + +  K+KNL   EN   + +I  D+P P++     FS L+   E       V 
Sbjct: 149 KLIITHSQYVDKLKNL--GEN---LTLITTDEPTPEN--CLPFSTLITDDETNPFQETVD 208

Query: 186 ISPDDVVALPYSSGTTGLPKGVMLTHRALVTSVAQQVDGENPHLNVRRDDVVLCVLPLFH 245
           I  DD  ALP+SSGTTGLPKGV+LTH++L+TSVAQQVDG+NP+L ++ +DV+LCVLPLFH
Sbjct: 209 IGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFH 268

Query: 246 IYSLNCIMMCSLRVGAAILIVHKFDINSLVKFVPKYKVTVAPLVPPIVLAIAESPAVESS 305
           IYSLN +++ SLR GA +L++HKF+I +L+  + +++VT+A LVPP+V+A+A++P V S 
Sbjct: 269 IYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSY 328

Query: 306 DMSSIRMVLSGAAPLGKELEDAVKAKLPQAILGQGYRMTE-GLVFTMSLAFAKERFEVKS 365
           D+SS+R VLSGAAPLGKEL+D+++ +LPQAILGQGY MTE G V +MSL FAKE    KS
Sbjct: 329 DLSSVRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKS 388

Query: 366 GACGTLMRNSEMKIINPQTGVSLPRNQPGEICIRSTQFMKGYLHDEEATKGIIDKNGWLH 425
           G+CGT++RN+E+K+++ +T +SL  NQPGEICIR  Q MK YL+D EAT   ID+ GWLH
Sbjct: 389 GSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLH 448

Query: 426 SGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVVPMKDEVAGE 485
           +GDIG+VD+DDE+FIVDRLKE+IK+KGFQV PAELE+LLI+H  IADAAVVP  DEVAGE
Sbjct: 449 TGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGE 508

Query: 486 VPAAFIVRSDSSNIAEDEIKQYISKQVVFYKRINRVFFVDSIPKSSSGKILRRQLRALL 542
           VP AF+VRS+ ++I E+++K+Y++KQVVFYKR+++VFFV SIPKS SGKILR+ L+A L
Sbjct: 509 VPVAFVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of MS002251 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 636.3 bits (1640), Expect = 2.2e-182
Identity = 323/546 (59.16%), Postives = 413/546 (75.64%), Query Frame = 0

Query: 5   DPPPEFIFRSKLPDITIPNHLPLHTYCFEKLPEFRD----RPCLINAATGETHTYGEVHK 64
           +P  +FIFRSKLPDI IPNHLPL  Y F++     D      C+I+ ATG   TY +V  
Sbjct: 22  EPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADVQT 81

Query: 65  TARRVAAGLHRMGIRKGDVIMLLLQNSPQFVLAFLGASFLGAVATMANPFFTATEVAKQA 124
             RR+AAG+HR+GIR GDV+MLLL NSP+F L+FL  ++LGAV+T ANPF+T  E+AKQA
Sbjct: 82  NMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAKQA 141

Query: 125 GSAGAKLIITQAAFAAKVKNLTCHENDVIIKVIYIDQ-----PPPDDDGGRHFSELMRAA 184
            ++ AK+IIT+     K+ NL   +ND ++ V   D          DDG   F+EL +A 
Sbjct: 142 KASAAKMIITKKCLVDKLTNL---KNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQAD 201

Query: 185 EEEIPEVKISPDDVVALPYSSGTTGLPKGVMLTHRALVTSVAQQVDGENPHLNVRRDDVV 244
           E E+ + KISP+D VA+PYSSGTTGLPKGVM+TH+ LVTS+AQ+VDGENP+LN   +DV+
Sbjct: 202 ETELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVI 261

Query: 245 LCVLPLFHIYSLNCIMMCSLRVGAAILIVHKFDINSLVKFVPKYKVTVAPLVPPIVLAIA 304
           LC LP+FHIY+L+ +M+ ++R GAA+LIV +F++N +++ + +YKVTV P+ PP+VLA  
Sbjct: 262 LCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFI 321

Query: 305 ESPAVESSDMSSIRMVLSGAAPLGKELEDAVKAKLPQAILGQGYRMTEGLVFTMSLAFAK 364
           +SP  E  D+SS+R++LSGAA L KELEDAV+ K P AI GQGY MTE      SLAFAK
Sbjct: 322 KSPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTVAKSLAFAK 381

Query: 365 ERFEVKSGACGTLMRNSEMKIINPQTGVSLPRNQPGEICIRSTQFMKGYLHDEEATKGII 424
             F+ KSGACGT++RN+EMK+++ +TG+SLPRN+ GEIC+R  Q MKGYL+D EAT   I
Sbjct: 382 NPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTI 441

Query: 425 DKNGWLHSGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVVPM 484
           DK+GWLH+GDIGFVDDDDE+FIVDRLKELIK+KG+QVAPAELEALLISH  I DAAVV M
Sbjct: 442 DKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAM 501

Query: 485 KDEVAGEVPAAFIVRSDSSNIAEDEIKQYISKQVVFYKRINRVFFVDSIPKSSSGKILRR 542
           KDEVA EVP AF+ RS  S + ED++K Y++KQVV YKRI  VFF++ IPK+ SGKILR+
Sbjct: 502 KDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRK 561

BLAST of MS002251 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 632.5 bits (1630), Expect = 3.2e-181
Identity = 326/505 (64.55%), Postives = 395/505 (78.22%), Query Frame = 0

Query: 9   EFIFRSKLPDITIPNHLPLHTYCFEKLPEFRDRPCLINAATGETHTYGEVHKTARRVAAG 68
           + IFRSKLPDI IPNHL LH Y F+ + EF  +PCLIN  TG  +TY +VH  +R++AA 
Sbjct: 22  DVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAAN 81

Query: 69  LHRMGIRKGDVIMLLLQNSPQFVLAFLGASFLGAVATMANPFFTATEVAKQAGSAGAKLI 128
            H++G+ + DV+MLLL N P+FVL+FL ASF GA AT ANPFFT  E+AKQA ++  KLI
Sbjct: 82  FHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLI 141

Query: 129 ITQAAFAAKVKNLTCHENDVIIKVIYIDQPPPDDDGGRHFSELMRA---AEEEIPEVKIS 188
           IT+A +  K+K L  +++ V+I  I  ++  P  +G   F+EL ++   A E I  V+IS
Sbjct: 142 ITEARYVDKIKPLQ-NDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEIS 201

Query: 189 PDDVVALPYSSGTTGLPKGVMLTHRALVTSVAQQVDGENPHLNVRRDDVVLCVLPLFHIY 248
           PDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENP+L    DDV+LCVLP+FHIY
Sbjct: 202 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIY 261

Query: 249 SLNCIMMCSLRVGAAILIVHKFDINSLVKFVPKYKVTVAPLVPPIVLAIAESPAVESSDM 308
           +LN IM+C LRVGAAILI+ KF+IN L++ + + KVTVAP+VPPIVLAIA+S   E  D+
Sbjct: 262 ALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYDL 321

Query: 309 SSIRMVLSGAAPLGKELEDAVKAKLPQAILGQGYRMTE-GLVFTMSLAFAKERFEVKSGA 368
           SSIR+V SGAAPLGKELEDAV AK P A LGQGY MTE G V  MSL FAKE F VKSGA
Sbjct: 322 SSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGA 381

Query: 369 CGTLMRNSEMKIINPQTGVSLPRNQPGEICIRSTQFMKGYLHDEEATKGIIDKNGWLHSG 428
           CGT++RN+EMKI++P TG SL RNQPGEICIR  Q MKGYL++  AT   IDK+GWLH+G
Sbjct: 382 CGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHTG 441

Query: 429 DIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVVPMKDEVAGEVP 488
           DIG +DDDDE+FIVDRLKELIKYKGFQVAPAELEALLI H  I D AVV MK+E AGEVP
Sbjct: 442 DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEVP 501

Query: 489 AAFIVRSDSSNIAEDEIKQYISKQV 510
            AF+V+S  S ++ED++KQ++SKQV
Sbjct: 502 VAFVVKSKDSELSEDDVKQFVSKQV 525