MS002224 (gene) Bitter gourd (TR) v1

Overview


Name	MS002224
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Translocase of chloroplast
Location	scaffold30: 3345551 .. 3347628 (-)
RNA-Seq Expression	MS002224
Synteny	MS002224

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTCCCAGGTTATCCGCGAGTGGATTGGGATTAACACCTTTGCAATGGCTACTCAAGACAAATTACTTGAACTGTTGGGTAGACTGAAACAGGAGGTTCTATATTCATTTTTATCCATACATCGTGGTCACAAAATCTATATTATGTGATCTATTTTTTCATGCAAGCTTTATTTGCACTGCATTTTTCACAATCTCTTAGGACTCTTTGCCTCTTGATATATTTGTACAGAATGTCAGCACCTTGACGATACTTGTAATGGGAAAAGGTGGTGTTGGAAAGTCATCCACTGTGAATTCCATTATTGGTGAAAGAGCAGTATCTGTTAGTCCTTTTCAGGTACTCATTACTTATTCGCTTTTGCTGTATTTTGGTTCATTCAAGTGTACAGAAGGTGCATGATTTTATTTAAGAAATTAAATGTTTTATAGTCAGAAGTACCAAGGCCTGTGATGGTTTCTCGCTCAAGGGCTGGATTTACGTTGAACGTCATTGATACACCCGGCATTATAGAAGGAGGTTATATCAATGACCAGGCACTTGAGATCATAAAACGGTAAGTTCTAGTTTTTTACTTCAGATTAATTTTTTACAAGATAATTCAATTAATTCATTATCCTTACTTGTATATCTTTATCCTTCTTTTTTGGATCTTTTTGGGGGTTGATGAGCAGCTTTCTTTTGAATAAGACCATAGATATTCTACTATATGTGGACCGCTTGGATGCATATAGAGTTGATAACTTGGAAAAGCAGGTTATTAAGGCCATAACAGACAGCTTTGGTAAAGCAATATGGAATAGGGCTTTGGTCGTCCTCACTCATGCTCAATTTTCCCCACCAGATGGATTACCCTATGATGAATTTTTCAACAGAAGATCAGAAGCTCTGCTAAAGACAGTTCGATCGGGTGCCTCTTTCAACAAAAATGATATTCAGGTGGCTTTTGACATTTTTATTATCCGTTTTCAGCAAATTTTCAGTTCCATTTTGTTTTTCGTGCAGTTTTTAGTCCTACGTCTATGTGAGCAGATTTTTAATTGATGATACTCATTTCATTACATTAGATCTTTCCTATAAAAAAAATTGGAAAGGATCGTGAAAAAGAAGCAAAAACATGAAGAATTTAAAATTCGAGTTGACAGAGAGGACTTTTACTCGCTCGTGTAAATAGAATTGCATGACGAAGGTCACAATTTTTTGACAGTACTTGTTAATGCCATTTCTGGGGCGGAGGGGATTGAATAGAGTGTAAAGTGAACTATTTAGTCGGCGGCATGTTTAAAACGTGAAAATGTTATTTGTGATTGATTACATTTTTCCTCACTAGTACTGTATTGTCTTCTGATTAGCGTATGAGTTATATTAATGTTCATAACCATGGCATTTGATGTCTTTTGATCTTTCTTTCTGTAAAATCTCTTAGAATTCTGGCAAGAGATGAAATCCATGTTGTTCTTTTGTTCAACAAAGCTTACGTTTATTATTTTAGATCAAATGTTTTTGGTGCTCATTGACATGATAAGAATATTTATCTAATTCCCTATACCTTTCAGGGGTCAAATATTCCAGTCGTCTTAGTTGAGAACAGCGGACGGTGCAACAAGAACGAGAAAGACGAAAAGGTTCTACCTCTGTCTTTCTTTCTTCTGTAGAAATCCTCATTAGAATGTTTTCAATTGAATGATTTGACTGTTGAATTTTACAATAGGTTCTTCCAAACGGAACTGCTTGGATTCCTCACTTGGTTGAGACCATCACCACAGTTGTGTTGAATGGAAGCAAGTCTATTTTTGTTGATAAGAAGTTGATTGAGGGCCCAAATCCCAACCAGAGGGGGAAGTTGCTGATTCCCTTTATTTTTGCACTTCAAGTATGCATCTTCAACTTAAAGCTTCTATAAAATTTTCTTCTCTTACAAGGGACTGAGTTAATGTGTTTGCTTCTCCTTTTGGCTTATTGATAGTATCTCTTCGTTGTTAAACCTATCGAACGTGCAATAAGAAGCGATATCTCAAAAGAGAGTAGACCATCGTGGGAGACGCGTGATACAAGCTTCACCAGCCGCAAGTAT

mRNA sequence

ATGGCGTCCCAGGTTATCCGCGAGTGGATTGGGATTAACACCTTTGCAATGGCTACTCAAGACAAATTACTTGAACTGTTGGGTAGACTGAAACAGGAGAATGTCAGCACCTTGACGATACTTGTAATGGGAAAAGGTGGTGTTGGAAAGTCATCCACTGTGAATTCCATTATTGGTGAAAGAGCAGTATCTGTTAGTCCTTTTCAGTCAGAAGTACCAAGGCCTGTGATGGTTTCTCGCTCAAGGGCTGGATTTACGTTGAACGTCATTGATACACCCGGCATTATAGAAGGAGGTTATATCAATGACCAGGCACTTGAGATCATAAAACGCTTTCTTTTGAATAAGACCATAGATATTCTACTATATGTGGACCGCTTGGATGCATATAGAGTTGATAACTTGGAAAAGCAGGTTATTAAGGCCATAACAGACAGCTTTGGTAAAGCAATATGGAATAGGGCTTTGGTCGTCCTCACTCATGCTCAATTTTCCCCACCAGATGGATTACCCTATGATGAATTTTTCAACAGAAGATCAGAAGCTCTGCTAAAGACAGTTCGATCGGGTGCCTCTTTCAACAAAAATGATATTCAGGGGTCAAATATTCCAGTCGTCTTAGTTGAGAACAGCGGACGGTGCAACAAGAACGAGAAAGACGAAAAGGTTCTTCCAAACGGAACTGCTTGGATTCCTCACTTGGTTGAGACCATCACCACAGTTGTGTTGAATGGAAGCAAGTCTATTTTTGTTGATAAGAAGTTGATTGAGGGCCCAAATCCCAACCAGAGGGGGAAGTTGCTGATTCCCTTTATTTTTGCACTTCAATATCTCTTCGTTGTTAAACCTATCGAACGTGCAATAAGAAGCGATATCTCAAAAGAGAGTAGACCATCGTGGGAGACGCGTGATACAAGCTTCACCAGCCGCAAGTAT

Coding sequence (CDS)

ATGGCGTCCCAGGTTATCCGCGAGTGGATTGGGATTAACACCTTTGCAATGGCTACTCAAGACAAATTACTTGAACTGTTGGGTAGACTGAAACAGGAGAATGTCAGCACCTTGACGATACTTGTAATGGGAAAAGGTGGTGTTGGAAAGTCATCCACTGTGAATTCCATTATTGGTGAAAGAGCAGTATCTGTTAGTCCTTTTCAGTCAGAAGTACCAAGGCCTGTGATGGTTTCTCGCTCAAGGGCTGGATTTACGTTGAACGTCATTGATACACCCGGCATTATAGAAGGAGGTTATATCAATGACCAGGCACTTGAGATCATAAAACGCTTTCTTTTGAATAAGACCATAGATATTCTACTATATGTGGACCGCTTGGATGCATATAGAGTTGATAACTTGGAAAAGCAGGTTATTAAGGCCATAACAGACAGCTTTGGTAAAGCAATATGGAATAGGGCTTTGGTCGTCCTCACTCATGCTCAATTTTCCCCACCAGATGGATTACCCTATGATGAATTTTTCAACAGAAGATCAGAAGCTCTGCTAAAGACAGTTCGATCGGGTGCCTCTTTCAACAAAAATGATATTCAGGGGTCAAATATTCCAGTCGTCTTAGTTGAGAACAGCGGACGGTGCAACAAGAACGAGAAAGACGAAAAGGTTCTTCCAAACGGAACTGCTTGGATTCCTCACTTGGTTGAGACCATCACCACAGTTGTGTTGAATGGAAGCAAGTCTATTTTTGTTGATAAGAAGTTGATTGAGGGCCCAAATCCCAACCAGAGGGGGAAGTTGCTGATTCCCTTTATTTTTGCACTTCAATATCTCTTCGTTGTTAAACCTATCGAACGTGCAATAAGAAGCGATATCTCAAAAGAGAGTAGACCATCGTGGGAGACGCGTGATACAAGCTTCACCAGCCGCAAGTAT

Protein sequence

MASQVIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGERAVSVSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDILLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRSEALLKTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITTVVLNGSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRPSWETRDTSFTSRKY

Homology

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BLAST of MS002224 vs. NCBI nr
Match: XP_022135187.1 (uncharacterized protein LOC111007215 [Momordica charantia] >XP_022135188.1 uncharacterized protein LOC111007215 [Momordica charantia])

HSP 1 Score: 613.6 bits (1581), Expect = 9.4e-172
Identity = 312/312 (100.00%), Postives = 312/312 (100.00%), Query Frame = 0


Query: 1   MASQVIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGE 60
           MASQVIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGE
Sbjct: 1   MASQVIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGE 60

Query: 61  RAVSVSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDI 120
           RAVSVSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDI
Sbjct: 61  RAVSVSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDI 120

Query: 121 LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRS 180
           LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRS
Sbjct: 121 LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRS 180

Query: 181 EALLKTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITT 240
           EALLKTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITT
Sbjct: 181 EALLKTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITT 240

Query: 241 VVLNGSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRPSW 300
           VVLNGSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRPSW
Sbjct: 241 VVLNGSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRPSW 300

Query: 301 ETRDTSFTSRKY 313
           ETRDTSFTSRKY
Sbjct: 301 ETRDTSFTSRKY 312

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BLAST of MS002224 vs. NCBI nr
Match: XP_038876609.1 (translocase of chloroplast 34 [Benincasa hispida])

HSP 1 Score: 578.6 bits (1490), Expect = 3.3e-161
Identity = 295/312 (94.55%), Postives = 303/312 (97.12%), Query Frame = 0


Query: 1   MASQVIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGE 60
           MASQV+REWIGIN FAMATQDKLLELLG+LKQ NV+TLTILVMGKGGVGKSSTVNS+IGE
Sbjct: 1   MASQVVREWIGINKFAMATQDKLLELLGKLKQ-NVNTLTILVMGKGGVGKSSTVNSLIGE 60

Query: 61  RAVSVSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDI 120
           RAVSVSPFQSEVPRPVMVSRSRAGFTLN+IDTPGIIEGGYINDQALEIIKRFLLNKTIDI
Sbjct: 61  RAVSVSPFQSEVPRPVMVSRSRAGFTLNIIDTPGIIEGGYINDQALEIIKRFLLNKTIDI 120

Query: 121 LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRS 180
           LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEF +RRS
Sbjct: 121 LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFVSRRS 180

Query: 181 EALLKTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITT 240
           EALLKTVRSGAS NKNDIQGS IPVVLVENSGRCN+NEKDEKVLPNG AWIPHLVETITT
Sbjct: 181 EALLKTVRSGASINKNDIQGSKIPVVLVENSGRCNQNEKDEKVLPNGIAWIPHLVETITT 240

Query: 241 VVLNGSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRPSW 300
           VVLNGSKSI VDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRPSW
Sbjct: 241 VVLNGSKSICVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRPSW 300

Query: 301 ETRDTSFTSRKY 313
           E RDTS +SRKY
Sbjct: 301 EMRDTSLSSRKY 311

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BLAST of MS002224 vs. NCBI nr
Match: XP_016900644.1 (PREDICTED: translocase of chloroplast 34-like [Cucumis melo] >XP_016900645.1 PREDICTED: translocase of chloroplast 34-like [Cucumis melo] >ADN33942.1 chloroplast outer envelope protein 34 [Cucumis melo subsp. melo])

HSP 1 Score: 553.9 bits (1426), Expect = 8.8e-154
Identity = 278/312 (89.10%), Postives = 295/312 (94.55%), Query Frame = 0


Query: 1   MASQVIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGE 60
           MASQVIREW+GIN FAMATQ KLLEL+G+LKQENV+TLTILVMGKGGVGKSSTVNSIIGE
Sbjct: 1   MASQVIREWVGINNFAMATQAKLLELMGKLKQENVNTLTILVMGKGGVGKSSTVNSIIGE 60

Query: 61  RAVSVSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDI 120
           R VSVSPFQSEVPRPVMVSRSRAGFTLN+IDTPGIIEGGYINDQALEIIKRFLLNKTIDI
Sbjct: 61  RVVSVSPFQSEVPRPVMVSRSRAGFTLNIIDTPGIIEGGYINDQALEIIKRFLLNKTIDI 120

Query: 121 LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRS 180
           LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIW+RAL+VLTHAQFSPPDGLPYDEF +RRS
Sbjct: 121 LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWSRALIVLTHAQFSPPDGLPYDEFVSRRS 180

Query: 181 EALLKTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITT 240
           EAL+KTVR GASF K +IQG NIPVVLVENSGRC+KNEKDEKVLPNG AWIPHLVETITT
Sbjct: 181 EALVKTVRFGASFRKVEIQGLNIPVVLVENSGRCSKNEKDEKVLPNGIAWIPHLVETITT 240

Query: 241 VVLNGSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRPSW 300
           VVL GSKSIFVDK LIEGPNPNQRGKLLIP IFALQY FVVKPI+RAIR DI+++ RPSW
Sbjct: 241 VVLKGSKSIFVDKTLIEGPNPNQRGKLLIPLIFALQYFFVVKPIKRAIRRDIARQIRPSW 300

Query: 301 ETRDTSFTSRKY 313
           E RD SF++RKY
Sbjct: 301 ELRDMSFSTRKY 312

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BLAST of MS002224 vs. NCBI nr
Match: TYK11416.1 (translocase of chloroplast 34-like [Cucumis melo var. makuwa])

HSP 1 Score: 553.9 bits (1426), Expect = 8.8e-154
Identity = 278/312 (89.10%), Postives = 295/312 (94.55%), Query Frame = 0


Query: 1   MASQVIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGE 60
           MASQVIREW+GIN FAMATQ KLLEL+G+LKQENV+TLTILVMGKGGVGKSSTVNSIIGE
Sbjct: 8   MASQVIREWVGINNFAMATQAKLLELMGKLKQENVNTLTILVMGKGGVGKSSTVNSIIGE 67

Query: 61  RAVSVSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDI 120
           R VSVSPFQSEVPRPVMVSRSRAGFTLN+IDTPGIIEGGYINDQALEIIKRFLLNKTIDI
Sbjct: 68  RVVSVSPFQSEVPRPVMVSRSRAGFTLNIIDTPGIIEGGYINDQALEIIKRFLLNKTIDI 127

Query: 121 LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRS 180
           LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIW+RAL+VLTHAQFSPPDGLPYDEF +RRS
Sbjct: 128 LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWSRALIVLTHAQFSPPDGLPYDEFVSRRS 187

Query: 181 EALLKTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITT 240
           EAL+KTVR GASF K +IQG NIPVVLVENSGRC+KNEKDEKVLPNG AWIPHLVETITT
Sbjct: 188 EALVKTVRFGASFRKVEIQGLNIPVVLVENSGRCSKNEKDEKVLPNGIAWIPHLVETITT 247

Query: 241 VVLNGSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRPSW 300
           VVL GSKSIFVDK LIEGPNPNQRGKLLIP IFALQY FVVKPI+RAIR DI+++ RPSW
Sbjct: 248 VVLKGSKSIFVDKTLIEGPNPNQRGKLLIPLIFALQYFFVVKPIKRAIRRDIARQIRPSW 307

Query: 301 ETRDTSFTSRKY 313
           E RD SF++RKY
Sbjct: 308 ELRDMSFSTRKY 319

[Close]

BLAST of MS002224 vs. NCBI nr
Match: KAA0052960.1 (translocase of chloroplast 34-like [Cucumis melo var. makuwa])

HSP 1 Score: 552.7 bits (1423), Expect = 2.0e-153
Identity = 277/312 (88.78%), Postives = 295/312 (94.55%), Query Frame = 0


Query: 1   MASQVIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGE 60
           MASQVIREW+GIN FAMATQ KLLEL+G+LKQENV+TLTILVMGKGGVGKSSTVNSIIGE
Sbjct: 8   MASQVIREWVGINNFAMATQAKLLELMGKLKQENVNTLTILVMGKGGVGKSSTVNSIIGE 67

Query: 61  RAVSVSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDI 120
           R VSVSPFQSEVPRPVMVSRSRAGFTLN+IDTPGIIEGGYINDQALEIIKRFLLNKTIDI
Sbjct: 68  RVVSVSPFQSEVPRPVMVSRSRAGFTLNIIDTPGIIEGGYINDQALEIIKRFLLNKTIDI 127

Query: 121 LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRS 180
           LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIW+RAL+VLTHAQFSPPDGLPYDEF +RRS
Sbjct: 128 LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWSRALIVLTHAQFSPPDGLPYDEFVSRRS 187

Query: 181 EALLKTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITT 240
           EAL++TVR GASF K +IQG NIPVVLVENSGRC+KNEKDEKVLPNG AWIPHLVETITT
Sbjct: 188 EALVRTVRFGASFRKVEIQGLNIPVVLVENSGRCSKNEKDEKVLPNGIAWIPHLVETITT 247

Query: 241 VVLNGSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRPSW 300
           VVL GSKSIFVDK LIEGPNPNQRGKLLIP IFALQY FVVKPI+RAIR DI+++ RPSW
Sbjct: 248 VVLKGSKSIFVDKTLIEGPNPNQRGKLLIPLIFALQYFFVVKPIKRAIRRDIARQIRPSW 307

Query: 301 ETRDTSFTSRKY 313
           E RD SF++RKY
Sbjct: 308 ELRDMSFSTRKY 319

[Close]

BLAST of MS002224 vs. ExPASy Swiss-Prot
Match: Q41009 (Translocase of chloroplast 34 OS=Pisum sativum OX=3888 GN=TOC34 PE=1 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 3.5e-137
Identity = 242/301 (80.40%), Postives = 268/301 (89.04%), Query Frame = 0


Query: 4   QVIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGERAV 63
           Q +REW GINTFA ATQ KLLELLG LKQE+V++LTILVMGKGGVGKSSTVNSIIGER V
Sbjct: 6   QTVREWSGINTFAPATQTKLLELLGNLKQEDVNSLTILVMGKGGVGKSSTVNSIIGERVV 65

Query: 64  SVSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDILLY 123
           S+SPFQSE PRPVMVSRSRAGFTLN+IDTPG+IEGGYIND AL IIK FLL+KTID+LLY
Sbjct: 66  SISPFQSEGPRPVMVSRSRAGFTLNIIDTPGLIEGGYINDMALNIIKSFLLDKTIDVLLY 125

Query: 124 VDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRSEAL 183
           VDRLDAYRVDNL+K V KAITDSFGK IWN+A+V LTHAQFSPPDGLPYDEFF++RSEAL
Sbjct: 126 VDRLDAYRVDNLDKLVAKAITDSFGKGIWNKAIVALTHAQFSPPDGLPYDEFFSKRSEAL 185

Query: 184 LKTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITTVVL 243
           L+ VRSGAS  K D Q S+IPVVL+ENSGRCNKN+ DEKVLPNG AWIPHLV+TIT V L
Sbjct: 186 LQVVRSGASL-KKDAQASDIPVVLIENSGRCNKNDSDEKVLPNGIAWIPHLVQTITEVAL 245

Query: 244 NGSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRPSWETR 303
           N S+SIFVDK LI+GPNPNQRGKL IP IFALQYLF+ KPIE  IR DI+ E++P+WETR
Sbjct: 246 NKSESIFVDKNLIDGPNPNQRGKLWIPLIFALQYLFLAKPIEALIRRDIATETKPAWETR 305

Query: 304 D 305
           D
Sbjct: 306 D 305

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BLAST of MS002224 vs. ExPASy Swiss-Prot
Match: Q38906 (Translocase of chloroplast 34, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TOC34 PE=1 SV=2)

HSP 1 Score: 443.7 bits (1140), Expect = 1.7e-123
Identity = 208/310 (67.10%), Postives = 262/310 (84.52%), Query Frame = 0


Query: 2   ASQVIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGER 61
           A Q +REWIGI  F  ATQ KLLE+LG+ K+E+VS+LT+LVMGKGGVGKSSTVNS+IGE+
Sbjct: 3   ALQTLREWIGIQQFPPATQSKLLEILGKYKEEDVSSLTVLVMGKGGVGKSSTVNSVIGEK 62

Query: 62  AVSVSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDIL 121
           A +VS FQSE  RP +VSR+R+GFTLN+IDTPG+IEGGY+NDQA+ IIKRFLLN TID+L
Sbjct: 63  AAAVSTFQSEGLRPTLVSRTRSGFTLNIIDTPGLIEGGYVNDQAINIIKRFLLNMTIDVL 122

Query: 122 LYVDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRSE 181
           LYVDRLD YRVD+L++QV+ AITD+FGK IW ++ +VLTHAQFSPPDGL Y+ F ++RS 
Sbjct: 123 LYVDRLDVYRVDDLDRQVVGAITDAFGKEIWKKSALVLTHAQFSPPDGLNYNHFVSKRSN 182

Query: 182 ALLKTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITTV 241
           ALLK +++GA   K D+QG +IPV+LVENSGRC+KNE DEK+LP GT+WIP+L   IT +
Sbjct: 183 ALLKVIQTGAQLKKQDLQGFSIPVILVENSGRCHKNESDEKILPCGTSWIPNLFNKITEI 242

Query: 242 VLNGSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRPSWE 301
             NG+K+I VDKKL+EGPNPN+RGK LIP +FA QYL V+KP+ RAI+SD+S+ES+P+WE
Sbjct: 243 SFNGNKAIHVDKKLVEGPNPNERGKKLIPLMFAFQYLLVMKPLVRAIKSDVSRESKPAWE 302

Query: 302 TRDTSFTSRK 312
            RD+   SR+
Sbjct: 303 LRDSGLASRR 312

[Close]

BLAST of MS002224 vs. ExPASy Swiss-Prot
Match: O23680 (Translocase of chloroplast 33, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TOC33 PE=1 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 1.4e-101
Identity = 176/294 (59.86%), Postives = 232/294 (78.91%), Query Frame = 0


Query: 5   VIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGERAVS 64
           ++REW+G   F  ATQ+KL+E  G+LKQ++++++T+LV+GKGGVGKSSTVNS+IGE+ V 
Sbjct: 4   LVREWVGFQQFPAATQEKLIEFFGKLKQKDMNSMTVLVLGKGGVGKSSTVNSLIGEQVVR 63

Query: 65  VSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDILLYV 124
           VSPFQ+E  RPVMVSR+  GFT+N+IDTPG++E GY+N QALE+IK FL+N+TID+LLYV
Sbjct: 64  VSPFQAEGLRPVMVSRTMGGFTINIIDTPGLVEAGYVNHQALELIKGFLVNRTIDVLLYV 123

Query: 125 DRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRSEALL 184
           DRLD YRVD L+KQV+ AIT +FGK IW + L+VLTHAQFSPPD L Y+ F ++RS++LL
Sbjct: 124 DRLDVYRVDELDKQVVIAITQTFGKEIWCKTLLVLTHAQFSPPDELSYETFSSKRSDSLL 183

Query: 185 KTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITTVVLN 244
           KT+R+G+   K + + S I VV  ENSGRC+KN+KDEK LPNG AWIP+LV+ IT V  N
Sbjct: 184 KTIRAGSKMRKQEFEDSAIAVVYAENSGRCSKNDKDEKALPNGEAWIPNLVKAITDVATN 243

Query: 245 GSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRP 299
             K+I VDKK+++G   + +GK LIP I   QYL +VK I+ AIR+DI    +P
Sbjct: 244 QRKAIHVDKKMVDGSYSDDKGKKLIPLIIGAQYL-IVKMIQGAIRNDIKTSGKP 296

[Close]

BLAST of MS002224 vs. ExPASy Swiss-Prot
Match: A8HYJ1 (Translocase of chloroplast 34 homolog, chloroplastic OS=Chlamydomonas reinhardtii OX=3055 GN=TOC34 PE=1 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 7.0e-37
Identity = 103/285 (36.14%), Postives = 146/285 (51.23%), Query Frame = 0


Query: 9   WIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGERAVSVSPF 68
           W G+N   +  +D +L++L  L+ E    LT+L++GK  VGKSS +NS++GE  V V  F
Sbjct: 66  WAGLN--RLPERDDMLDILNELRAEGRKQLTVLLLGKSSVGKSSLINSLLGEAVVRVQAF 125

Query: 69  Q--------SEVPRPVMVSRSRA-GFTLNVIDTPGIIE---GGYINDQALEIIKRFLLNK 128
           +        + V R V V  S   GF L +IDT G+ +   G  +N  AL  I   +   
Sbjct: 126 KLQADTDITTTVVRQVAVGNSEVDGFRLKLIDTCGLEDPEAGDTVNLGALSKIAEDVRGV 185

Query: 129 TIDILLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQF-SPPDGLPYDEF 188
            ID++LY DRLD YRVD L+K +I AI+ +FG+ IW R +V LTHA     P G  YD F
Sbjct: 186 GIDVVLYCDRLDLYRVDPLDKAIIDAISSTFGRGIWRRTVVALTHANLVQTPPGTDYDSF 245

Query: 189 FNRRSEALLKTVRSGASFNKNDIQGSNIPVVLVENSGRCN-KNEKDEKVLPNGTAWIPHL 248
            N R   +   VR    F        ++PV LVENS  C   +E   +VLP+G  W+  L
Sbjct: 246 VNGRVRLIRGAVRGPLFFR------PSLPVALVENSETCPVSSESGFRVLPDGEPWLVAL 305

Query: 249 VETITTVVLNGSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLF 280
           V  +  +     +      +L     P+ R + L+P   A + LF
Sbjct: 306 VSQLVDMAAARRRPYKYHPRL--SSKPSHRFRWLLPVAIAAEVLF 340

[Close]

BLAST of MS002224 vs. ExPASy Swiss-Prot
Match: Q9LUS2 (Translocase of chloroplast 120, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TOC120 PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 8.0e-33
Identity = 84/233 (36.05%), Postives = 126/233 (54.08%), Query Frame = 0


Query: 12  INTFAMATQDKLLELLGRLKQENVS-TLTILVMGKGGVGKSSTVNSIIGERAVSVSPFQS 71
           +  F+      + E L    Q+ +  + TI+V+GK GVGKS+T+NSI  E  +S   FQ 
Sbjct: 430 VGAFSFDRASAMAEQLEAAAQDPLDFSCTIMVLGKSGVGKSATINSIFDELKISTDAFQV 489

Query: 72  EVPRPVMVSRSRAGFTLNVIDTPGII---EGGYINDQALEIIKRFLLNKTIDILLYVDRL 131
              +   +     G  + VIDTPG++      + N++ L+ ++ F+     DI+LY+DRL
Sbjct: 490 GTKKVQDIEGFVQGIKVRVIDTPGLLPSWSDQHKNEKILKSVRAFIKKSPPDIVLYLDRL 549

Query: 132 DAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDG-----LPYDEFFNRRSEA 191
           D    D+ +  +++ ITD FG +IW  A+V LTHA  +PPDG       YD F  +RS  
Sbjct: 550 DMQSRDSGDMPLLRTITDVFGPSIWFNAIVGLTHAASAPPDGPNGTASSYDMFVTQRSHV 609

Query: 192 LLKTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLV 236
           + + +R  A     D++  N PV LVEN   C  N   ++VLPNG  W PHL+
Sbjct: 610 IQQAIRQAA----GDMRLMN-PVSLVENHSACRTNRAGQRVLPNGQVWKPHLL 657

[Close]

BLAST of MS002224 vs. ExPASy TrEMBL
Match: A0A6J1C1Z3 (Translocase of chloroplast OS=Momordica charantia OX=3673 GN=LOC111007215 PE=3 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 4.5e-172
Identity = 312/312 (100.00%), Postives = 312/312 (100.00%), Query Frame = 0


Query: 1   MASQVIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGE 60
           MASQVIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGE
Sbjct: 1   MASQVIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGE 60

Query: 61  RAVSVSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDI 120
           RAVSVSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDI
Sbjct: 61  RAVSVSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDI 120

Query: 121 LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRS 180
           LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRS
Sbjct: 121 LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRS 180

Query: 181 EALLKTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITT 240
           EALLKTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITT
Sbjct: 181 EALLKTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITT 240

Query: 241 VVLNGSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRPSW 300
           VVLNGSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRPSW
Sbjct: 241 VVLNGSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRPSW 300

Query: 301 ETRDTSFTSRKY 313
           ETRDTSFTSRKY
Sbjct: 301 ETRDTSFTSRKY 312

[Close]

BLAST of MS002224 vs. ExPASy TrEMBL
Match: A0A1S4DXD3 (Translocase of chloroplast OS=Cucumis melo OX=3656 GN=LOC107990978 PE=3 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 4.3e-154
Identity = 278/312 (89.10%), Postives = 295/312 (94.55%), Query Frame = 0


Query: 1   MASQVIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGE 60
           MASQVIREW+GIN FAMATQ KLLEL+G+LKQENV+TLTILVMGKGGVGKSSTVNSIIGE
Sbjct: 1   MASQVIREWVGINNFAMATQAKLLELMGKLKQENVNTLTILVMGKGGVGKSSTVNSIIGE 60

Query: 61  RAVSVSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDI 120
           R VSVSPFQSEVPRPVMVSRSRAGFTLN+IDTPGIIEGGYINDQALEIIKRFLLNKTIDI
Sbjct: 61  RVVSVSPFQSEVPRPVMVSRSRAGFTLNIIDTPGIIEGGYINDQALEIIKRFLLNKTIDI 120

Query: 121 LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRS 180
           LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIW+RAL+VLTHAQFSPPDGLPYDEF +RRS
Sbjct: 121 LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWSRALIVLTHAQFSPPDGLPYDEFVSRRS 180

Query: 181 EALLKTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITT 240
           EAL+KTVR GASF K +IQG NIPVVLVENSGRC+KNEKDEKVLPNG AWIPHLVETITT
Sbjct: 181 EALVKTVRFGASFRKVEIQGLNIPVVLVENSGRCSKNEKDEKVLPNGIAWIPHLVETITT 240

Query: 241 VVLNGSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRPSW 300
           VVL GSKSIFVDK LIEGPNPNQRGKLLIP IFALQY FVVKPI+RAIR DI+++ RPSW
Sbjct: 241 VVLKGSKSIFVDKTLIEGPNPNQRGKLLIPLIFALQYFFVVKPIKRAIRRDIARQIRPSW 300

Query: 301 ETRDTSFTSRKY 313
           E RD SF++RKY
Sbjct: 301 ELRDMSFSTRKY 312

[Close]

BLAST of MS002224 vs. ExPASy TrEMBL
Match: A0A5D3CLX1 (Translocase of chloroplast OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00700 PE=3 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 4.3e-154
Identity = 278/312 (89.10%), Postives = 295/312 (94.55%), Query Frame = 0


Query: 1   MASQVIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGE 60
           MASQVIREW+GIN FAMATQ KLLEL+G+LKQENV+TLTILVMGKGGVGKSSTVNSIIGE
Sbjct: 8   MASQVIREWVGINNFAMATQAKLLELMGKLKQENVNTLTILVMGKGGVGKSSTVNSIIGE 67

Query: 61  RAVSVSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDI 120
           R VSVSPFQSEVPRPVMVSRSRAGFTLN+IDTPGIIEGGYINDQALEIIKRFLLNKTIDI
Sbjct: 68  RVVSVSPFQSEVPRPVMVSRSRAGFTLNIIDTPGIIEGGYINDQALEIIKRFLLNKTIDI 127

Query: 121 LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRS 180
           LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIW+RAL+VLTHAQFSPPDGLPYDEF +RRS
Sbjct: 128 LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWSRALIVLTHAQFSPPDGLPYDEFVSRRS 187

Query: 181 EALLKTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITT 240
           EAL+KTVR GASF K +IQG NIPVVLVENSGRC+KNEKDEKVLPNG AWIPHLVETITT
Sbjct: 188 EALVKTVRFGASFRKVEIQGLNIPVVLVENSGRCSKNEKDEKVLPNGIAWIPHLVETITT 247

Query: 241 VVLNGSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRPSW 300
           VVL GSKSIFVDK LIEGPNPNQRGKLLIP IFALQY FVVKPI+RAIR DI+++ RPSW
Sbjct: 248 VVLKGSKSIFVDKTLIEGPNPNQRGKLLIPLIFALQYFFVVKPIKRAIRRDIARQIRPSW 307

Query: 301 ETRDTSFTSRKY 313
           E RD SF++RKY
Sbjct: 308 ELRDMSFSTRKY 319

[Close]

BLAST of MS002224 vs. ExPASy TrEMBL
Match: E5RDD4 (Translocase of chloroplast OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 4.3e-154
Identity = 278/312 (89.10%), Postives = 295/312 (94.55%), Query Frame = 0


Query: 1   MASQVIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGE 60
           MASQVIREW+GIN FAMATQ KLLEL+G+LKQENV+TLTILVMGKGGVGKSSTVNSIIGE
Sbjct: 1   MASQVIREWVGINNFAMATQAKLLELMGKLKQENVNTLTILVMGKGGVGKSSTVNSIIGE 60

Query: 61  RAVSVSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDI 120
           R VSVSPFQSEVPRPVMVSRSRAGFTLN+IDTPGIIEGGYINDQALEIIKRFLLNKTIDI
Sbjct: 61  RVVSVSPFQSEVPRPVMVSRSRAGFTLNIIDTPGIIEGGYINDQALEIIKRFLLNKTIDI 120

Query: 121 LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRS 180
           LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIW+RAL+VLTHAQFSPPDGLPYDEF +RRS
Sbjct: 121 LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWSRALIVLTHAQFSPPDGLPYDEFVSRRS 180

Query: 181 EALLKTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITT 240
           EAL+KTVR GASF K +IQG NIPVVLVENSGRC+KNEKDEKVLPNG AWIPHLVETITT
Sbjct: 181 EALVKTVRFGASFRKVEIQGLNIPVVLVENSGRCSKNEKDEKVLPNGIAWIPHLVETITT 240

Query: 241 VVLNGSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRPSW 300
           VVL GSKSIFVDK LIEGPNPNQRGKLLIP IFALQY FVVKPI+RAIR DI+++ RPSW
Sbjct: 241 VVLKGSKSIFVDKTLIEGPNPNQRGKLLIPLIFALQYFFVVKPIKRAIRRDIARQIRPSW 300

Query: 301 ETRDTSFTSRKY 313
           E RD SF++RKY
Sbjct: 301 ELRDMSFSTRKY 312

[Close]

BLAST of MS002224 vs. ExPASy TrEMBL
Match: A0A5A7UCN3 (Translocase of chloroplast OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold344G00690 PE=3 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 9.5e-154
Identity = 277/312 (88.78%), Postives = 295/312 (94.55%), Query Frame = 0


Query: 1   MASQVIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGE 60
           MASQVIREW+GIN FAMATQ KLLEL+G+LKQENV+TLTILVMGKGGVGKSSTVNSIIGE
Sbjct: 8   MASQVIREWVGINNFAMATQAKLLELMGKLKQENVNTLTILVMGKGGVGKSSTVNSIIGE 67

Query: 61  RAVSVSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDI 120
           R VSVSPFQSEVPRPVMVSRSRAGFTLN+IDTPGIIEGGYINDQALEIIKRFLLNKTIDI
Sbjct: 68  RVVSVSPFQSEVPRPVMVSRSRAGFTLNIIDTPGIIEGGYINDQALEIIKRFLLNKTIDI 127

Query: 121 LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRS 180
           LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIW+RAL+VLTHAQFSPPDGLPYDEF +RRS
Sbjct: 128 LLYVDRLDAYRVDNLEKQVIKAITDSFGKAIWSRALIVLTHAQFSPPDGLPYDEFVSRRS 187

Query: 181 EALLKTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITT 240
           EAL++TVR GASF K +IQG NIPVVLVENSGRC+KNEKDEKVLPNG AWIPHLVETITT
Sbjct: 188 EALVRTVRFGASFRKVEIQGLNIPVVLVENSGRCSKNEKDEKVLPNGIAWIPHLVETITT 247

Query: 241 VVLNGSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRPSW 300
           VVL GSKSIFVDK LIEGPNPNQRGKLLIP IFALQY FVVKPI+RAIR DI+++ RPSW
Sbjct: 248 VVLKGSKSIFVDKTLIEGPNPNQRGKLLIPLIFALQYFFVVKPIKRAIRRDIARQIRPSW 307

Query: 301 ETRDTSFTSRKY 313
           E RD SF++RKY
Sbjct: 308 ELRDMSFSTRKY 319

[Close]

BLAST of MS002224 vs. TAIR 10
Match: AT5G05000.2 (translocon at the outer envelope membrane of chloroplasts 34 )

HSP 1 Score: 443.7 bits (1140), Expect = 1.2e-124
Identity = 208/310 (67.10%), Postives = 262/310 (84.52%), Query Frame = 0


Query: 2   ASQVIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGER 61
           A Q +REWIGI  F  ATQ KLLE+LG+ K+E+VS+LT+LVMGKGGVGKSSTVNS+IGE+
Sbjct: 3   ALQTLREWIGIQQFPPATQSKLLEILGKYKEEDVSSLTVLVMGKGGVGKSSTVNSVIGEK 62

Query: 62  AVSVSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDIL 121
           A +VS FQSE  RP +VSR+R+GFTLN+IDTPG+IEGGY+NDQA+ IIKRFLLN TID+L
Sbjct: 63  AAAVSTFQSEGLRPTLVSRTRSGFTLNIIDTPGLIEGGYVNDQAINIIKRFLLNMTIDVL 122

Query: 122 LYVDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRSE 181
           LYVDRLD YRVD+L++QV+ AITD+FGK IW ++ +VLTHAQFSPPDGL Y+ F ++RS 
Sbjct: 123 LYVDRLDVYRVDDLDRQVVGAITDAFGKEIWKKSALVLTHAQFSPPDGLNYNHFVSKRSN 182

Query: 182 ALLKTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITTV 241
           ALLK +++GA   K D+QG +IPV+LVENSGRC+KNE DEK+LP GT+WIP+L   IT +
Sbjct: 183 ALLKVIQTGAQLKKQDLQGFSIPVILVENSGRCHKNESDEKILPCGTSWIPNLFNKITEI 242

Query: 242 VLNGSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRPSWE 301
             NG+K+I VDKKL+EGPNPN+RGK LIP +FA QYL V+KP+ RAI+SD+S+ES+P+WE
Sbjct: 243 SFNGNKAIHVDKKLVEGPNPNERGKKLIPLMFAFQYLLVMKPLVRAIKSDVSRESKPAWE 302

Query: 302 TRDTSFTSRK 312
            RD+   SR+
Sbjct: 303 LRDSGLASRR 312

[Close]

BLAST of MS002224 vs. TAIR 10
Match: AT5G05000.3 (translocon at the outer envelope membrane of chloroplasts 34 )

HSP 1 Score: 443.7 bits (1140), Expect = 1.2e-124
Identity = 208/310 (67.10%), Postives = 262/310 (84.52%), Query Frame = 0


Query: 2   ASQVIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGER 61
           A Q +REWIGI  F  ATQ KLLE+LG+ K+E+VS+LT+LVMGKGGVGKSSTVNS+IGE+
Sbjct: 3   ALQTLREWIGIQQFPPATQSKLLEILGKYKEEDVSSLTVLVMGKGGVGKSSTVNSVIGEK 62

Query: 62  AVSVSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDIL 121
           A +VS FQSE  RP +VSR+R+GFTLN+IDTPG+IEGGY+NDQA+ IIKRFLLN TID+L
Sbjct: 63  AAAVSTFQSEGLRPTLVSRTRSGFTLNIIDTPGLIEGGYVNDQAINIIKRFLLNMTIDVL 122

Query: 122 LYVDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRSE 181
           LYVDRLD YRVD+L++QV+ AITD+FGK IW ++ +VLTHAQFSPPDGL Y+ F ++RS 
Sbjct: 123 LYVDRLDVYRVDDLDRQVVGAITDAFGKEIWKKSALVLTHAQFSPPDGLNYNHFVSKRSN 182

Query: 182 ALLKTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITTV 241
           ALLK +++GA   K D+QG +IPV+LVENSGRC+KNE DEK+LP GT+WIP+L   IT +
Sbjct: 183 ALLKVIQTGAQLKKQDLQGFSIPVILVENSGRCHKNESDEKILPCGTSWIPNLFNKITEI 242

Query: 242 VLNGSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRPSWE 301
             NG+K+I VDKKL+EGPNPN+RGK LIP +FA QYL V+KP+ RAI+SD+S+ES+P+WE
Sbjct: 243 SFNGNKAIHVDKKLVEGPNPNERGKKLIPLMFAFQYLLVMKPLVRAIKSDVSRESKPAWE 302

Query: 302 TRDTSFTSRK 312
            RD+   SR+
Sbjct: 303 LRDSGLASRR 312

[Close]

BLAST of MS002224 vs. TAIR 10
Match: AT5G05000.1 (translocon at the outer envelope membrane of chloroplasts 34 )

HSP 1 Score: 443.7 bits (1140), Expect = 1.2e-124
Identity = 208/310 (67.10%), Postives = 262/310 (84.52%), Query Frame = 0


Query: 2   ASQVIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGER 61
           A Q +REWIGI  F  ATQ KLLE+LG+ K+E+VS+LT+LVMGKGGVGKSSTVNS+IGE+
Sbjct: 3   ALQTLREWIGIQQFPPATQSKLLEILGKYKEEDVSSLTVLVMGKGGVGKSSTVNSVIGEK 62

Query: 62  AVSVSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDIL 121
           A +VS FQSE  RP +VSR+R+GFTLN+IDTPG+IEGGY+NDQA+ IIKRFLLN TID+L
Sbjct: 63  AAAVSTFQSEGLRPTLVSRTRSGFTLNIIDTPGLIEGGYVNDQAINIIKRFLLNMTIDVL 122

Query: 122 LYVDRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRSE 181
           LYVDRLD YRVD+L++QV+ AITD+FGK IW ++ +VLTHAQFSPPDGL Y+ F ++RS 
Sbjct: 123 LYVDRLDVYRVDDLDRQVVGAITDAFGKEIWKKSALVLTHAQFSPPDGLNYNHFVSKRSN 182

Query: 182 ALLKTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITTV 241
           ALLK +++GA   K D+QG +IPV+LVENSGRC+KNE DEK+LP GT+WIP+L   IT +
Sbjct: 183 ALLKVIQTGAQLKKQDLQGFSIPVILVENSGRCHKNESDEKILPCGTSWIPNLFNKITEI 242

Query: 242 VLNGSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRPSWE 301
             NG+K+I VDKKL+EGPNPN+RGK LIP +FA QYL V+KP+ RAI+SD+S+ES+P+WE
Sbjct: 243 SFNGNKAIHVDKKLVEGPNPNERGKKLIPLMFAFQYLLVMKPLVRAIKSDVSRESKPAWE 302

Query: 302 TRDTSFTSRK 312
            RD+   SR+
Sbjct: 303 LRDSGLASRR 312

[Close]

BLAST of MS002224 vs. TAIR 10
Match: AT1G02280.1 (translocon at the outer envelope membrane of chloroplasts 33 )

HSP 1 Score: 370.9 bits (951), Expect = 9.8e-103
Identity = 176/294 (59.86%), Postives = 232/294 (78.91%), Query Frame = 0


Query: 5   VIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGERAVS 64
           ++REW+G   F  ATQ+KL+E  G+LKQ++++++T+LV+GKGGVGKSSTVNS+IGE+ V 
Sbjct: 4   LVREWVGFQQFPAATQEKLIEFFGKLKQKDMNSMTVLVLGKGGVGKSSTVNSLIGEQVVR 63

Query: 65  VSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDILLYV 124
           VSPFQ+E  RPVMVSR+  GFT+N+IDTPG++E GY+N QALE+IK FL+N+TID+LLYV
Sbjct: 64  VSPFQAEGLRPVMVSRTMGGFTINIIDTPGLVEAGYVNHQALELIKGFLVNRTIDVLLYV 123

Query: 125 DRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRSEALL 184
           DRLD YRVD L+KQV+ AIT +FGK IW + L+VLTHAQFSPPD L Y+ F ++RS++LL
Sbjct: 124 DRLDVYRVDELDKQVVIAITQTFGKEIWCKTLLVLTHAQFSPPDELSYETFSSKRSDSLL 183

Query: 185 KTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITTVVLN 244
           KT+R+G+   K + + S I VV  ENSGRC+KN+KDEK LPNG AWIP+LV+ IT V  N
Sbjct: 184 KTIRAGSKMRKQEFEDSAIAVVYAENSGRCSKNDKDEKALPNGEAWIPNLVKAITDVATN 243

Query: 245 GSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRP 299
             K+I VDKK+++G   + +GK LIP I   QYL +VK I+ AIR+DI    +P
Sbjct: 244 QRKAIHVDKKMVDGSYSDDKGKKLIPLIIGAQYL-IVKMIQGAIRNDIKTSGKP 296

[Close]

BLAST of MS002224 vs. TAIR 10
Match: AT1G02280.2 (translocon at the outer envelope membrane of chloroplasts 33 )

HSP 1 Score: 370.9 bits (951), Expect = 9.8e-103
Identity = 176/294 (59.86%), Postives = 232/294 (78.91%), Query Frame = 0


Query: 5   VIREWIGINTFAMATQDKLLELLGRLKQENVSTLTILVMGKGGVGKSSTVNSIIGERAVS 64
           ++REW+G   F  ATQ+KL+E  G+LKQ++++++T+LV+GKGGVGKSSTVNS+IGE+ V 
Sbjct: 4   LVREWVGFQQFPAATQEKLIEFFGKLKQKDMNSMTVLVLGKGGVGKSSTVNSLIGEQVVR 63

Query: 65  VSPFQSEVPRPVMVSRSRAGFTLNVIDTPGIIEGGYINDQALEIIKRFLLNKTIDILLYV 124
           VSPFQ+E  RPVMVSR+  GFT+N+IDTPG++E GY+N QALE+IK FL+N+TID+LLYV
Sbjct: 64  VSPFQAEGLRPVMVSRTMGGFTINIIDTPGLVEAGYVNHQALELIKGFLVNRTIDVLLYV 123

Query: 125 DRLDAYRVDNLEKQVIKAITDSFGKAIWNRALVVLTHAQFSPPDGLPYDEFFNRRSEALL 184
           DRLD YRVD L+KQV+ AIT +FGK IW + L+VLTHAQFSPPD L Y+ F ++RS++LL
Sbjct: 124 DRLDVYRVDELDKQVVIAITQTFGKEIWCKTLLVLTHAQFSPPDELSYETFSSKRSDSLL 183

Query: 185 KTVRSGASFNKNDIQGSNIPVVLVENSGRCNKNEKDEKVLPNGTAWIPHLVETITTVVLN 244
           KT+R+G+   K + + S I VV  ENSGRC+KN+KDEK LPNG AWIP+LV+ IT V  N
Sbjct: 184 KTIRAGSKMRKQEFEDSAIAVVYAENSGRCSKNDKDEKALPNGEAWIPNLVKAITDVATN 243

Query: 245 GSKSIFVDKKLIEGPNPNQRGKLLIPFIFALQYLFVVKPIERAIRSDISKESRP 299
             K+I VDKK+++G   + +GK LIP I   QYL +VK I+ AIR+DI    +P
Sbjct: 244 QRKAIHVDKKMVDGSYSDDKGKKLIPLIIGAQYL-IVKMIQGAIRNDIKTSGKP 296

The following BLAST results are available for this feature:

BLAST of MS002224 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135187.1	9.4e-172	100.00	uncharacterized protein LOC111007215 [Momordica charantia] >XP_022135188.1 uncha...	[more]
XP_038876609.1	3.3e-161	94.55	translocase of chloroplast 34 [Benincasa hispida]	[more]
XP_016900644.1	8.8e-154	89.10	PREDICTED: translocase of chloroplast 34-like [Cucumis melo] >XP_016900645.1 PRE...	[more]
TYK11416.1	8.8e-154	89.10	translocase of chloroplast 34-like [Cucumis melo var. makuwa]	[more]
KAA0052960.1	2.0e-153	88.78	translocase of chloroplast 34-like [Cucumis melo var. makuwa]	[more]

BLAST of MS002224 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q41009	3.5e-137	80.40	Translocase of chloroplast 34 OS=Pisum sativum OX=3888 GN=TOC34 PE=1 SV=1	[more]
Q38906	1.7e-123	67.10	Translocase of chloroplast 34, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
O23680	1.4e-101	59.86	Translocase of chloroplast 33, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
A8HYJ1	7.0e-37	36.14	Translocase of chloroplast 34 homolog, chloroplastic OS=Chlamydomonas reinhardti...	[more]
Q9LUS2	8.0e-33	36.05	Translocase of chloroplast 120, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]

BLAST of MS002224 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C1Z3	4.5e-172	100.00	Translocase of chloroplast OS=Momordica charantia OX=3673 GN=LOC111007215 PE=3 S...	[more]
A0A1S4DXD3	4.3e-154	89.10	Translocase of chloroplast OS=Cucumis melo OX=3656 GN=LOC107990978 PE=3 SV=1	[more]
A0A5D3CLX1	4.3e-154	89.10	Translocase of chloroplast OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
E5RDD4	4.3e-154	89.10	Translocase of chloroplast OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]
A0A5A7UCN3	9.5e-154	88.78	Translocase of chloroplast OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]

BLAST of MS002224 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G05000.2	1.2e-124	67.10	translocon at the outer envelope membrane of chloroplasts 34	[more]
AT5G05000.3	1.2e-124	67.10	translocon at the outer envelope membrane of chloroplasts 34	[more]
AT5G05000.1	1.2e-124	67.10	translocon at the outer envelope membrane of chloroplasts 34	[more]
AT1G02280.1	9.8e-103	59.86	translocon at the outer envelope membrane of chloroplasts 33	[more]
AT1G02280.2	9.8e-103	59.86	translocon at the outer envelope membrane of chloroplasts 33	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 1..267 e-value: 5.7E-81 score: 273.8
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 7..257
IPR005688	Chloroplast protein import component Toc34	TIGRFAM	TIGR00991	TIGR00991	coord: 1..311 e-value: 8.5E-176 score: 581.5
IPR005688	Chloroplast protein import component Toc34	PIRSF	PIRSF038134	Toc33/toc34	coord: 1..309 e-value: 1.4E-165 score: 547.4
IPR006703	AIG1-type guanine nucleotide-binding (G) domain	PFAM	PF04548	AIG1	coord: 38..188 e-value: 5.2E-37 score: 127.2
IPR006703	AIG1-type guanine nucleotide-binding (G) domain	PROSITE	PS51720	G_AIG1	coord: 35..259 score: 39.282944
None	No IPR available	PIRSR	PIRSR038134-1	PIRSR038134-1	coord: 6..292 e-value: 3.2E-152 score: 503.3
None	No IPR available	PIRSR	PIRSR006230-1	PIRSR006230-1	coord: 31..96 e-value: 1.2E-5 score: 22.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 293..312
None	No IPR available	PANTHER	PTHR10903:SF125	TRANSLOCASE OF CHLOROPLAST	coord: 1..300
None	No IPR available	CDD	cd01853	Toc34_like	coord: 7..252 e-value: 1.74829E-134 score: 379.74
IPR045058	GTPase GIMA/IAN/Toc	PANTHER	PTHR10903	GTPASE, IMAP FAMILY MEMBER-RELATED	coord: 1..300

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS002224.1	MS002224.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006886	intracellular protein transport
cellular_component	GO:0009707	chloroplast outer membrane
molecular_function	GO:0005525	GTP binding
molecular_function	GO:0015450	protein-transporting ATPase activity