MS002200 (gene) Bitter gourd (TR) v1

BLAST of MS002200 vs. NCBI nr
Match: XP_022134998.1 (uncharacterized protein LOC111007109 [Momordica charantia])

HSP 1 Score: 1542.3 bits (3992), Expect = 0.0e+00
Identity = 779/784 (99.36%), Postives = 784/784 (100.00%), Query Frame = 0

Query: 1   MEQQKKKLCGDGDIHISTSSSSSRSKRKRQSNSNSNCNFEIDSTFSLLLAAVSSPRNPCS 60
           MEQQKKKLCGDGDIHISTSSSSSRSKRKRQSNSNSNCNFEIDSTFSLLLAAVSSPRNPCS
Sbjct: 1   MEQQKKKLCGDGDIHISTSSSSSRSKRKRQSNSNSNCNFEIDSTFSLLLAAVSSPRNPCS 60

Query: 61  VSLISKCLAELRLAVFPQSPRPLQTLPPPILSLLPLLILSRRPGIASRAAEIVGAASLLS 120
           VSLISKCLAELRLAVFPQSPRPLQTLPPPILSLLPLLILSRRPGIASRAAEIVGAASLLS
Sbjct: 61  VSLISKCLAELRLAVFPQSPRPLQTLPPPILSLLPLLILSRRPGIASRAAEIVGAASLLS 120

Query: 121 LRVNEEIASDDAVLKALMSALACSNRTVALAACNAVLDLSTTSFGRRSLVEVSAIERLMS 180
           LRVNEEIASDDAVLKAL+SALACSNRTVALAACNAVLDLSTTSFGRRSLVEVSAIERLMS
Sbjct: 121 LRVNEEIASDDAVLKALISALACSNRTVALAACNAVLDLSTTSFGRRSLVEVSAIERLMS 180

Query: 181 AFLQVSASSEMSVSVGTVYNENFATLKAGFRGDELSILILNAAIVLLNTCHFKHLEKIPR 240
           AFLQVSASSEMSVSVGTVYNENFATLKAGFRGDELSILILNAAIVLLNTCHFKHLEKIPR
Sbjct: 181 AFLQVSASSEMSVSVGTVYNENFATLKAGFRGDELSILILNAAIVLLNTCHFKHLEKIPR 240

Query: 241 YCSQMFLAFLKNQWIEVRNKMVLINSFECSLQEKFDISKISTNDLAVSIFRLSISTDQVS 300
           YCSQMFLAFLKNQWIEVRNKMVLINSF+CSLQEKFDISKISTNDLAVSIFRLSISTDQVS
Sbjct: 241 YCSQMFLAFLKNQWIEVRNKMVLINSFQCSLQEKFDISKISTNDLAVSIFRLSISTDQVS 300

Query: 301 RLLPVKEVKTLFGLSGSKFEEFMLNHWEASPLLMQKFSRTMNEEADIIRSFMGSVVSIDS 360
           RL+PVKEVKTLFGLSGSKFEEFMLNHWEASPLLMQKFSRTMNEEADIIRSFMGSVVSIDS
Sbjct: 301 RLIPVKEVKTLFGLSGSKFEEFMLNHWEASPLLMQKFSRTMNEEADIIRSFMGSVVSIDS 360

Query: 361 FLSPMLGRLVSCNPIASDELDIHNFLEEVKNELGFPLIYQQDIRVLRTDRCSKREIHFFQ 420
           FLSPMLGRLVSCNPIASDELDIHNFLEEVKNELGFPLIYQQDIRVLRTDRCSKREIHFFQ
Sbjct: 361 FLSPMLGRLVSCNPIASDELDIHNFLEEVKNELGFPLIYQQDIRVLRTDRCSKREIHFFQ 420

Query: 421 ENFEPCFAKGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPSV 480
           ENFEPCFAKGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPSV
Sbjct: 421 ENFEPCFAKGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPSV 480

Query: 481 GANMYLTPPGSQGFTCHYDDHCVFVCQLAGSKQWTVFSPLEMWLPRLYDAHESQHCSEIE 540
           GANMYLTPPGSQGFTCHYDDHCVFVCQLAGSKQWTVFSPLEMWLPRLYDAHESQHCSEIE
Sbjct: 481 GANMYLTPPGSQGFTCHYDDHCVFVCQLAGSKQWTVFSPLEMWLPRLYDAHESQHCSEIE 540

Query: 541 NPLAVRRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFVHIA 600
           NPLAVRRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFVHIA
Sbjct: 541 NPLAVRRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFVHIA 600

Query: 601 LYSWNDNPKQYDTSLGAMSIELLHFAIGLISISDHNFRKACLVAAISLPSDTDNRPDLNQ 660
           +YSWNDNPKQYDTSLGAMSIELLHFAIGLISISDHNFRKACLVAAISLPSDTDNRPDLNQ
Sbjct: 601 IYSWNDNPKQYDTSLGAMSIELLHFAIGLISISDHNFRKACLVAAISLPSDTDNRPDLNQ 660

Query: 661 KTIFNRLIDKISRESKFLEVLSSIEIAVEKDEDPFHRMRWLRLLNVDGKERTKEDKWTMP 720
           KTIFNRLIDKISRESKFLEV+SSIEIAVEKDEDPFHRMRWLRLLNVDGKERTKEDKWTMP
Sbjct: 661 KTIFNRLIDKISRESKFLEVISSIEIAVEKDEDPFHRMRWLRLLNVDGKERTKEDKWTMP 720

Query: 721 SIGIRDLFSICVNYKEKVENTFMEVKSRFCSEVRFENAVECYKKLLERYSKVRSQYMTGM 780
           SIGIRDLFSICVNYKEKVENTFMEVKSRFCSEVRFENAVECYKKLLERYSKVRSQYMTGM
Sbjct: 721 SIGIRDLFSICVNYKEKVENTFMEVKSRFCSEVRFENAVECYKKLLERYSKVRSQYMTGM 780

Query: 781 ISLH 785
           ISLH
Sbjct: 781 ISLH 784

BLAST of MS002200 vs. NCBI nr
Match: XP_023515380.1 (uncharacterized protein LOC111779552 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1209.5 bits (3128), Expect = 0.0e+00
Identity = 622/787 (79.03%), Postives = 679/787 (86.28%), Query Frame = 0

Query: 3   QQKKKLCGDGDIHISTSSSSSRSKRKRQSNSNSNCNFEIDSTFSLLLAAVSSPRNPCSVS 62
           +Q++KLC        + +S+S++KRKR+SNS    +F++++TFSLLLAAVS+PRNP + S
Sbjct: 2   EQQQKLCS------HSCTSTSKTKRKRRSNS----DFDVNTTFSLLLAAVSTPRNPYAAS 61

Query: 63  LISKCLAELRLAVFPQSPRPLQTLPPPILSLLPLLILSRRPGIASRAAEIVGAASLLSLR 122
           LI KCLAEL  A+ P SP PLQTLP  ILSLLPLLILS R GIASRAAEIVGAASLLSLR
Sbjct: 62  LIPKCLAELHFALLPHSPHPLQTLPSSILSLLPLLILSERQGIASRAAEIVGAASLLSLR 121

Query: 123 VNEEIASDDAVLKALMSALACSNRTVALAACNAVLDLSTTSFGRRSLVEVSAIERLMSAF 182
           +NE IAS D V++AL+SALACS R VALAACNAV+DL TTSFGR SLVE SAIERL+SAF
Sbjct: 122 MNEVIASADGVVRALISALACSKRRVALAACNAVMDLCTTSFGRESLVEFSAIERLISAF 181

Query: 183 LQVSASSEMSVSVGTVYNENFATLKAGFRGDELSILILNAAIVLLNTCHFKHLEKIPRYC 242
           LQVSASS MSVS+GTVY EN A LKAGF GDELS+ ILNAAIVLLNTCHFKHLEKIPRY 
Sbjct: 182 LQVSASSAMSVSIGTVYIENSAILKAGFEGDELSVSILNAAIVLLNTCHFKHLEKIPRYY 241

Query: 243 SQMFLAFLKNQWIEVRNKMVLINSFECSLQEKFDISKISTNDLAVSIFRLSISTDQVSRL 302
            + FL FLK QWIEVR+KM+ IN  ECS QE+F+IS ISTNDLAV IFRLSISTDQVSR+
Sbjct: 242 LKTFLTFLKKQWIEVRSKMMQINKIECSWQEQFEISNISTNDLAVCIFRLSISTDQVSRV 301

Query: 303 LPVKEVKTLFGLSGSKFEEFMLNHWEASPLLMQKFSRTMNEEADIIRSFMGSVVSID--- 362
            PVKEVKTL GLSGS FEEFMLNHWE+SP LMQK S T+NEEADII SF+GS+ SI+   
Sbjct: 302 FPVKEVKTLLGLSGSNFEEFMLNHWESSPCLMQKSSTTINEEADIIGSFVGSITSIETNL 361

Query: 363 SFLSPMLGRLVSCNPIASDELDIHNFLEEVKNELGFPLIYQQDIRVLRTDRCSKREIHFF 422
           SF+S MLGRLVSCNPIASDELDIHNFLEE +NELGFPLIYQQDIRVLRTD C KREIHFF
Sbjct: 362 SFISSMLGRLVSCNPIASDELDIHNFLEEARNELGFPLIYQQDIRVLRTDECLKREIHFF 421

Query: 423 QENFEPCFAKGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPS 482
           Q+ FEPC  KGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPS
Sbjct: 422 QKTFEPCCLKGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPS 481

Query: 483 VGANMYLTPPGSQGFTCHYDDHCVFVCQLAGSKQWTVFSPLEMWLPRLYDAHESQHCSEI 542
           VGANMYLTPPGSQG   HYDDHCVFVCQLAGSKQWTVFSP +M LPRLYDA+E   CSE+
Sbjct: 482 VGANMYLTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKMCLPRLYDANEFPRCSEV 541

Query: 543 ENPLAVRRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFVHI 602
           E+PLAV RQFLLREGDVLYIPRGFLHEARTEN+GPDGSSLHLTFGIEVEPPFEWEGFVH 
Sbjct: 542 ESPLAVGRQFLLREGDVLYIPRGFLHEARTENNGPDGSSLHLTFGIEVEPPFEWEGFVHT 601

Query: 603 ALYSWN--DNPKQYDTSLGAMSIELLHFAIGLISISDHNFRKACLVAAISLPSDTDNRPD 662
           A+Y WN  DNPKQYDT  G MS+ELLH AI LIS+SDHNFRKACLV A  LPSD +N  D
Sbjct: 602 AVYCWNWKDNPKQYDTLFGTMSVELLHLAIWLISVSDHNFRKACLVEASFLPSDNNNMLD 661

Query: 663 LNQKTIFNRLIDKISRESKFLEVLSSIEIAVEKDEDPFHRMRWLRLLNVDGKERTKEDKW 722
            NQKTIF+RLIDKISRES FLEVL+ I+I+VEKDEDPFHRMRWLRLLNVDGKE  KED+W
Sbjct: 662 QNQKTIFSRLIDKISRESNFLEVLAGIKISVEKDEDPFHRMRWLRLLNVDGKESVKEDQW 721

Query: 723 TMPSIGIRDLFSICVNYKEKVENTFMEVKSRFCSEVRFENAVECYKKLLERYSKVRSQYM 782
           TMPSIGIRDL S C  ++EKVE TF+EVKSRFC EVRFENA ECYKKLLER+  VRSQY 
Sbjct: 722 TMPSIGIRDLLSTCATHREKVETTFVEVKSRFCREVRFENAAECYKKLLERFRNVRSQYT 778

Query: 783 TGMISLH 785
            GMISLH
Sbjct: 782 IGMISLH 778

BLAST of MS002200 vs. NCBI nr
Match: XP_022987328.1 (uncharacterized protein LOC111484915 [Cucurbita maxima])

HSP 1 Score: 1208.4 bits (3125), Expect = 0.0e+00
Identity = 622/787 (79.03%), Postives = 680/787 (86.40%), Query Frame = 0

Query: 3   QQKKKLCGDGDIHISTSSSSSRSKRKRQSNSNSNCNFEIDSTFSLLLAAVSSPRNPCSVS 62
           +Q++KLC        + +S+S++KRKR+SNS    +F++++TFSLLLAAVS+PRNP + S
Sbjct: 2   EQQQKLCS------HSRTSTSKTKRKRRSNS----DFDVNTTFSLLLAAVSTPRNPYAAS 61

Query: 63  LISKCLAELRLAVFPQSPRPLQTLPPPILSLLPLLILSRRPGIASRAAEIVGAASLLSLR 122
           LI KCLAEL  A+ P SP PLQTLP  ILSLLPLLILS R GIASRAAEIVGAASLLSLR
Sbjct: 62  LIPKCLAELHFALLPHSPHPLQTLPSSILSLLPLLILSERQGIASRAAEIVGAASLLSLR 121

Query: 123 VNEEIASDDAVLKALMSALACSNRTVALAACNAVLDLSTTSFGRRSLVEVSAIERLMSAF 182
           +NE IAS D V++AL+S LACS R VALAACNAV+DLSTTSFGR SLVE SAIERL+SAF
Sbjct: 122 MNEVIASADGVVRALISTLACSKRRVALAACNAVMDLSTTSFGRESLVEFSAIERLISAF 181

Query: 183 LQVSASSEMSVSVGTVYNENFATLKAGFRGDELSILILNAAIVLLNTCHFKHLEKIPRYC 242
           LQVSASS MSV +GTVY EN A LKAGF GDELSI IL+AAIVLLNTC+FKHLEKIPRY 
Sbjct: 182 LQVSASSAMSVFIGTVYIENSAILKAGFEGDELSISILSAAIVLLNTCYFKHLEKIPRYY 241

Query: 243 SQMFLAFLKNQWIEVRNKMVLINSFECSLQEKFDISKISTNDLAVSIFRLSISTDQVSRL 302
            + FL FLK QWIEV +KM+ IN  ECS QE+F+IS ISTNDLAV IFRLSISTDQVSR+
Sbjct: 242 LKTFLTFLKKQWIEVCSKMMQINKTECSWQEQFEISNISTNDLAVCIFRLSISTDQVSRI 301

Query: 303 LPVKEVKTLFGLSGSKFEEFMLNHWEASPLLMQKFSRTMNEEADIIRSFMGSVVSID--- 362
            PVKEVK L GLSGS FEEFMLNHWE+SP LMQK S T+NEEADII SF+GS+ SI    
Sbjct: 302 FPVKEVKALLGLSGSNFEEFMLNHWESSPCLMQKSSTTINEEADIIGSFVGSITSIKTNL 361

Query: 363 SFLSPMLGRLVSCNPIASDELDIHNFLEEVKNELGFPLIYQQDIRVLRTDRCSKREIHFF 422
           SF+S MLGRLVSCNPIASDELDIHNFLEE +NELGFPLIYQQDIRVLRTD C KREIHFF
Sbjct: 362 SFISSMLGRLVSCNPIASDELDIHNFLEEARNELGFPLIYQQDIRVLRTDECLKREIHFF 421

Query: 423 QENFEPCFAKGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPS 482
           Q+NFEPC  KGPHFLKLNDA KCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPS
Sbjct: 422 QKNFEPCCFKGPHFLKLNDARKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPS 481

Query: 483 VGANMYLTPPGSQGFTCHYDDHCVFVCQLAGSKQWTVFSPLEMWLPRLYDAHESQHCSEI 542
           VGANMYLTPPGSQG   HYDDHCVFVCQLAGSKQWTVFSP +M LPRLYDA+E   CSE+
Sbjct: 482 VGANMYLTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPTKMCLPRLYDANEFPRCSEV 541

Query: 543 ENPLAVRRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFVHI 602
           E+PLAV RQFLLREGDVLYIPRGFLHEARTEN+GPDGSSLHLTFGIEVEPPFEWEGFVH 
Sbjct: 542 ESPLAVGRQFLLREGDVLYIPRGFLHEARTENNGPDGSSLHLTFGIEVEPPFEWEGFVHT 601

Query: 603 ALY--SWNDNPKQYDTSLGAMSIELLHFAIGLISISDHNFRKACLVAAISLPSDTDNRPD 662
           A+Y  +WNDNPKQYDT  G MS+ELLH AI LIS+SDHNFRKACLV A  LPSD +NRPD
Sbjct: 602 AVYCWNWNDNPKQYDTLFGTMSVELLHLAIWLISVSDHNFRKACLVEASFLPSDNNNRPD 661

Query: 663 LNQKTIFNRLIDKISRESKFLEVLSSIEIAVEKDEDPFHRMRWLRLLNVDGKERTKEDKW 722
           LNQKTIF+RLIDKISRES FLEVL+ I+I+VEKDEDPFHRMRWLRLLNVDGKE  KED+W
Sbjct: 662 LNQKTIFSRLIDKISRESNFLEVLAGIKISVEKDEDPFHRMRWLRLLNVDGKESVKEDQW 721

Query: 723 TMPSIGIRDLFSICVNYKEKVENTFMEVKSRFCSEVRFENAVECYKKLLERYSKVRSQYM 782
            MPSIGIRDL S C  ++EKVE TF+EVKSRFC EVRFENAVECYKKLLER+  VRSQY 
Sbjct: 722 IMPSIGIRDLLSTCATHREKVETTFVEVKSRFCREVRFENAVECYKKLLERFRNVRSQYT 778

Query: 783 TGMISLH 785
           TGMISLH
Sbjct: 782 TGMISLH 778

BLAST of MS002200 vs. NCBI nr
Match: XP_022921349.1 (uncharacterized protein LOC111429646 [Cucurbita moschata])

HSP 1 Score: 1202.2 bits (3109), Expect = 0.0e+00
Identity = 621/787 (78.91%), Postives = 678/787 (86.15%), Query Frame = 0

Query: 3   QQKKKLCGDGDIHISTSSSSSRSKRKRQSNSNSNCNFEIDSTFSLLLAAVSSPRNPCSVS 62
           +Q++KLC        + +S+S++KRKR+SNS    +F++++TFSLLLAAVS+P NP + S
Sbjct: 2   EQQQKLCS------HSCTSTSKTKRKRRSNS----DFDVNTTFSLLLAAVSTPHNPYAAS 61

Query: 63  LISKCLAELRLAVFPQSPRPLQTLPPPILSLLPLLILSRRPGIASRAAEIVGAASLLSLR 122
           LI KCLAEL  A+ P SP PLQTLP  ILSLLPLLILS R GIASRAAEIVGAASLLSLR
Sbjct: 62  LIPKCLAELHFALLPHSPHPLQTLPSSILSLLPLLILSERQGIASRAAEIVGAASLLSLR 121

Query: 123 VNEEIASDDAVLKALMSALACSNRTVALAACNAVLDLSTTSFGRRSLVEVSAIERLMSAF 182
           +NE IAS D V++AL+SALACS R VALAACNAV+DLSTTSFGR SLVE SAIERL+SAF
Sbjct: 122 MNEVIASADGVVRALISALACSKRRVALAACNAVMDLSTTSFGRESLVEFSAIERLISAF 181

Query: 183 LQVSASSEMSVSVGTVYNENFATLKAGFRGDELSILILNAAIVLLNTCHFKHLEKIPRYC 242
           LQVSASS MSVS+GTVY EN A LKAGF GDELSI ILNAAIVLLNTCHFKHLEKIPRY 
Sbjct: 182 LQVSASSAMSVSIGTVYIENSAILKAGFEGDELSISILNAAIVLLNTCHFKHLEKIPRYY 241

Query: 243 SQMFLAFLKNQWIEVRNKMVLINSFECSLQEKFDISKISTNDLAVSIFRLSISTDQVSRL 302
            + FL FL+ QWIEVR+KM+ IN  ECS QE+F IS ISTNDLAV IFRLSISTD+VSR+
Sbjct: 242 LKTFLTFLQKQWIEVRSKMMQINKIECSWQEQFKISNISTNDLAVCIFRLSISTDRVSRV 301

Query: 303 LPVKEVKTLFGLSGSKFEEFMLNHWEASPLLMQKFSRTMNEEADIIRSFMGSVVSID--- 362
             VKEVKTL GLSG  FEEFMLNHWE+SP LMQK S T+NEEADII SF+GS+ SI+   
Sbjct: 302 FLVKEVKTLLGLSGLNFEEFMLNHWESSPCLMQKSSTTINEEADIIGSFVGSITSIETNL 361

Query: 363 SFLSPMLGRLVSCNPIASDELDIHNFLEEVKNELGFPLIYQQDIRVLRTDRCSKREIHFF 422
           SF+S MLGRLVSCNPIASDELDIHNFLEE +NELGFPLIYQQDIRVLRTD C KREIHFF
Sbjct: 362 SFISSMLGRLVSCNPIASDELDIHNFLEEARNELGFPLIYQQDIRVLRTDECLKREIHFF 421

Query: 423 QENFEPCFAKGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPS 482
           Q+ FEPC  KGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPS
Sbjct: 422 QKTFEPCCLKGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPS 481

Query: 483 VGANMYLTPPGSQGFTCHYDDHCVFVCQLAGSKQWTVFSPLEMWLPRLYDAHESQHCSEI 542
           VGANMYLTPPGSQG   HYDDHCVFVCQLAGSKQWTVFSP +M LPRLYDA+E   CSE+
Sbjct: 482 VGANMYLTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKMCLPRLYDANEFPRCSEV 541

Query: 543 ENPLAVRRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFVHI 602
           E+PLAV RQFLLREGDVLYIPRGFLHEARTEN+GPDGSSLHLTFGIEVEPPFEWEGFVH 
Sbjct: 542 ESPLAVGRQFLLREGDVLYIPRGFLHEARTENNGPDGSSLHLTFGIEVEPPFEWEGFVHT 601

Query: 603 ALY--SWNDNPKQYDTSLGAMSIELLHFAIGLISISDHNFRKACLVAAISLPSDTDNRPD 662
           A+Y   WNDNPKQYDT  G MS+ELLH AI LIS+SDHNFRKACLV A SLPSD +N   
Sbjct: 602 AVYCWKWNDNPKQYDTLFGTMSVELLHLAIWLISVSDHNFRKACLVEASSLPSDNNNMLA 661

Query: 663 LNQKTIFNRLIDKISRESKFLEVLSSIEIAVEKDEDPFHRMRWLRLLNVDGKERTKEDKW 722
           LNQKTIF+RLIDKISRES FLEVL+ I+I+VEKDEDPFHRMRWLRLLNVD KE  KED+W
Sbjct: 662 LNQKTIFSRLIDKISRESNFLEVLAGIKISVEKDEDPFHRMRWLRLLNVDEKESVKEDQW 721

Query: 723 TMPSIGIRDLFSICVNYKEKVENTFMEVKSRFCSEVRFENAVECYKKLLERYSKVRSQYM 782
           TMPSIGIRDL S C  ++EKVE TF+EVKSRFC EVRFENAVECYKKLLER+  VRSQY 
Sbjct: 722 TMPSIGIRDLLSTCATHREKVETTFVEVKSRFCREVRFENAVECYKKLLERFRNVRSQYT 778

Query: 783 TGMISLH 785
           TGMISLH
Sbjct: 782 TGMISLH 778

BLAST of MS002200 vs. NCBI nr
Match: KAG7023352.1 (Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1194.5 bits (3089), Expect = 0.0e+00
Identity = 617/787 (78.40%), Postives = 676/787 (85.90%), Query Frame = 0

Query: 3   QQKKKLCGDGDIHISTSSSSSRSKRKRQSNSNSNCNFEIDSTFSLLLAAVSSPRNPCSVS 62
           +Q++KLC        + +S+S++KRKR+SNS    +F++++TF LLLAAVS+ RNP + S
Sbjct: 2   EQQQKLCS------HSCTSTSKTKRKRRSNS----DFDVNTTFCLLLAAVSTRRNPYAAS 61

Query: 63  LISKCLAELRLAVFPQSPRPLQTLPPPILSLLPLLILSRRPGIASRAAEIVGAASLLSLR 122
           LI KCLAEL  A+ P SP PLQTLP  ILSLLPLLILS   GIASRAAEIVGAASLLSLR
Sbjct: 62  LIPKCLAELHFALLPHSPHPLQTLPSSILSLLPLLILSELQGIASRAAEIVGAASLLSLR 121

Query: 123 VNEEIASDDAVLKALMSALACSNRTVALAACNAVLDLSTTSFGRRSLVEVSAIERLMSAF 182
           +NE IAS D V++AL+SALACS R VALAACNAV+DLSTTSFGR SLVE S IERL+SAF
Sbjct: 122 MNEVIASADGVVRALISALACSKRRVALAACNAVMDLSTTSFGRESLVEFSTIERLISAF 181

Query: 183 LQVSASSEMSVSVGTVYNENFATLKAGFRGDELSILILNAAIVLLNTCHFKHLEKIPRYC 242
           LQVSASS MSVS+GTVY EN A LKAGF GDELSI ILNAAIVLLNTCHFKHLEKIPRY 
Sbjct: 182 LQVSASSAMSVSIGTVYIENSAILKAGFEGDELSISILNAAIVLLNTCHFKHLEKIPRYY 241

Query: 243 SQMFLAFLKNQWIEVRNKMVLINSFECSLQEKFDISKISTNDLAVSIFRLSISTDQVSRL 302
            + FL FL+ QWIEVR+KM+ IN  ECS QE+F IS ISTNDLAV IFRLSISTD+VSR+
Sbjct: 242 LKTFLTFLQKQWIEVRSKMMQINKIECSWQEQFKISNISTNDLAVCIFRLSISTDRVSRV 301

Query: 303 LPVKEVKTLFGLSGSKFEEFMLNHWEASPLLMQKFSRTMNEEADIIRSFMGSVVSID--- 362
            PVKEVK L GLSGS FEEFMLNHWE+SP LMQK S T+NEEADII SF+GS+ SI+   
Sbjct: 302 FPVKEVKMLLGLSGSNFEEFMLNHWESSPCLMQKSSTTINEEADIIGSFVGSITSIETNL 361

Query: 363 SFLSPMLGRLVSCNPIASDELDIHNFLEEVKNELGFPLIYQQDIRVLRTDRCSKREIHFF 422
           SF+S MLGRLVSCNPIASDELDIHNFLEE +NELGFPLIYQQDIRVLRTD C KREIHFF
Sbjct: 362 SFISSMLGRLVSCNPIASDELDIHNFLEEARNELGFPLIYQQDIRVLRTDECLKREIHFF 421

Query: 423 QENFEPCFAKGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPS 482
           ++NFEPC  KGPHFLKLNDA KCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPS
Sbjct: 422 RKNFEPCCFKGPHFLKLNDARKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPS 481

Query: 483 VGANMYLTPPGSQGFTCHYDDHCVFVCQLAGSKQWTVFSPLEMWLPRLYDAHESQHCSEI 542
           VGANMYLTPPGSQG   HYDDHCVFVCQLAGSKQWTVFSP +M LPRLYDA+E   CSE+
Sbjct: 482 VGANMYLTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKMCLPRLYDANEFPRCSEV 541

Query: 543 ENPLAVRRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFVHI 602
           E+PLAV RQFLLREGDVLYIPRGFLHEARTEN+GPDGSSLHLTFGIEVEPPFEWEGFVH 
Sbjct: 542 ESPLAVGRQFLLREGDVLYIPRGFLHEARTENNGPDGSSLHLTFGIEVEPPFEWEGFVHT 601

Query: 603 ALY--SWNDNPKQYDTSLGAMSIELLHFAIGLISISDHNFRKACLVAAISLPSDTDNRPD 662
           A+Y  +WNDNPKQYDT  G MS+ELLH AI LIS+SDHNFRKACLV A  LPSD +N   
Sbjct: 602 AVYCWNWNDNPKQYDTLFGTMSVELLHLAIWLISVSDHNFRKACLVEASFLPSDNNNMLA 661

Query: 663 LNQKTIFNRLIDKISRESKFLEVLSSIEIAVEKDEDPFHRMRWLRLLNVDGKERTKEDKW 722
           LNQKTIF+RLIDKISRES FLEVL+ I+I+VEKDEDPFHRMRWLRLLNVD KE  KED+W
Sbjct: 662 LNQKTIFSRLIDKISRESNFLEVLAGIKISVEKDEDPFHRMRWLRLLNVDEKESVKEDQW 721

Query: 723 TMPSIGIRDLFSICVNYKEKVENTFMEVKSRFCSEVRFENAVECYKKLLERYSKVRSQYM 782
           TMPSIGIRDL S C  ++EKVE TF+EVKSRFC EVRFENAVECYKKLLER+  VRSQY 
Sbjct: 722 TMPSIGIRDLLSTCATHREKVETTFVEVKSRFCREVRFENAVECYKKLLERFRNVRSQYT 778

Query: 783 TGMISLH 785
           TGMISLH
Sbjct: 782 TGMISLH 778

BLAST of MS002200 vs. ExPASy Swiss-Prot
Match: B4GUZ2 (Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66 OS=Drosophila persimilis OX=7234 GN=GL12933 PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 3.1e-17
Identity = 48/103 (46.60%), Postives = 67/103 (65.05%), Query Frame = 0

Query: 480 VGANMYLTPPGSQGFTCHYDDHCVFVCQLAGSKQWTVFSPLEMWLPRLYDAHESQHCSEI 539
           VGAN+YLTPP SQGF  HYDD   FV Q+ G K+W +++P +  LPR    + SQ  +E+
Sbjct: 370 VGANVYLTPPESQGFAPHYDDIEAFVLQVEGKKRWRIYAPTKE-LPRESSGNLSQ--TEL 429

Query: 540 ENPLAVRRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLT 583
            +P+      +L+ GD+LY PRG++H+A TE    D  SLH+T
Sbjct: 430 GDPI---MDIVLKPGDLLYFPRGWIHQAITEK---DSHSLHIT 463

BLAST of MS002200 vs. ExPASy Swiss-Prot
Match: Q16W06 (Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66 OS=Aedes aegypti OX=7159 GN=AAEL009382 PE=3 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 7.0e-17
Identity = 76/268 (28.36%), Postives = 112/268 (41.79%), Query Frame = 0

Query: 320 EEFMLNHWEASPLLMQKFSRTMNEEADIIRSFMGSVVSIDSFLSPMLGRLVSCNPIASDE 379
           E+FM+N+WE  PLL+Q+ +                     S+ S +L R      +  + 
Sbjct: 217 EDFMINYWEKKPLLIQRKN--------------------PSYYSNLLSRAKIDEMLRQNN 276

Query: 380 LDIHNFLEEVKNELGFPLIYQQDIRVLRTDRCSKREIHFFQENFEPCFAKGPHFLKLNDA 439
           ++    ++      G    +  D RVL  D  S     F++E        G     LN  
Sbjct: 277 IEYTKNIDVTSYREGQRETHNPDGRVLPPDMWS-----FYEE--------GCSIRMLN-- 336

Query: 440 LKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMYLTPPGSQGFTCHYD 499
                   + Y   +  M  + ++           F     GAN YLTPP SQGF  HYD
Sbjct: 337 -------PQTYLPGVYEMNVKLQE-----------FFHCMTGANFYLTPPNSQGFAPHYD 396

Query: 500 DHCVFVCQLAGSKQWTVFSPLE--MWLPRLYDAHESQHCSEIENPLAVRRQFLLREGDVL 559
           D   FV Q+ G K W ++SP E    L R+   + +Q   EI  P+    + +L  GD+L
Sbjct: 397 DIEAFVLQVEGRKHWKLYSPREPAEMLARVSSPNFTQ--EEIGTPIL---EVVLEPGDLL 423

Query: 560 YIPRGFLHEARTENDGPDGSSLHLTFGI 586
           Y PRG +H+A T    P   SLH+T  +
Sbjct: 457 YFPRGIIHQASTV---PGHHSLHVTMSV 423

BLAST of MS002200 vs. ExPASy Swiss-Prot
Match: B5DUH6 (Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66 OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=GA27866 PE=3 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 7.0e-17
Identity = 48/103 (46.60%), Postives = 66/103 (64.08%), Query Frame = 0

Query: 480 VGANMYLTPPGSQGFTCHYDDHCVFVCQLAGSKQWTVFSPLEMWLPRLYDAHESQHCSEI 539
           VGAN+YLTPP SQGF  HYDD   FV Q+ G K+W +++P +  LPR    + SQ  +E+
Sbjct: 629 VGANVYLTPPESQGFAPHYDDIEAFVLQVEGKKRWRIYAPTKE-LPRESSGNLSQ--TEL 688

Query: 540 ENPLAVRRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLT 583
            +P+      +L  GD+LY PRG++H+A TE    D  SLH+T
Sbjct: 689 GDPI---MDIVLMPGDLLYFPRGWIHQAITEK---DSHSLHIT 722

BLAST of MS002200 vs. ExPASy Swiss-Prot
Match: Q5ZMM1 (Ribosomal oxygenase 1 OS=Gallus gallus OX=9031 GN=RIOX1 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 2.0e-16
Identity = 49/104 (47.12%), Postives = 63/104 (60.58%), Query Frame = 0

Query: 481 GANMYLTPPGSQGFTCHYDDHCVFVCQLAGSKQWTVFSP--LEMWLPRLYDAHESQHCSE 540
           GAN YLTPPG+QGF  HYDD   FV QL G K W V+ P      LP+   A+ +Q  +E
Sbjct: 284 GANTYLTPPGTQGFAPHYDDIEAFVLQLEGKKHWRVYGPRTSSEALPQFSSANLTQ--AE 343

Query: 541 IENPLAVRRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLT 583
           +  PL    + +L  GD+LY PRGF+H+A   +  PD  SLH+T
Sbjct: 344 LGEPLL---EVVLEAGDLLYFPRGFIHQA---DCLPDAHSLHIT 379

BLAST of MS002200 vs. ExPASy Swiss-Prot
Match: Q54K96 (Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66 OS=Dictyostelium discoideum OX=44689 GN=jcdg PE=3 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 4.5e-16
Identity = 53/139 (38.13%), Postives = 77/139 (55.40%), Query Frame = 0

Query: 446 FKEGYTIALRGMEFRYEKIAAIANTLASLFGQPSVGANMYLTPPGSQGFTCHYDDHCVFV 505
           +KEG ++ L   +     +  + +TL + F Q  VGAN+YLTP G+QGF  HYDD  VF+
Sbjct: 176 YKEGCSVRLLNPQTFNSNVWKLCSTLQTHF-QCGVGANIYLTPAGAQGFAPHYDDVDVFI 235

Query: 506 CQLAGSKQWTVFSPLEM--WLPRLYDAHESQHCSEIENPLAVRRQFLLREGDVLYIPRGF 565
            QL G K+W ++ P +    LP+    + +Q   EI  P        L  GD+LY PRG 
Sbjct: 236 LQLEGKKEWRLYKPRDANEVLPKKSSENFTQ--EEIGEPYFT---VTLEAGDLLYFPRGV 295

Query: 566 LHEARTENDGPDGSSLHLT 583
           +H+A + +   D  SLH+T
Sbjct: 296 IHQAVSPS---DVHSLHIT 305

BLAST of MS002200 vs. ExPASy TrEMBL
Match: A0A6J1C3K1 (Bifunctional lysine-specific demethylase and histidyl-hydroxylase OS=Momordica charantia OX=3673 GN=LOC111007109 PE=3 SV=1)

HSP 1 Score: 1542.3 bits (3992), Expect = 0.0e+00
Identity = 779/784 (99.36%), Postives = 784/784 (100.00%), Query Frame = 0

Query: 1   MEQQKKKLCGDGDIHISTSSSSSRSKRKRQSNSNSNCNFEIDSTFSLLLAAVSSPRNPCS 60
           MEQQKKKLCGDGDIHISTSSSSSRSKRKRQSNSNSNCNFEIDSTFSLLLAAVSSPRNPCS
Sbjct: 1   MEQQKKKLCGDGDIHISTSSSSSRSKRKRQSNSNSNCNFEIDSTFSLLLAAVSSPRNPCS 60

Query: 61  VSLISKCLAELRLAVFPQSPRPLQTLPPPILSLLPLLILSRRPGIASRAAEIVGAASLLS 120
           VSLISKCLAELRLAVFPQSPRPLQTLPPPILSLLPLLILSRRPGIASRAAEIVGAASLLS
Sbjct: 61  VSLISKCLAELRLAVFPQSPRPLQTLPPPILSLLPLLILSRRPGIASRAAEIVGAASLLS 120

Query: 121 LRVNEEIASDDAVLKALMSALACSNRTVALAACNAVLDLSTTSFGRRSLVEVSAIERLMS 180
           LRVNEEIASDDAVLKAL+SALACSNRTVALAACNAVLDLSTTSFGRRSLVEVSAIERLMS
Sbjct: 121 LRVNEEIASDDAVLKALISALACSNRTVALAACNAVLDLSTTSFGRRSLVEVSAIERLMS 180

Query: 181 AFLQVSASSEMSVSVGTVYNENFATLKAGFRGDELSILILNAAIVLLNTCHFKHLEKIPR 240
           AFLQVSASSEMSVSVGTVYNENFATLKAGFRGDELSILILNAAIVLLNTCHFKHLEKIPR
Sbjct: 181 AFLQVSASSEMSVSVGTVYNENFATLKAGFRGDELSILILNAAIVLLNTCHFKHLEKIPR 240

Query: 241 YCSQMFLAFLKNQWIEVRNKMVLINSFECSLQEKFDISKISTNDLAVSIFRLSISTDQVS 300
           YCSQMFLAFLKNQWIEVRNKMVLINSF+CSLQEKFDISKISTNDLAVSIFRLSISTDQVS
Sbjct: 241 YCSQMFLAFLKNQWIEVRNKMVLINSFQCSLQEKFDISKISTNDLAVSIFRLSISTDQVS 300

Query: 301 RLLPVKEVKTLFGLSGSKFEEFMLNHWEASPLLMQKFSRTMNEEADIIRSFMGSVVSIDS 360
           RL+PVKEVKTLFGLSGSKFEEFMLNHWEASPLLMQKFSRTMNEEADIIRSFMGSVVSIDS
Sbjct: 301 RLIPVKEVKTLFGLSGSKFEEFMLNHWEASPLLMQKFSRTMNEEADIIRSFMGSVVSIDS 360

Query: 361 FLSPMLGRLVSCNPIASDELDIHNFLEEVKNELGFPLIYQQDIRVLRTDRCSKREIHFFQ 420
           FLSPMLGRLVSCNPIASDELDIHNFLEEVKNELGFPLIYQQDIRVLRTDRCSKREIHFFQ
Sbjct: 361 FLSPMLGRLVSCNPIASDELDIHNFLEEVKNELGFPLIYQQDIRVLRTDRCSKREIHFFQ 420

Query: 421 ENFEPCFAKGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPSV 480
           ENFEPCFAKGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPSV
Sbjct: 421 ENFEPCFAKGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPSV 480

Query: 481 GANMYLTPPGSQGFTCHYDDHCVFVCQLAGSKQWTVFSPLEMWLPRLYDAHESQHCSEIE 540
           GANMYLTPPGSQGFTCHYDDHCVFVCQLAGSKQWTVFSPLEMWLPRLYDAHESQHCSEIE
Sbjct: 481 GANMYLTPPGSQGFTCHYDDHCVFVCQLAGSKQWTVFSPLEMWLPRLYDAHESQHCSEIE 540

Query: 541 NPLAVRRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFVHIA 600
           NPLAVRRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFVHIA
Sbjct: 541 NPLAVRRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFVHIA 600

Query: 601 LYSWNDNPKQYDTSLGAMSIELLHFAIGLISISDHNFRKACLVAAISLPSDTDNRPDLNQ 660
           +YSWNDNPKQYDTSLGAMSIELLHFAIGLISISDHNFRKACLVAAISLPSDTDNRPDLNQ
Sbjct: 601 IYSWNDNPKQYDTSLGAMSIELLHFAIGLISISDHNFRKACLVAAISLPSDTDNRPDLNQ 660

Query: 661 KTIFNRLIDKISRESKFLEVLSSIEIAVEKDEDPFHRMRWLRLLNVDGKERTKEDKWTMP 720
           KTIFNRLIDKISRESKFLEV+SSIEIAVEKDEDPFHRMRWLRLLNVDGKERTKEDKWTMP
Sbjct: 661 KTIFNRLIDKISRESKFLEVISSIEIAVEKDEDPFHRMRWLRLLNVDGKERTKEDKWTMP 720

Query: 721 SIGIRDLFSICVNYKEKVENTFMEVKSRFCSEVRFENAVECYKKLLERYSKVRSQYMTGM 780
           SIGIRDLFSICVNYKEKVENTFMEVKSRFCSEVRFENAVECYKKLLERYSKVRSQYMTGM
Sbjct: 721 SIGIRDLFSICVNYKEKVENTFMEVKSRFCSEVRFENAVECYKKLLERYSKVRSQYMTGM 780

Query: 781 ISLH 785
           ISLH
Sbjct: 781 ISLH 784

BLAST of MS002200 vs. ExPASy TrEMBL
Match: A0A6J1JJ52 (Bifunctional lysine-specific demethylase and histidyl-hydroxylase OS=Cucurbita maxima OX=3661 GN=LOC111484915 PE=3 SV=1)

HSP 1 Score: 1208.4 bits (3125), Expect = 0.0e+00
Identity = 622/787 (79.03%), Postives = 680/787 (86.40%), Query Frame = 0

Query: 3   QQKKKLCGDGDIHISTSSSSSRSKRKRQSNSNSNCNFEIDSTFSLLLAAVSSPRNPCSVS 62
           +Q++KLC        + +S+S++KRKR+SNS    +F++++TFSLLLAAVS+PRNP + S
Sbjct: 2   EQQQKLCS------HSRTSTSKTKRKRRSNS----DFDVNTTFSLLLAAVSTPRNPYAAS 61

Query: 63  LISKCLAELRLAVFPQSPRPLQTLPPPILSLLPLLILSRRPGIASRAAEIVGAASLLSLR 122
           LI KCLAEL  A+ P SP PLQTLP  ILSLLPLLILS R GIASRAAEIVGAASLLSLR
Sbjct: 62  LIPKCLAELHFALLPHSPHPLQTLPSSILSLLPLLILSERQGIASRAAEIVGAASLLSLR 121

Query: 123 VNEEIASDDAVLKALMSALACSNRTVALAACNAVLDLSTTSFGRRSLVEVSAIERLMSAF 182
           +NE IAS D V++AL+S LACS R VALAACNAV+DLSTTSFGR SLVE SAIERL+SAF
Sbjct: 122 MNEVIASADGVVRALISTLACSKRRVALAACNAVMDLSTTSFGRESLVEFSAIERLISAF 181

Query: 183 LQVSASSEMSVSVGTVYNENFATLKAGFRGDELSILILNAAIVLLNTCHFKHLEKIPRYC 242
           LQVSASS MSV +GTVY EN A LKAGF GDELSI IL+AAIVLLNTC+FKHLEKIPRY 
Sbjct: 182 LQVSASSAMSVFIGTVYIENSAILKAGFEGDELSISILSAAIVLLNTCYFKHLEKIPRYY 241

Query: 243 SQMFLAFLKNQWIEVRNKMVLINSFECSLQEKFDISKISTNDLAVSIFRLSISTDQVSRL 302
            + FL FLK QWIEV +KM+ IN  ECS QE+F+IS ISTNDLAV IFRLSISTDQVSR+
Sbjct: 242 LKTFLTFLKKQWIEVCSKMMQINKTECSWQEQFEISNISTNDLAVCIFRLSISTDQVSRI 301

Query: 303 LPVKEVKTLFGLSGSKFEEFMLNHWEASPLLMQKFSRTMNEEADIIRSFMGSVVSID--- 362
            PVKEVK L GLSGS FEEFMLNHWE+SP LMQK S T+NEEADII SF+GS+ SI    
Sbjct: 302 FPVKEVKALLGLSGSNFEEFMLNHWESSPCLMQKSSTTINEEADIIGSFVGSITSIKTNL 361

Query: 363 SFLSPMLGRLVSCNPIASDELDIHNFLEEVKNELGFPLIYQQDIRVLRTDRCSKREIHFF 422
           SF+S MLGRLVSCNPIASDELDIHNFLEE +NELGFPLIYQQDIRVLRTD C KREIHFF
Sbjct: 362 SFISSMLGRLVSCNPIASDELDIHNFLEEARNELGFPLIYQQDIRVLRTDECLKREIHFF 421

Query: 423 QENFEPCFAKGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPS 482
           Q+NFEPC  KGPHFLKLNDA KCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPS
Sbjct: 422 QKNFEPCCFKGPHFLKLNDARKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPS 481

Query: 483 VGANMYLTPPGSQGFTCHYDDHCVFVCQLAGSKQWTVFSPLEMWLPRLYDAHESQHCSEI 542
           VGANMYLTPPGSQG   HYDDHCVFVCQLAGSKQWTVFSP +M LPRLYDA+E   CSE+
Sbjct: 482 VGANMYLTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPTKMCLPRLYDANEFPRCSEV 541

Query: 543 ENPLAVRRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFVHI 602
           E+PLAV RQFLLREGDVLYIPRGFLHEARTEN+GPDGSSLHLTFGIEVEPPFEWEGFVH 
Sbjct: 542 ESPLAVGRQFLLREGDVLYIPRGFLHEARTENNGPDGSSLHLTFGIEVEPPFEWEGFVHT 601

Query: 603 ALY--SWNDNPKQYDTSLGAMSIELLHFAIGLISISDHNFRKACLVAAISLPSDTDNRPD 662
           A+Y  +WNDNPKQYDT  G MS+ELLH AI LIS+SDHNFRKACLV A  LPSD +NRPD
Sbjct: 602 AVYCWNWNDNPKQYDTLFGTMSVELLHLAIWLISVSDHNFRKACLVEASFLPSDNNNRPD 661

Query: 663 LNQKTIFNRLIDKISRESKFLEVLSSIEIAVEKDEDPFHRMRWLRLLNVDGKERTKEDKW 722
           LNQKTIF+RLIDKISRES FLEVL+ I+I+VEKDEDPFHRMRWLRLLNVDGKE  KED+W
Sbjct: 662 LNQKTIFSRLIDKISRESNFLEVLAGIKISVEKDEDPFHRMRWLRLLNVDGKESVKEDQW 721

Query: 723 TMPSIGIRDLFSICVNYKEKVENTFMEVKSRFCSEVRFENAVECYKKLLERYSKVRSQYM 782
            MPSIGIRDL S C  ++EKVE TF+EVKSRFC EVRFENAVECYKKLLER+  VRSQY 
Sbjct: 722 IMPSIGIRDLLSTCATHREKVETTFVEVKSRFCREVRFENAVECYKKLLERFRNVRSQYT 778

Query: 783 TGMISLH 785
           TGMISLH
Sbjct: 782 TGMISLH 778

BLAST of MS002200 vs. ExPASy TrEMBL
Match: A0A6J1E073 (Bifunctional lysine-specific demethylase and histidyl-hydroxylase OS=Cucurbita moschata OX=3662 GN=LOC111429646 PE=3 SV=1)

HSP 1 Score: 1202.2 bits (3109), Expect = 0.0e+00
Identity = 621/787 (78.91%), Postives = 678/787 (86.15%), Query Frame = 0

Query: 3   QQKKKLCGDGDIHISTSSSSSRSKRKRQSNSNSNCNFEIDSTFSLLLAAVSSPRNPCSVS 62
           +Q++KLC        + +S+S++KRKR+SNS    +F++++TFSLLLAAVS+P NP + S
Sbjct: 2   EQQQKLCS------HSCTSTSKTKRKRRSNS----DFDVNTTFSLLLAAVSTPHNPYAAS 61

Query: 63  LISKCLAELRLAVFPQSPRPLQTLPPPILSLLPLLILSRRPGIASRAAEIVGAASLLSLR 122
           LI KCLAEL  A+ P SP PLQTLP  ILSLLPLLILS R GIASRAAEIVGAASLLSLR
Sbjct: 62  LIPKCLAELHFALLPHSPHPLQTLPSSILSLLPLLILSERQGIASRAAEIVGAASLLSLR 121

Query: 123 VNEEIASDDAVLKALMSALACSNRTVALAACNAVLDLSTTSFGRRSLVEVSAIERLMSAF 182
           +NE IAS D V++AL+SALACS R VALAACNAV+DLSTTSFGR SLVE SAIERL+SAF
Sbjct: 122 MNEVIASADGVVRALISALACSKRRVALAACNAVMDLSTTSFGRESLVEFSAIERLISAF 181

Query: 183 LQVSASSEMSVSVGTVYNENFATLKAGFRGDELSILILNAAIVLLNTCHFKHLEKIPRYC 242
           LQVSASS MSVS+GTVY EN A LKAGF GDELSI ILNAAIVLLNTCHFKHLEKIPRY 
Sbjct: 182 LQVSASSAMSVSIGTVYIENSAILKAGFEGDELSISILNAAIVLLNTCHFKHLEKIPRYY 241

Query: 243 SQMFLAFLKNQWIEVRNKMVLINSFECSLQEKFDISKISTNDLAVSIFRLSISTDQVSRL 302
            + FL FL+ QWIEVR+KM+ IN  ECS QE+F IS ISTNDLAV IFRLSISTD+VSR+
Sbjct: 242 LKTFLTFLQKQWIEVRSKMMQINKIECSWQEQFKISNISTNDLAVCIFRLSISTDRVSRV 301

Query: 303 LPVKEVKTLFGLSGSKFEEFMLNHWEASPLLMQKFSRTMNEEADIIRSFMGSVVSID--- 362
             VKEVKTL GLSG  FEEFMLNHWE+SP LMQK S T+NEEADII SF+GS+ SI+   
Sbjct: 302 FLVKEVKTLLGLSGLNFEEFMLNHWESSPCLMQKSSTTINEEADIIGSFVGSITSIETNL 361

Query: 363 SFLSPMLGRLVSCNPIASDELDIHNFLEEVKNELGFPLIYQQDIRVLRTDRCSKREIHFF 422
           SF+S MLGRLVSCNPIASDELDIHNFLEE +NELGFPLIYQQDIRVLRTD C KREIHFF
Sbjct: 362 SFISSMLGRLVSCNPIASDELDIHNFLEEARNELGFPLIYQQDIRVLRTDECLKREIHFF 421

Query: 423 QENFEPCFAKGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPS 482
           Q+ FEPC  KGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPS
Sbjct: 422 QKTFEPCCLKGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPS 481

Query: 483 VGANMYLTPPGSQGFTCHYDDHCVFVCQLAGSKQWTVFSPLEMWLPRLYDAHESQHCSEI 542
           VGANMYLTPPGSQG   HYDDHCVFVCQLAGSKQWTVFSP +M LPRLYDA+E   CSE+
Sbjct: 482 VGANMYLTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPKMCLPRLYDANEFPRCSEV 541

Query: 543 ENPLAVRRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFVHI 602
           E+PLAV RQFLLREGDVLYIPRGFLHEARTEN+GPDGSSLHLTFGIEVEPPFEWEGFVH 
Sbjct: 542 ESPLAVGRQFLLREGDVLYIPRGFLHEARTENNGPDGSSLHLTFGIEVEPPFEWEGFVHT 601

Query: 603 ALY--SWNDNPKQYDTSLGAMSIELLHFAIGLISISDHNFRKACLVAAISLPSDTDNRPD 662
           A+Y   WNDNPKQYDT  G MS+ELLH AI LIS+SDHNFRKACLV A SLPSD +N   
Sbjct: 602 AVYCWKWNDNPKQYDTLFGTMSVELLHLAIWLISVSDHNFRKACLVEASSLPSDNNNMLA 661

Query: 663 LNQKTIFNRLIDKISRESKFLEVLSSIEIAVEKDEDPFHRMRWLRLLNVDGKERTKEDKW 722
           LNQKTIF+RLIDKISRES FLEVL+ I+I+VEKDEDPFHRMRWLRLLNVD KE  KED+W
Sbjct: 662 LNQKTIFSRLIDKISRESNFLEVLAGIKISVEKDEDPFHRMRWLRLLNVDEKESVKEDQW 721

Query: 723 TMPSIGIRDLFSICVNYKEKVENTFMEVKSRFCSEVRFENAVECYKKLLERYSKVRSQYM 782
           TMPSIGIRDL S C  ++EKVE TF+EVKSRFC EVRFENAVECYKKLLER+  VRSQY 
Sbjct: 722 TMPSIGIRDLLSTCATHREKVETTFVEVKSRFCREVRFENAVECYKKLLERFRNVRSQYT 778

Query: 783 TGMISLH 785
           TGMISLH
Sbjct: 782 TGMISLH 778

BLAST of MS002200 vs. ExPASy TrEMBL
Match: A0A0A0LWZ8 (Bifunctional lysine-specific demethylase and histidyl-hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_1G427540 PE=3 SV=1)

HSP 1 Score: 1147.9 bits (2968), Expect = 0.0e+00
Identity = 594/787 (75.48%), Postives = 670/787 (85.13%), Query Frame = 0

Query: 3   QQKKKLCGDGDIHISTSSSSSRSKRKRQSNSNSNCNFEIDSTFSLLLAAVSSPRNPCSVS 62
           ++KK+ C     H    +SSS++KRKRQ     + +F++++TFSLLLAAVS+P NP SVS
Sbjct: 6   KKKKEPCSYSHSH----TSSSKTKRKRQ----PHFDFDVNTTFSLLLAAVSTPHNPYSVS 65

Query: 63  LISKCLAELRLAVFPQSPRPLQTLPPPILSLLPLLILSRRPGIASRAAEIVGAASLLSLR 122
           L+ KCL  L  A+ PQSP P QTLP  ILSLLPLLILS R GIAS AAEIVGAASLLSLR
Sbjct: 66  LLPKCLTHLHSALLPQSPHPFQTLPSSILSLLPLLILSERQGIASCAAEIVGAASLLSLR 125

Query: 123 VNEEIASDDAVLKALMSALACSNRTVALAACNAVLDLSTTSFGRRSLVEVSAIERLMSAF 182
           +NE IASDD +++AL+S L CS R VALAACNAVLDLSTTSFGR+ LVE SAI+RL+   
Sbjct: 126 MNEVIASDDGLVRALISVLGCSKRRVALAACNAVLDLSTTSFGRQRLVEFSAIQRLI--V 185

Query: 183 LQVSASSEMSVSVGTVYNENFATLKAGFRGDELSILILNAAIVLLNTCHFKHLEKIPRYC 242
           + VSAS+ MSVS+GTVY EN A LKAGF GDEL I ILNAAIVL+N CHF+HLEKIP Y 
Sbjct: 186 ILVSASTGMSVSIGTVYIENSAILKAGFEGDELLISILNAAIVLINICHFEHLEKIPIYH 245

Query: 243 SQMFLAFLKNQWIEVRNKMVLINSFECSLQEKFDISKISTNDLAVSIFRLSISTDQVSRL 302
            + F+ FLKNQWIEVR+KM+ IN  ECS QE+FD+S ISTNDLA  IFRLS+STDQVSR+
Sbjct: 246 LKAFMGFLKNQWIEVRSKMMQINEIECS-QEQFDMSNISTNDLAACIFRLSMSTDQVSRV 305

Query: 303 LPVKEVKTLFGLSGSKFEEFMLNHWEASPLLMQKFSRTMNEEADIIRSFMGSVVSID--- 362
            PVKEVKTL GLSGS FEEFM++HWE SP LMQK SR +NEEADII SF+GS+ SI+   
Sbjct: 306 FPVKEVKTLLGLSGSNFEEFMVSHWETSPCLMQKSSR-INEEADIIGSFVGSITSIEKNH 365

Query: 363 SFLSPMLGRLVSCNPIASDELDIHNFLEEVKNELGFPLIYQQDIRVLRTDRCSKREIHFF 422
           SF+SPMLGRLVSC+PIASDELDIHNFLEE + ELGFPLIYQQDIRVLRTD C KREIHFF
Sbjct: 366 SFISPMLGRLVSCSPIASDELDIHNFLEEARAELGFPLIYQQDIRVLRTDECLKREIHFF 425

Query: 423 QENFEPCFAKGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQPS 482
           Q+NFEPC  +GPHFLKL+DALKCEEAFKEGYTIALRGMEFR+EKIAAI+NTLASLFGQPS
Sbjct: 426 QKNFEPCCIEGPHFLKLHDALKCEEAFKEGYTIALRGMEFRHEKIAAISNTLASLFGQPS 485

Query: 483 VGANMYLTPPGSQGFTCHYDDHCVFVCQLAGSKQWTVFSPLEMWLPRLYDAHESQHCSEI 542
           VGANMYLTPPGSQG   HYDDHCVFVCQLAGSKQWTVFSP   +LPRLYD+HE   C ++
Sbjct: 486 VGANMYLTPPGSQGLARHYDDHCVFVCQLAGSKQWTVFSPPRKYLPRLYDSHEFPSCLDV 545

Query: 543 ENPLAVRRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFVHI 602
           E+PLAV R+F LREGDVLYIPRGFLHEARTE  GPDGSSLHLTFGIEVEPPFEWEGFVH 
Sbjct: 546 ESPLAVGRKFFLREGDVLYIPRGFLHEARTEIGGPDGSSLHLTFGIEVEPPFEWEGFVHT 605

Query: 603 ALYSWN--DNPKQYDTSLGAMSIELLHFAIGLISISDHNFRKACLVAAISLPSDTDNRPD 662
           A+YSWN   NPKQ DT  G MS+ELLHFAI LIS+SDHNFRKACLVAA SLPS+T++R  
Sbjct: 606 AVYSWNWSHNPKQCDTLFGTMSLELLHFAIWLISVSDHNFRKACLVAACSLPSETNDRLG 665

Query: 663 LNQKTIFNRLIDKISRESKFLEVLSSIEIAVEKDEDPFHRMRWLRLLNVDGKERTKEDKW 722
           LN KT+F+RL+DKISRESKF EVL++I+I+VEKDEDPFHRMRWLRLLNVDGKE  K+DKW
Sbjct: 666 LNLKTVFSRLLDKISRESKFSEVLAAIKISVEKDEDPFHRMRWLRLLNVDGKESIKDDKW 725

Query: 723 TMPSIGIRDLFSICVNYKEKVENTFMEVKSRFCSEVRFENAVECYKKLLERYSKVRSQYM 782
           TMPSIGIRDLFS CVN++E+V  TF+EVKSRFCSEVRFENAVECYKKLL+R+ KVRSQY 
Sbjct: 726 TMPSIGIRDLFSTCVNHREQVATTFVEVKSRFCSEVRFENAVECYKKLLKRFRKVRSQYT 780

Query: 783 TGMISLH 785
            GMISLH
Sbjct: 786 KGMISLH 780

BLAST of MS002200 vs. ExPASy TrEMBL
Match: A0A1S3BXA4 (Bifunctional lysine-specific demethylase and histidyl-hydroxylase OS=Cucumis melo OX=3656 GN=LOC103494221 PE=3 SV=1)

HSP 1 Score: 1146.0 bits (2963), Expect = 0.0e+00
Identity = 593/788 (75.25%), Postives = 667/788 (84.64%), Query Frame = 0

Query: 2   EQQKKKLCGDGDIHISTSSSSSRSKRKRQSNSNSNCNFEIDSTFSLLLAAVSSPRNPCSV 61
           +QQKKK       HIST    S++KRKRQ     + +F++++TFSLLLAAVS+P NP SV
Sbjct: 3   QQQKKKKKLYSHSHIST----SKTKRKRQ----PHFDFDVNTTFSLLLAAVSTPHNPYSV 62

Query: 62  SLISKCLAELRLAVFPQSPRPLQTLPPPILSLLPLLILSRRPGIASRAAEIVGAASLLSL 121
            L+ KCL  L  A+ PQSP PLQTLP PILSLLPLLILS R GIAS AAEIVGAASLLSL
Sbjct: 63  FLLPKCLTHLHSALLPQSPHPLQTLPSPILSLLPLLILSERQGIASCAAEIVGAASLLSL 122

Query: 122 RVNEEIASDDAVLKALMSALACSNRTVALAACNAVLDLSTTSFGRRSLVEVSAIERLMSA 181
           R NE IA DD V++AL+S L CS R VALAACNAVLDLSTTSFGR+ LVE SAI+RL+ +
Sbjct: 123 RTNEVIALDDGVVRALISVLGCSKRRVALAACNAVLDLSTTSFGRQRLVEFSAIQRLIFS 182

Query: 182 FLQVSASSEMSVSVGTVYNENFATLKAGFRGDELSILILNAAIVLLNTCHFKHLEKIPRY 241
           FLQVSAS+ MSVS+GTVY EN A LKAG+ GDEL I ILNAAIVL+NTCHFKHLEKIP Y
Sbjct: 183 FLQVSASTGMSVSIGTVYVENSAILKAGYEGDELLISILNAAIVLINTCHFKHLEKIPSY 242

Query: 242 CSQMFLAFLKNQWIEVRNKMVLINSFECSLQEKFDISKISTNDLAVSIFRLSISTDQVSR 301
             + F+ FLKN WIEVR KM+ IN  ECS QE+FD+S ISTNDLA SIFRLS+STDQ+SR
Sbjct: 243 HLKAFMGFLKNLWIEVRIKMIQINEIECS-QEQFDMSNISTNDLAASIFRLSMSTDQISR 302

Query: 302 LLPVKEVKTLFGLSGSKFEEFMLNHWEASPLLMQKFSRTMNEEADIIRSFMGSVVSID-- 361
           + PV++VK L GLSGS FEEFM +HWEASP LMQK SR +NEEAD I SF+GS+ SI+  
Sbjct: 303 VFPVEKVKILLGLSGSNFEEFMASHWEASPCLMQKSSR-INEEADFIGSFVGSITSIEKI 362

Query: 362 -SFLSPMLGRLVSCNPIASDELDIHNFLEEVKNELGFPLIYQQDIRVLRTDRCSKREIHF 421
            SF+SPMLGRLVSC+PIASDELDIHNFLEE + ELGFPLIYQQDIRVLRTD+C KREIHF
Sbjct: 363 HSFISPMLGRLVSCSPIASDELDIHNFLEEARTELGFPLIYQQDIRVLRTDQCLKREIHF 422

Query: 422 FQENFEPCFAKGPHFLKLNDALKCEEAFKEGYTIALRGMEFRYEKIAAIANTLASLFGQP 481
           FQ+NFEPC  KGPHFLKL+DALKCEEAFKEGYTIALRGMEFR+EKIAAI+NTLASLFGQP
Sbjct: 423 FQKNFEPCCIKGPHFLKLHDALKCEEAFKEGYTIALRGMEFRHEKIAAISNTLASLFGQP 482

Query: 482 SVGANMYLTPPGSQGFTCHYDDHCVFVCQLAGSKQWTVFSPLEMWLPRLYDAHESQHCSE 541
           SVGANMYLTPPGSQG T HYDDHCVFVCQLAGSKQWTVFSP  + LPRLYD HE   CSE
Sbjct: 483 SVGANMYLTPPGSQGLTRHYDDHCVFVCQLAGSKQWTVFSPPRIRLPRLYDFHEFPSCSE 542

Query: 542 IENPLAVRRQFLLREGDVLYIPRGFLHEARTENDGPDGSSLHLTFGIEVEPPFEWEGFVH 601
           +E+ LAV RQF LREGDVLYIPRGFLHEART + GPDGSSLHLTFGIEVEPPFEWEGFVH
Sbjct: 543 VESALAVGRQFFLREGDVLYIPRGFLHEARTVSGGPDGSSLHLTFGIEVEPPFEWEGFVH 602

Query: 602 IALYSWN--DNPKQYDTSLGAMSIELLHFAIGLISISDHNFRKACLVAAISLPSDTDNRP 661
            A+YSWN   NPKQ DT  G MS++LLHFAI LIS+SDHNFRKACLVAA SLPS+T++R 
Sbjct: 603 TAVYSWNWSHNPKQCDTLFGTMSVQLLHFAIWLISVSDHNFRKACLVAACSLPSETNDRL 662

Query: 662 DLNQKTIFNRLIDKISRESKFLEVLSSIEIAVEKDEDPFHRMRWLRLLNVDGKERTKEDK 721
            L QKT F+RL+DKIS ESKF EVL++++I+VEKDEDPFHRMRWLRLLNVDGKE  K+DK
Sbjct: 663 GLIQKTTFSRLLDKISSESKFSEVLAALKISVEKDEDPFHRMRWLRLLNVDGKESIKDDK 722

Query: 722 WTMPSIGIRDLFSICVNYKEKVENTFMEVKSRFCSEVRFENAVECYKKLLERYSKVRSQY 781
           WTMPSIGIRDL S CVN++E++  TF+EVKSRFC EVRF+NAVECY+KLL+R+ KVRSQY
Sbjct: 723 WTMPSIGIRDLLSTCVNHREQIGTTFVEVKSRFCREVRFKNAVECYQKLLKRFRKVRSQY 780

Query: 782 MTGMISLH 785
             GMISLH
Sbjct: 783 TNGMISLH 780