MS002195 (gene) Bitter gourd (TR) v1

Overview


Name	MS002195
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	phospholipase A2-alpha
Location	scaffold30: 3122633 .. 3125038 (+)
RNA-Seq Expression	MS002195
Synteny	MS002195

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTCGATTTCAGGCCTTGTTGAAATTGCTTCTTCTTCTCTCTTCTTCTCTGCTCTGCCTCTCTGTGCCTGCCCGAGCCCTCAACATCGGCGTTCAGGCCATCGACGCCTCCATTTCTCTGGTTTGTTTCAAATAAATTTACCTTCCATCTTCTTCTTCTTCTTCTTCTTCTCTCTTTCTTTGTTTCTGGGTTTACTCTGTTTGCTTTCACTTGTAGAGCAAAGATTGCAGTAGGAAATGCGAGTCAGAGTTCTGTTCAGGTTCTCAATCTCTCTCTCCCTCTATGTGTTTGTGTTTGCCTTTTCAAGTTCATTGCATATTTACGGGTGAATGGAAGATTATTAACAATACTTTCTCATAACATATAACAAGTTTTTGGGTACTTAGAACAGAAGTTTGACAATTAGATTTGTTTTTAGGTTCAATTAGTAGTTTGACATCATATTTATAACAGAATTCGTCTTGTATTTGAATCTAATAAGTTTTTGGGTTCAATATGGAAAGAATCTGCAAAATCCTAGTTACCAATCAGCTAGTTGGGATCTTGTCTTCGTAGGACTTATCCCCTAAAATGTTGGAAGAAGAAATTCACGGTCGAACCCTGAAAGCAACAAGCGAGAATGGACAAGAAAAAGGAACTTGTCCACTCTCCTACTAATTCTTTTCTCTTTAATCAAAATCCATCCGTGTAGCCTTTTCCTTCCGTCGTTGTTGTCGGTACAATGCGTATAAGAAATCATCAACTATTTTCACAACCTTACTCAGGTCAGAGTAAACGAGTATTTTAGTACTGACAATTTAACAACTATCTTTGTAGCTCTAGAATGTTTCCGTTTATGCTCCTAATCCATGCAACGAAAGCGTTTAGTGATGTTATTTTCAAACTCAGAAGGGTTTAATGGTTAATCCAACACCCAATTTTTGGTCTAAAATTTGGATTTTTTTGGGCTTCAGTGCCTCCATTTTTGAGATATGGCAAATACTGTGGGCTGCTGTATAGTGGGTGCCCTGGGGAGAGGCCCTGCGATGGCTTAGATGCTTGCTGCATGAAGCACGACGACTGTGTTCAAGCCAAGAATAGTGAGTCCTCTTCCTCTTCCTTCTATATTCATTTTCTACTATAACATAGTTTGTTTTCAGTGGTTTATTTCTACCGCATTTAATAGATAAGTGGCCCCGAAAGTAATACGGGTAGGCAAGTATTGACTTTTTAGTACTCGTCTTGTTCCCCTTTGCCCAATGTGTATCACGCTTCGTTTTAATTAGTTCATTGTTAATAGAGCGTTTTGGCGTGACATGTTCTTTAGATAAATTTTGATTCGACGGGATATAATTTAGATTATATTTCTTAGTTTAATCTTGATTTAGGCTTAATCTGTCTGTTGTTGTGTCCAATAATTTTGGTGTTAGGCTGTTGATGAGAGGACTAAATTTGTCTCTTTGTTTGAGTTACCAATAAGGTCACATGTGATGTTTGGTTTACTTTATTATTGTTTATGGATTAAATGAGGGTTTAGGTTTACTTTATTGTTGCCTAATTCACCATTTGTGGAAGACAGACACAAGATAAATATGGTAAAATTTTAGGTTTTGTTTATGGGAATGAAACAACTTTTTTCCCTTCCCTTCCCTCTGTTTACCAAATTAATAGAATAGTGAAATGGGAATGTGGAGCCCATGTCTAACTTTAAATTCTCAATTTATTACCGGTTAAGGTATTACTTATCTCTCTAATGGTCAAGTATTTGAATCTCCTAGCCTCTGGTCGAACTAAAAAAAACGTATTTGGTTTGTTAAAGTTTTATTGTCGTTATTGATATGACTCTCCCTCTTGAACTTGTGAACATGTTATATCTGCAACTATCATACATACGAATAAGTGTTATGAAAAATTTTAAAAACAAAGTTGAACATAAAAAAGGAGTGTAAAAAAGGTCACACCATCTTCGAGAACTTGAATATTGTTGTGTCTAACAAGTTTCTAAGCTTTCGATATCCTAACTCATTAGATACAAAATTAAGAGTTTAAAGAGCTATTTGATACGAAATTGAAAGTTCATAAACTTGTTAGACGCGTTTTAAAGTTGAAGGATCAATTAGACATAATAATGAAAGTTTATAAACCTGTTAGAAAATTTTTATGAAACACAACGAGGTTCAAGATTCATGGACCAAACATACCATTTAACCTTAAAAGACAATGATTGGGTGTGTGGTATTTGGTTGGGCAGATGACTATCTAAGCCAAGAGTGCAGCCAAAGCTTCTTGAATTGCATGGAGAACTTCAAGAGATCAAAGCAAGCAACTTTCAAAGGAAACAAATGCCAAATTGATGAAGTCATTGATGTCATCTCACTTGTCATGGAAGCTGCCCTCATTGCTGGCAAAGTTTTTCACAAGCCA

mRNA sequence

ATGAGTCGATTTCAGGCCTTGTTGAAATTGCTTCTTCTTCTCTCTTCTTCTCTGCTCTGCCTCTCTGTGCCTGCCCGAGCCCTCAACATCGGCGTTCAGGCCATCGACGCCTCCATTTCTCTGAGCAAAGATTGCAGTAGGAAATGCGAGTCAGAGTTCTGTTCAGTGCCTCCATTTTTGAGATATGGCAAATACTGTGGGCTGCTGTATAGTGGGTGCCCTGGGGAGAGGCCCTGCGATGGCTTAGATGCTTGCTGCATGAAGCACGACGACTGTGTTCAAGCCAAGAATAATGACTATCTAAGCCAAGAGTGCAGCCAAAGCTTCTTGAATTGCATGGAGAACTTCAAGAGATCAAAGCAAGCAACTTTCAAAGGAAACAAATGCCAAATTGATGAAGTCATTGATGTCATCTCACTTGTCATGGAAGCTGCCCTCATTGCTGGCAAAGTTTTTCACAAGCCA

Coding sequence (CDS)

ATGAGTCGATTTCAGGCCTTGTTGAAATTGCTTCTTCTTCTCTCTTCTTCTCTGCTCTGCCTCTCTGTGCCTGCCCGAGCCCTCAACATCGGCGTTCAGGCCATCGACGCCTCCATTTCTCTGAGCAAAGATTGCAGTAGGAAATGCGAGTCAGAGTTCTGTTCAGTGCCTCCATTTTTGAGATATGGCAAATACTGTGGGCTGCTGTATAGTGGGTGCCCTGGGGAGAGGCCCTGCGATGGCTTAGATGCTTGCTGCATGAAGCACGACGACTGTGTTCAAGCCAAGAATAATGACTATCTAAGCCAAGAGTGCAGCCAAAGCTTCTTGAATTGCATGGAGAACTTCAAGAGATCAAAGCAAGCAACTTTCAAAGGAAACAAATGCCAAATTGATGAAGTCATTGATGTCATCTCACTTGTCATGGAAGCTGCCCTCATTGCTGGCAAAGTTTTTCACAAGCCA

Protein sequence

MSRFQALLKLLLLLSSSLLCLSVPARALNIGVQAIDASISLSKDCSRKCESEFCSVPPFLRYGKYCGLLYSGCPGERPCDGLDACCMKHDDCVQAKNNDYLSQECSQSFLNCMENFKRSKQATFKGNKCQIDEVIDVISLVMEAALIAGKVFHKP

Homology

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BLAST of MS002195 vs. NCBI nr
Match: XP_022135104.1 (phospholipase A2-alpha [Momordica charantia])

HSP 1 Score: 313.9 bits (803), Expect = 7.6e-82
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 0


Query: 1   MSRFQALLKLLLLLSSSLLCLSVPARALNIGVQAIDASISLSKDCSRKCESEFCSVPPFL 60
           MSRFQALLKLLLLLSSSLLCLSVPARALNIGVQAIDASISLSKDCSRKCESEFCSVPPFL
Sbjct: 1   MSRFQALLKLLLLLSSSLLCLSVPARALNIGVQAIDASISLSKDCSRKCESEFCSVPPFL 60

Query: 61  RYGKYCGLLYSGCPGERPCDGLDACCMKHDDCVQAKNNDYLSQECSQSFLNCMENFKRSK 120
           RYGKYCGLLYSGCPGERPCDGLDACCMKHDDCVQAKNNDYLSQECSQSFLNCMENFKRSK
Sbjct: 61  RYGKYCGLLYSGCPGERPCDGLDACCMKHDDCVQAKNNDYLSQECSQSFLNCMENFKRSK 120

Query: 121 QATFKGNKCQIDEVIDVISLVMEAALIAGKVFHKP 156
           QATFKGNKCQIDEVIDVISLVMEAALIAGKVFHKP
Sbjct: 121 QATFKGNKCQIDEVIDVISLVMEAALIAGKVFHKP 155

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BLAST of MS002195 vs. NCBI nr
Match: TYK28548.1 (phospholipase A2-alpha [Cucumis melo var. makuwa])

HSP 1 Score: 257.3 bits (656), Expect = 8.5e-65
Identity = 127/151 (84.11%), Postives = 135/151 (89.40%), Query Frame = 0


Query: 5   QALLKLLLLLSSSLLCLSVPARALNIGVQAIDASISLSKDCSRKCESEFCSVPPFLRYGK 64
           Q LL LLLLL  S+  L  P+ ALNIGVQA+DASI++SKDCSRKCESEFCSVPP LRYGK
Sbjct: 3   QFLLLLLLLLLPSIAFL--PSHALNIGVQALDASITMSKDCSRKCESEFCSVPPLLRYGK 62

Query: 65  YCGLLYSGCPGERPCDGLDACCMKHDDCVQAKNNDYLSQECSQSFLNCMENFKRSKQATF 124
           YCGLLYSGCPGERPCDGLDACCMKHD CV AKN+DYLSQECSQSFLNCMENFKRS+   F
Sbjct: 63  YCGLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSRPF 122

Query: 125 KGNKCQIDEVIDVISLVMEAALIAGKVFHKP 156
           KGNKCQ+DEVIDVIS VMEAALIAGKVFHKP
Sbjct: 123 KGNKCQVDEVIDVISFVMEAALIAGKVFHKP 151

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BLAST of MS002195 vs. NCBI nr
Match: XP_008453533.1 (PREDICTED: phospholipase A2-alpha [Cucumis melo])

HSP 1 Score: 256.9 bits (655), Expect = 1.1e-64
Identity = 127/156 (81.41%), Postives = 138/156 (88.46%), Query Frame = 0


Query: 1   MSRFQALLKLLLLLSSSLLCLS-VPARALNIGVQAIDASISLSKDCSRKCESEFCSVPPF 60
           M +F  LL LLLLL   L  ++ +P+ ALNIGVQA+DASI++SKDCSRKCESEFCSVPP 
Sbjct: 1   MPQFLLLLLLLLLLLLLLPSIAFLPSHALNIGVQALDASITMSKDCSRKCESEFCSVPPL 60

Query: 61  LRYGKYCGLLYSGCPGERPCDGLDACCMKHDDCVQAKNNDYLSQECSQSFLNCMENFKRS 120
           LRYGKYCGLLYSGCPGERPCDGLDACCMKHD CV AKN+DYLSQECSQSFLNCMENFKRS
Sbjct: 61  LRYGKYCGLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRS 120

Query: 121 KQATFKGNKCQIDEVIDVISLVMEAALIAGKVFHKP 156
           +   FKGNKCQ+DEVIDVIS VMEAALIAGKVFHKP
Sbjct: 121 RSRPFKGNKCQVDEVIDVISFVMEAALIAGKVFHKP 156

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BLAST of MS002195 vs. NCBI nr
Match: XP_038878664.1 (phospholipase A2-alpha [Benincasa hispida])

HSP 1 Score: 254.6 bits (649), Expect = 5.5e-64
Identity = 123/146 (84.25%), Postives = 133/146 (91.10%), Query Frame = 0


Query: 10  LLLLLSSSLLCLSVPARALNIGVQAIDASISLSKDCSRKCESEFCSVPPFLRYGKYCGLL 69
           LLLLL   L    +P+RALNIGVQA+DASI++SKDCSRKCESEFCSVPP LRYGKYCGLL
Sbjct: 5   LLLLL---LFIAFLPSRALNIGVQALDASITMSKDCSRKCESEFCSVPPLLRYGKYCGLL 64

Query: 70  YSGCPGERPCDGLDACCMKHDDCVQAKNNDYLSQECSQSFLNCMENFKRSKQATFKGNKC 129
           YSGCPGERPCDGLDACCMKHD CV AKN+DYLSQECSQSF+NCMENFKRSK A FKGNKC
Sbjct: 65  YSGCPGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFINCMENFKRSKSAPFKGNKC 124

Query: 130 QIDEVIDVISLVMEAALIAGKVFHKP 156
           ++DEVIDVIS VMEAALIAG+VFHKP
Sbjct: 125 EVDEVIDVISFVMEAALIAGRVFHKP 147

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BLAST of MS002195 vs. NCBI nr
Match: XP_004150744.1 (phospholipase A2-alpha [Cucumis sativus] >KGN65513.1 hypothetical protein Csa_019839 [Cucumis sativus])

HSP 1 Score: 253.1 bits (645), Expect = 1.6e-63
Identity = 121/146 (82.88%), Postives = 134/146 (91.78%), Query Frame = 0


Query: 10  LLLLLSSSLLCLSVPARALNIGVQAIDASISLSKDCSRKCESEFCSVPPFLRYGKYCGLL 69
           LLLLL  S++ L  P+ ALNIGVQA+DAS+++SKDCSRKCESEFCSVPP LRYGKYCGLL
Sbjct: 5   LLLLLLPSIVFL--PSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLL 64

Query: 70  YSGCPGERPCDGLDACCMKHDDCVQAKNNDYLSQECSQSFLNCMENFKRSKQATFKGNKC 129
           YSGCPGE PCDGLDACCMKHD CV AKN+DYLSQECSQSFLNCMENFKRS+ + FKGNKC
Sbjct: 65  YSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKC 124

Query: 130 QIDEVIDVISLVMEAALIAGKVFHKP 156
           Q+DEVI+VISLVMEAALIAG+VFHKP
Sbjct: 125 QVDEVIEVISLVMEAALIAGRVFHKP 148

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BLAST of MS002195 vs. ExPASy Swiss-Prot
Match: Q8S8N6 (Phospholipase A2-alpha OS=Arabidopsis thaliana OX=3702 GN=PLA2-ALPHA PE=1 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 6.3e-63
Identity = 114/144 (79.17%), Postives = 124/144 (86.11%), Query Frame = 0


Query: 12  LLLSSSLLCLSVPARALNIGVQAIDASISLSKDCSRKCESEFCSVPPFLRYGKYCGLLYS 71
           ++L S LL  SV   ALN+GVQ I  SISL+K+CSRKCESEFCSVPPFLRYGKYCGLLYS
Sbjct: 5   IILFSFLLFFSVSVSALNVGVQLIHPSISLTKECSRKCESEFCSVPPFLRYGKYCGLLYS 64

Query: 72  GCPGERPCDGLDACCMKHDDCVQAKNNDYLSQECSQSFLNCMENFKRSKQATFKGNKCQI 131
           GCPGERPCDGLD+CCMKHD CVQ+KNNDYLSQECSQ F+NCM NF + KQ TFKGNKC  
Sbjct: 65  GCPGERPCDGLDSCCMKHDACVQSKNNDYLSQECSQKFINCMNNFSQKKQPTFKGNKCDA 124

Query: 132 DEVIDVISLVMEAALIAGKVFHKP 156
           DEVIDVIS+VMEAALIAGKV  KP
Sbjct: 125 DEVIDVISIVMEAALIAGKVLKKP 148

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BLAST of MS002195 vs. ExPASy Swiss-Prot
Match: Q10E50 (Phospholipase A2 homolog 3 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-III PE=1 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 2.2e-44
Identity = 87/144 (60.42%), Postives = 109/144 (75.69%), Query Frame = 0


Query: 15  SSSLLCLSV---PARALNIGVQAIDASISLSKDCSRKCESEFCSVPPFLRYGKYCGLLYS 74
           +++LL  +V   PA ALNIG+Q+     S +  CSR CES+ C+ PP LRYGKYCG+LYS
Sbjct: 22  AAALLLFAVVAPPAAALNIGLQSAGDGASKAGLCSRTCESDHCTTPPLLRYGKYCGILYS 81

Query: 75  GCPGERPCDGLDACCMKHDDCVQAKNNDYLSQECSQSFLNCMENFKRSKQATFKGNKCQI 134
           GCPGE+PCD LDACCM HD+CVQAK NDYLS  C++  L C+    R   +TF+GNKC I
Sbjct: 82  GCPGEQPCDELDACCMHHDNCVQAK-NDYLSTACNEELLECLARL-REGSSTFQGNKCMI 141

Query: 135 DEVIDVISLVMEAALIAGKVFHKP 156
           DEVIDVISLV+EAA++AG++ HKP
Sbjct: 142 DEVIDVISLVIEAAVVAGRLLHKP 163

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BLAST of MS002195 vs. ExPASy Swiss-Prot
Match: Q9XG81 (Probable phospholipase A2 homolog 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-II PE=1 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 2.9e-36
Identity = 78/148 (52.70%), Postives = 100/148 (67.57%), Query Frame = 0


Query: 8   LKLLLLLSSSLLCLSVPA-RALNIGVQAIDASISLSKDCSRKCESEFCSVPPFLRYGKYC 67
           LKL    S  LL L V A R LNIG   + ++ +  + CSR CES+FC++ P LRYGKYC
Sbjct: 5   LKLAPRCSVLLLLLLVTASRGLNIG-DLLGSTPAKDQGCSRTCESQFCTIAPLLRYGKYC 64

Query: 68  GLLYSGCPGERPCDGLDACCMKHDDCVQAKNNDYLSQECSQSFLNCMENFKRSKQATFKG 127
           G+LYSGCPGERPCD LDACCM HD CV   N+DYL+  C+++ L+C++   R   ATF G
Sbjct: 65  GILYSGCPGERPCDALDACCMVHDHCVDTHNDDYLNTMCNENLLSCID---RVSGATFPG 124

Query: 128 NKCQIDEVIDVISLVMEAALIAGKVFHK 155
           NKC + +   VI  V+E A+ AGK+ HK
Sbjct: 125 NKCNVGQTASVIRGVIETAVFAGKILHK 148

[Close]

BLAST of MS002195 vs. ExPASy Swiss-Prot
Match: Q8GV50 (Phospholipase A2-delta OS=Arabidopsis thaliana OX=3702 GN=PLA2-DELTA PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 2.6e-16
Identity = 42/95 (44.21%), Postives = 58/95 (61.05%), Query Frame = 0


Query: 27  ALNIGVQAIDASISLSKDCSRKCESEFCSVPPFLRYGKYCGLLYSGCPGERPCDGLDACC 86
           AL + V  + A +   + CS+ C ++ C+V   +RYGKYCG+ Y GCPGE PCD LD CC
Sbjct: 11  ALGLTVFLLLAVVHSQEKCSKTCIAQKCNVLG-IRYGKYCGIGYFGCPGEPPCDDLDDCC 70

Query: 87  MKHDDCVQAKNNDYLSQECSQSFLNCMENFKRSKQ 122
           M HD+CV  K   Y+  +C + F  C+   K+S Q
Sbjct: 71  MTHDNCVDLKGMTYV--DCHKQFQRCVNELKQSIQ 102

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BLAST of MS002195 vs. ExPASy Swiss-Prot
Match: Q9XG80 (Probable phospholipase A2 homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-I PE=2 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 7.5e-16
Identity = 42/107 (39.25%), Postives = 61/107 (57.01%), Query Frame = 0


Query: 45  CSRKCESEFCSVPPFLRYGKYCGLLYSGCPGERPCDGLDACCMKHDDCVQAKNNDYLSQE 104
           CSR C +  C     +RYGKYCG+ +SGC GE PCD LDACC  HD CV  K    +S +
Sbjct: 29  CSRSCAALNCD-SVGIRYGKYCGVGWSGCDGEEPCDDLDACCRDHDHCVDKKG--LMSVK 88

Query: 105 CSQSFLNCMENFKRSKQATFKGNKCQIDEVIDVISLVMEAALIAGKV 152
           C + F NCM   K++ +  F   KC  +  +  ++  M+ A++  ++
Sbjct: 89  CHEKFKNCMRKVKKAGKIGF-SRKCPYEMAMATMTSGMDMAIMLSQL 131

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BLAST of MS002195 vs. ExPASy TrEMBL
Match: A0A6J1C0I6 (phospholipase A2-alpha OS=Momordica charantia OX=3673 GN=LOC111007165 PE=4 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 3.7e-82
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 0


Query: 1   MSRFQALLKLLLLLSSSLLCLSVPARALNIGVQAIDASISLSKDCSRKCESEFCSVPPFL 60
           MSRFQALLKLLLLLSSSLLCLSVPARALNIGVQAIDASISLSKDCSRKCESEFCSVPPFL
Sbjct: 1   MSRFQALLKLLLLLSSSLLCLSVPARALNIGVQAIDASISLSKDCSRKCESEFCSVPPFL 60

Query: 61  RYGKYCGLLYSGCPGERPCDGLDACCMKHDDCVQAKNNDYLSQECSQSFLNCMENFKRSK 120
           RYGKYCGLLYSGCPGERPCDGLDACCMKHDDCVQAKNNDYLSQECSQSFLNCMENFKRSK
Sbjct: 61  RYGKYCGLLYSGCPGERPCDGLDACCMKHDDCVQAKNNDYLSQECSQSFLNCMENFKRSK 120

Query: 121 QATFKGNKCQIDEVIDVISLVMEAALIAGKVFHKP 156
           QATFKGNKCQIDEVIDVISLVMEAALIAGKVFHKP
Sbjct: 121 QATFKGNKCQIDEVIDVISLVMEAALIAGKVFHKP 155

[Close]

BLAST of MS002195 vs. ExPASy TrEMBL
Match: A0A5D3DYM2 (Phospholipase A2-alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G001950 PE=4 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 4.1e-65
Identity = 127/151 (84.11%), Postives = 135/151 (89.40%), Query Frame = 0


Query: 5   QALLKLLLLLSSSLLCLSVPARALNIGVQAIDASISLSKDCSRKCESEFCSVPPFLRYGK 64
           Q LL LLLLL  S+  L  P+ ALNIGVQA+DASI++SKDCSRKCESEFCSVPP LRYGK
Sbjct: 3   QFLLLLLLLLLPSIAFL--PSHALNIGVQALDASITMSKDCSRKCESEFCSVPPLLRYGK 62

Query: 65  YCGLLYSGCPGERPCDGLDACCMKHDDCVQAKNNDYLSQECSQSFLNCMENFKRSKQATF 124
           YCGLLYSGCPGERPCDGLDACCMKHD CV AKN+DYLSQECSQSFLNCMENFKRS+   F
Sbjct: 63  YCGLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSRPF 122

Query: 125 KGNKCQIDEVIDVISLVMEAALIAGKVFHKP 156
           KGNKCQ+DEVIDVIS VMEAALIAGKVFHKP
Sbjct: 123 KGNKCQVDEVIDVISFVMEAALIAGKVFHKP 151

[Close]

BLAST of MS002195 vs. ExPASy TrEMBL
Match: A0A1S3BWG7 (phospholipase A2-alpha OS=Cucumis melo OX=3656 GN=LOC103494216 PE=4 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 5.3e-65
Identity = 127/156 (81.41%), Postives = 138/156 (88.46%), Query Frame = 0


Query: 1   MSRFQALLKLLLLLSSSLLCLS-VPARALNIGVQAIDASISLSKDCSRKCESEFCSVPPF 60
           M +F  LL LLLLL   L  ++ +P+ ALNIGVQA+DASI++SKDCSRKCESEFCSVPP 
Sbjct: 1   MPQFLLLLLLLLLLLLLLPSIAFLPSHALNIGVQALDASITMSKDCSRKCESEFCSVPPL 60

Query: 61  LRYGKYCGLLYSGCPGERPCDGLDACCMKHDDCVQAKNNDYLSQECSQSFLNCMENFKRS 120
           LRYGKYCGLLYSGCPGERPCDGLDACCMKHD CV AKN+DYLSQECSQSFLNCMENFKRS
Sbjct: 61  LRYGKYCGLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRS 120

Query: 121 KQATFKGNKCQIDEVIDVISLVMEAALIAGKVFHKP 156
           +   FKGNKCQ+DEVIDVIS VMEAALIAGKVFHKP
Sbjct: 121 RSRPFKGNKCQVDEVIDVISFVMEAALIAGKVFHKP 156

[Close]

BLAST of MS002195 vs. ExPASy TrEMBL
Match: A0A0A0LUV2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G433610 PE=4 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 7.7e-64
Identity = 121/146 (82.88%), Postives = 134/146 (91.78%), Query Frame = 0


Query: 10  LLLLLSSSLLCLSVPARALNIGVQAIDASISLSKDCSRKCESEFCSVPPFLRYGKYCGLL 69
           LLLLL  S++ L  P+ ALNIGVQA+DAS+++SKDCSRKCESEFCSVPP LRYGKYCGLL
Sbjct: 5   LLLLLLPSIVFL--PSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCGLL 64

Query: 70  YSGCPGERPCDGLDACCMKHDDCVQAKNNDYLSQECSQSFLNCMENFKRSKQATFKGNKC 129
           YSGCPGE PCDGLDACCMKHD CV AKN+DYLSQECSQSFLNCMENFKRS+ + FKGNKC
Sbjct: 65  YSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGNKC 124

Query: 130 QIDEVIDVISLVMEAALIAGKVFHKP 156
           Q+DEVI+VISLVMEAALIAG+VFHKP
Sbjct: 125 QVDEVIEVISLVMEAALIAGRVFHKP 148

[Close]

BLAST of MS002195 vs. ExPASy TrEMBL
Match: A9PB93 (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_018G132100 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.5e-62
Identity = 120/151 (79.47%), Postives = 132/151 (87.42%), Query Frame = 0


Query: 8   LKLLLLLSSSLLCL---SVPARALNIGVQAIDASISLSKDCSRKCESEFCSVPPFLRYGK 67
           LKL LL+S SLL L   S   +ALNIGVQ  D++ISLSKDCSRKCESEFCSVPPFLRYGK
Sbjct: 9   LKLALLVSCSLLVLAFSSFSVQALNIGVQTTDSAISLSKDCSRKCESEFCSVPPFLRYGK 68

Query: 68  YCGLLYSGCPGERPCDGLDACCMKHDDCVQAKNNDYLSQECSQSFLNCMENFKRSKQATF 127
           YCGLLYSGCPGE+PCDGLDACCMKHD CVQAKNNDYLSQECSQ+F+NCM NF+ S   TF
Sbjct: 69  YCGLLYSGCPGEKPCDGLDACCMKHDACVQAKNNDYLSQECSQNFINCMNNFRNSGAHTF 128

Query: 128 KGNKCQIDEVIDVISLVMEAALIAGKVFHKP 156
           KGNKCQ+DEVIDVIS+VMEAAL+AG+  HKP
Sbjct: 129 KGNKCQVDEVIDVISVVMEAALLAGRALHKP 159

[Close]

BLAST of MS002195 vs. TAIR 10
Match: AT2G06925.1 (Phospholipase A2 family protein )

HSP 1 Score: 241.5 bits (615), Expect = 4.5e-64
Identity = 114/144 (79.17%), Postives = 124/144 (86.11%), Query Frame = 0


Query: 12  LLLSSSLLCLSVPARALNIGVQAIDASISLSKDCSRKCESEFCSVPPFLRYGKYCGLLYS 71
           ++L S LL  SV   ALN+GVQ I  SISL+K+CSRKCESEFCSVPPFLRYGKYCGLLYS
Sbjct: 5   IILFSFLLFFSVSVSALNVGVQLIHPSISLTKECSRKCESEFCSVPPFLRYGKYCGLLYS 64

Query: 72  GCPGERPCDGLDACCMKHDDCVQAKNNDYLSQECSQSFLNCMENFKRSKQATFKGNKCQI 131
           GCPGERPCDGLD+CCMKHD CVQ+KNNDYLSQECSQ F+NCM NF + KQ TFKGNKC  
Sbjct: 65  GCPGERPCDGLDSCCMKHDACVQSKNNDYLSQECSQKFINCMNNFSQKKQPTFKGNKCDA 124

Query: 132 DEVIDVISLVMEAALIAGKVFHKP 156
           DEVIDVIS+VMEAALIAGKV  KP
Sbjct: 125 DEVIDVISIVMEAALIAGKVLKKP 148

[Close]

BLAST of MS002195 vs. TAIR 10
Match: AT4G29470.1 (Phospholipase A2 family protein )

HSP 1 Score: 86.7 bits (213), Expect = 1.8e-17
Identity = 42/95 (44.21%), Postives = 58/95 (61.05%), Query Frame = 0


Query: 27  ALNIGVQAIDASISLSKDCSRKCESEFCSVPPFLRYGKYCGLLYSGCPGERPCDGLDACC 86
           AL + V  + A +   + CS+ C ++ C+V   +RYGKYCG+ Y GCPGE PCD LD CC
Sbjct: 11  ALGLTVFLLLAVVHSQEKCSKTCIAQKCNVLG-IRYGKYCGIGYFGCPGEPPCDDLDDCC 70

Query: 87  MKHDDCVQAKNNDYLSQECSQSFLNCMENFKRSKQ 122
           M HD+CV  K   Y+  +C + F  C+   K+S Q
Sbjct: 71  MTHDNCVDLKGMTYV--DCHKQFQRCVNELKQSIQ 102

[Close]

BLAST of MS002195 vs. TAIR 10
Match: AT2G19690.2 (phospholipase A2-beta )

HSP 1 Score: 84.0 bits (206), Expect = 1.2e-16
Identity = 43/122 (35.25%), Postives = 69/122 (56.56%), Query Frame = 0


Query: 39  ISLSKDCSRKCESEFCSVPPFLRYGKYCGLLYSGCPGERPCDGLDACCMKHDDCVQAKNN 98
           ++ S++C+R C ++ C     +RYGKYCG+ +SGCPGE PCD LDACC  HD CV+   N
Sbjct: 25  VARSEECTRTCIAQNCDTLS-IRYGKYCGIGHSGCPGEEPCDDLDACCKIHDHCVEL--N 84

Query: 99  DYLSQECSQSFLNCMENFKRS-KQATFK----GNKCQIDEVIDVISLVMEAALIAGKV-F 155
              +  C + F  C+    ++ KQ+  K      KC    VI  ++  M+  ++  ++ +
Sbjct: 85  GMTNISCHKKFQRCVNRLSKAIKQSKNKKVGFSTKCPYSVVIPTVNQGMDIGILFSQLAY 143

[Close]

BLAST of MS002195 vs. TAIR 10
Match: AT4G29460.1 (Phospholipase A2 family protein )

HSP 1 Score: 83.2 bits (204), Expect = 2.0e-16
Identity = 38/83 (45.78%), Postives = 51/83 (61.45%), Query Frame = 0


Query: 37  ASISLSKDCSRKCESEFCSVPPFLRYGKYCGLLYSGCPGERPCDGLDACCMKHDDCVQAK 96
           A +S  + CS  C ++ C+    +RYGKYCG+ Y GCPGE PCD LDACCM HD+CV  K
Sbjct: 21  AVVSSQEKCSNTCIAQNCN-SLGIRYGKYCGIGYFGCPGEPPCDDLDACCMTHDNCVDLK 80

Query: 97  NNDYLSQECSQSFLNCMENFKRS 120
              Y++  C + F  C+    +S
Sbjct: 81  GMTYVN--CHKQFKRCVNKLSKS 100

[Close]

BLAST of MS002195 vs. TAIR 10
Match: AT2G19690.1 (phospholipase A2-beta )

HSP 1 Score: 82.8 bits (203), Expect = 2.7e-16
Identity = 42/114 (36.84%), Postives = 64/114 (56.14%), Query Frame = 0


Query: 39  ISLSKDCSRKCESEFCSVPPFLRYGKYCGLLYSGCPGERPCDGLDACCMKHDDCVQAKNN 98
           ++ S++C+R C ++ C     +RYGKYCG+ +SGCPGE PCD LDACC  HD CV+   N
Sbjct: 25  VARSEECTRTCIAQNCDTLS-IRYGKYCGIGHSGCPGEEPCDDLDACCKIHDHCVEL--N 84

Query: 99  DYLSQECSQSFLNCMENFKRS-KQATFK----GNKCQIDEVIDVISLVMEAALI 148
              +  C + F  C+    ++ KQ+  K      KC    VI  ++  M+  ++
Sbjct: 85  GMTNISCHKKFQRCVNRLSKAIKQSKNKKVGFSTKCPYSVVIPTVNQGMDIGIL 135

The following BLAST results are available for this feature:

BLAST of MS002195 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135104.1	7.6e-82	100.00	phospholipase A2-alpha [Momordica charantia]	[more]
TYK28548.1	8.5e-65	84.11	phospholipase A2-alpha [Cucumis melo var. makuwa]	[more]
XP_008453533.1	1.1e-64	81.41	PREDICTED: phospholipase A2-alpha [Cucumis melo]	[more]
XP_038878664.1	5.5e-64	84.25	phospholipase A2-alpha [Benincasa hispida]	[more]
XP_004150744.1	1.6e-63	82.88	phospholipase A2-alpha [Cucumis sativus] >KGN65513.1 hypothetical protein Csa_01...	[more]

BLAST of MS002195 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8S8N6	6.3e-63	79.17	Phospholipase A2-alpha OS=Arabidopsis thaliana OX=3702 GN=PLA2-ALPHA PE=1 SV=1	[more]
Q10E50	2.2e-44	60.42	Phospholipase A2 homolog 3 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-III ...	[more]
Q9XG81	2.9e-36	52.70	Probable phospholipase A2 homolog 2 OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]
Q8GV50	2.6e-16	44.21	Phospholipase A2-delta OS=Arabidopsis thaliana OX=3702 GN=PLA2-DELTA PE=2 SV=1	[more]
Q9XG80	7.5e-16	39.25	Probable phospholipase A2 homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]

BLAST of MS002195 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C0I6	3.7e-82	100.00	phospholipase A2-alpha OS=Momordica charantia OX=3673 GN=LOC111007165 PE=4 SV=1	[more]
A0A5D3DYM2	4.1e-65	84.11	Phospholipase A2-alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold6...	[more]
A0A1S3BWG7	5.3e-65	81.41	phospholipase A2-alpha OS=Cucumis melo OX=3656 GN=LOC103494216 PE=4 SV=1	[more]
A0A0A0LUV2	7.7e-64	82.88	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G433610 PE=4 SV=1	[more]
A9PB93	2.5e-62	79.47	Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_018G132100 PE=2 ...	[more]

BLAST of MS002195 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G06925.1	4.5e-64	79.17	Phospholipase A2 family protein	[more]
AT4G29470.1	1.8e-17	44.21	Phospholipase A2 family protein	[more]
AT2G19690.2	1.2e-16	35.25	phospholipase A2-beta	[more]
AT4G29460.1	2.0e-16	45.78	Phospholipase A2 family protein	[more]
AT2G19690.1	2.7e-16	36.84	phospholipase A2-beta	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036444	Phospholipase A2 domain superfamily	GENE3D	1.20.90.10	Phospholipase A2 domain	coord: 26..155 e-value: 5.0E-48 score: 164.0
IPR036444	Phospholipase A2 domain superfamily	SUPERFAMILY	48619	Phospholipase A2, PLA2	coord: 59..154
IPR001211	Phospholipase A2	PANTHER	PTHR11716	PHOSPHOLIPASE A2 FAMILY MEMBER	coord: 17..152
None	No IPR available	PANTHER	PTHR11716:SF87	PHOSPHOLIPASE A2 HOMOLOG 2-RELATED	coord: 17..152
None	No IPR available	CDD	cd04706	PLA2_plant	coord: 39..155 e-value: 9.61992E-62 score: 184.174
IPR033113	Phospholipase A2, histidine active site	PROSITE	PS00118	PA2_HIS	coord: 85..92

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS002195.1	MS002195.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050482	arachidonic acid secretion
biological_process	GO:0016042	lipid catabolic process
biological_process	GO:0006644	phospholipid metabolic process
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0004623	phospholipase A2 activity