MS002193 (gene) Bitter gourd (TR) v1

Overview
NameMS002193
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionsodium transporter HKT1-like
Locationscaffold30: 3113287 .. 3116226 (+)
RNA-Seq ExpressionMS002193
SyntenyMS002193
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAGGTAAATCGGTGTGCTTTATTTTGGTATGCTTCTTTCGTTTTCTTCTTCTCGTGAATCCATTTTGGATTCAACTCTTCTATTTTTTATTCATCTCGGCTTTTGGATTGTGGGTTTTGGCTGTTTTGAAGCCGAAAACTTACGATTCTTATCGACCTGCGAATTTGGATCTTTTATTCACCTCTGTGTCTGCCTCCACTGTTTCCAGCATGTCTAGTGTGGAAATGGAGGTTTTCTCTGATTCCCAACTCATTGTCTTGATTGTTTTGATGTTCGTGGGCGGCGAGGTTTTTACTTCCATGGTTGGAATCCAATTAAGGAAGTTGAAACTCAAACTTTCAGAAGATAAAATCACTTCTGTCCCTAGTGATTTAAGTACTATTTCTTCAAACCCTCAAAATATTTTAAGTCAAATAGAGTTGGGCGTCGTTAGAAAACTTGATCACCAGCCCGAGAGGCAGCTAAAACACAACTCCATCAAACTTTTGGGTATCGTAGTACTCAGTTATCTTCTAACAATCCATCTTTTAGGCATAGGGATGGTCTTAGTTTACATGGAAACTGTCTCAAGTGCTAGAGAAGTCCTAAGCAAAAAGGGGTTAAAAACATCAACTTTTGCAATTTTCCTCACGGTTTCAACTTTTGCAAGCTGTGGCTTTGTGCCAACAAATGAAAACATGATAGTCTTTAGCAAGAATTTAGGCCTACTCGCAATGCTCATTCCTCAAGCTCTCTTAGGGAACACTTTGTTTCCTTGTTGCTTGAGGTTTTGCATTTGGAGTTTGGGAAAGTTTACGAAGAAGTCAGAAACCAGATTCTTGTTGAAAAATAGTGAGGAAATTGGGTACTTGCATCTGCTCCCAAGGCTTCATTCATTGCTTTTGGCTTCAACTGTGTTTGGATTTGTGTTGATACAATCTGTAGCAATTTGGTTCATGGAGTGGAGTTCAGAAGCATTGAGTGGATTGAATTCTATTGAGAAGTTGCTTGGAGTGCTATTTTTAAGCGTAAATTCAAGGCATAGTGGTGAATCTGTTGTTGATCTCTCCATGCTCTCTCCTGCCATCTTGTTTCTCTTTGTTGCCATGATGTTAGTTCCTCTCTCTCTCTCCATTAAATGCATTCTATTTGTTTAGTATATATAGAAATATTAACTTGGTTTTTTAGTACGATATATGGGGATAAGATTCGAACTCGCGATCTTGTGGTCGGAGATAGGTGCATATATAAACCAGTTGAACACTATAGGACTGGTTTGGTAATATGTCATTTCTTGTTTCTTGTTTTCAAGTTTGTTAAAATATCTAAGCATTTTTCATCGTTTTTTGTTTCTCATTCTTTAGAAATGTTCTCAAAATCGTATAGGAATTTTGAAAACAACACAAATAAATTTCCTATTTTTTGTTCTAGTTTTCAATTAACAAAGGGTAAATAAAACTATTGTTCTATTAAAACAAAAAGGAAAAAAAATTGAGAAGGTTACTAAACTGGTTGTTAGTATAGTCTCTCCAAAAGGTCAAATTTATACTATTTACTAGAAATTTCAATGATTCTTTTATCGAAAATTTTACATTTTCTTTATGGAGAAAAAAAAAAGGTAGTAATACACTCGTCAATTTCTTGTGCTACTTAGANTATATATTAGATTACTTAGAAAAAAAGAAAAAAGAAAAAAAAAAAAAGAGGGAGAGCCTATATTGACTATCAAGAGTATTATTAGAATAGTCCTCAAGAGAAAAAAAAAGAGTATTATTAGAATAAACTAATTAATAAGTTATGTACCCAAATTTCCTTGTAATATATAAAAAATAGTTAGCAATCCTTAATAATTTTAACATTTTTTTAAGGATTACCTATGTCTGATGGGTGTCTAAAGAATTCCGGTAAACGAAAAAGGAAAAAATAAATAAAAGGTCTTAATTATATTTTAATTATTCGTAATTACTTGACTTATGTCATCCCTATTATTATATATACAAATAAAATAATAATAATTATTATTATTAATTATTCATAAAAACAAACATAGCATTAATTATCTCAATGTGAACATCTTGACTAAGAGGTTATAAATTCGAATCTTCCATACCCATTTGTTAACATAGCATTAATTATTTTTATTTTTATTTTGTCGTTGATATGGGTTAATTTAACAGGTATCTTCCACCTTATACATCTTTCATGCCAATAAAGGGTGATGGAGAAGAAGGAGAAAATCAATTGGAAGCCAATAAAATAATTAAAAAAAATAAAAGGGCCAAATTTGTGGATAATTTCATCTTCTCCCAAATTTCCTATCTGCTTATTTTCATCTTTGTTATATGCATTACAGAGAGACATAACATCAAGGAAGATCCTCTCAATTTCAATCTCTTCAACATTATTTTTGAAGTTATCAGGTAATTTTTATTCTTTACTAATTAATTTATAGTGAAAATAAATCTTAAAAAAATAATTTTCCTTTCCAATATATGACTTTTCATTTTTTTTTCCTTGTCTACTTAATGGTCATAATTCAAAAACCATAGGTGAAGAATTGTAATATTAATTTTAAAACTAAAACGTTAAAGTATACGTCATAAATAAGATCTAAATATTGTTATATTCTCATAAAATTAAGATTATCATTTTGTACAAATGGTGGATTTTTTAATTCTCAAATTATGTATTGGAAAAAAAAAAAAAGACTGTTCATAATTTTTCTTCGTCTAAATTAAATTTATGAAATATGTCTCTATTTCGACAGTGCATATGGAAATGTGGGGTTTACGATGGGATATAGCTGCAAACGACAACTTCATCCTCAAAGCAATTGTGTCGACAAGTCGTTTGGATTTTCAGGAAAGTGGAGCAGCAAAGGAAAGATAGTTCTTATTGTTGTTATGATCTTTGGACGACTCAAAAAATTCAATATGAACAGAGAGAGAGCTTGGAAACTT

mRNA sequence

TTAGGTAAATCGGTGTGCTTTATTTTGGTATGCTTCTTTCGTTTTCTTCTTCTCGTGAATCCATTTTGGATTCAACTCTTCTATTTTTTATTCATCTCGGCTTTTGGATTGTGGGTTTTGGCTGTTTTGAAGCCGAAAACTTACGATTCTTATCGACCTGCGAATTTGGATCTTTTATTCACCTCTGTGTCTGCCTCCACTGTTTCCAGCATGTCTAGTGTGGAAATGGAGGTTTTCTCTGATTCCCAACTCATTGTCTTGATTGTTTTGATGTTCGTGGGCGGCGAGGTTTTTACTTCCATGGTTGGAATCCAATTAAGGAAGTTGAAACTCAAACTTTCAGAAGATAAAATCACTTCTGTCCCTAGTGATTTAAGTACTATTTCTTCAAACCCTCAAAATATTTTAAGTCAAATAGAGTTGGGCGTCGTTAGAAAACTTGATCACCAGCCCGAGAGGCAGCTAAAACACAACTCCATCAAACTTTTGGGTATCGTAGTACTCAGTTATCTTCTAACAATCCATCTTTTAGGCATAGGGATGGTCTTAGTTTACATGGAAACTGTCTCAAGTGCTAGAGAAGTCCTAAGCAAAAAGGGGTTAAAAACATCAACTTTTGCAATTTTCCTCACGGTTTCAACTTTTGCAAGCTGTGGCTTTGTGCCAACAAATGAAAACATGATAGTCTTTAGCAAGAATTTAGGCCTACTCGCAATGCTCATTCCTCAAGCTCTCTTAGGGAACACTTTGTTTCCTTGTTGCTTGAGGTTTTGCATTTGGAGTTTGGGAAAGTTTACGAAGAAGTCAGAAACCAGATTCTTGTTGAAAAATAGTGAGGAAATTGGGTACTTGCATCTGCTCCCAAGGCTTCATTCATTGCTTTTGGCTTCAACTGTGTTTGGATTTGTGTTGATACAATCTGTAGCAATTTGGTTCATGGAGTGGAGTTCAGAAGCATTGAGTGGATTGAATTCTATTGAGAAGTTGCTTGGAGTGCTATTTTTAAGCGTAAATTCAAGGCATAGTGGTGAATCTGTTGTTGATCTCTCCATGCTCTCTCCTGCCATCTTGTTTCTCTTTGTTGCCATGATGTATCTTCCACCTTATACATCTTTCATGCCAATAAAGGGTGATGGAGAAGAAGGAGAAAATCAATTGGAAGCCAATAAAATAATTAAAAAAAATAAAAGGGCCAAATTTGTGGATAATTTCATCTTCTCCCAAATTTCCTATCTGCTTATTTTCATCTTTGTTATATGCATTACAGAGAGACATAACATCAAGGAAGATCCTCTCAATTTCAATCTCTTCAACATTATTTTTGAAGTTATCAGTGCATATGGAAATGTGGGGTTTACGATGGGATATAGCTGCAAACGACAACTTCATCCTCAAAGCAATTGTGTCGACAAGTCGTTTGGATTTTCAGGAAAGTGGAGCAGCAAAGGAAAGATAGTTCTTATTGTTGTTATGATCTTTGGACGACTCAAAAAATTCAATATGAACAGAGAGAGAGCTTGGAAACTT

Coding sequence (CDS)

TTAGGTAAATCGGTGTGCTTTATTTTGGTATGCTTCTTTCGTTTTCTTCTTCTCGTGAATCCATTTTGGATTCAACTCTTCTATTTTTTATTCATCTCGGCTTTTGGATTGTGGGTTTTGGCTGTTTTGAAGCCGAAAACTTACGATTCTTATCGACCTGCGAATTTGGATCTTTTATTCACCTCTGTGTCTGCCTCCACTGTTTCCAGCATGTCTAGTGTGGAAATGGAGGTTTTCTCTGATTCCCAACTCATTGTCTTGATTGTTTTGATGTTCGTGGGCGGCGAGGTTTTTACTTCCATGGTTGGAATCCAATTAAGGAAGTTGAAACTCAAACTTTCAGAAGATAAAATCACTTCTGTCCCTAGTGATTTAAGTACTATTTCTTCAAACCCTCAAAATATTTTAAGTCAAATAGAGTTGGGCGTCGTTAGAAAACTTGATCACCAGCCCGAGAGGCAGCTAAAACACAACTCCATCAAACTTTTGGGTATCGTAGTACTCAGTTATCTTCTAACAATCCATCTTTTAGGCATAGGGATGGTCTTAGTTTACATGGAAACTGTCTCAAGTGCTAGAGAAGTCCTAAGCAAAAAGGGGTTAAAAACATCAACTTTTGCAATTTTCCTCACGGTTTCAACTTTTGCAAGCTGTGGCTTTGTGCCAACAAATGAAAACATGATAGTCTTTAGCAAGAATTTAGGCCTACTCGCAATGCTCATTCCTCAAGCTCTCTTAGGGAACACTTTGTTTCCTTGTTGCTTGAGGTTTTGCATTTGGAGTTTGGGAAAGTTTACGAAGAAGTCAGAAACCAGATTCTTGTTGAAAAATAGTGAGGAAATTGGGTACTTGCATCTGCTCCCAAGGCTTCATTCATTGCTTTTGGCTTCAACTGTGTTTGGATTTGTGTTGATACAATCTGTAGCAATTTGGTTCATGGAGTGGAGTTCAGAAGCATTGAGTGGATTGAATTCTATTGAGAAGTTGCTTGGAGTGCTATTTTTAAGCGTAAATTCAAGGCATAGTGGTGAATCTGTTGTTGATCTCTCCATGCTCTCTCCTGCCATCTTGTTTCTCTTTGTTGCCATGATGTATCTTCCACCTTATACATCTTTCATGCCAATAAAGGGTGATGGAGAAGAAGGAGAAAATCAATTGGAAGCCAATAAAATAATTAAAAAAAATAAAAGGGCCAAATTTGTGGATAATTTCATCTTCTCCCAAATTTCCTATCTGCTTATTTTCATCTTTGTTATATGCATTACAGAGAGACATAACATCAAGGAAGATCCTCTCAATTTCAATCTCTTCAACATTATTTTTGAAGTTATCAGTGCATATGGAAATGTGGGGTTTACGATGGGATATAGCTGCAAACGACAACTTCATCCTCAAAGCAATTGTGTCGACAAGTCGTTTGGATTTTCAGGAAAGTGGAGCAGCAAAGGAAAGATAGTTCTTATTGTTGTTATGATCTTTGGACGACTCAAAAAATTCAATATGAACAGAGAGAGAGCTTGGAAACTT

Protein sequence

LGKSVCFILVCFFRFLLLVNPFWIQLFYFLFISAFGLWVLAVLKPKTYDSYRPANLDLLFTSVSASTVSSMSSVEMEVFSDSQLIVLIVLMFVGGEVFTSMVGIQLRKLKLKLSEDKITSVPSDLSTISSNPQNILSQIELGVVRKLDHQPERQLKHNSIKLLGIVVLSYLLTIHLLGIGMVLVYMETVSSAREVLSKKGLKTSTFAIFLTVSTFASCGFVPTNENMIVFSKNLGLLAMLIPQALLGNTLFPCCLRFCIWSLGKFTKKSETRFLLKNSEEIGYLHLLPRLHSLLLASTVFGFVLIQSVAIWFMEWSSEALSGLNSIEKLLGVLFLSVNSRHSGESVVDLSMLSPAILFLFVAMMYLPPYTSFMPIKGDGEEGENQLEANKIIKKNKRAKFVDNFIFSQISYLLIFIFVICITERHNIKEDPLNFNLFNIIFEVISAYGNVGFTMGYSCKRQLHPQSNCVDKSFGFSGKWSSKGKIVLIVVMIFGRLKKFNMNRERAWKL
Homology
BLAST of MS002193 vs. NCBI nr
Match: XP_022134789.1 (sodium transporter HKT1-like [Momordica charantia])

HSP 1 Score: 962.2 bits (2486), Expect = 1.7e-276
Identity = 505/509 (99.21%), Postives = 507/509 (99.61%), Query Frame = 0

Query: 1   LGKSVCFILVCFFRFLLLVNPFWIQLFYFLFISAFGLWVLAVLKPKTYDSYRPANLDLLF 60
           LGKSVCFILVCFFRFLLL+NPFWIQLFYFLFISAFGLWVLAVLKPKTYDSYRPANLDLLF
Sbjct: 28  LGKSVCFILVCFFRFLLLMNPFWIQLFYFLFISAFGLWVLAVLKPKTYDSYRPANLDLLF 87

Query: 61  TSVSASTVSSMSSVEMEVFSDSQLIVLIVLMFVGGEVFTSMVGIQLRKLKLKLSEDKITS 120
           TSVSASTVSSMSSVEMEVFSDSQLIVLIVLMFVGGEVFTSMVGIQLRKLKLKLSEDKITS
Sbjct: 88  TSVSASTVSSMSSVEMEVFSDSQLIVLIVLMFVGGEVFTSMVGIQLRKLKLKLSEDKITS 147

Query: 121 VPSDLSTISSNPQNILSQIELGVVRKLDHQPERQLKHNSIKLLGIVVLSYLLTIHLLGIG 180
           VPS LSTISSNPQNILSQIELGVVRKLDHQPERQLKHNSIKLLGIVVLSYLLTIHLLGIG
Sbjct: 148 VPSVLSTISSNPQNILSQIELGVVRKLDHQPERQLKHNSIKLLGIVVLSYLLTIHLLGIG 207

Query: 181 MVLVYMETVSSAREVLSKKGLKTSTFAIFLTVSTFASCGFVPTNENMIVFSKNLGLLAML 240
           MVLVYMETVSSAREVLSKKGLKTSTFAIFLTVSTFASCGFVPTNENMIVFSKNLGLLAML
Sbjct: 208 MVLVYMETVSSAREVLSKKGLKTSTFAIFLTVSTFASCGFVPTNENMIVFSKNLGLLAML 267

Query: 241 IPQALLGNTLFPCCLRFCIWSLGKFTKKSETRFLLKNSEEIGYLHLLPRLHSLLLASTVF 300
           IPQALLGNTLFPCCLRF IWSLGKFTKKSETRFLLKNSEEIGYLHLLPRLHSLLLASTVF
Sbjct: 268 IPQALLGNTLFPCCLRFSIWSLGKFTKKSETRFLLKNSEEIGYLHLLPRLHSLLLASTVF 327

Query: 301 GFVLIQSVAIWFMEWSSEALSGLNSIEKLLGVLFLSVNSRHSGESVVDLSMLSPAILFLF 360
           GFVLIQSVAIWFMEWSSEALSGLNSIEKLLGVLFLSVNSRHSGES+VDLSMLSPAILFLF
Sbjct: 328 GFVLIQSVAIWFMEWSSEALSGLNSIEKLLGVLFLSVNSRHSGESIVDLSMLSPAILFLF 387

Query: 361 VAMMYLPPYTSFMPIKGDGEEGENQLEANKIIKKNKRAKFVDNFIFSQISYLLIFIFVIC 420
           VAMMYLPPYTSFMPIKGDGEEGENQLEANKIIKKNKRAKFVDNFIFSQISYLLIFIFVIC
Sbjct: 388 VAMMYLPPYTSFMPIKGDGEEGENQLEANKIIKKNKRAKFVDNFIFSQISYLLIFIFVIC 447

Query: 421 ITERHNIKEDPLNFNLFNIIFEVISAYGNVGFTMGYSCKRQLHPQSNCVDKSFGFSGKWS 480
           ITERHNIKEDPLNFNLFNIIFEVISAYGNVGFTMGYSCKRQLHPQSNCVDKSFGFSGKWS
Sbjct: 448 ITERHNIKEDPLNFNLFNIIFEVISAYGNVGFTMGYSCKRQLHPQSNCVDKSFGFSGKWS 507

Query: 481 SKGKIVLIVVMIFGRLKKFNMNRERAWKL 510
           SKGKIVLIVVMIFGRLKKFNMNRERAWKL
Sbjct: 508 SKGKIVLIVVMIFGRLKKFNMNRERAWKL 536

BLAST of MS002193 vs. NCBI nr
Match: XP_038879237.1 (sodium transporter HKT1-like [Benincasa hispida])

HSP 1 Score: 691.4 bits (1783), Expect = 5.8e-195
Identity = 372/501 (74.25%), Postives = 416/501 (83.03%), Query Frame = 0

Query: 15  FLLLVNPFWIQLFYFLFISAFGLWVLAVLKPKT--YDSYRPANLDLLFTSVSASTVSSMS 74
           FL+ +NPFWIQL YFLFIS+FG ++L VL PKT   +S+ P  LDL FTSVSASTVSSMS
Sbjct: 10  FLIWMNPFWIQLLYFLFISSFGFFILVVLNPKTTSNNSFNPKKLDLFFTSVSASTVSSMS 69

Query: 75  SVEMEVFSDSQLIVLIVLMFVGGEVFTSMVGIQLRKLKLKLSEDKITSVPSDLSTISSNP 134
           +VEMEVFS+SQLIVL +LMF+GGEVFTSMV +QLRK KLK SE KI  V SDLS      
Sbjct: 70  TVEMEVFSNSQLIVLTILMFIGGEVFTSMVELQLRKFKLKFSEQKIACVSSDLS------ 129

Query: 135 QNILSQIELGVVRKLDHQPERQLKHNSIKLLGIVVLSYLLTIHLLGIGMVLVYMETVSSA 194
                 +ELG+VR  +      LK+NSIK LG VVL YLL  + LGIGMVL+Y + VSSA
Sbjct: 130 ------VELGIVRNNNTLDIIILKYNSIKFLGFVVLGYLLIFNFLGIGMVLIYFKFVSSA 189

Query: 195 REVLSKKGLKTSTFAIFLTVSTFASCGFVPTNENMIVFSKNLGLLAMLIPQALLGNTLFP 254
           R+VL +KGLK STF+IFL VSTFASCGFVPTNENMIVF KNLGLL +LIPQAL+GNTLFP
Sbjct: 190 RQVLEQKGLKLSTFSIFLIVSTFASCGFVPTNENMIVFKKNLGLLIILIPQALIGNTLFP 249

Query: 255 CCLRFCIWSLGKFTKKSE--TRFLLKNSEEIGYLHLLPRLHSLLLASTVFGFVLIQSVAI 314
            CLR CIWSLGKF KK +    FLLKNSEEIGYLHLLP+LHSLLL  +V GFVL+Q VAI
Sbjct: 250 SCLRICIWSLGKFMKKKKEIVGFLLKNSEEIGYLHLLPKLHSLLLVGSVLGFVLMQFVAI 309

Query: 315 WFMEWSSEALSGLNSIEKLLGVLFLSVNSRHSGESVVDLSMLSPAILFLFVAMMYLPPYT 374
             MEW S ALSGLN +EKL+G+LFLSVNSRHSGES+VDLS LS AIL LFVAMMYLPPYT
Sbjct: 310 GAMEWRSNALSGLNCVEKLIGILFLSVNSRHSGESIVDLSRLSSAILLLFVAMMYLPPYT 369

Query: 375 SFMPIKGD-GEEGENQ-LEANKIIKKNKRAKFVDNFIFSQISYLLIFIFVICITERHNIK 434
           SFMPIK D GEE ENQ LEANK  +K KRAKF+DNF+FSQ+ YL+IFIF+ICITERHNIK
Sbjct: 370 SFMPIKIDEGEEEENQLLEANKRSRKIKRAKFMDNFVFSQLFYLIIFIFIICITERHNIK 429

Query: 435 EDPLNFNLFNIIFEVISAYGNVGFTMGYSCKRQLHPQSNCVDKSFGFSGKWSSKGKIVLI 494
           EDPLNFN+FNIIFEVISAYGNVGFTMGY+CKRQ+HPQSNC DKSFGFSGKWS  GK+VLI
Sbjct: 430 EDPLNFNIFNIIFEVISAYGNVGFTMGYNCKRQIHPQSNCADKSFGFSGKWSDNGKVVLI 489

Query: 495 VVMIFGRLKKFNMNRERAWKL 510
           VVMIFGRLKKFNMN++R WKL
Sbjct: 490 VVMIFGRLKKFNMNKQRPWKL 498

BLAST of MS002193 vs. NCBI nr
Match: XP_008453695.1 (PREDICTED: cation transporter HKT8-like [Cucumis melo])

HSP 1 Score: 666.0 bits (1717), Expect = 2.6e-187
Identity = 362/507 (71.40%), Postives = 412/507 (81.26%), Query Frame = 0

Query: 15  FLLLVNPFWIQLFYFLFISAFGLWVLAVLKPKTYDSYRPANLDLLFTSVSASTVSSMSSV 74
           FL+L+NPFWI + YFLFIS+FG WVL +L PK      P  LDL FTSVSASTVSSMS++
Sbjct: 10  FLILLNPFWIHVLYFLFISSFGFWVLLILNPK----LTPKKLDLFFTSVSASTVSSMSTM 69

Query: 75  EMEVFSDSQLIVLIVLMFVGGEVFTSMVGIQLRKLKLKLSEDKITSVPSDLSTISSNPQN 134
           EMEVFS+SQLIVL +LMF+GGEVFTSMV +QLRKLKLK SE KI  V +DL+        
Sbjct: 70  EMEVFSNSQLIVLTILMFIGGEVFTSMVELQLRKLKLKFSEKKIACVENDLN-------- 129

Query: 135 ILSQIELGVVRKLDHQPERQLKHNSIKLLGIVVLSYLLTIHLLGIGMVLVYMETVSS-AR 194
             S +ELG +   DH     LK+NSIK LG VVL YLL  + LGI MVLVY++ VSS A 
Sbjct: 130 --SSLELGDID--DHNNFDILKYNSIKFLGYVVLGYLLIFNFLGIVMVLVYLKCVSSTAT 189

Query: 195 EVLSKKGLKTSTFAIFLTVSTFASCGFVPTNENMIVFSKNLGLLAMLIPQALLGNTLFPC 254
           EVL  KGLKTSTF++F+ VSTFASCGFVPTNENMIVF KNLGLL +LIPQAL+GNTLFP 
Sbjct: 190 EVLKSKGLKTSTFSVFVIVSTFASCGFVPTNENMIVFRKNLGLLIILIPQALIGNTLFPS 249

Query: 255 CLRFCIWSLGKFTKKSE--TRFLLKNSEEIGYLHLLPRLHSLLLASTVFGFVLIQSVAIW 314
           CLRFCIWSLGK  KK +    FLL+NSEEIGYLHLLP+L+SL+L ++VFGFVLIQSVAI 
Sbjct: 250 CLRFCIWSLGKIMKKKKEVVGFLLENSEEIGYLHLLPKLYSLVLVASVFGFVLIQSVAIG 309

Query: 315 FMEW---SSEALSGLNSIEKLLGVLFLSVNSRHSGESVVDLSMLSPAILFLFVAMMYLPP 374
            MEW   S+   SGLN +EKL+G+LFLSVNSRHSGES+VDLS LS AIL LFVAMMYLPP
Sbjct: 310 AMEWRSSSNNGFSGLNWVEKLIGILFLSVNSRHSGESIVDLSTLSSAILVLFVAMMYLPP 369

Query: 375 YTSFMPIK------GDGEEGENQLEANKIIKKNKRAKFVDNFIFSQISYLLIFIFVICIT 434
           YTSFMPIK      G+GEE    +E NK  +K KRAKFVDNF+FSQ+SYL+IFIF+ICIT
Sbjct: 370 YTSFMPIKIEEGEEGEGEEENEIMEGNKKRRKTKRAKFVDNFVFSQLSYLIIFIFIICIT 429

Query: 435 ERHNIKEDPLNFNLFNIIFEVISAYGNVGFTMGYSCKRQLHPQSNCVDKSFGFSGKWSSK 494
           ERHNIK+DPLNFN+FNI+FEVISAYGNVGF+MGYSC RQ+HPQSNCVD SFGFSGKWS  
Sbjct: 430 ERHNIKQDPLNFNIFNIVFEVISAYGNVGFSMGYSCGRQVHPQSNCVDMSFGFSGKWSDN 489

Query: 495 GKIVLIVVMIFGRLKKFNMNRERAWKL 510
           GK+VLIVVMIFGRLKKFNMNR+RAWKL
Sbjct: 490 GKLVLIVVMIFGRLKKFNMNRQRAWKL 500

BLAST of MS002193 vs. NCBI nr
Match: XP_004150749.3 (sodium transporter HKT1 [Cucumis sativus])

HSP 1 Score: 644.0 bits (1660), Expect = 1.1e-180
Identity = 352/504 (69.84%), Postives = 410/504 (81.35%), Query Frame = 0

Query: 13  FRFLLLVNPFWIQLFYFLFISAFGLWVLAVLKPKTYDSYRPANLDLLFTSVSASTVSSMS 72
           F FL+L+NPFWI + YFLFIS+FG WVL +L PK      P  LDL FTSVSASTVSSMS
Sbjct: 8   FCFLILLNPFWIHVLYFLFISSFGFWVLLILNPK----LAPKKLDLFFTSVSASTVSSMS 67

Query: 73  SVEMEVFSDSQLIVLIVLMFVGGEVFTSMVGIQLRKLKLKLSEDKITSVPSDLSTISSNP 132
           ++EMEVFS+SQLIVL VLMF+GGEVFTSMV +QLRKLKLK SE KI  V +D +      
Sbjct: 68  TMEMEVFSNSQLIVLTVLMFIGGEVFTSMVELQLRKLKLKFSEQKIACVENDFNN----- 127

Query: 133 QNILSQIELGVVRKLDHQPERQLKHNSIKLLGIVVLSYLLTIHLLGIGMVLVYMETVSS- 192
               + +ELGV+   ++  +  LK++SIK LG VVL YLL  + LGI +VL+Y++ VSS 
Sbjct: 128 ----NNLELGVI---NNSFDIILKYDSIKFLGYVVLGYLLIFNFLGIALVLIYLKRVSST 187

Query: 193 AREVLSKKGLKTSTFAIFLTVSTFASCGFVPTNENMIVFSKNLGLLAMLIPQALLGNTLF 252
           AREVL  KGL+TSTF++F+ VSTFASCGFVPTNENMIVF KNLGLL +LIPQAL+GNTLF
Sbjct: 188 AREVLKSKGLRTSTFSLFVVVSTFASCGFVPTNENMIVFRKNLGLLLILIPQALIGNTLF 247

Query: 253 PCCLRFCIWSLGKFTKKSE--TRFLLKNSEEIGYLHLLPRLHSLLLASTVFGFVLIQSVA 312
           P CLRFCIWSLGK  KK +    FLL+NSEEIGYLHLLP+L+SL+L  +V GFVLIQ VA
Sbjct: 248 PSCLRFCIWSLGKIMKKKKEVIGFLLENSEEIGYLHLLPKLYSLVLVGSVLGFVLIQFVA 307

Query: 313 IWFMEW--SSEALSGLNSIEKLLGVLFLSVNSRHSGESVVDLSMLSPAILFLFVAMMYLP 372
           I  MEW  S+   SGLN +EKL+G+LFLSVNSRHSGES VDLS LS AIL LFVAMMYLP
Sbjct: 308 IGAMEWRSSNNGFSGLNWVEKLIGILFLSVNSRHSGESTVDLSTLSSAILVLFVAMMYLP 367

Query: 373 PYTSFMPIKGDGEEGENQLEAN-KIIKKNKRAKFVDNFIFSQISYLLIFIFVICITERHN 432
           PYTSFMPIK   EE EN++E N K   + KRAKF+DNF+FSQ+SYL+IF F+ICITERHN
Sbjct: 368 PYTSFMPIKSI-EEEENEMEGNRKRRSRTKRAKFMDNFVFSQLSYLIIFTFIICITERHN 427

Query: 433 IKEDPLNFNLFNIIFEVISAYGNVGFTMGYSCKRQLHPQSNCVDKSFGFSGKWSSKGKIV 492
           +K+DPLNFN+FNI+FEVISAYGNVGF+MGYSC R++HPQSNCVDKSFGFSGKWS  GK+V
Sbjct: 428 MKQDPLNFNIFNIVFEVISAYGNVGFSMGYSCGRRVHPQSNCVDKSFGFSGKWSDNGKLV 487

Query: 493 LIVVMIFGRLKKFNMNR-ERAWKL 510
           LIVVMIFGRLKKFNMNR +RAWKL
Sbjct: 488 LIVVMIFGRLKKFNMNRSQRAWKL 494

BLAST of MS002193 vs. NCBI nr
Match: XP_023516746.1 (cation transporter HKT8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 622.9 bits (1605), Expect = 2.5e-174
Identity = 336/500 (67.20%), Postives = 390/500 (78.00%), Query Frame = 0

Query: 15  FLLLVNPFWIQLFYFLFISAFGLWVLAVLKPKT--YDSYRPANLDLLFTSVSASTVSSMS 74
           FL+ V+PFWI LFYFLFIS+ G   L VLKPKT  Y  + P NLDL FTSVSA+TVSSMS
Sbjct: 10  FLIRVHPFWIHLFYFLFISSLGFLALLVLKPKTGSYHPFHPQNLDLFFTSVSAATVSSMS 69

Query: 75  SVEMEVFSDSQLIVLIVLMFVGGEVFTSMVGIQLRKLKLKLSEDKITSVPSDLSTISSNP 134
           +VEME FSD QLIVL VLMF+GGEVFTSMV +Q RK  LK  + KI SVP DL       
Sbjct: 70  AVEMEAFSDPQLIVLSVLMFIGGEVFTSMVALQFRKFNLKFPDLKIASVPIDLEA----- 129

Query: 135 QNILSQIELGVVRKLDHQPERQLKHNSIKLLGIVVLSYLLTIHLLGIGMVLVYMETVSSA 194
                        K D  P  +LK+ SIK LG VV  YL+  + LGI MVL+Y++   +A
Sbjct: 130 -------------KFDPPPPERLKYKSIKFLGFVVFGYLILSNFLGIVMVLIYLKLAPTA 189

Query: 195 REVLSKKGLKTSTFAIFLTVSTFASCGFVPTNENMIVFSKNLGLLAMLIPQALLGNTLFP 254
           R+VL +KGLKT+TF++F+ VSTFASCGFVPTNENMIVF KNLGLL +LIPQALLGNTLFP
Sbjct: 190 RDVLKQKGLKTTTFSVFVVVSTFASCGFVPTNENMIVFRKNLGLLLVLIPQALLGNTLFP 249

Query: 255 CCLRFCIWSLGKFTKKSETR---FLLKNSEEIGYLHLLPRLHSLLLASTVFGFVLIQSVA 314
            CLR  IW LGK  KK + +   FLL+NSEEIG+LHLLP+L S LL  +V GFV++Q VA
Sbjct: 250 SCLRASIWGLGKVVKKKKLKIVGFLLENSEEIGFLHLLPKLDSWLLVGSVCGFVVVQVVA 309

Query: 315 IWFMEWSSEALSGLNSIEKLLGVLFLSVNSRHSGESVVDLSMLSPAILFLFVAMMYLPPY 374
           I  MEWSS ALSGLN  EKL+G+LFLSVNSRHSGE+++DLS+LSPAIL LF AMMYLPPY
Sbjct: 310 IGAMEWSSGALSGLNCGEKLVGILFLSVNSRHSGEAILDLSILSPAILVLFAAMMYLPPY 369

Query: 375 TSFMPIKGDGEEGENQLEANKIIKKNKRAKFVDNFIFSQISYLLIFIFVICITERHNIKE 434
           TSFMPIK D  EGE ++         KRAKF+DNF+FSQ+ YL+IF+F+IC+TERHNIK 
Sbjct: 370 TSFMPIKID--EGERRI---------KRAKFMDNFLFSQLFYLIIFVFIICVTERHNIKH 429

Query: 435 DPLNFNLFNIIFEVISAYGNVGFTMGYSCKRQLHPQSNCVDKSFGFSGKWSSKGKIVLIV 494
           DPLNF+LFN+IFEVISAYGNVGF+MGYSCKRQL+P S CVDKSFGFSGKWS  GK+VLIV
Sbjct: 430 DPLNFSLFNVIFEVISAYGNVGFSMGYSCKRQLYPSSVCVDKSFGFSGKWSDNGKLVLIV 480

Query: 495 VMIFGRLKKFNMNRERAWKL 510
           VMIFGRLKKFNMN++RAWKL
Sbjct: 490 VMIFGRLKKFNMNKQRAWKL 480

BLAST of MS002193 vs. ExPASy Swiss-Prot
Match: Q6H501 (Probable cation transporter HKT6 OS=Oryza sativa subsp. japonica OX=39947 GN=HKT6 PE=2 SV=2)

HSP 1 Score: 423.7 bits (1088), Expect = 2.9e-117
Identity = 240/502 (47.81%), Postives = 332/502 (66.14%), Query Frame = 0

Query: 13  FRFLLLVNPFWIQLFYFLFISAFGLWVLAVLKPKTYDSYRPANLDLLFTSVSASTVSSMS 72
           F+F+   NP ++ + YF  IS  G   L VLKP+   S    +LD+LFTSVSASTVSSM+
Sbjct: 38  FQFIYQNNPLFVHVAYFALISFAGYGSLKVLKPRD-KSNTLKDLDVLFTSVSASTVSSMA 97

Query: 73  SVEMEVFSDSQLIVLIVLMFVGGEVFTSMVGIQLRKLKLKLSEDKITSVPS---DLSTIS 132
           +VEME FS +QL VL +LM +GGEVFTSM+GI   + +    E   T   S   D+ +I+
Sbjct: 98  TVEMEDFSSAQLWVLTILMLIGGEVFTSMLGIHFMRAEFGTKESVSTRDHSPCIDIESIT 157

Query: 133 SNPQNILSQIELGVVRKLDHQPER--QLKHNSIKLLGIVVLSYLLTIHLLGIGMVLVYME 192
           S      +Q     V   + + E    ++  +IK LG VV+ YLL  +L G  ++ +Y+ 
Sbjct: 158 STKFGPSTQGTKVTVSFSELRMENGGHVEPKTIKFLGFVVMGYLLITNLGGSLLIYLYLN 217

Query: 193 TVSSAREVLSKKGLKTSTFAIFLTVSTFASCGFVPTNENMIVFSKNLGLLAMLIPQALLG 252
            V SA ++L +KG+    F++F  +S+  +CGF P NENMI+F KN  LL +++PQ L G
Sbjct: 218 LVPSAHKILKRKGIGIIVFSVFTAISSVGNCGFTPVNENMIIFQKNSILLLLILPQILAG 277

Query: 253 NTLFPCCLRFCIWSLGKFTKKSETRFLLKNSEEIGYLHLLPRLHSLLLASTVFGFVLIQS 312
           NTLF  CLR  +WSL K T K + R++L+  + IGY HL+    S+ L  TV   +++Q+
Sbjct: 278 NTLFAPCLRLMVWSLEKITGKKDCRYILEYPKAIGYKHLMSTRESVYLTLTVVSLIILQT 337

Query: 313 VAIWFMEWSSEALSGLNSIEKLLGVLFLSVNSRHSGESVVDLSMLSPAILFLFVAMMYLP 372
           V    +EWSS AL G+++ +K++  LF SVN+RH+GESV DLS LS AIL L+  MMYLP
Sbjct: 338 VLFLSLEWSSVALDGMSNYQKIVSALFQSVNARHAGESVTDLSNLSSAILVLYTIMMYLP 397

Query: 373 PYTSFMPIKGDGEEGENQLEANKIIKKNKRAKFVDNFIFSQISYLLIFIFVICITERHNI 432
            YTSF+P + DGE+ + +       K NKR   ++N+IFS +SYL IF+ +ICITER ++
Sbjct: 398 GYTSFLP-RHDGEDSKTE-------KINKRKGLLENWIFSHMSYLAIFVMLICITERDSM 457

Query: 433 KEDPLNFNLFNIIFEVISAYGNVGFTMGYSCKRQLHPQSNCVDKSFGFSGKWSSKGKIVL 492
             DPLNFN+F+I+FEV+SAYGNVGF++GYSCKR L+  + C D S+GF+GKWS  GK +L
Sbjct: 458 ATDPLNFNVFSILFEVVSAYGNVGFSVGYSCKRLLNHDARCKDASYGFAGKWSDNGKAIL 517

Query: 493 IVVMIFGRLKKFNMNRERAWKL 510
           I+VM+FGRLK FNM   RAWKL
Sbjct: 518 IIVMLFGRLKTFNMKGGRAWKL 530

BLAST of MS002193 vs. ExPASy Swiss-Prot
Match: Q84TI7 (Sodium transporter HKT1 OS=Arabidopsis thaliana OX=3702 GN=HKT1 PE=1 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 9.5e-116
Identity = 244/494 (49.39%), Postives = 326/494 (65.99%), Query Frame = 0

Query: 22  FWIQLFYFLFISAFGLWVLAVLKPKTYDSYRPANLDLLFTSVSASTVSSMSSVEMEVFSD 81
           F++   YFLF S  G   L + KP+T  + RP + DL FTSVSA TVSSMS+V+MEVFS+
Sbjct: 22  FFLYFIYFLFFSFLGFLALKITKPRT--TSRPHDFDLFFTSVSAITVSSMSTVDMEVFSN 81

Query: 82  SQLIVLIVLMFVGGEVFTSMVGIQLRKL-KLKLSEDKITSVPSDLSTISSNPQNILSQIE 141
           +QLI L +LMF+GGE+FTS + + +    K     +KI  +    ++ SS    I  + +
Sbjct: 82  TQLIFLTILMFLGGEIFTSFLNLYVSYFTKFVFPHNKIRHILGSYNSDSS----IEDRCD 141

Query: 142 LGVVRKLDHQPERQLKHNSIKLLGIVVLSYLLTIHLLGIGMVLVYMETVSSAREVLSKKG 201
           +  V     +   ++   + K L  VVLSY L  +L+G  ++LVY+  V +AR+VLS K 
Sbjct: 142 VETVTDY-REGLIKIDERASKCLYSVVLSYHLVTNLVGSVLLLVYVNFVKTARDVLSSKE 201

Query: 202 LKTSTFAIFLTVSTFASCGFVPTNENMIVFSKNLGLLAMLIPQALLGNTLFPCCLRFCIW 261
           +   TF++F TVSTFA+CGFVPTNENMI+F KN GL+ +LIPQ L+GNTLFPC L   IW
Sbjct: 202 ISPLTFSVFTTVSTFANCGFVPTNENMIIFRKNSGLIWLLIPQVLMGNTLFPCFLVLLIW 261

Query: 262 SLGKFTKKSETRFLLKNSEEIGYLHLLPRLHSLLLASTVFGFVLIQSVAIWFMEWSSEAL 321
            L K TK+ E  ++LKN  ++GY HLL     +LL  TV GF++IQ +     EW+SE+L
Sbjct: 262 GLYKITKRDEYGYILKNHNKMGYSHLLSVRLCVLLGVTVLGFLIIQLLFFCAFEWTSESL 321

Query: 322 SGLNSIEKLLGVLFLSVNSRHSGESVVDLSMLSPAILFLFVAMMYLPPYTSFMPIKG--- 381
            G++S EKL+G LF  VNSRH+GE++VDLS LSPAIL LF+ MMYLPPYT FMP+     
Sbjct: 322 EGMSSYEKLVGSLFQVVNSRHTGETIVDLSTLSPAILVLFILMMYLPPYTLFMPLTEQKT 381

Query: 382 -DGEEGENQLEANKIIKKNKRAKFVDNFIFSQISYLLIFIFVICITERHNIKEDPLNFNL 441
            + E G++  E  K +KK+         I SQ+S+L I IF+I ITER N++ DP+NFN+
Sbjct: 382 IEKEGGDDDSENGKKVKKS-------GLIVSQLSFLTICIFLISITERQNLQRDPINFNV 441

Query: 442 FNIIFEVISAYGNVGFTMGYSCKRQLH-PQSNCVDKSFGFSGKWSSKGKIVLIVVMIFGR 501
            NI  EVISAYGNVGFT GYSC+R++      C D S+GF+G+WS  GK VLI+VM +GR
Sbjct: 442 LNITLEVISAYGNVGFTTGYSCERRVDISDGGCKDASYGFAGRWSPMGKFVLIIVMFYGR 501

Query: 502 LKKFNMNRERAWKL 510
            K+F     RAW L
Sbjct: 502 FKQFTAKSGRAWIL 501

BLAST of MS002193 vs. ExPASy Swiss-Prot
Match: Q93XI5 (Cation transporter HKT2 OS=Oryza sativa subsp. indica OX=39946 GN=HKT2 PE=1 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 1.4e-111
Identity = 238/520 (45.77%), Postives = 332/520 (63.85%), Query Frame = 0

Query: 1   LGKSVCFILVCFFRFLLL-VNPFWIQLFYFLFISAFGLWVLAVLKPKTYDSYRPANLDLL 60
           +G+ V   +V  +RF+ L V+PFWIQL YFL IS  G  +L  LKP +   ++P  +D+L
Sbjct: 19  IGRYVLHSIVLIYRFVSLHVHPFWIQLSYFLLISILGSVLLMFLKPSS-PEFKPGYIDML 78

Query: 61  FTSVSASTVSSMSSVEMEVFSDSQLIVLIVLMFVGGEVFTSMVGIQLR---KLKLKLSED 120
           F S SA TVS +S++EMEV S SQ++VL +LM VGGEVF S +G+ LR   K   + S D
Sbjct: 79  FLSTSAMTVSGLSTIEMEVLSSSQIVVLTLLMLVGGEVFVSFLGLMLRLKHKHNPEFSGD 138

Query: 121 KITSVPSDLSTISSNPQNILSQIELGVVRKLDHQPERQLK--HNSIKL---LGIVVLSYL 180
           +++SVP +L TI    + ++S  EL +       P   +K    S +L   LG VV SY 
Sbjct: 139 RVSSVPIELDTIEPT-RTVMSSEELQIEAAAPDVPSSTIKDLKRSKRLRWFLGFVVFSYF 198

Query: 181 LTIHLLGIGMVLVYMETVSSAREVLSKKGLKTSTFAIFLTVSTFASCGFVPTNENMIVFS 240
           + IH++G  +VL Y+  VSSA+  L KKG+  + F+  +TVS+FA+ G VPTNENM +FS
Sbjct: 199 VVIHVVGFLLVLWYISRVSSAKAPLKKKGINIALFSFSVTVSSFANGGLVPTNENMAIFS 258

Query: 241 KNLGLLAMLIPQALLGNTLFPCCLRFCIWSLGKFTKKSETRFLLKNSEEIGYLHLLPRLH 300
           KN GLL + I Q L GNTL+P  LR  IW LGK TK  + + ++KNS+E+ Y +LLP+L 
Sbjct: 259 KNPGLLLLFIGQILAGNTLYPLFLRILIWFLGKVTKLKDLKLMIKNSDELQYDYLLPKLP 318

Query: 301 SLLLASTVFGFVLIQSVAIWFMEWSSEALSGLNSIEKLLGVLFLSVNSRHSGESVVDLSM 360
           +  LASTV G +         ++W+S    GL+S +K++  LF++VN+RHSGE+ +D S+
Sbjct: 319 TAFLASTVIGLMASLVTLFGSVDWNSSVFDGLSSYQKIINALFMAVNARHSGENSIDCSL 378

Query: 361 LSPAILFLFVAMMYLPPYTSFMPIKGDGEEGENQLEANKIIKKNKRAKFVDNFIFSQISY 420
           ++PA+L LF+ +MYLPP T+F    GD      +  ANK  K+ K    V N  FSQ++ 
Sbjct: 379 IAPAVLVLFIILMYLPPSTTFALSNGD------EKTANKKAKR-KLGLVVRNLAFSQLAC 438

Query: 421 LLIFIFVICITERHNIKEDPLNFNLFNIIFEVISAYGNVGFTMGYSCKR--QLHPQSNCV 480
             +F+ V  ITER  ++ DPLNF+  N+IFEVISAYGNVG T GYSC R  +LHP S C 
Sbjct: 439 NAVFVIVALITERSRLRNDPLNFSALNMIFEVISAYGNVGLTTGYSCSRLQKLHPGSICQ 498

Query: 481 DKSFGFSGKWSSKGKIVLIVVMIFGRLKKFNMNRERAWKL 510
           DK +  SG WS +GK++L+ VM++GRLK F       W+L
Sbjct: 499 DKPYSLSGWWSDEGKLLLVSVMLYGRLKAFTKGTGEYWRL 529

BLAST of MS002193 vs. ExPASy Swiss-Prot
Match: A2YGP9 (Cation transporter HKT1 OS=Oryza sativa subsp. indica OX=39946 GN=HKT1 PE=1 SV=2)

HSP 1 Score: 399.8 bits (1026), Expect = 4.5e-110
Identity = 235/520 (45.19%), Postives = 330/520 (63.46%), Query Frame = 0

Query: 1   LGKSVCFILVCFFRFLLL-VNPFWIQLFYFLFISAFGLWVLAVLKPKTYDSYRPANLDLL 60
           +G+     +V   RF+ L ++PFWIQL YFL IS  G  +L  LKP     +RP  +D+L
Sbjct: 19  IGRYFVNFVVLAHRFIALHIHPFWIQLSYFLLISILGSVLLMFLKPSN-PEFRPGYIDML 78

Query: 61  FTSVSASTVSSMSSVEMEVFSDSQLIVLIVLMFVGGEVFTSMVGIQLR---KLKLKLSED 120
           F S SA T+SS+ ++EMEV S SQ++V+ +LM +GGEVF S +G+ LR   K   + S D
Sbjct: 79  FLSTSALTLSSLITIEMEVLSSSQIVVITLLMLLGGEVFVSFLGLMLRLNHKHNPEFSGD 138

Query: 121 KITSVPSDLSTISSNPQNILSQIELGVVRKLDHQPERQLK--HNSIKL---LGIVVLSYL 180
           K++SVP +L TI+S    ++S  EL +   +   P   +K    S +L   LG VV SY 
Sbjct: 139 KVSSVPIELDTINS-ASTVISCEELQLEAAIPEVPSSTIKDLKRSKRLRWFLGFVVFSYF 198

Query: 181 LTIHLLGIGMVLVYMETVSSAREVLSKKGLKTSTFAIFLTVSTFASCGFVPTNENMIVFS 240
           + IH+ G  +VL Y+  VSSA+  L KKG+  + F+  +TVS+FA+ G VPTNENM +FS
Sbjct: 199 VVIHVAGFLLVLWYISRVSSAKAPLKKKGINIALFSFSVTVSSFANVGLVPTNENMAIFS 258

Query: 241 KNLGLLAMLIPQALLGNTLFPCCLRFCIWSLGKFTKKSETRFLLKNSEEIGYLHLLPRLH 300
           KN GLL + I Q L GNTL+P  LR  IW LGK TK  E + ++KN EE+ Y +LLP+L 
Sbjct: 259 KNPGLLLLFIGQILAGNTLYPLFLRLLIWFLGKVTKLRELKLMIKNPEELQYDYLLPKLP 318

Query: 301 SLLLASTVFGFVLIQSVAIWFMEWSSEALSGLNSIEKLLGVLFLSVNSRHSGESVVDLSM 360
           +  LASTV G +         ++W+S    GL+S +K++  LF++VN+RHSGE+ +D S+
Sbjct: 319 TAFLASTVIGLMASLVTLFGAVDWNSSVFDGLSSYQKIINALFMAVNARHSGENSIDCSL 378

Query: 361 LSPAILFLFVAMMYLPPYTSFMPIKGDGEEGENQLEANKIIKKNKRAKFVDNFIFSQISY 420
           ++PA+L LF+ +MYLPP T+F    GD      +  ANK  K+ K    V N  FSQ++ 
Sbjct: 379 IAPAVLVLFIILMYLPPSTTFALSNGD------EKTANKKAKR-KLGLVVQNLAFSQLAC 438

Query: 421 LLIFIFVICITERHNIKEDPLNFNLFNIIFEVISAYGNVGFTMGYSCKR--QLHPQSNCV 480
           + +F+ V  ITER  ++ DPLNF+  N+IFE+ISAYGNVG + GYSC R  +LHP S C 
Sbjct: 439 ISVFVIVAFITERSRLRNDPLNFSALNMIFEIISAYGNVGLSTGYSCSRLQKLHPGSICQ 498

Query: 481 DKSFGFSGKWSSKGKIVLIVVMIFGRLKKFNMNRERAWKL 510
           DK +  SG WS +GK++L+ VM++GRLK F       W+L
Sbjct: 499 DKPYSLSGWWSDEGKLLLVFVMLYGRLKAFTKGTGEYWRL 529

BLAST of MS002193 vs. ExPASy Swiss-Prot
Match: Q0D9S3 (Cation transporter HKT1 OS=Oryza sativa subsp. japonica OX=39947 GN=HKT1 PE=1 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 4.5e-110
Identity = 235/520 (45.19%), Postives = 330/520 (63.46%), Query Frame = 0

Query: 1   LGKSVCFILVCFFRFLLL-VNPFWIQLFYFLFISAFGLWVLAVLKPKTYDSYRPANLDLL 60
           +G+     +V   RF+ L ++PFWIQL YFL IS  G  +L  LKP     +RP  +D+L
Sbjct: 19  IGRYFVNFVVLAHRFIALHIHPFWIQLSYFLLISILGSVLLMFLKPSN-PEFRPGYIDML 78

Query: 61  FTSVSASTVSSMSSVEMEVFSDSQLIVLIVLMFVGGEVFTSMVGIQLR---KLKLKLSED 120
           F S SA T+SS+ ++EMEV S SQ++V+ +LM +GGEVF S +G+ LR   K   + S D
Sbjct: 79  FLSTSALTLSSLITIEMEVLSSSQIVVITLLMLLGGEVFVSFLGLMLRLNHKHNPEFSGD 138

Query: 121 KITSVPSDLSTISSNPQNILSQIELGVVRKLDHQPERQLK--HNSIKL---LGIVVLSYL 180
           K++SVP +L TI+S    ++S  EL +   +   P   +K    S +L   LG VV SY 
Sbjct: 139 KVSSVPIELDTINS-ASTVISCEELQLEAAIPEVPSSTIKDLKRSKRLRWFLGFVVFSYF 198

Query: 181 LTIHLLGIGMVLVYMETVSSAREVLSKKGLKTSTFAIFLTVSTFASCGFVPTNENMIVFS 240
           + IH+ G  +VL Y+  VSSA+  L KKG+  + F+  +TVS+FA+ G VPTNENM +FS
Sbjct: 199 VVIHVAGFLLVLWYISRVSSAKAPLKKKGINIALFSFSVTVSSFANVGLVPTNENMAIFS 258

Query: 241 KNLGLLAMLIPQALLGNTLFPCCLRFCIWSLGKFTKKSETRFLLKNSEEIGYLHLLPRLH 300
           KN GLL + I Q L GNTL+P  LR  IW LGK TK  E + ++KN EE+ Y +LLP+L 
Sbjct: 259 KNPGLLLLFIGQILAGNTLYPLFLRLLIWFLGKVTKLRELKLMIKNPEELQYDYLLPKLP 318

Query: 301 SLLLASTVFGFVLIQSVAIWFMEWSSEALSGLNSIEKLLGVLFLSVNSRHSGESVVDLSM 360
           +  LASTV G +         ++W+S    GL+S +K++  LF++VN+RHSGE+ +D S+
Sbjct: 319 TAFLASTVIGLMASLVTLFGAVDWNSSVFDGLSSYQKIINALFMAVNARHSGENSIDCSL 378

Query: 361 LSPAILFLFVAMMYLPPYTSFMPIKGDGEEGENQLEANKIIKKNKRAKFVDNFIFSQISY 420
           ++PA+L LF+ +MYLPP T+F    GD      +  ANK  K+ K    V N  FSQ++ 
Sbjct: 379 IAPAVLVLFIILMYLPPSTTFALSNGD------EKTANKKAKR-KLGLVVQNLAFSQLAC 438

Query: 421 LLIFIFVICITERHNIKEDPLNFNLFNIIFEVISAYGNVGFTMGYSCKR--QLHPQSNCV 480
           + +F+ V  ITER  ++ DPLNF+  N+IFE+ISAYGNVG + GYSC R  +LHP S C 
Sbjct: 439 ISVFVIVAFITERSRLRNDPLNFSALNMIFEIISAYGNVGLSTGYSCSRLQKLHPGSICQ 498

Query: 481 DKSFGFSGKWSSKGKIVLIVVMIFGRLKKFNMNRERAWKL 510
           DK +  SG WS +GK++L+ VM++GRLK F       W+L
Sbjct: 499 DKPYSLSGWWSDEGKLLLVFVMLYGRLKAFTKGTGEYWRL 529

BLAST of MS002193 vs. ExPASy TrEMBL
Match: A0A6J1BZS5 (sodium transporter HKT1-like OS=Momordica charantia OX=3673 GN=LOC111006974 PE=3 SV=1)

HSP 1 Score: 962.2 bits (2486), Expect = 8.5e-277
Identity = 505/509 (99.21%), Postives = 507/509 (99.61%), Query Frame = 0

Query: 1   LGKSVCFILVCFFRFLLLVNPFWIQLFYFLFISAFGLWVLAVLKPKTYDSYRPANLDLLF 60
           LGKSVCFILVCFFRFLLL+NPFWIQLFYFLFISAFGLWVLAVLKPKTYDSYRPANLDLLF
Sbjct: 28  LGKSVCFILVCFFRFLLLMNPFWIQLFYFLFISAFGLWVLAVLKPKTYDSYRPANLDLLF 87

Query: 61  TSVSASTVSSMSSVEMEVFSDSQLIVLIVLMFVGGEVFTSMVGIQLRKLKLKLSEDKITS 120
           TSVSASTVSSMSSVEMEVFSDSQLIVLIVLMFVGGEVFTSMVGIQLRKLKLKLSEDKITS
Sbjct: 88  TSVSASTVSSMSSVEMEVFSDSQLIVLIVLMFVGGEVFTSMVGIQLRKLKLKLSEDKITS 147

Query: 121 VPSDLSTISSNPQNILSQIELGVVRKLDHQPERQLKHNSIKLLGIVVLSYLLTIHLLGIG 180
           VPS LSTISSNPQNILSQIELGVVRKLDHQPERQLKHNSIKLLGIVVLSYLLTIHLLGIG
Sbjct: 148 VPSVLSTISSNPQNILSQIELGVVRKLDHQPERQLKHNSIKLLGIVVLSYLLTIHLLGIG 207

Query: 181 MVLVYMETVSSAREVLSKKGLKTSTFAIFLTVSTFASCGFVPTNENMIVFSKNLGLLAML 240
           MVLVYMETVSSAREVLSKKGLKTSTFAIFLTVSTFASCGFVPTNENMIVFSKNLGLLAML
Sbjct: 208 MVLVYMETVSSAREVLSKKGLKTSTFAIFLTVSTFASCGFVPTNENMIVFSKNLGLLAML 267

Query: 241 IPQALLGNTLFPCCLRFCIWSLGKFTKKSETRFLLKNSEEIGYLHLLPRLHSLLLASTVF 300
           IPQALLGNTLFPCCLRF IWSLGKFTKKSETRFLLKNSEEIGYLHLLPRLHSLLLASTVF
Sbjct: 268 IPQALLGNTLFPCCLRFSIWSLGKFTKKSETRFLLKNSEEIGYLHLLPRLHSLLLASTVF 327

Query: 301 GFVLIQSVAIWFMEWSSEALSGLNSIEKLLGVLFLSVNSRHSGESVVDLSMLSPAILFLF 360
           GFVLIQSVAIWFMEWSSEALSGLNSIEKLLGVLFLSVNSRHSGES+VDLSMLSPAILFLF
Sbjct: 328 GFVLIQSVAIWFMEWSSEALSGLNSIEKLLGVLFLSVNSRHSGESIVDLSMLSPAILFLF 387

Query: 361 VAMMYLPPYTSFMPIKGDGEEGENQLEANKIIKKNKRAKFVDNFIFSQISYLLIFIFVIC 420
           VAMMYLPPYTSFMPIKGDGEEGENQLEANKIIKKNKRAKFVDNFIFSQISYLLIFIFVIC
Sbjct: 388 VAMMYLPPYTSFMPIKGDGEEGENQLEANKIIKKNKRAKFVDNFIFSQISYLLIFIFVIC 447

Query: 421 ITERHNIKEDPLNFNLFNIIFEVISAYGNVGFTMGYSCKRQLHPQSNCVDKSFGFSGKWS 480
           ITERHNIKEDPLNFNLFNIIFEVISAYGNVGFTMGYSCKRQLHPQSNCVDKSFGFSGKWS
Sbjct: 448 ITERHNIKEDPLNFNLFNIIFEVISAYGNVGFTMGYSCKRQLHPQSNCVDKSFGFSGKWS 507

Query: 481 SKGKIVLIVVMIFGRLKKFNMNRERAWKL 510
           SKGKIVLIVVMIFGRLKKFNMNRERAWKL
Sbjct: 508 SKGKIVLIVVMIFGRLKKFNMNRERAWKL 536

BLAST of MS002193 vs. ExPASy TrEMBL
Match: A0A1S3BWY9 (cation transporter HKT8-like OS=Cucumis melo OX=3656 GN=LOC103494343 PE=3 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 1.3e-187
Identity = 362/507 (71.40%), Postives = 412/507 (81.26%), Query Frame = 0

Query: 15  FLLLVNPFWIQLFYFLFISAFGLWVLAVLKPKTYDSYRPANLDLLFTSVSASTVSSMSSV 74
           FL+L+NPFWI + YFLFIS+FG WVL +L PK      P  LDL FTSVSASTVSSMS++
Sbjct: 10  FLILLNPFWIHVLYFLFISSFGFWVLLILNPK----LTPKKLDLFFTSVSASTVSSMSTM 69

Query: 75  EMEVFSDSQLIVLIVLMFVGGEVFTSMVGIQLRKLKLKLSEDKITSVPSDLSTISSNPQN 134
           EMEVFS+SQLIVL +LMF+GGEVFTSMV +QLRKLKLK SE KI  V +DL+        
Sbjct: 70  EMEVFSNSQLIVLTILMFIGGEVFTSMVELQLRKLKLKFSEKKIACVENDLN-------- 129

Query: 135 ILSQIELGVVRKLDHQPERQLKHNSIKLLGIVVLSYLLTIHLLGIGMVLVYMETVSS-AR 194
             S +ELG +   DH     LK+NSIK LG VVL YLL  + LGI MVLVY++ VSS A 
Sbjct: 130 --SSLELGDID--DHNNFDILKYNSIKFLGYVVLGYLLIFNFLGIVMVLVYLKCVSSTAT 189

Query: 195 EVLSKKGLKTSTFAIFLTVSTFASCGFVPTNENMIVFSKNLGLLAMLIPQALLGNTLFPC 254
           EVL  KGLKTSTF++F+ VSTFASCGFVPTNENMIVF KNLGLL +LIPQAL+GNTLFP 
Sbjct: 190 EVLKSKGLKTSTFSVFVIVSTFASCGFVPTNENMIVFRKNLGLLIILIPQALIGNTLFPS 249

Query: 255 CLRFCIWSLGKFTKKSE--TRFLLKNSEEIGYLHLLPRLHSLLLASTVFGFVLIQSVAIW 314
           CLRFCIWSLGK  KK +    FLL+NSEEIGYLHLLP+L+SL+L ++VFGFVLIQSVAI 
Sbjct: 250 CLRFCIWSLGKIMKKKKEVVGFLLENSEEIGYLHLLPKLYSLVLVASVFGFVLIQSVAIG 309

Query: 315 FMEW---SSEALSGLNSIEKLLGVLFLSVNSRHSGESVVDLSMLSPAILFLFVAMMYLPP 374
            MEW   S+   SGLN +EKL+G+LFLSVNSRHSGES+VDLS LS AIL LFVAMMYLPP
Sbjct: 310 AMEWRSSSNNGFSGLNWVEKLIGILFLSVNSRHSGESIVDLSTLSSAILVLFVAMMYLPP 369

Query: 375 YTSFMPIK------GDGEEGENQLEANKIIKKNKRAKFVDNFIFSQISYLLIFIFVICIT 434
           YTSFMPIK      G+GEE    +E NK  +K KRAKFVDNF+FSQ+SYL+IFIF+ICIT
Sbjct: 370 YTSFMPIKIEEGEEGEGEEENEIMEGNKKRRKTKRAKFVDNFVFSQLSYLIIFIFIICIT 429

Query: 435 ERHNIKEDPLNFNLFNIIFEVISAYGNVGFTMGYSCKRQLHPQSNCVDKSFGFSGKWSSK 494
           ERHNIK+DPLNFN+FNI+FEVISAYGNVGF+MGYSC RQ+HPQSNCVD SFGFSGKWS  
Sbjct: 430 ERHNIKQDPLNFNIFNIVFEVISAYGNVGFSMGYSCGRQVHPQSNCVDMSFGFSGKWSDN 489

Query: 495 GKIVLIVVMIFGRLKKFNMNRERAWKL 510
           GK+VLIVVMIFGRLKKFNMNR+RAWKL
Sbjct: 490 GKLVLIVVMIFGRLKKFNMNRQRAWKL 500

BLAST of MS002193 vs. ExPASy TrEMBL
Match: A0A6J1E0B9 (probable cation transporter HKT6 OS=Cucurbita moschata OX=3662 GN=LOC111429681 PE=3 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 7.9e-174
Identity = 334/500 (66.80%), Postives = 390/500 (78.00%), Query Frame = 0

Query: 15  FLLLVNPFWIQLFYFLFISAFGLWVLAVLKPKT--YDSYRPANLDLLFTSVSASTVSSMS 74
           FL+ V+PFWI LFYFL IS FG   L VLKPKT  YD + P NLDL FTSVSA+TVSSMS
Sbjct: 10  FLIRVHPFWIHLFYFLSISCFGFLALLVLKPKTASYDHFHPQNLDLFFTSVSAATVSSMS 69

Query: 75  SVEMEVFSDSQLIVLIVLMFVGGEVFTSMVGIQLRKLKLKLSEDKITSVPSDLSTISSNP 134
           +VEME FSD QLIVL VLMF+GGEVFTSMV +Q RK  LK  + KI SVPSDL       
Sbjct: 70  AVEMEAFSDPQLIVLSVLMFIGGEVFTSMVALQFRKFNLKFPDHKIASVPSDLEA----- 129

Query: 135 QNILSQIELGVVRKLDHQPERQLKHNSIKLLGIVVLSYLLTIHLLGIGMVLVYMETVSSA 194
                        K D  P  +LK+ SIK LG VV  YL+  + LGI MVL+Y++   +A
Sbjct: 130 -------------KFDPPPPERLKYKSIKFLGFVVFGYLILFNFLGIVMVLIYLKLAPTA 189

Query: 195 REVLSKKGLKTSTFAIFLTVSTFASCGFVPTNENMIVFSKNLGLLAMLIPQALLGNTLFP 254
           R+VL +KGLKT+TF++F+ VSTFASCGFVPTNENMIVF KNLGLL +LIPQALLGNTLFP
Sbjct: 190 RDVLKQKGLKTTTFSVFVVVSTFASCGFVPTNENMIVFRKNLGLLLVLIPQALLGNTLFP 249

Query: 255 CCLRFCIWSLGKFTKKSETR---FLLKNSEEIGYLHLLPRLHSLLLASTVFGFVLIQSVA 314
            CLR  IW LGK  KK + +   FLL+NSEEIG+LHLLP+L S LL  +V GFV++Q VA
Sbjct: 250 SCLRASIWGLGKVVKKKKLKIVGFLLENSEEIGFLHLLPKLDSWLLVGSVCGFVVVQVVA 309

Query: 315 IWFMEWSSEALSGLNSIEKLLGVLFLSVNSRHSGESVVDLSMLSPAILFLFVAMMYLPPY 374
           I  MEWSS ALSGLN  EKL+G+LFLSVNSRHSGE++VDLS+LSPAIL LF AMMYLPPY
Sbjct: 310 IGAMEWSSGALSGLNCGEKLVGILFLSVNSRHSGEAIVDLSILSPAILVLFAAMMYLPPY 369

Query: 375 TSFMPIKGDGEEGENQLEANKIIKKNKRAKFVDNFIFSQISYLLIFIFVICITERHNIKE 434
           TSFMPIK D  EG+ ++         KRAKF+DNF+FSQ+ YL++F+F+IC+TER NIK+
Sbjct: 370 TSFMPIKID--EGDGRI---------KRAKFMDNFLFSQLFYLIMFVFIICVTERRNIKD 429

Query: 435 DPLNFNLFNIIFEVISAYGNVGFTMGYSCKRQLHPQSNCVDKSFGFSGKWSSKGKIVLIV 494
           D LNF+LFNIIFEVISAYGNVGF++GYSCKRQL+P S CVDKSFGF+GKWS  GK+VLIV
Sbjct: 430 DSLNFSLFNIIFEVISAYGNVGFSLGYSCKRQLYPSSVCVDKSFGFAGKWSDNGKLVLIV 480

Query: 495 VMIFGRLKKFNMNRERAWKL 510
           VMIFGRLKKFNMN++RAWKL
Sbjct: 490 VMIFGRLKKFNMNKQRAWKL 480

BLAST of MS002193 vs. ExPASy TrEMBL
Match: A0A4Y1QLH0 (High-affinity K+ transporter 1 OS=Prunus dulcis OX=3755 GN=Prudu_000490 PE=3 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 6.5e-168
Identity = 322/515 (62.52%), Postives = 397/515 (77.09%), Query Frame = 0

Query: 1   LGKSVCFILVCFFRFLL-LVNPFWIQLFYFLFISAFGLWVLAVLKPKTYDSYRPANLDLL 60
           L KS+ F+LVC++RF+L    PFWI+L YF+ +S  G WVL  LKP+T  S+RP NLDL 
Sbjct: 25  LSKSMGFLLVCYYRFVLRRAKPFWIELVYFVTLSFLGFWVLKALKPRTDPSFRPRNLDLF 84

Query: 61  FTSVSASTVSSMSSVEMEVFSDSQLIVLIVLMFVGGEVFTSMVGIQLRKLKLK-LSEDKI 120
           FTSVSASTVSSMS+VEMEVFS++QLI+L +LMF+GGEVFTSM G+QL K KLK L+++K+
Sbjct: 85  FTSVSASTVSSMSTVEMEVFSNAQLIILTILMFIGGEVFTSMFGLQLWKFKLKLLTKEKV 144

Query: 121 TSVPSDLSTISSNPQNIL-SQIELG-VVRKLDHQPERQLKHNSIKLLGIVVLSYLLTIHL 180
            S  SD+S  S+   NI+   +ELG +  K + Q    LK NSIK LG VVL YLL IH 
Sbjct: 145 ASASSDISPSSTPTNNIVFDHVELGSLASKPEFQDGESLKFNSIKFLGCVVLGYLLVIHG 204

Query: 181 LGIGMVLVYMETVSSAREVLSKKGLKTSTFAIFLTVSTFASCGFVPTNENMIVFSKNLGL 240
           LG+ MV +Y+  +SSAR VL  KGL   TF+ F TVSTF+SCGFVPTNENM+VFSKN GL
Sbjct: 205 LGVIMVSLYLAVISSARNVLKNKGLNMHTFSFFTTVSTFSSCGFVPTNENMMVFSKNSGL 264

Query: 241 LAMLIPQALLGNTLFPCCLRFCIWSLGKFTKKSE--TRFLLKNSEEIGYLHLLPRLHSLL 300
           L +LIPQ LLGNTL P CLRF IW LGK  KK E    +LLKN+ ++G+LHLLP LHS L
Sbjct: 265 LLILIPQVLLGNTLLPSCLRFSIWVLGKIFKKKEYLCSYLLKNTRKLGFLHLLPSLHSSL 324

Query: 301 LASTVFGFVLIQSVAIWFMEWSSEALSGLNSIEKLLGVLFLSVNSRHSGESVVDLSMLSP 360
           L  TVFGF+++Q VA   +EWSS++LSGLNS +K++G LF SVNSRH+GE++VDLS++SP
Sbjct: 325 LVVTVFGFIMVQLVAFLSLEWSSDSLSGLNSYQKIIGALFQSVNSRHTGETIVDLSIISP 384

Query: 361 AILFLFVAMMYLPPYTSFMPIKGDGEEGENQLEANKIIKKNKRAKFVDNFIFSQISYLLI 420
           AIL LFV MMYLPPYTSF+PIK D    +N L+ ++  +K +R K V+N IFSQ+SYL I
Sbjct: 385 AILVLFVVMMYLPPYTSFLPIKDD---DQNPLQPHE-RRKTRRGKVVENLIFSQLSYLAI 444

Query: 421 FIFVICITERHNIKEDPLNFNLFNIIFEVISAYGNVGFTMGYSCKRQLHPQSNCVDKSFG 480
           FI ++CI ER  +KEDPLNFN+ NI+ EVISAYGNVGFT GYSCKRQ+HP  +C DK +G
Sbjct: 445 FIVLVCIIERKQMKEDPLNFNVLNIVIEVISAYGNVGFTTGYSCKRQIHPNGDCQDKWYG 504

Query: 481 FSGKWSSKGKIVLIVVMIFGRLKKFNMNRERAWKL 510
           FSG+WS +GK+VLI VM FGRLKKFNM+  RAWKL
Sbjct: 505 FSGRWSDQGKLVLIAVMFFGRLKKFNMDGGRAWKL 535

BLAST of MS002193 vs. ExPASy TrEMBL
Match: M5XNP4 (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G066800 PE=3 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 1.3e-163
Identity = 321/536 (59.89%), Postives = 396/536 (73.88%), Query Frame = 0

Query: 1   LGKSVCFILVCFFRFLL-LVNPFWIQLFYFLFISAFGLWVLAVLKPKTYDSYRPANLDLL 60
           L K + F+LVC++RF+L    PFWI+L YF+ +S  G WVL  LKP+T  S+RP NLDL 
Sbjct: 25  LSKPMGFLLVCYYRFVLRRAKPFWIELVYFVTLSFLGFWVLKALKPRTDPSFRPRNLDLF 84

Query: 61  FTSVSASTVSSMSSVEMEVFSDSQLIVLIVLMFVGGEVFTSMVGIQLRKLKLK-LSEDKI 120
           FTSVSASTVSSMS+VEMEVFS++QLI+L +LMF+GGEVFTSMVG+QL KLKL  L+++K+
Sbjct: 85  FTSVSASTVSSMSTVEMEVFSNAQLIILTILMFIGGEVFTSMVGLQLWKLKLMLLTKEKV 144

Query: 121 TSVPSDLSTISSNPQNIL-SQIELGVVRKLDHQPERQ----------------------L 180
            S  SD+S  S+   NI+   +ELG    L  +PE Q                      L
Sbjct: 145 ASASSDISPSSTPTNNIVFDHVELG---SLASKPEFQDLNLGVEVYDQDQIDKPLDGESL 204

Query: 181 KHNSIKLLGIVVLSYLLTIHLLGIGMVLVYMETVSSAREVLSKKGLKTSTFAIFLTVSTF 240
           K NSIK LG VV+ YLL IH LG+ MV +Y+  +SSAR +L  K L   TF+ F TVSTF
Sbjct: 205 KFNSIKFLGCVVVGYLLVIHGLGVLMVSLYLAVISSARNILKNKELNMHTFSFFTTVSTF 264

Query: 241 ASCGFVPTNENMIVFSKNLGLLAMLIPQALLGNTLFPCCLRFCIWSLGKFTKKSE--TRF 300
           +SCGFVPTNENM+VFSKN GLL +LIPQ LLGNTL P CLRF IW LGK  KK E    +
Sbjct: 265 SSCGFVPTNENMMVFSKNTGLLLILIPQVLLGNTLLPSCLRFSIWVLGKIFKKKEHLCNY 324

Query: 301 LLKNSEEIGYLHLLPRLHSLLLASTVFGFVLIQSVAIWFMEWSSEALSGLNSIEKLLGVL 360
           LLKN+ ++G+LHLLP LHS LL  TV GF+++Q VA   +EWSS++LSGLNS +K++G L
Sbjct: 325 LLKNTRKLGFLHLLPSLHSSLLVVTVLGFIMVQLVAFLSLEWSSDSLSGLNSYQKIIGAL 384

Query: 361 FLSVNSRHSGESVVDLSMLSPAILFLFVAMMYLPPYTSFMPIKGDGEEGENQLEANKIIK 420
           F SVNSRH+GE++VDLS++SPAIL LFV MMYLPPYTSF+PIK D    +N L+  +  +
Sbjct: 385 FQSVNSRHTGETIVDLSIISPAILVLFVVMMYLPPYTSFLPIKDD---DQNPLQPQE-RR 444

Query: 421 KNKRAKFVDNFIFSQISYLLIFIFVICITERHNIKEDPLNFNLFNIIFEVISAYGNVGFT 480
           K +R K V+N IFSQ+SYL IFI ++CITER  +KEDPLNFN+ NI+ EVISAYGNVGFT
Sbjct: 445 KTRRGKVVENLIFSQLSYLAIFIVLVCITERKQMKEDPLNFNVLNIVIEVISAYGNVGFT 504

Query: 481 MGYSCKRQLHPQSNCVDKSFGFSGKWSSKGKIVLIVVMIFGRLKKFNMNRERAWKL 510
            GYSCKRQ+HP  +C DK +GFSG+WS +GK+VLI VM FGRLKKFNM+  RAWKL
Sbjct: 505 TGYSCKRQIHPNGDCQDKWYGFSGRWSDQGKLVLIAVMFFGRLKKFNMDGGRAWKL 553

BLAST of MS002193 vs. TAIR 10
Match: AT4G10310.1 (high-affinity K+ transporter 1 )

HSP 1 Score: 418.7 bits (1075), Expect = 6.7e-117
Identity = 244/494 (49.39%), Postives = 326/494 (65.99%), Query Frame = 0

Query: 22  FWIQLFYFLFISAFGLWVLAVLKPKTYDSYRPANLDLLFTSVSASTVSSMSSVEMEVFSD 81
           F++   YFLF S  G   L + KP+T  + RP + DL FTSVSA TVSSMS+V+MEVFS+
Sbjct: 22  FFLYFIYFLFFSFLGFLALKITKPRT--TSRPHDFDLFFTSVSAITVSSMSTVDMEVFSN 81

Query: 82  SQLIVLIVLMFVGGEVFTSMVGIQLRKL-KLKLSEDKITSVPSDLSTISSNPQNILSQIE 141
           +QLI L +LMF+GGE+FTS + + +    K     +KI  +    ++ SS    I  + +
Sbjct: 82  TQLIFLTILMFLGGEIFTSFLNLYVSYFTKFVFPHNKIRHILGSYNSDSS----IEDRCD 141

Query: 142 LGVVRKLDHQPERQLKHNSIKLLGIVVLSYLLTIHLLGIGMVLVYMETVSSAREVLSKKG 201
           +  V     +   ++   + K L  VVLSY L  +L+G  ++LVY+  V +AR+VLS K 
Sbjct: 142 VETVTDY-REGLIKIDERASKCLYSVVLSYHLVTNLVGSVLLLVYVNFVKTARDVLSSKE 201

Query: 202 LKTSTFAIFLTVSTFASCGFVPTNENMIVFSKNLGLLAMLIPQALLGNTLFPCCLRFCIW 261
           +   TF++F TVSTFA+CGFVPTNENMI+F KN GL+ +LIPQ L+GNTLFPC L   IW
Sbjct: 202 ISPLTFSVFTTVSTFANCGFVPTNENMIIFRKNSGLIWLLIPQVLMGNTLFPCFLVLLIW 261

Query: 262 SLGKFTKKSETRFLLKNSEEIGYLHLLPRLHSLLLASTVFGFVLIQSVAIWFMEWSSEAL 321
            L K TK+ E  ++LKN  ++GY HLL     +LL  TV GF++IQ +     EW+SE+L
Sbjct: 262 GLYKITKRDEYGYILKNHNKMGYSHLLSVRLCVLLGVTVLGFLIIQLLFFCAFEWTSESL 321

Query: 322 SGLNSIEKLLGVLFLSVNSRHSGESVVDLSMLSPAILFLFVAMMYLPPYTSFMPIKG--- 381
            G++S EKL+G LF  VNSRH+GE++VDLS LSPAIL LF+ MMYLPPYT FMP+     
Sbjct: 322 EGMSSYEKLVGSLFQVVNSRHTGETIVDLSTLSPAILVLFILMMYLPPYTLFMPLTEQKT 381

Query: 382 -DGEEGENQLEANKIIKKNKRAKFVDNFIFSQISYLLIFIFVICITERHNIKEDPLNFNL 441
            + E G++  E  K +KK+         I SQ+S+L I IF+I ITER N++ DP+NFN+
Sbjct: 382 IEKEGGDDDSENGKKVKKS-------GLIVSQLSFLTICIFLISITERQNLQRDPINFNV 441

Query: 442 FNIIFEVISAYGNVGFTMGYSCKRQLH-PQSNCVDKSFGFSGKWSSKGKIVLIVVMIFGR 501
            NI  EVISAYGNVGFT GYSC+R++      C D S+GF+G+WS  GK VLI+VM +GR
Sbjct: 442 LNITLEVISAYGNVGFTTGYSCERRVDISDGGCKDASYGFAGRWSPMGKFVLIIVMFYGR 501

Query: 502 LKKFNMNRERAWKL 510
            K+F     RAW L
Sbjct: 502 FKQFTAKSGRAWIL 501

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134789.11.7e-27699.21sodium transporter HKT1-like [Momordica charantia][more]
XP_038879237.15.8e-19574.25sodium transporter HKT1-like [Benincasa hispida][more]
XP_008453695.12.6e-18771.40PREDICTED: cation transporter HKT8-like [Cucumis melo][more]
XP_004150749.31.1e-18069.84sodium transporter HKT1 [Cucumis sativus][more]
XP_023516746.12.5e-17467.20cation transporter HKT8-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q6H5012.9e-11747.81Probable cation transporter HKT6 OS=Oryza sativa subsp. japonica OX=39947 GN=HKT... [more]
Q84TI79.5e-11649.39Sodium transporter HKT1 OS=Arabidopsis thaliana OX=3702 GN=HKT1 PE=1 SV=1[more]
Q93XI51.4e-11145.77Cation transporter HKT2 OS=Oryza sativa subsp. indica OX=39946 GN=HKT2 PE=1 SV=1[more]
A2YGP94.5e-11045.19Cation transporter HKT1 OS=Oryza sativa subsp. indica OX=39946 GN=HKT1 PE=1 SV=2[more]
Q0D9S34.5e-11045.19Cation transporter HKT1 OS=Oryza sativa subsp. japonica OX=39947 GN=HKT1 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1BZS58.5e-27799.21sodium transporter HKT1-like OS=Momordica charantia OX=3673 GN=LOC111006974 PE=3... [more]
A0A1S3BWY91.3e-18771.40cation transporter HKT8-like OS=Cucumis melo OX=3656 GN=LOC103494343 PE=3 SV=1[more]
A0A6J1E0B97.9e-17466.80probable cation transporter HKT6 OS=Cucurbita moschata OX=3662 GN=LOC111429681 P... [more]
A0A4Y1QLH06.5e-16862.52High-affinity K+ transporter 1 OS=Prunus dulcis OX=3755 GN=Prudu_000490 PE=3 SV=... [more]
M5XNP41.3e-16359.89Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G066800 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G10310.16.7e-11749.39high-affinity K+ transporter 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003445Cation transporterPFAMPF02386TrkHcoord: 150..497
e-value: 1.3E-32
score: 113.0
NoneNo IPR availablePANTHERPTHR31064POTASSIUM TRANSPORT PROTEIN DDB_G0292412-RELATEDcoord: 15..509
NoneNo IPR availablePANTHERPTHR31064:SF27SODIUM TRANSPORTER HKT1coord: 15..509

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002193.1MS002193.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
molecular_function GO:0008324 cation transmembrane transporter activity