MS002180 (gene) Bitter gourd (TR) v1

Overview


Name	MS002180
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	tRNA pseudouridine synthase
Location	scaffold30: 3013587 .. 3019877 (+)
RNA-Seq Expression	MS002180
Synteny	MS002180

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATACCAAATCCGAGGGCTAGACTTGTATCAAGTGCCGATTTCGGTTGCAAGTTTCTGATGGAGAATCGAGCTCCGCAAAAGGATTATCTCCATTACAATCACACTGATCGCTGCAGAAATGCTAGATGGACCGCCAGGTTTTTCTTTCGTCCATCTACGTTATCCGCCAGTTTGTTTGATTTGAGATTTCTTTTGAAGTGTTTCGACTAAAATTCTGTTGCGATGAGAACTTCGCTTTTTTTTTTTTTTGTAGTAATTAAATCTGAATATGAGAAGGCTATCGATCAAGCTGTTCTTATGCTTTTGTGCAGGGAAAGCTACCAACTCATGTATGGAAGGCCGTGGCAAGAAGTGCTTGATTTTTATTTTCATGCTGTTAATGGAAGATTCTCTCCGTCCTCATTGTTTGGAACTGAGGTGAAGGTGAGTCTCCAATATCAACGTTTTCTTTAAACTTCTTTCTTCTTTCTGTATAAAGCAACAGCAATGTGAACCAGGACTATGTTTAATGCCACTTTCATTATAATGTTCTAACCGCGTTGGATAAGTTTTAACTACCTGGTGATATTAGTTTCTAGGGTTACTTTCTTTTGCTTCAAGCAAAGCAGTCCGAAGAGTAGTTTAAAACATTTATATGTCTTCTTTTTGCAATCAAGTTAGTAGAAAATAGAGAGGATAATTCATTTGTAGTACGGCTTCCTAAATCCTAAGGAATCGTATTTCTTTTTGTTCACATTATCTGGTCTTTAAAATAATGCTTGCCATCTAATACTTTCAATCGCTGTCACATTTTCCAGTGTGTGTTTGTTCTGAGTTTCGCTTGGATTTGGAGTTCTATTTAATCAATCATGTGCTACCAATTGTTTATTTGATTAGTTGATCATAATCATTTTTTAAATATTGTTTTATTGGTTGATTTGAATTAGGAGAAATGAGTATGAAAATTTTGTTCCATAATTATCTTTCAGCACGAAAGAAGAAGGTATCATTGATTTATTTATTTATTGTGTATAGTACTTCATCCGCAGACAGTTCATGATCATAATGATGCTGAGATTCCATTGATTTACAACACATCAGAGTTAAATAGAGCTTCAGTTAAAGATAAGACTGGGAGGTGGGCAAGGGCAACATTTAAGATTGTTCTCTCCTACCATGGAAATTCTTTTGATGGATGGCAAAAGCAGCCAGGCTTGAATACTGTTCAAGGGTAAGCTTGTTTCCTGAAACCATATGCACAAGTAATAAACATATACAATTGGAAGATTTTGAAGCTAGCGTTGTTCTTTTTAGTTTTTATTATAAAGCTTTTTAAATTGAAGAACCGATAAGTATTTGCAAGTGTTAAAAAAAGTTTGAGAAGTCAGTACTAGAGATACAATTGAATATCCCATACCTCCATTTCGAGTGAAACTTTCCTCTTGAGGACAATGTCCAGATTAGATCAATTGACCATATTAATCTCACCTTTCATGTATCTCCTTCTTCCAGTAGCAGTATAACAGCTTCAAGTAATATCTTAATATCTTTTGATGTATAAATAGTTCGATTTCAGTCTAGAGATGGCAAAGACTCCTGTATATTGGAGGATCACGTCTATTTGCATGGATATCTGCTACTCTTTTTATTAAATTACTTTTGTGTTGCCTCATATCCTTCGGCAGTTTGGTAGAAAAATCTCTTGGGAAATTTGTAGATGAGAAGAAAGCTCAGCTGCTGAAAGATAGATGCTTACCACTAGAAGGATGTGCTGTGGTTGCTGGGCGCACTGACAAAGGGGTGACAGCTCTGCGACAAGTTTGCTCATTCTGTATGTTAAATTTTTAAATCTTCGTTGACATGATTTTGTGCTTGTTTAGATATTTAATTCCACAACTTTGCTTTATTTTTCTTAGATTCTATCTCTAAATAAAAAACGAACTCTTAGATTACAATAAATTTTATCTGTTTATATTATTTCTCGACAACTCTGATAGTTGCTGATGGATAACCATTAATTGCTTTGTCTCATCCGTTTGATATAAACTCCACTTTTGCGGTGCTTCACTAAAACACTCAAATTGATAACTCTGTTCCAACTGCTCTATACCCAAAGAATTGAAAGGTACGCTCAAAATACTTCTTAAAAGTGAGCAGTATTAAAACAGGAGTGGTGCATAGTCTATGTTTAATGTTATATAACTCGTTTCTTGATTAAAACCTCATAATTTTTTTCCTTTTAAATAATCAGTGGGAGGTGCAACACTGGAATTAAATCCAGGAAGGAAATTCCGATAAAAAGTAGCCAGAGTAAGATTTTTAATTTTTAAATTTTTAAAATTTTAATTTTTAATATGAAACCATGCTTTGATTGAGAAAAATGAAAACAAGAAAAACAAACAGCCCAGAGGACGACTACTACAAAAAGGGCTCAAATCCAAAAGAATAACACCTAACTGATAATTACAAAAGGCCCTGCCAAACCCCACTAGGAAGTATTAAACCTAAAGATTCCAAACAAACTCCACGGATCTCTCCACCCCTAAAAGTTATATTGTTGATAATTACAAAAGGCCCTAAAACCCCACTAGGAAGTATTAAACCTAATAAGATCCCAAACAAACTCCATGGATTTCTCCATCCCTAAAAGTTGTATTGTTCCTCTCCGGTCAAATACCTCATAAAGTAGCCAAAAACTTTGTACAACACCCACTGTATCTTAATTAGGCCAATCTTTAGGAGCTTATTGTTTAGAAGGTTTGACCTAGATACCCATCACTGATGATCAATTCAAGTAGTTCTGCAGCATGGAACTTGGAGTGCATTTCTTTATACCCTTTGCAGAAATCCTTATCTGATGCTATATGAAAAGGAAAGTTGGCATAGAAGATTAAATTTTCTCAATGCCAATAATAGAATTCAAGCCAGACATCCAAACTACTTGGACATTTGATGTACAAGGCTATGTTTTATTTTTTCTTTCGACACATTTGTCTCCAGACCATAGTTTTTTTACAATACTTATAACCCTTTATGTTAACTTCTGAATCTTGTCTGAACTGCGTTTCTTTTGGTTTGAGTTCTTTAACATAAATATATGTTATATGTTTCCTTATTAAGAAAAATGCAATTACAACATACCTTTGCTTTTATTTCTTCCACAAAAGTATTTGAGTTATTGCTATTTCTTTCCATCTTTTGTGTCAAATTTCCTTCGGAGGTGAACAGTTCTGTATCTCTTTCTGACTTCATCCACTTTATTTTTATTATTATTTTCTAATTTTAGAGAACCAATATCTGAATTCATTTAAACTCACATCACTTGTAGATACTTGGCGCACGGATGTTAAACCTCTAGACATTGAAGATTCGATCAATAGTGCAGCTCCCGGAAAACTCAGAGTCTTGTCTGTTTCTCAGGTAACATGCTGGTTTTCCATGAAATGTGGCATTGTAAATGAATGTGGTTCCACTGTCTAGCTCTAATATTGTTGCAGAATAACTTGGTGTTTATTTAACTTTCAATTTTGCTTGTCTCTCTCTTTCAAATACATGTAACTGCTCAATAACTTACTGCTGTAGGTGTCTCGTAACTTCCACCCAAATTTCTCTGCTAAATGGAGGCGATATCTGTATATTTTCCCTCTCAAAGATGGAGAGAATATGGAACCAGATTTCGCAATTGGAGAAGACGCAGAAAACTGTAGAGAAGAGGAAAATTATAATAAAATTGAAGCCTTGAATGGTGAATTCAAGGATGAGATTCTGGATAACTTGGTCCTCAGTCAAAATGAGGAGCTTGCATCAGATGATAAGCCTCAAACATTTAGTGTAAGGAGGGTTAACCGACTACTAAGGAAACTAGAAGGAAAGTTATTGTCATACAAGATGTTTGCACGTGACACCAAAGCATCAAGAAATGAGTGAGAACCATAAATTGAATCAACTATTGGCATTTTTTGTAGTTACTATAGCTTCTAGTTTGAGTATCATTATTAATTAGTTTTACTTTTGTTTCAATAATATTTATTATTTAATCCATTCTAATAAAAAAAATATTTGTTTTTAATTCTTATTGACTGTAAATCTTATTCAGTTGAATATAAGTTATTTCTTGGATTTTCTATTTACTAATAGCTTTCGATCAGAAATTTATGTAGATTTCATTGACTCATGTATGTCAAACTTTTGTTTTGCAATTGCGTTGCGTGTTTGACCGTGCCTTCCAATCAATATAAGTTTGACCTTGCTCTTTCTCCTGGCACGTATGGAGGTTTCTTCTAGTAACTTTTGACTTCATCGGTGTCTTCCCTTGATTCTTAAAGATTTGCTTCTGCTTCTATGGGGTCAATTCATTCAAAAAGAAAGCCGAGACTCATGATGGAGGTTATTTTTTCTTGGAAAATTTGGCTTGAAAGAAATAGGGGCAGTGTTCTCAGGAGTTGAGAAATGTTTAGAGGTTATTTGGGAGCTCATATTTTCCAATTCATTTTAGAGTACTAGCATTACAATTTCTGATCATAAAGTCTATCAAGCGTTTTCCTTCTATTCATTAGTCACTTTTTTGAATTTTTTTAGAATCTGTTTATAGGAAGGTGAAAATGGATGAATCCCATCTCTGCTATTTTCTAGGACCTGTGGATAGAAAGGAACAGCAGGTTTTTCTCGAGTAAAAATAAGGAAAAGTAGGAGACATGGTCAATGACAAAGTTAAGCATCAACTTGATATGCTTTGTTCAAGCCTGTGTACTTACTCACCTAATGTAGTCGACTCTTCCTTTAACAGTTTTTTTAGGTCCTTCATTGGGGCTTCTGTCTCCCCTTTTGTACTTTTCATCATATCGATTTCTTTTGATTGTAGATATGATTAGGATAGCTAGTCTGAAGGCTGACTAAACAAAGTCAGTTATAAAGTTTACAAGTCAACTTTTCATTGATGTGATTAATGTGCTTGCTCGTATCTTTTTTCCTCCAACTGTCTTATCAACGACAGGATGATGTTCAGAACTTTCTGACGTGTACTAATGGCAACTATCCTCCTTTGCAGAGGTCCACCTACCGAGTGTTTTCTTTACCATGCACGAGCAGTGGAAGCTAGACTACCATGCTTGGTATTTTCTTACTTATGGTCATCTTCACGTTCTCCTTTTCTTTGATTATCTTTTTCCCTTTTATACTCCTCCCCTTGTTAAAATGAATTTGCAGAGTTGTAACATGTGATTTGCATTGTGTTTTCTACTGGGTCAGAACGTAGAGAACAGAAGATGAATATGTTAAAAAGTGAGTGTGAACCGAGGAAATGTAGAAGAAATTTACTTTGACATTATGTCATCAATGTGAACTCGTGAAGTACTTTAAAGAAAACTAGTTGTAGCATGTGCTCGATTCAATGCAGTTTCAGATTTTATTGACAATGGTTGCTCTCTCTTCTCTCAATATCTAAAATTGGATAACCTAAAAAATTTCAAAGATCATCTTTGAGTCTGAACCTCAACTTGGGAACAATGTGAACTTTAAGTAGAGTGCTTAATTGTTTATTTGGACTGATCTGTGGAACCTATGCTGCAACAGATGCACAAGTCCAAACTATATTGAACCAATATGAAGTTTATAATACGTTATAGAAATTATTGTAATAGAGATTAGGGTTTTGATTCTATGCTAGAAAAGCCAGGTTTTCAGCAAAACTACATCCCCCACAACTTGCGTGAGTGACATGCTTTTGAATGCAGCTACTCCAAATTTAGGTATCTGACTTAGTGGCGGTGTTGTATTTACAGGATCAAGGAGTAGGAAGAAGTGTTATGTGTGTTGAGCTGGTCGCTAATCGCTTCTTGCGAAAGGTATCATCTCCAGGCACCTATGCTAAAGAGTATATTGTACTGAACATAAAATTTGTTTATTTATATCAAAATCTTTCATTCCAACTGCTTAAACTTGAGAGCTAGCATCTTATATGTCAGAAAAGGAATTTCCCCAGTCTTAACAGCGTGATTAACCATAAGGCCGTGTGCAAATGAAATTGCTCTTTCATGTTTTTATGGCATTATTATGTCCTGTTGAATCCTTGATCTTTAGCAGTTTAACCAATCATAAAAGGATCGAATCATGACCTTGTAGATGGTGCGGGTGCTTGTTGCGACAGCAATCAGGGAGGCAGCTGCTGGTGCAGGAGAGGATGCATTAGTAAAGCTGATGGATGCCACATGTCGACGTGCCACTGCACCTCCTGCGCCTCCTGATGGCCTCAGTCTTGTCGATGTAGGTTACTCAGAATTTGATCCACAAAACTGCTTCATTTTAAATGAC

mRNA sequence

ATGATACCAAATCCGAGGGCTAGACTTGTATCAAGTGCCGATTTCGGTTGCAAGTTTCTGATGGAGAATCGAGCTCCGCAAAAGGATTATCTCCATTACAATCACACTGATCGCTGCAGAAATGCTAGATGGACCGCCAGGGAAAGCTACCAACTCATGTATGGAAGGCCGTGGCAAGAAGTGCTTGATTTTTATTTTCATGCTGTTAATGGAAGATTCTCTCCGTCCTCATTGTTTGGAACTGAGACAGTTCATGATCATAATGATGCTGAGATTCCATTGATTTACAACACATCAGAGTTAAATAGAGCTTCAGTTAAAGATAAGACTGGGAGGTGGGCAAGGGCAACATTTAAGATTGTTCTCTCCTACCATGGAAATTCTTTTGATGGATGGCAAAAGCAGCCAGGCTTGAATACTGTTCAAGGTTTGGTAGAAAAATCTCTTGGGAAATTTGTAGATGAGAAGAAAGCTCAGCTGCTGAAAGATAGATGCTTACCACTAGAAGGATGTGCTGTGGTTGCTGGGCGCACTGACAAAGGGGTGACAGCTCTGCGACAAGTTTGCTCATTCTATACTTGGCGCACGGATGTTAAACCTCTAGACATTGAAGATTCGATCAATAGTGCAGCTCCCGGAAAACTCAGAGTCTTGTCTGTTTCTCAGGTGTCTCGTAACTTCCACCCAAATTTCTCTGCTAAATGGAGGCGATATCTGTATATTTTCCCTCTCAAAGATGGAGAGAATATGGAACCAGATTTCGCAATTGGAGAAGACGCAGAAAACTGTAGAGAAGAGGAAAATTATAATAAAATTGAAGCCTTGAATGGTGAATTCAAGGATGAGATTCTGGATAACTTGGTCCTCAGTCAAAATGAGGAGCTTGCATCAGATGATAAGCCTCAAACATTTAGTGTAAGGAGGGTTAACCGACTACTAAGGAAACTAGAAGGAAAGTTATTGTCATACAAGATGTTTGCACGTGACACCAAAGCATCAAGAAATGAAGGTCCACCTACCGAGTGTTTTCTTTACCATGCACGAGCAGTGGAAGCTAGACTACCATGCTTGGATCAAGGAGTAGGAAGAAGTGTTATGTGTGTTGAGCTGGTCGCTAATCGCTTCTTGCGAAAGATGGTGCGGGTGCTTGTTGCGACAGCAATCAGGGAGGCAGCTGCTGGTGCAGGAGAGGATGCATTAGTAAAGCTGATGGATGCCACATGTCGACGTGCCACTGCACCTCCTGCGCCTCCTGATGGCCTCAGTCTTGTCGATGTAGGTTACTCAGAATTTGATCCACAAAACTGCTTCATTTTAAATGAC

Coding sequence (CDS)

ATGATACCAAATCCGAGGGCTAGACTTGTATCAAGTGCCGATTTCGGTTGCAAGTTTCTGATGGAGAATCGAGCTCCGCAAAAGGATTATCTCCATTACAATCACACTGATCGCTGCAGAAATGCTAGATGGACCGCCAGGGAAAGCTACCAACTCATGTATGGAAGGCCGTGGCAAGAAGTGCTTGATTTTTATTTTCATGCTGTTAATGGAAGATTCTCTCCGTCCTCATTGTTTGGAACTGAGACAGTTCATGATCATAATGATGCTGAGATTCCATTGATTTACAACACATCAGAGTTAAATAGAGCTTCAGTTAAAGATAAGACTGGGAGGTGGGCAAGGGCAACATTTAAGATTGTTCTCTCCTACCATGGAAATTCTTTTGATGGATGGCAAAAGCAGCCAGGCTTGAATACTGTTCAAGGTTTGGTAGAAAAATCTCTTGGGAAATTTGTAGATGAGAAGAAAGCTCAGCTGCTGAAAGATAGATGCTTACCACTAGAAGGATGTGCTGTGGTTGCTGGGCGCACTGACAAAGGGGTGACAGCTCTGCGACAAGTTTGCTCATTCTATACTTGGCGCACGGATGTTAAACCTCTAGACATTGAAGATTCGATCAATAGTGCAGCTCCCGGAAAACTCAGAGTCTTGTCTGTTTCTCAGGTGTCTCGTAACTTCCACCCAAATTTCTCTGCTAAATGGAGGCGATATCTGTATATTTTCCCTCTCAAAGATGGAGAGAATATGGAACCAGATTTCGCAATTGGAGAAGACGCAGAAAACTGTAGAGAAGAGGAAAATTATAATAAAATTGAAGCCTTGAATGGTGAATTCAAGGATGAGATTCTGGATAACTTGGTCCTCAGTCAAAATGAGGAGCTTGCATCAGATGATAAGCCTCAAACATTTAGTGTAAGGAGGGTTAACCGACTACTAAGGAAACTAGAAGGAAAGTTATTGTCATACAAGATGTTTGCACGTGACACCAAAGCATCAAGAAATGAAGGTCCACCTACCGAGTGTTTTCTTTACCATGCACGAGCAGTGGAAGCTAGACTACCATGCTTGGATCAAGGAGTAGGAAGAAGTGTTATGTGTGTTGAGCTGGTCGCTAATCGCTTCTTGCGAAAGATGGTGCGGGTGCTTGTTGCGACAGCAATCAGGGAGGCAGCTGCTGGTGCAGGAGAGGATGCATTAGTAAAGCTGATGGATGCCACATGTCGACGTGCCACTGCACCTCCTGCGCCTCCTGATGGCCTCAGTCTTGTCGATGTAGGTTACTCAGAATTTGATCCACAAAACTGCTTCATTTTAAATGAC

Protein sequence

MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQEVLDFYFHAVNGRFSPSSLFGTETVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRTDKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPGKLRVLSVSQVSRNFHPNFSAKWRRYLYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASDDKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALVKLMDATCRRATAPPAPPDGLSLVDVGYSEFDPQNCFILND

Homology

[Close]

BLAST of MS002180 vs. NCBI nr
Match: XP_022135239.1 (uncharacterized protein LOC111007251 isoform X1 [Momordica charantia])

HSP 1 Score: 892.9 bits (2306), Expect = 1.1e-255
Identity = 437/443 (98.65%), Postives = 439/443 (99.10%), Query Frame = 0


Query: 1   MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE 60
           MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE
Sbjct: 1   MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE 60

Query: 61  VLDFYFHAVNGRFSPSSLFGTE--TVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF 120
           VLDFYFHAVNGRFSPSSLFGTE  TVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF
Sbjct: 61  VLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF 120

Query: 121 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT 180
           KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT
Sbjct: 121 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT 180

Query: 181 DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPGKLRVLSVSQVSRNFHPNFSAKWRRY 240
           DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAP KLRVLSVSQVSRNFHPNFSAKWRRY
Sbjct: 181 DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRRY 240

Query: 241 LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD 300
           LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD
Sbjct: 241 LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD 300

Query: 301 DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD 360
           DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD
Sbjct: 301 DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD 360

Query: 361 QGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALVKLMDATCRRATAPPAPP 420
           QGVGRSVMCVELVANRFLRKMVRVLVATAIRE AAGAGEDAL++LMDATCRRATAPPAPP
Sbjct: 361 QGVGRSVMCVELVANRFLRKMVRVLVATAIREVAAGAGEDALLRLMDATCRRATAPPAPP 420

Query: 421 DGLSLVDVGYSEFDPQNCFILND 442
           DGLSLVDVGYSEFDPQNCFILND
Sbjct: 421 DGLSLVDVGYSEFDPQNCFILND 443

[Close]

BLAST of MS002180 vs. NCBI nr
Match: XP_038878348.1 (tRNA pseudouridine synthase A [Benincasa hispida])

HSP 1 Score: 807.7 bits (2085), Expect = 4.8e-230
Identity = 399/445 (89.66%), Postives = 415/445 (93.26%), Query Frame = 0


Query: 1   MIPNPRARLV--SSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 60
           MIPNPRARLV  S A FG K LMENRA QKDYLHYNHTDRCRNARWTARESYQLMYGRPW
Sbjct: 1   MIPNPRARLVSPSGAGFGFKILMENRAQQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 60

Query: 61  QEVLDFYFHAVNGRFSPSSLFGTETVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF 120
           QEVLDFYFHAVNGRFS SSLFGTE  HDH+DAEIPLIYNT+ELNR SVKDKTGRWARATF
Sbjct: 61  QEVLDFYFHAVNGRFSLSSLFGTEIGHDHDDAEIPLIYNTAELNRVSVKDKTGRWARATF 120

Query: 121 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT 180
           KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVD+KKAQ+LKD+CLP+EGCAVVAGRT
Sbjct: 121 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDDKKAQMLKDKCLPIEGCAVVAGRT 180

Query: 181 DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPGKLRVLSVSQVSRNFHPNFSAKWRRY 240
           DKGVTALRQVCSFYTWRTDV+P DIEDSIN AAPGKLR LSVSQVSR FHPNFSAKWRRY
Sbjct: 181 DKGVTALRQVCSFYTWRTDVQPQDIEDSINRAAPGKLRTLSVSQVSRIFHPNFSAKWRRY 240

Query: 241 LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNG-EFKDEILDNLVLSQN-EELA 300
           LYIFPL DGENMEPD+AI ED E+CR+EENY KIE LNG E KDEI DNLV   N EEL 
Sbjct: 241 LYIFPLNDGENMEPDYAIWEDVEDCRKEENYGKIEVLNGEECKDEIHDNLVFGPNDEELV 300

Query: 301 SDDKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPC 360
           S DKPQTFS+RRVN+LLRKLEGKLLSYKMFARDTKASRNEGPPTECF+YHARAVEARLPC
Sbjct: 301 SADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAVEARLPC 360

Query: 361 LDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALVKLMDATCRRATAPPA 420
           LDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDAL++LMDATCRRATAPPA
Sbjct: 361 LDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLELMDATCRRATAPPA 420

Query: 421 PPDGLSLVDVGYSEFDPQNCFILND 442
           PPDGLSLVDVGY+EFD QNCFILND
Sbjct: 421 PPDGLSLVDVGYTEFDSQNCFILND 445

[Close]

BLAST of MS002180 vs. NCBI nr
Match: XP_022135240.1 (uncharacterized protein LOC111007251 isoform X2 [Momordica charantia])

HSP 1 Score: 774.6 bits (1999), Expect = 4.5e-220
Identity = 377/380 (99.21%), Postives = 377/380 (99.21%), Query Frame = 0


Query: 1   MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE 60
           MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE
Sbjct: 1   MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE 60

Query: 61  VLDFYFHAVNGRFSPSSLFGTE--TVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF 120
           VLDFYFHAVNGRFSPSSLFGTE  TVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF
Sbjct: 61  VLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF 120

Query: 121 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT 180
           KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT
Sbjct: 121 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT 180

Query: 181 DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPGKLRVLSVSQVSRNFHPNFSAKWRRY 240
           DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAP KLRVLSVSQVSRNFHPNFSAKWRRY
Sbjct: 181 DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRRY 240

Query: 241 LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD 300
           LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD
Sbjct: 241 LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD 300

Query: 301 DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD 360
           DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD
Sbjct: 301 DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD 360

Query: 361 QGVGRSVMCVELVANRFLRK 379
           QGVGRSVMCVELVANRFLRK
Sbjct: 361 QGVGRSVMCVELVANRFLRK 380

[Close]

BLAST of MS002180 vs. NCBI nr
Match: XP_022933289.1 (uncharacterized protein LOC111440432 isoform X1 [Cucurbita moschata])

HSP 1 Score: 768.8 bits (1984), Expect = 2.5e-218
Identity = 386/446 (86.55%), Postives = 409/446 (91.70%), Query Frame = 0


Query: 1   MIPNPRARLVSSA----DFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGR 60
           MIPNPRA L+SS+     FG K LMENRA +KDYLHYNHTDRCRNARWT+RESYQ MY R
Sbjct: 1   MIPNPRAILLSSSGAGFGFGIKCLMENRAQKKDYLHYNHTDRCRNARWTSRESYQFMYER 60

Query: 61  PWQEVLDFYFHAVNGRFSPSSLFGTETVHDHNDAEIPLIYNTSELNRASVKDKTGRWARA 120
           PWQEVLDFY HAVNGR S SSL GTETV DH+D EI  + NT+ELNRA  KD TGRWARA
Sbjct: 61  PWQEVLDFYSHAVNGRLSLSSL-GTETVRDHDDTEIQPVCNTAELNRAPGKDGTGRWARA 120

Query: 121 TFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAG 180
           TFKIVLSYHGNSFDGWQKQP LNTVQG+VEKSLGKFVDEKKAQ LKDRCLP+EGCAVVAG
Sbjct: 121 TFKIVLSYHGNSFDGWQKQPELNTVQGVVEKSLGKFVDEKKAQQLKDRCLPIEGCAVVAG 180

Query: 181 RTDKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPGKLRVLSVSQVSRNFHPNFSAKWR 240
           RTDKGVTALRQVCSFYTWRTDVKP DIE SIN+AAPGKLRVLSVSQVSR FHPNFSAKWR
Sbjct: 181 RTDKGVTALRQVCSFYTWRTDVKPQDIEGSINNAAPGKLRVLSVSQVSRMFHPNFSAKWR 240

Query: 241 RYLYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQ-NEEL 300
           RYLYIFPLKDGENMEP+FAI ED EN REEE+ +KIEAL+GE KDEIL+NLVLSQ +EE 
Sbjct: 241 RYLYIFPLKDGENMEPNFAIWEDVENIREEEHRDKIEALHGECKDEILNNLVLSQDDEEP 300

Query: 301 ASDDKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLP 360
           AS +KPQTFS+ RVN+LLRKLEGKLLSYKMFARDTKASRNEGPPTECF+YHARA+EARLP
Sbjct: 301 ASANKPQTFSIMRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLP 360

Query: 361 CLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALVKLMDATCRRATAPP 420
           CLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDAL+KLMDATCRRATAPP
Sbjct: 361 CLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPP 420

Query: 421 APPDGLSLVDVGYSEFDPQNCFILND 442
           APPDGLSLVDVGY+EFDPQNCFILND
Sbjct: 421 APPDGLSLVDVGYTEFDPQNCFILND 445

[Close]

BLAST of MS002180 vs. NCBI nr
Match: XP_008453520.1 (PREDICTED: tRNA pseudouridine synthase A [Cucumis melo] >KAA0058161.1 tRNA pseudouridine synthase A [Cucumis melo var. makuwa])

HSP 1 Score: 768.8 bits (1984), Expect = 2.5e-218
Identity = 388/445 (87.19%), Postives = 404/445 (90.79%), Query Frame = 0


Query: 1   MIPNPRARLV--SSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 60
           MIPNPRARLV  SSA FG K LMENRA QKDYLHYNHTDRCRNARWTARES+Q MYGRPW
Sbjct: 1   MIPNPRARLVSPSSAGFGLKILMENRAQQKDYLHYNHTDRCRNARWTARESFQFMYGRPW 60

Query: 61  QEVLDFYFHAVNGRFSPSSLFGTETVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF 120
           QEVLDFY HAVNGR S SSL GTE   DH+DAE PLIYN++ELNRA+VKDKTGRWARA F
Sbjct: 61  QEVLDFYSHAVNGRLSLSSLLGTEICRDHDDAETPLIYNSAELNRAAVKDKTGRWARANF 120

Query: 121 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT 180
           KIVLSYHGNSFDGWQKQPGLNTVQGLVE+SLGKFVDEKKAQ+LKD+CLPLEGCAVVAGRT
Sbjct: 121 KIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDKCLPLEGCAVVAGRT 180

Query: 181 DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPGKLRVLSVSQVSRNFHPNFSAKWRRY 240
           DKGVTALRQVCSFYTWRTDVKP DIEDSINSAAPGKLR LSVSQVSR FHPNFSAKWRRY
Sbjct: 181 DKGVTALRQVCSFYTWRTDVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWRRY 240

Query: 241 LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGE-FKDEILDNLVLSQN-EELA 300
           LYI PL DGENMEPD AI ED EN REEEN  KIEALNGE   D I DNLVLS N +EL 
Sbjct: 241 LYILPLNDGENMEPDSAIWEDVEN-REEENCVKIEALNGEKCVDGIHDNLVLSPNDDELV 300

Query: 301 SDDKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPC 360
           S DKPQTFS+RRVN+LLRKLEGKLLSYKMFARDTKASRNEGPPTECF+YHARA E RLPC
Sbjct: 301 SADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAAETRLPC 360

Query: 361 LDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALVKLMDATCRRATAPPA 420
           L+QGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDAL++LM ATCRRATAPPA
Sbjct: 361 LEQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLELMVATCRRATAPPA 420

Query: 421 PPDGLSLVDVGYSEFDPQNCFILND 442
           PPDGLSLVDVGY+EFD Q CFILND
Sbjct: 421 PPDGLSLVDVGYTEFDSQICFILND 444

[Close]

BLAST of MS002180 vs. ExPASy Swiss-Prot
Match: B5YDX5 (tRNA pseudouridine synthase A OS=Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12) OX=309799 GN=truA PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.8e-14
Identity = 52/134 (38.81%), Postives = 72/134 (53.73%), Query Frame = 0


Query: 118 FKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGR 177
           +K+ LSY G  F G+QKQPG  TVQG +EK L         +LL D  + + G    AGR
Sbjct: 4   WKVELSYIGKDFWGFQKQPGKRTVQGELEKVL---------KLLFDEDIKVIG----AGR 63

Query: 178 TDKGVTALRQVCSFYTWRTDVKPLD-IEDSINSAAPGKLRVLSVSQVSRNFHPNFSAKWR 237
           TD GV AL QV +F T  +     D +   +N   PG +++  V  V  NFH  +SAK R
Sbjct: 64  TDAGVHALGQVVNFKTKESKNFSCDKLYKVLNKLLPGDIKIKKVEIVDDNFHARYSAKRR 123

Query: 238 RYLYIFPLKDGENM 251
            Y+Y+    + +N+
Sbjct: 124 WYIYVIYNNEEKNL 124

[Close]

BLAST of MS002180 vs. ExPASy Swiss-Prot
Match: Q2S4S9 (tRNA pseudouridine synthase A OS=Salinibacter ruber (strain DSM 13855 / M31) OX=309807 GN=truA PE=3 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 3.4e-13
Identity = 87/316 (27.53%), Postives = 121/316 (38.29%), Query Frame = 0


Query: 118 FKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVV-AG 177
           +++++ Y G  F GWQ QP + TVQG +E+++   V                 C V  +G
Sbjct: 4   YRLLVEYDGTDFHGWQIQPTVPTVQGALEEAVDTVVG--------------TACRVTGSG 63

Query: 178 RTDKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPGKLRVLSVSQVSRNFHPNFSAKWR 237
           RTD GV A  QV       T   P  +  S+N   P  + V  V +   +FH  + A+ R
Sbjct: 64  RTDAGVHARGQVAHVDLPETR-DPYRLRGSLNGLTPDAVAVRRVERAPDDFHARYDARRR 123

Query: 238 RYLYIFPLKDGENMEPDFAIGEDAEN-CREEENYNKIEALNGEFKDEILDNLVLSQNEEL 297
           RY Y        + +P  A+G  A    R   ++ +I A   +          L      
Sbjct: 124 RYHYHL------HTQP-CALGRRARTPVRPVPDFGRIRAATPD----------LLGRHNF 183

Query: 298 ASDDKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAV-EARL 357
           +S    Q+ +  RV                                C L H R V EAR 
Sbjct: 184 SSFCLTQSETENRV--------------------------------CTLTHLRWVDEARR 243

Query: 358 PCLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALVKLMDATCRRATAP 417
            C           VE+V  RFL  MVR LV T +         DAL  ++ A  RRA  P
Sbjct: 244 GC---------WRVEVVGTRFLHGMVRALVGTLVEIGHEKRAADALPAVLAARDRRAAGP 246

Query: 418 PAPPDGLSLVDVGYSE 431
             PP+GL L  V Y +
Sbjct: 304 SMPPEGLVLEAVRYEQ 246

[Close]

BLAST of MS002180 vs. ExPASy Swiss-Prot
Match: B5RLM0 (tRNA pseudouridine synthase A OS=Borrelia duttonii (strain Ly) OX=412419 GN=truA PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 4.5e-13
Identity = 45/131 (34.35%), Postives = 73/131 (55.73%), Query Frame = 0


Query: 122 LSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRTDKG 181
           ++Y G+ + G+Q QP   T+QG +EK+L K +++ K ++              AGRTDKG
Sbjct: 8   IAYDGSLYHGFQIQPTKPTIQGEIEKALEK-INKTKVKIQS------------AGRTDKG 67

Query: 182 VTALRQVCSFYTWRTDVKPLDIEDSINSAAPGKLRVLSVSQVSRNFHPNFSAKWRRYLYI 241
           V A RQ+ SFY  + ++   +++ +INS     +R++ +  VS  F P F AK R+Y Y 
Sbjct: 68  VHAKRQIISFYI-KININLKNLKTAINSLLKNDIRIIKLKYVSNEFQPRFHAKKRKYTYY 122

Query: 242 FPLKDGENMEP 253
             + + EN  P
Sbjct: 128 --ILNNENHYP 122

[Close]

BLAST of MS002180 vs. ExPASy Swiss-Prot
Match: B5RQJ5 (tRNA pseudouridine synthase A OS=Borrelia recurrentis (strain A1) OX=412418 GN=truA PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 4.5e-13
Identity = 45/131 (34.35%), Postives = 73/131 (55.73%), Query Frame = 0


Query: 122 LSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRTDKG 181
           ++Y G+ + G+Q QP   T+QG +EK+L K +++ K ++              AGRTDKG
Sbjct: 8   IAYDGSLYHGFQIQPTKPTIQGEIEKALEK-INKTKVKIQS------------AGRTDKG 67

Query: 182 VTALRQVCSFYTWRTDVKPLDIEDSINSAAPGKLRVLSVSQVSRNFHPNFSAKWRRYLYI 241
           V A RQ+ SFY  + ++   +++ +INS     +R++ +  VS  F P F AK R+Y Y 
Sbjct: 68  VHAKRQIISFYI-KININLKNLKTAINSLLKNDIRIIKLKYVSNEFQPRFHAKKRKYTYY 122

Query: 242 FPLKDGENMEP 253
             + + EN  P
Sbjct: 128 --ILNNENHYP 122

[Close]

BLAST of MS002180 vs. ExPASy Swiss-Prot
Match: Q8R601 (tRNA pseudouridine synthase A OS=Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355) OX=190304 GN=truA PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 4.5e-13
Identity = 46/127 (36.22%), Postives = 69/127 (54.33%), Query Frame = 0


Query: 115 RATFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVV 174
           R   KI   Y G+S+ G+Q+QP   TVQG +EK L + V +++  L+             
Sbjct: 5   RKNIKIEFRYDGSSYYGFQRQPNKITVQGEIEKVL-RIVTKEEINLIS------------ 64

Query: 175 AGRTDKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPGKLRVLSVSQVSRNFHPNFSAK 234
           AGRTD+GV A  QV +FYT  +++     +  +  A P  + +LSV +V  NF+   +AK
Sbjct: 65  AGRTDRGVHANHQVSNFYT-SSNIPIEKYKYLLTRALPNDIDILSVEEVDENFNARHNAK 117

Query: 235 WRRYLYI 242
            R Y+YI
Sbjct: 125 MREYIYI 117

[Close]

BLAST of MS002180 vs. ExPASy TrEMBL
Match: A0A6J1C0W1 (tRNA pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111007251 PE=3 SV=1)

HSP 1 Score: 892.9 bits (2306), Expect = 5.5e-256
Identity = 437/443 (98.65%), Postives = 439/443 (99.10%), Query Frame = 0


Query: 1   MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE 60
           MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE
Sbjct: 1   MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE 60

Query: 61  VLDFYFHAVNGRFSPSSLFGTE--TVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF 120
           VLDFYFHAVNGRFSPSSLFGTE  TVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF
Sbjct: 61  VLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF 120

Query: 121 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT 180
           KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT
Sbjct: 121 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT 180

Query: 181 DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPGKLRVLSVSQVSRNFHPNFSAKWRRY 240
           DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAP KLRVLSVSQVSRNFHPNFSAKWRRY
Sbjct: 181 DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRRY 240

Query: 241 LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD 300
           LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD
Sbjct: 241 LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD 300

Query: 301 DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD 360
           DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD
Sbjct: 301 DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD 360

Query: 361 QGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALVKLMDATCRRATAPPAPP 420
           QGVGRSVMCVELVANRFLRKMVRVLVATAIRE AAGAGEDAL++LMDATCRRATAPPAPP
Sbjct: 361 QGVGRSVMCVELVANRFLRKMVRVLVATAIREVAAGAGEDALLRLMDATCRRATAPPAPP 420

Query: 421 DGLSLVDVGYSEFDPQNCFILND 442
           DGLSLVDVGYSEFDPQNCFILND
Sbjct: 421 DGLSLVDVGYSEFDPQNCFILND 443

[Close]

BLAST of MS002180 vs. ExPASy TrEMBL
Match: A0A6J1C0L5 (uncharacterized protein LOC111007251 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007251 PE=4 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 2.2e-220
Identity = 377/380 (99.21%), Postives = 377/380 (99.21%), Query Frame = 0


Query: 1   MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE 60
           MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE
Sbjct: 1   MIPNPRARLVSSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPWQE 60

Query: 61  VLDFYFHAVNGRFSPSSLFGTE--TVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF 120
           VLDFYFHAVNGRFSPSSLFGTE  TVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF
Sbjct: 61  VLDFYFHAVNGRFSPSSLFGTEVKTVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF 120

Query: 121 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT 180
           KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT
Sbjct: 121 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT 180

Query: 181 DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPGKLRVLSVSQVSRNFHPNFSAKWRRY 240
           DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAP KLRVLSVSQVSRNFHPNFSAKWRRY
Sbjct: 181 DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPRKLRVLSVSQVSRNFHPNFSAKWRRY 240

Query: 241 LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD 300
           LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD
Sbjct: 241 LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQNEELASD 300

Query: 301 DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD 360
           DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD
Sbjct: 301 DKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLD 360

Query: 361 QGVGRSVMCVELVANRFLRK 379
           QGVGRSVMCVELVANRFLRK
Sbjct: 361 QGVGRSVMCVELVANRFLRK 380

[Close]

BLAST of MS002180 vs. ExPASy TrEMBL
Match: A0A5A7UXE7 (tRNA pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G004790 PE=3 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 1.2e-218
Identity = 388/445 (87.19%), Postives = 404/445 (90.79%), Query Frame = 0


Query: 1   MIPNPRARLV--SSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 60
           MIPNPRARLV  SSA FG K LMENRA QKDYLHYNHTDRCRNARWTARES+Q MYGRPW
Sbjct: 1   MIPNPRARLVSPSSAGFGLKILMENRAQQKDYLHYNHTDRCRNARWTARESFQFMYGRPW 60

Query: 61  QEVLDFYFHAVNGRFSPSSLFGTETVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF 120
           QEVLDFY HAVNGR S SSL GTE   DH+DAE PLIYN++ELNRA+VKDKTGRWARA F
Sbjct: 61  QEVLDFYSHAVNGRLSLSSLLGTEICRDHDDAETPLIYNSAELNRAAVKDKTGRWARANF 120

Query: 121 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT 180
           KIVLSYHGNSFDGWQKQPGLNTVQGLVE+SLGKFVDEKKAQ+LKD+CLPLEGCAVVAGRT
Sbjct: 121 KIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDKCLPLEGCAVVAGRT 180

Query: 181 DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPGKLRVLSVSQVSRNFHPNFSAKWRRY 240
           DKGVTALRQVCSFYTWRTDVKP DIEDSINSAAPGKLR LSVSQVSR FHPNFSAKWRRY
Sbjct: 181 DKGVTALRQVCSFYTWRTDVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWRRY 240

Query: 241 LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGE-FKDEILDNLVLSQN-EELA 300
           LYI PL DGENMEPD AI ED EN REEEN  KIEALNGE   D I DNLVLS N +EL 
Sbjct: 241 LYILPLNDGENMEPDSAIWEDVEN-REEENCVKIEALNGEKCVDGIHDNLVLSPNDDELV 300

Query: 301 SDDKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPC 360
           S DKPQTFS+RRVN+LLRKLEGKLLSYKMFARDTKASRNEGPPTECF+YHARA E RLPC
Sbjct: 301 SADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAAETRLPC 360

Query: 361 LDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALVKLMDATCRRATAPPA 420
           L+QGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDAL++LM ATCRRATAPPA
Sbjct: 361 LEQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLELMVATCRRATAPPA 420

Query: 421 PPDGLSLVDVGYSEFDPQNCFILND 442
           PPDGLSLVDVGY+EFD Q CFILND
Sbjct: 421 PPDGLSLVDVGYTEFDSQICFILND 444

[Close]

BLAST of MS002180 vs. ExPASy TrEMBL
Match: A0A6J1EZC2 (tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111440432 PE=3 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 1.2e-218
Identity = 386/446 (86.55%), Postives = 409/446 (91.70%), Query Frame = 0


Query: 1   MIPNPRARLVSSA----DFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGR 60
           MIPNPRA L+SS+     FG K LMENRA +KDYLHYNHTDRCRNARWT+RESYQ MY R
Sbjct: 1   MIPNPRAILLSSSGAGFGFGIKCLMENRAQKKDYLHYNHTDRCRNARWTSRESYQFMYER 60

Query: 61  PWQEVLDFYFHAVNGRFSPSSLFGTETVHDHNDAEIPLIYNTSELNRASVKDKTGRWARA 120
           PWQEVLDFY HAVNGR S SSL GTETV DH+D EI  + NT+ELNRA  KD TGRWARA
Sbjct: 61  PWQEVLDFYSHAVNGRLSLSSL-GTETVRDHDDTEIQPVCNTAELNRAPGKDGTGRWARA 120

Query: 121 TFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAG 180
           TFKIVLSYHGNSFDGWQKQP LNTVQG+VEKSLGKFVDEKKAQ LKDRCLP+EGCAVVAG
Sbjct: 121 TFKIVLSYHGNSFDGWQKQPELNTVQGVVEKSLGKFVDEKKAQQLKDRCLPIEGCAVVAG 180

Query: 181 RTDKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPGKLRVLSVSQVSRNFHPNFSAKWR 240
           RTDKGVTALRQVCSFYTWRTDVKP DIE SIN+AAPGKLRVLSVSQVSR FHPNFSAKWR
Sbjct: 181 RTDKGVTALRQVCSFYTWRTDVKPQDIEGSINNAAPGKLRVLSVSQVSRMFHPNFSAKWR 240

Query: 241 RYLYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGEFKDEILDNLVLSQ-NEEL 300
           RYLYIFPLKDGENMEP+FAI ED EN REEE+ +KIEAL+GE KDEIL+NLVLSQ +EE 
Sbjct: 241 RYLYIFPLKDGENMEPNFAIWEDVENIREEEHRDKIEALHGECKDEILNNLVLSQDDEEP 300

Query: 301 ASDDKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLP 360
           AS +KPQTFS+ RVN+LLRKLEGKLLSYKMFARDTKASRNEGPPTECF+YHARA+EARLP
Sbjct: 301 ASANKPQTFSIMRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAMEARLP 360

Query: 361 CLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALVKLMDATCRRATAPP 420
           CLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDAL+KLMDATCRRATAPP
Sbjct: 361 CLDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLKLMDATCRRATAPP 420

Query: 421 APPDGLSLVDVGYSEFDPQNCFILND 442
           APPDGLSLVDVGY+EFDPQNCFILND
Sbjct: 421 APPDGLSLVDVGYTEFDPQNCFILND 445

[Close]

BLAST of MS002180 vs. ExPASy TrEMBL
Match: A0A1S3BWI6 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103494203 PE=3 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 1.2e-218
Identity = 388/445 (87.19%), Postives = 404/445 (90.79%), Query Frame = 0


Query: 1   MIPNPRARLV--SSADFGCKFLMENRAPQKDYLHYNHTDRCRNARWTARESYQLMYGRPW 60
           MIPNPRARLV  SSA FG K LMENRA QKDYLHYNHTDRCRNARWTARES+Q MYGRPW
Sbjct: 1   MIPNPRARLVSPSSAGFGLKILMENRAQQKDYLHYNHTDRCRNARWTARESFQFMYGRPW 60

Query: 61  QEVLDFYFHAVNGRFSPSSLFGTETVHDHNDAEIPLIYNTSELNRASVKDKTGRWARATF 120
           QEVLDFY HAVNGR S SSL GTE   DH+DAE PLIYN++ELNRA+VKDKTGRWARA F
Sbjct: 61  QEVLDFYSHAVNGRLSLSSLLGTEICRDHDDAETPLIYNSAELNRAAVKDKTGRWARANF 120

Query: 121 KIVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRT 180
           KIVLSYHGNSFDGWQKQPGLNTVQGLVE+SLGKFVDEKKAQ+LKD+CLPLEGCAVVAGRT
Sbjct: 121 KIVLSYHGNSFDGWQKQPGLNTVQGLVERSLGKFVDEKKAQMLKDKCLPLEGCAVVAGRT 180

Query: 181 DKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPGKLRVLSVSQVSRNFHPNFSAKWRRY 240
           DKGVTALRQVCSFYTWRTDVKP DIEDSINSAAPGKLR LSVSQVSR FHPNFSAKWRRY
Sbjct: 181 DKGVTALRQVCSFYTWRTDVKPQDIEDSINSAAPGKLRTLSVSQVSRIFHPNFSAKWRRY 240

Query: 241 LYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEALNGE-FKDEILDNLVLSQN-EELA 300
           LYI PL DGENMEPD AI ED EN REEEN  KIEALNGE   D I DNLVLS N +EL 
Sbjct: 241 LYILPLNDGENMEPDSAIWEDVEN-REEENCVKIEALNGEKCVDGIHDNLVLSPNDDELV 300

Query: 301 SDDKPQTFSVRRVNRLLRKLEGKLLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPC 360
           S DKPQTFS+RRVN+LLRKLEGKLLSYKMFARDTKASRNEGPPTECF+YHARA E RLPC
Sbjct: 301 SADKPQTFSIRRVNQLLRKLEGKLLSYKMFARDTKASRNEGPPTECFVYHARAAETRLPC 360

Query: 361 LDQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALVKLMDATCRRATAPPA 420
           L+QGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDAL++LM ATCRRATAPPA
Sbjct: 361 LEQGVGRSVMCVELVANRFLRKMVRVLVATAIREAAAGAGEDALLELMVATCRRATAPPA 420

Query: 421 PPDGLSLVDVGYSEFDPQNCFILND 442
           PPDGLSLVDVGY+EFD Q CFILND
Sbjct: 421 PPDGLSLVDVGYTEFDSQICFILND 444

[Close]

BLAST of MS002180 vs. TAIR 10
Match: AT5G35400.1 (Pseudouridine synthase family protein )

HSP 1 Score: 503.1 bits (1294), Expect = 2.3e-142
Identity = 257/419 (61.34%), Postives = 316/419 (75.42%), Query Frame = 0


Query: 30  YLHYNHTDRCRNARWTARESYQLMYGRPWQEVLDFYFHAVNGRFSPSSLFGTETVHDHND 89
           Y+HY+H D C  ARW ARE+++ MY RPWQ VLDFY +AV G  S  SLF  +    H+D
Sbjct: 6   YVHYHHADACSKARWNARETFRFMYDRPWQHVLDFYSNAVEGNLSVLSLFQPKKSLVHDD 65

Query: 90  AEIPLIYNTSELNRASVKD-KTGRWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKS 149
                +   SE   ++ KD ++GRW R  FKIVLSY+G SFDGWQKQP L+TVQG+VEKS
Sbjct: 66  GITEEMLLESEPETSTRKDGRSGRWERVNFKIVLSYNGASFDGWQKQPDLHTVQGVVEKS 125

Query: 150 LGKFVDEKKAQLLKDRCLPLEGCAVVAGRTDKGVTALRQVCSFYTWRTDVKPLDIEDSIN 209
           LG FVDE+KAQLLK +C PLEG  +VAGRTDKGV+AL QVCSFYTWR D++P+ IED+IN
Sbjct: 126 LGGFVDERKAQLLKKKCKPLEGRVLVAGRTDKGVSALNQVCSFYTWRKDIEPIAIEDAIN 185

Query: 210 SAAPGKLRVLSVSQVSRNFHPNFSAKWRRYLYIFPLKDGENMEPDFAIGEDAENCREEEN 269
             A GKLRV+S+S+VSR+FHPNFSAKWRRYLYIFPL D E +      GE+ EN   +E 
Sbjct: 186 KDASGKLRVVSISKVSRSFHPNFSAKWRRYLYIFPLDDAEPLRKS---GENHENFIFDE- 245

Query: 270 YNKIEALNGEFKDEILDNLVLSQNEELASD---------DKPQTFSVRRVNRLLRKLEGK 329
            NK +  NG   +EI + +++S+++EL  +         +KP  FSV +V++LL+ L+GK
Sbjct: 246 -NKKKQRNGLLSEEIAEEVIMSEDDELEIEETSNALEVVEKPSDFSVSKVDQLLQHLQGK 305

Query: 330 LLSYKMFARDTKASRNEGPPTECFLYHARAVEARLPCLDQGVGRSVMCVELVANRFLRKM 389
           +LSYKMFARDTKA+RNEGPPTECF+YHARA E RL   D   GR VMCVELVANRFLRKM
Sbjct: 306 VLSYKMFARDTKAARNEGPPTECFMYHARAAEIRLSSSDCVEGRRVMCVELVANRFLRKM 365

Query: 390 VRVLVATAIREAAAGAGEDALVKLMDATCRRATAPPAPPDGLSLVDVGYSEFDPQNCFI 439
           VRVLVAT+IREAAAGA +DAL+KL++A+CRRATAPPAP +GL L DVGY++FDPQ   I
Sbjct: 366 VRVLVATSIREAAAGAEDDALLKLLEASCRRATAPPAPSEGLCLFDVGYTDFDPQTSLI 419

[Close]

BLAST of MS002180 vs. TAIR 10
Match: AT5G35400.2 (Pseudouridine synthase family protein )

HSP 1 Score: 479.6 bits (1233), Expect = 2.8e-135
Identity = 257/470 (54.68%), Postives = 316/470 (67.23%), Query Frame = 0


Query: 30  YLHYNHTDRCRNARWTARESYQLMYGRPWQEVLDFYFHAVNGRFSPSSLFGTETVHDHND 89
           Y+HY+H D C  ARW ARE+++ MY RPWQ VLDFY +AV G  S  SLF  +    H+D
Sbjct: 6   YVHYHHADACSKARWNARETFRFMYDRPWQHVLDFYSNAVEGNLSVLSLFQPKKSLVHDD 65

Query: 90  AEIPLIYNTSELNRASVKD-KTGRWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKS 149
                +   SE   ++ KD ++GRW R  FKIVLSY+G SFDGWQKQP L+TVQG+VEKS
Sbjct: 66  GITEEMLLESEPETSTRKDGRSGRWERVNFKIVLSYNGASFDGWQKQPDLHTVQGVVEKS 125

Query: 150 LGKFVDEKKAQLLKDRCLPLEGCAVVAGRTDKGVTALRQVCSFYTWRTDVKPLDIEDSIN 209
           LG FVDE+KAQLLK +C PLEG  +VAGRTDKGV+AL QVCSFYTWR D++P+ IED+IN
Sbjct: 126 LGGFVDERKAQLLKKKCKPLEGRVLVAGRTDKGVSALNQVCSFYTWRKDIEPIAIEDAIN 185

Query: 210 SAAPGKLRVLSVSQVSRNFHPNFSAKWRRYLYIFPLKDGENMEPDFAIGEDAENCREEEN 269
             A GKLRV+S+S+VSR+FHPNFSAKWRRYLYIFPL D E +      GE+ EN   +E 
Sbjct: 186 KDASGKLRVVSISKVSRSFHPNFSAKWRRYLYIFPLDDAEPLRKS---GENHENFIFDE- 245

Query: 270 YNKIEALNGEFKDEILDNLVLSQNEELASD---------DKPQTFSVRRVNRLLRKLEGK 329
            NK +  NG   +EI + +++S+++EL  +         +KP  FSV +V++LL+ L+GK
Sbjct: 246 -NKKKQRNGLLSEEIAEEVIMSEDDELEIEETSNALEVVEKPSDFSVSKVDQLLQHLQGK 305

Query: 330 LLSYKMFARDTKASRNEGPPTECFLYHARAVEARLP------------------------ 389
           +LSYKMFARDTKA+RNEGPPTECF+YHARA E RL                         
Sbjct: 306 VLSYKMFARDTKAARNEGPPTECFMYHARAAEIRLSSSVVHFVAWVFGGSIVLVILATEG 365

Query: 390 ---------------------------CLDQGVGRSVMCVELVANRFLRKMVRVLVATAI 439
                                        D   GR VMCVELVANRFLRKMVRVLVAT+I
Sbjct: 366 STLDLTDTKLYIASWFLIKRSCEFLFIASDCVEGRRVMCVELVANRFLRKMVRVLVATSI 425

[Close]

BLAST of MS002180 vs. TAIR 10
Match: AT1G20370.1 (Pseudouridine synthase family protein )

HSP 1 Score: 63.9 bits (154), Expect = 3.6e-10
Identity = 48/167 (28.74%), Postives = 77/167 (46.11%), Query Frame = 0


Query: 112 RWARATFKIVLSYHGNSFDGWQKQPGLNTVQGLVEKSL---GKFVDEKKAQLLKDRCLPL 171
           ++ R    IV ++ G  + G QK PG  T++G +E++L   G   D        DR  P 
Sbjct: 54  KYRRRKVAIVFAFCGVGYQGMQKNPGAKTIEGELEEALFHAGAVPD-------ADRNKPR 113

Query: 172 EGCAVVAGRTDKGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPGKLRVLSVSQVSRNFH 231
                 + RTDKGV+A+ QV S    R  V P    + +NS  P ++RV    +V+ +F 
Sbjct: 114 NYEWARSARTDKGVSAVGQVVS---GRFYVDPPGFVERLNSKLPDQIRVFGYKRVAPSFS 173

Query: 232 PNFSAKWRRYLYIFPLKDGENMEPDFAIGEDAENCREEENYNKIEAL 276
                  RRY+Y+ P+        D  +  +AE  R +  Y  ++ +
Sbjct: 174 SKKFCDRRRYVYLIPV-----FALDPCVHGEAEMVRTDLGYEYVKCV 205

[Close]

BLAST of MS002180 vs. TAIR 10
Match: AT1G76120.1 (Pseudouridine synthase family protein )

HSP 1 Score: 63.2 bits (152), Expect = 6.2e-10
Identity = 56/209 (26.79%), Postives = 92/209 (44.02%), Query Frame = 0


Query: 120 IVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRTD 179
           I+ ++ G  + G QK PG  T++G +E++L       ++   K +       A    RTD
Sbjct: 26  IIFAFCGVGYQGMQKNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSA----RTD 85

Query: 180 KGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPGKLRVLSVSQVSRNFHPNFSAKWRRYL 239
           KGV+A+ QV S    R  V PL   + +NS  P ++R+     V+ +F        RRY+
Sbjct: 86  KGVSAVGQVVS---GRFIVDPLGFVNRLNSNLPNQIRIFGYKHVTPSFSSKKFCDRRRYV 145

Query: 240 YIFPL--------KDGENMEPDFAIGEDAENCRE-EENYNKI-EALNGEFKDEILDNLVL 299
           Y+ P+        +D E +      GE+   C E  E   KI   L G++K        L
Sbjct: 146 YLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSERGRKIPPGLVGKWKGTNFGTKSL 205

Query: 300 SQNEELASDDKPQTFSVRRVNRLLRKLEG 319
               +++S++     S  ++  L   L G
Sbjct: 206 DFQSDISSNNSSALRSDIKIEALSSNLAG 227

[Close]

BLAST of MS002180 vs. TAIR 10
Match: AT1G76120.2 (Pseudouridine synthase family protein )

HSP 1 Score: 63.2 bits (152), Expect = 6.2e-10
Identity = 56/209 (26.79%), Postives = 92/209 (44.02%), Query Frame = 0


Query: 120 IVLSYHGNSFDGWQKQPGLNTVQGLVEKSLGKFVDEKKAQLLKDRCLPLEGCAVVAGRTD 179
           I+ ++ G  + G QK PG  T++G +E++L       ++   K +       A    RTD
Sbjct: 26  IIFAFCGVGYQGMQKNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSA----RTD 85

Query: 180 KGVTALRQVCSFYTWRTDVKPLDIEDSINSAAPGKLRVLSVSQVSRNFHPNFSAKWRRYL 239
           KGV+A+ QV S    R  V PL   + +NS  P ++R+     V+ +F        RRY+
Sbjct: 86  KGVSAVGQVVS---GRFIVDPLGFVNRLNSNLPNQIRIFGYKHVTPSFSSKKFCDRRRYV 145

Query: 240 YIFPL--------KDGENMEPDFAIGEDAENCRE-EENYNKI-EALNGEFKDEILDNLVL 299
           Y+ P+        +D E +      GE+   C E  E   KI   L G++K        L
Sbjct: 146 YLLPVFALDPISHRDRETVMASLGPGEEYVKCFECSERGRKIPPGLVGKWKGTNFGTKSL 205

Query: 300 SQNEELASDDKPQTFSVRRVNRLLRKLEG 319
               +++S++     S  ++  L   L G
Sbjct: 206 DFQSDISSNNSSALRSDIKIEALSSNLAG 227

The following BLAST results are available for this feature:

BLAST of MS002180 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135239.1	1.1e-255	98.65	uncharacterized protein LOC111007251 isoform X1 [Momordica charantia]	[more]
XP_038878348.1	4.8e-230	89.66	tRNA pseudouridine synthase A [Benincasa hispida]	[more]
XP_022135240.1	4.5e-220	99.21	uncharacterized protein LOC111007251 isoform X2 [Momordica charantia]	[more]
XP_022933289.1	2.5e-218	86.55	uncharacterized protein LOC111440432 isoform X1 [Cucurbita moschata]	[more]
XP_008453520.1	2.5e-218	87.19	PREDICTED: tRNA pseudouridine synthase A [Cucumis melo] >KAA0058161.1 tRNA pseud...	[more]

BLAST of MS002180 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5YDX5	1.8e-14	38.81	tRNA pseudouridine synthase A OS=Dictyoglomus thermophilum (strain ATCC 35947 / ...	[more]
Q2S4S9	3.4e-13	27.53	tRNA pseudouridine synthase A OS=Salinibacter ruber (strain DSM 13855 / M31) OX=...	[more]
B5RLM0	4.5e-13	34.35	tRNA pseudouridine synthase A OS=Borrelia duttonii (strain Ly) OX=412419 GN=truA...	[more]
B5RQJ5	4.5e-13	34.35	tRNA pseudouridine synthase A OS=Borrelia recurrentis (strain A1) OX=412418 GN=t...	[more]
Q8R601	4.5e-13	36.22	tRNA pseudouridine synthase A OS=Fusobacterium nucleatum subsp. nucleatum (strai...	[more]

BLAST of MS002180 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C0W1	5.5e-256	98.65	tRNA pseudouridine synthase OS=Momordica charantia OX=3673 GN=LOC111007251 PE=3 ...	[more]
A0A6J1C0L5	2.2e-220	99.21	uncharacterized protein LOC111007251 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A5A7UXE7	1.2e-218	87.19	tRNA pseudouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf...	[more]
A0A6J1EZC2	1.2e-218	86.55	tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111440432 PE=3 S...	[more]
A0A1S3BWI6	1.2e-218	87.19	tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103494203 PE=3 SV=1	[more]

BLAST of MS002180 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35400.1	2.3e-142	61.34	Pseudouridine synthase family protein	[more]
AT5G35400.2	2.8e-135	54.68	Pseudouridine synthase family protein	[more]
AT1G20370.1	3.6e-10	28.74	Pseudouridine synthase family protein	[more]
AT1G76120.1	6.2e-10	26.79	Pseudouridine synthase family protein	[more]
AT1G76120.2	6.2e-10	26.79	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 282..426 e-value: 4.4E-14 score: 54.4
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 338..428 e-value: 7.3E-7 score: 29.6
IPR020094	Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal	GENE3D	3.30.70.580		coord: 115..233 e-value: 7.3E-25 score: 89.3
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 38..438
None	No IPR available	PANTHER	PTHR11142:SF10	TRNA PSEUDOURIDINE SYNTHASE	coord: 38..438
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 117..433

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS002180.1	MS002180.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding