MS002161 (gene) Bitter gourd (TR) v1

Overview
NameMS002161
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionMicrosomal prostaglandin E synthase 2
Locationscaffold30: 2866432 .. 2870229 (-)
RNA-Seq ExpressionMS002161
SyntenyMS002161
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAAGATCTTCCTCCATCGCTTCATCCTTCCTCTCCCGCTCAATCTGCACCGCTCATGGCAGCCTTTCCGCCACCGCCACCTCAAATCAAGCTGCGCTGTTCAGTACCACCACCACCGCCGATTCCTATGCCCATCGCCGCTGGTTCTCCTCCCTCCTGGATTCCGTCGCCGGTGGTTCCGCCCGAGCTGCGTCCTTCGGGGTCGTTGGAGCCGTTGCGTCCATAACAGCTGCCTTGTCTATGTCTCAGGAGGTTTATGCGAAGGAGCCCTTGCAGCAGGAGCTGGTTCCTAAGGAGGTCGTTCTTTATCAATACGAAGCGTGTCCGTTTTGTAATAAAGTTAAAGGTAAACTTCTTAATTTGTCGTTCTCGTTGATCTCAATCTTCTGGAATTTTGGATTGGTAATTGCGTCGGTTTACTTGTATGGCTATGACTCGATCAATTTTGTGTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAAGGTGGTGGATCTCGATTGAGGGTCTTTGGGAGAGCGGAAATGTATAGTGGAATCAAGGCAACAGATTTTACCGTTTTCAGGATAACCAATTTTAATACTCTTTCTGTTCCCACCTTACGAGGGTGAGCCATTCCTGCTGCCGGATTGCCTTCTTCTTTTGGCTACTATATGAATAGATTTTAGGATTACTTTGGGTAAGGAGAGCTGAATTATCCATGAAGACTATGGCTAGTGTTTGTATCTGCTGCTGAAATACAGTGAAGTCTCGGACTTTTTAAATCCACTTATTTTCATAAACATGATAAGACTGTTAAAAGTCAAGCGAATTGTATGTGCTTTCCACTATATTTTGGTTTAGTCATTTTACCAAAAATTGCAAATTCACTATGGCCATAATTGTGCTTTTCAAATCTCTCCATGCGGTTACATGCTCTTGTGCACTTAATGAGGACGGTGTCTTACTTTCAATCTCAGCTTTTCTCGACTACTACGATATACCTTATAAAGTTGTTGAGGTCAATCCATTTAGCAAGAAAGAGATTAAATGGTCAGATTATAAGAAGGTGCCAATATTGGTGGTTGATGGTGAACAGCTGGTTGACTCGTCAGGTATCATTTCATTATTCATTATTCTGAATGTTCTTTTGTATATATGAAATAGCACATCTCAGTTTATTCAAGCGAATTATTTTACTACCACATTCAATTTTCATTTTATGTTTAGACTAACTTCTGACAATGGCATTACGCAGCCATTATTGACCAGTTGAGCCACAAGGTTCTGTTGGACAGAAATACTGATTCGGTCTCCGAAGATGATGAAGAGACAAAATGGCGTCGGTAGGTTTCTATTTAGAGGTTATCTTTTTCCAAGTTTAGGTTTCTTAAATTATTTGAATAAAGAATACTCTCCCTTTTCCATACAACTGAATCACATGTAAATTTAGTGGCAGGGGAGGGGAAAGGGAGATTAATGATCGTTTATCGGGTCCAACCACTTATGTCTCAATGATCATATACTTGATTATAGATACACCAAGTGAAAAAGTTTTATGTTATTTAAAGTGTTAAGATTCTGAAAAATTATGATAACAGAGTAAAGGCTACGTATATTATGGGTCTTCTCGTGCTATTAGGCACAGTTTCGTCTGGAAGCTTAAGCTTAAACCAGATGCATGTTGACTTTTTATGAGTCGACGTTGCATTCTTTCCTTGTACTATGCAAGTCCATTTTTGATAATAGTTCCTTTATTGTAGCATAGTTATTTTGTCTTCTTCTTATTCTTTTAGTTGATAATTGAATTTCCAGTCATTAAAGTATGAAACTTCTTGCTTGTGATGCTTTTCATTTGCTTAACCTTTTATACTTCATGGTTCTTGATGCAGATGGGTAGATAATCACTTGGTACATGTCTTATCACCAAACATATATCGAAATACTTCCGAGGCCCTTGAATCATTTGACTACATTGCTAGTAATGGTATGACCGAATCATGGATATATTTACTGAAATGTTCCACTTAACAAACGTATCTTAACGTGGATTAAGGATTTTTCATTGGCATTCTTTCCTGTTAAAGTCTATTTCAGTTAATTGTTACTTTACACCTTAAGTTTTTCCATGTGATGCAAGGGAAATTTAGAAAGTCAAACTTCATAGATAAATTTTGGAATTTTCGTTTATTTGGATTGCTATTTATATTGTAGCTAGTTTCTAATATGTTGGGTGATAAATGGTTTGAGGATTCAGTTAGGATTTGGATTCTCATTCTTATTATAACTTTATTTTGATTGAGACTCTCGTTAATATCACCTAGTGTATATATTCGGTTTTGGATTGAGATTGTGGGTGTGTTTGGGAGTGATTCACTTTTGTCTTTGACATTGTCCAAATCAAATTTTTTTTCCATTGATCATGTTTTTGGCAAATTAAAAATTGATTTTAAGAAAGCTTAAATTCACTTTAAATTGATTTTTAAGGTATCACTTGGGTGGTGATTTTAGAAAATAAATTATAGTTAAATCTCTAATTTTACCATTACTCCTGTATTAACCGAGGGGTGATTACTGGATTTGTTATAATGTATTTCATTTGTCCGCTTCTCTTGCCAATCCTATTTTTTAGGTAACTTTGGTGCTGCTGAAAAGATTACTGTAAAGTATGCTGGGGCAGCTGCTATGTATTTCGTGTCAAAGAAACTGAAGAAGAAATATAATATTACTGATGAGCGTGCAGCCTTATATGAAGCAGCAGAAACGTGGGTAGATGCTTTGAATGGACGGGACTTCCTAGGTATGTTCTTTGAAGTGTCTTTTATATGCACATACAATATAACATGAGCAGCATAAACACGATCTCTTGTTGTTTAGATGATGCGTCTGTACATGATATGAATTGTAACACCGACTTAAAAAAAAAGAGCTTTTAATATTGGTGTTAATGCAGTCCTAAAACTCTGCCTTCAACATGCTATATATGTATATCTATATATAATATATAAGCAACAAGGTAGTGACTAGTGTTGCACTAATCAAGTGTAAAAACAAAAGGCTGGAGAAATTTTAATTGGTTAAAGAAACAATTTCATTGATGATATGAAATTATAACAAAGAAATTAAAAGGAAGTACCATCCCTTGTTTGCCCCTGGCAATTTTCCCACCGAAAATTGCAAACTGAGTTGTTATGGAACTTTTTTCATAAAAAAAGAAGACAGGGAAAACGAAGTTAGGAATGAGATTCAAGGATTGAATATATAGTTGATCTTTTAGAATCAATTTTCCTTCACAACGACACTTAAAGATATGCCAAATTCACATCCTTCTGTATGCCAAAGTTCAGTCAATATTTTTCTCTAGTGACATGAAATTCAATGGTTCTTGTTTTGCGTTGTTTAGGAGGCTCAAAACCTAATCTAGCTGATCTTGCGGTGTTCGGGGTGTTAAGACCCATCCGTTACCTGAGATCTGGTAAAGACATGGTGGAGCACACACGTATTGGTGAATGGTACGCCAAAATGGAGAGCATTGTGGGAGACTCCTCCAGGATCAAAGCC

mRNA sequence

ATGAGAAGATCTTCCTCCATCGCTTCATCCTTCCTCTCCCGCTCAATCTGCACCGCTCATGGCAGCCTTTCCGCCACCGCCACCTCAAATCAAGCTGCGCTGTTCAGTACCACCACCACCGCCGATTCCTATGCCCATCGCCGCTGGTTCTCCTCCCTCCTGGATTCCGTCGCCGGTGGTTCCGCCCGAGCTGCGTCCTTCGGGGTCGTTGGAGCCGTTGCGTCCATAACAGCTGCCTTGTCTATGTCTCAGGAGGTTTATGCGAAGGAGCCCTTGCAGCAGGAGCTGGTTCCTAAGGAGGTCGTTCTTTATCAATACGAAGCGTGTCCGTTTTGTAATAAAGTTAAAGCTTTTCTCGACTACTACGATATACCTTATAAAGTTGTTGAGGTCAATCCATTTAGCAAGAAAGAGATTAAATGGTCAGATTATAAGAAGGTGCCAATATTGGTGGTTGATGGTGAACAGCTGGTTGACTCGTCAGCCATTATTGACCAGTTGAGCCACAAGGTTCTGTTGGACAGAAATACTGATTCGGTCTCCGAAGATGATGAAGAGACAAAATGGCGTCGATGGGTAGATAATCACTTGGTACATGTCTTATCACCAAACATATATCGAAATACTTCCGAGGCCCTTGAATCATTTGACTACATTGCTAGTAATGGTAACTTTGGTGCTGCTGAAAAGATTACTGTAAAGTATGCTGGGGCAGCTGCTATGTATTTCGTGTCAAAGAAACTGAAGAAGAAATATAATATTACTGATGAGCGTGCAGCCTTATATGAAGCAGCAGAAACGTGGGTAGATGCTTTGAATGGACGGGACTTCCTAGGAGGCTCAAAACCTAATCTAGCTGATCTTGCGGTGTTCGGGGTGTTAAGACCCATCCGTTACCTGAGATCTGGTAAAGACATGGTGGAGCACACACGTATTGGTGAATGGTACGCCAAAATGGAGAGCATTGTGGGAGACTCCTCCAGGATCAAAGCC

Coding sequence (CDS)

ATGAGAAGATCTTCCTCCATCGCTTCATCCTTCCTCTCCCGCTCAATCTGCACCGCTCATGGCAGCCTTTCCGCCACCGCCACCTCAAATCAAGCTGCGCTGTTCAGTACCACCACCACCGCCGATTCCTATGCCCATCGCCGCTGGTTCTCCTCCCTCCTGGATTCCGTCGCCGGTGGTTCCGCCCGAGCTGCGTCCTTCGGGGTCGTTGGAGCCGTTGCGTCCATAACAGCTGCCTTGTCTATGTCTCAGGAGGTTTATGCGAAGGAGCCCTTGCAGCAGGAGCTGGTTCCTAAGGAGGTCGTTCTTTATCAATACGAAGCGTGTCCGTTTTGTAATAAAGTTAAAGCTTTTCTCGACTACTACGATATACCTTATAAAGTTGTTGAGGTCAATCCATTTAGCAAGAAAGAGATTAAATGGTCAGATTATAAGAAGGTGCCAATATTGGTGGTTGATGGTGAACAGCTGGTTGACTCGTCAGCCATTATTGACCAGTTGAGCCACAAGGTTCTGTTGGACAGAAATACTGATTCGGTCTCCGAAGATGATGAAGAGACAAAATGGCGTCGATGGGTAGATAATCACTTGGTACATGTCTTATCACCAAACATATATCGAAATACTTCCGAGGCCCTTGAATCATTTGACTACATTGCTAGTAATGGTAACTTTGGTGCTGCTGAAAAGATTACTGTAAAGTATGCTGGGGCAGCTGCTATGTATTTCGTGTCAAAGAAACTGAAGAAGAAATATAATATTACTGATGAGCGTGCAGCCTTATATGAAGCAGCAGAAACGTGGGTAGATGCTTTGAATGGACGGGACTTCCTAGGAGGCTCAAAACCTAATCTAGCTGATCTTGCGGTGTTCGGGGTGTTAAGACCCATCCGTTACCTGAGATCTGGTAAAGACATGGTGGAGCACACACGTATTGGTGAATGGTACGCCAAAATGGAGAGCATTGTGGGAGACTCCTCCAGGATCAAAGCC

Protein sequence

MRRSSSIASSFLSRSICTAHGSLSATATSNQAALFSTTTTADSYAHRRWFSSLLDSVAGGSARAASFGVVGAVASITAALSMSQEVYAKEPLQQELVPKEVVLYQYEACPFCNKVKAFLDYYDIPYKVVEVNPFSKKEIKWSDYKKVPILVVDGEQLVDSSAIIDQLSHKVLLDRNTDSVSEDDEETKWRRWVDNHLVHVLSPNIYRNTSEALESFDYIASNGNFGAAEKITVKYAGAAAMYFVSKKLKKKYNITDERAALYEAAETWVDALNGRDFLGGSKPNLADLAVFGVLRPIRYLRSGKDMVEHTRIGEWYAKMESIVGDSSRIKA
Homology
[Close]
BLAST of MS002161 vs. NCBI nr
Match: XP_023515439.1 (prostaglandin E synthase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 555.8 bits (1431), Expect = 2.5e-154
Identity = 286/331 (86.40%), Postives = 306/331 (92.45%), Query Frame = 0 

Query: 1   MRRSSSIASSFLSRSICTAHGSLSATATSNQAALFSTTTTADSYAHRRWFSSLLDSVAGG 60
           MRRSSSIASSFL RSI T+HG++SA A SNQ  L +   ++ S+ HRRWFSSLLDSVAGG
Sbjct: 1   MRRSSSIASSFLYRSIGTSHGAVSAYAISNQRLLQAALFSSSSHTHRRWFSSLLDSVAGG 60

Query: 61  SARAASFGVVGAVASITAALSMSQEVYAKEPLQQELVPKEVVLYQYEACPFCNKVKAFLD 120
           SARAAS GVVGAVAS+ AA+SMSQ+VYAKEPL+QELVPKEVVLYQYEACPFCNKVKAFLD
Sbjct: 61  SARAASLGVVGAVASLAAAVSMSQDVYAKEPLRQELVPKEVVLYQYEACPFCNKVKAFLD 120

Query: 121 YYDIPYKVVEVNPFSKKEIKWSDYKKVPILVVDGEQLVDSSAIIDQLSHKVLLDRNTDSV 180
           YYDIPYKVVEVNP SKKEIKWSDYKKVPILVVDGEQ+VDSSAIIDQLSHK L D++  SV
Sbjct: 121 YYDIPYKVVEVNPLSKKEIKWSDYKKVPILVVDGEQMVDSSAIIDQLSHKFLSDKDASSV 180

Query: 181 SEDDEETKWRRWVDNHLVHVLSPNIYRNTSEALESFDYIASNGNFGAAEKITVKYAGAAA 240
            EDDEETKWRRWVD+HLVHVLSPNIYRNTSEALESFDYI SNGNFG AEKI+VKYAGAAA
Sbjct: 181 PEDDEETKWRRWVDSHLVHVLSPNIYRNTSEALESFDYITSNGNFGFAEKISVKYAGAAA 240

Query: 241 MYFVSKKLKKKYNITDERAALYEAAETWVDALNGRDFLGGSKPNLADLAVFGVLRPIRYL 300
           MYFVSKKLKKKYNITDERAALYEAAETWVDAL GRDFLGGSKPNLADLAVFGVLRPIRYL
Sbjct: 241 MYFVSKKLKKKYNITDERAALYEAAETWVDALAGRDFLGGSKPNLADLAVFGVLRPIRYL 300

Query: 301 RSGKDMVEHTRIGEWYAKMESIVGDSSRIKA 332
           RSGKDMVEHTRIGEWY +MES VGDSSR+++
Sbjct: 301 RSGKDMVEHTRIGEWYTRMESTVGDSSRLRS 331

[Close]
BLAST of MS002161 vs. NCBI nr
Match: XP_022988448.1 (prostaglandin E synthase 2-like [Cucurbita maxima])

HSP 1 Score: 555.1 bits (1429), Expect = 4.2e-154
Identity = 287/331 (86.71%), Postives = 307/331 (92.75%), Query Frame = 0 

Query: 1   MRRSSSIASSFLSRSICTAHGSLSATATSNQAALFSTTTTADSYAHRRWFSSLLDSVAGG 60
           MRRSSSIASSFL RSI T+HG++SA A SNQ  L +   ++ S+ HRRWFSSLLDSVAGG
Sbjct: 1   MRRSSSIASSFLYRSIGTSHGAVSAYAISNQRLLQAALFSSSSHTHRRWFSSLLDSVAGG 60

Query: 61  SARAASFGVVGAVASITAALSMSQEVYAKEPLQQELVPKEVVLYQYEACPFCNKVKAFLD 120
           SARAAS GVVGAVAS+ AA+SMSQ+VYAKEPL+QELVPKEVVLYQYEACPFCNKVKAFLD
Sbjct: 61  SARAASLGVVGAVASLAAAVSMSQDVYAKEPLRQELVPKEVVLYQYEACPFCNKVKAFLD 120

Query: 121 YYDIPYKVVEVNPFSKKEIKWSDYKKVPILVVDGEQLVDSSAIIDQLSHKVLLDRNTDSV 180
           YYDIPYKVVEVNP SKKEIKWSDYKKVPILVVDGEQ+VDSSAIIDQLSHK+L D++  SV
Sbjct: 121 YYDIPYKVVEVNPLSKKEIKWSDYKKVPILVVDGEQMVDSSAIIDQLSHKILSDKDA-SV 180

Query: 181 SEDDEETKWRRWVDNHLVHVLSPNIYRNTSEALESFDYIASNGNFGAAEKITVKYAGAAA 240
            EDDEETKWRRWVDNHLVHVLSPNIYRNTSEALESFDYI SNGNFG AEKI+VKYAGAAA
Sbjct: 181 PEDDEETKWRRWVDNHLVHVLSPNIYRNTSEALESFDYITSNGNFGFAEKISVKYAGAAA 240

Query: 241 MYFVSKKLKKKYNITDERAALYEAAETWVDALNGRDFLGGSKPNLADLAVFGVLRPIRYL 300
           MYFVSKKLKKKYNITDERAALYEAAETWVDAL GRDFLGGSKPNLADLAVFGVLRPIRYL
Sbjct: 241 MYFVSKKLKKKYNITDERAALYEAAETWVDALAGRDFLGGSKPNLADLAVFGVLRPIRYL 300

Query: 301 RSGKDMVEHTRIGEWYAKMESIVGDSSRIKA 332
           RSGKDMVEHTRIGEWY +MES VGDSSR+++
Sbjct: 301 RSGKDMVEHTRIGEWYTRMESTVGDSSRLRS 330

[Close]
BLAST of MS002161 vs. NCBI nr
Match: XP_022922089.1 (prostaglandin E synthase 2-like [Cucurbita moschata])

HSP 1 Score: 553.9 bits (1426), Expect = 9.3e-154
Identity = 285/331 (86.10%), Postives = 305/331 (92.15%), Query Frame = 0 

Query: 1   MRRSSSIASSFLSRSICTAHGSLSATATSNQAALFSTTTTADSYAHRRWFSSLLDSVAGG 60
           MRRSSSIASSFL RSI T+HG++SA A SNQ  L +   ++ S+ HRRWFSSLLDSVAGG
Sbjct: 1   MRRSSSIASSFLYRSIGTSHGAVSAYAISNQRLLQAALFSSSSHTHRRWFSSLLDSVAGG 60

Query: 61  SARAASFGVVGAVASITAALSMSQEVYAKEPLQQELVPKEVVLYQYEACPFCNKVKAFLD 120
           S RAAS GVVGAVAS+ AA+SMSQ+VYAKEPL+QELVPKEVVLYQYEACPFCNKVKAFLD
Sbjct: 61  STRAASLGVVGAVASLAAAVSMSQDVYAKEPLRQELVPKEVVLYQYEACPFCNKVKAFLD 120

Query: 121 YYDIPYKVVEVNPFSKKEIKWSDYKKVPILVVDGEQLVDSSAIIDQLSHKVLLDRNTDSV 180
           YYDIPYKVVEVNP SKKEIKWSDYKKVPILVVDGEQ+VDSSAIIDQLSHK L D++  SV
Sbjct: 121 YYDIPYKVVEVNPLSKKEIKWSDYKKVPILVVDGEQMVDSSAIIDQLSHKFLSDQDASSV 180

Query: 181 SEDDEETKWRRWVDNHLVHVLSPNIYRNTSEALESFDYIASNGNFGAAEKITVKYAGAAA 240
            EDDEETKWRRWVD+HLVHVLSPNIYRNTSEALESFDYI SNGNFG AEKI+VKYAGAAA
Sbjct: 181 PEDDEETKWRRWVDSHLVHVLSPNIYRNTSEALESFDYITSNGNFGFAEKISVKYAGAAA 240

Query: 241 MYFVSKKLKKKYNITDERAALYEAAETWVDALNGRDFLGGSKPNLADLAVFGVLRPIRYL 300
           MYFVSKKLKKKYNITDERAALYEAAETWVDAL GRDFLGGSKPNLADLAVFGVLRPIRYL
Sbjct: 241 MYFVSKKLKKKYNITDERAALYEAAETWVDALAGRDFLGGSKPNLADLAVFGVLRPIRYL 300

Query: 301 RSGKDMVEHTRIGEWYAKMESIVGDSSRIKA 332
           RSGKDMVEHTRIGEWY +MES VGDSSR+++
Sbjct: 301 RSGKDMVEHTRIGEWYTRMESTVGDSSRLRS 331

[Close]
BLAST of MS002161 vs. NCBI nr
Match: KAG7023326.1 (Prostaglandin E synthase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 552.4 bits (1422), Expect = 2.7e-153
Identity = 284/331 (85.80%), Postives = 305/331 (92.15%), Query Frame = 0 

Query: 1   MRRSSSIASSFLSRSICTAHGSLSATATSNQAALFSTTTTADSYAHRRWFSSLLDSVAGG 60
           MRRSSSIASSFL RSI T+HG++SA A SNQ  L +   ++ S+ HRRWFSSLLDSVAGG
Sbjct: 1   MRRSSSIASSFLYRSIGTSHGAVSAYAISNQRLLQAALFSSSSHTHRRWFSSLLDSVAGG 60

Query: 61  SARAASFGVVGAVASITAALSMSQEVYAKEPLQQELVPKEVVLYQYEACPFCNKVKAFLD 120
           SARAAS GVVGAVAS+ AA+SMSQ+VYAKEPL+QELVPKEVVLYQYEACPFCNKVKAFLD
Sbjct: 61  SARAASLGVVGAVASLAAAVSMSQDVYAKEPLRQELVPKEVVLYQYEACPFCNKVKAFLD 120

Query: 121 YYDIPYKVVEVNPFSKKEIKWSDYKKVPILVVDGEQLVDSSAIIDQLSHKVLLDRNTDSV 180
           YYDIPYKVVEVNP SKKEIKWSDYKKVPIL+VDGEQ+VDSSAIIDQLSHK L D+   SV
Sbjct: 121 YYDIPYKVVEVNPLSKKEIKWSDYKKVPILLVDGEQMVDSSAIIDQLSHKFLSDKPASSV 180

Query: 181 SEDDEETKWRRWVDNHLVHVLSPNIYRNTSEALESFDYIASNGNFGAAEKITVKYAGAAA 240
            EDDEETKWRRWVD+HLVHVLSPNIYRNTSEALESFDYI SNGNFG AE+I+VKYAGAAA
Sbjct: 181 PEDDEETKWRRWVDSHLVHVLSPNIYRNTSEALESFDYITSNGNFGFAERISVKYAGAAA 240

Query: 241 MYFVSKKLKKKYNITDERAALYEAAETWVDALNGRDFLGGSKPNLADLAVFGVLRPIRYL 300
           MYFVSKKLKKKYNITDERAALYEAAETWVDAL GRDFLGGSKPNLADLAVFGVLRPIRYL
Sbjct: 241 MYFVSKKLKKKYNITDERAALYEAAETWVDALAGRDFLGGSKPNLADLAVFGVLRPIRYL 300

Query: 301 RSGKDMVEHTRIGEWYAKMESIVGDSSRIKA 332
           RSGKDMVEHTRIGEWY +MES VGDSSR+++
Sbjct: 301 RSGKDMVEHTRIGEWYTRMESTVGDSSRLRS 331

[Close]
BLAST of MS002161 vs. NCBI nr
Match: KAG6589638.1 (Prostaglandin E synthase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 550.8 bits (1418), Expect = 7.9e-153
Identity = 283/331 (85.50%), Postives = 304/331 (91.84%), Query Frame = 0 

Query: 1   MRRSSSIASSFLSRSICTAHGSLSATATSNQAALFSTTTTADSYAHRRWFSSLLDSVAGG 60
           MRRSSSIASSF  RSI T+HG++SA A SNQ  L +   ++ S+ HRRWFSSLLDSVAGG
Sbjct: 1   MRRSSSIASSFFYRSIGTSHGAVSAYAISNQRLLQAALFSSSSHTHRRWFSSLLDSVAGG 60

Query: 61  SARAASFGVVGAVASITAALSMSQEVYAKEPLQQELVPKEVVLYQYEACPFCNKVKAFLD 120
           SARAAS GVVGAVAS+ AA+SMSQ+VYAKEPL+QELVPKEVVLYQYEACPFCNKVKAFLD
Sbjct: 61  SARAASLGVVGAVASLAAAVSMSQDVYAKEPLRQELVPKEVVLYQYEACPFCNKVKAFLD 120

Query: 121 YYDIPYKVVEVNPFSKKEIKWSDYKKVPILVVDGEQLVDSSAIIDQLSHKVLLDRNTDSV 180
           YYDIPYKVVEVNP SKKEIKWSDYKKVPIL+VDGEQ+VDSSAIIDQLSHK L D+   SV
Sbjct: 121 YYDIPYKVVEVNPLSKKEIKWSDYKKVPILLVDGEQMVDSSAIIDQLSHKFLSDKPASSV 180

Query: 181 SEDDEETKWRRWVDNHLVHVLSPNIYRNTSEALESFDYIASNGNFGAAEKITVKYAGAAA 240
            EDDEETKWRRWVD+HLVHVLSPNIYRNTSEALESFDYI SNGNFG AE+I+VKYAGAAA
Sbjct: 181 PEDDEETKWRRWVDSHLVHVLSPNIYRNTSEALESFDYITSNGNFGFAERISVKYAGAAA 240

Query: 241 MYFVSKKLKKKYNITDERAALYEAAETWVDALNGRDFLGGSKPNLADLAVFGVLRPIRYL 300
           MYFVSKKLKKKYNITDERAALYEAAETWVDAL GRDFLGGSKPNLADLAVFGVLRPIRYL
Sbjct: 241 MYFVSKKLKKKYNITDERAALYEAAETWVDALAGRDFLGGSKPNLADLAVFGVLRPIRYL 300

Query: 301 RSGKDMVEHTRIGEWYAKMESIVGDSSRIKA 332
           RSGKDMVEHTRIGEWY +MES VGDSSR+++
Sbjct: 301 RSGKDMVEHTRIGEWYTRMESTVGDSSRLRS 331

[Close]
BLAST of MS002161 vs. ExPASy Swiss-Prot
Match: Q7ZUC7 (Prostaglandin E synthase 2 OS=Danio rerio OX=7955 GN=ptges2 PE=2 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 6.5e-57
Identity = 126/289 (43.60%), Postives = 170/289 (58.82%), Query Frame = 0 

Query: 77  TAALSMSQEVYAKEPLQQEL-VPKEVVLYQYEACPFCNKVKAFLDYYDIPYKVVEVNPFS 136
           T  L++   +  KE    +L    ++ LYQY+ CPFC+KV+AFLDY+ +PY++VEVNP  
Sbjct: 82  TIKLTLQHHLAEKESDASDLDTDLKLTLYQYKTCPFCSKVRAFLDYHRLPYEIVEVNPVM 141

Query: 137 KKEIKWSDYKKVPILVVDGE-QLVDSSAIIDQL-------------------SHKVLLDR 196
           ++EIKWS Y+KVPIL+V+G  QL DSS II  L                     K   DR
Sbjct: 142 RQEIKWSTYRKVPILMVNGTVQLNDSSVIISALKTYISSKDKKISEILACYPEMKSKNDR 201

Query: 197 NTDSV----------------------SEDDEETKWRRWVDNHLVHVLSPNIYRNTSEAL 256
             D +                          EE KWR W D+ LVH++SPN+YR  +EAL
Sbjct: 202 GKDVIEFGNKYWVMVHDADADQLYPGKDSRKEEIKWRTWADDWLVHLISPNVYRTPTEAL 261

Query: 257 ESFDYIASNGNFGAAEKITVKYAGAAAMYFVSKKLKKKYNI-TDERAALYEAAETWVDAL 316
            SFDYI   G FG+ E    KY GAAAM+ +SK+LK K+N+  D R  LY+A   WV A+
Sbjct: 262 ASFDYIVREGKFGSFEGFFAKYFGAAAMWIISKRLKYKHNLQADVRQDLYKAVNDWVAAI 321

Query: 317 -NGRDFLGGSKPNLADLAVFGVLRPIRYLRSGKDMVEHTRIGEWYAKME 321
              + F+GG +PNLADLAVFGVLR +  L+S  DM+EHT++ +WY++M+
Sbjct: 322 GKNKQFMGGDEPNLADLAVFGVLRVMEGLQSFDDMMEHTKVKKWYSRMQ 370

[Close]
BLAST of MS002161 vs. ExPASy Swiss-Prot
Match: Q66LN0 (Prostaglandin E synthase 2 OS=Bos taurus OX=9913 GN=PTGES2 PE=1 SV=3)

HSP 1 Score: 218.4 bits (555), Expect = 1.2e-55
Identity = 120/273 (43.96%), Postives = 164/273 (60.07%), Query Frame = 0 

Query: 100 EVVLYQYEACPFCNKVKAFLDYYDIPYKVVEVNPFSKKEIKWSDYKKVPILVV-DGE--- 159
           ++ LYQY+ CPFC+KV+AFLD++ +PY+VVEVNP  + EIK+S Y+KVPI++  +GE   
Sbjct: 98  QLTLYQYKTCPFCSKVRAFLDFHALPYQVVEVNPVRRAEIKFSSYRKVPIVMAQEGESLQ 157

Query: 160 QLVDSSAIIDQL-----------------------------------SHKVLLD-----R 219
           QL DSS II  L                                    + ++LD     R
Sbjct: 158 QLNDSSVIISALKTYLVSGQPLADIITYYPPMKAVNDQGKEVTEFCNKYWLMLDEKEAQR 217

Query: 220 NTDSVSEDDEETKWRRWVDNHLVHVLSPNIYRNTSEALESFDYIASNGNFGAAEKITVKY 279
                    EE KWR+W D+ LVH++SPN+YR  +EAL SFDYI   GNFG  E    KY
Sbjct: 218 MYGGKEARTEEMKWRQWADDWLVHLISPNVYRTPAEALASFDYIVKEGNFGTVEGAMAKY 277

Query: 280 AGAAAMYFVSKKLKKKYNITDE-RAALYEAAETWVDAL-NGRDFLGGSKPNLADLAVFGV 327
            GAAAMYF+SK+LK+++++ D+ R  LYEAA  WV A+   R F+GG KPNLADLAV+GV
Sbjct: 278 MGAAAMYFISKRLKRRHHLRDDVREDLYEAANKWVAAVGKDRPFMGGQKPNLADLAVYGV 337

[Close]
BLAST of MS002161 vs. ExPASy Swiss-Prot
Match: Q8BWM0 (Prostaglandin E synthase 2 OS=Mus musculus OX=10090 GN=Ptges2 PE=1 SV=3)

HSP 1 Score: 214.2 bits (544), Expect = 2.3e-54
Identity = 133/344 (38.66%), Postives = 187/344 (54.36%), Query Frame = 0 

Query: 47  RRWFSSLLDSVAGGSARAASFGVVGAVA-SITAALSM---------SQEVYAKE-----P 106
           R  F+ +     G + R  S  ++GA A ++  AL +         SQ++ A+      P
Sbjct: 35  RAGFTRVAAGSRGAAVRKGSPRLLGAAALALGGALGLYHTVRWHQRSQDLRAERSAAQLP 94

Query: 107 LQQELVPKEVVLYQYEACPFCNKVKAFLDYYDIPYKVVEVNPFSKKEIKWSDYKKVPILV 166
           L   L   ++ LYQY+ CPFC+KV+AFLD++ +PY+VVEVNP  + EIK+S Y+KVPILV
Sbjct: 95  LSNSL---QLTLYQYKTCPFCSKVRAFLDFHSLPYQVVEVNPVRRTEIKFSSYRKVPILV 154

Query: 167 VDG----EQLVDSSAIIDQLSHKVLLDRNTDSV--------------------------- 226
                  +QL DSS II  L   ++  +  + V                           
Sbjct: 155 AQEGDSLQQLNDSSVIISALKTYLVSGQPLEEVITYYPPMKAMNDQGKEVTEFCNKYWLM 214

Query: 227 -------------SEDDEETKWRRWVDNHLVHVLSPNIYRNTSEALESFDYIASNGNFGA 286
                            EE KWR+W D+ LVH++SPN+YR  +EAL SFDYI   G FGA
Sbjct: 215 LDEKEAQQMYGGKEARTEEMKWRQWADDWLVHLISPNVYRTPAEALASFDYIVREGKFGA 274

Query: 287 AEKITVKYAGAAAMYFVSKKLKKKYNITDE-RAALYEAAETWVDAL-NGRDFLGGSKPNL 330
            E    KY GAAAMY +SK+LK ++++ D+ R  LYEAA  WV A+   R F+GG KPNL
Sbjct: 275 VEAAMAKYVGAAAMYLISKRLKSRHHLQDDVRVDLYEAANKWVTAVGKDRPFMGGQKPNL 334

[Close]
BLAST of MS002161 vs. ExPASy Swiss-Prot
Match: Q9H7Z7 (Prostaglandin E synthase 2 OS=Homo sapiens OX=9606 GN=PTGES2 PE=1 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 8.7e-54
Identity = 126/316 (39.87%), Postives = 178/316 (56.33%), Query Frame = 0 

Query: 60  GSARAASFGVVGAVASITAALSMSQEVYAKEPLQQELVPK--EVVLYQYEACPFCNKVKA 119
           G+A  A  G +G   +    L  +Q+++A+    Q  +    ++ LYQY+ CPFC+KV+A
Sbjct: 60  GAAALALGGALGLYHTARWHL-RAQDLHAERSAAQLSLSSRLQLTLYQYKTCPFCSKVRA 119

Query: 120 FLDYYDIPYKVVEVNPFSKKEIKWSDYKKVPILVV----DGEQLVDSSAIIDQLSHKVLL 179
           FLD++ +PY+VVEVNP  + EIK+S Y+KVPILV       +QL DSS II  L   ++ 
Sbjct: 120 FLDFHALPYQVVEVNPVRRAEIKFSSYRKVPILVAQEGESSQQLNDSSVIISALKTYLVS 179

Query: 180 DRNTDSV----------------------------------------SEDDEETKWRRWV 239
            +  + +                                            EE KWR+W 
Sbjct: 180 GQPLEEIITYYPAMKAVNEQGKEVTEFGNKYWLMLNEKEAQQVYGGKEARTEEMKWRQWA 239

Query: 240 DNHLVHVLSPNIYRNTSEALESFDYIASNGNFGAAEKITVKYAGAAAMYFVSKKLKKKYN 299
           D+ LVH++SPN+YR  +EAL SFDYI   G FGA E    KY GAAAMY +SK+LK ++ 
Sbjct: 240 DDWLVHLISPNVYRTPTEALASFDYIVREGKFGAVEGAVAKYMGAAAMYLISKRLKSRHR 299

Query: 300 ITDE-RAALYEAAETWVDAL-NGRDFLGGSKPNLADLAVFGVLRPIRYLRSGKDMVEHTR 328
           + D  R  LYEAA+ WV A+   R F+GG KPNLADLAV+GVLR +  L +  D+++HT 
Sbjct: 300 LQDNVREDLYEAADKWVAAVGKDRPFMGGQKPNLADLAVYGVLRVMEGLDAFDDLMQHTH 359

[Close]
BLAST of MS002161 vs. ExPASy Swiss-Prot
Match: Q9N0A4 (Prostaglandin E synthase 2 OS=Macaca fascicularis OX=9541 GN=PTGES2 PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.1e-53
Identity = 130/316 (41.14%), Postives = 185/316 (58.54%), Query Frame = 0 

Query: 60  GSARAASFGVVGAVASITAALSMSQEVYA-KEPLQQELVPK-EVVLYQYEACPFCNKVKA 119
           G+A  A  G +G   +    L  +Q+++A +  +Q  L  + ++ LYQY+ CPFC+KV+A
Sbjct: 60  GAAALALGGALGLYHTARWHLH-AQDLHAERSAVQLSLSSRLQLTLYQYKTCPFCSKVRA 119

Query: 120 FLDYYDIPYKVVEVNPFSKKEIKWSDYKKVPILVV----DGEQLVDSSAIIDQL------ 179
           FLD++ +PY+VVEVNP  + EIK+S Y+KVPILV       +QL DSS II  L      
Sbjct: 120 FLDFHALPYQVVEVNPVLRAEIKFSSYRKVPILVAQEGESSQQLNDSSVIISALKTYLVS 179

Query: 180 ------------SHKVLLDRNTD----------SVSEDD------------EETKWRRWV 239
                       + K + D+  +           ++E +            EE KWR+W 
Sbjct: 180 GQPLEEIITYYPAMKAVNDQGKEVTEFGNKYWLMLNEKEAQQVYSGKEARTEEMKWRQWA 239

Query: 240 DNHLVHVLSPNIYRNTSEALESFDYIASNGNFGAAEKITVKYAGAAAMYFVSKKLKKKYN 299
           D+ LVH++SPN+YR  +EAL SFDYI   G FGA E    KY GAAAMY +SK+LK ++ 
Sbjct: 240 DDWLVHLISPNVYRTPTEALASFDYIVREGKFGAVEGAVAKYMGAAAMYLISKRLKSRHR 299

Query: 300 ITDE-RAALYEAAETWVDAL-NGRDFLGGSKPNLADLAVFGVLRPIRYLRSGKDMVEHTR 328
           + D  R  LYEAA+ WV A+   R F+GG KPNLADLAV+GVLR +  L +  D+++HT 
Sbjct: 300 LQDNVREDLYEAADKWVAAVGKDRPFMGGQKPNLADLAVYGVLRVMEGLDAFDDLMQHTH 359

[Close]
BLAST of MS002161 vs. ExPASy TrEMBL
Match: A0A6J1JMB5 (Microsomal prostaglandin E synthase 2 OS=Cucurbita maxima OX=3661 GN=LOC111485692 PE=3 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 2.0e-154
Identity = 287/331 (86.71%), Postives = 307/331 (92.75%), Query Frame = 0 

Query: 1   MRRSSSIASSFLSRSICTAHGSLSATATSNQAALFSTTTTADSYAHRRWFSSLLDSVAGG 60
           MRRSSSIASSFL RSI T+HG++SA A SNQ  L +   ++ S+ HRRWFSSLLDSVAGG
Sbjct: 1   MRRSSSIASSFLYRSIGTSHGAVSAYAISNQRLLQAALFSSSSHTHRRWFSSLLDSVAGG 60

Query: 61  SARAASFGVVGAVASITAALSMSQEVYAKEPLQQELVPKEVVLYQYEACPFCNKVKAFLD 120
           SARAAS GVVGAVAS+ AA+SMSQ+VYAKEPL+QELVPKEVVLYQYEACPFCNKVKAFLD
Sbjct: 61  SARAASLGVVGAVASLAAAVSMSQDVYAKEPLRQELVPKEVVLYQYEACPFCNKVKAFLD 120

Query: 121 YYDIPYKVVEVNPFSKKEIKWSDYKKVPILVVDGEQLVDSSAIIDQLSHKVLLDRNTDSV 180
           YYDIPYKVVEVNP SKKEIKWSDYKKVPILVVDGEQ+VDSSAIIDQLSHK+L D++  SV
Sbjct: 121 YYDIPYKVVEVNPLSKKEIKWSDYKKVPILVVDGEQMVDSSAIIDQLSHKILSDKDA-SV 180

Query: 181 SEDDEETKWRRWVDNHLVHVLSPNIYRNTSEALESFDYIASNGNFGAAEKITVKYAGAAA 240
            EDDEETKWRRWVDNHLVHVLSPNIYRNTSEALESFDYI SNGNFG AEKI+VKYAGAAA
Sbjct: 181 PEDDEETKWRRWVDNHLVHVLSPNIYRNTSEALESFDYITSNGNFGFAEKISVKYAGAAA 240

Query: 241 MYFVSKKLKKKYNITDERAALYEAAETWVDALNGRDFLGGSKPNLADLAVFGVLRPIRYL 300
           MYFVSKKLKKKYNITDERAALYEAAETWVDAL GRDFLGGSKPNLADLAVFGVLRPIRYL
Sbjct: 241 MYFVSKKLKKKYNITDERAALYEAAETWVDALAGRDFLGGSKPNLADLAVFGVLRPIRYL 300

Query: 301 RSGKDMVEHTRIGEWYAKMESIVGDSSRIKA 332
           RSGKDMVEHTRIGEWY +MES VGDSSR+++
Sbjct: 301 RSGKDMVEHTRIGEWYTRMESTVGDSSRLRS 330

[Close]
BLAST of MS002161 vs. ExPASy TrEMBL
Match: A0A6J1E2D0 (Microsomal prostaglandin E synthase 2 OS=Cucurbita moschata OX=3662 GN=LOC111430142 PE=3 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 4.5e-154
Identity = 285/331 (86.10%), Postives = 305/331 (92.15%), Query Frame = 0 

Query: 1   MRRSSSIASSFLSRSICTAHGSLSATATSNQAALFSTTTTADSYAHRRWFSSLLDSVAGG 60
           MRRSSSIASSFL RSI T+HG++SA A SNQ  L +   ++ S+ HRRWFSSLLDSVAGG
Sbjct: 1   MRRSSSIASSFLYRSIGTSHGAVSAYAISNQRLLQAALFSSSSHTHRRWFSSLLDSVAGG 60

Query: 61  SARAASFGVVGAVASITAALSMSQEVYAKEPLQQELVPKEVVLYQYEACPFCNKVKAFLD 120
           S RAAS GVVGAVAS+ AA+SMSQ+VYAKEPL+QELVPKEVVLYQYEACPFCNKVKAFLD
Sbjct: 61  STRAASLGVVGAVASLAAAVSMSQDVYAKEPLRQELVPKEVVLYQYEACPFCNKVKAFLD 120

Query: 121 YYDIPYKVVEVNPFSKKEIKWSDYKKVPILVVDGEQLVDSSAIIDQLSHKVLLDRNTDSV 180
           YYDIPYKVVEVNP SKKEIKWSDYKKVPILVVDGEQ+VDSSAIIDQLSHK L D++  SV
Sbjct: 121 YYDIPYKVVEVNPLSKKEIKWSDYKKVPILVVDGEQMVDSSAIIDQLSHKFLSDQDASSV 180

Query: 181 SEDDEETKWRRWVDNHLVHVLSPNIYRNTSEALESFDYIASNGNFGAAEKITVKYAGAAA 240
            EDDEETKWRRWVD+HLVHVLSPNIYRNTSEALESFDYI SNGNFG AEKI+VKYAGAAA
Sbjct: 181 PEDDEETKWRRWVDSHLVHVLSPNIYRNTSEALESFDYITSNGNFGFAEKISVKYAGAAA 240

Query: 241 MYFVSKKLKKKYNITDERAALYEAAETWVDALNGRDFLGGSKPNLADLAVFGVLRPIRYL 300
           MYFVSKKLKKKYNITDERAALYEAAETWVDAL GRDFLGGSKPNLADLAVFGVLRPIRYL
Sbjct: 241 MYFVSKKLKKKYNITDERAALYEAAETWVDALAGRDFLGGSKPNLADLAVFGVLRPIRYL 300

Query: 301 RSGKDMVEHTRIGEWYAKMESIVGDSSRIKA 332
           RSGKDMVEHTRIGEWY +MES VGDSSR+++
Sbjct: 301 RSGKDMVEHTRIGEWYTRMESTVGDSSRLRS 331

[Close]
BLAST of MS002161 vs. ExPASy TrEMBL
Match: A0A5D3DXB5 (Microsomal prostaglandin E synthase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G001320 PE=3 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 1.9e-152
Identity = 288/333 (86.49%), Postives = 306/333 (91.89%), Query Frame = 0 

Query: 1   MRRSSSIASSFLSRSICTAHGSLSATATSN--QAALFSTTTTADSYAHRRWFSSLLDSVA 60
           MRRSSSIASSFLSRSI T+H S SA ++    QAALFS+T    S+ HRRWFSSLLDS++
Sbjct: 1   MRRSSSIASSFLSRSISTSHAS-SAISSHRLLQAALFSST----SHTHRRWFSSLLDSLS 60

Query: 61  GGSARAASFGVVGAVASITAALSMSQEVYAKEPLQQELVPKEVVLYQYEACPFCNKVKAF 120
           GGS RA S GVVGAVASI AA+SMSQEVYA+E L+QEL+PKEVVLYQYEACPFCNKVKAF
Sbjct: 61  GGSTRAVSLGVVGAVASIAAAVSMSQEVYAEERLRQELIPKEVVLYQYEACPFCNKVKAF 120

Query: 121 LDYYDIPYKVVEVNPFSKKEIKWSDYKKVPILVVDGEQLVDSSAIIDQLSHKVLLDRNTD 180
           LDYYDIPYKVVEVNP SKKEIKWSDYKKVPILVVDGEQLVDSSAIIDQLSH+VL D+N  
Sbjct: 121 LDYYDIPYKVVEVNPLSKKEIKWSDYKKVPILVVDGEQLVDSSAIIDQLSHRVLSDKNVS 180

Query: 181 SVSEDDEETKWRRWVDNHLVHVLSPNIYRNTSEALESFDYIASNGNFGAAEKITVKYAGA 240
           SVSEDDEETKWRRWVDNHLVH+LSPNIYRNTSEALESFDYI SNGNFG AEKI+VKYAGA
Sbjct: 181 SVSEDDEETKWRRWVDNHLVHILSPNIYRNTSEALESFDYITSNGNFGFAEKISVKYAGA 240

Query: 241 AAMYFVSKKLKKKYNITDERAALYEAAETWVDALNGRDFLGGSKPNLADLAVFGVLRPIR 300
           AAMYFVSKKLKKKYNITDERAALYEAAETWVDAL GRDFLGGSKPNLADLAVFGVLRPIR
Sbjct: 241 AAMYFVSKKLKKKYNITDERAALYEAAETWVDALAGRDFLGGSKPNLADLAVFGVLRPIR 300

Query: 301 YLRSGKDMVEHTRIGEWYAKMESIVGDSSRIKA 332
           YLRSGKDMVEHTRIGEWY +MES VGDSSRI++
Sbjct: 301 YLRSGKDMVEHTRIGEWYTRMESAVGDSSRIRS 328

[Close]
BLAST of MS002161 vs. ExPASy TrEMBL
Match: A0A1S3BWC8 (Microsomal prostaglandin E synthase 2 OS=Cucumis melo OX=3656 GN=LOC103494185 PE=3 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 1.9e-152
Identity = 288/333 (86.49%), Postives = 306/333 (91.89%), Query Frame = 0 

Query: 1   MRRSSSIASSFLSRSICTAHGSLSATATSN--QAALFSTTTTADSYAHRRWFSSLLDSVA 60
           MRRSSSIASSFLSRSI T+H S SA ++    QAALFS+T    S+ HRRWFSSLLDS++
Sbjct: 1   MRRSSSIASSFLSRSISTSHAS-SAISSHRLLQAALFSST----SHTHRRWFSSLLDSLS 60

Query: 61  GGSARAASFGVVGAVASITAALSMSQEVYAKEPLQQELVPKEVVLYQYEACPFCNKVKAF 120
           GGS RA S GVVGAVASI AA+SMSQEVYA+E L+QEL+PKEVVLYQYEACPFCNKVKAF
Sbjct: 61  GGSTRAVSLGVVGAVASIAAAVSMSQEVYAEERLRQELIPKEVVLYQYEACPFCNKVKAF 120

Query: 121 LDYYDIPYKVVEVNPFSKKEIKWSDYKKVPILVVDGEQLVDSSAIIDQLSHKVLLDRNTD 180
           LDYYDIPYKVVEVNP SKKEIKWSDYKKVPILVVDGEQLVDSSAIIDQLSH+VL D+N  
Sbjct: 121 LDYYDIPYKVVEVNPLSKKEIKWSDYKKVPILVVDGEQLVDSSAIIDQLSHRVLSDKNVS 180

Query: 181 SVSEDDEETKWRRWVDNHLVHVLSPNIYRNTSEALESFDYIASNGNFGAAEKITVKYAGA 240
           SVSEDDEETKWRRWVDNHLVH+LSPNIYRNTSEALESFDYI SNGNFG AEKI+VKYAGA
Sbjct: 181 SVSEDDEETKWRRWVDNHLVHILSPNIYRNTSEALESFDYITSNGNFGFAEKISVKYAGA 240

Query: 241 AAMYFVSKKLKKKYNITDERAALYEAAETWVDALNGRDFLGGSKPNLADLAVFGVLRPIR 300
           AAMYFVSKKLKKKYNITDERAALYEAAETWVDAL GRDFLGGSKPNLADLAVFGVLRPIR
Sbjct: 241 AAMYFVSKKLKKKYNITDERAALYEAAETWVDALAGRDFLGGSKPNLADLAVFGVLRPIR 300

Query: 301 YLRSGKDMVEHTRIGEWYAKMESIVGDSSRIKA 332
           YLRSGKDMVEHTRIGEWY +MES VGDSSRI++
Sbjct: 301 YLRSGKDMVEHTRIGEWYTRMESAVGDSSRIRS 328

[Close]
BLAST of MS002161 vs. ExPASy TrEMBL
Match: A0A5A7UQJ9 (Microsomal prostaglandin E synthase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G004530 PE=3 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 6.1e-151
Identity = 287/333 (86.19%), Postives = 305/333 (91.59%), Query Frame = 0 

Query: 1   MRRSSSIASSFLSRSICTAHGSLSATATSN--QAALFSTTTTADSYAHRRWFSSLLDSVA 60
           MRRSSSIASSFLSRSI T+HGS SA ++    QAALFS+T    S+ HRR FSSLLD ++
Sbjct: 1   MRRSSSIASSFLSRSISTSHGS-SAISSHRLLQAALFSST----SHTHRRCFSSLLDFLS 60

Query: 61  GGSARAASFGVVGAVASITAALSMSQEVYAKEPLQQELVPKEVVLYQYEACPFCNKVKAF 120
           GGS RA S GVVGAVASI AA+SMSQEVYA+E L+QEL+PKEVVLYQYEACPFCNKVKAF
Sbjct: 61  GGSTRAVSLGVVGAVASIAAAVSMSQEVYAEERLRQELIPKEVVLYQYEACPFCNKVKAF 120

Query: 121 LDYYDIPYKVVEVNPFSKKEIKWSDYKKVPILVVDGEQLVDSSAIIDQLSHKVLLDRNTD 180
           LDYYDIPYKVVEVNP SKKEIKWSDYKKVPILVVDGEQLVDSSAIIDQLSH+VL D+N  
Sbjct: 121 LDYYDIPYKVVEVNPLSKKEIKWSDYKKVPILVVDGEQLVDSSAIIDQLSHRVLSDKNVS 180

Query: 181 SVSEDDEETKWRRWVDNHLVHVLSPNIYRNTSEALESFDYIASNGNFGAAEKITVKYAGA 240
           SVSEDDEETKWRRWVDNHLVH+LSPNIYRNTSEALESFDYI SNGNFG AEKI+VKYAGA
Sbjct: 181 SVSEDDEETKWRRWVDNHLVHILSPNIYRNTSEALESFDYITSNGNFGFAEKISVKYAGA 240

Query: 241 AAMYFVSKKLKKKYNITDERAALYEAAETWVDALNGRDFLGGSKPNLADLAVFGVLRPIR 300
           AAMYFVSKKLKKKYNITDERAALYEAAETWVDAL GRDFLGGSKPNLADLAVFGVLRPIR
Sbjct: 241 AAMYFVSKKLKKKYNITDERAALYEAAETWVDALAGRDFLGGSKPNLADLAVFGVLRPIR 300

Query: 301 YLRSGKDMVEHTRIGEWYAKMESIVGDSSRIKA 332
           YLRSGKDMVEHTRIGEWY +MES VGDSSRI++
Sbjct: 301 YLRSGKDMVEHTRIGEWYTRMESAVGDSSRIRS 328

[Close]
BLAST of MS002161 vs. TAIR 10
Match: AT5G42150.1 (Glutathione S-transferase family protein )

HSP 1 Score: 390.2 bits (1001), Expect = 1.7e-108
Identity = 217/334 (64.97%), Postives = 250/334 (74.85%), Query Frame = 0 

Query: 1   MRRSSSIASSFLSRSICTAHGSLSATATSNQAALFSTTTTADSYAHRRWFSSLLD----S 60
           MRR + +A+  +S S+     ++++  T + A     TT + S    R F  L +    S
Sbjct: 1   MRRVTGLAARTISSSV-----AINSRLTQSMA----ITTISSSEPISRRFGGLPEIKTPS 60

Query: 61  VAGGSARAASFGVVGAVASITAALSMSQEVYAKEPLQQELVPKEVVLYQYEACPFCNKVK 120
            AGG A    F       S  A  S+ QEV+AKE + Q+  PKEVVLYQYEACPFCNKVK
Sbjct: 61  FAGGVAGVVFF-------SAAAVSSLGQEVHAKE-MAQKFNPKEVVLYQYEACPFCNKVK 120

Query: 121 AFLDYYDIPYKVVEVNPFSKKEIKWSDYKKVPILVVDGEQLVDSSAIIDQLSHKVLLDRN 180
           AFLDY  IPYKVVEVNP SKKEIKWSDYKKVPIL VDGEQ+VDSS IID L  K+     
Sbjct: 121 AFLDYNKIPYKVVEVNPISKKEIKWSDYKKVPILTVDGEQMVDSSVIIDSLFQKM---HP 180

Query: 181 TDSVSEDDEETKWRRWVDNHLVHVLSPNIYRNTSEALESFDYIASNGNFGAAEKITVKYA 240
             S SEDDEETKWR+WVDNHLVH+LSPNIYRNTSEALESF+YI ++GNF   E++  KYA
Sbjct: 181 EISKSEDDEETKWRKWVDNHLVHLLSPNIYRNTSEALESFEYITTHGNFSFTERLVAKYA 240

Query: 241 GAAAMYFVSKKLKKKYNITDERAALYEAAETWVDALNGRDFLGGSKPNLADLAVFGVLRP 300
           GA AMYFVSKKLKKKYNITDERAALY+AAETWVDAL  R +LGGSKPNL DLAVFGVLRP
Sbjct: 241 GATAMYFVSKKLKKKYNITDERAALYDAAETWVDALKERPYLGGSKPNLGDLAVFGVLRP 300

Query: 301 IRYLRSGKDMVEHTRIGEWYAKMESIVGDSSRIK 331
           IRYLRSGKDMV++TRIGEWY++ME+ VG+ SRIK
Sbjct: 301 IRYLRSGKDMVDNTRIGEWYSRMENTVGEPSRIK 314

The following BLAST results are available for this feature:
BLAST of MS002161 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023515439.12.5e-15486.40prostaglandin E synthase 2-like [Cucurbita pepo subsp. pepo][more]
XP_022988448.14.2e-15486.71prostaglandin E synthase 2-like [Cucurbita maxima][more]
XP_022922089.19.3e-15486.10prostaglandin E synthase 2-like [Cucurbita moschata][more]
KAG7023326.12.7e-15385.80Prostaglandin E synthase 2 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6589638.17.9e-15385.50Prostaglandin E synthase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
BLAST of MS002161 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q7ZUC76.5e-5743.60Prostaglandin E synthase 2 OS=Danio rerio OX=7955 GN=ptges2 PE=2 SV=1[more]
Q66LN01.2e-5543.96Prostaglandin E synthase 2 OS=Bos taurus OX=9913 GN=PTGES2 PE=1 SV=3[more]
Q8BWM02.3e-5438.66Prostaglandin E synthase 2 OS=Mus musculus OX=10090 GN=Ptges2 PE=1 SV=3[more]
Q9H7Z78.7e-5439.87Prostaglandin E synthase 2 OS=Homo sapiens OX=9606 GN=PTGES2 PE=1 SV=1[more]
Q9N0A41.1e-5341.14Prostaglandin E synthase 2 OS=Macaca fascicularis OX=9541 GN=PTGES2 PE=1 SV=1[more]
BLAST of MS002161 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1JMB52.0e-15486.71Microsomal prostaglandin E synthase 2 OS=Cucurbita maxima OX=3661 GN=LOC11148569... [more]
A0A6J1E2D04.5e-15486.10Microsomal prostaglandin E synthase 2 OS=Cucurbita moschata OX=3662 GN=LOC111430... [more]
A0A5D3DXB51.9e-15286.49Microsomal prostaglandin E synthase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3BWC81.9e-15286.49Microsomal prostaglandin E synthase 2 OS=Cucumis melo OX=3656 GN=LOC103494185 PE... [more]
A0A5A7UQJ96.1e-15186.19Microsomal prostaglandin E synthase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
BLAST of MS002161 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G42150.11.7e-10864.97Glutathione S-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 92..173
e-value: 4.9E-32
score: 111.5
NoneNo IPR availableGENE3D1.20.1050.10coord: 179..329
e-value: 7.9E-65
score: 219.1
NoneNo IPR availableSFLDSFLDG01182Prostaglandin_E_synthase_likecoord: 27..330
e-value: 0.0
score: 425.8
NoneNo IPR availablePANTHERPTHR12782:SF12PROSTAGLANDIN E SYNTHASE 2-RELATEDcoord: 1..331
NoneNo IPR availablePANTHERPTHR12782MICROSOMAL PROSTAGLANDIN E SYNTHASE-2coord: 1..331
NoneNo IPR availablePROSITEPS51354GLUTAREDOXIN_2coord: 89..184
score: 13.960805
NoneNo IPR availableCDDcd03040GST_N_mPGES2coord: 100..171
e-value: 4.36329E-41
score: 136.384
IPR004045Glutathione S-transferase, N-terminalPFAMPF13417GST_N_3coord: 103..169
e-value: 2.4E-14
score: 53.4
IPR004045Glutathione S-transferase, N-terminalPROSITEPS50404GST_NTERcoord: 99..175
score: 14.002681
IPR034334Prostaglandin E synthase 2SFLDSFLDG01203Prostaglandin_E_synthase_likecoord: 27..330
e-value: 0.0
score: 425.8
IPR011767Glutaredoxin active sitePROSITEPS00195GLUTAREDOXIN_1coord: 103..119
IPR034335Prostaglandin E synthase 2, C-terminalCDDcd03197GST_C_mPGES2coord: 182..324
e-value: 1.66342E-76
score: 229.415
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 100..197
IPR036282Glutathione S-transferase, C-terminal domain superfamilySUPERFAMILY47616GST C-terminal domain-likecoord: 182..323

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002161.1MS002161.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006749 glutathione metabolic process
molecular_function GO:0004362 glutathione-disulfide reductase (NADPH) activity
molecular_function GO:0050220 prostaglandin-E synthase activity
molecular_function GO:0005515 protein binding