MS002152 (gene) Bitter gourd (TR) v1

Overview


Name	MS002152
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Calcium-binding EF-hand family protein
Location	scaffold30: 2794544 .. 2794981 (-)
RNA-Seq Expression	MS002152
Synteny	MS002152

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GACAGCGAGGAACTGCGCAAGGTTTTCGACCGCTTCGATGCCAATGGCGACGGCAAGATCTCCATCTCCGAGCTCGATTCCGTCCTCAAGACCTTGGGATCCTCCATCCCATACGATGAGCTCCGCCGCGTCATGGAGGATCTCGACTCTGACAAGGACGGGTTCATCAACATCGACGAGTTCGCCGCCTTCTGCAAGGAACCGATGGCCTCCGACGAAGCCGGTGCCGCCGAGCTTCGCGACGCCTTTGACCTCTACGATCAGGACCACAACGGCCTCATCTCCCACTCCGAGCTCCACCTCGTCCTTAATCGCCTCGGAATCACCTGCACCAAGGAGGACTGTCAACGCATGATCTGCTCCGTCGATTCCGATGGCGATGGAAATGTCAATTTCGAGGAGTTCAGGAAGATGATGACCGCTAATTCCAACTCCAAT

mRNA sequence

GACAGCGAGGAACTGCGCAAGGTTTTCGACCGCTTCGATGCCAATGGCGACGGCAAGATCTCCATCTCCGAGCTCGATTCCGTCCTCAAGACCTTGGGATCCTCCATCCCATACGATGAGCTCCGCCGCGTCATGGAGGATCTCGACTCTGACAAGGACGGGTTCATCAACATCGACGAGTTCGCCGCCTTCTGCAAGGAACCGATGGCCTCCGACGAAGCCGGTGCCGCCGAGCTTCGCGACGCCTTTGACCTCTACGATCAGGACCACAACGGCCTCATCTCCCACTCCGAGCTCCACCTCGTCCTTAATCGCCTCGGAATCACCTGCACCAAGGAGGACTGTCAACGCATGATCTGCTCCGTCGATTCCGATGGCGATGGAAATGTCAATTTCGAGGAGTTCAGGAAGATGATGACCGCTAATTCCAACTCCAAT

Coding sequence (CDS)

GACAGCGAGGAACTGCGCAAGGTTTTCGACCGCTTCGATGCCAATGGCGACGGCAAGATCTCCATCTCCGAGCTCGATTCCGTCCTCAAGACCTTGGGATCCTCCATCCCATACGATGAGCTCCGCCGCGTCATGGAGGATCTCGACTCTGACAAGGACGGGTTCATCAACATCGACGAGTTCGCCGCCTTCTGCAAGGAACCGATGGCCTCCGACGAAGCCGGTGCCGCCGAGCTTCGCGACGCCTTTGACCTCTACGATCAGGACCACAACGGCCTCATCTCCCACTCCGAGCTCCACCTCGTCCTTAATCGCCTCGGAATCACCTGCACCAAGGAGGACTGTCAACGCATGATCTGCTCCGTCGATTCCGATGGCGATGGAAATGTCAATTTCGAGGAGTTCAGGAAGATGATGACCGCTAATTCCAACTCCAAT

Protein sequence

DSEELRKVFDRFDANGDGKISISELDSVLKTLGSSIPYDELRRVMEDLDSDKDGFINIDEFAAFCKEPMASDEAGAAELRDAFDLYDQDHNGLISHSELHLVLNRLGITCTKEDCQRMICSVDSDGDGNVNFEEFRKMMTANSNSN

Homology

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BLAST of MS002152 vs. NCBI nr
Match: XP_038878467.1 (probable calcium-binding protein CML27 [Benincasa hispida])

HSP 1 Score: 274.6 bits (701), Expect = 4.8e-70
Identity = 135/145 (93.10%), Postives = 143/145 (98.62%), Query Frame = 0


Query: 1   DSEELRKVFDRFDANGDGKISISELDSVLKTLGSSIPYDELRRVMEDLDSDKDGFINIDE 60
           D+EELRKVF+RFDANGDGKISISELDSVLK+LGSSIPY+ELR VMEDLDSDKDG+INIDE
Sbjct: 29  DNEELRKVFERFDANGDGKISISELDSVLKSLGSSIPYEELRSVMEDLDSDKDGYINIDE 88

Query: 61  FAAFCKEPMASDEAGAAELRDAFDLYDQDHNGLISHSELHLVLNRLGITCTKEDCQRMIC 120
           FAAFCKEPMASDEAGA+ELRDAFDLYDQDHNGLISHSELHLVLNRLGI+C+KEDCQRMI 
Sbjct: 89  FAAFCKEPMASDEAGASELRDAFDLYDQDHNGLISHSELHLVLNRLGISCSKEDCQRMIH 148

Query: 121 SVDSDGDGNVNFEEFRKMMTANSNS 146
           SVDSDGDGNVNFEEFRKMMTANSNS
Sbjct: 149 SVDSDGDGNVNFEEFRKMMTANSNS 173

[Close]

BLAST of MS002152 vs. NCBI nr
Match: XP_023529417.1 (probable calcium-binding protein CML27 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 265.4 bits (677), Expect = 2.9e-67
Identity = 130/145 (89.66%), Postives = 139/145 (95.86%), Query Frame = 0


Query: 1   DSEELRKVFDRFDANGDGKISISELDSVLKTLGSSIPYDELRRVMEDLDSDKDGFINIDE 60
           D+ ELRKVFDRFDANGDGKIS+SELDSVLK+LGSS+PYDELR VMEDLD+DKDGFINIDE
Sbjct: 29  DNAELRKVFDRFDANGDGKISLSELDSVLKSLGSSVPYDELRAVMEDLDTDKDGFINIDE 88

Query: 61  FAAFCKEPMASDEAGAAELRDAFDLYDQDHNGLISHSELHLVLNRLGITCTKEDCQRMIC 120
           FA FCKEPMAS+EAGA+ELRDAFDLYDQDHNGLIS SELHLVLNRLGITC+KEDCQRMI 
Sbjct: 89  FAVFCKEPMASNEAGASELRDAFDLYDQDHNGLISQSELHLVLNRLGITCSKEDCQRMIH 148

Query: 121 SVDSDGDGNVNFEEFRKMMTANSNS 146
           SVDSDGDGNVNFEEFRKMM+ NSNS
Sbjct: 149 SVDSDGDGNVNFEEFRKMMSNNSNS 173

[Close]

BLAST of MS002152 vs. NCBI nr
Match: XP_022933917.1 (probable calcium-binding protein CML27 [Cucurbita moschata] >KAG7021647.1 putative calcium-binding protein CML27, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 265.4 bits (677), Expect = 2.9e-67
Identity = 130/145 (89.66%), Postives = 139/145 (95.86%), Query Frame = 0


Query: 1   DSEELRKVFDRFDANGDGKISISELDSVLKTLGSSIPYDELRRVMEDLDSDKDGFINIDE 60
           D+ ELRKVFDRFDANGDGKIS+SELDSVLK+LGSS+PYDELR VMEDLD+DKDGFINIDE
Sbjct: 29  DNAELRKVFDRFDANGDGKISLSELDSVLKSLGSSVPYDELRAVMEDLDTDKDGFINIDE 88

Query: 61  FAAFCKEPMASDEAGAAELRDAFDLYDQDHNGLISHSELHLVLNRLGITCTKEDCQRMIC 120
           FA FCKEPMAS+EAGA+ELRDAFDLYDQDHNGLIS SELHLVLNRLGITC+KEDCQRMI 
Sbjct: 89  FAVFCKEPMASNEAGASELRDAFDLYDQDHNGLISQSELHLVLNRLGITCSKEDCQRMIH 148

Query: 121 SVDSDGDGNVNFEEFRKMMTANSNS 146
           SVDSDGDGNVNFEEFRKMM+ NSNS
Sbjct: 149 SVDSDGDGNVNFEEFRKMMSNNSNS 173

[Close]

BLAST of MS002152 vs. NCBI nr
Match: KAG6587683.1 (putative calcium-binding protein CML27, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 261.9 bits (668), Expect = 3.2e-66
Identity = 129/145 (88.97%), Postives = 138/145 (95.17%), Query Frame = 0


Query: 1   DSEELRKVFDRFDANGDGKISISELDSVLKTLGSSIPYDELRRVMEDLDSDKDGFINIDE 60
           D+ ELRKVFDRFDANGDGKIS+SELDSVLK+LGSS+P DELR VMEDLD+DKDGFINIDE
Sbjct: 29  DNAELRKVFDRFDANGDGKISLSELDSVLKSLGSSVPNDELRAVMEDLDTDKDGFINIDE 88

Query: 61  FAAFCKEPMASDEAGAAELRDAFDLYDQDHNGLISHSELHLVLNRLGITCTKEDCQRMIC 120
           FA FCKEPMAS+EAGA+ELRDAFDLYDQDHNGLIS SELHLVLNRLGITC+KEDCQRMI 
Sbjct: 89  FAVFCKEPMASNEAGASELRDAFDLYDQDHNGLISQSELHLVLNRLGITCSKEDCQRMIH 148

Query: 121 SVDSDGDGNVNFEEFRKMMTANSNS 146
           SVDSDGDGNVNFEEFRKMM+ NSNS
Sbjct: 149 SVDSDGDGNVNFEEFRKMMSNNSNS 173

[Close]

BLAST of MS002152 vs. NCBI nr
Match: XP_023003784.1 (probable calcium-binding protein CML27 [Cucurbita maxima])

HSP 1 Score: 261.5 bits (667), Expect = 4.2e-66
Identity = 128/145 (88.28%), Postives = 138/145 (95.17%), Query Frame = 0


Query: 1   DSEELRKVFDRFDANGDGKISISELDSVLKTLGSSIPYDELRRVMEDLDSDKDGFINIDE 60
           D+ ELRKVFDRFDANGDGKIS+SELDSVLK+ GSS+PYDELR VMEDLD+DKDGFINIDE
Sbjct: 29  DNAELRKVFDRFDANGDGKISLSELDSVLKSSGSSVPYDELRAVMEDLDTDKDGFINIDE 88

Query: 61  FAAFCKEPMASDEAGAAELRDAFDLYDQDHNGLISHSELHLVLNRLGITCTKEDCQRMIC 120
           FA FCKEP+AS+EAGA+ELRDAFDLYDQDHNGLIS SELHLVLNRLGITC+KEDCQRMI 
Sbjct: 89  FAVFCKEPIASNEAGASELRDAFDLYDQDHNGLISQSELHLVLNRLGITCSKEDCQRMIH 148

Query: 121 SVDSDGDGNVNFEEFRKMMTANSNS 146
           SVDSDGDGNVNFEEFRKMM+ NSNS
Sbjct: 149 SVDSDGDGNVNFEEFRKMMSNNSNS 173

[Close]

BLAST of MS002152 vs. ExPASy Swiss-Prot
Match: Q9LE22 (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 1.7e-46
Identity = 93/146 (63.70%), Postives = 117/146 (80.14%), Query Frame = 0


Query: 3   EELRKVFDRFDANGDGKISISELDSVLKTLGSSIPYDELRRVMEDLDSDKDGFINIDEFA 62
           EEL+KVFD+FD+NGDGKIS+ EL  V K +G+S    EL RV+E++D+D+DG+IN+DEF+
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 63  AFCKEPMASDEAGAAELRDAFDLYDQDHNGLISHSELHLVLNRLGITCTKEDCQRMICSV 122
             C+       + AAE+RDAFDLYDQD NGLIS SELH VLNRLG++C+ EDC RMI  V
Sbjct: 82  TLCR-----SSSSAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPV 141

Query: 123 DSDGDGNVNFEEFRKMMTANS--NSN 147
           D+DGDGNVNFEEF+KMMT++S  NSN
Sbjct: 142 DADGDGNVNFEEFQKMMTSSSLLNSN 162

[Close]

BLAST of MS002152 vs. ExPASy Swiss-Prot
Match: Q9C9U8 (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 PE=1 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 3.6e-44
Identity = 86/138 (62.32%), Postives = 111/138 (80.43%), Query Frame = 0


Query: 4   ELRKVFDRFDANGDGKISISELDSVLKTLGSSIPYDELRRVMEDLDSDKDGFINIDEFAA 63
           EL+KVFD+FDANGDGKIS+SEL +V K++G+S   +EL RV++++D D DGFIN +EFA 
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 64  FCKEPMASDEAGAAELRDAFDLYDQDHNGLISHSELHLVLNRLGITCTKEDCQRMICSVD 123
            C+       + A E+R+AFDLYDQ+ NGLIS SE+H VLNRLG+TC+ EDC RMI  VD
Sbjct: 80  ICR-----SSSSAVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVD 139

Query: 124 SDGDGNVNFEEFRKMMTA 142
           +DGDGNVNFEEF+KMM++
Sbjct: 140 TDGDGNVNFEEFQKMMSS 152

[Close]

BLAST of MS002152 vs. ExPASy Swiss-Prot
Match: Q9M7R0 (Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 1.1e-40
Identity = 88/149 (59.06%), Postives = 109/149 (73.15%), Query Frame = 0


Query: 1   DSEELRKVFDRFDANGDGKISISELDSVLKTLGSSIPYDELRRVMEDLDSDKDGFINIDE 60
           +  E++ VF+RFDANGDGKIS  EL  VLK LGS+   +E+ R+ME++D+DKDGFIN+ E
Sbjct: 17  EPNEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTDKDGFINVQE 76

Query: 61  FAAFCK---EPMASDEAGAAELRDAFDLYDQDHNGLISHSELHLVLNRLGITCTKEDCQR 120
           FAAF K   +P  S   G  EL++AF+LYDQDHNGLIS  ELH +L RLG    + DC  
Sbjct: 77  FAAFVKAETDPYPS-SGGENELKEAFELYDQDHNGLISSVELHKILTRLGERYAEHDCVE 136

Query: 121 MICSVDSDGDGNVNFEEFRKMMTANSNSN 147
           MI SVDSDGDG V+FEEF+KMMT  S +N
Sbjct: 137 MIKSVDSDGDGYVSFEEFKKMMTNKSGNN 164

[Close]

BLAST of MS002152 vs. ExPASy Swiss-Prot
Match: Q0DJV6 (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 8.1e-36
Identity = 79/147 (53.74%), Postives = 102/147 (69.39%), Query Frame = 0


Query: 4   ELRKVFDRFDANGDGKISISELDSVLKTLGSSIPYDELRRVMEDLDSDKDGFINIDEFAA 63
           EL +VF R+DANGDGKIS  EL SVL+ LG+ +   E+RR+M+++DSD+DGF+++ EFAA
Sbjct: 5   ELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEFAA 64

Query: 64  FCKEPMAS-----------DEAGAAELRDAFDLYDQDHNGLISHSELHLVLNRLGITCTK 123
           F   P  +           + A  AELR+AF +YD D NG IS  ELH VL +LG  C+ 
Sbjct: 65  FHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKCSV 124

Query: 124 EDCQRMICSVDSDGDGNVNFEEFRKMM 140
            DC RMI SVD+DGDG VNF+EF+KMM
Sbjct: 125 ADCSRMIRSVDADGDGCVNFDEFKKMM 151

[Close]

BLAST of MS002152 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 2.9e-33
Identity = 72/144 (50.00%), Postives = 99/144 (68.75%), Query Frame = 0


Query: 3   EELRKVFDRFDANGDGKISISELDSVLKTLGSSIPYDELRRVMEDLDSDKDGFINIDEFA 62
           E+++KVF RFD N DGKISI EL  V+  L  +   +E + +M++ D D +GFI++DEF 
Sbjct: 14  EDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFV 73

Query: 63  A-FCKEPMASDEAGAAELRDAFDLYDQDHNGLISHSELHLVLNRLGITCTKEDCQRMICS 122
           A F     +S+ +   +L++AFDLYD D NG IS +ELH V+  LG  C+ +DCQRMI  
Sbjct: 74  ALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINK 133

Query: 123 VDSDGDGNVNFEEFRKMMTANSNS 146
           VDSDGDG V+FEEF+KMM  N ++
Sbjct: 134 VDSDGDGCVDFEEFKKMMMINGSA 157

[Close]

BLAST of MS002152 vs. ExPASy TrEMBL
Match: A0A6J1F164 (probable calcium-binding protein CML27 OS=Cucurbita moschata OX=3662 GN=LOC111441185 PE=4 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.4e-67
Identity = 130/145 (89.66%), Postives = 139/145 (95.86%), Query Frame = 0


Query: 1   DSEELRKVFDRFDANGDGKISISELDSVLKTLGSSIPYDELRRVMEDLDSDKDGFINIDE 60
           D+ ELRKVFDRFDANGDGKIS+SELDSVLK+LGSS+PYDELR VMEDLD+DKDGFINIDE
Sbjct: 29  DNAELRKVFDRFDANGDGKISLSELDSVLKSLGSSVPYDELRAVMEDLDTDKDGFINIDE 88

Query: 61  FAAFCKEPMASDEAGAAELRDAFDLYDQDHNGLISHSELHLVLNRLGITCTKEDCQRMIC 120
           FA FCKEPMAS+EAGA+ELRDAFDLYDQDHNGLIS SELHLVLNRLGITC+KEDCQRMI 
Sbjct: 89  FAVFCKEPMASNEAGASELRDAFDLYDQDHNGLISQSELHLVLNRLGITCSKEDCQRMIH 148

Query: 121 SVDSDGDGNVNFEEFRKMMTANSNS 146
           SVDSDGDGNVNFEEFRKMM+ NSNS
Sbjct: 149 SVDSDGDGNVNFEEFRKMMSNNSNS 173

[Close]

BLAST of MS002152 vs. ExPASy TrEMBL
Match: A0A6J1KUB4 (probable calcium-binding protein CML27 OS=Cucurbita maxima OX=3661 GN=LOC111497259 PE=4 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 2.0e-66
Identity = 128/145 (88.28%), Postives = 138/145 (95.17%), Query Frame = 0


Query: 1   DSEELRKVFDRFDANGDGKISISELDSVLKTLGSSIPYDELRRVMEDLDSDKDGFINIDE 60
           D+ ELRKVFDRFDANGDGKIS+SELDSVLK+ GSS+PYDELR VMEDLD+DKDGFINIDE
Sbjct: 29  DNAELRKVFDRFDANGDGKISLSELDSVLKSSGSSVPYDELRAVMEDLDTDKDGFINIDE 88

Query: 61  FAAFCKEPMASDEAGAAELRDAFDLYDQDHNGLISHSELHLVLNRLGITCTKEDCQRMIC 120
           FA FCKEP+AS+EAGA+ELRDAFDLYDQDHNGLIS SELHLVLNRLGITC+KEDCQRMI 
Sbjct: 89  FAVFCKEPIASNEAGASELRDAFDLYDQDHNGLISQSELHLVLNRLGITCSKEDCQRMIH 148

Query: 121 SVDSDGDGNVNFEEFRKMMTANSNS 146
           SVDSDGDGNVNFEEFRKMM+ NSNS
Sbjct: 149 SVDSDGDGNVNFEEFRKMMSNNSNS 173

[Close]

BLAST of MS002152 vs. ExPASy TrEMBL
Match: A0A0A0LPP8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G025020 PE=4 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 6.8e-62
Identity = 125/147 (85.03%), Postives = 135/147 (91.84%), Query Frame = 0


Query: 1   DSEELRKVFDRFDANGDGKISISELDSVLK--TLGSSIPYDELRRVMEDLDSDKDGFINI 60
           D EELRKVF+RFDANGDGKISISELD+VL   TL S+IP +ELR VM+DLDSDKDG+INI
Sbjct: 29  DDEELRKVFERFDANGDGKISISELDAVLTSLTLKSAIPLEELRSVMDDLDSDKDGYINI 88

Query: 61  DEFAAFCKEPMASDEAGAAELRDAFDLYDQDHNGLISHSELHLVLNRLGITCTKEDCQRM 120
           DEFAAFCK+PMASDEAGAAELRDAFDLYDQD NGLIS SELHLVLNRLGI+C+KEDCQ+M
Sbjct: 89  DEFAAFCKKPMASDEAGAAELRDAFDLYDQDRNGLISQSELHLVLNRLGISCSKEDCQKM 148

Query: 121 ICSVDSDGDGNVNFEEFRKMMTANSNS 146
           I SVDSDGDGNVNFEEFRKMMT NS S
Sbjct: 149 INSVDSDGDGNVNFEEFRKMMTDNSKS 175

[Close]

BLAST of MS002152 vs. ExPASy TrEMBL
Match: A0A6J1E0A3 (probable calcium-binding protein CML27 OS=Cucurbita moschata OX=3662 GN=LOC111429663 PE=4 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 1.2e-61
Identity = 121/145 (83.45%), Postives = 134/145 (92.41%), Query Frame = 0


Query: 1   DSEELRKVFDRFDANGDGKISISELDSVLKTLGSSIPYDELRRVMEDLDSDKDGFINIDE 60
           ++EELRKVFDRFD+NGDG+ISI+ELDSVLK+LGS+IPY+ELR VMEDLD DKDGFINIDE
Sbjct: 29  ENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGVMEDLDYDKDGFINIDE 88

Query: 61  FAAFCKEPMASDEAGAAELRDAFDLYDQDHNGLISHSELHLVLNRLGITCTKEDCQRMIC 120
           FAAFCKEPMASDEAG +ELRDAFDLYDQDHNGLISHSELHLVL RLGI  + EDCQRMI 
Sbjct: 89  FAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIH 148

Query: 121 SVDSDGDGNVNFEEFRKMMTANSNS 146
           +VDSDGDGNVNFEEFRKMM+  + S
Sbjct: 149 TVDSDGDGNVNFEEFRKMMSDTNKS 173

[Close]

BLAST of MS002152 vs. ExPASy TrEMBL
Match: A0A6J1JD54 (probable calcium-binding protein CML27 OS=Cucurbita maxima OX=3661 GN=LOC111485714 PE=4 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 2.6e-61
Identity = 120/145 (82.76%), Postives = 134/145 (92.41%), Query Frame = 0


Query: 1   DSEELRKVFDRFDANGDGKISISELDSVLKTLGSSIPYDELRRVMEDLDSDKDGFINIDE 60
           ++EELRKVFDRFD+NGDG+ISI+ELDSVLK+LGS+IPY+ELR +MEDLD DKDGFINIDE
Sbjct: 29  ENEELRKVFDRFDSNGDGRISITELDSVLKSLGSAIPYEELRGIMEDLDYDKDGFINIDE 88

Query: 61  FAAFCKEPMASDEAGAAELRDAFDLYDQDHNGLISHSELHLVLNRLGITCTKEDCQRMIC 120
           FAAFCKEPMASDEAG +ELRDAFDLYDQDHNGLISHSELHLVL RLGI  + EDCQRMI 
Sbjct: 89  FAAFCKEPMASDEAGLSELRDAFDLYDQDHNGLISHSELHLVLTRLGINYSIEDCQRMIH 148

Query: 121 SVDSDGDGNVNFEEFRKMMTANSNS 146
           +VDSDGDGNVNFEEFRKMM+  + S
Sbjct: 149 TVDSDGDGNVNFEEFRKMMSDANKS 173

[Close]

BLAST of MS002152 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 186.8 bits (473), Expect = 1.2e-47
Identity = 93/146 (63.70%), Postives = 117/146 (80.14%), Query Frame = 0


Query: 3   EELRKVFDRFDANGDGKISISELDSVLKTLGSSIPYDELRRVMEDLDSDKDGFINIDEFA 62
           EEL+KVFD+FD+NGDGKIS+ EL  V K +G+S    EL RV+E++D+D+DG+IN+DEF+
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 63  AFCKEPMASDEAGAAELRDAFDLYDQDHNGLISHSELHLVLNRLGITCTKEDCQRMICSV 122
             C+       + AAE+RDAFDLYDQD NGLIS SELH VLNRLG++C+ EDC RMI  V
Sbjct: 82  TLCR-----SSSSAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPV 141

Query: 123 DSDGDGNVNFEEFRKMMTANS--NSN 147
           D+DGDGNVNFEEF+KMMT++S  NSN
Sbjct: 142 DADGDGNVNFEEFQKMMTSSSLLNSN 162

[Close]

BLAST of MS002152 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 186.8 bits (473), Expect = 1.2e-47
Identity = 93/146 (63.70%), Postives = 117/146 (80.14%), Query Frame = 0


Query: 3   EELRKVFDRFDANGDGKISISELDSVLKTLGSSIPYDELRRVMEDLDSDKDGFINIDEFA 62
           EEL+KVFD+FD+NGDGKIS+ EL  V K +G+S    EL RV+E++D+D+DG+IN+DEF+
Sbjct: 22  EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 63  AFCKEPMASDEAGAAELRDAFDLYDQDHNGLISHSELHLVLNRLGITCTKEDCQRMICSV 122
             C+       + AAE+RDAFDLYDQD NGLIS SELH VLNRLG++C+ EDC RMI  V
Sbjct: 82  TLCR-----SSSSAAEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPV 141

Query: 123 DSDGDGNVNFEEFRKMMTANS--NSN 147
           D+DGDGNVNFEEF+KMMT++S  NSN
Sbjct: 142 DADGDGNVNFEEFQKMMTSSSLLNSN 162

[Close]

BLAST of MS002152 vs. TAIR 10
Match: AT1G73630.1 (EF hand calcium-binding protein family )

HSP 1 Score: 179.1 bits (453), Expect = 2.6e-45
Identity = 86/138 (62.32%), Postives = 111/138 (80.43%), Query Frame = 0


Query: 4   ELRKVFDRFDANGDGKISISELDSVLKTLGSSIPYDELRRVMEDLDSDKDGFINIDEFAA 63
           EL+KVFD+FDANGDGKIS+SEL +V K++G+S   +EL RV++++D D DGFIN +EFA 
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 64  FCKEPMASDEAGAAELRDAFDLYDQDHNGLISHSELHLVLNRLGITCTKEDCQRMICSVD 123
            C+       + A E+R+AFDLYDQ+ NGLIS SE+H VLNRLG+TC+ EDC RMI  VD
Sbjct: 80  ICR-----SSSSAVEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVD 139

Query: 124 SDGDGNVNFEEFRKMMTA 142
           +DGDGNVNFEEF+KMM++
Sbjct: 140 TDGDGNVNFEEFQKMMSS 152

[Close]

BLAST of MS002152 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 142.9 bits (359), Expect = 2.0e-34
Identity = 72/144 (50.00%), Postives = 99/144 (68.75%), Query Frame = 0


Query: 3   EELRKVFDRFDANGDGKISISELDSVLKTLGSSIPYDELRRVMEDLDSDKDGFINIDEFA 62
           E+++KVF RFD N DGKISI EL  V+  L  +   +E + +M++ D D +GFI++DEF 
Sbjct: 14  EDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFV 73

Query: 63  A-FCKEPMASDEAGAAELRDAFDLYDQDHNGLISHSELHLVLNRLGITCTKEDCQRMICS 122
           A F     +S+ +   +L++AFDLYD D NG IS +ELH V+  LG  C+ +DCQRMI  
Sbjct: 74  ALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQRMINK 133

Query: 123 VDSDGDGNVNFEEFRKMMTANSNS 146
           VDSDGDG V+FEEF+KMM  N ++
Sbjct: 134 VDSDGDGCVDFEEFKKMMMINGSA 157

[Close]

BLAST of MS002152 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 131.0 bits (328), Expect = 8.0e-31
Identity = 64/141 (45.39%), Postives = 95/141 (67.38%), Query Frame = 0


Query: 3   EELRKVFDRFDANGDGKISISELDSVLKTLGSSIPYDELRRVMEDLDSDKDGFINIDEFA 62
           ++++KVF RFD NGDGKIS+ EL  V++ L  +   +E   +M+  D D +GFI++DEF 
Sbjct: 16  DDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFV 75

Query: 63  AFCKEPM---ASDEAGAAELRDAFDLYDQDHNGLISHSELHLVLNRLGITCTKEDCQRMI 122
           A  +  +    ++    ++L++AF+LYD D NG IS  ELH V+  LG  C+ +DC++MI
Sbjct: 76  ALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMI 135

Query: 123 CSVDSDGDGNVNFEEFRKMMT 141
             VD DGDG VNF+EF+KMM+
Sbjct: 136 SKVDIDGDGCVNFDEFKKMMS 156

The following BLAST results are available for this feature:

BLAST of MS002152 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878467.1	4.8e-70	93.10	probable calcium-binding protein CML27 [Benincasa hispida]	[more]
XP_023529417.1	2.9e-67	89.66	probable calcium-binding protein CML27 [Cucurbita pepo subsp. pepo]	[more]
XP_022933917.1	2.9e-67	89.66	probable calcium-binding protein CML27 [Cucurbita moschata] >KAG7021647.1 putati...	[more]
KAG6587683.1	3.2e-66	88.97	putative calcium-binding protein CML27, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_023003784.1	4.2e-66	88.28	probable calcium-binding protein CML27 [Cucurbita maxima]	[more]

BLAST of MS002152 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LE22	1.7e-46	63.70	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ...	[more]
Q9C9U8	3.6e-44	62.32	Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 ...	[more]
Q9M7R0	1.1e-40	59.06	Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1	[more]
Q0DJV6	8.1e-36	53.74	Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9C8Y1	2.9e-33	50.00	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]

BLAST of MS002152 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F164	1.4e-67	89.66	probable calcium-binding protein CML27 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1KUB4	2.0e-66	88.28	probable calcium-binding protein CML27 OS=Cucurbita maxima OX=3661 GN=LOC1114972...	[more]
A0A0A0LPP8	6.8e-62	85.03	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G025020 PE=4 SV=1	[more]
A0A6J1E0A3	1.2e-61	83.45	probable calcium-binding protein CML27 OS=Cucurbita moschata OX=3662 GN=LOC11142...	[more]
A0A6J1JD54	2.6e-61	82.76	probable calcium-binding protein CML27 OS=Cucurbita maxima OX=3661 GN=LOC1114857...	[more]

BLAST of MS002152 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G18210.1	1.2e-47	63.70	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	1.2e-47	63.70	Calcium-binding EF-hand family protein	[more]
AT1G73630.1	2.6e-45	62.32	EF hand calcium-binding protein family	[more]
AT1G66400.1	2.0e-34	50.00	calmodulin like 23	[more]
AT5G37770.1	8.0e-31	45.39	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 78..106 e-value: 3.0E-4 score: 30.1 coord: 4..32 e-value: 1.3E-5 score: 34.6 coord: 40..68 e-value: 1.2E-4 score: 31.4 coord: 114..142 e-value: 1.2E-4 score: 31.4
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 78..139 e-value: 1.5E-12 score: 47.8 coord: 3..65 e-value: 5.0E-14 score: 52.5
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 74..109 score: 12.78389
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 36..71 score: 12.002819
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 110..145 score: 13.062845
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 1..35 score: 15.406057
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 78..140 e-value: 3.97819E-19 score: 73.7361
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 4..64 e-value: 1.49442E-15 score: 64.4913
None	No IPR available	GENE3D	1.10.238.10		coord: 2..70 e-value: 6.9E-21 score: 76.0
None	No IPR available	GENE3D	1.10.238.10		coord: 71..145 e-value: 5.8E-23 score: 83.0
None	No IPR available	PANTHER	PTHR10891:SF814	CALCIUM-BINDING PROTEIN CML26-RELATED	coord: 2..142
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 2..142
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 87..99
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 49..61
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 13..25
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 123..135
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 3..140

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS002152.1	MS002152.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding