MS002099 (gene) Bitter gourd (TR) v1

Overview


Name	MS002099
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Transcription initiation factor IIE subunit alpha
Location	scaffold30: 2353286 .. 2357806 (-)
RNA-Seq Expression	MS002099
Synteny	MS002099

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AATGATCTTGATCAATTGGGCAGGTTGGTGAAGCTGGCTGCGAGAGCTTTTTACGATGACATTACGACTAAGGGGGATAATCAGCCCAAGACTGGTCGGAGTGATAATAGAGGAATTGCGGTGGTTGTTCTTGATGCTCTCACGAGGTACTGCCCTTTCGCGTTTTGTTTCTTGGAAATCTTTATCGCCACCCAATATGTTGTTACTGAGAAATTGAAAGGAGGAGAGTTTCATTAGTGGACCGAGTTCTGGTTAGTTATGTTAGGATTATTGGACGGGGAACTCTTTTTTGATTTTGAGCTTATTGTACTTTTAACCGGGTTCTTGGTAGTTATATTTCCAGACTAAGCTCGCCACTGTTTTGTACTCCCAATTCTGGGTGTGCTGGAGTGGAACAGTTAAATCAATGGATATCATGGTTGTTTATTTATGTTTCTTGAAACTTATTAGTGTACGCTATTTCTCAAGTAAAGCTGAGTTGAAGAAAAGCAACACTGATTCATTTCATTCATATTGATGTAGCCTTCTCAATAGCTTAGTTGTATTTTTTTCTTCCTTCAAGGGGAGGTTATCATTTCATTTTTTCTTATTATTAGTTCATCTGCTAAAGAACAAGATCCTGTTTTTCAATCTAGATTGTGGATGGTCTGAGCAAAAAGCTGATTCAAAATGCCATTTTTAAGTTTCTTAATTTTTTACCATAAAGGATTTGGGAGATGATCCTGCCTAAGGTTTGTAATTTTATGTCCATTATATTTCTGTTCTTCAACTTGTTATGATGATGAAGCCATGCATGTATTATCTCCTTCTGCGGATCTTGTGAGCTGTTTTACTGCCACATTTTTTCTTTATTTATTTATAGCATTTGTTGTTGTATCCTGTCCTAGACGACAATGGGTTCGGGAAGAAGATTTGGCAAAGAACTTAAAACTACATTCGAAGCAATTGCGTCGAACCCTAAGGTTCCTAGAAGAGGAAAAACTGATTACCCGAGATCATAGAAGGGAGGTCAGTGCTGACTCTATCTATGCATCTTGTTCTTGTGCCTATTAGGTTAGTAGCTTGGAATATCTTAGTATGTTTTTCAGAATTTTAAATTGATTGTCTATAGCACCTAAGGTGCTTTTGTTGAATAGAGTTTGTGGTTTGATTTTGGTAAATTTAGTTAAAAAGAAATAAAAAAGTGTTTCAAGAAGCGGGAAGGCATGTGGAGAGCCTTGGGAAGTTAATGTTTTTGTTACCTCTTGGTGTATTTTGTTTAAATTTGTTTGTAATTTGTCATTTTTTCTGTCTGTTCTATTTGCCAATAGGAGAGCCTTTTTTTTTTTTTATATAATTTTTGGTGGATTGTTCATATGCTTAGTCCAAGAAAGAGAGAAAAAAAAAGTTGTTGGCCAGAGGAATGGCCAAATAGGCTTGCTGATGACAGGTAGTAACGGATTTTGACAAGAAAAAATAAATCTTGTTATCTTCAAGAAAATCTCTGAAGTTCTTAAATAAAAGGCTACTTTTTTGCAAGTTAGCCTTAGATCTGCATGAGTGCCACTAGCACTTTGGAGCATACTAATAGATATAGTTTACTTCATGCTGTATCCGTGATAGACTGCTGTTACTAGTGCTTATATTGCTTTACCTTCTTCCTTGTACTAGACATATGGACAACCCACTTTTCTCTCTTGTATGTGTTACGTCATTTTAGATCTTCACTAGTAGGGAGATTAGAAAATAAAAATAAAGGTTGGAATTAAGTAGAATGATGTTCACTCCCATCTTCTCACTTTTTTCTCTCTCATCTTACCTGTGATCTTTTTGCATTTCTTCATCTGAAGCTGATTAACATACGTGGTATGTAAACTTGTCTGGTATACTTTTTTTTGAACAAGATACGAACTTTTCATTGATATATGAAAAGGAACAAAATTGTTCAAAGATACAAACTCCCGAAGGAGTGATAGAGAAAGCAAGAAATAGAAAATAAAACATAAGACAAGACTACAAAGGAGAAATAAAGGCCTCCCAATTCATACAAATCATACTAGGGGAATACGAAGAGAACAGATCGGAAAGAGCACACCATTGAGAAGCTTTGAATTTGGCAATGTTGAAGCATTCCAACGGCAGGCGACAAGAATCTTCAAAAATCCTTTGATTTCTCTCAAACCATAATTCTGAGATGATAGCCTTAAATCCATTAAATCCAAAGTAGATTAGCTTTGGTGCCAACCCAACACCATGTAGAATCTGAAAAATATTATTAGCAGCCTCACGATCCCACACACATACTAATGGGAACATCTGGAGAAAAAGATTCCAACAAACAGCAGCATATGGGCAGAAAAAGAAGACATGATTTAAGCTTTCTTCATTTTCTGCACATAAAAAGCAAATGGATGGCTGTAAAGCCATCTTAGGAGACTTTCTCTGCAAGATATCTGCTGTATTAACTTGTCTGGTATACTTCATACAGGAAAATCTATTTGAATTCTGTTAAATATATTGCTGCTTAATTATTCATACTTTTTAATGTGGAGCAGCGAATCAATGAGTTATCTTAATATGATAACATTATTTATAGGCACGCTCTTCAACTTTTTGCTATTTAATTTTCCAGACAGCTAAGGGAGCAAAGATATTTAGTGCTGCAGTTGCTGCCACGGCCGATGGTCAACAGCCAGGGAAGGAGGGAGATGAAAAGATTAAGATGCACACACACTCTTATTGCTCGCTAGACTATGCTCAGGTTCTTCTTCTATAGGGTCACTACTGTAATAATAATAATTCTGATCAGTTACCTTATTAAAGCTAAACTTGGCTACAGATCTATGATGTGGTTAGATACAGAATGCACCGGATGAAGAAAAAACTGAAAGATGAATTAGAGGACAAGAACACAGTTCAAGAGTATATATGCCCCAACTGTTCGAGAAGGTTTTGATGATATGGTTTCTCTCTCTTTAAAAAATGTTATGTTCTTTCTATGTTTTTGTCATTTTAAGGAATCAAACATACACTTGTTATTGACTTCTTACCTGGAAGCAAGCTTGAAAGTTGTATGATATTGATTAGATACACTGCTTTGGATGCTCTGCGGTTGATCTCTCTTGAGGATGAATACTTTCACTGTGAAAATTGCAATGGAGAGCTGGTTGCAGAGAGTGACAAACTGGCGGCGGCTCAAGGAGGGGATGGAGATGATAATGCCAGGAAGCGACGGCATGAAAAATTAAAAGACATGCTTCAGAAGATGGAGGTTTTAATCTCTGGATTGTGTATTTTACTTTAATGGATTTTACATTTAGTATAATGACCCAAAAGAAATGTATTCTGAATCATCATCATTTTCATTTTTTTTTCCCTTTAAATCTTTGTATGTAATTTAATGAATTTCTATCTCAGGTATAGTTCTTTGGTGTAACGAGCTGATTTTTTTTCTTTAAACAAAGAACACTTGTCCTATAGAAAAATCATGATTCTCTTCTTCTCGGCTTCTGTCTATTCCAAAATTTATGGGGATGGCTGATCACAAAACTCTTTTACCTTTGTTAGCGTACAAGAACACTGACCTTGAAGCCATGTTTCTGAAAAAGTTTATGTGGGATTGTGTTTTTCTCCTTGAGTTTGTGTAGGGATGTTTCTATTGCTAAATGTTGACGAGTAAGTCAAATAATTGCAGGTCCAACTTAAACCTTTGATGGAACAACTCAGCAGAGTCAAAGATTTGCCTGTTCCGGAATTTGGAACTCTTTTAGCATGGGAAGCTCGTGCAAGTGCAGCTGCACGTGGGGCAAATGGTGATCTCAACGGTAATGATCCCTCGAAGTCATCGCAAGGTTTAGGATATGGGGGAACACCAATGCCTTTTGTTGGAGAGACCAAGGTATCCCTTCCTCATGCTTGTTATAGCTCTGGATCTTGAAAGGCACCACTCTAACCACTCATAAACTCATGAATCTCTTAGGTTGAAGTTGCCTTTTCTGGGGCTGAGGGCAAGGGAGTGGATGTTAAATCTGAAACTGAGAACACGGGACTGAAAGTTTTACCACCTTGGATGATCAAGAAAGGAATGAACCTCACAAAGGAACAACGTGGGGAGGTCAAAGAGGAGTCAAAGATGGATGCTGGTTCAGCTGCTCAGTTTTCTGATGATAAAAAGTCATCGGCCAATGACGATGATAAAACAAATTTTCAGGCTCGTTCAATAAGATTAGCTCTTTTCATTTTTTACTCATAACTCAAGTGCTTTTCTATCCAAAAATCTAAAATGGAAAGAGATTATATGATCTCATGTACTGGTGGTTGACTGTACTGTAGGATGAGTATGTTAAAGCTTATTATGCTGCTATACTGAAGAAGCAACAAGAACTTGAAGAATCTGCTAAGAGACCAGAGGAATTATCCAGTACAGAGGTTACTGAAAGTGTGTCTAATGCAAATTCCAGTCGCCAGGTGGGAATGAAAGCAAAACGTGAAGGGGATGAAGAAGATGATGATATTGAGTGGGAGGAGGCACCAATAGGGGGT

mRNA sequence

AATGATCTTGATCAATTGGGCAGGTTGGTGAAGCTGGCTGCGAGAGCTTTTTACGATGACATTACGACTAAGGGGGATAATCAGCCCAAGACTGGTCGGAGTGATAATAGAGGAATTGCGGTGGTTGTTCTTGATGCTCTCACGAGACGACAATGGGTTCGGGAAGAAGATTTGGCAAAGAACTTAAAACTACATTCGAAGCAATTGCGTCGAACCCTAAGGTTCCTAGAAGAGGAAAAACTGATTACCCGAGATCATAGAAGGGAGACAGCTAAGGGAGCAAAGATATTTAGTGCTGCAGTTGCTGCCACGGCCGATGGTCAACAGCCAGGGAAGGAGGGAGATGAAAAGATTAAGATGCACACACACTCTTATTGCTCGCTAGACTATGCTCAGATCTATGATGTGGTTAGATACAGAATGCACCGGATGAAGAAAAAACTGAAAGATGAATTAGAGGACAAGAACACAGTTCAAGAGTATATATGCCCCAACTGTTCGAGAAGATACACTGCTTTGGATGCTCTGCGGTTGATCTCTCTTGAGGATGAATACTTTCACTGTGAAAATTGCAATGGAGAGCTGGTTGCAGAGAGTGACAAACTGGCGGCGGCTCAAGGAGGGGATGGAGATGATAATGCCAGGAAGCGACGGCATGAAAAATTAAAAGACATGCTTCAGAAGATGGAGGTCCAACTTAAACCTTTGATGGAACAACTCAGCAGAGTCAAAGATTTGCCTGTTCCGGAATTTGGAACTCTTTTAGCATGGGAAGCTCGTGCAAGTGCAGCTGCACGTGGGGCAAATGGTGATCTCAACGGTAATGATCCCTCGAAGTCATCGCAAGGTTTAGGATATGGGGGAACACCAATGCCTTTTGTTGGAGAGACCAAGGTTGAAGTTGCCTTTTCTGGGGCTGAGGGCAAGGGAGTGGATGTTAAATCTGAAACTGAGAACACGGGACTGAAAGTTTTACCACCTTGGATGATCAAGAAAGGAATGAACCTCACAAAGGAACAACGTGGGGAGGTCAAAGAGGAGTCAAAGATGGATGCTGGTTCAGCTGCTCAGTTTTCTGATGATAAAAAGTCATCGGCCAATGACGATGATAAAACAAATTTTCAGGATGAGTATGTTAAAGCTTATTATGCTGCTATACTGAAGAAGCAACAAGAACTTGAAGAATCTGCTAAGAGACCAGAGGAATTATCCAGTACAGAGGTTACTGAAAGTGTGTCTAATGCAAATTCCAGTCGCCAGGTGGGAATGAAAGCAAAACGTGAAGGGGATGAAGAAGATGATGATATTGAGTGGGAGGAGGCACCAATAGGGGGT

Coding sequence (CDS)

AATGATCTTGATCAATTGGGCAGGTTGGTGAAGCTGGCTGCGAGAGCTTTTTACGATGACATTACGACTAAGGGGGATAATCAGCCCAAGACTGGTCGGAGTGATAATAGAGGAATTGCGGTGGTTGTTCTTGATGCTCTCACGAGACGACAATGGGTTCGGGAAGAAGATTTGGCAAAGAACTTAAAACTACATTCGAAGCAATTGCGTCGAACCCTAAGGTTCCTAGAAGAGGAAAAACTGATTACCCGAGATCATAGAAGGGAGACAGCTAAGGGAGCAAAGATATTTAGTGCTGCAGTTGCTGCCACGGCCGATGGTCAACAGCCAGGGAAGGAGGGAGATGAAAAGATTAAGATGCACACACACTCTTATTGCTCGCTAGACTATGCTCAGATCTATGATGTGGTTAGATACAGAATGCACCGGATGAAGAAAAAACTGAAAGATGAATTAGAGGACAAGAACACAGTTCAAGAGTATATATGCCCCAACTGTTCGAGAAGATACACTGCTTTGGATGCTCTGCGGTTGATCTCTCTTGAGGATGAATACTTTCACTGTGAAAATTGCAATGGAGAGCTGGTTGCAGAGAGTGACAAACTGGCGGCGGCTCAAGGAGGGGATGGAGATGATAATGCCAGGAAGCGACGGCATGAAAAATTAAAAGACATGCTTCAGAAGATGGAGGTCCAACTTAAACCTTTGATGGAACAACTCAGCAGAGTCAAAGATTTGCCTGTTCCGGAATTTGGAACTCTTTTAGCATGGGAAGCTCGTGCAAGTGCAGCTGCACGTGGGGCAAATGGTGATCTCAACGGTAATGATCCCTCGAAGTCATCGCAAGGTTTAGGATATGGGGGAACACCAATGCCTTTTGTTGGAGAGACCAAGGTTGAAGTTGCCTTTTCTGGGGCTGAGGGCAAGGGAGTGGATGTTAAATCTGAAACTGAGAACACGGGACTGAAAGTTTTACCACCTTGGATGATCAAGAAAGGAATGAACCTCACAAAGGAACAACGTGGGGAGGTCAAAGAGGAGTCAAAGATGGATGCTGGTTCAGCTGCTCAGTTTTCTGATGATAAAAAGTCATCGGCCAATGACGATGATAAAACAAATTTTCAGGATGAGTATGTTAAAGCTTATTATGCTGCTATACTGAAGAAGCAACAAGAACTTGAAGAATCTGCTAAGAGACCAGAGGAATTATCCAGTACAGAGGTTACTGAAAGTGTGTCTAATGCAAATTCCAGTCGCCAGGTGGGAATGAAAGCAAAACGTGAAGGGGATGAAGAAGATGATGATATTGAGTGGGAGGAGGCACCAATAGGGGGT

Protein sequence

NDLDQLGRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQQPGKEGDEKIKMHTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLEDEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSRVKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVAFSGAEGKGVDVKSETENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSAAQFSDDKKSSANDDDKTNFQDEYVKAYYAAILKKQQELEESAKRPEELSSTEVTESVSNANSSRQVGMKAKREGDEEDDDIEWEEAPIGG

Homology

[Close]

BLAST of MS002099 vs. NCBI nr
Match: XP_022134956.1 (general transcription factor IIE subunit 1 [Momordica charantia])

HSP 1 Score: 849.4 bits (2193), Expect = 1.4e-242
Identity = 438/443 (98.87%), Postives = 440/443 (99.32%), Query Frame = 0


Query: 3   LDQLGRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62
           ++   RLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL
Sbjct: 3   IEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62

Query: 63  KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQQPGKEGDEKIKMHT 122
           KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQQPGKEGDEKIKMHT
Sbjct: 63  KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQQPGKEGDEKIKMHT 122

Query: 123 HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE 182
           HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE
Sbjct: 123 HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE 182

Query: 183 DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR 242
           DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR
Sbjct: 183 DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR 242

Query: 243 VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA 302
           VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA
Sbjct: 243 VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA 302

Query: 303 FSGAEGKGVDVKSETENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSAAQFSDDK 362
           FSGAEGKGVDVKSETENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSAAQFSDDK
Sbjct: 303 FSGAEGKGVDVKSETENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSAAQFSDDK 362

Query: 363 KSSANDDDKTNFQDEYVKAYYAAILKKQQELEESAKRPEELSSTEVTESVSNANSSRQVG 422
           KSSANDDDKTNFQDEYVKAYYAAILKKQQELEESAKRPEELSSTEVTESVSNANSSRQVG
Sbjct: 363 KSSANDDDKTNFQDEYVKAYYAAILKKQQELEESAKRPEELSSTEVTESVSNANSSRQVG 422

Query: 423 MKAKREGDEEDDDIEWEEAPIGG 446
           MKAKREGDEEDDDIEWEEAPIGG
Sbjct: 423 MKAKREGDEEDDDIEWEEAPIGG 445

[Close]

BLAST of MS002099 vs. NCBI nr
Match: XP_038880289.1 (general transcription factor IIE subunit 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 808.9 bits (2088), Expect = 2.2e-230
Identity = 422/444 (95.05%), Postives = 430/444 (96.85%), Query Frame = 0


Query: 3   LDQLGRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62
           ++   RLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL
Sbjct: 3   IEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62

Query: 63  KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQQPGKEGDEKIKMHT 122
           KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQ P KEGDEKIKMHT
Sbjct: 63  KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKMHT 122

Query: 123 HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE 182
           HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS E
Sbjct: 123 HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISFE 182

Query: 183 DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR 242
           DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR
Sbjct: 183 DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR 242

Query: 243 VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA 302
           VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKS+QGLGYGGTPMPFVGETKVEVA
Sbjct: 243 VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSAQGLGYGGTPMPFVGETKVEVA 302

Query: 303 FSGAEGKGVDVKSETENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSA-AQFSDD 362
           FSGAEGKGVDVKSE+ENTGLKVLPPWMIKKGMNLTKEQRGEVK+ESKMDAGSA AQFSDD
Sbjct: 303 FSGAEGKGVDVKSESENTGLKVLPPWMIKKGMNLTKEQRGEVKDESKMDAGSASAQFSDD 362

Query: 363 KKSSANDDDKTNFQDEYVKAYYAAILKKQQELEESAKRPEELSSTEVTESVSNANSSRQV 422
           KKSSANDDDKTN QDEYVKAYYAAILKKQQELEE+AK  EEL STEVTE+V+N NSSRQV
Sbjct: 363 KKSSANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKGQEELPSTEVTENVANRNSSRQV 422

Query: 423 GMKAKREGDEEDDDIEWEEAPIGG 446
           GMKAKRE DEE DDIEWEEAPIGG
Sbjct: 423 GMKAKREEDEE-DDIEWEEAPIGG 445

[Close]

BLAST of MS002099 vs. NCBI nr
Match: XP_038880290.1 (general transcription factor IIE subunit 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 807.0 bits (2083), Expect = 8.2e-230
Identity = 421/443 (95.03%), Postives = 429/443 (96.84%), Query Frame = 0


Query: 3   LDQLGRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62
           ++   RLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL
Sbjct: 3   IEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62

Query: 63  KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQQPGKEGDEKIKMHT 122
           KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQ P KEGDEKIKMHT
Sbjct: 63  KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHPVKEGDEKIKMHT 122

Query: 123 HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE 182
           HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS E
Sbjct: 123 HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISFE 182

Query: 183 DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR 242
           DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR
Sbjct: 183 DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR 242

Query: 243 VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA 302
           VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKS+QGLGYGGTPMPFVGETKVEVA
Sbjct: 243 VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSAQGLGYGGTPMPFVGETKVEVA 302

Query: 303 FSGAEGKGVDVKSETENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSA-AQFSDD 362
           FSGAEGKGVDVKSE+ENTGLKVLPPWMIKKGMNLTKEQRGEVK+ESKMDAGSA AQFSDD
Sbjct: 303 FSGAEGKGVDVKSESENTGLKVLPPWMIKKGMNLTKEQRGEVKDESKMDAGSASAQFSDD 362

Query: 363 KKSSANDDDKTNFQDEYVKAYYAAILKKQQELEESAKRPEELSSTEVTESVSNANSSRQV 422
           KKSSANDDDKTN QDEYVKAYYAAILKKQQELEE+AK  EEL STEVTE+V+N NSSRQV
Sbjct: 363 KKSSANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKGQEELPSTEVTENVANRNSSRQV 422

Query: 423 GMKAKREGDEEDDDIEWEEAPIG 445
           GMKAKRE DEE DDIEWEEAPIG
Sbjct: 423 GMKAKREEDEE-DDIEWEEAPIG 444

[Close]

BLAST of MS002099 vs. NCBI nr
Match: XP_022922068.1 (general transcription factor IIE subunit 1-like [Cucurbita moschata] >KAG6589563.1 General transcription factor IIE subunit 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023252.1 General transcription factor IIE subunit 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 804.3 bits (2076), Expect = 5.3e-229
Identity = 415/444 (93.47%), Postives = 430/444 (96.85%), Query Frame = 0


Query: 3   LDQLGRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62
           ++   RLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL
Sbjct: 3   IEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62

Query: 63  KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQQPGKEGDEKIKMHT 122
           KLHSKQLRRTLRFLEEEKL+TRDHRRETAKGAKIFSAA+AATADGQ PGKEGDEKIKMHT
Sbjct: 63  KLHSKQLRRTLRFLEEEKLVTRDHRRETAKGAKIFSAAIAATADGQHPGKEGDEKIKMHT 122

Query: 123 HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE 182
           HSYCSLDYAQ+YDVVRYR+HRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE
Sbjct: 123 HSYCSLDYAQVYDVVRYRLHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE 182

Query: 183 DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR 242
           DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR
Sbjct: 183 DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR 242

Query: 243 VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA 302
           VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA
Sbjct: 243 VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA 302

Query: 303 FSGAEGKGVDVKSETENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSA-AQFSDD 362
           FSGAEGKGV+VKSE+ENT LKVLPPWMIK+GMNLTKEQRGEVK +SKMDAGSA AQFSDD
Sbjct: 303 FSGAEGKGVNVKSESENTALKVLPPWMIKEGMNLTKEQRGEVKGDSKMDAGSASAQFSDD 362

Query: 363 KKSSANDDDKTNFQDEYVKAYYAAILKKQQELEESAKRPEELSSTEVTESVSNANSSRQV 422
           KKSSANDDDKTN QDEYVKAYYAAILKKQQELEE+AK  +ELSSTEVTE++S  NSSRQV
Sbjct: 363 KKSSANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKAQKELSSTEVTENLSYTNSSRQV 422

Query: 423 GMKAKREGDEEDDDIEWEEAPIGG 446
           GMKAKRE DEE+DDIEWEEAPI G
Sbjct: 423 GMKAKREEDEEEDDIEWEEAPIEG 446

[Close]

BLAST of MS002099 vs. NCBI nr
Match: XP_023515738.1 (general transcription factor IIE subunit 1-like [Cucurbita pepo subsp. pepo] >XP_023515739.1 general transcription factor IIE subunit 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 801.2 bits (2068), Expect = 4.5e-228
Identity = 413/444 (93.02%), Postives = 430/444 (96.85%), Query Frame = 0


Query: 3   LDQLGRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62
           ++   RLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL
Sbjct: 3   IEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62

Query: 63  KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQQPGKEGDEKIKMHT 122
           KLHSKQLRRTLRFLEEEKL+TRDHRRETAKGAKIFSAA+AATADGQ PGKEGDEKIKMHT
Sbjct: 63  KLHSKQLRRTLRFLEEEKLVTRDHRRETAKGAKIFSAAIAATADGQHPGKEGDEKIKMHT 122

Query: 123 HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE 182
           HSYCSLDYAQ+YDVVRYR+HRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE
Sbjct: 123 HSYCSLDYAQVYDVVRYRLHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE 182

Query: 183 DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR 242
           DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR
Sbjct: 183 DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR 242

Query: 243 VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA 302
           VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA
Sbjct: 243 VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA 302

Query: 303 FSGAEGKGVDVKSETENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSAA-QFSDD 362
           FSGAEGKGV+VKSE+ENT LKVLPPWMIK+GMNLTKEQRGEVK E+KMDAGSA+ QFSDD
Sbjct: 303 FSGAEGKGVNVKSESENTTLKVLPPWMIKEGMNLTKEQRGEVKGEAKMDAGSASTQFSDD 362

Query: 363 KKSSANDDDKTNFQDEYVKAYYAAILKKQQELEESAKRPEELSSTEVTESVSNANSSRQV 422
           KKSSANDDDKTN QDEYVKAYYAAILKKQQELEE+AK  +ELSSTEVTE++S  NSSRQV
Sbjct: 363 KKSSANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKGQKELSSTEVTENLSYTNSSRQV 422

Query: 423 GMKAKREGDEEDDDIEWEEAPIGG 446
           GMKAKRE D+E+DDIEWEEAPI G
Sbjct: 423 GMKAKREEDDEEDDIEWEEAPIDG 446

[Close]

BLAST of MS002099 vs. ExPASy Swiss-Prot
Match: Q557M8 (General transcription factor IIE subunit 1 OS=Dictyostelium discoideum OX=44689 GN=gtf2e1-1 PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.6e-18
Identity = 100/393 (25.45%), Postives = 167/393 (42.49%), Query Frame = 0


Query: 6   LGRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNLKLH 65
           L  LVK+  RAFY D                   AV++   L  ++ +++EDLA  L++ 
Sbjct: 11  LDDLVKMVIRAFYPD-----------------EYAVIIDGLLREKKRIKDEDLALRLRIQ 70

Query: 66  SKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQQPGKEGDEKIKMHTHSY 125
            K +R+ L  L+ + ++     +  AKG                P + G       TH  
Sbjct: 71  QKYVRKILMDLKGDSMVKSSDVKVEAKG----------------PNERGS------THLL 130

Query: 126 CSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLEDEY 185
             +DY  I D+V+Y+++  +KK++     K  VQ Y C  C + YTALD  +L++++   
Sbjct: 131 WYIDYKHIIDIVKYKLYMFRKKMESVKVQKIDVQTYKCQTCHKVYTALDIPKLLNMDTGA 190

Query: 186 FHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSRVKD 245
             CE C+GEL  E +  +  Q           +H+   D+      QL+ ++EQL + + 
Sbjct: 191 LACEICDGELEEELNNESLTQ---------TAKHQ--SDLFS----QLRKIIEQLKKTEG 250

Query: 246 LPVPEFGTLLAWEARASAAARGANGDLNGND-----PSKSSQGLGYGGTPMPFVGETKVE 305
             +P F   LA      +A +G +  +N N      P  S+  +  G      +  T   
Sbjct: 251 HNIPLFARDLA----DLSADQGPSYTINTNSSLGMGPKPSAFPVAQGAATSHHIDPTNEN 310

Query: 306 VAF-------SGAEGKGVDVKSETENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAG 365
           + F        G E     VK E + TGL  LPPW++    N  K +   VK  S ++  
Sbjct: 311 IEFHVDILDTDGIEINKAVVKKENKKTGLASLPPWLLPS--NSFKNR--NVKSNSILNNN 341

Query: 366 SAAQFSDDKKSSANDDD---KTNFQDEYVKAYY 384
              Q S +++ +A  +      +F   Y+K +Y
Sbjct: 371 QQTQTSTNEQPTAVKEQIKIDQDFYINYIKTHY 341

[Close]

BLAST of MS002099 vs. ExPASy Swiss-Prot
Match: P36100 (Transcription initiation factor IIE subunit alpha OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TFA1 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 2.3e-09
Identity = 91/437 (20.82%), Postives = 167/437 (38.22%), Query Frame = 0


Query: 38  GIAVVVLDALTRRQWVREEDLAKNLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIF 97
           G  V+VLDA+     + E+DL + L ++  +L   +  L  ++LI+   +RE        
Sbjct: 23  GSFVLVLDAILFHSVLAEDDLKQLLSINKTELGPLIARLRSDRLISIHKQRE-------- 82

Query: 98  SAAVAATADGQQPGKEGDEKIKMHTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNT 157
                       P  +  E++      Y  + Y    D +++++H++ ++LKD+L+  + 
Sbjct: 83  ----------YPPNSKSVERV------YYYVKYPHAIDAIKWKVHQVVQRLKDDLDKNSE 142

Query: 158 VQEYICPNCSRRYTALDALRLISLEDEYFHCENCNGELVAESDKLAAAQGGDGDDNARKR 217
              Y+CP C  +YT L+A++L++ +   F C  C+  LV              +D++ K+
Sbjct: 143 PNGYMCPICLTKYTQLEAVQLLNFDRTEFLCSLCDEPLV--------------EDDSGKK 202

Query: 218 RHEKLKDMLQKMEVQLKPLMEQLSRVKDLPVPE--FGTLLA------------------- 277
             EK +D L ++  Q++P+++ L ++ D  + E  F   LA                   
Sbjct: 203 NKEK-QDKLNRLMDQIQPIIDSLKKIDDSRIEENTFEIALARLIPPQNQSHAAYTYNPKK 262

Query: 278 ----WEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVAFSGAEGKGVD 337
               +    SA      G   GND S+ +      G          +  A      + + 
Sbjct: 263 GSTMFRPGDSAPLPNLMGTALGNDSSRRA------GANSQATLHINITTASDEVAQRELQ 322

Query: 338 VKSETENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSAAQFSDDKKSSANDDDKT 397
            +   E      +P W   K   + K   G +  E + D    A   D          +T
Sbjct: 323 ERQAEEKRKQNAVPEW--HKQSTIGKTALGRLDNEEEFDPVVTASAMDSINPDNEPAQET 382

Query: 398 NFQ---------------DEYVKAYYAAILKKQQELEESAKRPEELSSTEVTESVSNANS 435
           ++Q               ++ +  YYAA+ KKQ +L +  +  EE    E  E       
Sbjct: 383 SYQNNRTLTEQEMEERENEKTLNDYYAALAKKQAKLNKEEEEEEEEEEDEEEEEEEEMED 412

[Close]

BLAST of MS002099 vs. ExPASy Swiss-Prot
Match: P29083 (General transcription factor IIE subunit 1 OS=Homo sapiens OX=9606 GN=GTF2E1 PE=1 SV=2)

HSP 1 Score: 64.7 bits (156), Expect = 3.0e-09
Identity = 53/205 (25.85%), Postives = 92/205 (44.88%), Query Frame = 0


Query: 42  VVLDALTRRQWVREEDLAKNLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAV 101
           + LD L R   V+EED+ + LK   KQLR  L  L+ +K I    R ETA          
Sbjct: 32  LALDILIRNSCVKEEDMLELLKFDRKQLRSVLNNLKGDKFIKCRMRVETA---------- 91

Query: 102 AATADGQQPGKEGDEKIKMHTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEY 161
              ADG           K   H+Y  ++Y  + +VV+Y++  M+++++ +  D      +
Sbjct: 92  ---ADG-----------KTTRHNYYFINYRTLVNVVKYKLDHMRRRIETDERDSTNRASF 151

Query: 162 ICPNCSRRYTALDALRLISLEDEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEK 221
            CP CS  +T L+A +L       F C  C+ E+             + D++A  ++   
Sbjct: 152 KCPVCSSTFTDLEANQLFDPMTGTFRCTFCHTEV-------------EEDESAMPKK--D 197

Query: 222 LKDMLQKMEVQLKPLMEQLSRVKDL 247
            + +L +   Q++P+   L   +D+
Sbjct: 212 ARTLLARFNEQIEPIYALLRETEDV 197

[Close]

BLAST of MS002099 vs. ExPASy Swiss-Prot
Match: Q5R8H5 (General transcription factor IIE subunit 1 OS=Pongo abelii OX=9601 GN=GTF2E1 PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 4.0e-09
Identity = 53/205 (25.85%), Postives = 92/205 (44.88%), Query Frame = 0


Query: 42  VVLDALTRRQWVREEDLAKNLKLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAV 101
           + LD L R   V+EED+ + LK   KQLR  L  L+ +K I    R ETA          
Sbjct: 32  LALDILIRNPCVKEEDMLELLKFDRKQLRSVLNNLKGDKFIKCRMRVETA---------- 91

Query: 102 AATADGQQPGKEGDEKIKMHTHSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEY 161
              ADG           K   H+Y  ++Y  + +VV+Y++  M+++++ +  D      +
Sbjct: 92  ---ADG-----------KTTRHNYYFINYRTLVNVVKYKLDHMRRRIETDERDSTNRASF 151

Query: 162 ICPNCSRRYTALDALRLISLEDEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEK 221
            CP CS  +T L+A +L       F C  C+ E+             + D++A  ++   
Sbjct: 152 KCPVCSSTFTDLEANQLFDPMTGTFRCTFCHTEV-------------EEDESAMPKK--D 197

Query: 222 LKDMLQKMEVQLKPLMEQLSRVKDL 247
            + +L +   Q++P+   L   +D+
Sbjct: 212 ARTLLARFNEQIEPIYALLRETEDV 197

[Close]

BLAST of MS002099 vs. ExPASy Swiss-Prot
Match: Q9P3W1 (Transcription initiation factor IIE subunit alpha OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tfa1 PE=1 SV=3)

HSP 1 Score: 62.8 bits (151), Expect = 1.2e-08
Identity = 104/443 (23.48%), Postives = 177/443 (39.95%), Query Frame = 0


Query: 8   RLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNLKLHSK 67
           RL+K+  RAFY+                     ++ +DA+ R   + +E  A  + +  K
Sbjct: 10  RLIKMIMRAFYET------------------RHIIFMDAILRHSALTDEQTALLMGIPIK 69

Query: 68  QLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQQPGKEGDEKIKMHTHSYCS 127
           + R     L E++L+    R E                      KEG ++ + HT +Y  
Sbjct: 70  ECRFIAGKLREDRLLAIQSRTEM---------------------KEGQQR-QYHT-TYFY 129

Query: 128 LDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLEDEYFH 187
           +D+    D +++RMH++ K ++D + +    + Y+CP C++++++LD L L++ E   F 
Sbjct: 130 IDFCSTIDSIKWRMHQLVKTVEDRMRNDFDSKGYVCPFCNKKFSSLDVLSLVTNEGT-FA 189

Query: 188 CENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSRVKDLP 247
           C  C  EL             D +++A     +K    L K+  Q+  +++ L RV ++ 
Sbjct: 190 CNVCGTEL------------KDDEESAEMMSSQK---RLGKLMGQVNGIIDALKRVDEIV 249

Query: 248 VPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVAFSGAE 307
           VP+     A E     +    N  L+  + SKS+  +              + V FS   
Sbjct: 250 VPQNNFQSALEHAVPVSLDTQN--LSQQNLSKSNSDVRLS------TSSPSITVDFSA-- 309

Query: 308 GKGVDVKSETE-----NTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSAAQFSDDK 367
            K  D K E            +LP W      + T    G +      DA  A      +
Sbjct: 310 DKETDEKRERNCDKQVKAAQNILPEW------HATSTISGSITRAGAKDA--ALHSFRTE 369

Query: 368 KSSANDDDKTNFQDE--YVKAYYAAILKKQQEL-----EESAKRPEELSSTEVTESVSNA 427
             +   D KT+   E   + AYYA +  KQ+E       E+    E+    +VT + S  
Sbjct: 370 TVNEVQDTKTDITSEKSALDAYYATLRAKQKEESEFMDSENVDDEEDDDFLDVTTATSLQ 377

Query: 428 NSSRQVG-MKAKREGDEEDDDIE 438
           N S   G +K K E    D DI+
Sbjct: 430 NKSTDYGSVKRKTENLNSDSDIQ 377

[Close]

BLAST of MS002099 vs. ExPASy TrEMBL
Match: A0A6J1BZ86 (Transcription initiation factor IIE subunit alpha OS=Momordica charantia OX=3673 GN=LOC111007077 PE=3 SV=1)

HSP 1 Score: 849.4 bits (2193), Expect = 7.0e-243
Identity = 438/443 (98.87%), Postives = 440/443 (99.32%), Query Frame = 0


Query: 3   LDQLGRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62
           ++   RLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL
Sbjct: 3   IEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62

Query: 63  KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQQPGKEGDEKIKMHT 122
           KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQQPGKEGDEKIKMHT
Sbjct: 63  KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQQPGKEGDEKIKMHT 122

Query: 123 HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE 182
           HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE
Sbjct: 123 HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE 182

Query: 183 DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR 242
           DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR
Sbjct: 183 DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR 242

Query: 243 VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA 302
           VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA
Sbjct: 243 VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA 302

Query: 303 FSGAEGKGVDVKSETENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSAAQFSDDK 362
           FSGAEGKGVDVKSETENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSAAQFSDDK
Sbjct: 303 FSGAEGKGVDVKSETENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSAAQFSDDK 362

Query: 363 KSSANDDDKTNFQDEYVKAYYAAILKKQQELEESAKRPEELSSTEVTESVSNANSSRQVG 422
           KSSANDDDKTNFQDEYVKAYYAAILKKQQELEESAKRPEELSSTEVTESVSNANSSRQVG
Sbjct: 363 KSSANDDDKTNFQDEYVKAYYAAILKKQQELEESAKRPEELSSTEVTESVSNANSSRQVG 422

Query: 423 MKAKREGDEEDDDIEWEEAPIGG 446
           MKAKREGDEEDDDIEWEEAPIGG
Sbjct: 423 MKAKREGDEEDDDIEWEEAPIGG 445

[Close]

BLAST of MS002099 vs. ExPASy TrEMBL
Match: A0A6J1E5J2 (Transcription initiation factor IIE subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111430131 PE=3 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.6e-229
Identity = 415/444 (93.47%), Postives = 430/444 (96.85%), Query Frame = 0


Query: 3   LDQLGRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62
           ++   RLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL
Sbjct: 3   IEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62

Query: 63  KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQQPGKEGDEKIKMHT 122
           KLHSKQLRRTLRFLEEEKL+TRDHRRETAKGAKIFSAA+AATADGQ PGKEGDEKIKMHT
Sbjct: 63  KLHSKQLRRTLRFLEEEKLVTRDHRRETAKGAKIFSAAIAATADGQHPGKEGDEKIKMHT 122

Query: 123 HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE 182
           HSYCSLDYAQ+YDVVRYR+HRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE
Sbjct: 123 HSYCSLDYAQVYDVVRYRLHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE 182

Query: 183 DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR 242
           DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR
Sbjct: 183 DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR 242

Query: 243 VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA 302
           VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA
Sbjct: 243 VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA 302

Query: 303 FSGAEGKGVDVKSETENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSA-AQFSDD 362
           FSGAEGKGV+VKSE+ENT LKVLPPWMIK+GMNLTKEQRGEVK +SKMDAGSA AQFSDD
Sbjct: 303 FSGAEGKGVNVKSESENTALKVLPPWMIKEGMNLTKEQRGEVKGDSKMDAGSASAQFSDD 362

Query: 363 KKSSANDDDKTNFQDEYVKAYYAAILKKQQELEESAKRPEELSSTEVTESVSNANSSRQV 422
           KKSSANDDDKTN QDEYVKAYYAAILKKQQELEE+AK  +ELSSTEVTE++S  NSSRQV
Sbjct: 363 KKSSANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKAQKELSSTEVTENLSYTNSSRQV 422

Query: 423 GMKAKREGDEEDDDIEWEEAPIGG 446
           GMKAKRE DEE+DDIEWEEAPI G
Sbjct: 423 GMKAKREEDEEEDDIEWEEAPIEG 446

[Close]

BLAST of MS002099 vs. ExPASy TrEMBL
Match: A0A6J1JA07 (Transcription initiation factor IIE subunit alpha OS=Cucurbita maxima OX=3661 GN=LOC111484895 PE=3 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 1.4e-227
Identity = 412/444 (92.79%), Postives = 428/444 (96.40%), Query Frame = 0


Query: 3   LDQLGRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62
           ++   RLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL
Sbjct: 3   IEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62

Query: 63  KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQQPGKEGDEKIKMHT 122
           KLHSKQLRRTLRFLEEEKL+TRDHRRETAKGAKIFSAAVAATADGQ PGKEGDEKIKMHT
Sbjct: 63  KLHSKQLRRTLRFLEEEKLVTRDHRRETAKGAKIFSAAVAATADGQHPGKEGDEKIKMHT 122

Query: 123 HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE 182
           HSYCSLDYAQ+YDVVRYR+HRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE
Sbjct: 123 HSYCSLDYAQVYDVVRYRLHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE 182

Query: 183 DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR 242
           DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR
Sbjct: 183 DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR 242

Query: 243 VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA 302
           VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA
Sbjct: 243 VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA 302

Query: 303 FSGAEGKGVDVKSETENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSA-AQFSDD 362
           FSGAE KGV+VKSE+ENT LKVLPPWMIK+GMNLTKEQRGEVK ESKMDAGSA AQFSDD
Sbjct: 303 FSGAESKGVNVKSESENTALKVLPPWMIKEGMNLTKEQRGEVKGESKMDAGSASAQFSDD 362

Query: 363 KKSSANDDDKTNFQDEYVKAYYAAILKKQQELEESAKRPEELSSTEVTESVSNANSSRQV 422
           KKSSANDDDKTN QDEYVKAYYAAILKKQQELEE+AK  +ELS TE+T+++S  N+SRQV
Sbjct: 363 KKSSANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKGQKELSRTEITDNLSYTNASRQV 422

Query: 423 GMKAKREGDEEDDDIEWEEAPIGG 446
           GMKAKRE DEE+DDIEWEEAPI G
Sbjct: 423 GMKAKREEDEEEDDIEWEEAPIEG 446

[Close]

BLAST of MS002099 vs. ExPASy TrEMBL
Match: A0A5D3DYI4 (Transcription initiation factor IIE subunit alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00230 PE=3 SV=1)

HSP 1 Score: 788.9 bits (2036), Expect = 1.1e-224
Identity = 410/444 (92.34%), Postives = 424/444 (95.50%), Query Frame = 0


Query: 3   LDQLGRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62
           ++   RLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL
Sbjct: 3   IEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62

Query: 63  KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQQPGKEGDEKIKMHT 122
           KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQ   KEGDEKIKMHT
Sbjct: 63  KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHQTKEGDEKIKMHT 122

Query: 123 HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE 182
           HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS E
Sbjct: 123 HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISFE 182

Query: 183 DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR 242
           DEYFHCENCNGELVAESDKLAA QGGDGDDNARKRRHEKLKDMLQKME QLKPL+EQL+R
Sbjct: 183 DEYFHCENCNGELVAESDKLAAVQGGDGDDNARKRRHEKLKDMLQKMEAQLKPLVEQLTR 242

Query: 243 VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA 302
           VKDLPVPEFGTLLAWEARASAAARG NGDLNGNDPSKSSQ  GYGGTPMPFVGETKVEVA
Sbjct: 243 VKDLPVPEFGTLLAWEARASAAARGVNGDLNGNDPSKSSQ--GYGGTPMPFVGETKVEVA 302

Query: 303 FSGAEGKGVDVKSETENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSA-AQFSDD 362
           FSGA+GKGVDVKSE++NTGLKVLPPWMIK+GMNLTKEQRGEVK+ESK DAGSA AQFSDD
Sbjct: 303 FSGADGKGVDVKSESKNTGLKVLPPWMIKQGMNLTKEQRGEVKDESKTDAGSASAQFSDD 362

Query: 363 KKSSANDDDKTNFQDEYVKAYYAAILKKQQELEESAKRPEELSSTEVTESVSNANSSRQV 422
           KKS ANDDDKTN QDEYVKAYYAAILKKQQELEE+AK  +ELSSTEVTESV+N NSSRQV
Sbjct: 363 KKSLANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKGQQELSSTEVTESVANTNSSRQV 422

Query: 423 GMKAKREGDEEDDDIEWEEAPIGG 446
           GMKAKRE D++DDDIEWEEAPIGG
Sbjct: 423 GMKAKREEDDDDDDIEWEEAPIGG 444

[Close]

BLAST of MS002099 vs. ExPASy TrEMBL
Match: A0A5A7UX12 (Transcription initiation factor IIE subunit alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003550 PE=3 SV=1)

HSP 1 Score: 788.5 bits (2035), Expect = 1.5e-224
Identity = 410/444 (92.34%), Postives = 424/444 (95.50%), Query Frame = 0


Query: 3   LDQLGRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62
           ++   RLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL
Sbjct: 3   IEPFNRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62

Query: 63  KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQQPGKEGDEKIKMHT 122
           KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQ   KEGDEKIKMHT
Sbjct: 63  KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQHQTKEGDEKIKMHT 122

Query: 123 HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE 182
           HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLIS E
Sbjct: 123 HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISFE 182

Query: 183 DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR 242
           DEYFHCENCNGELVAESDKLAA QGGDGDDNARKRRHEKLKDMLQKME QLKPL+EQL+R
Sbjct: 183 DEYFHCENCNGELVAESDKLAAVQGGDGDDNARKRRHEKLKDMLQKMEAQLKPLVEQLTR 242

Query: 243 VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA 302
           VKDLPVPEFGTLLAWEARASAAARG NGDLNGNDPSKSSQ  GYGGTPMPFVGETKVEVA
Sbjct: 243 VKDLPVPEFGTLLAWEARASAAARGVNGDLNGNDPSKSSQ--GYGGTPMPFVGETKVEVA 302

Query: 303 FSGAEGKGVDVKSETENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSA-AQFSDD 362
           FSGAEGKGVDVKSE++NTGLKVLPPWMIK+GMNLTKEQRGEVK+ESK DAGSA AQFSDD
Sbjct: 303 FSGAEGKGVDVKSESKNTGLKVLPPWMIKQGMNLTKEQRGEVKDESKTDAGSASAQFSDD 362

Query: 363 KKSSANDDDKTNFQDEYVKAYYAAILKKQQELEESAKRPEELSSTEVTESVSNANSSRQV 422
           KKS ANDDDKTN QDEYVKAYYAAILKKQQELEE+AK  +ELSSTEVTESV+N NSSR+V
Sbjct: 363 KKSLANDDDKTNIQDEYVKAYYAAILKKQQELEEAAKGQQELSSTEVTESVANTNSSRRV 422

Query: 423 GMKAKREGDEEDDDIEWEEAPIGG 446
           GMKAKRE D++DDDIEWEEAPIGG
Sbjct: 423 GMKAKREEDDDDDDIEWEEAPIGG 444

[Close]

BLAST of MS002099 vs. TAIR 10
Match: AT1G03280.1 (Transcription factor TFIIE, alpha subunit )

HSP 1 Score: 535.4 bits (1378), Expect = 4.3e-152
Identity = 289/443 (65.24%), Postives = 358/443 (80.81%), Query Frame = 0


Query: 8   RLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNLKLHSK 67
           +LV+L ARAFYDD TTK DNQ K+ RSDNRGIA VVLDAL RRQWVREEDLAK+L+LH+K
Sbjct: 18  KLVRLVARAFYDDYTTKSDNQQKSARSDNRGIAAVVLDALARRQWVREEDLAKDLQLHAK 77

Query: 68  QLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQQPGKEGDEKIKMHTHSYCS 127
           QLR+ +R  EEEKLI RDHR+ETAKGAK++SAAVAAT DG+      ++K+K+HTHSYC 
Sbjct: 78  QLRKIIRLFEEEKLIMRDHRKETAKGAKMYSAAVAATTDGR-----AEDKVKLHTHSYCC 137

Query: 128 LDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLEDEYFH 187
           LDYAQI DVVR+R+HRMKK+LKDELEDKNTVQEY CPNC R+Y ALDALRLIS+ D+ FH
Sbjct: 138 LDYAQICDVVRFRLHRMKKRLKDELEDKNTVQEYGCPNCQRKYNALDALRLISMVDDSFH 197

Query: 188 CENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSRVKDLP 247
           CENCNGELV E +KL + +  DGDDNAR+RR E LK+MLQK+EVQ+KPLM+QL+RVKDLP
Sbjct: 198 CENCNGELVVECNKLTSEEVVDGDDNARRRRRENLKNMLQKLEVQMKPLMDQLNRVKDLP 257

Query: 248 VPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVAFSGAE 307
           +PEFG+ LAWEARA+ AAR  NGDLN NDP + SQG GYG TPMPF+GETKVEV      
Sbjct: 258 IPEFGSFLAWEARAAMAAR-ENGDLNPNDPLR-SQG-GYGSTPMPFLGETKVEVNLGDG- 317

Query: 308 GKGVDVKSETENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMD--AGSAAQFSDDKKSS 367
               DVKS+  ++ LKVLPPWMIK+GMNLT+EQRGE+++E+K+D  AG+AA+ SDDKKS+
Sbjct: 318 --NEDVKSKGGDSSLKVLPPWMIKEGMNLTEEQRGEMRQEAKVDGGAGAAAKLSDDKKSA 377

Query: 368 ANDDDKTNFQDEYVKAYYAAILKKQQELEESAKRPEELSSTEVTESVSNAN--SSRQVGM 427
             + D+ + +DEY+KAYYA ++K+Q   E +A+R ++ S+ E T  + +    S RQV M
Sbjct: 378 IGNGDEKDLKDEYLKAYYAELMKQQ---ELAARRNQQESAGEPTSGIQSGTVYSGRQVSM 437

Query: 428 KAKREGDEEDD--DIEWEE-API 444
           KAKRE DE++D  ++EWEE AP+
Sbjct: 438 KAKREEDEDEDEEEVEWEEKAPV 446

[Close]

BLAST of MS002099 vs. TAIR 10
Match: AT4G20340.1 (Transcription factor TFIIE, alpha subunit )

HSP 1 Score: 444.9 bits (1143), Expect = 7.6e-125
Identity = 248/439 (56.49%), Postives = 321/439 (73.12%), Query Frame = 0


Query: 3   LDQLGRLVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNL 62
           L+   +LV+L  R FYD+ T + DNQ K+ + + +G AV+VLDALTRRQWVREEDLAK +
Sbjct: 14  LEPFVKLVRLLVRIFYDNYTPESDNQQKSVK-NVKGSAVIVLDALTRRQWVREEDLAKEV 73

Query: 63  KLHSKQLRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQQPGKEGDEKIKMHT 122
           K ++K+LR+ +R  EE+K + R HR+ETAK AK++S AV  T DG+      ++ +K HT
Sbjct: 74  KRNAKELRKLIRHFEEQKFVMRYHRKETAKRAKMYSYAVGGTTDGR-----AEDNVKFHT 133

Query: 123 HSYCSLDYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLE 182
           HSYC LDYAQIYD+VRY++HR+KKK KDELED+NTVQEY CPNC R+Y ALDALRLIS+E
Sbjct: 134 HSYCCLDYAQIYDIVRYKLHRLKKKFKDELEDRNTVQEYGCPNCKRKYNALDALRLISME 193

Query: 183 DEYFHCENCNGELVAESDKLAAAQGGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSR 242
           D+ FHCENCNGELV E +KL + +  D  DNAR+R+ EK+K  LQ +E +LKPLME ++R
Sbjct: 194 DDSFHCENCNGELVMECNKLISEEVVDRGDNARRRQREKVKVWLQDLEGELKPLMELINR 253

Query: 243 VKDLPVPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVA 302
           VKDLP P F    AWEARA+ AAR  NGD N +DPS+S    GYG TPMPF+GETKVEV 
Sbjct: 254 VKDLPFPAFEPFPAWEARAAKAAR-ENGDFNPDDPSRSLG--GYGSTPMPFLGETKVEVN 313

Query: 303 FSGAEGKGVDVKSETENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSAAQFSDDK 362
               EG   DV S   ++ LK+LPPWMIK+GM LT+EQRGE+++E+ +D G AA+ SDDK
Sbjct: 314 L--GEG-NEDVTSTGGDSSLKMLPPWMIKQGMKLTEEQRGEMRQEANVD-GEAAKLSDDK 373

Query: 363 KS-SANDDDKTNFQDEYVKAYYAAILKKQQELEESAKRPEELSSTEVTESVSNANSSRQV 422
           KS   N DD  + +DEY+KAYYAAI+++Q+   +  ++     ST      +   S RQV
Sbjct: 374 KSVMENGDDNKDLKDEYLKAYYAAIMEQQKLAAKLNEQESAGESTTTDIESATTYSDRQV 433

Query: 423 GMKAKREGDEEDDDIEWEE 441
           GMK+KRE  EE++D+EWEE
Sbjct: 434 GMKSKRE--EEEEDVEWEE 437

[Close]

BLAST of MS002099 vs. TAIR 10
Match: AT4G20810.1 (transcription initiation factor IIE (TFIIE) alpha subunit family protein / general transcription factor TFIIE family protein )

HSP 1 Score: 371.7 bits (953), Expect = 8.2e-103
Identity = 215/427 (50.35%), Postives = 288/427 (67.45%), Query Frame = 0


Query: 9   LVKLAARAFYDDITTKGDNQPKTGRSDNRGIAVVVLDALTRRQWVREEDLAKNLKLHSKQ 68
           +VKL A+ FYD+ T K +NQ K+ ++ + GIAV+VLDALTRRQWVREEDLAK LKL++KQ
Sbjct: 1   MVKLVAKTFYDNYTPKNNNQKKSAKNGSGGIAVLVLDALTRRQWVREEDLAKELKLNTKQ 60

Query: 69  LRRTLRFLEEEKLITRDHRRETAKGAKIFSAAVAATADGQQPGKEGDEKIKMHTHSYCSL 128
           LR  LR+ EE++ I R HR+E +          +AT +G+     G++K+K+H +SYC L
Sbjct: 61  LRTILRYFEEQQFIMRVHRKEKS----------SATTNGR-----GEDKVKVHMYSYCCL 120

Query: 129 DYAQIYDVVRYRMHRMKKKLKDELEDKNTVQEYICPNCSRRYTALDALRLISLEDEYFHC 188
           DY+QIYDV+RY++HRMKK+ KD LEDK+ VQEY CPNC R+               +FHC
Sbjct: 121 DYSQIYDVIRYKLHRMKKEFKDVLEDKDNVQEYGCPNCKRKI--------------FFHC 180

Query: 189 ENCNGELVAESDKLAAAQ-GGDGDDNARKRRHEKLKDMLQKMEVQLKPLMEQLSRVKDLP 248
           ENCNGELV E +KL + +   DG DN R RR + LKD+LQ MEV+LKPLM+ ++R+KDLP
Sbjct: 181 ENCNGELVMECNKLTSEEVVVDGSDNPRSRR-DHLKDLLQNMEVRLKPLMDHINRIKDLP 240

Query: 249 VPEFGTLLAWEARASAAARGANGDLNGNDPSKSSQGLGYGGTPMPFVGETKVEVAFSGAE 308
           VP F +  AWE R + AAR  NGDLN +D  +  QG GYG TPMPF+GET++EV   G E
Sbjct: 241 VPSFESFPAWETRVAKAAR-ENGDLNPDDTLR-PQG-GYGSTPMPFLGETEIEVNL-GEE 300

Query: 309 GKGVDVKSETENTGLKVLPPWMIKKGMNLTKEQRGEVKEESKMDAGSAAQFSDDKKSSAN 368
            + V      +++  K+ P W+IKKGMNL+ EQRGE++ E+K D G +        S  N
Sbjct: 301 NEDVKSDEVGDSSRRKLTPSWLIKKGMNLSDEQRGEIRHEAKADDGGS--------SMEN 360

Query: 369 DDDKTNFQDEYVKAYYAAILKKQQELEESAKRPEELSSTEVTESVSNANSSRQVGMKAKR 428
            DD  N +DEY+KAYYAAIL ++QEL E   + E          ++ ++S RQVGMK+KR
Sbjct: 361 GDDDRNLKDEYLKAYYAAIL-EEQELAEKLNQQESAGKVTTDIELATSSSDRQVGMKSKR 384

Query: 429 EGDEEDD 435
           E +EE++
Sbjct: 421 EEEEEEE 384

The following BLAST results are available for this feature:

BLAST of MS002099 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134956.1	1.4e-242	98.87	general transcription factor IIE subunit 1 [Momordica charantia]	[more]
XP_038880289.1	2.2e-230	95.05	general transcription factor IIE subunit 1 isoform X1 [Benincasa hispida]	[more]
XP_038880290.1	8.2e-230	95.03	general transcription factor IIE subunit 1 isoform X2 [Benincasa hispida]	[more]
XP_022922068.1	5.3e-229	93.47	general transcription factor IIE subunit 1-like [Cucurbita moschata] >KAG6589563...	[more]
XP_023515738.1	4.5e-228	93.02	general transcription factor IIE subunit 1-like [Cucurbita pepo subsp. pepo] >XP...	[more]

BLAST of MS002099 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q557M8	1.6e-18	25.45	General transcription factor IIE subunit 1 OS=Dictyostelium discoideum OX=44689 ...	[more]
P36100	2.3e-09	20.82	Transcription initiation factor IIE subunit alpha OS=Saccharomyces cerevisiae (s...	[more]
P29083	3.0e-09	25.85	General transcription factor IIE subunit 1 OS=Homo sapiens OX=9606 GN=GTF2E1 PE=...	[more]
Q5R8H5	4.0e-09	25.85	General transcription factor IIE subunit 1 OS=Pongo abelii OX=9601 GN=GTF2E1 PE=...	[more]
Q9P3W1	1.2e-08	23.48	Transcription initiation factor IIE subunit alpha OS=Schizosaccharomyces pombe (...	[more]

BLAST of MS002099 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BZ86	7.0e-243	98.87	Transcription initiation factor IIE subunit alpha OS=Momordica charantia OX=3673...	[more]
A0A6J1E5J2	2.6e-229	93.47	Transcription initiation factor IIE subunit alpha OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1JA07	1.4e-227	92.79	Transcription initiation factor IIE subunit alpha OS=Cucurbita maxima OX=3661 GN...	[more]
A0A5D3DYI4	1.1e-224	92.34	Transcription initiation factor IIE subunit alpha OS=Cucumis melo var. makuwa OX...	[more]
A0A5A7UX12	1.5e-224	92.34	Transcription initiation factor IIE subunit alpha OS=Cucumis melo var. makuwa OX...	[more]

BLAST of MS002099 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G03280.1	4.3e-152	65.24	Transcription factor TFIIE, alpha subunit	[more]
AT4G20340.1	7.6e-125	56.49	Transcription factor TFIIE, alpha subunit	[more]
AT4G20810.1	8.2e-103	50.35	transcription initiation factor IIE (TFIIE) alpha subunit family protein / gener...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 59..79
None	No IPR available	COILS	Coil	Coil	coord: 219..239
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 406..421
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 267..288
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 336..350
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 392..445
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 333..374
None	No IPR available	SUPERFAMILY	57783	Zinc beta-ribbon	coord: 147..200
IPR002853	Transcription initiation factor IIE subunit alpha, N-terminal	SMART	SM00531	tfiie3	coord: 38..209 e-value: 1.7E-62 score: 223.6
IPR024550	TFIIEalpha/SarR/Rpc3 HTH domain	PFAM	PF02002	TFIIE_alpha	coord: 40..93 e-value: 3.1E-7 score: 30.2
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 147..206 e-value: 1.3E-8 score: 36.1
IPR039997	Transcription factor E	PANTHER	PTHR13097	TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNIT	coord: 9..440
IPR017919	Transcription factor TFE/TFIIEalpha HTH domain	PROSITE	PS51344	HTH_TFE_IIE	coord: 6..138 score: 31.922421
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 42..126

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS002099.1	MS002099.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006367	transcription initiation from RNA polymerase II promoter