MS002079 (gene) Bitter gourd (TR) v1

Overview


Name	MS002079
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Phosphopyruvate hydratase
Location	scaffold30: 2237024 .. 2240049 (-)
RNA-Seq Expression	MS002079
Synteny	MS002079

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCTTGTCTTCCCCACCCGCCATGAATCTCCTCCACCACACTCTCCTTCCTTCCAAATCCCATTCTTTGATTGCCTCCATACCCTCTTCTTCTTCTTCTTCTTCTTCCTCCTCATCCTCCAACCATCTCCGCTCCCGCGCCGTCCGCTGCTCCGTTGCTGTGGCGCCTTCCGCCGCCACCAAGGCGTCCAAGGAATACAAGTTGAAGTCGGTCAAGGCCCGGCAGATCATCGACAGCAGAGGCAATCCCACGGTCGAGGTTGACCTCGTTACTGATGATCTCTACCGATCCGCTGTCCCCAGTGGGGCCTCCACTGGGATCTACGAGGCTTTGGAGCTCAGAGATGGGGATAAAAGCGTTTATGGAGGTAAAGGCGTGCTTACTGCTGTCAAGAACATCAATGACATCTTGGCTCCAAAGCTCGTCGGCGTCGATGTCAGGTGCAATTTGCAATTACGTCTATCTAATCAAATTATTTCTTCCACTTTCAATGAATATGAACATAATTCTGCTCTCTTAGTATATACCTCGTTTGGAAATTGACTGCAGATCGGATTTTATATTTAAGTTGACCATCTATACCATGTCTTATGAATCCACTTTCTAGGACTAAAATTGCGCAAAGAGATTTATCTCTGAAACTTCAAATACTTACGCTGCCTTTTCATCCTGGTTGGGATTTGTGTAGAAGGTGTGAGTTCTCGGGTGTGTTTGGGTGTGTTTGGAAGTGAGAGTAGAATGAGTGATTTTGAAATAAGAGATTTAAGGATAATTGATTTTCTAAAATCACATCAAATACCTTAAAAATCAATTTAAAGTGATTTTAAACTTTTTAAAATCAATTTTTAATTTGCCAAAACATGATCGGAGAGCGAAAATGATTTTGACAATGGCAATCAAAATCACTCCCAAACGGATGATTGTTGCCAACAAGGACATGAAGGGAAACAAAAGACTGGAAAAATTTGTAGCTCAAACTATGTGGTTTTTCGTTGAACTTACTAATATGTACTCTGACTGTGGAAATGTACCTCTTCTTACTTATGTCGTCAGTTGTTAATTGGTAGTGTTTTTATTCAATTTGTTCCTGAAGATGTAAAATTCCAAGCCTTTTTGTTGTTCCTTCTATGTGAAAGTTGTTTCGCCTTCCACTTCTGCTTCCTACCTAAGCTTGTCAACTTTCTCTAGAAATCAAGAAGATGTTGATGCAATTATGCTGGAAATTGATGGAACCACGAACAAGTCAAAACTTGGGGCAAATGCTATTCTAGGAGTTTCTTTGAGCGTATGCAGAGCTGGTGCTGGCGCCAAGGGACTACCGTTATATAGGCACATCCAAGAATTATCAGGCACAAAGGAGCTTGTTATGCCAGTTCCTGCTTTTAATGTTATAAATGGGGGTAGCCATGCTGGTAATAATTTAGCCATGCAAGAATTTATGATCTTACCAGTAGGTGCTACATCTTTTGCTGAGGCACTTCGCATGGGAAGTGAAGGTAAGAAGCTGCTTCTTTAAGTACTGCAATTTTCTTTTCCGTTGTTACTGTCATAGTTTATGATAGTCTCATATATAGTCTTGTTTCTGACTTATAAGCATGATTTAGGGACCATTTCTTCGACTTCTGTCGCTTTCTTACTTTCCTGTCATTAGCGAGTAAGAAATAGCCTGACTTTTGTGTTTTCGTATGAACAGTTTATCACATACTGAAGGGAATTATTAAAGCTAAATATGGCCAAGATGCTTGTAATGTTGGAGATGAGGGTGGGTTTGCTCCAAATGTTCAAGATAATAGAGAGGGACTGGTATTGCTAATTGATGCCATTGAAAAGGCTGGTTATACAGGAAAGGTACAGGATACTATATTTTCCTTTTTGTTTGGTATGGTGTAATTGTTTTCTTATGAGTCCTGCTAAGATAAAAAGGTTTCATTTTGATGCAGATCAAAATAGGAATGGATGTTGCAGCTTCAGAGTTTTTCACTAAAGATGGAAAATATGATCTGAACTTCAAGAAACAGCCAAATGATGGAGCTCATGTTCACTCAGCTCATGGCCTCGGCGAACTCTATAAACAGTTCATCAAAGATTTTCCTATTGTTTCCATCGAGGACCCATTTGACCAAGATGATTGGAGTTCCTGGTCTTCCCTACAATCTTCTGTTGATATCCAACTTGTAGGAGATGACTTGTTGGTTACAAATCCAAAGAGAATAGCGGAAGCCATTCATAAAAAGGCTTGTAATGCTTTACTTCTGAAGGTAAGAAGCCTCTTATTTGATTCTTCCGTTTCTCTCTGTTTCAAAATAATTCTACGATTCATCCTTAAAATTTCATTTGTTCTTTATCCCCTGAATCTTCAGGTTCTCCATTGACTTAGGCTGACCAATCTCTATATGCAGTTAGAAACATGTCATTTTGATACTGCAATTAGCTCATATGTTTGGAATTTATTTTGCAGGTGAACCAGATTGGATCAGTAACAGAATCAATTCAAGCTGCTCTTGATTCAAAGGCTGCTGGTTGGGGTGTGATGGTCAGCCACCGAAGTGGTGAAACTGAAGATAATTTTATTGCTGATCTTTCTGTTGGCTTGGCTAGTGGACAGGTTTCATTTATCTTCAGCTCTGCCATCTATAACTATAAAGCATCCATGTTGCTGACATAACATTCATAACTGAATATTGGGTTACTCTTTTACAGATCAAGACTGGAGCTCCATGCCGGAGTGAACGGTTAGCCAAATATAATCAGGTACAGTTCAAACTGTTTATCAAATTTGCAGACCTCTTCTGTAGGTTAACATTAACCAACTTGATCAGATTAACTTCCAAAATTAGTATAATGTTAAATGTTCTCTGTTTCCTGATGATGGTACTATTTCTTGAAATGTTTGCAAAGAGTTTCACCTATTGTACTCTAATTCAACACTAGTTCTAATTTTAAAAATGTGAATGCAGCTTCTTCGCATTGAAGAAGAACTTGGCAGCGTTAGCTATGCTGGCGAGTCTTTCAGATCGCCC

mRNA sequence

ATGGCTTCCTTGTCTTCCCCACCCGCCATGAATCTCCTCCACCACACTCTCCTTCCTTCCAAATCCCATTCTTTGATTGCCTCCATACCCTCTTCTTCTTCTTCTTCTTCTTCCTCCTCATCCTCCAACCATCTCCGCTCCCGCGCCGTCCGCTGCTCCGTTGCTGTGGCGCCTTCCGCCGCCACCAAGGCGTCCAAGGAATACAAGTTGAAGTCGGTCAAGGCCCGGCAGATCATCGACAGCAGAGGCAATCCCACGGTCGAGGTTGACCTCGTTACTGATGATCTCTACCGATCCGCTGTCCCCAGTGGGGCCTCCACTGGGATCTACGAGGCTTTGGAGCTCAGAGATGGGGATAAAAGCGTTTATGGAGGTAAAGGCGTGCTTACTGCTGTCAAGAACATCAATGACATCTTGGCTCCAAAGCTCGTCGGCGTCGATGTCAGAAATCAAGAAGATGTTGATGCAATTATGCTGGAAATTGATGGAACCACGAACAAGTCAAAACTTGGGGCAAATGCTATTCTAGGAGTTTCTTTGAGCGTATGCAGAGCTGGTGCTGGCGCCAAGGGACTACCGTTATATAGGCACATCCAAGAATTATCAGGCACAAAGGAGCTTGTTATGCCAGTTCCTGCTTTTAATGTTATAAATGGGGGTAGCCATGCTGGTAATAATTTAGCCATGCAAGAATTTATGATCTTACCAGTAGGTGCTACATCTTTTGCTGAGGCACTTCGCATGGGAAGTGAAGTTTATCACATACTGAAGGGAATTATTAAAGCTAAATATGGCCAAGATGCTTGTAATGTTGGAGATGAGGGTGGGTTTGCTCCAAATGTTCAAGATAATAGAGAGGGACTGGTATTGCTAATTGATGCCATTGAAAAGGCTGGTTATACAGGAAAGATCAAAATAGGAATGGATGTTGCAGCTTCAGAGTTTTTCACTAAAGATGGAAAATATGATCTGAACTTCAAGAAACAGCCAAATGATGGAGCTCATGTTCACTCAGCTCATGGCCTCGGCGAACTCTATAAACAGTTCATCAAAGATTTTCCTATTGTTTCCATCGAGGACCCATTTGACCAAGATGATTGGAGTTCCTGGTCTTCCCTACAATCTTCTGTTGATATCCAACTTGTAGGAGATGACTTGTTGGTTACAAATCCAAAGAGAATAGCGGAAGCCATTCATAAAAAGGCTTGTAATGCTTTACTTCTGAAGGTGAACCAGATTGGATCAGTAACAGAATCAATTCAAGCTGCTCTTGATTCAAAGGCTGCTGGTTGGGGTGTGATGGTCAGCCACCGAAGTGGTGAAACTGAAGATAATTTTATTGCTGATCTTTCTGTTGGCTTGGCTAGTGGACAGATCAAGACTGGAGCTCCATGCCGGAGTGAACGGTTAGCCAAATATAATCAGCTTCTTCGCATTGAAGAAGAACTTGGCAGCGTTAGCTATGCTGGCGAGTCTTTCAGATCGCCC

Coding sequence (CDS)

ATGGCTTCCTTGTCTTCCCCACCCGCCATGAATCTCCTCCACCACACTCTCCTTCCTTCCAAATCCCATTCTTTGATTGCCTCCATACCCTCTTCTTCTTCTTCTTCTTCTTCCTCCTCATCCTCCAACCATCTCCGCTCCCGCGCCGTCCGCTGCTCCGTTGCTGTGGCGCCTTCCGCCGCCACCAAGGCGTCCAAGGAATACAAGTTGAAGTCGGTCAAGGCCCGGCAGATCATCGACAGCAGAGGCAATCCCACGGTCGAGGTTGACCTCGTTACTGATGATCTCTACCGATCCGCTGTCCCCAGTGGGGCCTCCACTGGGATCTACGAGGCTTTGGAGCTCAGAGATGGGGATAAAAGCGTTTATGGAGGTAAAGGCGTGCTTACTGCTGTCAAGAACATCAATGACATCTTGGCTCCAAAGCTCGTCGGCGTCGATGTCAGAAATCAAGAAGATGTTGATGCAATTATGCTGGAAATTGATGGAACCACGAACAAGTCAAAACTTGGGGCAAATGCTATTCTAGGAGTTTCTTTGAGCGTATGCAGAGCTGGTGCTGGCGCCAAGGGACTACCGTTATATAGGCACATCCAAGAATTATCAGGCACAAAGGAGCTTGTTATGCCAGTTCCTGCTTTTAATGTTATAAATGGGGGTAGCCATGCTGGTAATAATTTAGCCATGCAAGAATTTATGATCTTACCAGTAGGTGCTACATCTTTTGCTGAGGCACTTCGCATGGGAAGTGAAGTTTATCACATACTGAAGGGAATTATTAAAGCTAAATATGGCCAAGATGCTTGTAATGTTGGAGATGAGGGTGGGTTTGCTCCAAATGTTCAAGATAATAGAGAGGGACTGGTATTGCTAATTGATGCCATTGAAAAGGCTGGTTATACAGGAAAGATCAAAATAGGAATGGATGTTGCAGCTTCAGAGTTTTTCACTAAAGATGGAAAATATGATCTGAACTTCAAGAAACAGCCAAATGATGGAGCTCATGTTCACTCAGCTCATGGCCTCGGCGAACTCTATAAACAGTTCATCAAAGATTTTCCTATTGTTTCCATCGAGGACCCATTTGACCAAGATGATTGGAGTTCCTGGTCTTCCCTACAATCTTCTGTTGATATCCAACTTGTAGGAGATGACTTGTTGGTTACAAATCCAAAGAGAATAGCGGAAGCCATTCATAAAAAGGCTTGTAATGCTTTACTTCTGAAGGTGAACCAGATTGGATCAGTAACAGAATCAATTCAAGCTGCTCTTGATTCAAAGGCTGCTGGTTGGGGTGTGATGGTCAGCCACCGAAGTGGTGAAACTGAAGATAATTTTATTGCTGATCTTTCTGTTGGCTTGGCTAGTGGACAGATCAAGACTGGAGCTCCATGCCGGAGTGAACGGTTAGCCAAATATAATCAGCTTCTTCGCATTGAAGAAGAACTTGGCAGCGTTAGCTATGCTGGCGAGTCTTTCAGATCGCCC

Protein sequence

MASLSSPPAMNLLHHTLLPSKSHSLIASIPSSSSSSSSSSSSNHLRSRAVRCSVAVAPSAATKASKEYKLKSVKARQIIDSRGNPTVEVDLVTDDLYRSAVPSGASTGIYEALELRDGDKSVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEALRMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIEDPFDQDDWSSWSSLQSSVDIQLVGDDLLVTNPKRIAEAIHKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGSVSYAGESFRSP

Homology

[Close]

BLAST of MS002079 vs. NCBI nr
Match: XP_022134829.1 (enolase 1, chloroplastic [Momordica charantia])

HSP 1 Score: 952.6 bits (2461), Expect = 1.4e-273
Identity = 494/496 (99.60%), Postives = 494/496 (99.60%), Query Frame = 0


Query: 1   MASLSSPPAMNLLHHTLLPSKSHSLIASIPSSSSSSSSSSSSNHLRSRAVRCSVAVAPSA 60
           MASLSSPPAMNLLHHTLLPSKSHSLIASIPSSSSSSSSSSSSNHLRSRAVRCSVAVAPSA
Sbjct: 1   MASLSSPPAMNLLHHTLLPSKSHSLIASIPSSSSSSSSSSSSNHLRSRAVRCSVAVAPSA 60

Query: 61  ATKASKEYKLKSVKARQIIDSRGNPTVEVDLVTDDLYRSAVPSGASTGIYEALELRDGDK 120
           ATKASKEYKLKSVKARQIIDSRGNPTVEVDLVTDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  ATKASKEYKLKSVKARQIIDSRGNPTVEVDLVTDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 SVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSL 180
           SVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSL
Sbjct: 121 SVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT 240

Query: 241 SFAEALRMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIED 360
           TGKIKIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIED 360

Query: 361 PFDQDDWSSWSSLQSSVDIQLVGDDLLVTNPKRIAEAIHKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSWSSLQSSVDIQLVGDDLLVTNPKRIAEAIHKKAC ALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWSSLQSSVDIQLVGDDLLVTNPKRIAEAIHKKACTALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVSYAGESFRSP 497
           EELGSV YAGESFRSP
Sbjct: 481 EELGSVRYAGESFRSP 496

[Close]

BLAST of MS002079 vs. NCBI nr
Match: XP_038879680.1 (enolase 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 884.8 bits (2285), Expect = 3.5e-253
Identity = 456/496 (91.94%), Postives = 471/496 (94.96%), Query Frame = 0


Query: 1   MASLSSPPAMNLLHHTLLPSKSHSLIASIPSSSSSSSSSSSSNHLRSRAVRCSVAVAPSA 60
           MASL SPP MNLL  +LLPS   S I+SIPSSSSSS        LRS A+RCSVAVAPS 
Sbjct: 1   MASLPSPPTMNLLEKSLLPSSPQSFISSIPSSSSSSKP------LRSHAIRCSVAVAPSV 60

Query: 61  ATKASKEYKLKSVKARQIIDSRGNPTVEVDLVTDDLYRSAVPSGASTGIYEALELRDGDK 120
           +TKASKE+KLKSVKARQIIDSRGNPTVEVDL+TDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  STKASKEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 SVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSL 180
           +VYGGKGVLTAVKNINDILAPKLVGVDVRNQE+VDAIMLEIDGT NKSKLGANAILGVSL
Sbjct: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA 
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAI 240

Query: 241 SFAEALRMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYH LKGIIKAKYGQDACNVGDEGGFAPNVQDN+EGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNKEGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIED 360
           TGKIKIGMDVAASEFFTK+GKYDLNFKKQPNDGAHVHSAHGLGELYKQF+KDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHGLGELYKQFVKDFPIVSIED 360

Query: 361 PFDQDDWSSWSSLQSSVDIQLVGDDLLVTNPKRIAEAIHKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSW+SLQSSVDIQLVGDDLLVTNPKRIA+ I KKACNALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAKGIQKKACNALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVSYAGESFRSP 497
           EE+GS+ YAG SFRSP
Sbjct: 481 EEIGSIRYAGASFRSP 490

[Close]

BLAST of MS002079 vs. NCBI nr
Match: TYK28380.1 (enolase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 884.4 bits (2284), Expect = 4.5e-253
Identity = 459/496 (92.54%), Postives = 470/496 (94.76%), Query Frame = 0


Query: 1   MASLSSPPAMNLLHHTLLPSKSHSLIASIPSSSSSSSSSSSSNHLRSRAVRCSVAVAPSA 60
           MASL SPP MNLL+  L  S   S I SIPSSSSSSSSSSSS  LRS  +RCSVAVAPS 
Sbjct: 1   MASLPSPPTMNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKPLRSPTIRCSVAVAPSV 60

Query: 61  ATKASKEYKLKSVKARQIIDSRGNPTVEVDLVTDDLYRSAVPSGASTGIYEALELRDGDK 120
           +T  SKE+KLKS+KARQIIDSRGNPTVEVDL+TDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 SVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSL 180
           +VYGGKGVLTAVKNINDILAPKLVGVDVRNQE+VDAIMLEIDGT NKSKLGANAILGVSL
Sbjct: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA+
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240

Query: 241 SFAEALRMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYH LKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIED 360
           TGKIKIGMDVAASEFFTK GKYDLNFKKQPNDGAHVHSAH LGELYKQF+KDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSIED 360

Query: 361 PFDQDDWSSWSSLQSSVDIQLVGDDLLVTNPKRIAEAIHKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSW+SLQSSVDIQLVGDDLLVTNPKRIAE I KKACNALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVSYAGESFRSP 497
           EELGSV YAG SFRSP
Sbjct: 481 EELGSVRYAGASFRSP 496

[Close]

BLAST of MS002079 vs. NCBI nr
Match: XP_008453338.1 (PREDICTED: enolase 1, chloroplastic [Cucumis melo] >KAA0058034.1 enolase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 883.2 bits (2281), Expect = 1.0e-252
Identity = 458/496 (92.34%), Postives = 470/496 (94.76%), Query Frame = 0


Query: 1   MASLSSPPAMNLLHHTLLPSKSHSLIASIPSSSSSSSSSSSSNHLRSRAVRCSVAVAPSA 60
           MASL SPP MNLL+  L  S   S I SIPSSSSSSSSSSSS  LRS  +RCSVAVAPS 
Sbjct: 1   MASLPSPPTMNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKPLRSPTIRCSVAVAPSV 60

Query: 61  ATKASKEYKLKSVKARQIIDSRGNPTVEVDLVTDDLYRSAVPSGASTGIYEALELRDGDK 120
           +T  SKE+KLKS+KARQIIDSRGNPTVEVDL+TDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 SVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSL 180
           +VYGGKGVLTAVKNI DILAPKLVGVDVRNQE+VDAIMLEIDGT NKSKLGANAILGVSL
Sbjct: 121 TVYGGKGVLTAVKNIKDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA+
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240

Query: 241 SFAEALRMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYH LKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIED 360
           TGKIKIGMDVAASEFFTK+GKYDLNFKKQPNDGAHVHSAH LGELYKQF+KDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSIED 360

Query: 361 PFDQDDWSSWSSLQSSVDIQLVGDDLLVTNPKRIAEAIHKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSW+SLQSSVDIQLVGDDLLVTNPKRIAE I KKACNALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVSYAGESFRSP 497
           EELGSV YAG SFRSP
Sbjct: 481 EELGSVRYAGASFRSP 496

[Close]

BLAST of MS002079 vs. NCBI nr
Match: KAG6587623.1 (Enolase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 876.3 bits (2263), Expect = 1.2e-250
Identity = 456/498 (91.57%), Postives = 469/498 (94.18%), Query Frame = 0


Query: 1   MASLSSPP--AMNLLHHTLLPSKSHSLIASIPSSSSSSSSSSSSNHLRSRAVRCSVAVAP 60
           MASL SPP    NLL+ +LLPSK  S I SIPSSSSS         LRS  VRCSVAVAP
Sbjct: 1   MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKP-------LRSHGVRCSVAVAP 60

Query: 61  SAATKASKEYKLKSVKARQIIDSRGNPTVEVDLVTDDLYRSAVPSGASTGIYEALELRDG 120
           SA+TKAS+E+KLKSVKARQIIDSRGNPTVEVDL+TDDLYRSAVPSGASTGIYEALELRDG
Sbjct: 61  SASTKASREFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDG 120

Query: 121 DKSVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGV 180
           DK+VYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGV
Sbjct: 121 DKNVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGV 180

Query: 181 SLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVG 240
           SLSVCRAGAGAKGLPLYRHIQELS TKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVG
Sbjct: 181 SLSVCRAGAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVG 240

Query: 241 ATSFAEALRMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKA 300
           A SFAEALRMGSEVYH LKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLL DAIEKA
Sbjct: 241 AASFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKA 300

Query: 301 GYTGKIKIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSI 360
           GYTGKIKIGMDVAASEFFTKDG+YDLNFKKQPNDGAHVHSAHGLGELYKQF+ DFPIVSI
Sbjct: 301 GYTGKIKIGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSI 360

Query: 361 EDPFDQDDWSSWSSLQSSVDIQLVGDDLLVTNPKRIAEAIHKKACNALLLKVNQIGSVTE 420
           EDPFDQDDWSSW+SLQSSV +Q+VGDDLLVTNPKRIAEAI KKACNALLLKVNQIGSVTE
Sbjct: 361 EDPFDQDDWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTE 420

Query: 421 SIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLR 480
           SIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLR
Sbjct: 421 SIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLR 480

Query: 481 IEEELGSVSYAGESFRSP 497
           IEEELG+V YAGESFRSP
Sbjct: 481 IEEELGNVRYAGESFRSP 491

[Close]

BLAST of MS002079 vs. ExPASy Swiss-Prot
Match: Q9C9C4 (Enolase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ENO1 PE=1 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 7.1e-225
Identity = 404/484 (83.47%), Postives = 434/484 (89.67%), Query Frame = 0


Query: 18  LPSKSHSLIASIPSSSSSS-----SSSSSSNHLRSRAVRCSVAVAPSAATKASKEYKLKS 77
           L +K H L  S  S S  S      S+ S    R   V+CSV          +KE ++K 
Sbjct: 3   LTTKPHHLQRSFLSPSRVSGERYLESAPSCLRFRRSGVQCSV---------VAKECRVKG 62

Query: 78  VKARQIIDSRGNPTVEVDLVTDDLYRSAVPSGASTGIYEALELRDGDKSVYGGKGVLTAV 137
           VKARQIIDSRGNPTVEVDL+TDDLYRSAVPSGASTGIYEALELRDGDKSVYGGKGVL A+
Sbjct: 63  VKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKSVYGGKGVLQAI 122

Query: 138 KNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRAGAGAKGL 197
           KNIN+++APKL+GVDVRNQ DVDA+MLE+DGT NKSKLGANAILGVSLSVCRAGAGAKG+
Sbjct: 123 KNINELVAPKLIGVDVRNQADVDALMLELDGTPNKSKLGANAILGVSLSVCRAGAGAKGV 182

Query: 198 PLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEALRMGSEV 257
           PLY+HIQE SGTKELVMPVPAFNVINGGSHAGN+LAMQEFMILPVGATSF+EA +MGSEV
Sbjct: 183 PLYKHIQETSGTKELVMPVPAFNVINGGSHAGNSLAMQEFMILPVGATSFSEAFQMGSEV 242

Query: 258 YHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAA 317
           YH LKGIIK KYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAA
Sbjct: 243 YHTLKGIIKTKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAA 302

Query: 318 SEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIEDPFDQDDWSSWSS 377
           SEFF KDG+YDLNFKKQPNDGAHV SA  L +LY++FIKDFPIVSIEDPFDQDDWSSW+S
Sbjct: 303 SEFFMKDGRYDLNFKKQPNDGAHVLSAESLADLYREFIKDFPIVSIEDPFDQDDWSSWAS 362

Query: 378 LQSSVDIQLVGDDLLVTNPKRIAEAIHKKACNALLLKVNQIGSVTESIQAALDSKAAGWG 437
           LQSSVDIQLVGDDLLVTNPKRIAEAI K++CNALLLKVNQIG+VTESIQAALDSKAAGWG
Sbjct: 363 LQSSVDIQLVGDDLLVTNPKRIAEAIKKQSCNALLLKVNQIGTVTESIQAALDSKAAGWG 422

Query: 438 VMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGSVSYAGES 497
           VMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERL+KYNQLLRIEEELG+V YAGE+
Sbjct: 423 VMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLSKYNQLLRIEEELGNVRYAGEA 477

[Close]

BLAST of MS002079 vs. ExPASy Swiss-Prot
Match: P42894 (Enolase OS=Neocallimastix frontalis OX=4757 PE=3 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 2.5e-169
Identity = 307/434 (70.74%), Postives = 360/434 (82.95%), Query Frame = 0


Query: 70  LKSVKARQIIDSRGNPTVEVDLVTD-DLYRSAVPSGASTGIYEALELRDGDKSVYGGKGV 129
           +  V ARQI DSRGNPTVEV++ TD  L+R+AVPSGASTG++EALELRDG K+ Y GKGV
Sbjct: 3   ITKVHARQIFDSRGNPTVEVEVTTDKGLFRAAVPSGASTGVHEALELRDGIKADYVGKGV 62

Query: 130 LTAVKNINDILAPKLV--GVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRAG 189
           L AV+N+N  +AP LV   +DV+NQ+ VD  +L++DGT NKSKLGANAILGVSL+V RAG
Sbjct: 63  LKAVENVNKTIAPALVAANLDVKNQKAVDDFLLKLDGTPNKSKLGANAILGVSLAVARAG 122

Query: 190 AGAKGLPLYRHIQELSGTK-ELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEA 249
           A  KG+PLY+H+ EL+G K   ++PVP+ NV+NGGSHAGN LAMQEFMILP GA SF EA
Sbjct: 123 AADKGVPLYQHLGELAGNKGPWILPVPSMNVLNGGSHAGNKLAMQEFMILPTGAKSFTEA 182

Query: 250 LRMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIK 309
           L+MGSEVYH LK +IKAKYGQDACNVGDEGGFAPN+QDN+EGL LL +AI KAGYTGK+K
Sbjct: 183 LKMGSEVYHALKSVIKAKYGQDACNVGDEGGFAPNIQDNKEGLELLNEAIAKAGYTGKVK 242

Query: 310 IGMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIEDPFDQD 369
           IGMDVA+SEF+ KDGKYDL+FK   +D +   S   LG+ YK+   ++PIVSIEDP+DQD
Sbjct: 243 IGMDVASSEFY-KDGKYDLDFKNPNSDPSKWISGEELGQFYKEITSEYPIVSIEDPYDQD 302

Query: 370 DWSSWSSLQSSVD--IQLVGDDLLVTNPKRIAEAIHKKACNALLLKVNQIGSVTESIQAA 429
           D+ SWS  ++ +   IQ+VGDDL VTNPKRIA AI KKACN LLLKVNQIG+V+ESIQAA
Sbjct: 303 DFESWSKFRADMQDKIQIVGDDLTVTNPKRIAMAIEKKACNGLLLKVNQIGTVSESIQAA 362

Query: 430 LDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEEL 489
           LD+   GWGVMVSHRSGETED FIADL VGL SGQIKTGAPCRSERLAKYNQLLRIEEEL
Sbjct: 363 LDAFNDGWGVMVSHRSGETEDTFIADLVVGLKSGQIKTGAPCRSERLAKYNQLLRIEEEL 422

Query: 490 G-SVSYAGESFRSP 497
           G + +YAGE+FR P
Sbjct: 423 GANATYAGENFRRP 435

[Close]

BLAST of MS002079 vs. ExPASy Swiss-Prot
Match: Q27527 (Enolase OS=Caenorhabditis elegans OX=6239 GN=enol-1 PE=1 SV=3)

HSP 1 Score: 595.9 bits (1535), Expect = 4.2e-169
Identity = 307/431 (71.23%), Postives = 355/431 (82.37%), Query Frame = 0


Query: 70  LKSVKARQIIDSRGNPTVEVDLVTD-DLYRSAVPSGASTGIYEALELRDGDKSVYGGKGV 129
           +  + ARQI DSRGNPTVEVDL T+  ++R+AVPSGASTG++EALELRDGDK+V+ GKGV
Sbjct: 3   ITKIHARQIYDSRGNPTVEVDLFTEKGVFRAAVPSGASTGVHEALELRDGDKAVHLGKGV 62

Query: 130 LTAVKNINDILAPKLV--GVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRAG 189
           L AV NIN+ +AP L+  G DV  Q+D+D  M+ +DG+ NK  LGANAILGVSL+V +AG
Sbjct: 63  LKAVSNINEKIAPALIAKGFDVTAQKDIDDFMMALDGSENKGNLGANAILGVSLAVAKAG 122

Query: 190 AGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEAL 249
           A  KGLPLY++I EL+GT ++V+PVPAFNVINGGSHAGN LAMQEFMILPVGA+SFAEA+
Sbjct: 123 AVHKGLPLYKYIAELAGTGKVVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFAEAM 182

Query: 250 RMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKI 309
           RMGSEVYH LK  IK +YG DA  VGDEGGFAPN+QDN+EGL LL  AI+KAGYTGKI I
Sbjct: 183 RMGSEVYHHLKAEIKKRYGLDATAVGDEGGFAPNIQDNKEGLDLLNTAIDKAGYTGKISI 242

Query: 310 GMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIEDPFDQDD 369
           GMDVAASEFF KDGKYDL+FK   +D +   S   L ELY+ FIK++P+VSIED FDQDD
Sbjct: 243 GMDVAASEFF-KDGKYDLDFKNPASDSSKWLSGEQLTELYQSFIKEYPVVSIEDAFDQDD 302

Query: 370 WSSWSSLQSSVDIQLVGDDLLVTNPKRIAEAIHKKACNALLLKVNQIGSVTESIQAALDS 429
           W +W     +  IQLVGDDL VTNPKRI  AI KK+CN LLLKVNQIGSVTESI+AA  S
Sbjct: 303 WDNWGKFHGATSIQLVGDDLTVTNPKRIQTAIDKKSCNCLLLKVNQIGSVTESIEAAKLS 362

Query: 430 KAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGS- 489
           +A GWGVMVSHRSGETED FIADL VGLA+GQIKTGAPCRSERLAKYNQLLRIEEELG+ 
Sbjct: 363 RANGWGVMVSHRSGETEDTFIADLVVGLATGQIKTGAPCRSERLAKYNQLLRIEEELGAD 422

Query: 490 VSYAGESFRSP 497
             YAG +FR+P
Sbjct: 423 AVYAGHNFRNP 432

[Close]

BLAST of MS002079 vs. ExPASy Swiss-Prot
Match: Q54RK5 (Enolase A OS=Dictyostelium discoideum OX=44689 GN=enoA PE=3 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 7.2e-169
Identity = 303/431 (70.30%), Postives = 359/431 (83.29%), Query Frame = 0


Query: 70  LKSVKARQIIDSRGNPTVEVDLVTD-----DLYRSAVPSGASTGIYEALELRDGDKSVYG 129
           +KS+KAR+I++SRGNPTVEVDL T+       +R+AVPSGASTGIYEA+ELRDGDKS Y 
Sbjct: 4   IKSIKAREILNSRGNPTVEVDLYTEKDGVVSSFRAAVPSGASTGIYEAVELRDGDKSRYL 63

Query: 130 GKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCR 189
           GKGVL A+KNI +++ P ++G  V +Q  +D +M+++DGT NK KLGANAIL VSL+VCR
Sbjct: 64  GKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTPNKGKLGANAILAVSLAVCR 123

Query: 190 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAE 249
           AGA  + LPLY++I E++GTK + +PVPAFNVINGGSHAGN LAMQEFMILPVGA  F E
Sbjct: 124 AGAADRNLPLYKYISEIAGTK-MRLPVPAFNVINGGSHAGNKLAMQEFMILPVGAKDFNE 183

Query: 250 ALRMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 309
           A RMGSEVYH LK +I  +YGQDA NVGDEGGFAP +Q N+EGL LL  AIEKAGYTG +
Sbjct: 184 AYRMGSEVYHNLKNVISGRYGQDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLV 243

Query: 310 KIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIEDPFDQ 369
           KIGMD AASEF  ++G YDL+FK + NDG+ V S   LG+LY++FIK++PI+SIEDPFDQ
Sbjct: 244 KIGMDCAASEFKVENG-YDLDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQ 303

Query: 370 DDWSSWSSLQSSVDIQLVGDDLLVTNPKRIAEAIHKKACNALLLKVNQIGSVTESIQAAL 429
           DDW S++ L +SVDIQ+VGDDLLVTNP+RI   I KKACNALLLKVNQIGSVTESI+AAL
Sbjct: 304 DDWESYTKLTASVDIQIVGDDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAAL 363

Query: 430 DSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELG 489
           DSK A WGVMVSHRSGETED FIADL VGL +GQIKTGAPCRSERLAKYNQL+RI EELG
Sbjct: 364 DSKNASWGVMVSHRSGETEDTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELG 423

Query: 490 -SVSYAGESFR 495
            + +YAG +FR
Sbjct: 424 ENHNYAGLTFR 432

[Close]

BLAST of MS002079 vs. ExPASy Swiss-Prot
Match: P15429 (Beta-enolase OS=Rattus norvegicus OX=10116 GN=Eno3 PE=1 SV=3)

HSP 1 Score: 594.3 bits (1531), Expect = 1.2e-168
Identity = 305/431 (70.77%), Postives = 358/431 (83.06%), Query Frame = 0


Query: 70  LKSVKARQIIDSRGNPTVEVDLVT-DDLYRSAVPSGASTGIYEALELRDGDKSVYGGKGV 129
           ++ + AR+I+DSRGNPTVEVDL T    +R+AVPSGASTGIYEALELRDGDKS Y GKGV
Sbjct: 3   MQKIFAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKSRYLGKGV 62

Query: 130 LTAVKNINDILAPKLV--GVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRAG 189
           L AV++IN  L P L+   + V +QE VD  M+E+DGT NKSK GANAILGVSL+VC+AG
Sbjct: 63  LKAVEHINKTLGPALLEKKLSVVDQEKVDKFMIELDGTENKSKFGANAILGVSLAVCKAG 122

Query: 190 AGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEAL 249
           A  KG+PLYRHI +L+G  +LV+PVPAFNVINGGSHAGN LAMQEFMILPVGA+SF EA+
Sbjct: 123 AAEKGVPLYRHIADLAGNPDLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAM 182

Query: 250 RMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKI 309
           R+G+EVYH LKG+IKAKYG+DA NVGDEGGFAPN+ +N E L LL  AI+ AGY  K+ I
Sbjct: 183 RIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVI 242

Query: 310 GMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIEDPFDQDD 369
           GMDVAASEF+ ++GKYDL+F K P+D A   S   LGELYK FIK++P+VSIEDPFDQDD
Sbjct: 243 GMDVAASEFY-RNGKYDLDF-KSPDDPARHISGEKLGELYKSFIKNYPVVSIEDPFDQDD 302

Query: 370 WSSWSSLQSSVDIQLVGDDLLVTNPKRIAEAIHKKACNALLLKVNQIGSVTESIQAALDS 429
           W++W+S  S VDIQ+VGDDL VTNPKRIA+A+ KKACN LLLKVNQIGSVTESIQA   +
Sbjct: 303 WATWTSFLSGVDIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSVTESIQACKLA 362

Query: 430 KAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGSV 489
           ++ GWGVMVSHRSGETED FIADL VGL +GQIKTGAPCRSERLAKYNQL+RIEE LG  
Sbjct: 363 QSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDK 422

Query: 490 S-YAGESFRSP 497
           + +AG  FR+P
Sbjct: 423 AVFAGRKFRNP 431

[Close]

BLAST of MS002079 vs. ExPASy TrEMBL
Match: A0A6J1BZW1 (Phosphopyruvate hydratase OS=Momordica charantia OX=3673 GN=LOC111007005 PE=3 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 6.5e-274
Identity = 494/496 (99.60%), Postives = 494/496 (99.60%), Query Frame = 0


Query: 1   MASLSSPPAMNLLHHTLLPSKSHSLIASIPSSSSSSSSSSSSNHLRSRAVRCSVAVAPSA 60
           MASLSSPPAMNLLHHTLLPSKSHSLIASIPSSSSSSSSSSSSNHLRSRAVRCSVAVAPSA
Sbjct: 1   MASLSSPPAMNLLHHTLLPSKSHSLIASIPSSSSSSSSSSSSNHLRSRAVRCSVAVAPSA 60

Query: 61  ATKASKEYKLKSVKARQIIDSRGNPTVEVDLVTDDLYRSAVPSGASTGIYEALELRDGDK 120
           ATKASKEYKLKSVKARQIIDSRGNPTVEVDLVTDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  ATKASKEYKLKSVKARQIIDSRGNPTVEVDLVTDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 SVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSL 180
           SVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSL
Sbjct: 121 SVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT 240

Query: 241 SFAEALRMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIED 360
           TGKIKIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIED 360

Query: 361 PFDQDDWSSWSSLQSSVDIQLVGDDLLVTNPKRIAEAIHKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSWSSLQSSVDIQLVGDDLLVTNPKRIAEAIHKKAC ALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWSSLQSSVDIQLVGDDLLVTNPKRIAEAIHKKACTALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVSYAGESFRSP 497
           EELGSV YAGESFRSP
Sbjct: 481 EELGSVRYAGESFRSP 496

[Close]

BLAST of MS002079 vs. ExPASy TrEMBL
Match: A0A5D3DYS6 (Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00060 PE=3 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 2.2e-253
Identity = 459/496 (92.54%), Postives = 470/496 (94.76%), Query Frame = 0


Query: 1   MASLSSPPAMNLLHHTLLPSKSHSLIASIPSSSSSSSSSSSSNHLRSRAVRCSVAVAPSA 60
           MASL SPP MNLL+  L  S   S I SIPSSSSSSSSSSSS  LRS  +RCSVAVAPS 
Sbjct: 1   MASLPSPPTMNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKPLRSPTIRCSVAVAPSV 60

Query: 61  ATKASKEYKLKSVKARQIIDSRGNPTVEVDLVTDDLYRSAVPSGASTGIYEALELRDGDK 120
           +T  SKE+KLKS+KARQIIDSRGNPTVEVDL+TDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 SVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSL 180
           +VYGGKGVLTAVKNINDILAPKLVGVDVRNQE+VDAIMLEIDGT NKSKLGANAILGVSL
Sbjct: 121 TVYGGKGVLTAVKNINDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA+
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240

Query: 241 SFAEALRMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYH LKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIED 360
           TGKIKIGMDVAASEFFTK GKYDLNFKKQPNDGAHVHSAH LGELYKQF+KDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSIED 360

Query: 361 PFDQDDWSSWSSLQSSVDIQLVGDDLLVTNPKRIAEAIHKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSW+SLQSSVDIQLVGDDLLVTNPKRIAE I KKACNALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVSYAGESFRSP 497
           EELGSV YAG SFRSP
Sbjct: 481 EELGSVRYAGASFRSP 496

[Close]

BLAST of MS002079 vs. ExPASy TrEMBL
Match: A0A5A7US84 (Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003390 PE=3 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 4.9e-253
Identity = 458/496 (92.34%), Postives = 470/496 (94.76%), Query Frame = 0


Query: 1   MASLSSPPAMNLLHHTLLPSKSHSLIASIPSSSSSSSSSSSSNHLRSRAVRCSVAVAPSA 60
           MASL SPP MNLL+  L  S   S I SIPSSSSSSSSSSSS  LRS  +RCSVAVAPS 
Sbjct: 1   MASLPSPPTMNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKPLRSPTIRCSVAVAPSV 60

Query: 61  ATKASKEYKLKSVKARQIIDSRGNPTVEVDLVTDDLYRSAVPSGASTGIYEALELRDGDK 120
           +T  SKE+KLKS+KARQIIDSRGNPTVEVDL+TDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 SVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSL 180
           +VYGGKGVLTAVKNI DILAPKLVGVDVRNQE+VDAIMLEIDGT NKSKLGANAILGVSL
Sbjct: 121 TVYGGKGVLTAVKNIKDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA+
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240

Query: 241 SFAEALRMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYH LKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIED 360
           TGKIKIGMDVAASEFFTK+GKYDLNFKKQPNDGAHVHSAH LGELYKQF+KDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSIED 360

Query: 361 PFDQDDWSSWSSLQSSVDIQLVGDDLLVTNPKRIAEAIHKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSW+SLQSSVDIQLVGDDLLVTNPKRIAE I KKACNALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVSYAGESFRSP 497
           EELGSV YAG SFRSP
Sbjct: 481 EELGSVRYAGASFRSP 496

[Close]

BLAST of MS002079 vs. ExPASy TrEMBL
Match: A0A1S3BX56 (Phosphopyruvate hydratase OS=Cucumis melo OX=3656 GN=LOC103494088 PE=3 SV=1)

HSP 1 Score: 883.2 bits (2281), Expect = 4.9e-253
Identity = 458/496 (92.34%), Postives = 470/496 (94.76%), Query Frame = 0


Query: 1   MASLSSPPAMNLLHHTLLPSKSHSLIASIPSSSSSSSSSSSSNHLRSRAVRCSVAVAPSA 60
           MASL SPP MNLL+  L  S   S I SIPSSSSSSSSSSSS  LRS  +RCSVAVAPS 
Sbjct: 1   MASLPSPPTMNLLNKPLSLSPPQSFIVSIPSSSSSSSSSSSSKPLRSPTIRCSVAVAPSV 60

Query: 61  ATKASKEYKLKSVKARQIIDSRGNPTVEVDLVTDDLYRSAVPSGASTGIYEALELRDGDK 120
           +T  SKE+KLKS+KARQIIDSRGNPTVEVDL+TDDLYRSAVPSGASTGIYEALELRDGDK
Sbjct: 61  STNVSKEFKLKSLKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDK 120

Query: 121 SVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSL 180
           +VYGGKGVLTAVKNI DILAPKLVGVDVRNQE+VDAIMLEIDGT NKSKLGANAILGVSL
Sbjct: 121 TVYGGKGVLTAVKNIKDILAPKLVGVDVRNQEEVDAIMLEIDGTPNKSKLGANAILGVSL 180

Query: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAT 240
           SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGA+
Sbjct: 181 SVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGAS 240

Query: 241 SFAEALRMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300
           SFAEALRMGSEVYH LKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY
Sbjct: 241 SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGY 300

Query: 301 TGKIKIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIED 360
           TGKIKIGMDVAASEFFTK+GKYDLNFKKQPNDGAHVHSAH LGELYKQF+KDFPIVSIED
Sbjct: 301 TGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSIED 360

Query: 361 PFDQDDWSSWSSLQSSVDIQLVGDDLLVTNPKRIAEAIHKKACNALLLKVNQIGSVTESI 420
           PFDQDDWSSW+SLQSSVDIQLVGDDLLVTNPKRIAE I KKACNALLLKVNQIGSVTESI
Sbjct: 361 PFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESI 420

Query: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480
           QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE
Sbjct: 421 QAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIE 480

Query: 481 EELGSVSYAGESFRSP 497
           EELGSV YAG SFRSP
Sbjct: 481 EELGSVRYAGASFRSP 496

[Close]

BLAST of MS002079 vs. ExPASy TrEMBL
Match: A0A6J1EV05 (Phosphopyruvate hydratase OS=Cucurbita moschata OX=3662 GN=LOC111437858 PE=3 SV=1)

HSP 1 Score: 875.5 bits (2261), Expect = 1.0e-250
Identity = 456/498 (91.57%), Postives = 468/498 (93.98%), Query Frame = 0


Query: 1   MASLSSPP--AMNLLHHTLLPSKSHSLIASIPSSSSSSSSSSSSNHLRSRAVRCSVAVAP 60
           MASL SPP    NLL+ +LLPSK  S I SIPSSSSS         LRS  VRCSVAVAP
Sbjct: 1   MASLPSPPTTTTNLLNKSLLPSKPQSPIISIPSSSSSKP-------LRSHGVRCSVAVAP 60

Query: 61  SAATKASKEYKLKSVKARQIIDSRGNPTVEVDLVTDDLYRSAVPSGASTGIYEALELRDG 120
           SA+TKAS E+KLKSVKARQIIDSRGNPTVEVDL+TDDLYRSAVPSGASTGIYEALELRDG
Sbjct: 61  SASTKASSEFKLKSVKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDG 120

Query: 121 DKSVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGV 180
           DK+VYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGV
Sbjct: 121 DKNVYGGKGVLTAVKNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGV 180

Query: 181 SLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVG 240
           SLSVCRAGAGAKGLPLYRHIQELS TKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVG
Sbjct: 181 SLSVCRAGAGAKGLPLYRHIQELSDTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVG 240

Query: 241 ATSFAEALRMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKA 300
           A SFAEALRMGSEVYH LKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLL DAIEKA
Sbjct: 241 AASFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLTDAIEKA 300

Query: 301 GYTGKIKIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSI 360
           GYTGKIKIGMDVAASEFFTKDG+YDLNFKKQPNDGAHVHSAHGLGELYKQF+ DFPIVSI
Sbjct: 301 GYTGKIKIGMDVAASEFFTKDGRYDLNFKKQPNDGAHVHSAHGLGELYKQFVNDFPIVSI 360

Query: 361 EDPFDQDDWSSWSSLQSSVDIQLVGDDLLVTNPKRIAEAIHKKACNALLLKVNQIGSVTE 420
           EDPFDQDDWSSW+SLQSSV +Q+VGDDLLVTNPKRIAEAI KKACNALLLKVNQIGSVTE
Sbjct: 361 EDPFDQDDWSSWASLQSSVGVQIVGDDLLVTNPKRIAEAIQKKACNALLLKVNQIGSVTE 420

Query: 421 SIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLR 480
           SIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLR
Sbjct: 421 SIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLR 480

Query: 481 IEEELGSVSYAGESFRSP 497
           IEEELG+V YAGESFRSP
Sbjct: 481 IEEELGNVRYAGESFRSP 491

[Close]

BLAST of MS002079 vs. TAIR 10
Match: AT1G74030.1 (enolase 1 )

HSP 1 Score: 781.2 bits (2016), Expect = 5.0e-226
Identity = 404/484 (83.47%), Postives = 434/484 (89.67%), Query Frame = 0


Query: 18  LPSKSHSLIASIPSSSSSS-----SSSSSSNHLRSRAVRCSVAVAPSAATKASKEYKLKS 77
           L +K H L  S  S S  S      S+ S    R   V+CSV          +KE ++K 
Sbjct: 3   LTTKPHHLQRSFLSPSRVSGERYLESAPSCLRFRRSGVQCSV---------VAKECRVKG 62

Query: 78  VKARQIIDSRGNPTVEVDLVTDDLYRSAVPSGASTGIYEALELRDGDKSVYGGKGVLTAV 137
           VKARQIIDSRGNPTVEVDL+TDDLYRSAVPSGASTGIYEALELRDGDKSVYGGKGVL A+
Sbjct: 63  VKARQIIDSRGNPTVEVDLITDDLYRSAVPSGASTGIYEALELRDGDKSVYGGKGVLQAI 122

Query: 138 KNINDILAPKLVGVDVRNQEDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRAGAGAKGL 197
           KNIN+++APKL+GVDVRNQ DVDA+MLE+DGT NKSKLGANAILGVSLSVCRAGAGAKG+
Sbjct: 123 KNINELVAPKLIGVDVRNQADVDALMLELDGTPNKSKLGANAILGVSLSVCRAGAGAKGV 182

Query: 198 PLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAEALRMGSEV 257
           PLY+HIQE SGTKELVMPVPAFNVINGGSHAGN+LAMQEFMILPVGATSF+EA +MGSEV
Sbjct: 183 PLYKHIQETSGTKELVMPVPAFNVINGGSHAGNSLAMQEFMILPVGATSFSEAFQMGSEV 242

Query: 258 YHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAA 317
           YH LKGIIK KYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAA
Sbjct: 243 YHTLKGIIKTKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAA 302

Query: 318 SEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIEDPFDQDDWSSWSS 377
           SEFF KDG+YDLNFKKQPNDGAHV SA  L +LY++FIKDFPIVSIEDPFDQDDWSSW+S
Sbjct: 303 SEFFMKDGRYDLNFKKQPNDGAHVLSAESLADLYREFIKDFPIVSIEDPFDQDDWSSWAS 362

Query: 378 LQSSVDIQLVGDDLLVTNPKRIAEAIHKKACNALLLKVNQIGSVTESIQAALDSKAAGWG 437
           LQSSVDIQLVGDDLLVTNPKRIAEAI K++CNALLLKVNQIG+VTESIQAALDSKAAGWG
Sbjct: 363 LQSSVDIQLVGDDLLVTNPKRIAEAIKKQSCNALLLKVNQIGTVTESIQAALDSKAAGWG 422

Query: 438 VMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGSVSYAGES 497
           VMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERL+KYNQLLRIEEELG+V YAGE+
Sbjct: 423 VMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLSKYNQLLRIEEELGNVRYAGEA 477

[Close]

BLAST of MS002079 vs. TAIR 10
Match: AT2G36530.1 (Enolase )

HSP 1 Score: 576.6 bits (1485), Expect = 1.9e-164
Identity = 298/435 (68.51%), Postives = 352/435 (80.92%), Query Frame = 0


Query: 73  VKARQIIDSRGNPTVEVDLVTDDLYR--SAVPSGASTGIYEALELRDGDKSVYGGKGVLT 132
           VKARQI DSRGNPTVEVD+ T +  +  +AVPSGASTGIYEALELRDG  S Y GKGV  
Sbjct: 7   VKARQIFDSRGNPTVEVDIHTSNGIKVTAAVPSGASTGIYEALELRDGG-SDYLGKGVSK 66

Query: 133 AVKNINDILAPKLVGVDVRNQEDVDAIML-EIDGTTN-----KSKLGANAILGVSLSVCR 192
           AV N+N+I+ P L+G D   Q  +D  M+ E+DGT N     K KLGANAIL VSL+VC+
Sbjct: 67  AVGNVNNIIGPALIGKDPTQQTAIDNFMVHELDGTQNEWGWCKQKLGANAILAVSLAVCK 126

Query: 193 AGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGATSFAE 252
           AGA   G+PLY+HI  L+G  ++V+PVPAFNVINGGSHAGN LAMQEFMILPVGA SF E
Sbjct: 127 AGAVVSGIPLYKHIANLAGNPKIVLPVPAFNVINGGSHAGNKLAMQEFMILPVGAASFKE 186

Query: 253 ALRMGSEVYHILKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKI 312
           A++MG EVYH LK +IK KYGQDA NVGDEGGFAPN+Q+N+EGL LL  AIEKAGYTGK+
Sbjct: 187 AMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAGYTGKV 246

Query: 313 KIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVHSAHGLGELYKQFIKDFPIVSIEDPFDQ 372
            IGMDVAASEF+++D  YDLNFK++ N+G+   S   L +LYK F+ ++PIVSIEDPFDQ
Sbjct: 247 VIGMDVAASEFYSEDKTYDLNFKEENNNGSQKISGDALKDLYKSFVAEYPIVSIEDPFDQ 306

Query: 373 DDWSSWSSL--QSSVDIQLVGDDLLVTNPKRIAEAIHKKACNALLLKVNQIGSVTESIQA 432
           DDW  ++ +  +   ++Q+VGDDLLVTNPKR+A+AI +K+CNALLLKVNQIGSVTESI+A
Sbjct: 307 DDWEHYAKMTTECGTEVQIVGDDLLVTNPKRVAKAIAEKSCNALLLKVNQIGSVTESIEA 366

Query: 433 ALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEE 492
              SK AGWGVM SHRSGETED FIADL+VGL++GQIKTGAPCRSERLAKYNQLLRIEEE
Sbjct: 367 VKMSKKAGWGVMTSHRSGETEDTFIADLAVGLSTGQIKTGAPCRSERLAKYNQLLRIEEE 426

Query: 493 LGSVS-YAGESFRSP 497
           LGS + YAG +FR P
Sbjct: 427 LGSEAIYAGVNFRKP 440

[Close]

BLAST of MS002079 vs. TAIR 10
Match: AT2G29560.1 (cytosolic enolase )

HSP 1 Score: 461.5 bits (1186), Expect = 8.8e-130
Identity = 241/464 (51.94%), Postives = 320/464 (68.97%), Query Frame = 0


Query: 44  HLRSRAVRCSVAVAPSAAT-----------KASKEYKLKSVKARQIIDSRGNPTVEVDLV 103
           H+ SR +  +V  A  A T           K +    +  VKARQI+DSRG PTVEVDL 
Sbjct: 10  HMLSRKIEDAVNAAVRAKTSDPVLFIANHLKKAVSSVITKVKARQILDSRGIPTVEVDLH 69

Query: 104 TD-DLYRSAVPSGASTGIYEALELRDGDKSVYGGKGVLTAVKNINDILAPKLVGVDVRNQ 163
           T+  ++R++VPSG S+G YEA+ELRDGDK +Y G  V  AVKNIN+ ++  L+G+D + Q
Sbjct: 70  TNKGVFRASVPSGDSSGTYEAIELRDGDKGMYLGNSVAKAVKNINEKISEALIGMDPKLQ 129

Query: 164 EDVDAIMLEIDGTTNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPV 223
             +D  M+++D T  KS+LGANAIL VS++ C+AGA  K +PL +H+ +LSG   +V+PV
Sbjct: 130 GQIDQAMIDLDKTEKKSELGANAILAVSIAACKAGAAEKEVPLCKHLSDLSGRANMVLPV 189

Query: 224 PAFNVINGGSHAGNNLAMQEFMILPVGATSFAEALRMGSEVYHILKGIIKAKYGQDACNV 283
           PAF V++GG HA N  A+QE MILP+GA+ F EAL+ GSE YH LK +I  K G   CNV
Sbjct: 190 PAFTVLSGGKHASNTFAIQEIMILPIGASRFEEALQWGSETYHHLKAVISEKNGGLGCNV 249

Query: 284 GDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKDGKYDLNFKKQPN 343
           G++GG AP++   +EGL L+ +AI + GY  KIKI +D+AA+  F    KYDL+ K    
Sbjct: 250 GEDGGLAPDISSLKEGLELVKEAINRTGYNDKIKIAIDIAATN-FCLGTKYDLDIKSPNK 309

Query: 344 DGAHVHSAHGLGELYKQFIKDFPIVSIEDPFDQDDWSSWSSLQSSVDIQLVGDDLLVTNP 403
            G +  SA  + ++YK+   D+PIVSIEDPFD++DW       S    Q+VGDDLL++N 
Sbjct: 310 SGQNFKSAEDMIDMYKEICNDYPIVSIEDPFDKEDWEHTKYFSSLGICQVVGDDLLMSNS 369

Query: 404 KRIAEAIHKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLS 463
           KR+  AI + +CNALLLKVNQIG+VTE+I+    ++ A WGV+ SHR GETED+FI+DLS
Sbjct: 370 KRVERAIQESSCNALLLKVNQIGTVTEAIEVVKMARDAQWGVVTSHRCGETEDSFISDLS 429

Query: 464 VGLASGQIKTGAPCRSERLAKYNQLLRIEEELGSVS-YAGESFR 495
           VGLA+G IK GAPCR ER  KYNQLLRIEEELG  + YAGE ++
Sbjct: 430 VGLATGVIKAGAPCRGERTMKYNQLLRIEEELGDQAVYAGEDWK 472

The following BLAST results are available for this feature:

BLAST of MS002079 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134829.1	1.4e-273	99.60	enolase 1, chloroplastic [Momordica charantia]	[more]
XP_038879680.1	3.5e-253	91.94	enolase 1, chloroplastic [Benincasa hispida]	[more]
TYK28380.1	4.5e-253	92.54	enolase 1 [Cucumis melo var. makuwa]	[more]
XP_008453338.1	1.0e-252	92.34	PREDICTED: enolase 1, chloroplastic [Cucumis melo] >KAA0058034.1 enolase 1 [Cucu...	[more]
KAG6587623.1	1.2e-250	91.57	Enolase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of MS002079 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C9C4	7.1e-225	83.47	Enolase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ENO1 PE=1 SV=1	[more]
P42894	2.5e-169	70.74	Enolase OS=Neocallimastix frontalis OX=4757 PE=3 SV=1	[more]
Q27527	4.2e-169	71.23	Enolase OS=Caenorhabditis elegans OX=6239 GN=enol-1 PE=1 SV=3	[more]
Q54RK5	7.2e-169	70.30	Enolase A OS=Dictyostelium discoideum OX=44689 GN=enoA PE=3 SV=1	[more]
P15429	1.2e-168	70.77	Beta-enolase OS=Rattus norvegicus OX=10116 GN=Eno3 PE=1 SV=3	[more]

BLAST of MS002079 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BZW1	6.5e-274	99.60	Phosphopyruvate hydratase OS=Momordica charantia OX=3673 GN=LOC111007005 PE=3 SV...	[more]
A0A5D3DYS6	2.2e-253	92.54	Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A5A7US84	4.9e-253	92.34	Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]
A0A1S3BX56	4.9e-253	92.34	Phosphopyruvate hydratase OS=Cucumis melo OX=3656 GN=LOC103494088 PE=3 SV=1	[more]
A0A6J1EV05	1.0e-250	91.57	Phosphopyruvate hydratase OS=Cucurbita moschata OX=3662 GN=LOC111437858 PE=3 SV=...	[more]

BLAST of MS002079 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G74030.1	5.0e-226	83.47	enolase 1	[more]
AT2G36530.1	1.9e-164	68.51	Enolase	[more]
AT2G29560.1	8.8e-130	51.94	cytosolic enolase	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000941	Enolase	PRINTS	PR00148	ENOLASE	coord: 228..241 score: 75.87 coord: 435..452 score: 83.0 coord: 406..420 score: 80.2 coord: 101..115 score: 88.38 coord: 383..394 score: 78.16 coord: 171..187 score: 76.74
IPR000941	Enolase	TIGRFAM	TIGR01060	TIGR01060	coord: 71..492 e-value: 1.4E-164 score: 545.7
IPR000941	Enolase	SFLD	SFLDF00002	enolase	coord: 70..483 e-value: 0.0 score: 621.2
IPR000941	Enolase	PANTHER	PTHR11902	ENOLASE	coord: 34..496
IPR000941	Enolase	HAMAP	MF_00318	Enolase	coord: 68..483 score: 43.350121
IPR000941	Enolase	CDD	cd03313	enolase	coord: 72..482 e-value: 0.0 score: 714.257
IPR020810	Enolase, C-terminal TIM barrel domain	SMART	SM01192	Enolase_C_3	coord: 206..496 e-value: 4.0E-190 score: 647.6
IPR020810	Enolase, C-terminal TIM barrel domain	PFAM	PF00113	Enolase_C	coord: 207..494 e-value: 5.9E-147 score: 488.6
IPR020811	Enolase, N-terminal	SMART	SM01193	Enolase_N_3	coord: 70..198 e-value: 2.3E-84 score: 296.3
IPR020811	Enolase, N-terminal	PFAM	PF03952	Enolase_N	coord: 71..198 e-value: 8.8E-56 score: 187.7
IPR029017	Enolase-like, N-terminal	GENE3D	3.30.390.10		coord: 64..191 e-value: 5.7E-56 score: 190.2
IPR029017	Enolase-like, N-terminal	SUPERFAMILY	54826	Enolase N-terminal domain-like	coord: 70..201
IPR036849	Enolase-like, C-terminal domain superfamily	GENE3D	3.20.20.120		coord: 192..496 e-value: 1.2E-151 score: 506.1
IPR036849	Enolase-like, C-terminal domain superfamily	SUPERFAMILY	51604	Enolase C-terminal domain-like	coord: 206..487
None	No IPR available	SFLD	SFLDG00178	enolase	coord: 70..483 e-value: 0.0 score: 621.2
None	No IPR available	PANTHER	PTHR11902:SF42	ENOLASE 1, CHLOROPLASTIC	coord: 34..496
IPR020809	Enolase, conserved site	PROSITE	PS00164	ENOLASE	coord: 406..419

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS002079.1	MS002079.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006096	glycolytic process
cellular_component	GO:0000015	phosphopyruvate hydratase complex
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0004634	phosphopyruvate hydratase activity