MS002077 (gene) Bitter gourd (TR) v1

Overview


Name	MS002077
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	protein MIS12 homolog
Location	scaffold30: 2229421 .. 2231914 (-)
RNA-Seq Expression	MS002077
Synteny	MS002077

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGGAAGTAAGGGCGAAGCGGTATTCGATTCACTGAATCTGAATCCACGGCTCTTCATTAATGAAGCTCTCAATCTTGTCGACGATTTGGTCGATGACGCTTTCGATTTTTATCAATCGTATTGCTCTCTGTCTCGCATCGTCTCTTCAATTTAGTTTTTCAGTTTTGTTCGGTTAATTTGATTCTTGCGAATTCTTGTTACCTTCAGACAAGCATCAGCCACCTTGAAGACCGAGGGCGTGGATAAATCCCAGGATCTTATCATGGTGAGGTTTGAGTTATATTTCTGTCTTCCGATTGGTTAAGTTGGTACTCTGAAGCATTAGTTTGGAATGGGCACAATTCTCTTTGAATAAAATTGTTTATTTACCTTGTTCGTGTAGGGCATATCGCGCGTCCGATCGTTCGTCCATTCAGTTTTGGATAAGCGGTTGGCCATGTGGGAGAAGTACTGTCTGAATCACTGTTTTGCGGTCCCTGAAGGCTTTTCTTTACCCTCAAATGTAGGTTCTTCTATTCTATCTGTGATGAGGCCCTTACACTTTGTTACGTGAATTCGTTCTATTATTGTTGTTTTTGTAATTGAGTTCGTTGCCCTTGCTGTTCGAATCACTTCATAAGTGATTTATATTGAGCATGTAAGACGGCTACTTGCTTATGCAGCGAGTTGCCCACTTATACTGACTTGAGAACTTCCTCAATTAATTTTTAAAAAAGGCTCTATTACTATTTCATCATTGAGTTTCAAACTTTTGCAACATAGATTCCTCATTTTGATTGCTATATCTTGACGTGGTTCAGTTGATACTGGTCGTGACTGTATAAGATTTTCTAACTCTAAAAGTATTGTAAACAAGTGCCTGCTGCATTTTTTAGAAAAACACTGTGGTGTATCATGCTCCTTTTTCTTTTTTTCCCTTTTTTTGTTTTGTCAATCATATCTGAATCCTAACCTCTGTGAGGTCTAGTTCGACTCCTTTTGTTGTGCATCAATATAGTTGCGTGGGTTAGCAGTGTTTCTGTCTGTTTCACTGGGATCTCATTCGAAGCCTTGACTATTGTCAAGTTGCTAAATTGTCGATGGTTCCCTTATCTTGTTTGAGAACTTTGAGTTATGGTTAATTGAGTCTATGTATTCTCTTATTATTTTTCTTCTTCCTATGCAACAAACCCATTTCTAATTCAGATCGAAATATTTTCCTTAAGTTGTCTTAGGATGAATCACCTGCCGACACTTCGATAAGTCATGATTGCCTCTATGACGTGGGTCTGGATACAGAGTTAGATTCTTTGAGGAACAGGCTCTCTGAGGTAATAATTGTGCCGCATGTTTTTTACTTCTCATGTTTCTGTACATAATATTTCATAAGAAAACATATTTTTCAGGTCAGAAAAGAGAATGTTGTATTGAACCAAGAACTCCAAGCTTTAGAAAGGCAAACTGCTTCCAGTAACTCACAAATCAATCATTTCAACGAGGCATTAGAATTATACGAGGAGACTTCAGTGAATGAGAGGTTCCAGGGTAATTAAGTTGTGCTTAAAAATCGTTGTTTGCTCGTGCACTGTTAGATATCTTATAAATAATTAAATTTACCCTAACCCTTCAAGTTAAACTTTTGGATTTATTGGTAGGTTAATCATGTTTAAAATGCACTCCTGGGCTTACATATGCATTATCCTAGTAGTATACTCATGAAGGCTGACCCATAATGTCATATGCAATTAACGTATGAAGCTGACTTATTTTATTTTTTCTTATAAGGAAGTTTCATTGTCAGAATTGGCTTGGTAGACTAAAATTTCTGTAAACACTTAACTTTCTGCTCTGAAGGATCTTATGGCCTGCATTTCTCCTTGTGACGCAAACTGTGATACATACAAAAGAATTTCAGTGAACATCTGCTAAATCGTTACGTTCTGGTTTCATTTGGGCAATTTTATTTTACATCAAGGAATAGATTTAGTGGATATTCCTTTTTGTTTCCCCTTTTCAATTGCTCATTGCGTAAAAATGGAACTTTAGTCTGTGAATTCTGTAGTTTCTCTAGCTATTTTCACTCTAGAGGTCACTCGGGACAAAACTTACTAATTTGCATAAATTTTCATTGTACCGAAATTTTAACAGTGGACAGATAAAACCTGTTCATGATTCATGGAGATAAAATTCTAACATATATCAAATTTCTGCAGAAGTGATGAACACTGCTTCGGAGCTCCGAGCGAAAATAGGAAAGCTGAAGAAAAGGAGGATAGAGGAATCCAAGCTCACTAAAGTAGAGAAGGTTCATACAAATGGCGACATGTCTCACCATCACAAGGGTAAGATTTTCAAGCTACTTCACGCATCGATCAAGTTGGTTTTAGCTTTGTGGGAAGGCGAATACAATATTGTTCCACATGATATTTGATGCTTGTTTCTGCTCTGCAGGCTTCTCTAATGCTAAGCTGGATGACATTCAAGAATTTTTAGCTGATTTGAAGACCCTT

mRNA sequence

ATGGAAGGAAGTAAGGGCGAAGCGGTATTCGATTCACTGAATCTGAATCCACGGCTCTTCATTAATGAAGCTCTCAATCTTGTCGACGATTTGGTCGATGACGCTTTCGATTTTTATCAATCACAAGCATCAGCCACCTTGAAGACCGAGGGCGTGGATAAATCCCAGGATCTTATCATGGGCATATCGCGCGTCCGATCGTTCGTCCATTCAGTTTTGGATAAGCGGTTGGCCATGTGGGAGAAGTACTGTCTGAATCACTGTTTTGCGGTCCCTGAAGGCTTTTCTTTACCCTCAAATGATGAATCACCTGCCGACACTTCGATAAGTCATGATTGCCTCTATGACGTGGGTCTGGATACAGAGTTAGATTCTTTGAGGAACAGGCTCTCTGAGGTCAGAAAAGAGAATGTTGTATTGAACCAAGAACTCCAAGCTTTAGAAAGGCAAACTGCTTCCAGTAACTCACAAATCAATCATTTCAACGAGGCATTAGAATTATACGAGGAGACTTCAGTGAATGAGAGGTTCCAGGAAGTGATGAACACTGCTTCGGAGCTCCGAGCGAAAATAGGAAAGCTGAAGAAAAGGAGGATAGAGGAATCCAAGCTCACTAAAGTAGAGAAGGTTCATACAAATGGCGACATGTCTCACCATCACAAGGGCTTCTCTAATGCTAAGCTGGATGACATTCAAGAATTTTTAGCTGATTTGAAGACCCTT

Coding sequence (CDS)

ATGGAAGGAAGTAAGGGCGAAGCGGTATTCGATTCACTGAATCTGAATCCACGGCTCTTCATTAATGAAGCTCTCAATCTTGTCGACGATTTGGTCGATGACGCTTTCGATTTTTATCAATCACAAGCATCAGCCACCTTGAAGACCGAGGGCGTGGATAAATCCCAGGATCTTATCATGGGCATATCGCGCGTCCGATCGTTCGTCCATTCAGTTTTGGATAAGCGGTTGGCCATGTGGGAGAAGTACTGTCTGAATCACTGTTTTGCGGTCCCTGAAGGCTTTTCTTTACCCTCAAATGATGAATCACCTGCCGACACTTCGATAAGTCATGATTGCCTCTATGACGTGGGTCTGGATACAGAGTTAGATTCTTTGAGGAACAGGCTCTCTGAGGTCAGAAAAGAGAATGTTGTATTGAACCAAGAACTCCAAGCTTTAGAAAGGCAAACTGCTTCCAGTAACTCACAAATCAATCATTTCAACGAGGCATTAGAATTATACGAGGAGACTTCAGTGAATGAGAGGTTCCAGGAAGTGATGAACACTGCTTCGGAGCTCCGAGCGAAAATAGGAAAGCTGAAGAAAAGGAGGATAGAGGAATCCAAGCTCACTAAAGTAGAGAAGGTTCATACAAATGGCGACATGTCTCACCATCACAAGGGCTTCTCTAATGCTAAGCTGGATGACATTCAAGAATTTTTAGCTGATTTGAAGACCCTT

Protein sequence

MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEVMNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKTL

Homology

[Close]

BLAST of MS002077 vs. NCBI nr
Match: XP_022134693.1 (protein MIS12 homolog [Momordica charantia])

HSP 1 Score: 464.9 bits (1195), Expect = 4.2e-127
Identity = 238/241 (98.76%), Postives = 240/241 (99.59%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIM 60
           MEGSKGEAVFDSLNLNP+LFINEALNLVDDLVDDAFDFYQ QASATLKTEGVDKSQDLIM
Sbjct: 1   MEGSKGEAVFDSLNLNPQLFINEALNLVDDLVDDAFDFYQLQASATLKTEGVDKSQDLIM 60

Query: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120
           GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD
Sbjct: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120

Query: 121 TELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180
           TELDSLRNRLSEVR+ENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV
Sbjct: 121 TELDSLRNRLSEVREENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180

Query: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT 240
           MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT
Sbjct: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT 240

Query: 241 L 242
           L
Sbjct: 241 L 241

[Close]

BLAST of MS002077 vs. NCBI nr
Match: KAG6589575.1 (Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 417.5 bits (1072), Expect = 7.6e-113
Identity = 210/241 (87.14%), Postives = 227/241 (94.19%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIM 60
           MEGSKGE VFDSLNLNP+LFINEALN+VDDLVDDAFDFYQ+QASA+LKTEG D+SQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120
           GISRVR+FV S LDKRLAMWEKYCLNHCF+VPEGFSLPSNDESP  TS+S DCLYDV LD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180
           TELD LRN+LSEVRKENVVLNQELQALERQTASSNSQ+N FNEAL+LYE+ SVNE FQE+
Sbjct: 121 TELDFLRNKLSEVRKENVVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQEM 180

Query: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT 240
           M TASELRAKIGKLKKRR+EESKLTKVEK+HTNGD+SHHHKGFSNAKLDDIQEFLADLK 
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDISHHHKGFSNAKLDDIQEFLADLKA 240

Query: 241 L 242
           L
Sbjct: 241 L 241

[Close]

BLAST of MS002077 vs. NCBI nr
Match: XP_023515499.1 (protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 417.2 bits (1071), Expect = 9.9e-113
Identity = 210/241 (87.14%), Postives = 227/241 (94.19%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIM 60
           MEGSKGE VFDSLNLNP+LFINEALN+VDDLVDDAFDFYQ+QASA+LKTEG D+SQDL +
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLSL 60

Query: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120
           GISRVR+FV S LDKRLAMWEKYCLNHCF+VPEGFSLPSNDESP  TS+S DCLYDV LD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180
           TELD LRN+LSEVRKENVVLNQELQALERQTASSNSQ+N FNEAL+LYE+ SVNE FQE+
Sbjct: 121 TELDFLRNKLSEVRKENVVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQEM 180

Query: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT 240
           M TASELRAKIGKLKKRR+EESKLTKVEK+HTNGD+SHHHKGFSNAKLDDIQEFLADLKT
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDISHHHKGFSNAKLDDIQEFLADLKT 240

Query: 241 L 242
           L
Sbjct: 241 L 241

[Close]

BLAST of MS002077 vs. NCBI nr
Match: KAG7023263.1 (Protein MIS12-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 414.8 bits (1065), Expect = 4.9e-112
Identity = 209/241 (86.72%), Postives = 225/241 (93.36%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIM 60
           MEGSKGE VFDSLNLNP+LFINEALN+VDDLVDDAFDFYQ+QASA+LKTEG D+SQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120
           GISRVR+FV S LDKRLAMWEKYCLNHCF+VPEGFSLPSNDESP  TS+S DCLYDV LD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180
           TELD LRN+LSEVRKENVVLNQELQALERQ ASSNSQ+N FNEAL+LYE  SVNE FQE+
Sbjct: 121 TELDFLRNKLSEVRKENVVLNQELQALERQAASSNSQVNRFNEALQLYERNSVNEMFQEM 180

Query: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT 240
           M TASELRAKIGKLKKRR+EESKLTKVEK+HTNGD+SHHHKGFSNAKLDDIQEFLADLK 
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDISHHHKGFSNAKLDDIQEFLADLKA 240

Query: 241 L 242
           L
Sbjct: 241 L 241

[Close]

BLAST of MS002077 vs. NCBI nr
Match: XP_022921372.1 (protein MIS12 homolog [Cucurbita moschata])

HSP 1 Score: 413.7 bits (1062), Expect = 1.1e-111
Identity = 208/241 (86.31%), Postives = 224/241 (92.95%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIM 60
           MEGSKGE VFDSLNLNP+LFINEALN+VDDLVDDAFDFYQ+QASA+LKTEG D+SQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120
           GISRVR+FV S LDKRLAMWEKYCLNHCF+VPEGFSLPSNDESP  TS+S DCLYDV LD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180
           TELD LRN+LSEVRKEN+VLNQELQALERQ ASSNSQ+N FNEAL+LYE  SVNE FQE+
Sbjct: 121 TELDFLRNKLSEVRKENIVLNQELQALERQAASSNSQVNRFNEALQLYERNSVNEMFQEM 180

Query: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT 240
           M TASELRAKIGKLKKRR+EESKLTKVEK+HTNGD SHHHKGFSNAKLDDIQEFLADLK 
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDASHHHKGFSNAKLDDIQEFLADLKA 240

Query: 241 L 242
           L
Sbjct: 241 L 241

[Close]

BLAST of MS002077 vs. ExPASy Swiss-Prot
Match: Q2V0Z5 (Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 2.8e-57
Identity = 126/247 (51.01%), Postives = 173/247 (70.04%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVD--KSQDL 60
           MEGSK EAVFDS+NLNP++FINEA+N V+D VD AFDFY   AS +LK +G D  KSQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  IMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVG 120
             GI+RVR  + SV+D RL +WE Y L  CFAVP+GF LP ++ES   +S+  D LYD+ 
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKSEES---SSVHQDGLYDLE 120

Query: 121 LDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQ 180
           LD ELDSLR++L+ V K +V L+ ELQALER + S    +   NEALELY+E+S++E F+
Sbjct: 121 LDAELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDEIFK 180

Query: 181 EVMNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFS----NAKLDDIQEF 240
           E+   ASELRA + +LK RR++ S+  KV++      + +H K FS    + KL+D+++F
Sbjct: 181 EMTKMASELRASVERLKTRRMKASESAKVKR------LKNHGKEFSAMTFDGKLEDLEKF 238

Query: 241 LADLKTL 242
            A+L+ +
Sbjct: 241 QAELRKM 238

[Close]

BLAST of MS002077 vs. ExPASy TrEMBL
Match: A0A6J1C2Q6 (protein MIS12 homolog OS=Momordica charantia OX=3673 GN=LOC111006900 PE=3 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 2.0e-127
Identity = 238/241 (98.76%), Postives = 240/241 (99.59%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIM 60
           MEGSKGEAVFDSLNLNP+LFINEALNLVDDLVDDAFDFYQ QASATLKTEGVDKSQDLIM
Sbjct: 1   MEGSKGEAVFDSLNLNPQLFINEALNLVDDLVDDAFDFYQLQASATLKTEGVDKSQDLIM 60

Query: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120
           GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD
Sbjct: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120

Query: 121 TELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180
           TELDSLRNRLSEVR+ENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV
Sbjct: 121 TELDSLRNRLSEVREENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180

Query: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT 240
           MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT
Sbjct: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT 240

Query: 241 L 242
           L
Sbjct: 241 L 241

[Close]

BLAST of MS002077 vs. ExPASy TrEMBL
Match: A0A6J1E0A1 (protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 5.3e-112
Identity = 208/241 (86.31%), Postives = 224/241 (92.95%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIM 60
           MEGSKGE VFDSLNLNP+LFINEALN+VDDLVDDAFDFYQ+QASA+LKTEG D+SQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120
           GISRVR+FV S LDKRLAMWEKYCLNHCF+VPEGFSLPSNDESP  TS+S DCLYDV LD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180
           TELD LRN+LSEVRKEN+VLNQELQALERQ ASSNSQ+N FNEAL+LYE  SVNE FQE+
Sbjct: 121 TELDFLRNKLSEVRKENIVLNQELQALERQAASSNSQVNRFNEALQLYERNSVNEMFQEM 180

Query: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT 240
           M TASELRAKIGKLKKRR+EESKLTKVEK+HTNGD SHHHKGFSNAKLDDIQEFLADLK 
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGDASHHHKGFSNAKLDDIQEFLADLKA 240

Query: 241 L 242
           L
Sbjct: 241 L 241

[Close]

BLAST of MS002077 vs. ExPASy TrEMBL
Match: A0A6J1JME3 (protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 9.1e-112
Identity = 208/241 (86.31%), Postives = 225/241 (93.36%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIM 60
           MEGSKGE VFDSLNLNP+LFINEALN+VDDLVDDAFDFYQ+QASA+LKTEG D+SQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120
           GISRVR+FV S LDKRLAMWEKYCLNHCF+VPEGFSLPSNDESP  TS+S DCLYDV LD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180
           TELD LR  LSEVRKEN+VLNQELQALERQTASSNSQ+N FNEAL+LYE+ SVNE FQE+
Sbjct: 121 TELDFLRKNLSEVRKENIVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQEM 180

Query: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT 240
           M TASELRAKIGKLKKRR+EESKLTKVEK+HTN D+SHHHKGFSNAKLDDIQEFLADLKT
Sbjct: 181 MGTASELRAKIGKLKKRRMEESKLTKVEKIHTNRDISHHHKGFSNAKLDDIQEFLADLKT 240

Query: 241 L 242
           L
Sbjct: 241 L 241

[Close]

BLAST of MS002077 vs. ExPASy TrEMBL
Match: A0A6J1JD43 (protein MIS12 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 2.2e-110
Identity = 208/242 (85.95%), Postives = 225/242 (92.98%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIM 60
           MEGSKGE VFDSLNLNP+LFINEALN+VDDLVDDAFDFYQ+QASA+LKTEG D+SQDLI+
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120
           GISRVR+FV S LDKRLAMWEKYCLNHCF+VPEGFSLPSNDESP  TS+S DCLYDV LD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQ-E 180
           TELD LR  LSEVRKEN+VLNQELQALERQTASSNSQ+N FNEAL+LYE+ SVNE FQ E
Sbjct: 121 TELDFLRKNLSEVRKENIVLNQELQALERQTASSNSQVNRFNEALQLYEQNSVNEMFQAE 180

Query: 181 VMNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLK 240
           +M TASELRAKIGKLKKRR+EESKLTKVEK+HTN D+SHHHKGFSNAKLDDIQEFLADLK
Sbjct: 181 MMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNRDISHHHKGFSNAKLDDIQEFLADLK 240

Query: 241 TL 242
           TL
Sbjct: 241 TL 242

[Close]

BLAST of MS002077 vs. ExPASy TrEMBL
Match: A0A5A7UQ95 (Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003370 PE=3 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 2.4e-104
Identity = 197/241 (81.74%), Postives = 221/241 (91.70%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVDKSQDLIM 60
           MEGSKGE+VF+SLNLNP+LFINEALN VDDLVDDAFDFYQSQASATLKTEG D+SQDL +
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASATLKTEGSDRSQDLTL 60

Query: 61  GISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVGLD 120
           GIS+VR+ V   LDKRLAMWEKYCLNHCF+VPEGFSLPSNDESP  TSI+HD   DV LD
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHD--QDVDLD 120

Query: 121 TELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQEV 180
           TELD LRN+LSEVRKEN+VLNQELQALERQTASSNSQI+HFNEAL+LYE++SVN+ FQE+
Sbjct: 121 TELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEM 180

Query: 181 MNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFSNAKLDDIQEFLADLKT 240
           + TASELR+KIGK+KKRR EE+KL KVEKVHTNGD+SHHH GFSNAKLDDIQEFL+DLK 
Sbjct: 181 IKTASELRSKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKK 239

Query: 241 L 242
           +
Sbjct: 241 I 239

[Close]

BLAST of MS002077 vs. TAIR 10
Match: AT5G35520.1 (minichromosome instability 12 (mis12)-like )

HSP 1 Score: 223.4 bits (568), Expect = 2.0e-58
Identity = 126/247 (51.01%), Postives = 173/247 (70.04%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPRLFINEALNLVDDLVDDAFDFYQSQASATLKTEGVD--KSQDL 60
           MEGSK EAVFDS+NLNP++FINEA+N V+D VD AFDFY   AS +LK +G D  KSQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  IMGISRVRSFVHSVLDKRLAMWEKYCLNHCFAVPEGFSLPSNDESPADTSISHDCLYDVG 120
             GI+RVR  + SV+D RL +WE Y L  CFAVP+GF LP ++ES   +S+  D LYD+ 
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKSEES---SSVHQDGLYDLE 120

Query: 121 LDTELDSLRNRLSEVRKENVVLNQELQALERQTASSNSQINHFNEALELYEETSVNERFQ 180
           LD ELDSLR++L+ V K +V L+ ELQALER + S    +   NEALELY+E+S++E F+
Sbjct: 121 LDAELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDEIFK 180

Query: 181 EVMNTASELRAKIGKLKKRRIEESKLTKVEKVHTNGDMSHHHKGFS----NAKLDDIQEF 240
           E+   ASELRA + +LK RR++ S+  KV++      + +H K FS    + KL+D+++F
Sbjct: 181 EMTKMASELRASVERLKTRRMKASESAKVKR------LKNHGKEFSAMTFDGKLEDLEKF 238

Query: 241 LADLKTL 242
            A+L+ +
Sbjct: 241 QAELRKM 238

The following BLAST results are available for this feature:

BLAST of MS002077 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134693.1	4.2e-127	98.76	protein MIS12 homolog [Momordica charantia]	[more]
KAG6589575.1	7.6e-113	87.14	Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023515499.1	9.9e-113	87.14	protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo]	[more]
KAG7023263.1	4.9e-112	86.72	Protein MIS12-like protein [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022921372.1	1.1e-111	86.31	protein MIS12 homolog [Cucurbita moschata]	[more]

BLAST of MS002077 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2V0Z5	2.8e-57	51.01	Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1	[more]

BLAST of MS002077 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C2Q6	2.0e-127	98.76	protein MIS12 homolog OS=Momordica charantia OX=3673 GN=LOC111006900 PE=3 SV=1	[more]
A0A6J1E0A1	5.3e-112	86.31	protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1	[more]
A0A6J1JME3	9.1e-112	86.31	protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=...	[more]
A0A6J1JD43	2.2e-110	85.95	protein MIS12 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=...	[more]
A0A5A7UQ95	2.4e-104	81.74	Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol...	[more]

BLAST of MS002077 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35520.1	2.0e-58	51.01	minichromosome instability 12 (mis12)-like	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 123..157
None	No IPR available	COILS	Coil	Coil	coord: 177..197
IPR008685	Centromere protein Mis12	PFAM	PF05859	Mis12	coord: 11..141 e-value: 1.3E-18 score: 67.4
IPR008685	Centromere protein Mis12	PANTHER	PTHR14527	PROTEIN MIS12 HOMOLOG	coord: 1..240

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS002077.1	MS002077.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000278	mitotic cell cycle
cellular_component	GO:0000775	chromosome, centromeric region
cellular_component	GO:0005634	nucleus