MS002054 (gene) Bitter gourd (TR) v1

BLAST of MS002054 vs. NCBI nr
Match: XP_022135054.1 (uncharacterized protein LOC111007140 isoform X1 [Momordica charantia] >XP_022135056.1 uncharacterized protein LOC111007140 isoform X1 [Momordica charantia])

HSP 1 Score: 2418.7 bits (6267), Expect = 0.0e+00
Identity = 1214/1253 (96.89%), Postives = 1219/1253 (97.29%), Query Frame = 0

Query: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKAMNIFSL 60
            MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSK       
Sbjct: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSK------- 60

Query: 61   SRSPSIRGSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSIS 120
                   GSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSIS
Sbjct: 61   -------GSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSIS 120

Query: 121  QFADSAWSDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAK 180
            QFADSAWSDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAK
Sbjct: 121  QFADSAWSDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAK 180

Query: 181  GKKGFVFNDLKGNDDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQ 240
            GKKGFVFNDLKGNDDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQ
Sbjct: 181  GKKGFVFNDLKGNDDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQ 240

Query: 241  ANSRGANKKVAHRTYFSSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDL 300
            ANSRGANKKV HRTY  SCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDL
Sbjct: 241  ANSRGANKKVPHRTYSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDL 300

Query: 301  LQNHGITICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYLYEQNGRWNDGNTG-- 360
            LQNHGITICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYL EQNGRWNDGNTG  
Sbjct: 301  LQNHGITICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGKH 360

Query: 361  ------KDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGG 420
                  KDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGG
Sbjct: 361  VNRDAPKDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGG 420

Query: 421  EEKRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANY 480
            EEKRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANY
Sbjct: 421  EEKRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANY 480

Query: 481  VEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLE 540
            VEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLE
Sbjct: 481  VEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLE 540

Query: 541  SMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNAR 600
            SMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNAR
Sbjct: 541  SMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNAR 600

Query: 601  LLITNPDMLHVSILPYHRQFSRILSNLRFVIIDEAHTYKGAFGCHTALILRRLRRICSHV 660
            LLITNPDMLHVSILPYHRQFSRILSNLRFV+IDEAHTYKGAFGCHTALILRRLRRICSHV
Sbjct: 601  LLITNPDMLHVSILPYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHV 660

Query: 661  YGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRG 720
            YGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQR 
Sbjct: 661  YGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRD 720

Query: 721  IDSLQSTEKNVNFRNPRQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTRE 780
            IDSLQSTEKNVNFRNP      PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTRE
Sbjct: 721  IDSLQSTEKNVNFRNPS-----PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTRE 780

Query: 781  ILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLH 840
            ILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLH
Sbjct: 781  ILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLH 840

Query: 841  LGFPGSIASLWQQAGRAGRREKTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGN 900
            LGFPGSIASLWQQAGRAGRRE+TSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGN
Sbjct: 841  LGFPGSIASLWQQAGRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGN 900

Query: 901  QQVLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIG 960
            QQVLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIG
Sbjct: 901  QQVLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIG 960

Query: 961  QEKMPSRAVSIRAIETERYKVIDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSL 1020
            QEKMPSRAVSIRAIETERY+V+DQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSL
Sbjct: 961  QEKMPSRAVSIRAIETERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSL 1020

Query: 1021 NLSSMLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTW 1080
            NLSSMLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTW
Sbjct: 1021 NLSSMLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTW 1080

Query: 1081 FGFYRIWKGSKQIFDTVDLSLPKYSYNSQQAVWIPVPQSVKEEVKRKSFDFRAGLHAASH 1140
            FGFYRIWKGSKQIFDTVDLSLPKYSYNS QAVWIPVPQSVKEEVKRKSFDFRAGLHAASH
Sbjct: 1081 FGFYRIWKGSKQIFDTVDLSLPKYSYNS-QAVWIPVPQSVKEEVKRKSFDFRAGLHAASH 1140

Query: 1141 ALLNVVPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNA 1200
            ALLNVVPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNA
Sbjct: 1141 ALLNVVPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNA 1200

Query: 1201 ALELLTSCCCLGETGCPNCVQSLACHEYNELLHKDAASLIIKGVLDAEKSYCR 1246
            ALELLTSCCCLGETGCPNCVQSLACHEYNE+LHKDAASLIIKGVLDAEKSYCR
Sbjct: 1201 ALELLTSCCCLGETGCPNCVQSLACHEYNEVLHKDAASLIIKGVLDAEKSYCR 1233

BLAST of MS002054 vs. NCBI nr
Match: XP_022135057.1 (uncharacterized protein LOC111007140 isoform X2 [Momordica charantia])

HSP 1 Score: 2287.3 bits (5926), Expect = 0.0e+00
Identity = 1162/1253 (92.74%), Postives = 1167/1253 (93.14%), Query Frame = 0

Query: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKAMNIFSL 60
            MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSK       
Sbjct: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSK------- 60

Query: 61   SRSPSIRGSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSIS 120
                   GSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSIS
Sbjct: 61   -------GSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSIS 120

Query: 121  QFADSAWSDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAK 180
            QFADSAWSDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAK
Sbjct: 121  QFADSAWSDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAK 180

Query: 181  GKKGFVFNDLKGNDDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQ 240
            GKKGFVFNDLKGNDDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQ
Sbjct: 181  GKKGFVFNDLKGNDDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQ 240

Query: 241  ANSRGANKKVAHRTYFSSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDL 300
            ANSRGANKKV HRTY  SCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDL
Sbjct: 241  ANSRGANKKVPHRTYSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDL 300

Query: 301  LQNHGITICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYLYEQNGRWNDGNTG-- 360
            LQNHGITICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYL EQNGRWNDGNTG  
Sbjct: 301  LQNHGITICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGKH 360

Query: 361  ------KDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGG 420
                  KDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGG
Sbjct: 361  VNRDAPKDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGG 420

Query: 421  EEKRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANY 480
            EEKRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANY
Sbjct: 421  EEKRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANY 480

Query: 481  VEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLE 540
            VEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLE
Sbjct: 481  VEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLE 540

Query: 541  SMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNAR 600
            SMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNAR
Sbjct: 541  SMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNAR 600

Query: 601  LLITNPDMLHVSILPYHRQFSRILSNLRFVIIDEAHTYKGAFGCHTALILRRLRRICSHV 660
            LLITNPDMLHVSILPYHRQFSRILSNLRFV+IDEAHTYKGAFGCHTALILRRLRRICSHV
Sbjct: 601  LLITNPDMLHVSILPYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHV 660

Query: 661  YGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRG 720
            YGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQR 
Sbjct: 661  YGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRD 720

Query: 721  IDSLQSTEKNVNFRNPRQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTRE 780
            IDSLQSTEKNVNFRNP      PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTRE
Sbjct: 721  IDSLQSTEKNVNFRNPS-----PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTRE 780

Query: 781  ILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLH 840
            ILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLH
Sbjct: 781  ILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLH 840

Query: 841  LGFPGSIASLWQQAGRAGRREKTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGN 900
            LGFPGSIASLWQQAGRAGRRE+TSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGN
Sbjct: 841  LGFPGSIASLWQQAGRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGN 900

Query: 901  QQVLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIG 960
            QQVLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIG
Sbjct: 901  QQVLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIG 960

Query: 961  QEKMPSRAVSIRAIETERYKVIDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSL 1020
            QEKMPSRAVSIRAIETERY+V+DQRQNEVIEEIEESKAFFQ                   
Sbjct: 961  QEKMPSRAVSIRAIETERYRVVDQRQNEVIEEIEESKAFFQ------------------- 1020

Query: 1021 NLSSMLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTW 1080
                                             AYPRRFPNIPPSKTTAQANDCRVTTTW
Sbjct: 1021 ---------------------------------AYPRRFPNIPPSKTTAQANDCRVTTTW 1080

Query: 1081 FGFYRIWKGSKQIFDTVDLSLPKYSYNSQQAVWIPVPQSVKEEVKRKSFDFRAGLHAASH 1140
            FGFYRIWKGSKQIFDTVDLSLPKYSYNS QAVWIPVPQSVKEEVKRKSFDFRAGLHAASH
Sbjct: 1081 FGFYRIWKGSKQIFDTVDLSLPKYSYNS-QAVWIPVPQSVKEEVKRKSFDFRAGLHAASH 1140

Query: 1141 ALLNVVPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNA 1200
            ALLNVVPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNA
Sbjct: 1141 ALLNVVPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNA 1181

Query: 1201 ALELLTSCCCLGETGCPNCVQSLACHEYNELLHKDAASLIIKGVLDAEKSYCR 1246
            ALELLTSCCCLGETGCPNCVQSLACHEYNE+LHKDAASLIIKGVLDAEKSYCR
Sbjct: 1201 ALELLTSCCCLGETGCPNCVQSLACHEYNEVLHKDAASLIIKGVLDAEKSYCR 1181

BLAST of MS002054 vs. NCBI nr
Match: XP_022135058.1 (uncharacterized protein LOC111007140 isoform X3 [Momordica charantia])

HSP 1 Score: 2197.2 bits (5692), Expect = 0.0e+00
Identity = 1110/1149 (96.61%), Postives = 1115/1149 (97.04%), Query Frame = 0

Query: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKAMNIFSL 60
            MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSK       
Sbjct: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSK------- 60

Query: 61   SRSPSIRGSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSIS 120
                   GSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSIS
Sbjct: 61   -------GSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSIS 120

Query: 121  QFADSAWSDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAK 180
            QFADSAWSDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAK
Sbjct: 121  QFADSAWSDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAK 180

Query: 181  GKKGFVFNDLKGNDDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQ 240
            GKKGFVFNDLKGNDDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQ
Sbjct: 181  GKKGFVFNDLKGNDDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQ 240

Query: 241  ANSRGANKKVAHRTYFSSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDL 300
            ANSRGANKKV HRTY  SCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDL
Sbjct: 241  ANSRGANKKVPHRTYSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDL 300

Query: 301  LQNHGITICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYLYEQNGRWNDGNTG-- 360
            LQNHGITICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYL EQNGRWNDGNTG  
Sbjct: 301  LQNHGITICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGKH 360

Query: 361  ------KDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGG 420
                  KDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGG
Sbjct: 361  VNRDAPKDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGG 420

Query: 421  EEKRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANY 480
            EEKRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANY
Sbjct: 421  EEKRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANY 480

Query: 481  VEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLE 540
            VEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLE
Sbjct: 481  VEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLE 540

Query: 541  SMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNAR 600
            SMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNAR
Sbjct: 541  SMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNAR 600

Query: 601  LLITNPDMLHVSILPYHRQFSRILSNLRFVIIDEAHTYKGAFGCHTALILRRLRRICSHV 660
            LLITNPDMLHVSILPYHRQFSRILSNLRFV+IDEAHTYKGAFGCHTALILRRLRRICSHV
Sbjct: 601  LLITNPDMLHVSILPYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHV 660

Query: 661  YGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRG 720
            YGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQR 
Sbjct: 661  YGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRD 720

Query: 721  IDSLQSTEKNVNFRNPRQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTRE 780
            IDSLQSTEKNVNFRNP      PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTRE
Sbjct: 721  IDSLQSTEKNVNFRNPS-----PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTRE 780

Query: 781  ILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLH 840
            ILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLH
Sbjct: 781  ILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLH 840

Query: 841  LGFPGSIASLWQQAGRAGRREKTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGN 900
            LGFPGSIASLWQQAGRAGRRE+TSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGN
Sbjct: 841  LGFPGSIASLWQQAGRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGN 900

Query: 901  QQVLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIG 960
            QQVLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIG
Sbjct: 901  QQVLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIG 960

Query: 961  QEKMPSRAVSIRAIETERYKVIDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSL 1020
            QEKMPSRAVSIRAIETERY+V+DQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSL
Sbjct: 961  QEKMPSRAVSIRAIETERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSL 1020

Query: 1021 NLSSMLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTW 1080
            NLSSMLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTW
Sbjct: 1021 NLSSMLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTW 1080

Query: 1081 FGFYRIWKGSKQIFDTVDLSLPKYSYNSQQAVWIPVPQSVKEEVKRKSFDFRAGLHAASH 1140
            FGFYRIWKGSKQIFDTVDLSLPKYSYNS QAVWIPVPQSVKEEVKRKSFDFRAGLHAASH
Sbjct: 1081 FGFYRIWKGSKQIFDTVDLSLPKYSYNS-QAVWIPVPQSVKEEVKRKSFDFRAGLHAASH 1129

Query: 1141 ALLNVVPLR 1142
            ALLNVVPL+
Sbjct: 1141 ALLNVVPLK 1129

BLAST of MS002054 vs. NCBI nr
Match: XP_038878313.1 (uncharacterized ATP-dependent helicase YprA isoform X7 [Benincasa hispida])

HSP 1 Score: 2103.2 bits (5448), Expect = 0.0e+00
Identity = 1060/1249 (84.87%), Postives = 1131/1249 (90.55%), Query Frame = 0

Query: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKAMNIFSL 60
            MEES REI I+TLTGESLTV ISG+RTIDDLKLLLRRNFPSAT SPNFHLF K       
Sbjct: 1    MEESVREIEIKTLTGESLTVSISGNRTIDDLKLLLRRNFPSATISPNFHLFFK------- 60

Query: 61   SRSPSIRGSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSIS 120
                   G+KLKPQS I ACRIDHGEFLVLIPFNKKESSKS+LRDQYEQ SSVS  SSIS
Sbjct: 61   -------GTKLKPQSQISACRIDHGEFLVLIPFNKKESSKSRLRDQYEQGSSVSCGSSIS 120

Query: 121  QFADSAWSDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAK 180
            QFADSAWSDMVQDLSYLH CS + R+EN  E ERG+SE+G V+AELV T S+SS SSK+K
Sbjct: 121  QFADSAWSDMVQDLSYLHGCSDERREENRPESERGSSEVGGVDAELVATCSTSSFSSKSK 180

Query: 181  GKKGFVFNDLKGN-DDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAK 240
            GKKGF ++DLKGN DDVLR   SS +EGFLNE TCE+F+K+LESVDCLSDPRNG CMLAK
Sbjct: 181  GKKGFAYDDLKGNLDDVLRKFSSSPSEGFLNEQTCESFIKYLESVDCLSDPRNGNCMLAK 240

Query: 241  QANSRGANKKVAHRTYFSSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMD 300
            QANSR  NK+  + T  SSCLCPVWLKK  KAFSFLNVF+M LQLQE+IMTVSRLEQAMD
Sbjct: 241  QANSRSGNKRAPNSTCGSSCLCPVWLKKTMKAFSFLNVFAMFLQLQEEIMTVSRLEQAMD 300

Query: 301  LLQNHGITICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYLYEQNGRWND--GNT 360
             L+  GIT+C EDI++LSLLCPKAVHFASG  ED+C D L I+IYL E+NGRW D   NT
Sbjct: 301  QLRKRGITLCMEDIQNLSLLCPKAVHFASGRSEDTCVDRLIIIIYLTEKNGRWKDDIDNT 360

Query: 361  G-KDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEEKR 420
               +VT LKRRERSFKFYL EAIK HML+HGSR EICVPFSLE LITP E+ V G E KR
Sbjct: 361  APTNVTPLKRRERSFKFYLLEAIKCHMLKHGSRREICVPFSLEALITPNETDVDGSEAKR 420

Query: 421  RKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIP 480
             KK DTA SSS+SD+ QCHDTSKLLPE MVEHL+ GVGS+GQIVHVEDI ARKANYVEIP
Sbjct: 421  GKKCDTA-SSSQSDRRQCHDTSKLLPEKMVEHLEKGVGSEGQIVHVEDIGARKANYVEIP 480

Query: 481  EELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQ 540
            +ELSNNV+SALKCIGV  LYSHQA+SIEASLAGKHVAVATMTSSGKSLCYNLPVLE+MSQ
Sbjct: 481  KELSNNVVSALKCIGVANLYSHQAQSIEASLAGKHVAVATMTSSGKSLCYNLPVLEAMSQ 540

Query: 541  NVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLIT 600
            NV+SCALYLFPTKALAQDQLRSLL+MMKGF+ NLNIGVYDGDTSQADR+LLRDNARLLIT
Sbjct: 541  NVSSCALYLFPTKALAQDQLRSLLIMMKGFNHNLNIGVYDGDTSQADRILLRDNARLLIT 600

Query: 601  NPDMLHVSILPYHRQFSRILSNLRFVIIDEAHTYKGAFGCHTALILRRLRRICSHVYGSD 660
            NPDMLHVSILP+HRQFSRILSNLRF++IDEAHTYKGAFGCHTALILRRLRR+CSHVYGSD
Sbjct: 601  NPDMLHVSILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALILRRLRRLCSHVYGSD 660

Query: 661  PSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRGIDSL 720
            PSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPI ALKNYQRGIDS 
Sbjct: 661  PSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIMALKNYQRGIDSP 720

Query: 721  QSTEKNVNFRNPRQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKE 780
            QST+KN+  RNP      PIMDIARLFAEMV HGLRCIAFCKTRKLCELVLCYTREILKE
Sbjct: 721  QSTKKNMTVRNPS-----PIMDIARLFAEMVLHGLRCIAFCKTRKLCELVLCYTREILKE 780

Query: 781  RAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP 840
             APHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP
Sbjct: 781  SAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFP 840

Query: 841  GSIASLWQQAGRAGRREKTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVL 900
            GSIASLWQQAGRAGRREKTSLSVYVAF+GPLDQYF+K+PEKLFGSPIECCHIDA NQQVL
Sbjct: 841  GSIASLWQQAGRAGRREKTSLSVYVAFEGPLDQYFVKHPEKLFGSPIECCHIDAKNQQVL 900

Query: 901  EQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKM 960
            EQHLLCAA+EHP+ V YDQNFFG GLNTAL SLKNRGDLI + SCGSSKSIWNYIGQEKM
Sbjct: 901  EQHLLCAAYEHPVCVAYDQNFFGPGLNTALMSLKNRGDLIAESSCGSSKSIWNYIGQEKM 960

Query: 961  PSRAVSIRAIETERYKVIDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSS 1020
            PSR+VSIRAIE ERYKV+DQRQNE++EEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLS+
Sbjct: 961  PSRSVSIRAIEAERYKVVDQRQNEILEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLST 1020

Query: 1021 MLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFY 1080
            MLAFCEEADLKYYTKTRDYTDIHVI GN+AYPRR PNIPPSKTTAQANDCRVTTTWFGFY
Sbjct: 1021 MLAFCEEADLKYYTKTRDYTDIHVIDGNLAYPRRAPNIPPSKTTAQANDCRVTTTWFGFY 1080

Query: 1081 RIWKGSKQIFDTVDLSLPKYSYNSQQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLN 1140
            +I KGS QI DT++LSLPKYSYNS QAVW+PVPQS+KEEVKRK+FDFRAGLHAASHALLN
Sbjct: 1081 QIQKGSNQIIDTLNLSLPKYSYNS-QAVWVPVPQSIKEEVKRKNFDFRAGLHAASHALLN 1140

Query: 1141 VVPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNAALEL 1200
            VVPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTG+S+QIQPVFIELLNAA EL
Sbjct: 1141 VVPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTGLSLQIQPVFIELLNAAFEL 1200

Query: 1201 LTSCCCLGETGCPNCVQSLACHEYNELLHKDAASLIIKGVLDAEKSYCR 1246
            LTSC CLGETGCPNCVQSL CHEYNE+LHKDAASLIIKGVLDAEK+YCR
Sbjct: 1201 LTSCRCLGETGCPNCVQSLTCHEYNEVLHKDAASLIIKGVLDAEKAYCR 1228

BLAST of MS002054 vs. NCBI nr
Match: XP_038878311.1 (uncharacterized ATP-dependent helicase YprA isoform X5 [Benincasa hispida])

HSP 1 Score: 2097.0 bits (5432), Expect = 0.0e+00
Identity = 1058/1263 (83.77%), Postives = 1129/1263 (89.39%), Query Frame = 0

Query: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKAMNIFSL 60
            MEES REI I+TLTGESLTV ISG+RTIDDLKLLLRRNFPSAT SPNFHLF K       
Sbjct: 1    MEESVREIEIKTLTGESLTVSISGNRTIDDLKLLLRRNFPSATISPNFHLFFK------- 60

Query: 61   SRSPSIRGSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSIS 120
                   G+KLKPQS I ACRIDHGEFLVLIPFNKKESSKS+LRDQYEQ SSVS  SSIS
Sbjct: 61   -------GTKLKPQSQISACRIDHGEFLVLIPFNKKESSKSRLRDQYEQGSSVSCGSSIS 120

Query: 121  QFADSAWSDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAK 180
            QFADSAWSDMVQDLSYLH CS + R+EN  E ERG+SE+G V+AELV T S+SS SSK+K
Sbjct: 121  QFADSAWSDMVQDLSYLHGCSDERREENRPESERGSSEVGGVDAELVATCSTSSFSSKSK 180

Query: 181  GKKGFVFNDLKGN-DDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAK 240
            GKKGF ++DLKGN DDVLR   SS +EGFLNE TCE+F+K+LESVDCLSDPRNG CMLAK
Sbjct: 181  GKKGFAYDDLKGNLDDVLRKFSSSPSEGFLNEQTCESFIKYLESVDCLSDPRNGNCMLAK 240

Query: 241  QANSRGANKKVAHRTYFSSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMD 300
            QANSR  NK+  + T  SSCLCPVWLKK  KAFSFLNVF+M LQLQE+IMTVSRLEQAMD
Sbjct: 241  QANSRSGNKRAPNSTCGSSCLCPVWLKKTMKAFSFLNVFAMFLQLQEEIMTVSRLEQAMD 300

Query: 301  LLQNHGITICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYLYEQNGRWNDG---- 360
             L+  GIT+C EDI++LSLLCPKAVHFASG  ED+C D L I+IYL E+NGRW D     
Sbjct: 301  QLRKRGITLCMEDIQNLSLLCPKAVHFASGRSEDTCVDRLIIIIYLTEKNGRWKDDIDNT 360

Query: 361  -------------NTGKDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLI 420
                             +VT LKRRERSFKFYL EAIK HML+HGSR EICVPFSLE LI
Sbjct: 361  VTSHRCCTSCKSFTAPTNVTPLKRRERSFKFYLLEAIKCHMLKHGSRREICVPFSLEALI 420

Query: 421  TPKESSVHGGEEKRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHV 480
            TP E+ V G E KR KK DTA SSS+SD+ QCHDTSKLLPE MVEHL+ GVGS+GQIVHV
Sbjct: 421  TPNETDVDGSEAKRGKKCDTA-SSSQSDRRQCHDTSKLLPEKMVEHLEKGVGSEGQIVHV 480

Query: 481  EDIAARKANYVEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGK 540
            EDI ARKANYVEIP+ELSNNV+SALKCIGV  LYSHQA+SIEASLAGKHVAVATMTSSGK
Sbjct: 481  EDIGARKANYVEIPKELSNNVVSALKCIGVANLYSHQAQSIEASLAGKHVAVATMTSSGK 540

Query: 541  SLCYNLPVLESMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQA 600
            SLCYNLPVLE+MSQNV+SCALYLFPTKALAQDQLRSLL+MMKGF+ NLNIGVYDGDTSQA
Sbjct: 541  SLCYNLPVLEAMSQNVSSCALYLFPTKALAQDQLRSLLIMMKGFNHNLNIGVYDGDTSQA 600

Query: 601  DRVLLRDNARLLITNPDMLHVSILPYHRQFSRILSNLRFVIIDEAHTYKGAFGCHTALIL 660
            DR+LLRDNARLLITNPDMLHVSILP+HRQFSRILSNLRF++IDEAHTYKGAFGCHTALIL
Sbjct: 601  DRILLRDNARLLITNPDMLHVSILPHHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALIL 660

Query: 661  RRLRRICSHVYGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNP 720
            RRLRR+CSHVYGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNP
Sbjct: 661  RRLRRLCSHVYGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNP 720

Query: 721  IKALKNYQRGIDSLQSTEKNVNFRNPRQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKL 780
            I ALKNYQRGIDS QST+KN+  RNP      PIMDIARLFAEMV HGLRCIAFCKTRKL
Sbjct: 721  IMALKNYQRGIDSPQSTKKNMTVRNPS-----PIMDIARLFAEMVLHGLRCIAFCKTRKL 780

Query: 781  CELVLCYTREILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGI 840
            CELVLCYTREILKE APHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGI
Sbjct: 781  CELVLCYTREILKESAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGI 840

Query: 841  DVGHIDATLHLGFPGSIASLWQQAGRAGRREKTSLSVYVAFDGPLDQYFMKNPEKLFGSP 900
            DVGHIDATLHLGFPGSIASLWQQAGRAGRREKTSLSVYVAF+GPLDQYF+K+PEKLFGSP
Sbjct: 841  DVGHIDATLHLGFPGSIASLWQQAGRAGRREKTSLSVYVAFEGPLDQYFVKHPEKLFGSP 900

Query: 901  IECCHIDAGNQQVLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCG 960
            IECCHIDA NQQVLEQHLLCAA+EHP+ V YDQNFFG GLNTAL SLKNRGDLI + SCG
Sbjct: 901  IECCHIDAKNQQVLEQHLLCAAYEHPVCVAYDQNFFGPGLNTALMSLKNRGDLIAESSCG 960

Query: 961  SSKSIWNYIGQEKMPSRAVSIRAIETERYKVIDQRQNEVIEEIEESKAFFQVYEGAVYMH 1020
            SSKSIWNYIGQEKMPSR+VSIRAIE ERYKV+DQRQNE++EEIEESKAFFQVYEGAVYMH
Sbjct: 961  SSKSIWNYIGQEKMPSRSVSIRAIEAERYKVVDQRQNEILEEIEESKAFFQVYEGAVYMH 1020

Query: 1021 QGRTYLVKSLNLSSMLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQ 1080
            QGRTYLVKSLNLS+MLAFCEEADLKYYTKTRDYTDIHVI GN+AYPRR PNIPPSKTTAQ
Sbjct: 1021 QGRTYLVKSLNLSTMLAFCEEADLKYYTKTRDYTDIHVIDGNLAYPRRAPNIPPSKTTAQ 1080

Query: 1081 ANDCRVTTTWFGFYRIWKGSKQIFDTVDLSLPKYSYNSQQAVWIPVPQSVKEEVKRKSFD 1140
            ANDCRVTTTWFGFY+I KGS QI DT++LSLPKYSYNS QAVW+PVPQS+KEEVKRK+FD
Sbjct: 1081 ANDCRVTTTWFGFYQIQKGSNQIIDTLNLSLPKYSYNS-QAVWVPVPQSIKEEVKRKNFD 1140

Query: 1141 FRAGLHAASHALLNVVPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTGMSVQI 1200
            FRAGLHAASHALLNVVPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTG+S+QI
Sbjct: 1141 FRAGLHAASHALLNVVPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTGLSLQI 1200

Query: 1201 QPVFIELLNAALELLTSCCCLGETGCPNCVQSLACHEYNELLHKDAASLIIKGVLDAEKS 1246
            QPVFIELLNAA ELLTSC CLGETGCPNCVQSL CHEYNE+LHKDAASLIIKGVLDAEK+
Sbjct: 1201 QPVFIELLNAAFELLTSCRCLGETGCPNCVQSLTCHEYNEVLHKDAASLIIKGVLDAEKA 1242

BLAST of MS002054 vs. ExPASy Swiss-Prot
Match: Q05549 (ATP-dependent helicase HRQ1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HRQ1 PE=1 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 8.2e-129
Identity = 281/813 (34.56%), Postives = 435/813 (53.51%), Query Frame = 0

Query: 445  MVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVISALKCIGVEKLYSHQARSIE 504
            M+E LK+      QI H   I +R A Y  +  EL+  V   ++    E  YSHQA +I 
Sbjct: 244  MIEKLKSTEFYASQIKHCFTIPSRTAKYKGLCFELAPEVYQGME---HENFYSHQADAIN 303

Query: 505  ASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLFPTKALAQDQLRSLLVMMK 564
            +   G++V + T TSSGKSL Y L  ++ + ++  S  +Y+FPTKALAQDQ R+  V++ 
Sbjct: 304  SLHQGENVIITTSTSSGKSLIYQLAAIDLLLKDPESTFMYIFPTKALAQDQKRAFKVILS 363

Query: 565  GFSD--NLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSILPYHRQFSRILSNLRFV 624
               +  N  +  YDGDT   +R  +R NAR++ TNPDM+H SILP H  +   L +L+ V
Sbjct: 364  KIPELKNAVVDTYDGDTEPEERAYIRKNARVIFTNPDMIHTSILPNHANWRHFLYHLKLV 423

Query: 625  IIDEAHTYKGAFGCHTALILRRLRRICSHVY-GSDPSFIFCTATSANPREHCMELGNLSS 684
            ++DE H YKG FG H AL++RRL R+C   Y  S   FI C+AT  +P +H  ++  ++ 
Sbjct: 424  VVDELHIYKGLFGSHVALVMRRLLRLCHCFYENSGLQFISCSATLKSPVQHMKDMFGINE 483

Query: 685  LELIENDGSPSARKLFILWNPIKALKNYQRGIDSLQSTEKNVNFRNPRQQLSIPIMDIAR 744
            + LI  DGSP+  K  ++WNP   L  ++R         K  NF          I + A+
Sbjct: 484  VTLIHEDGSPTGAKHLVVWNP-PILPQHER---------KRENF----------IRESAK 543

Query: 745  LFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERA-PHLVQSVCAYRAGYTAEDRRRI 804
            +  +++ + +R IAFC  R++CEL++   R I  E     LV  V +YR GY+A DRR+I
Sbjct: 544  ILVQLILNNVRTIAFCYVRRVCELLMKEVRNIFIETGREDLVTEVMSYRGGYSASDRRKI 603

Query: 805  ESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQAGRAGRREKTSLSVY 864
            E + F GNL  V +TNALELGID+G +DA L  GFP S+A+  QQ+GRAGRR   SL++ 
Sbjct: 604  EREMFHGNLKAVISTNALELGIDIGGLDAVLMCGFPLSMANFHQQSGRAGRRNNDSLTLV 663

Query: 865  VAFDGPLDQYFMKNPEKLFG----SPIECCHIDAGNQQVLEQHLLCAAHEHPISVLYDQN 924
            VA D P+DQ+++ +PE L         +   +D  N  +LE H+ CAA E PI+   D+ 
Sbjct: 664  VASDSPVDQHYVAHPESLLEVNNFESYQDLVLDFNNILILEGHIQCAAFELPINFERDKQ 723

Query: 925  FF-GSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAIETERYKVID 984
            +F  S L        +      D    S++ +         PS+ VS+R  E +++ V+D
Sbjct: 724  YFTESHLRKICVERLHHNQ---DGYHASNRFL-------PWPSKCVSLRGGEEDQFAVVD 783

Query: 985  --QRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFCEEADLKYYTKTR 1044
                +N +IEEIE S+  F +Y+G +++HQG  YLVK  N     A  +  D+ + T  R
Sbjct: 784  ITNGRNIIIEEIEASRTSFTLYDGGIFIHQGYPYLVKEFNPDERYAKVQRVDVDWVTNQR 843

Query: 1045 DYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKGSKQIFDTVDLSL 1104
            D+TD+      +    R  ++P           + T   FGF+++ K  K+I D ++   
Sbjct: 844  DFTDVDPQEIELIRSLRNSDVP-----VYFGKIKTTIIVFGFFKVDK-YKRIIDAIETHN 903

Query: 1105 PKYSYNSQQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLRIICNMSDLAPEC 1164
            P    NS + +WI +P+   E  ++K  +    +H A HA++ ++P  I+  + ++  EC
Sbjct: 904  PPVIINS-KGLWIDMPKYALEICQKKQLNVAGAIHGAQHAIMGMLPRFIVAGVDEIQTEC 963

Query: 1165 ANP-------HDTRYFPERILLYDQHPG--GTGMSVQIQPVFIELLNAALELLTSCCCLG 1224
              P          R  P R++ YD   G  G+G+ V+      +++ ++L  +  C C  
Sbjct: 964  KAPEKEFAERQTKRKRPARLIFYDSKGGKYGSGLCVKAFEHIDDIIESSLRRIEECPC-- 1014

Query: 1225 ETGCPNCVQSLACHEYNELLHKDAASLIIKGVL 1238
              GCP+CV +  C E + +L K  A +++  +L
Sbjct: 1024 SDGCPDCVAASFCKENSLVLSKPGAQVVLHCIL 1014

BLAST of MS002054 vs. ExPASy Swiss-Prot
Match: O13983 (ATP-dependent helicase hrq1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=hrq1 PE=1 SV=4)

HSP 1 Score: 449.9 bits (1156), Expect = 9.4e-125
Identity = 272/836 (32.54%), Postives = 446/836 (53.35%), Query Frame = 0

Query: 424  SSSSKSDQIQ-CH-DTSKLLPEIMVEHLKAGVGSDGQIVH--VEDIAARKANYVEIPEEL 483
            S S  +D I+ C+ +T     E ++  + +    +GQIV   +    A +A Y  +   L
Sbjct: 236  SPSIPNDSIENCNLNTKACSIEELLNEIASESSYEGQIVQEALHTYPAVEAQYGALSRPL 295

Query: 484  SNNVISAL-KCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNV 543
            S  +I+AL     +EK Y HQA +I     G HV V+T TSSGKSL Y +P+L+S+ ++ 
Sbjct: 296  SQELINALYTSRNIEKTYKHQADAINHLWNGFHVIVSTSTSSGKSLIYQIPILQSLLEDN 355

Query: 544  TSCALYLFPTKALAQDQLRSLLVMMKGFS--DNLNIGVYDGDTSQADRVLLRDNARLLIT 603
             S A ++FPTK+LAQDQ +SL+ ++       N+ +  +DGDT    R  +  +A ++ T
Sbjct: 356  QSTAFFVFPTKSLAQDQKKSLIDILSYMPTLKNIRVDTFDGDTPLESRESIIRSANIIFT 415

Query: 604  NPDMLHVSILPYHRQFSRILSNLRFVIIDEAHTYKGAFGCHTALILRRLRRICSHVYGSD 663
            NPDMLH +ILP   ++     NL+  ++DEAH Y G FG H A +LRR+RRI  +   S 
Sbjct: 416  NPDMLHQTILPNANRWYYFFKNLKLFVLDEAHVYNGIFGVHVAFVLRRMRRIAEYFGNSQ 475

Query: 664  PSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRGIDSL 723
              F+ C+AT  +P +H  ++  + +++LI    SPS  K F++WNP              
Sbjct: 476  YRFVSCSATIEDPLQHMKKIFGVDNIKLINYTSSPSGSKKFVMWNP-------------- 535

Query: 724  QSTEKNVNFRNPRQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILK- 783
                  V+ ++P       I + ++L  +  +  +R I FC+ RK CE ++   R+ LK 
Sbjct: 536  ----PYVDPKHPDDGKKSAISEASKLLIKFAEKRVRTIVFCRVRKTCESLMRLVRQELKT 595

Query: 784  ERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGF 843
            ++   L+  + +YRAGYT ++RR+IES+ F G L G+ ATNALELGID+G +DA + +GF
Sbjct: 596  KQKGDLLSKIQSYRAGYTVQERRKIESEMFNGKLYGIIATNALELGIDIGSLDAVITIGF 655

Query: 844  PGSIASLWQQAGRAGRREKTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQV 903
            P S+++L QQ GRAGRR K+SL+VY+    P+DQ+++K+P  +   P     +D  N+ +
Sbjct: 656  PYSLSNLRQQFGRAGRRNKSSLAVYIVETFPVDQFYLKHPILIHTQPNAELTLDLTNEVL 715

Query: 904  LEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEK 963
            L  HL CAA+E PI++  D+ FFG+ +     +     +++ +      K +        
Sbjct: 716  LASHLQCAAYELPINIRSDEKFFGNQIQDICEA---NLEMVEESYRPHPKYL-------P 775

Query: 964  MPSRAVSIRAIETERYKVID--QRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLN 1023
             P+  V IR++  + + ++D    +N ++E +E  +     YEGAVY++QG+T++++ LN
Sbjct: 776  FPASQVRIRSVSEDMFTLVDVTNDKNVILELLEPFRVALTAYEGAVYVYQGKTFIIRLLN 835

Query: 1024 LSSMLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWF 1083
            ++  +    + D+++ T  RD+TD+  +   M             T       + T   F
Sbjct: 836  INKRIITAHQVDVEWSTLQRDFTDVDPVRSLMKKTMH------GSTNIYFGAVKATLHVF 895

Query: 1084 GFYRIWKGSKQIFDTVDLSLPKYSYNSQQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHA 1143
            G++++ K  K I D VD++      +S +  WI VP  + E +  K  +  A +HAA HA
Sbjct: 896  GYFKVNK-QKDILDVVDITDHPVEIDS-RGFWIDVPWHIIEVLSLKKINGAASIHAAQHA 955

Query: 1144 LLNVVPLRIICNMSDLAPEC-------ANPHDTRYFPERILLYDQ--HPGGTGMSVQIQP 1203
            LL+++P+ I  + +D+  EC             R  P R++ YD      G G+  +   
Sbjct: 956  LLSLMPIFISNSGNDIRTECKAGEKEYKEAKSERRRPSRLIFYDNCGDSSGAGLCNKAYE 1015

Query: 1204 VFIELLNAALELLTSCCCLGETGCPNCVQSLACHE---YNELLHKDAASLIIKGVL 1238
               EL+  A+E + SC C    GCP C+ S          E+L K  A +++K +L
Sbjct: 1016 HTDELITMAIERIESCDCKVREGCPGCITSSKFEGGVCSGEVLDKVGALILLKMLL 1035

BLAST of MS002054 vs. ExPASy Swiss-Prot
Match: P50830 (Uncharacterized ATP-dependent helicase YprA OS=Bacillus subtilis (strain 168) OX=224308 GN=yprA PE=1 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 4.4e-114
Identity = 248/757 (32.76%), Postives = 407/757 (53.76%), Query Frame = 0

Query: 459  IVHVEDIAARKANYVEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMT 518
            +V+  +I  R+A    +PE +   + +AL   G+++LY+HQ  + +    G+ +   T T
Sbjct: 19   VVNWHEIEPREAKTRPMPESIDERIKAALSKRGIDELYTHQYSAFQYVQKGESIVTVTPT 78

Query: 519  SSGKSLCYNLPVLESMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGD 578
            +SGK+LCYNLPVL+S++Q+ T+ ALYLFPTKALAQDQ   L  ++     ++    YDGD
Sbjct: 79   ASGKTLCYNLPVLQSIAQDETNRALYLFPTKALAQDQKSELNEIIDEMGIDIKSFTYDGD 138

Query: 579  TSQADRVLLRDNARLLITNPDMLHVSILPYHRQFSRILSNLRFVIIDEAHTYKGAFGCHT 638
            TS A R  +R    ++ITNPDMLH +ILP+H ++  +  NL++++IDE HTY+G FG H 
Sbjct: 139  TSPAIRQKVRKAGHIVITNPDMLHSAILPHHTKWVSLFENLKYIVIDELHTYRGVFGSHV 198

Query: 639  ALILRRLRRICSHVYGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFI 698
            A ++RRL+RIC   YGSDP FI  +AT ANP+E   +L     + L++++G+PS RK F+
Sbjct: 199  ANVIRRLKRIC-RFYGSDPVFICTSATIANPKELGEQLTG-KPMRLVDDNGAPSGRKHFV 258

Query: 699  LWNPIKALKNYQRGIDSLQSTEKNVNFRNPRQQLSIPIMDIARLFAEMVQHGLRCIAFCK 758
             +NP                  K +N R           ++  L  E +++ ++ I F +
Sbjct: 259  FYNP--------------PIVNKPLNIRRS------ATAEVNELAKEFLKNKVQTIVFAR 318

Query: 759  TRKLCELVLCYTREILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNAL 818
            +R   E++L + +E++K+      +S+  YR GY  ++RR IE     G++ GV +TNAL
Sbjct: 319  SRVRVEIILSHIQELVKKEIG--TKSIRGYRGGYLPKERREIERGLREGDILGVVSTNAL 378

Query: 819  ELGIDVGHIDATLHLGFPGSIASLWQQAGRAGRREKTSLSVYVAFDGPLDQYFMKNPEKL 878
            ELG+D+G +   +  G+PGS+AS WQQAGRAGRR   SL + VA   P+DQY +++PE  
Sbjct: 379  ELGVDIGQLQVCVMTGYPGSVASAWQQAGRAGRRHGESLIIMVANSTPIDQYIVRHPEYF 438

Query: 879  FGSPIECCHIDAGNQQVLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPD 938
            F    E   I+  N  +L  HL CAA+E P     D+ F    ++  L  L+    L   
Sbjct: 439  FNRSPESARINPENLIILVDHLKCAAYELPFRA--DEEFGAMEVSDILEYLQEEAVL--- 498

Query: 939  PSCGSSKSIWNYIGQEKMPSRAVSIRAIETERYKVIDQ---RQNEVIEEIEESKAFFQVY 998
                  +  W     E  P+  +S+R+   E   ++DQ       +I E++   A   ++
Sbjct: 499  -HRNGERYHW---ASESFPASNISLRSASQENVVIVDQSDIANVRIIGEMDRFSAMTLLH 558

Query: 999  EGAVYMHQGRTYLVKSLNLSSMLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIP 1058
            + A+Y+H+G  Y V+ L+     A+  + D++YYT       + V+  +    +      
Sbjct: 559  DEAIYLHEGVQYQVEKLDWDHKKAYVRKVDVEYYTDANLAVQLKVLEIDKTKEK------ 618

Query: 1059 PSKTTAQANDCRVTTTWFGFYRIWKGSKQIFDTVDLSLPKYSYNSQQAVWIPVPQSVKEE 1118
             S+T+    D  V      F +I   + +   +  + LP+   ++  A W+ + ++  E+
Sbjct: 619  -SRTSLHYGDVTVNALPTIFKKIKMTTFENIGSGPIHLPEEELHT-SAAWLEI-KTADED 678

Query: 1119 VKRKSFDFRAGLHAASHALLNVVPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGG 1178
            +  K+ +    L   S+ L ++VP+ I+C+ +D+             P  I LYD +PGG
Sbjct: 679  IGEKTLE--QLLLGISNVLQHIVPVYIMCDRNDVHVVSQIKAAHTGLP-TIFLYDHYPGG 728

Query: 1179 TGMSVQIQPVFIELLNAALELLTSCCCLGETGCPNCV 1213
             G++ ++   F ++  AA +L+T C C    GCP+C+
Sbjct: 739  IGLAEEVFKRFSDINEAAKQLITHCPC--HDGCPSCI 728

BLAST of MS002054 vs. ExPASy Swiss-Prot
Match: Q58969 (Uncharacterized ATP-dependent helicase MJ1574 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ1574 PE=3 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 2.4e-43
Identity = 155/593 (26.14%), Postives = 268/593 (45.19%), Query Frame = 0

Query: 454  GSDGQIVHVEDIAARKANY--VEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKH 513
            G + +I+ +  I  RK  +   +   +  N +I AL      KLY HQ ++++     K 
Sbjct: 7    GIEKEIIKIYKIPERKGRFSNFKFKNKEINELIDALGF----KLYLHQVKALKYLYNKKD 66

Query: 514  VAVATMTSSGKSLCYNLPVLESMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGF----S 573
            V V T T+SGKS  + L + ++   N     L ++PT+AL  +Q     +  + F    +
Sbjct: 67   VVVTTSTASGKSEIFRLAIFDNFLSNPDDRYLLIYPTRALINNQYEKFSMENELFYKITN 126

Query: 574  DNLNIGVYDGDTS-QADRVLLRDNARLLITNPDMLHVSILPYHRQFSRILSNLRFVIIDE 633
              +   +  GD   +  R +L+D   +L T PDMLH  IL  H  +  +L NL+ +++DE
Sbjct: 127  KRVKAEILTGDVGLEKRREILKDKPNVLFTTPDMLHYQILKNHNNYLWLLKNLKLLVVDE 186

Query: 634  AHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATSANPREHCMELGNLSSLELIE 693
             H Y+G FG +   + +RL ++   +  ++   +  +AT  NP+E    L N    E+++
Sbjct: 187  LHVYRGVFGTNMVYVFKRLLKLLKRL-NNNLQILCLSATLKNPKEFVKLLFN-RDFEVVD 246

Query: 694  NDGSPSARKLFILWNPIKALKNYQRGIDSLQSTEKNVNFRNPRQQLSIPIMDIARLFAEM 753
               +PS+RK   +  P K L N Q                            + RL   +
Sbjct: 247  KSYNPSSRKYLAILEP-KNLDNKQL---------------------------LRRLIENL 306

Query: 754  VQHGLRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFG 813
            V + ++ + F  TRK  E ++   R +L  +   +   +  Y+       R  IE  F  
Sbjct: 307  VDNNIKTLVFFDTRKETEKLM---RFLLNSK---VFYKLSTYKGTLPKYVREEIEEKFKN 366

Query: 814  GNLCGVAATNALELGIDVGHIDATLHLGF-PGSIASLWQQAGRAGRREKTSLSVYVAFDG 873
            G +  +  TNALELGID+G +DA ++ G  P  I SL Q+ GRAGRR+K +L++ V    
Sbjct: 367  GEILALLTTNALELGIDIGDLDAVINYGIPPDGIFSLIQRFGRAGRRDKEALNIIVLRKD 426

Query: 874  PLDQYFMKNPEKLF----GSPIECCHIDAGNQQVLEQHLLCAAHEHPISVLYDQNFFGSG 933
             LD Y+ ++  +L+       IE   ++  N+ V ++HL     E  I    + N F   
Sbjct: 427  GLDYYYKEHLNELYERIRKGIIEYMPVNIKNRFVTKKHLHYLISELKIVDFDELNDFEKE 486

Query: 934  LNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAVSIRAIETERY---------- 993
            +   +  L+  G +    +  ++K+    I   K P  + SIR    E Y          
Sbjct: 487  I---VKELEREGKIKIYKNPITNKT---EIRNVKQPIYS-SIRTASDESYYLILDKPWIK 546

Query: 994  -KVIDQRQNE-------------VIEEIEESKAFFQVYEGAVYMHQGRTYLVK 1011
             K++++ Q+E             VIEE+++ + +  +  G  Y  +G+ ++ K
Sbjct: 547  SKLLNKTQSEILSFINWLKIKGYVIEEVDKDEYYRSLITGMPYFSRGKLFIAK 552

BLAST of MS002054 vs. ExPASy Swiss-Prot
Match: Q57742 (Uncharacterized ATP-dependent helicase MJ0294 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0294 PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 4.1e-19
Identity = 129/541 (23.84%), Postives = 230/541 (42.51%), Query Frame = 0

Query: 499  QARSIEASLAGKHVAVATMTSSGKSLC-----YNLPVLESMSQNVTS--CALYLFPTKAL 558
            Q  +I+    GK+V + + T SGK+L       N  +  SM   +      LY+ P +AL
Sbjct: 39   QRYAIKEIHEGKNVLICSPTGSGKTLSAFLAGINELIKLSMENKLEDRIYILYVSPLRAL 98

Query: 559  AQDQLRSLLVMMKGF----------SDNLNIGVYDGDTSQADRV-LLRDNARLLITNPDM 618
              D  R+L   +K             D + + V   DT+ + +  +L+    +LIT P+ 
Sbjct: 99   NNDIERNLKEPLKEIYDVAKEIGIELDEIRVAVRTSDTTSSQKQRMLKKPPHILITTPES 158

Query: 619  LHVSILPYHRQFSRILSNLRFVIIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFI 678
            L +++     +FS++LS +++VI+DE H      G H +L L RL RI + +       I
Sbjct: 159  LAIAL--NSPKFSQLLSGIKYVIVDEIHALTNKRGVHLSLSLERLNRIANFIR------I 218

Query: 679  FCTATSANPREHCMEL-GNLSSLELIENDGSPSARKLFILWNPIKALKNYQRGIDSLQST 738
              +AT     E    L GN     +++                     +Y++ I+    +
Sbjct: 219  GLSATIHPLTEVAKFLVGNGRDCYIVD--------------------VSYKKEIEIKVIS 278

Query: 739  EKNVNFRNPRQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAP 798
              +     P +++S     +  L  ++++     + F  TR   E V  Y +++      
Sbjct: 279  PVDDFIYTPSEEIS---KRLYNLLKKLIEEHKTTLIFTNTRSATERVAFYLKQL------ 338

Query: 799  HLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSI 858
              V+ V  + +  + E R  +E     G +    ++ +LELG+D+G ID  + LG P S+
Sbjct: 339  -GVEKVETHHSSLSREHRLEVEEKLKKGEIRVCISSTSLELGVDIGSIDLVILLGSPKSV 398

Query: 859  ASLWQQAGRAGRR-EKTSLSVYVAFDGP--LDQYFMKNPEKLFGSPIECCHIDAGNQQVL 918
            +   Q+ GR+G R  + S  + + FD    ++   +    K+    I+  HI      VL
Sbjct: 399  SRALQRIGRSGHRLHEKSKGIIIPFDRDDLVENVVLAYDAKI--GKIDRIHIPKNCLDVL 458

Query: 919  EQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCG-SSKSIWN--YIGQ 978
             QHL+  A E    V    N           S K+  D++   + G   K+++   ++  
Sbjct: 459  AQHLVGMALEKVWDVDEAYNLIKKAYPYKDLSKKDFLDVLNYLAGGIEEKNVYAKIWLKD 518

Query: 979  EKMPSRAVSIRAIETERYKVI-DQRQNEVIE------EIEESKAFFQVYEGAVYMHQGRT 1008
             K   R  S+RAI       I D+   +VI       E+EE  A  ++ +G +++  G+T
Sbjct: 519  NKFGKRGKSVRAIYYMNVGTIPDETAVDVIADGKYVGEVEEEFA-EKLMKGDIFVLGGKT 538

BLAST of MS002054 vs. ExPASy TrEMBL
Match: A0A6J1BZJ1 (uncharacterized protein LOC111007140 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007140 PE=4 SV=1)

HSP 1 Score: 2418.7 bits (6267), Expect = 0.0e+00
Identity = 1214/1253 (96.89%), Postives = 1219/1253 (97.29%), Query Frame = 0

Query: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKAMNIFSL 60
            MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSK       
Sbjct: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSK------- 60

Query: 61   SRSPSIRGSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSIS 120
                   GSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSIS
Sbjct: 61   -------GSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSIS 120

Query: 121  QFADSAWSDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAK 180
            QFADSAWSDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAK
Sbjct: 121  QFADSAWSDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAK 180

Query: 181  GKKGFVFNDLKGNDDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQ 240
            GKKGFVFNDLKGNDDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQ
Sbjct: 181  GKKGFVFNDLKGNDDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQ 240

Query: 241  ANSRGANKKVAHRTYFSSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDL 300
            ANSRGANKKV HRTY  SCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDL
Sbjct: 241  ANSRGANKKVPHRTYSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDL 300

Query: 301  LQNHGITICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYLYEQNGRWNDGNTG-- 360
            LQNHGITICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYL EQNGRWNDGNTG  
Sbjct: 301  LQNHGITICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGKH 360

Query: 361  ------KDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGG 420
                  KDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGG
Sbjct: 361  VNRDAPKDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGG 420

Query: 421  EEKRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANY 480
            EEKRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANY
Sbjct: 421  EEKRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANY 480

Query: 481  VEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLE 540
            VEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLE
Sbjct: 481  VEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLE 540

Query: 541  SMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNAR 600
            SMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNAR
Sbjct: 541  SMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNAR 600

Query: 601  LLITNPDMLHVSILPYHRQFSRILSNLRFVIIDEAHTYKGAFGCHTALILRRLRRICSHV 660
            LLITNPDMLHVSILPYHRQFSRILSNLRFV+IDEAHTYKGAFGCHTALILRRLRRICSHV
Sbjct: 601  LLITNPDMLHVSILPYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHV 660

Query: 661  YGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRG 720
            YGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQR 
Sbjct: 661  YGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRD 720

Query: 721  IDSLQSTEKNVNFRNPRQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTRE 780
            IDSLQSTEKNVNFRNP      PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTRE
Sbjct: 721  IDSLQSTEKNVNFRNPS-----PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTRE 780

Query: 781  ILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLH 840
            ILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLH
Sbjct: 781  ILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLH 840

Query: 841  LGFPGSIASLWQQAGRAGRREKTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGN 900
            LGFPGSIASLWQQAGRAGRRE+TSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGN
Sbjct: 841  LGFPGSIASLWQQAGRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGN 900

Query: 901  QQVLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIG 960
            QQVLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIG
Sbjct: 901  QQVLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIG 960

Query: 961  QEKMPSRAVSIRAIETERYKVIDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSL 1020
            QEKMPSRAVSIRAIETERY+V+DQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSL
Sbjct: 961  QEKMPSRAVSIRAIETERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSL 1020

Query: 1021 NLSSMLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTW 1080
            NLSSMLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTW
Sbjct: 1021 NLSSMLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTW 1080

Query: 1081 FGFYRIWKGSKQIFDTVDLSLPKYSYNSQQAVWIPVPQSVKEEVKRKSFDFRAGLHAASH 1140
            FGFYRIWKGSKQIFDTVDLSLPKYSYNS QAVWIPVPQSVKEEVKRKSFDFRAGLHAASH
Sbjct: 1081 FGFYRIWKGSKQIFDTVDLSLPKYSYNS-QAVWIPVPQSVKEEVKRKSFDFRAGLHAASH 1140

Query: 1141 ALLNVVPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNA 1200
            ALLNVVPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNA
Sbjct: 1141 ALLNVVPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNA 1200

Query: 1201 ALELLTSCCCLGETGCPNCVQSLACHEYNELLHKDAASLIIKGVLDAEKSYCR 1246
            ALELLTSCCCLGETGCPNCVQSLACHEYNE+LHKDAASLIIKGVLDAEKSYCR
Sbjct: 1201 ALELLTSCCCLGETGCPNCVQSLACHEYNEVLHKDAASLIIKGVLDAEKSYCR 1233

BLAST of MS002054 vs. ExPASy TrEMBL
Match: A0A6J1C1K3 (uncharacterized protein LOC111007140 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007140 PE=4 SV=1)

HSP 1 Score: 2287.3 bits (5926), Expect = 0.0e+00
Identity = 1162/1253 (92.74%), Postives = 1167/1253 (93.14%), Query Frame = 0

Query: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKAMNIFSL 60
            MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSK       
Sbjct: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSK------- 60

Query: 61   SRSPSIRGSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSIS 120
                   GSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSIS
Sbjct: 61   -------GSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSIS 120

Query: 121  QFADSAWSDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAK 180
            QFADSAWSDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAK
Sbjct: 121  QFADSAWSDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAK 180

Query: 181  GKKGFVFNDLKGNDDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQ 240
            GKKGFVFNDLKGNDDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQ
Sbjct: 181  GKKGFVFNDLKGNDDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQ 240

Query: 241  ANSRGANKKVAHRTYFSSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDL 300
            ANSRGANKKV HRTY  SCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDL
Sbjct: 241  ANSRGANKKVPHRTYSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDL 300

Query: 301  LQNHGITICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYLYEQNGRWNDGNTG-- 360
            LQNHGITICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYL EQNGRWNDGNTG  
Sbjct: 301  LQNHGITICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGKH 360

Query: 361  ------KDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGG 420
                  KDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGG
Sbjct: 361  VNRDAPKDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGG 420

Query: 421  EEKRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANY 480
            EEKRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANY
Sbjct: 421  EEKRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANY 480

Query: 481  VEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLE 540
            VEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLE
Sbjct: 481  VEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLE 540

Query: 541  SMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNAR 600
            SMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNAR
Sbjct: 541  SMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNAR 600

Query: 601  LLITNPDMLHVSILPYHRQFSRILSNLRFVIIDEAHTYKGAFGCHTALILRRLRRICSHV 660
            LLITNPDMLHVSILPYHRQFSRILSNLRFV+IDEAHTYKGAFGCHTALILRRLRRICSHV
Sbjct: 601  LLITNPDMLHVSILPYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHV 660

Query: 661  YGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRG 720
            YGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQR 
Sbjct: 661  YGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRD 720

Query: 721  IDSLQSTEKNVNFRNPRQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTRE 780
            IDSLQSTEKNVNFRNP      PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTRE
Sbjct: 721  IDSLQSTEKNVNFRNPS-----PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTRE 780

Query: 781  ILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLH 840
            ILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLH
Sbjct: 781  ILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLH 840

Query: 841  LGFPGSIASLWQQAGRAGRREKTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGN 900
            LGFPGSIASLWQQAGRAGRRE+TSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGN
Sbjct: 841  LGFPGSIASLWQQAGRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGN 900

Query: 901  QQVLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIG 960
            QQVLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIG
Sbjct: 901  QQVLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIG 960

Query: 961  QEKMPSRAVSIRAIETERYKVIDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSL 1020
            QEKMPSRAVSIRAIETERY+V+DQRQNEVIEEIEESKAFFQ                   
Sbjct: 961  QEKMPSRAVSIRAIETERYRVVDQRQNEVIEEIEESKAFFQ------------------- 1020

Query: 1021 NLSSMLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTW 1080
                                             AYPRRFPNIPPSKTTAQANDCRVTTTW
Sbjct: 1021 ---------------------------------AYPRRFPNIPPSKTTAQANDCRVTTTW 1080

Query: 1081 FGFYRIWKGSKQIFDTVDLSLPKYSYNSQQAVWIPVPQSVKEEVKRKSFDFRAGLHAASH 1140
            FGFYRIWKGSKQIFDTVDLSLPKYSYNS QAVWIPVPQSVKEEVKRKSFDFRAGLHAASH
Sbjct: 1081 FGFYRIWKGSKQIFDTVDLSLPKYSYNS-QAVWIPVPQSVKEEVKRKSFDFRAGLHAASH 1140

Query: 1141 ALLNVVPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNA 1200
            ALLNVVPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNA
Sbjct: 1141 ALLNVVPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNA 1181

Query: 1201 ALELLTSCCCLGETGCPNCVQSLACHEYNELLHKDAASLIIKGVLDAEKSYCR 1246
            ALELLTSCCCLGETGCPNCVQSLACHEYNE+LHKDAASLIIKGVLDAEKSYCR
Sbjct: 1201 ALELLTSCCCLGETGCPNCVQSLACHEYNEVLHKDAASLIIKGVLDAEKSYCR 1181

BLAST of MS002054 vs. ExPASy TrEMBL
Match: A0A6J1C3R1 (uncharacterized protein LOC111007140 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111007140 PE=4 SV=1)

HSP 1 Score: 2197.2 bits (5692), Expect = 0.0e+00
Identity = 1110/1149 (96.61%), Postives = 1115/1149 (97.04%), Query Frame = 0

Query: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKAMNIFSL 60
            MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSK       
Sbjct: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSK------- 60

Query: 61   SRSPSIRGSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSIS 120
                   GSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSIS
Sbjct: 61   -------GSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSIS 120

Query: 121  QFADSAWSDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAK 180
            QFADSAWSDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAK
Sbjct: 121  QFADSAWSDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAK 180

Query: 181  GKKGFVFNDLKGNDDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQ 240
            GKKGFVFNDLKGNDDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQ
Sbjct: 181  GKKGFVFNDLKGNDDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQ 240

Query: 241  ANSRGANKKVAHRTYFSSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDL 300
            ANSRGANKKV HRTY  SCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDL
Sbjct: 241  ANSRGANKKVPHRTYSPSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDL 300

Query: 301  LQNHGITICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYLYEQNGRWNDGNTG-- 360
            LQNHGITICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYL EQNGRWNDGNTG  
Sbjct: 301  LQNHGITICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYLSEQNGRWNDGNTGKH 360

Query: 361  ------KDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGG 420
                  KDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGG
Sbjct: 361  VNRDAPKDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGG 420

Query: 421  EEKRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANY 480
            EEKRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANY
Sbjct: 421  EEKRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANY 480

Query: 481  VEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLE 540
            VEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLE
Sbjct: 481  VEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLE 540

Query: 541  SMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNAR 600
            SMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNAR
Sbjct: 541  SMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNAR 600

Query: 601  LLITNPDMLHVSILPYHRQFSRILSNLRFVIIDEAHTYKGAFGCHTALILRRLRRICSHV 660
            LLITNPDMLHVSILPYHRQFSRILSNLRFV+IDEAHTYKGAFGCHTALILRRLRRICSHV
Sbjct: 601  LLITNPDMLHVSILPYHRQFSRILSNLRFVLIDEAHTYKGAFGCHTALILRRLRRICSHV 660

Query: 661  YGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRG 720
            YGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQR 
Sbjct: 661  YGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRD 720

Query: 721  IDSLQSTEKNVNFRNPRQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTRE 780
            IDSLQSTEKNVNFRNP      PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTRE
Sbjct: 721  IDSLQSTEKNVNFRNPS-----PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTRE 780

Query: 781  ILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLH 840
            ILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLH
Sbjct: 781  ILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLH 840

Query: 841  LGFPGSIASLWQQAGRAGRREKTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGN 900
            LGFPGSIASLWQQAGRAGRRE+TSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGN
Sbjct: 841  LGFPGSIASLWQQAGRAGRRERTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGN 900

Query: 901  QQVLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIG 960
            QQVLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIG
Sbjct: 901  QQVLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIG 960

Query: 961  QEKMPSRAVSIRAIETERYKVIDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSL 1020
            QEKMPSRAVSIRAIETERY+V+DQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSL
Sbjct: 961  QEKMPSRAVSIRAIETERYRVVDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSL 1020

Query: 1021 NLSSMLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTW 1080
            NLSSMLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTW
Sbjct: 1021 NLSSMLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTW 1080

Query: 1081 FGFYRIWKGSKQIFDTVDLSLPKYSYNSQQAVWIPVPQSVKEEVKRKSFDFRAGLHAASH 1140
            FGFYRIWKGSKQIFDTVDLSLPKYSYNS QAVWIPVPQSVKEEVKRKSFDFRAGLHAASH
Sbjct: 1081 FGFYRIWKGSKQIFDTVDLSLPKYSYNS-QAVWIPVPQSVKEEVKRKSFDFRAGLHAASH 1129

Query: 1141 ALLNVVPLR 1142
            ALLNVVPL+
Sbjct: 1141 ALLNVVPLK 1129

BLAST of MS002054 vs. ExPASy TrEMBL
Match: A0A6J1L0Y0 (uncharacterized protein LOC111500077 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111500077 PE=4 SV=1)

HSP 1 Score: 2086.6 bits (5405), Expect = 0.0e+00
Identity = 1046/1252 (83.55%), Postives = 1122/1252 (89.62%), Query Frame = 0

Query: 1    MEESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKAMNIFSL 60
            MEESE EIRI+TLTGESLT+ ISG RTI+DLKLLLR+NFPSAT SPNFHLFSK       
Sbjct: 1    MEESESEIRIKTLTGESLTISISGDRTIEDLKLLLRKNFPSATVSPNFHLFSK------- 60

Query: 61   SRSPSIRGSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSIS 120
                   G+KLKPQS I ACRID GEFLVLIPF KKESSK QLRDQYEQ SSVS  SSIS
Sbjct: 61   -------GTKLKPQSEISACRIDKGEFLVLIPFTKKESSKPQLRDQYEQGSSVSGGSSIS 120

Query: 121  QFADSAWSDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAK 180
             FADSAWSDMVQDLSYLHDCS+QGR+ N  E ERGN E G V++EL  T ++  SS KA+
Sbjct: 121  LFADSAWSDMVQDLSYLHDCSVQGRERNGLESERGNFETGGVDSELAATCTTGPSSLKAR 180

Query: 181  GKKGFVFNDLKG-NDDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAK 240
            GKKGF+ ND  G  DD+LRNLLSS T G L+E+ CEN +KFLESVDCLSDPRN +C+LAK
Sbjct: 181  GKKGFICNDSNGILDDILRNLLSSPTVGLLSEYNCENLIKFLESVDCLSDPRNEKCLLAK 240

Query: 241  QANSRGANKKVAHRTYFSSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMD 300
            QANSR  ++K  +RT  SSCLCPVWLKKI K F+FLNV SM  QL+E+I+T SRLEQAMD
Sbjct: 241  QANSRSGSRKAPNRTRCSSCLCPVWLKKIMKTFAFLNVLSMFAQLREEIITASRLEQAMD 300

Query: 301  LLQNHGITICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYLYEQNGRWNDGNTG- 360
            LLQ HGIT+  ED+KHLSLLCPK VHFASG+LEDS DD + IV YL  QN RW   NT  
Sbjct: 301  LLQKHGITLRMEDMKHLSLLCPKVVHFASGTLEDSYDDKIIIVNYLTAQNSRWTADNTAY 360

Query: 361  -------KDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHG 420
                    D+T +KRRE+SFKFYLWEAIK HMLRHGSRSEICV FSLEDLIT K S+V G
Sbjct: 361  KLSSKAPTDITPMKRREKSFKFYLWEAIKGHMLRHGSRSEICVSFSLEDLITSKASAVDG 420

Query: 421  GEEKRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKAN 480
             E KR KKSD ASSSS+SD++QCHDTSKLLPE MVEHL+ G+GS+GQIVHVEDIAARKAN
Sbjct: 421  NEAKRAKKSDMASSSSQSDRVQCHDTSKLLPENMVEHLEKGIGSEGQIVHVEDIAARKAN 480

Query: 481  YVEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVL 540
            YVEIPEELSNNVISALKCIGVEKLYSHQ RSIEASLAG HVAVATMTSSGKSLCYNLPVL
Sbjct: 481  YVEIPEELSNNVISALKCIGVEKLYSHQTRSIEASLAGNHVAVATMTSSGKSLCYNLPVL 540

Query: 541  ESMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNA 600
            ESMSQ+V+SCALYLFPTKALAQDQLRSLLVMMKGF+ +LNIGVYDGDTSQ+DR+LLRDNA
Sbjct: 541  ESMSQDVSSCALYLFPTKALAQDQLRSLLVMMKGFNADLNIGVYDGDTSQSDRILLRDNA 600

Query: 601  RLLITNPDMLHVSILPYHRQFSRILSNLRFVIIDEAHTYKGAFGCHTALILRRLRRICSH 660
            RLLITNPDMLH+SILP HRQFSRILSNLRF++IDEAHTYKGAFGCHTALI+RRLRR+CSH
Sbjct: 601  RLLITNPDMLHLSILPQHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALIIRRLRRLCSH 660

Query: 661  VYGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQR 720
            VYGSDPSFIFCTATSANPR+HCMELG+LSSLELIENDGSPSARKLFILWNPI A K+ QR
Sbjct: 661  VYGSDPSFIFCTATSANPRQHCMELGSLSSLELIENDGSPSARKLFILWNPIMASKSSQR 720

Query: 721  GIDSLQSTEKNVNFRNPRQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTR 780
            GIDSLQSTEKN NFRNP      PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTR
Sbjct: 721  GIDSLQSTEKNANFRNPS-----PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTR 780

Query: 781  EILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATL 840
            EILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATL
Sbjct: 781  EILKERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATL 840

Query: 841  HLGFPGSIASLWQQAGRAGRREKTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAG 900
            HLGFPGSIASLWQQAGRAGRREKTSLSVYVAF+GPLDQYFMK+PEKLFGSPI+CCHIDA 
Sbjct: 841  HLGFPGSIASLWQQAGRAGRREKTSLSVYVAFEGPLDQYFMKHPEKLFGSPIKCCHIDAE 900

Query: 901  NQQVLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYI 960
            NQQVLEQHLLCAA+EHP+ ++YDQN FG GLNTAL SLK+RGDLIP PSCGSSKSIW YI
Sbjct: 901  NQQVLEQHLLCAAYEHPVCMVYDQNLFGPGLNTALMSLKSRGDLIPAPSCGSSKSIWTYI 960

Query: 961  GQEKMPSRAVSIRAIETERYKVIDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKS 1020
            G+EKMPSR+VSIRAIE ERYKV+DQRQNEV+EEIEES AFFQVYEGAVYMHQGRTYLVKS
Sbjct: 961  GKEKMPSRSVSIRAIEAERYKVVDQRQNEVLEEIEESTAFFQVYEGAVYMHQGRTYLVKS 1020

Query: 1021 LNLSSMLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTT 1080
            LNLS+MLAFCEEADLKYYTKTRDYTDIHVIGG++AYPRR PNIP  KTTAQANDCRVTTT
Sbjct: 1021 LNLSTMLAFCEEADLKYYTKTRDYTDIHVIGGSLAYPRRAPNIPLLKTTAQANDCRVTTT 1080

Query: 1081 WFGFYRIWKGSKQIFDTVDLSLPKYSYNSQQAVWIPVPQSVKEEVKRKSFDFRAGLHAAS 1140
            WFGFYRIWKGS QIFDTVDLSLPKYSYNS QAVWIPVP S+KEEVKRK++DFRAGLHAAS
Sbjct: 1081 WFGFYRIWKGSNQIFDTVDLSLPKYSYNS-QAVWIPVPLSIKEEVKRKNYDFRAGLHAAS 1140

Query: 1141 HALLNVVPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLN 1200
            HALLNVVPLRIICNMSDLAPECANPHD+RYFPERILLYDQHPGGTGMS+QIQPVFIELLN
Sbjct: 1141 HALLNVVPLRIICNMSDLAPECANPHDSRYFPERILLYDQHPGGTGMSLQIQPVFIELLN 1200

Query: 1201 AALELLTSCCCLGETGCPNCVQSLACHEYNELLHKDAASLIIKGVLDAEKSY 1244
            AALELLTSCCC GETGCPNCVQSLACHEYNE+LHKDAASLIIKGVLD EK+Y
Sbjct: 1201 AALELLTSCCCFGETGCPNCVQSLACHEYNEVLHKDAASLIIKGVLDTEKTY 1232

BLAST of MS002054 vs. ExPASy TrEMBL
Match: A0A6J1F581 (uncharacterized protein LOC111440956 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111440956 PE=4 SV=1)

HSP 1 Score: 2083.1 bits (5396), Expect = 0.0e+00
Identity = 1045/1249 (83.67%), Postives = 1123/1249 (89.91%), Query Frame = 0

Query: 3    ESEREIRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKAMNIFSLSR 62
            ESE EIRI+TLTGESLT+ ISG+RTI+DLKLLLR+NFPSAT SPNFHLFSK         
Sbjct: 5    ESESEIRIKTLTGESLTISISGNRTIEDLKLLLRKNFPSATVSPNFHLFSK--------- 64

Query: 63   SPSIRGSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQF 122
                 G+KLKPQS I ACRID GEFLVLIPF KKESSK QLRDQYEQ SSVS  SSISQF
Sbjct: 65   -----GTKLKPQSEISACRIDKGEFLVLIPFTKKESSKPQLRDQYEQGSSVSGGSSISQF 124

Query: 123  ADSAWSDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGK 182
            ADSAWSDMVQDLSYLHDCS+QGR+ N  E ERGN E G V+AEL  T S+ SSS KAKGK
Sbjct: 125  ADSAWSDMVQDLSYLHDCSVQGREGNGLESERGNFEAGGVDAELAATCSTGSSSLKAKGK 184

Query: 183  KGFVFNDLKG-NDDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQA 242
            KGFV ND  G  DD+LRNLLSS T  FL+E+ CEN +K LESVDCLSDPRN +C+LAKQA
Sbjct: 185  KGFVCNDSNGILDDILRNLLSSPTVEFLSEYNCENLIKLLESVDCLSDPRNEKCLLAKQA 244

Query: 243  NSRGANKKVAHRTYFSSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLL 302
            NSR  ++K  + T  SSCLCPVWLKKI KAF+FLNV SM  QL+E+I+T SRLEQA+DLL
Sbjct: 245  NSRSGSRKAPNGTRCSSCLCPVWLKKIMKAFAFLNVLSMFAQLREEIITASRLEQAIDLL 304

Query: 303  QNHGITICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYLYEQNGRWNDGNTG--- 362
            Q HGIT+  ED+KHLSLLCPK VHFASG+LEDS DD + IVIYL  QN RW   NT    
Sbjct: 305  QKHGITLRMEDMKHLSLLCPKVVHFASGTLEDSYDDKIIIVIYLTAQNSRWTADNTAYKL 364

Query: 363  ----KDVTLLKRRERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLITPKESSVHGGEE 422
                 D+T LKRRE+SFKFYLW+AIK HMLRHGSRSEICV FSLEDLIT K S+V G E 
Sbjct: 365  SKGPTDITPLKRREKSFKFYLWDAIKGHMLRHGSRSEICVSFSLEDLITSKASAVDGNEA 424

Query: 423  KRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVE 482
            KR KKSD ASSSSKSD++QCHDTSKLLPE MVEHL+ G+GS+GQIVHVEDIAARKANYVE
Sbjct: 425  KRAKKSDMASSSSKSDRVQCHDTSKLLPENMVEHLEKGIGSEGQIVHVEDIAARKANYVE 484

Query: 483  IPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESM 542
            IPEELSNNVISALKCIGVEKLYSHQ RSIEASLAG HVAVATMTSSGKSLCYNLPVLESM
Sbjct: 485  IPEELSNNVISALKCIGVEKLYSHQTRSIEASLAGNHVAVATMTSSGKSLCYNLPVLESM 544

Query: 543  SQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLL 602
            SQ+V+SCALYLFPTKALAQDQLRSLLVMMKGF+ +LNIGVYDGDTSQ+DR+LLRDNARLL
Sbjct: 545  SQDVSSCALYLFPTKALAQDQLRSLLVMMKGFNADLNIGVYDGDTSQSDRILLRDNARLL 604

Query: 603  ITNPDMLHVSILPYHRQFSRILSNLRFVIIDEAHTYKGAFGCHTALILRRLRRICSHVYG 662
            ITNPDMLH+SILP HRQFSRILSNLRF++IDEAHTYKGAFGCHTALI+RRLRR+CSHVYG
Sbjct: 605  ITNPDMLHLSILPQHRQFSRILSNLRFIVIDEAHTYKGAFGCHTALIIRRLRRLCSHVYG 664

Query: 663  SDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARKLFILWNPIKALKNYQRGID 722
            SDPSFIFCTATSANPR+HCMELG+LSSLELIENDGSPSARKLFILWNPI A K+ +RGID
Sbjct: 665  SDPSFIFCTATSANPRQHCMELGSLSSLELIENDGSPSARKLFILWNPIMASKSSERGID 724

Query: 723  SLQSTEKNVNFRNPRQQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREIL 782
            SLQSTEKN NFRNP      PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREIL
Sbjct: 725  SLQSTEKNANFRNPS-----PIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREIL 784

Query: 783  KERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLG 842
            KERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLG
Sbjct: 785  KERAPHLVQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLG 844

Query: 843  FPGSIASLWQQAGRAGRREKTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQ 902
            FPGSIASLWQQAGRAGRREKTSLSVYVAF+GPLDQYFMK+PEKLFGSPIECCHIDA NQQ
Sbjct: 845  FPGSIASLWQQAGRAGRREKTSLSVYVAFEGPLDQYFMKHPEKLFGSPIECCHIDAENQQ 904

Query: 903  VLEQHLLCAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQE 962
            VLE HLLCAA+EHP+ ++YDQN FG GLNTAL SLK+RGDL+P PSCGSSKSIW YIG+E
Sbjct: 905  VLEHHLLCAAYEHPVCMVYDQNLFGPGLNTALVSLKSRGDLMPAPSCGSSKSIWTYIGKE 964

Query: 963  KMPSRAVSIRAIETERYKVIDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNL 1022
            KMPSR+VSIRAIE ERYKV+DQR+NEV+EEIEES AFFQVYEGAVYMHQGRTYLVKSLNL
Sbjct: 965  KMPSRSVSIRAIEAERYKVVDQRRNEVLEEIEESTAFFQVYEGAVYMHQGRTYLVKSLNL 1024

Query: 1023 SSMLAFCEEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFG 1082
            S+MLAFCEEADLKYYTKTRDYTD+HVIGG++AYPRR PNIP  KTTAQANDCRVTTTWFG
Sbjct: 1025 STMLAFCEEADLKYYTKTRDYTDVHVIGGSLAYPRRAPNIPLLKTTAQANDCRVTTTWFG 1084

Query: 1083 FYRIWKGSKQIFDTVDLSLPKYSYNSQQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHAL 1142
            FYRIWKGS QIFDTVDLSLPKYSYNS QAVWIPVP S+KEEVKRK++DFRAGLHAASHAL
Sbjct: 1085 FYRIWKGSNQIFDTVDLSLPKYSYNS-QAVWIPVPLSIKEEVKRKNYDFRAGLHAASHAL 1144

Query: 1143 LNVVPLRIICNMSDLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNAAL 1202
            LNVVPLRIICNMSDLAPECANPHD+RYFPERILLYDQHPGGTG+S+QIQPVFIELL+AAL
Sbjct: 1145 LNVVPLRIICNMSDLAPECANPHDSRYFPERILLYDQHPGGTGVSLQIQPVFIELLHAAL 1204

Query: 1203 ELLTSCCCLGETGCPNCVQSLACHEYNELLHKDAASLIIKGVLDAEKSY 1244
            ELLTSCCC GETGCPNCVQSLACHEYNE+LHKDAASLIIKGVLDAEK+Y
Sbjct: 1205 ELLTSCCCFGETGCPNCVQSLACHEYNEVLHKDAASLIIKGVLDAEKTY 1233

BLAST of MS002054 vs. TAIR 10
Match: AT5G08110.1 (nucleic acid binding;ATP-dependent helicases;ATP binding;helicases;ATP-dependent helicases )

HSP 1 Score: 1154.0 bits (2984), Expect = 0.0e+00
Identity = 636/1241 (51.25%), Postives = 805/1241 (64.87%), Query Frame = 0

Query: 8    IRIRTLTGESLTVVISGSRTIDDLKLLLRRNFPSATNSPNFHLFSKAMNIFSLSRSPSIR 67
            I +R++ GES TV +S   TI DLK  L+  FP A++S NFHL+              I+
Sbjct: 15   ISVRSINGESTTVQVSSDGTIRDLKKALKSCFPPASSSSNFHLY--------------IK 74

Query: 68   GSKLKPQSLIGACRIDHGEFLVLIPFNKKESSKSQLRDQYEQRSSVSDRSSISQFADSAW 127
            G KLK  + + A  I+ G+ L L PF KKE  ++   D   + SS+S R+S         
Sbjct: 75   GEKLKLDTRVAAITINDGDILGLFPFKKKELRQTPKPD-LSKPSSLSSRTSTM------- 134

Query: 128  SDMVQDLSYLHDCSLQGRDENVHERERGNSEIGDVEAELVGTSSSSSSSSKAKGKKGFVF 187
                             +DEN              E   VG        +   G     F
Sbjct: 135  -----------------KDENAKR----------AEDHCVGEKRKRDEEACPYG----FF 194

Query: 188  NDLKGNDDVLRNLLSSHTEGFLNEHTCENFLKFLESVDCLSDPRNGECMLAKQANSRGAN 247
            ND         +L S   + F  ++T E   + L+S +CL+ P + +C+++  ++S    
Sbjct: 195  ND---------DLESECKDAFKGQNT-EKLAEVLKSRNCLTSPGSTKCLMSWDSSS---- 254

Query: 248  KKVAHRTYFSSCLCPVWLKKIRKAFSFLNVFSMILQLQEKIMTVSRLEQAMDLLQNHGIT 307
                     S C CP W    R A S                               G+ 
Sbjct: 255  ---------SLCSCPDWESLSRLAMS-------------------------------GVR 314

Query: 308  ICTEDIKHLSLLCPKAVHFASGSLEDSCDDTLSIVIYLYEQNGRWNDGNTGKDVTLLKRR 367
            +  +D+K+LS++CPK +     ++       ++  + + E++GR       K  + +K+R
Sbjct: 315  VRIQDVKNLSVICPKVITDDYEAVNYENAIVIADYLEMDEKSGR-KKIPLAKLFSAMKKR 374

Query: 368  ERSFKFYLWEAIKFHMLRHGSRSEICVPFSLEDLIT-PKESSVHGGEEKRRK-KSDTASS 427
            E SFK   WE+I+  + ++   S I +  SLE L+    E    GG E R+  K    +S
Sbjct: 375  EASFKSDFWESIRSLLNKNTGESGIAI--SLEGLLKFASEGRADGGNEARQAGKGTCPTS 434

Query: 428  SSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQIVHVEDIAARKANYVEIPEELSNNVIS 487
             S+  Q  CH T+ LLP  MVEHL+ G+GS GQ+VHVE I ARK+ YVE+ ++LS    S
Sbjct: 435  GSRKFQTLCHATNSLLPSEMVEHLRNGIGSKGQVVHVEVIKARKSAYVEMSDKLSETTKS 494

Query: 488  ALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSGKSLCYNLPVLESMSQNVTSCALYL 547
            ALK IG+  LYSHQA +I A+LAGK+VAVATMTSSGKSLCYN+PV E + ++  SCALYL
Sbjct: 495  ALKRIGLNTLYSHQAEAISAALAGKNVAVATMTSSGKSLCYNVPVFEELCKDTNSCALYL 554

Query: 548  FPTKALAQDQLRSLLVMMKGFSDNLNIGVYDGDTSQADRVLLRDNARLLITNPDMLHVSI 607
            FPTKALAQDQLR+L  ++KGF  ++N+GVYDGDT   DR  LR NARLLITNPDMLH+SI
Sbjct: 555  FPTKALAQDQLRALSDLIKGFEASINMGVYDGDTPYKDRTRLRSNARLLITNPDMLHISI 614

Query: 608  LPYHR-QFSRILSNLRFVIIDEAHTYKGAFGCHTALILRRLRRICSHVYGSDPSFIFCTA 667
            L  H+ QFSRILSNLR+++IDEAH YKG FGCH ALILRRLRR+CSHVYG +PSFIFCTA
Sbjct: 615  LRRHKEQFSRILSNLRYIVIDEAHIYKGPFGCHMALILRRLRRLCSHVYGVNPSFIFCTA 674

Query: 668  TSANPREHCMELGNLSSLELIENDGSPSARKLFILWNP-IKALKNYQRGIDSLQSTEKNV 727
            TSANPREHCMEL NLS LEL+  DGSPS+ KLF+LWNP        ++    + S+E   
Sbjct: 675  TSANPREHCMELANLSELELVTIDGSPSSEKLFVLWNPSAPPTSKSEKSSKVVSSSEAAA 734

Query: 728  NFRNPR--QQLSIPIMDIARLFAEMVQHGLRCIAFCKTRKLCELVLCYTREILKERAPHL 787
            +  +      LS P  +++ LFAEMVQHGLRCIAFC +RKLCELVLC TREIL E APHL
Sbjct: 735  DKPSGAAVDTLSGPASEVSHLFAEMVQHGLRCIAFCPSRKLCELVLCLTREILAETAPHL 794

Query: 788  VQSVCAYRAGYTAEDRRRIESDFFGGNLCGVAATNALELGIDVGHIDATLHLGFPGSIAS 847
            V+++ +YR GY AEDRR+IESD FGG LCG+AATNALELGIDVGHID TLHLGFPGSIAS
Sbjct: 795  VEAITSYRGGYIAEDRRKIESDLFGGKLCGIAATNALELGIDVGHIDVTLHLGFPGSIAS 854

Query: 848  LWQQAGRAGRREKTSLSVYVAFDGPLDQYFMKNPEKLFGSPIECCHIDAGNQQVLEQHLL 907
            LWQQAGR+GRR+K SL+VYVAF GPLDQY+M  P+KLFGSPIECCHID+ N+ VL QHL 
Sbjct: 855  LWQQAGRSGRRQKPSLAVYVAFLGPLDQYYMSFPDKLFGSPIECCHIDSQNKHVLMQHLA 914

Query: 908  CAAHEHPISVLYDQNFFGSGLNTALTSLKNRGDLIPDPSCGSSKSIWNYIGQEKMPSRAV 967
            CAA EHP+S+ YDQ  FGSGL+  L  L+N+G L  DPS  SS  IWNYIG+EK P+R V
Sbjct: 915  CAALEHPLSLQYDQQHFGSGLSDPLAELRNKGFLSFDPSRDSSSRIWNYIGREKNPARIV 974

Query: 968  SIRAIETERYKVIDQRQNEVIEEIEESKAFFQVYEGAVYMHQGRTYLVKSLNLSSMLAFC 1027
            SIRAIET RY+V++++  +V++EIEESKAFF VYEGA+YM+QGR YLV SL++   +A C
Sbjct: 975  SIRAIETVRYRVMEKKSKDVLDEIEESKAFFHVYEGAIYMNQGRNYLVTSLDIKEKVALC 1034

Query: 1028 EEADLKYYTKTRDYTDIHVIGGNMAYPRRFPNIPPSKTTAQANDCRVTTTWFGFYRIWKG 1087
            E  ++ YYT+TRDYTDI V GG+ AYP +     P K T Q + CRVTT WFGF RI + 
Sbjct: 1035 ELVNVDYYTRTRDYTDIKVTGGDTAYPVK----APKKPTPQTHACRVTTKWFGFLRIRRR 1094

Query: 1088 SKQIFDTVDLSLPKYSYNSQQAVWIPVPQSVKEEVKRKSFDFRAGLHAASHALLNVVPLR 1147
            + ++ D V+LSLP Y+Y S QAVWI VP SVK  V+  +  FRAGLHAA HAL+NVVP R
Sbjct: 1095 NNEVIDDVELSLPSYTYQS-QAVWIQVPMSVKLAVETANLPFRAGLHAACHALVNVVPTR 1140

Query: 1148 IICNMSDLAPECANPHDTRYFPERILLYDQHPGGTGMSVQIQPVFIELLNAALELLTSC- 1207
            + CN SD+APEC NP + RYFP RIL+YD+HPGGTG+S +I P+F ELL  A +LL SC 
Sbjct: 1155 VTCNYSDIAPECPNPQEQRYFPARILVYDRHPGGTGISAKICPLFFELLKDARDLLRSCE 1140

Query: 1208 CCLGETGCPNCVQSLACHEYNELLHKDAASLIIKGVLDAEK 1242
             C  ETGCP C Q+  C  YNELLHK AA +I++GVLDA++
Sbjct: 1215 KCPPETGCPKCAQTFGCRGYNELLHKKAAIMIMQGVLDAKE 1140

BLAST of MS002054 vs. TAIR 10
Match: AT1G27880.1 (DEAD/DEAH box RNA helicase family protein )

HSP 1 Score: 69.7 bits (169), Expect = 1.9e-11
Identity = 95/408 (23.28%), Postives = 169/408 (41.42%), Query Frame = 0

Query: 455 SDGQIVHVED-IAARKANYVEIPEELSNNVISALKCI-GVEKLYSHQARSIEASLAGKHV 514
           ++G I  VED I A K       E    N+   L  + G +     Q ++I+  L G   
Sbjct: 234 ANGFISSVEDAILAVKT------EASDENLTKLLNLVYGYDSFRDGQLQAIKMILGGSST 293

Query: 515 AVATMTSSGKSLCYNLPVLESMSQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNI 574
            +   T +GKSLCY +P +      +    L + P  +L  DQL+ L  ++KG    L+ 
Sbjct: 294 MLVLPTGAGKSLCYQIPAM-----ILPGITLVVSPLVSLMIDQLKHLPSIIKG--GLLSS 353

Query: 575 GVYDGDTSQADRVLLRDNARLLITNPD-MLHVSILPYHRQFSRILSNLRFVIIDEAH-TY 634
                + ++  R L     ++L  +P+ +L+V  L       R+  ++  V++DEAH   
Sbjct: 354 SQRPEEATETLRKLKEGIIKVLFVSPERLLNVEFL----SMFRMSLSVSLVVVDEAHCVS 413

Query: 635 KGAFGCHTALILRRLRRICSHVYGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGS 694
           + +     + +  +   + S +       +  TAT+   +        +SSLE+      
Sbjct: 414 EWSHNFRPSYMRLKASMLFSELKAECILAMTATATTMTLQ------AVMSSLEI------ 473

Query: 695 PSARKLFILWNPIKALKNYQRGIDSLQSTEKNVNFRNPRQQLSIPIMDIARLFAEMVQHG 754
           PS               N  +      + E +V+     +   + I+  +  + E+    
Sbjct: 474 PST--------------NLIQKSQLRDNFELSVSLSGANRMKDLLILMESPPYKEI---- 533

Query: 755 LRCIAFCKTRKLCELVLCYTREILKERAPHLVQSVCA--YRAGYTAEDRRRIESDFFGGN 814
              I +CK +   +++  Y R+           ++ A  Y +G  A+DR RI+  F    
Sbjct: 534 RSIIVYCKFQYETDMISKYLRD----------NNINAKGYHSGLPAKDRVRIQESFCSNK 584

Query: 815 LCGVAATNALELGIDVGHIDATLHLGFPGSIASLWQQAGRAGRREKTS 857
           +  V AT A  +G+D G + A +H   PGS+    Q+ GRAGR  + S
Sbjct: 594 IRVVVATVAFGMGLDKGDVGAVIHFSVPGSMEEYVQEIGRAGRDGRLS 584

BLAST of MS002054 vs. TAIR 10
Match: AT4G16630.1 (DEA(D/H)-box RNA helicase family protein )

HSP 1 Score: 51.6 bits (122), Expect = 5.3e-06
Identity = 61/239 (25.52%), Postives = 104/239 (43.51%), Query Frame = 0

Query: 462 VEDIAARKANYVEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAVATMTSSG 521
           V+ ++     ++E+   LS  ++ A + +G +K    QA  I  +L G+ +  + +T SG
Sbjct: 159 VDGVSFHADTFMEL--NLSRPLLRACETLGYKKPTPIQAACIPLALTGRDLCASAITGSG 218

Query: 522 KSLCYNLPVLESM----SQNVTSCALYLFPTKALAQDQLRSLLVMMKGFSDNLNIGVYDG 581
           K+  + LP LE +     +   +  L L PT+ LA  Q+ S++  +  F+D +  G+  G
Sbjct: 219 KTAAFALPTLERLLFRPKRVFATRVLILTPTRELAV-QIHSMIQNLAQFTD-IKCGLIVG 278

Query: 582 DTS-QADRVLLRDNARLLITNPDMLHVSILPYHRQFSRILSNLRFVIIDEAHTYKGAFGC 641
             S +   V+LR    +++  P  +   I       S  L +L  +I+DEA         
Sbjct: 279 GLSVREQEVVLRSMPDIVVATPGRM---IDHLRNSMSVDLDDLAVLILDEADR---LLQT 338

Query: 642 HTALILRRLRRICSHVYGSDPSFIFCTATSANPREHCMELGNLSSLELIENDGSPSARK 696
             A  +  L R+C       P        SA   E   EL  LS  + +     PSAR+
Sbjct: 339 GFATEITELVRLC-------PKRRQTMLFSATMTEEVKELVKLSLNKPLRLSADPSARR 380

BLAST of MS002054 vs. TAIR 10
Match: AT5G62190.1 (DEAD box RNA helicase (PRH75) )

HSP 1 Score: 46.6 bits (109), Expect = 1.7e-04
Identity = 59/247 (23.89%), Postives = 103/247 (41.70%), Query Frame = 0

Query: 401 LITPKESSVHGGEEKRRKKSDTASSSSKSDQIQCHDTSKLLPEIMVEHLKAGVGSDGQ-- 460
           L TP+  S  G +E++ K SD+    S+  + +  D  +   E   + +K+   S+ +  
Sbjct: 21  LDTPELDSKKGKKEQKLKLSDSDEEESEKKKSKKKDKKRKASE-EEDEVKSDSSSEKKKS 80

Query: 461 ----IVHVEDIAARKANYVEIPEELSNNVISALKCIGVEKLYSHQARSIEASLAGKHVAV 520
                + VED+     N V     +S  +   LK  G+E L+  QA + +  L G  +  
Sbjct: 81  SKKVKLGVEDVEVDNPNAVS-KFRISAPLREKLKANGIEALFPIQASTFDMVLDGADLVG 140

Query: 521 ATMTSSGKSLCYNLPVLES-----------MSQNVTSCALYLFPTKALAQDQLRSLLVMM 580
              T  GK+L + LP+LES           M    +   L L PT+ LA    + +    
Sbjct: 141 RARTGQGKTLAFVLPILESLVNGPAKSKRKMGYGRSPSVLVLLPTRELA----KQVAADF 200

Query: 581 KGFSDNLNIG---VYDGDTSQADRVLLRDNARLLITNPDMLHVSILPYHRQFSRILSNLR 628
             +  +L +    +Y GD+       L+    +++  P  +   I   +  F    S L+
Sbjct: 201 DAYGGSLGLSSCCLYGGDSYPVQEGKLKRGVDIVVGTPGRIKDHIERQNLDF----SYLQ 257