MS001996 (gene) Bitter gourd (TR) v1

Overview


Name	MS001996
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	protein EMBRYO DEFECTIVE 514
Location	scaffold30: 1552627 .. 1555354 (+)
RNA-Seq Expression	MS001996
Synteny	MS001996

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGAAGAAACAACTCCTCAACTCGCTGAAAACGATCCGGAGGTAACCAACTCGGCCACCGAAGATATGGAACTGGAAACCTCAGAGCCCGGGGCAGAACCGAAAGGATTAAATGGTGATTCTGAAGCAAATGAGGCCGCTCCAGAAACGAATGGGGATGCAAATTCGAAGCGTGAGAGAGAGGAAGAAACCGCCGATGAGAATATCGGCGAGGCGAAAAAGCCGAAGGTGGAGAAGTCGGTCGAAGAGGAAAGGATGGAGAAGGTGGAAGGAGACGGGAAGGGAGATGAGAAGTCTGGTCCTGTGAGCCTAGGCCCGAAGAGCTTTGGATCATCGGTGGAGATGTTCGATTACTTCTACAAGTTCCTCCATTACTGGCCTGCCAACTTGAACTTGAACGAGGTATAGTATTATAGACTTCTGTCATCTGCTACAGTCCTGTTTTGTTAATGGTAATGTAGAAGAATTTTGAATTTATTTTGCTTTGAAGGGAGATTTGGAACTTGTTTGAACGAATTTTCTACGGTATCCCGTTGGTTTTGTTCATCATTATTATTTTGTGTGTTTAAAAGATACATATCGCTTAAGAGGATTAAATTACGAAAGTAGGTGGGGAATTAGGCGTCTCAAACCTCATGAAAGGTGGACGGTTCGTTAGTTTTAGGTTTAGCAGAAGGAGGCTCTTGTTCAATTTGATATAATGCTTTTATGTCTTATGAATTATTCTGTTGTCGGTAAAGTAGCTTGGGTTTATAATCTATTGTGGACGCAAGTCAATTCATACTTAATTATCGTAGTTGTATTGTTGAAGAAAAAGCTTGTTTTTGTGCACATGTTGGAATCTGAGGCGTGTCATTCTTCAATATTCTGATGGAGATTGATGAGGGTTTCTTCTTTACTTTTAAGAGTTTGAGGTCTTTACTTCTCAGAGTTCCTTAATAGGCACACTAAATTAAATGTGTATTATATTTTTGTCGATCAACAATAATACTCCTTGCAAGCAAGCAACTGGTTGAATATTTGAAATTATGATCAGCATTAGTTTTTCATGAAAGGTTTATTTAAGTGTCCTGTAATGTATTTGGTGAAATATTATTTGTCTGCCTGTGCATGTTTGTTTATAAGGAGATCTCTAAATTTATTTGTTATTTGAATCAAATGCTGGCTTGTCGAATTGTTTAAGTCTTTCTGTGAACGATTGAGGATATTTTAATTGGAAATCATAAAGATCTTCTCGGCTCTTCTATCAGTTTGATTATTAATTCGTGTTGGTGATTCAATTTTATACAATTTCCATTTAAAAAGAAAAAGGAAAAAAAAATTCTGGTTAATTTGGTTATGATTGTTTTGCTGATGTTTTTAGTCAATTCTGGTTGATTTGGCTGAATAGTNAAAAAAAAAAGAAAAGGTTTTTCTGAATCAGTTTTCAAAATTTGCCTAAACCGTCTGTTGGTTTAGTGCAAGTTTTTGTCAAAACCCAATTACATCTTTAGATGGTACTGTTTGACATAATTTGTCATAGGTAATGGAATATATGTGGTAAGTAGAAAATCTCCACCCCTGGAAGAATTAATTTTATTTGGTTCTTTGATTGAATACTCTATTGGCCTGATTTGCTCATTGAATAATTTCTTTCAGAAGCTTATTCGGCATTGTTATGTTGTTATTCTTCATGATACCCTTATTTCTTATTCTTGATTGATTTTAGTGGAAGAAATAGACGTGCTTTATAATCCAACAAAATCATTCATTTCTTGGGGGTGGGCAGGGAGGGTCAAAGCTTATTATATGTGTAGTACTTTCTTGCTCATTCTGTGATGTAGCAAAGTATACGAACAAATATAGCATGGCTCTTCTGAAACAAAAGCAAGATCCACACAGAGCCAGTGAGCCACTGTGGCATAAAATGTTGGGGCAAGTTATGATTATTGTTTTGAAATCTATGTGTTCATGGCCGGTTAAATCAAAAGGCTCAAGTGCTTATTTGTATTGGCAGCAATCATTAGTACTTCTGTTGTATTTTATTTCAATATTTTTTTCACTTTCTGTGAAAATCTTGGTTCACATAGTATTCAACCTTACCATAACTAATTAATTATGACATCAACAACTTCCTTCATTAGTTCAAATTTCTGTCATATGTTTGAATTGTAATGTCATGAATTCAAATTTCTGTCATAAATTCCTTAATGCACCTAGTATTATTTTTTCAGTCGTGTTTTCCATTTATCAGTAGCCGTTCAAATGGCAAAAATTTCCACCATTTTTAGCCCTTGATGGTCTTCAACTTGATACGGAACATTAATAATTTGATCAAATTCAATGTTGAAGAAATTGATTCATGGTAACAATGTTTGCATGTTGAGGAGCATTAATCGTATAGATGATCCAATATTTGATTTTTTGTTTGTGGCAGTACGAGCAGATGGTGCTGCTGGAGTTGCTCAAGAAGGGCCATGTAGAACCAGAGAAAAAGATCGGTTGTGGTATCCACGCGTTCCAGATTCGAAACCATCCTACATTTAAAAGCAAGTGCTACTTCCTGATTCGGGAAGATGAGTCCGTGGATGACTTCAGCTTCAGGAAGTGTGTGGATCACATCCTTCCCCTCCCTGAAAACTTGAAGGTAAAATCTGATGCAAACAGAGCATTAGGAGGCGGTAAGCATCGTGGCGGGGGACGGGGAGGCGGTGGTGGGCGTGGACGAGGGGGTAGGTCGAGAAAC

mRNA sequence

ATGGCAGAAGAAACAACTCCTCAACTCGCTGAAAACGATCCGGAGGTAACCAACTCGGCCACCGAAGATATGGAACTGGAAACCTCAGAGCCCGGGGCAGAACCGAAAGGATTAAATGGTGATTCTGAAGCAAATGAGGCCGCTCCAGAAACGAATGGGGATGCAAATTCGAAGCGTGAGAGAGAGGAAGAAACCGCCGATGAGAATATCGGCGAGGCGAAAAAGCCGAAGGTGGAGAAGTCGGTCGAAGAGGAAAGGATGGAGAAGGTGGAAGGAGACGGGAAGGGAGATGAGAAGTCTGGTCCTGTGAGCCTAGGCCCGAAGAGCTTTGGATCATCGGTGGAGATGTTCGATTACTTCTACAAGTTCCTCCATTACTGGCCTGCCAACTTGAACTTGAACGAGTACGAGCAGATGGTGCTGCTGGAGTTGCTCAAGAAGGGCCATGTAGAACCAGAGAAAAAGATCGGTTGTGGTATCCACGCGTTCCAGATTCGAAACCATCCTACATTTAAAAGCAAGTGCTACTTCCTGATTCGGGAAGATGAGTCCGTGGATGACTTCAGCTTCAGGAAGTGTGTGGATCACATCCTTCCCCTCCCTGAAAACTTGAAGGTAAAATCTGATGCAAACAGAGCATTAGGAGGCGGTAAGCATCGTGGCGGGGGACGGGGAGGCGGTGGTGGGCGTGGACGAGGGGGTAGGTCGAGAAAC

Coding sequence (CDS)

ATGGCAGAAGAAACAACTCCTCAACTCGCTGAAAACGATCCGGAGGTAACCAACTCGGCCACCGAAGATATGGAACTGGAAACCTCAGAGCCCGGGGCAGAACCGAAAGGATTAAATGGTGATTCTGAAGCAAATGAGGCCGCTCCAGAAACGAATGGGGATGCAAATTCGAAGCGTGAGAGAGAGGAAGAAACCGCCGATGAGAATATCGGCGAGGCGAAAAAGCCGAAGGTGGAGAAGTCGGTCGAAGAGGAAAGGATGGAGAAGGTGGAAGGAGACGGGAAGGGAGATGAGAAGTCTGGTCCTGTGAGCCTAGGCCCGAAGAGCTTTGGATCATCGGTGGAGATGTTCGATTACTTCTACAAGTTCCTCCATTACTGGCCTGCCAACTTGAACTTGAACGAGTACGAGCAGATGGTGCTGCTGGAGTTGCTCAAGAAGGGCCATGTAGAACCAGAGAAAAAGATCGGTTGTGGTATCCACGCGTTCCAGATTCGAAACCATCCTACATTTAAAAGCAAGTGCTACTTCCTGATTCGGGAAGATGAGTCCGTGGATGACTTCAGCTTCAGGAAGTGTGTGGATCACATCCTTCCCCTCCCTGAAAACTTGAAGGTAAAATCTGATGCAAACAGAGCATTAGGAGGCGGTAAGCATCGTGGCGGGGGACGGGGAGGCGGTGGTGGGCGTGGACGAGGGGGTAGGTCGAGAAAC

Protein sequence

MAEETTPQLAENDPEVTNSATEDMELETSEPGAEPKGLNGDSEANEAAPETNGDANSKREREEETADENIGEAKKPKVEKSVEEERMEKVEGDGKGDEKSGPVSLGPKSFGSSVEMFDYFYKFLHYWPANLNLNEYEQMVLLELLKKGHVEPEKKIGCGIHAFQIRNHPTFKSKCYFLIREDESVDDFSFRKCVDHILPLPENLKVKSDANRALGGGKHRGGGRGGGGGRGRGGRSRN

Homology

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BLAST of MS001996 vs. NCBI nr
Match: XP_022135230.1 (protein EMBRYO DEFECTIVE 514 [Momordica charantia])

HSP 1 Score: 449.5 bits (1155), Expect = 1.8e-122
Identity = 237/238 (99.58%), Postives = 237/238 (99.58%), Query Frame = 0


Query: 1   MAEETTPQLAENDPEVTNSATEDMELETSEPGAEPKGLNGDSEANEAAPETNGDANSKRE 60
           MAEETTPQLAENDPEVTNSATEDMELETSEPGAEPKGLNGDSEANEAAPETNGDANSKRE
Sbjct: 1   MAEETTPQLAENDPEVTNSATEDMELETSEPGAEPKGLNGDSEANEAAPETNGDANSKRE 60

Query: 61  REEETADENIGEAKKPKVEKSVEEERMEKVEGDGKGDEKSGPVSLGPKSFGSSVEMFDYF 120
           REEETADENIGEAKKPKVEKSVEEERMEKVEGDGKGDEKSGPVSLGPKSFGSSVEMFDYF
Sbjct: 61  REEETADENIGEAKKPKVEKSVEEERMEKVEGDGKGDEKSGPVSLGPKSFGSSVEMFDYF 120

Query: 121 YKFLHYWPANLNLNEYEQMVLLELLKKGHVEPEKKIGCGIHAFQIRNHPTFKSKCYFLIR 180
           YKFLHYWPANLNLNEYEQMVLLELLKKGHVEPEKKIGCGIHAFQIRNHPTFKSKCYFLIR
Sbjct: 121 YKFLHYWPANLNLNEYEQMVLLELLKKGHVEPEKKIGCGIHAFQIRNHPTFKSKCYFLIR 180

Query: 181 EDESVDDFSFRKCVDHILPLPENLKVKSDANRALGGGKHRGGGRGGGGGRGRGGRSRN 239
           EDESVDDFSFRKCVDHILPLPENLKVKSDANRALGG KHRGGGRGGGGGRGRGGRSRN
Sbjct: 181 EDESVDDFSFRKCVDHILPLPENLKVKSDANRALGGCKHRGGGRGGGGGRGRGGRSRN 238

[Close]

BLAST of MS001996 vs. NCBI nr
Match: XP_022933555.1 (protein EMBRYO DEFECTIVE 514-like [Cucurbita moschata])

HSP 1 Score: 352.4 bits (903), Expect = 3.0e-93
Identity = 186/228 (81.58%), Postives = 203/228 (89.04%), Query Frame = 0


Query: 1   MAEETTPQLAENDPEVTNSATEDMELETSEPGAEPKGLNGDSEANEAAPETNGDANSKRE 60
           MAEET PQLAEN PEVTNSA EDMELE++    EPK   GDS+ANEAAP TNGDANSKRE
Sbjct: 1   MAEETIPQLAENGPEVTNSAAEDMELEST----EPKVSGGDSDANEAAPVTNGDANSKRE 60

Query: 61  REEETADENIGEAKKPKVEKSVEEERMEKVEGDGKGDEKSGPVSLGPKSFGSSVEMFDYF 120
           REEE AD++ GEAKKPKVEKSVEEER+EKVE DGKG+E+ GPVSLGPKSFGSSVE+FDYF
Sbjct: 61  REEEGADDHAGEAKKPKVEKSVEEERLEKVERDGKGEEEHGPVSLGPKSFGSSVELFDYF 120

Query: 121 YKFLHYWPANLNLNEYEQMVLLELLKKGHVEPEKKIGCGIHAFQIRNHPTFKSKCYFLIR 180
           Y FLHYWPANLN+N+YE MVLL+L+KKGHVEP+KKIGCG+ A QIR HP +KSKC+FLIR
Sbjct: 121 YNFLHYWPANLNVNQYELMVLLDLIKKGHVEPDKKIGCGVRACQIRYHPMWKSKCFFLIR 180

Query: 181 EDESVDDFSFRKCVDHILPLPENLKVKSDANRALGGGKHRGGGRGGGG 229
           ED S DDFSFRKCVDHILPLPENLK  SDANRALGGGKHRGGGRGGGG
Sbjct: 181 EDGSADDFSFRKCVDHILPLPENLKTISDANRALGGGKHRGGGRGGGG 224

[Close]

BLAST of MS001996 vs. NCBI nr
Match: KAG7021514.1 (Protein EMBRYO DEFECTIVE [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 349.7 bits (896), Expect = 1.9e-92
Identity = 187/230 (81.30%), Postives = 203/230 (88.26%), Query Frame = 0


Query: 1   MAEETTPQLAENDPEVTNSATEDMELETSEPGAEPKGLNGDSEANEAAPETNGDANSKRE 60
           MAEET PQLAEN PEVTNSA EDMELE++    EPK   GDSEANEAAP TNGDANSKRE
Sbjct: 1   MAEETIPQLAENGPEVTNSAAEDMELEST----EPKVSGGDSEANEAAPVTNGDANSKRE 60

Query: 61  REEETADENIGEAKKPKVEKSVEEERMEKVEGDGKGDEKSGPVSLGPKSFGSSVEMFDYF 120
           REEE AD++ GEAKKPKVEKSVEEER+EKVE DGKG+E+ GPVSLGPKSFGSSVE+FDYF
Sbjct: 61  REEEGADDHAGEAKKPKVEKSVEEERLEKVERDGKGEEEHGPVSLGPKSFGSSVELFDYF 120

Query: 121 YKFLHYWPANLNLNEYEQMVLLELLKKGHVEPEKKIGCGIHAFQIRNHPTFKSKCYFLIR 180
           Y FLHYWPANLN+N+YE MVLL+L+KKGHVEP+KKIGCG+ A QIR HP +KSKC+FLIR
Sbjct: 121 YNFLHYWPANLNVNQYELMVLLDLIKKGHVEPDKKIGCGVRACQIRYHPMWKSKCFFLIR 180

Query: 181 EDESVDDFSFRKCVDHILPLPENLKVKSDANRALGGGKHRGGGRGGGGGR 231
           ED S DDFSFRKCVDHILPLPENLK  SDANRALGGGKHRGGGRGGG  R
Sbjct: 181 EDGSADDFSFRKCVDHILPLPENLKTISDANRALGGGKHRGGGRGGGRWR 226

[Close]

BLAST of MS001996 vs. NCBI nr
Match: XP_022972943.1 (protein EMBRYO DEFECTIVE 514-like [Cucurbita maxima])

HSP 1 Score: 348.6 bits (893), Expect = 4.3e-92
Identity = 184/228 (80.70%), Postives = 202/228 (88.60%), Query Frame = 0


Query: 1   MAEETTPQLAENDPEVTNSATEDMELETSEPGAEPKGLNGDSEANEAAPETNGDANSKRE 60
           MAEET PQLAEN PEVTNSA EDMELE++    EPK   GDS+ANEAAP TNGDANSKRE
Sbjct: 1   MAEETIPQLAENGPEVTNSAAEDMELEST----EPKVSGGDSDANEAAPVTNGDANSKRE 60

Query: 61  REEETADENIGEAKKPKVEKSVEEERMEKVEGDGKGDEKSGPVSLGPKSFGSSVEMFDYF 120
           REEE A + +GEAKKPKVEKSVEEER+EKVE DGKG+E+ GPVSLGPKSFGSSVE+F+YF
Sbjct: 61  REEEGAGDYVGEAKKPKVEKSVEEERLEKVERDGKGEEEHGPVSLGPKSFGSSVELFEYF 120

Query: 121 YKFLHYWPANLNLNEYEQMVLLELLKKGHVEPEKKIGCGIHAFQIRNHPTFKSKCYFLIR 180
           Y FLHYWPANLN+N+YE MVLL+L+KKGHVEP+KKIGCG+ A QIR HP +KSKC+FLIR
Sbjct: 121 YNFLHYWPANLNVNQYELMVLLDLIKKGHVEPDKKIGCGVRACQIRYHPMWKSKCFFLIR 180

Query: 181 EDESVDDFSFRKCVDHILPLPENLKVKSDANRALGGGKHRGGGRGGGG 229
           ED S DDFSFRKCVDHILPLPENLK  SDANRALGGGKHRGGGRGGGG
Sbjct: 181 EDGSADDFSFRKCVDHILPLPENLKTISDANRALGGGKHRGGGRGGGG 224

[Close]

BLAST of MS001996 vs. NCBI nr
Match: XP_023529405.1 (protein EMBRYO DEFECTIVE 514-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 343.2 bits (879), Expect = 1.8e-90
Identity = 182/228 (79.82%), Postives = 200/228 (87.72%), Query Frame = 0


Query: 1   MAEETTPQLAENDPEVTNSATEDMELETSEPGAEPKGLNGDSEANEAAPETNGDANSKRE 60
           MAEET PQL EN PEVTNS+ EDMELE++    EPK   GDS+ANEAAP TNGDANSKRE
Sbjct: 1   MAEETIPQLGENGPEVTNSSAEDMELEST----EPKVSGGDSDANEAAPVTNGDANSKRE 60

Query: 61  REEETADENIGEAKKPKVEKSVEEERMEKVEGDGKGDEKSGPVSLGPKSFGSSVEMFDYF 120
           REEE A + +GEAKK KVEKSVEEER+EKVE DGKG+E+ GPVSLGPKSFGSSVE+FDYF
Sbjct: 61  REEEGAGDCVGEAKKLKVEKSVEEERLEKVERDGKGEEEHGPVSLGPKSFGSSVELFDYF 120

Query: 121 YKFLHYWPANLNLNEYEQMVLLELLKKGHVEPEKKIGCGIHAFQIRNHPTFKSKCYFLIR 180
           Y FLHYWPANLN+N+YE MVLL+L+KKGHVEP+KKIGCG+ A QIR HP +KSKC+FLIR
Sbjct: 121 YNFLHYWPANLNVNQYELMVLLDLIKKGHVEPDKKIGCGVRACQIRYHPMWKSKCFFLIR 180

Query: 181 EDESVDDFSFRKCVDHILPLPENLKVKSDANRALGGGKHRGGGRGGGG 229
           ED S DDFSFRKCVDHILPLPENLK  SDANRALGGGKHRGGGRGGGG
Sbjct: 181 EDGSADDFSFRKCVDHILPLPENLKTISDANRALGGGKHRGGGRGGGG 224

[Close]

BLAST of MS001996 vs. ExPASy Swiss-Prot
Match: Q8L557 (Protein EMBRYO DEFECTIVE 514 OS=Arabidopsis thaliana OX=3702 GN=EMB514 PE=1 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 3.6e-49
Identity = 119/223 (53.36%), Postives = 142/223 (63.68%), Query Frame = 0


Query: 15  EVTNSATEDMELETSEPGAEPKGLNGDSEANEAAPETNGDANSKREREEETADENIGEAK 74
           EV      DME+ET+ P AE                  GD   KREREE   +EN GE+K
Sbjct: 14  EVNPDQKVDMEVETATPKAE-----------------TGD--EKREREETEEEENGGESK 73

Query: 75  KPKVEKSVEEERMEKVEGDGKGDEKSGPVSLGPKSFGSSVEMFDYFYKFLHYWPANLNLN 134
           K KV +                +EKSGPV LGPK F +SV MFDYF KFLH+WP +L++N
Sbjct: 74  KQKVGE----------------EEKSGPVKLGPKEFVTSVAMFDYFVKFLHFWPTDLDVN 133

Query: 135 EYEQMVLLELLKKGHVEPEKKIGCGIHAFQIRNHPTFKSKCYFLIREDESVDDFSFRKCV 194
           +YE MVLL+L+KKGH EPEKKIG GI  FQ+R HP +KS+C+FL+RED++ DDFSFRKCV
Sbjct: 134 KYEHMVLLDLIKKGHSEPEKKIGGGIKTFQVRTHPMWKSRCFFLVREDDTADDFSFRKCV 193

Query: 195 DHILPLPENLKVKSDANRALGGGKHRGGGRGGGGGRGRGGRSR 238
           D ILPLPEN+K         GGG+  GGGR GG G GRGGR R
Sbjct: 194 DQILPLPENMKTPGANGNGHGGGRGGGGGRRGGRGGGRGGRFR 201

[Close]

BLAST of MS001996 vs. ExPASy Swiss-Prot
Match: Q9C642 (Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 2.2e-06
Identity = 50/163 (30.67%), Postives = 80/163 (49.08%), Query Frame = 0


Query: 53  GDANSKREREEETADENIGE------------AKKPKV--EKSVEEERMEKVEGDGKGDE 112
           GD+ S R R      E+ G              ++P++  E+S EEE  E+ E + +GDE
Sbjct: 38  GDSTSLRPRVRALRTESDGAKIGNSESYGSELLRRPRIASEESSEEEE-EEEEENSEGDE 97

Query: 113 KSGPVSLGPKSFGSSVEMFDYFYKFLH--YWPANLNLNEYEQMVLLELLKKGHVEPEKKI 172
               V    K    +V +  +    LH   +     L+   +  ++E+L   H E EKKI
Sbjct: 98  F---VDWEDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKKI 157

Query: 173 GCGIHAFQIRNHPTFK-SKCYFLIREDESVDDFSFRKCVDHIL 199
           GCGI    + +HP F+ S+C F++R+D  V DFS+ KC+  ++
Sbjct: 158 GCGIDYIMVGHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLI 196

[Close]

BLAST of MS001996 vs. ExPASy Swiss-Prot
Match: Q42463 (Protein DCL, chloroplastic OS=Solanum lycopersicum OX=4081 GN=DCL PE=2 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 3.2e-05
Identity = 37/129 (28.68%), Postives = 63/129 (48.84%), Query Frame = 0


Query: 77  KVEKSVEEERMEKVEGDGKGDEKSGP----VSLGPKSFGSSVEMFDYFYKFLH--YWPAN 136
           ++E + E E + K E D +  +KSG     V    +    +V +  +    LH   +   
Sbjct: 73  ELETTSESEELVKEESDDEVGKKSGDGEGWVDWEDQILEDTVPLVGFVRMILHSGKYAIG 132

Query: 137 LNLNEYEQMVLLELLKKGHVEPEKKIGCGIHAFQIRNHPTFK-SKCYFLIREDESVDDFS 196
             L+   Q  +L+ L   H E +KKIG G+    +  HP F+ S+C F++R+D    DFS
Sbjct: 133 DRLSPDHQRTILQRLLPYHPECDKKIGPGVDYITVGYHPDFENSRCLFIVRKDGETVDFS 192

Query: 197 FRKCVDHIL 199
           + KC+  ++
Sbjct: 193 YWKCIKGLI 201

[Close]

BLAST of MS001996 vs. ExPASy TrEMBL
Match: A0A6J1C0K4 (protein EMBRYO DEFECTIVE 514 OS=Momordica charantia OX=3673 GN=LOC111007244 PE=4 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 8.6e-123
Identity = 237/238 (99.58%), Postives = 237/238 (99.58%), Query Frame = 0


Query: 1   MAEETTPQLAENDPEVTNSATEDMELETSEPGAEPKGLNGDSEANEAAPETNGDANSKRE 60
           MAEETTPQLAENDPEVTNSATEDMELETSEPGAEPKGLNGDSEANEAAPETNGDANSKRE
Sbjct: 1   MAEETTPQLAENDPEVTNSATEDMELETSEPGAEPKGLNGDSEANEAAPETNGDANSKRE 60

Query: 61  REEETADENIGEAKKPKVEKSVEEERMEKVEGDGKGDEKSGPVSLGPKSFGSSVEMFDYF 120
           REEETADENIGEAKKPKVEKSVEEERMEKVEGDGKGDEKSGPVSLGPKSFGSSVEMFDYF
Sbjct: 61  REEETADENIGEAKKPKVEKSVEEERMEKVEGDGKGDEKSGPVSLGPKSFGSSVEMFDYF 120

Query: 121 YKFLHYWPANLNLNEYEQMVLLELLKKGHVEPEKKIGCGIHAFQIRNHPTFKSKCYFLIR 180
           YKFLHYWPANLNLNEYEQMVLLELLKKGHVEPEKKIGCGIHAFQIRNHPTFKSKCYFLIR
Sbjct: 121 YKFLHYWPANLNLNEYEQMVLLELLKKGHVEPEKKIGCGIHAFQIRNHPTFKSKCYFLIR 180

Query: 181 EDESVDDFSFRKCVDHILPLPENLKVKSDANRALGGGKHRGGGRGGGGGRGRGGRSRN 239
           EDESVDDFSFRKCVDHILPLPENLKVKSDANRALGG KHRGGGRGGGGGRGRGGRSRN
Sbjct: 181 EDESVDDFSFRKCVDHILPLPENLKVKSDANRALGGCKHRGGGRGGGGGRGRGGRSRN 238

[Close]

BLAST of MS001996 vs. ExPASy TrEMBL
Match: A0A6J1F569 (protein EMBRYO DEFECTIVE 514-like OS=Cucurbita moschata OX=3662 GN=LOC111440949 PE=4 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 1.4e-93
Identity = 186/228 (81.58%), Postives = 203/228 (89.04%), Query Frame = 0


Query: 1   MAEETTPQLAENDPEVTNSATEDMELETSEPGAEPKGLNGDSEANEAAPETNGDANSKRE 60
           MAEET PQLAEN PEVTNSA EDMELE++    EPK   GDS+ANEAAP TNGDANSKRE
Sbjct: 1   MAEETIPQLAENGPEVTNSAAEDMELEST----EPKVSGGDSDANEAAPVTNGDANSKRE 60

Query: 61  REEETADENIGEAKKPKVEKSVEEERMEKVEGDGKGDEKSGPVSLGPKSFGSSVEMFDYF 120
           REEE AD++ GEAKKPKVEKSVEEER+EKVE DGKG+E+ GPVSLGPKSFGSSVE+FDYF
Sbjct: 61  REEEGADDHAGEAKKPKVEKSVEEERLEKVERDGKGEEEHGPVSLGPKSFGSSVELFDYF 120

Query: 121 YKFLHYWPANLNLNEYEQMVLLELLKKGHVEPEKKIGCGIHAFQIRNHPTFKSKCYFLIR 180
           Y FLHYWPANLN+N+YE MVLL+L+KKGHVEP+KKIGCG+ A QIR HP +KSKC+FLIR
Sbjct: 121 YNFLHYWPANLNVNQYELMVLLDLIKKGHVEPDKKIGCGVRACQIRYHPMWKSKCFFLIR 180

Query: 181 EDESVDDFSFRKCVDHILPLPENLKVKSDANRALGGGKHRGGGRGGGG 229
           ED S DDFSFRKCVDHILPLPENLK  SDANRALGGGKHRGGGRGGGG
Sbjct: 181 EDGSADDFSFRKCVDHILPLPENLKTISDANRALGGGKHRGGGRGGGG 224

[Close]

BLAST of MS001996 vs. ExPASy TrEMBL
Match: A0A6J1IBL1 (protein EMBRYO DEFECTIVE 514-like OS=Cucurbita maxima OX=3661 GN=LOC111471455 PE=4 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 2.1e-92
Identity = 184/228 (80.70%), Postives = 202/228 (88.60%), Query Frame = 0


Query: 1   MAEETTPQLAENDPEVTNSATEDMELETSEPGAEPKGLNGDSEANEAAPETNGDANSKRE 60
           MAEET PQLAEN PEVTNSA EDMELE++    EPK   GDS+ANEAAP TNGDANSKRE
Sbjct: 1   MAEETIPQLAENGPEVTNSAAEDMELEST----EPKVSGGDSDANEAAPVTNGDANSKRE 60

Query: 61  REEETADENIGEAKKPKVEKSVEEERMEKVEGDGKGDEKSGPVSLGPKSFGSSVEMFDYF 120
           REEE A + +GEAKKPKVEKSVEEER+EKVE DGKG+E+ GPVSLGPKSFGSSVE+F+YF
Sbjct: 61  REEEGAGDYVGEAKKPKVEKSVEEERLEKVERDGKGEEEHGPVSLGPKSFGSSVELFEYF 120

Query: 121 YKFLHYWPANLNLNEYEQMVLLELLKKGHVEPEKKIGCGIHAFQIRNHPTFKSKCYFLIR 180
           Y FLHYWPANLN+N+YE MVLL+L+KKGHVEP+KKIGCG+ A QIR HP +KSKC+FLIR
Sbjct: 121 YNFLHYWPANLNVNQYELMVLLDLIKKGHVEPDKKIGCGVRACQIRYHPMWKSKCFFLIR 180

Query: 181 EDESVDDFSFRKCVDHILPLPENLKVKSDANRALGGGKHRGGGRGGGG 229
           ED S DDFSFRKCVDHILPLPENLK  SDANRALGGGKHRGGGRGGGG
Sbjct: 181 EDGSADDFSFRKCVDHILPLPENLKTISDANRALGGGKHRGGGRGGGG 224

[Close]

BLAST of MS001996 vs. ExPASy TrEMBL
Match: A0A6J1JDH2 (protein EMBRYO DEFECTIVE 514-like OS=Cucurbita maxima OX=3661 GN=LOC111484822 PE=4 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 2.0e-87
Identity = 182/247 (73.68%), Postives = 211/247 (85.43%), Query Frame = 0


Query: 1   MAEETTPQLAENDPEVTNSATEDMELETSEPGAEPKGLNGDSEANEAAPETNGDANSKRE 60
           MAEET PQ A+N+ EVTN+  EDMELE +EPGAEPKG N   EANEA+  TNGDANSKRE
Sbjct: 1   MAEETIPQPADNNQEVTNA--EDMELENTEPGAEPKGSN--DEANEASKITNGDANSKRE 60

Query: 61  REEETADENIGEAKKPKVEKSVEEERMEKVEGDGKGDEKSGPVSLGPKSFGSSVEMFDYF 120
           REEE+AD ++ EAKK K+EKSVEE R+EK +GDGKG+E+ GPV++GPKSF SS E+FD+F
Sbjct: 61  REEESADGSVEEAKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHF 120

Query: 121 YKFLHYWPANLNLNEYEQMVLLELLKKGHVEPEKKIGCGIHAFQIRNHPTFKSKCYFLIR 180
           YKFLH+WPA LN+N+YE+M+LL+LLKKGH EPEKKIGCGIH+FQIRNHP +K+KC+FLIR
Sbjct: 121 YKFLHHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPMWKNKCFFLIR 180

Query: 181 EDESVDDFSFRKCVDHILPLPENLKVKSDANRALGGGKHRGGG---------RGGGGGRG 239
           EDESVDDFSFRKCVDHILPLPENLK +SDAN+A GGGKHRGGG          GGGGGRG
Sbjct: 181 EDESVDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGGGGSGGGRGGGGGGRG 240

[Close]

BLAST of MS001996 vs. ExPASy TrEMBL
Match: A0A5A7UQ03 (Protein DCL OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002450 PE=4 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 2.2e-86
Identity = 182/237 (76.79%), Postives = 201/237 (84.81%), Query Frame = 0


Query: 1   MAEETTPQLAENDPEVTNSATEDMELETSEPGAEPKGLNGDSEANEAAPETNGDANSKRE 60
           MAEET PQL   DPEVTNS TEDMELET+     PKG +  SEA EAAP  NGDANSKRE
Sbjct: 1   MAEETIPQL---DPEVTNSGTEDMELETT----VPKGSSDASEATEAAPVINGDANSKRE 60

Query: 61  REEETADENIGEAKKPKVEKSVEEERMEKVEGDGKGDEKSGPVSLGPKSFGSSVEMFDYF 120
           R EE+AD+ +G+ KK K+EKSVEEER+EK+ GDGK +E+  PVSLGPKSF SSVE+FDYF
Sbjct: 61  R-EESADDGVGDGKKQKIEKSVEEERVEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYF 120

Query: 121 YKFLHYWPANLNLNEYEQMVLLELLKKGHVEPEKKIGCGIHAFQIRNHPTFKSKCYFLIR 180
           YK LH WP NLN+N+YEQMVL++LLKKGH+EPEKKIGCGI AFQIR HP +KSKC+FLIR
Sbjct: 121 YKILHNWPINLNVNQYEQMVLVDLLKKGHLEPEKKIGCGIQAFQIRFHPVWKSKCFFLIR 180

Query: 181 EDESVDDFSFRKCVDHILPLPENLKVKSDANRALGGGKHRGGGRGGGGGRGRGGRSR 238
           EDES DDFSFRKCVDHILPLPEN K KSDANRALGGGKHRGGGRGGGGGRGRGG  R
Sbjct: 181 EDESADDFSFRKCVDHILPLPENFKAKSDANRALGGGKHRGGGRGGGGGRGRGGGGR 229

[Close]

BLAST of MS001996 vs. TAIR 10
Match: AT5G62440.1 (Protein of unknown function (DUF3223) )

HSP 1 Score: 196.4 bits (498), Expect = 2.5e-50
Identity = 119/223 (53.36%), Postives = 142/223 (63.68%), Query Frame = 0


Query: 15  EVTNSATEDMELETSEPGAEPKGLNGDSEANEAAPETNGDANSKREREEETADENIGEAK 74
           EV      DME+ET+ P AE                  GD   KREREE   +EN GE+K
Sbjct: 14  EVNPDQKVDMEVETATPKAE-----------------TGD--EKREREETEEEENGGESK 73

Query: 75  KPKVEKSVEEERMEKVEGDGKGDEKSGPVSLGPKSFGSSVEMFDYFYKFLHYWPANLNLN 134
           K KV +                +EKSGPV LGPK F +SV MFDYF KFLH+WP +L++N
Sbjct: 74  KQKVGE----------------EEKSGPVKLGPKEFVTSVAMFDYFVKFLHFWPTDLDVN 133

Query: 135 EYEQMVLLELLKKGHVEPEKKIGCGIHAFQIRNHPTFKSKCYFLIREDESVDDFSFRKCV 194
           +YE MVLL+L+KKGH EPEKKIG GI  FQ+R HP +KS+C+FL+RED++ DDFSFRKCV
Sbjct: 134 KYEHMVLLDLIKKGHSEPEKKIGGGIKTFQVRTHPMWKSRCFFLVREDDTADDFSFRKCV 193

Query: 195 DHILPLPENLKVKSDANRALGGGKHRGGGRGGGGGRGRGGRSR 238
           D ILPLPEN+K         GGG+  GGGR GG G GRGGR R
Sbjct: 194 DQILPLPENMKTPGANGNGHGGGRGGGGGRRGGRGGGRGGRFR 201

[Close]

BLAST of MS001996 vs. TAIR 10
Match: AT1G45230.1 (Protein of unknown function (DUF3223) )

HSP 1 Score: 54.3 bits (129), Expect = 1.6e-07
Identity = 50/163 (30.67%), Postives = 80/163 (49.08%), Query Frame = 0


Query: 53  GDANSKREREEETADENIGE------------AKKPKV--EKSVEEERMEKVEGDGKGDE 112
           GD+ S R R      E+ G              ++P++  E+S EEE  E+ E + +GDE
Sbjct: 38  GDSTSLRPRVRALRTESDGAKIGNSESYGSELLRRPRIASEESSEEEE-EEEEENSEGDE 97

Query: 113 KSGPVSLGPKSFGSSVEMFDYFYKFLH--YWPANLNLNEYEQMVLLELLKKGHVEPEKKI 172
               V    K    +V +  +    LH   +     L+   +  ++E+L   H E EKKI
Sbjct: 98  F---VDWEDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKKI 157

Query: 173 GCGIHAFQIRNHPTFK-SKCYFLIREDESVDDFSFRKCVDHIL 199
           GCGI    + +HP F+ S+C F++R+D  V DFS+ KC+  ++
Sbjct: 158 GCGIDYIMVGHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLI 196

[Close]

BLAST of MS001996 vs. TAIR 10
Match: AT1G45230.2 (Protein of unknown function (DUF3223) )

HSP 1 Score: 53.9 bits (128), Expect = 2.0e-07
Identity = 50/163 (30.67%), Postives = 80/163 (49.08%), Query Frame = 0


Query: 53  GDANSKREREEETADENIGE------------AKKPKV--EKSVEEERMEKVEGDGKGDE 112
           GD+ S R R      E+ G              ++P++  E+S EEE  E+ E + +GDE
Sbjct: 38  GDSTSLRPRVRALRTESDGAKIGNSESYGSELLRRPRIASEESSEEEE-EEEEENSEGDE 97

Query: 113 KSGPVSLGPKSFGSSVEMFDYFYKFLH--YWPANLNLNEYEQMVLLELLKKGHVEPEKKI 172
               V    K    +V +  +    LH   +     L+   +  ++E+L   H E EKKI
Sbjct: 98  F---VDWEDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKKI 157

Query: 173 GCGIHAFQIRNHPTFK-SKCYFLIREDESVDDFSFRKCVDHIL 199
           GCGI    + +HP F+ S+C F++R+D  V DFS+ KC+  ++
Sbjct: 158 GCGIDYIMVWHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLI 196

The following BLAST results are available for this feature:

BLAST of MS001996 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135230.1	1.8e-122	99.58	protein EMBRYO DEFECTIVE 514 [Momordica charantia]	[more]
XP_022933555.1	3.0e-93	81.58	protein EMBRYO DEFECTIVE 514-like [Cucurbita moschata]	[more]
KAG7021514.1	1.9e-92	81.30	Protein EMBRYO DEFECTIVE [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022972943.1	4.3e-92	80.70	protein EMBRYO DEFECTIVE 514-like [Cucurbita maxima]	[more]
XP_023529405.1	1.8e-90	79.82	protein EMBRYO DEFECTIVE 514-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS001996 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L557	3.6e-49	53.36	Protein EMBRYO DEFECTIVE 514 OS=Arabidopsis thaliana OX=3702 GN=EMB514 PE=1 SV=1	[more]
Q9C642	2.2e-06	30.67	Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 S...	[more]
Q42463	3.2e-05	28.68	Protein DCL, chloroplastic OS=Solanum lycopersicum OX=4081 GN=DCL PE=2 SV=1	[more]

BLAST of MS001996 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C0K4	8.6e-123	99.58	protein EMBRYO DEFECTIVE 514 OS=Momordica charantia OX=3673 GN=LOC111007244 PE=4...	[more]
A0A6J1F569	1.4e-93	81.58	protein EMBRYO DEFECTIVE 514-like OS=Cucurbita moschata OX=3662 GN=LOC111440949 ...	[more]
A0A6J1IBL1	2.1e-92	80.70	protein EMBRYO DEFECTIVE 514-like OS=Cucurbita maxima OX=3661 GN=LOC111471455 PE...	[more]
A0A6J1JDH2	2.0e-87	73.68	protein EMBRYO DEFECTIVE 514-like OS=Cucurbita maxima OX=3661 GN=LOC111484822 PE...	[more]
A0A5A7UQ03	2.2e-86	76.79	Protein DCL OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002450 P...	[more]

BLAST of MS001996 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62440.1	2.5e-50	53.36	Protein of unknown function (DUF3223)	[more]
AT1G45230.1	1.6e-07	30.67	Protein of unknown function (DUF3223)	[more]
AT1G45230.2	2.0e-07	30.67	Protein of unknown function (DUF3223)	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF11523	DUF3223	coord: 122..194 e-value: 7.9E-24 score: 84.2
None	No IPR available	GENE3D	3.10.450.40		coord: 98..197 e-value: 1.7E-25 score: 91.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 10..25
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 53..98
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..107
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 209..238
None	No IPR available	PANTHER	PTHR33415:SF12	PROTEIN EMBRYO DEFECTIVE 514	coord: 15..228
IPR044673	Protein DCL-like	PANTHER	PTHR33415	FAMILY NOT NAMED	coord: 15..228

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS001996.1	MS001996.1	mRNA