MS001971 (gene) Bitter gourd (TR) v1

Overview


Name	MS001971
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RNase H domain-containing protein
Location	scaffold30: 1378700 .. 1379020 (+)
RNA-Seq Expression	MS001971
Synteny	MS001971

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATGCGAATGGATTCTGACATTTGTGAAGGATTTCAAGGAAAGTAGCAGTCTTTGCCATGGATCGATTTCCACTTATTCGCCTCAAGTCAGCTCTGATAGCGACTCCCTGTCTGTTTGGTCTCCTCCCTCAATTGGGTGGGTGAAGATAAATGTGGATGCTGCATGTAAGGAAACTTTGTTTAGAACTGAAATTGGTGTTGTTGCCAGAGATGAAAGGGGTCAAATTATTGCGGCTGTTTCTCGAAGGGTAGAAGCTTACCACGATCCATTGATGGTCGAATCGCTGGCTTTGCTCGAAGGTTTCCATCTGGTGATGAGG

mRNA sequence

AAATGCGAATGGATTCTGACATTTGTGAAGGATTTCAAGGAAAGTAGCAGTCTTTGCCATGGATCGATTTCCACTTATTCGCCTCAAGTCAGCTCTGATAGCGACTCCCTGTCTGTTTGGTCTCCTCCCTCAATTGGGTGGGTGAAGATAAATGTGGATGCTGCATGTAAGGAAACTTTGTTTAGAACTGAAATTGGTGTTGTTGCCAGAGATGAAAGGGGTCAAATTATTGCGGCTGTTTCTCGAAGGGTAGAAGCTTACCACGATCCATTGATGGTCGAATCGCTGGCTTTGCTCGAAGGTTTCCATCTGGTGATGAGG

Coding sequence (CDS)

AAATGCGAATGGATTCTGACATTTGTGAAGGATTTCAAGGAAAGTAGCAGTCTTTGCCATGGATCGATTTCCACTTATTCGCCTCAAGTCAGCTCTGATAGCGACTCCCTGTCTGTTTGGTCTCCTCCCTCAATTGGGTGGGTGAAGATAAATGTGGATGCTGCATGTAAGGAAACTTTGTTTAGAACTGAAATTGGTGTTGTTGCCAGAGATGAAAGGGGTCAAATTATTGCGGCTGTTTCTCGAAGGGTAGAAGCTTACCACGATCCATTGATGGTCGAATCGCTGGCTTTGCTCGAAGGTTTCCATCTGGTGATGAGG

Protein sequence

KCEWILTFVKDFKESSSLCHGSISTYSPQVSSDSDSLSVWSPPSIGWVKINVDAACKETLFRTEIGVVARDERGQIIAAVSRRVEAYHDPLMVESLALLEGFHLVMR

Homology

[Close]

BLAST of MS001971 vs. NCBI nr
Match: XP_022145060.1 (uncharacterized protein LOC111014578 [Momordica charantia])

HSP 1 Score: 98.2 bits (243), Expect = 4.5e-17
Identity = 50/101 (49.50%), Postives = 64/101 (63.37%), Query Frame = 0


Query: 1   KCEWILTFVKDFKESSSLCHGSISTYSPQVSSDSDSLSVWSPPSIGWVKINVDAACKETL 60
           + +WILT+V+DF+       G         S+D    + WSPP  GW+KINVDAACK+  
Sbjct: 114 RSDWILTYVRDFQVRDVPSLGDFRIQEDASSNDRTIENYWSPPPAGWIKINVDAACKKHQ 173

Query: 61  FRTEIGVVARDERGQIIAAVSRRVEAYHDPLMVESLALLEG 102
           FRT IG+V R+E+GQI+AA S      HDPLM ESLAL +G
Sbjct: 174 FRTGIGIVCRNEKGQILAAAS------HDPLMAESLALFDG 208

[Close]

BLAST of MS001971 vs. NCBI nr
Match: XP_022142956.1 (uncharacterized protein LOC111012950 [Momordica charantia])

HSP 1 Score: 93.6 bits (231), Expect = 1.1e-15
Identity = 43/59 (72.88%), Postives = 50/59 (84.75%), Query Frame = 0


Query: 46  GWVKINVDAACKETLFRTEIGVVARDERGQIIAAVSRRVEAYHDPLMVESLALLEGFHL 105
           GW+KINVD ACK+  FRT IG+V R+E+GQI+AA SRR+EAYHDPLM ESLALLEG  L
Sbjct: 31  GWIKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAYHDPLMAESLALLEGLRL 89

[Close]

BLAST of MS001971 vs. NCBI nr
Match: XP_022154440.1 (uncharacterized protein LOC111021711 [Momordica charantia])

HSP 1 Score: 80.1 bits (196), Expect = 1.3e-11
Identity = 37/85 (43.53%), Postives = 57/85 (67.06%), Query Frame = 0


Query: 20  HGSISTYSPQVSSDSDSLSVWSPPSIGWVKINVDAACKETLFRTEIGVVARDERGQIIAA 79
           H     + P  +     ++ W+PPS  WVKINVD ACK +  RT IGVV ++++G+II A
Sbjct: 108 HERADIHLPNSNKAGKDVNYWAPPSPDWVKINVDTACKLSNSRTGIGVVCQNDKGEIIVA 167

Query: 80  VSRRVEAYHDPLMVESLALLEGFHL 105
           + R+++ +++PL VE+LA+LEG  L
Sbjct: 168 MIRKLDVFYEPLQVETLAILEGLWL 192

[Close]

BLAST of MS001971 vs. NCBI nr
Match: XP_042939410.1 (uncharacterized protein LOC122274438 [Carya illinoinensis])

HSP 1 Score: 65.9 bits (159), Expect = 2.5e-07
Identity = 30/63 (47.62%), Postives = 42/63 (66.67%), Query Frame = 0


Query: 39  VWSPPSIGWVKINVDAACKETLFRTEIGVVARDERGQIIAAVSRRVEAYHDPLMVESLAL 98
           +W PP  G +K+NVD A     FR+ IGVV RD+RGQ++ A S+  EA  DP+ +E +A+
Sbjct: 633 IWKPPPSGMLKLNVDGALFSDQFRSGIGVVLRDDRGQVLFAASKPEEALADPMEIELIAM 692

Query: 99  LEG 102
           L G
Sbjct: 693 LRG 695

[Close]

BLAST of MS001971 vs. NCBI nr
Match: KAB2597616.1 (hypothetical protein D8674_000536 [Pyrus ussuriensis x Pyrus communis])

HSP 1 Score: 62.0 bits (149), Expect = 3.6e-06
Identity = 33/102 (32.35%), Postives = 51/102 (50.00%), Query Frame = 0


Query: 6   LTFVKDFKESSSLCHGSISTYSPQVSSDSDSLSVWSPPSIGWVKINVDAACKETLFRTEI 65
           L F+K   +  S C                  S W+ P +G++K+NVD A  E+L   E+
Sbjct: 14  LPFLKSLSQDGSYCRAQALA------------SAWTKPPLGFLKVNVDGAWSESLKSGEV 73

Query: 66  GVVARDERGQIIAAVSRRVEAYHDPLMVESLALLEGFHLVMR 108
           GVV R E G  +AA ++  E    PL++E+ A+ EG  L ++
Sbjct: 74  GVVVRHEHGNFVAASTKTFEHVSSPLLIEAFAVREGLLLAVQ 103

[Close]

BLAST of MS001971 vs. ExPASy TrEMBL
Match: A0A6J1CTE3 (uncharacterized protein LOC111014578 OS=Momordica charantia OX=3673 GN=LOC111014578 PE=4 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 2.2e-17
Identity = 50/101 (49.50%), Postives = 64/101 (63.37%), Query Frame = 0


Query: 1   KCEWILTFVKDFKESSSLCHGSISTYSPQVSSDSDSLSVWSPPSIGWVKINVDAACKETL 60
           + +WILT+V+DF+       G         S+D    + WSPP  GW+KINVDAACK+  
Sbjct: 114 RSDWILTYVRDFQVRDVPSLGDFRIQEDASSNDRTIENYWSPPPAGWIKINVDAACKKHQ 173

Query: 61  FRTEIGVVARDERGQIIAAVSRRVEAYHDPLMVESLALLEG 102
           FRT IG+V R+E+GQI+AA S      HDPLM ESLAL +G
Sbjct: 174 FRTGIGIVCRNEKGQILAAAS------HDPLMAESLALFDG 208

[Close]

BLAST of MS001971 vs. ExPASy TrEMBL
Match: A0A6J1CNR4 (uncharacterized protein LOC111012950 OS=Momordica charantia OX=3673 GN=LOC111012950 PE=4 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 5.4e-16
Identity = 43/59 (72.88%), Postives = 50/59 (84.75%), Query Frame = 0


Query: 46  GWVKINVDAACKETLFRTEIGVVARDERGQIIAAVSRRVEAYHDPLMVESLALLEGFHL 105
           GW+KINVD ACK+  FRT IG+V R+E+GQI+AA SRR+EAYHDPLM ESLALLEG  L
Sbjct: 31  GWIKINVDVACKKHQFRTAIGIVCRNEKGQILAAASRRMEAYHDPLMAESLALLEGLRL 89

[Close]

BLAST of MS001971 vs. ExPASy TrEMBL
Match: A0A6J1DJL6 (uncharacterized protein LOC111021711 OS=Momordica charantia OX=3673 GN=LOC111021711 PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 6.2e-12
Identity = 37/85 (43.53%), Postives = 57/85 (67.06%), Query Frame = 0


Query: 20  HGSISTYSPQVSSDSDSLSVWSPPSIGWVKINVDAACKETLFRTEIGVVARDERGQIIAA 79
           H     + P  +     ++ W+PPS  WVKINVD ACK +  RT IGVV ++++G+II A
Sbjct: 108 HERADIHLPNSNKAGKDVNYWAPPSPDWVKINVDTACKLSNSRTGIGVVCQNDKGEIIVA 167

Query: 80  VSRRVEAYHDPLMVESLALLEGFHL 105
           + R+++ +++PL VE+LA+LEG  L
Sbjct: 168 MIRKLDVFYEPLQVETLAILEGLWL 192

[Close]

BLAST of MS001971 vs. ExPASy TrEMBL
Match: A0A5N5F3P3 (RNase H domain-containing protein OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_000536 PE=4 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.7e-06
Identity = 33/102 (32.35%), Postives = 51/102 (50.00%), Query Frame = 0


Query: 6   LTFVKDFKESSSLCHGSISTYSPQVSSDSDSLSVWSPPSIGWVKINVDAACKETLFRTEI 65
           L F+K   +  S C                  S W+ P +G++K+NVD A  E+L   E+
Sbjct: 14  LPFLKSLSQDGSYCRAQALA------------SAWTKPPLGFLKVNVDGAWSESLKSGEV 73

Query: 66  GVVARDERGQIIAAVSRRVEAYHDPLMVESLALLEGFHLVMR 108
           GVV R E G  +AA ++  E    PL++E+ A+ EG  L ++
Sbjct: 74  GVVVRHEHGNFVAASTKTFEHVSSPLLIEAFAVREGLLLAVQ 103

[Close]

BLAST of MS001971 vs. ExPASy TrEMBL
Match: A0A6P9EIJ2 (uncharacterized protein LOC118348883 OS=Juglans regia OX=51240 GN=LOC118348883 PE=4 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 3.9e-06
Identity = 28/65 (43.08%), Postives = 43/65 (66.15%), Query Frame = 0


Query: 37  LSVWSPPSIGWVKINVDAACKETLFRTEIGVVARDERGQIIAAVSRRVEAYHDPLMVESL 96
           +S W PP  G +K+NVD A      + ++G++ARD  G+++ AVS++ EA  +PL +E L
Sbjct: 322 ISQWQPPPQGSIKLNVDGAVFHAESKAKVGLIARDWEGKVLMAVSQKEEAVMEPLEIELL 381

Query: 97  ALLEG 102
           ALL G
Sbjct: 382 ALLRG 386

The following BLAST results are available for this feature:

BLAST of MS001971 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145060.1	4.5e-17	49.50	uncharacterized protein LOC111014578 [Momordica charantia]	[more]
XP_022142956.1	1.1e-15	72.88	uncharacterized protein LOC111012950 [Momordica charantia]	[more]
XP_022154440.1	1.3e-11	43.53	uncharacterized protein LOC111021711 [Momordica charantia]	[more]
XP_042939410.1	2.5e-07	47.62	uncharacterized protein LOC122274438 [Carya illinoinensis]	[more]
KAB2597616.1	3.6e-06	32.35	hypothetical protein D8674_000536 [Pyrus ussuriensis x Pyrus communis]	[more]

BLAST of MS001971 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS001971 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CTE3	2.2e-17	49.50	uncharacterized protein LOC111014578 OS=Momordica charantia OX=3673 GN=LOC111014...	[more]
A0A6J1CNR4	5.4e-16	72.88	uncharacterized protein LOC111012950 OS=Momordica charantia OX=3673 GN=LOC111012...	[more]
A0A6J1DJL6	6.2e-12	43.53	uncharacterized protein LOC111021711 OS=Momordica charantia OX=3673 GN=LOC111021...	[more]
A0A5N5F3P3	1.7e-06	32.35	RNase H domain-containing protein OS=Pyrus ussuriensis x Pyrus communis OX=24484...	[more]
A0A6P9EIJ2	3.9e-06	43.08	uncharacterized protein LOC118348883 OS=Juglans regia OX=51240 GN=LOC118348883 P...	[more]

BLAST of MS001971 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 51..105 e-value: 6.4E-7 score: 29.2
None	No IPR available	PANTHER	PTHR47074:SF37	PROTEIN, PUTATIVE-RELATED	coord: 33..104
None	No IPR available	PANTHER	PTHR47074	BNAC02G40300D PROTEIN	coord: 33..104

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS001971.1	MS001971.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity