MS001965 (gene) Bitter gourd (TR) v1

Overview


Name	MS001965
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	protein NUCLEAR FUSION DEFECTIVE 4-like
Location	scaffold30: 1335732 .. 1337870 (-)
RNA-Seq Expression	MS001965
Synteny	MS001965

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TGCAATTCAACGGAATTGGAAATGGAGACGCCCAGATTAAACAACAAATGGGTATCAACAGTTGCTAGCGTATGGATTCAATGCACCAGTGGGTCTCTCTACACCTTCTCCATCTACTCGCAAACCCTCAAGTCCACTCAGCGCTACGATCAGTCCACTTTGGACATTGTTTCTGTTTTCAAGGACATCGGTGTCAATTGCGGCGTCCTTTCAGGCTTTCTCTACTACTACGCCGCCGCTGATGGCGGTCGTCGTGGACCGTGGATTGTCCACTTGGCCGGTGCAATTCAGTGCTTCTTGGGTTATTTCCTTATCTGGGCTGCCGTTGCTGGGCTCTTCCCTCGGCCGCCGGTTCCCGCTATGTGCCTTTTCATGTTGGTGGCTGCTCATGCTCAGAGCTTCTTCAACACGGCTAACGTTGTCACTGGCGTTCGTAATTTTCCCAGTTATAGTGGGACGATTGTCGGAATCATGAAGGTAAAACTTATCGACGTTGAAATTCAATCGACATGACTATGGTTTTCGATAAACTTAGACATTATTTCATTTATATATCTTGAATACGTAATTATGACGTTCAGCCCTGATCTATGGAGTTCGTTTCGTCTGCAAATCAGATTGAGGAAGTTCAATTACATACGGAGTAGCGATGAGTATGAAGTTTTGAATTATGTTTTCTGAAGAAAACTATGATGGGTTGTGTCCAATACATAGCTTTCATATTTTATTTTCTCATAAATTTGTTCTGAAAAAAGAGCTTAGAACCATTATTTTGAGCACCTGAAGTTGGATTGCTAGTTTGTTGACCTTCAAATCTTTTATTAACCTTTAAAGAAGAAGAATTGTATCATCTTAGGTGATGCTCGCAATATTTTGTAGGTCGTACTTTCATTTGTCCTACTATTCTTTTGAAATCAAATTCGAATTTGAGTGAGGTCTTACTTGGTATGGTATTGGATCCTTGGGGATCTCACCTGAAAATTATTGGATTTTATTTGGACCCAATATAATTTTGTTTTTTTTTGTCAGGGGTTTCTTGGTTTGAGTGGAGCAATATTGATTCAAGTATACGAGACTATATTTGATGAACAGCCTACTTCGTTTATTCTGATGTTGGCACTGCTACCCACCCTTAATTCCTTATTGTTCATGTGGTTTGTGAGAATTCACAATACAAATGATGGAATTGAGAAAGAGCATCTCAATACCCTATCCATAATCACTCTGCTAGTTGCTATTTACCTTATGTTCAAGATAGTTCTAGAGCATATTTTCACCTTCCAATTCCCTCTACACGTTGCTGCATTTGTTCTACTTTTGATGTTGCTTGCATCTCCTCTATATATTGCAATCAGAGCCCAGCAGAGAGAATCCACTAATATTTTGCTCTCTTCTCTTACCGAGAGTGACCAACTGATTGGTCACTCCAATCAAGCGATCATGGATTTTGACAATGAATGGCCAAGGGAATCTGAAGAAAACCTTAACCTCTTGCAAGCTTTATACACCATAGACTTTTGGATTTTGTTTTTTGCAACAGCTTGCGGCATGGGAACAGGATTAGCTACAGTGAATAATATCAGCCAGATAGGATTATCTCTTGGTTACACAAGCTTGGAGATAAGTACTTTGGTTTCATTGTGGAGCATCTGGAACTTTTTTGGTCGTTTTGGAGCTGGATATGTATCAGATTATTTTCTACATGCAAGAGGATGGGCTAGGCCGTTATTCATGTTCCTCACTCTGACAACCATGAGCATTGGACATGTAGTGATTGCTTCTGGCCTGCCAGGTGCTCTTTTTGCTGGTTCGGTGATAGTGGGTGTTTGTTATGGTTCTCAGTGGTCACTAATGCCAACAATTACTTCTGAAATATTTGGTGTTGTACACATGGGTACTATATTCAATGCTATTACAATAGCGAGCCCTATTGGATCTTATTTATTTTCAGTTAGAGTTGTTGGGTATATATATGACAAGGAAGCATCAGGTGAAGGAGACACTTGTACTGGAACAAACTGCTTCATGTTATCATTTCTGATTATGGCTCTTGCCACCCTTCTGGGCTCTTGGGCAACCCTCGGATTATTTTTCTGGAGAAGAAGTTTCTACGATCAAGTGGTTTTTAGAAGGCTGCAA

mRNA sequence

TGCAATTCAACGGAATTGGAAATGGAGACGCCCAGATTAAACAACAAATGGGTATCAACAGTTGCTAGCGTATGGATTCAATGCACCAGTGGGTCTCTCTACACCTTCTCCATCTACTCGCAAACCCTCAAGTCCACTCAGCGCTACGATCAGTCCACTTTGGACATTGTTTCTGTTTTCAAGGACATCGGTGTCAATTGCGGCGTCCTTTCAGGCTTTCTCTACTACTACGCCGCCGCTGATGGCGGTCGTCGTGGACCGTGGATTGTCCACTTGGCCGGTGCAATTCAGTGCTTCTTGGGTTATTTCCTTATCTGGGCTGCCGTTGCTGGGCTCTTCCCTCGGCCGCCGGTTCCCGCTATGTGCCTTTTCATGTTGGTGGCTGCTCATGCTCAGAGCTTCTTCAACACGGCTAACGTTGTCACTGGCGTTCGTAATTTTCCCAGTTATAGTGGGACGATTGTCGGAATCATGAAGGGGTTTCTTGGTTTGAGTGGAGCAATATTGATTCAAGTATACGAGACTATATTTGATGAACAGCCTACTTCGTTTATTCTGATGTTGGCACTGCTACCCACCCTTAATTCCTTATTGTTCATGTGGTTTGTGAGAATTCACAATACAAATGATGGAATTGAGAAAGAGCATCTCAATACCCTATCCATAATCACTCTGCTAGTTGCTATTTACCTTATGTTCAAGATAGTTCTAGAGCATATTTTCACCTTCCAATTCCCTCTACACGTTGCTGCATTTGTTCTACTTTTGATGTTGCTTGCATCTCCTCTATATATTGCAATCAGAGCCCAGCAGAGAGAATCCACTAATATTTTGCTCTCTTCTCTTACCGAGAGTGACCAACTGATTGGTCACTCCAATCAAGCGATCATGGATTTTGACAATGAATGGCCAAGGGAATCTGAAGAAAACCTTAACCTCTTGCAAGCTTTATACACCATAGACTTTTGGATTTTGTTTTTTGCAACAGCTTGCGGCATGGGAACAGGATTAGCTACAGTGAATAATATCAGCCAGATAGGATTATCTCTTGGTTACACAAGCTTGGAGATAAGTACTTTGGTTTCATTGTGGAGCATCTGGAACTTTTTTGGTCGTTTTGGAGCTGGATATGTATCAGATTATTTTCTACATGCAAGAGGATGGGCTAGGCCGTTATTCATGTTCCTCACTCTGACAACCATGAGCATTGGACATGTAGTGATTGCTTCTGGCCTGCCAGGTGCTCTTTTTGCTGGTTCGGTGATAGTGGGTGTTTGTTATGGTTCTCAGTGGTCACTAATGCCAACAATTACTTCTGAAATATTTGGTGTTGTACACATGGGTACTATATTCAATGCTATTACAATAGCGAGCCCTATTGGATCTTATTTATTTTCAGTTAGAGTTGTTGGGTATATATATGACAAGGAAGCATCAGGTGAAGGAGACACTTGTACTGGAACAAACTGCTTCATGTTATCATTTCTGATTATGGCTCTTGCCACCCTTCTGGGCTCTTGGGCAACCCTCGGATTATTTTTCTGGAGAAGAAGTTTCTACGATCAAGTGGTTTTTAGAAGGCTGCAA

Coding sequence (CDS)

TGCAATTCAACGGAATTGGAAATGGAGACGCCCAGATTAAACAACAAATGGGTATCAACAGTTGCTAGCGTATGGATTCAATGCACCAGTGGGTCTCTCTACACCTTCTCCATCTACTCGCAAACCCTCAAGTCCACTCAGCGCTACGATCAGTCCACTTTGGACATTGTTTCTGTTTTCAAGGACATCGGTGTCAATTGCGGCGTCCTTTCAGGCTTTCTCTACTACTACGCCGCCGCTGATGGCGGTCGTCGTGGACCGTGGATTGTCCACTTGGCCGGTGCAATTCAGTGCTTCTTGGGTTATTTCCTTATCTGGGCTGCCGTTGCTGGGCTCTTCCCTCGGCCGCCGGTTCCCGCTATGTGCCTTTTCATGTTGGTGGCTGCTCATGCTCAGAGCTTCTTCAACACGGCTAACGTTGTCACTGGCGTTCGTAATTTTCCCAGTTATAGTGGGACGATTGTCGGAATCATGAAGGGGTTTCTTGGTTTGAGTGGAGCAATATTGATTCAAGTATACGAGACTATATTTGATGAACAGCCTACTTCGTTTATTCTGATGTTGGCACTGCTACCCACCCTTAATTCCTTATTGTTCATGTGGTTTGTGAGAATTCACAATACAAATGATGGAATTGAGAAAGAGCATCTCAATACCCTATCCATAATCACTCTGCTAGTTGCTATTTACCTTATGTTCAAGATAGTTCTAGAGCATATTTTCACCTTCCAATTCCCTCTACACGTTGCTGCATTTGTTCTACTTTTGATGTTGCTTGCATCTCCTCTATATATTGCAATCAGAGCCCAGCAGAGAGAATCCACTAATATTTTGCTCTCTTCTCTTACCGAGAGTGACCAACTGATTGGTCACTCCAATCAAGCGATCATGGATTTTGACAATGAATGGCCAAGGGAATCTGAAGAAAACCTTAACCTCTTGCAAGCTTTATACACCATAGACTTTTGGATTTTGTTTTTTGCAACAGCTTGCGGCATGGGAACAGGATTAGCTACAGTGAATAATATCAGCCAGATAGGATTATCTCTTGGTTACACAAGCTTGGAGATAAGTACTTTGGTTTCATTGTGGAGCATCTGGAACTTTTTTGGTCGTTTTGGAGCTGGATATGTATCAGATTATTTTCTACATGCAAGAGGATGGGCTAGGCCGTTATTCATGTTCCTCACTCTGACAACCATGAGCATTGGACATGTAGTGATTGCTTCTGGCCTGCCAGGTGCTCTTTTTGCTGGTTCGGTGATAGTGGGTGTTTGTTATGGTTCTCAGTGGTCACTAATGCCAACAATTACTTCTGAAATATTTGGTGTTGTACACATGGGTACTATATTCAATGCTATTACAATAGCGAGCCCTATTGGATCTTATTTATTTTCAGTTAGAGTTGTTGGGTATATATATGACAAGGAAGCATCAGGTGAAGGAGACACTTGTACTGGAACAAACTGCTTCATGTTATCATTTCTGATTATGGCTCTTGCCACCCTTCTGGGCTCTTGGGCAACCCTCGGATTATTTTTCTGGAGAAGAAGTTTCTACGATCAAGTGGTTTTTAGAAGGCTGCAA

Protein sequence

CNSTELEMETPRLNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQRYDQSTLDIVSVFKDIGVNCGVLSGFLYYYAAADGGRRGPWIVHLAGAIQCFLGYFLIWAAVAGLFPRPPVPAMCLFMLVAAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFDEQPTSFILMLALLPTLNSLLFMWFVRIHNTNDGIEKEHLNTLSIITLLVAIYLMFKIVLEHIFTFQFPLHVAAFVLLLMLLASPLYIAIRAQQRESTNILLSSLTESDQLIGHSNQAIMDFDNEWPRESEENLNLLQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLEISTLVSLWSIWNFFGRFGAGYVSDYFLHARGWARPLFMFLTLTTMSIGHVVIASGLPGALFAGSVIVGVCYGSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYLFSVRVVGYIYDKEASGEGDTCTGTNCFMLSFLIMALATLLGSWATLGLFFWRRSFYDQVVFRRLQ

Homology

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BLAST of MS001965 vs. NCBI nr
Match: XP_022135043.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Momordica charantia] >XP_022135044.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Momordica charantia] >XP_022135045.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Momordica charantia] >XP_022135046.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Momordica charantia] >XP_022135047.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Momordica charantia])

HSP 1 Score: 1018.8 bits (2633), Expect = 1.6e-293
Identity = 518/522 (99.23%), Postives = 518/522 (99.23%), Query Frame = 0


Query: 8   METPRLNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQRYDQSTLDIVSVFKDIGVNC 67
           METPRLNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQRYDQSTLDIVSVFKDIGVNC
Sbjct: 1   METPRLNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQRYDQSTLDIVSVFKDIGVNC 60

Query: 68  GVLSGFLYYYAAADGGRRGPWIVHLAGAIQCFLGYFLIWAAVAGLFPRPPVPAMCLFMLV 127
           GVLSGFLYYYAAADGGRRGPWIVHLAGAIQCFLGYFLIWAAVAGLFPRPPVPAMCLFMLV
Sbjct: 61  GVLSGFLYYYAAADGGRRGPWIVHLAGAIQCFLGYFLIWAAVAGLFPRPPVPAMCLFMLV 120

Query: 128 AAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFDEQPTSFILM 187
           AAHAQSFFNT NVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFDEQPTSFILM
Sbjct: 121 AAHAQSFFNTGNVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFDEQPTSFILM 180

Query: 188 LALLPTLNSLLFMWFVRIHNTNDGIEKEHLNTLSIITLLVAIYLMFKIVLEHIFTFQFPL 247
           LALLPTLNSLLFMWFVRIHNTNDGIEKEHLNTLSIITLLVAIYLMFKIVLEHIFTFQFPL
Sbjct: 181 LALLPTLNSLLFMWFVRIHNTNDGIEKEHLNTLSIITLLVAIYLMFKIVLEHIFTFQFPL 240

Query: 248 HVAAFVLLLMLLASPLYIAIRAQQRESTNILLSSLTESDQLIGHSNQAIMDFDNEWPRES 307
           HVAAFVLLLMLLASPLYIAIRAQQ ESTNILLSSLTESDQLIGHSNQAIMDFDNEWPRES
Sbjct: 241 HVAAFVLLLMLLASPLYIAIRAQQTESTNILLSSLTESDQLIGHSNQAIMDFDNEWPRES 300

Query: 308 EENLNLLQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLEISTLVSLWSIW 367
           EENLNLLQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLEISTLVSLWSIW
Sbjct: 301 EENLNLLQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLEISTLVSLWSIW 360

Query: 368 NFFGRFGAGYVSDYFLHARGWARPLFMFLTLTTMSIGHVVIASGLPGALFAGSVIVGVCY 427
           NFFGRFGAGYVSDYFLHARGWARPLFMFLTLT MSIGHVVIASGLPGALFAGSVIVGVCY
Sbjct: 361 NFFGRFGAGYVSDYFLHARGWARPLFMFLTLTIMSIGHVVIASGLPGALFAGSVIVGVCY 420

Query: 428 GSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYLFSVRVVGYIYDKEASGEGDTCTG 487
           GSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYLFSVRVVGYIYDKEASGEGDTCTG
Sbjct: 421 GSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYLFSVRVVGYIYDKEASGEGDTCTG 480

Query: 488 TNCFMLSFLIMALATLLGSWATLGLFFWRRSFYDQVVFRRLQ 530
           TNCFMLSFLIMALATLLGSWA LGLFFWRRSFYDQVVFRRLQ
Sbjct: 481 TNCFMLSFLIMALATLLGSWAALGLFFWRRSFYDQVVFRRLQ 522

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BLAST of MS001965 vs. NCBI nr
Match: XP_038879767.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 917.9 bits (2371), Expect = 3.9e-263
Identity = 471/525 (89.71%), Postives = 489/525 (93.14%), Query Frame = 0


Query: 5   ELEMETPRLNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQRYDQSTLDIVSVFKDIG 64
           ELE ET RLNNKWVSTVASVWIQCTSGSLYTFSIYSQ LKSTQ YDQSTLDIVSVFKDIG
Sbjct: 2   ELESETSRLNNKWVSTVASVWIQCTSGSLYTFSIYSQILKSTQEYDQSTLDIVSVFKDIG 61

Query: 65  VNCGVLSGFLYYYAAADGGRRGPWIVHLAGAIQCFLGYFLIWAAVAGLFPRPPVPAMCLF 124
           VNCGVL+GFLYYYA ADG R GPWIVHLAGAIQCFLGYF IWAAV+G+FPRP VPAMCLF
Sbjct: 62  VNCGVLAGFLYYYATADGNRCGPWIVHLAGAIQCFLGYFFIWAAVSGVFPRPSVPAMCLF 121

Query: 125 MLVAAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFDEQPTSF 184
           MLVAAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIF+ QPTSF
Sbjct: 122 MLVAAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFNGQPTSF 181

Query: 185 ILMLALLPTLNSLLFMWFVRIHNTNDGIEKEHLNTLSIITLLVAIYLMFKIVLEHIFTFQ 244
           +LMLALLPT NSLL M FVRIH+ +DGIEKEHLNTLSIITLLVA YLM KIVLEHIFTFQ
Sbjct: 182 LLMLALLPTFNSLLCMSFVRIHHADDGIEKEHLNTLSIITLLVATYLMIKIVLEHIFTFQ 241

Query: 245 FPLHVAAFVLLLMLLASPLYIAIRAQQRESTNILLSSLTESDQLIGHSNQAIMDFDNEWP 304
           F LHVA FVLLLMLLASPLYIAIRAQQRES  IL    TESDQLIG SNQ  +DFD+E  
Sbjct: 242 FSLHVATFVLLLMLLASPLYIAIRAQQRESKRILHPCFTESDQLIGCSNQETLDFDHEQR 301

Query: 305 RESEENLNLLQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLEISTLVSLW 364
           RESEE+LNL+QALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYT+LEI+TLVSLW
Sbjct: 302 RESEESLNLVQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTNLEINTLVSLW 361

Query: 365 SIWNFFGRFGAGYVSDYFLHARGWARPLFMFLTLTTMSIGHVVIASGLPGALFAGSVIVG 424
           SIWNFFGRFGAGYVSDYFLH+ GWARPLFMF+TLTTMSIGHVVIASGLPGALFAGSV+VG
Sbjct: 362 SIWNFFGRFGAGYVSDYFLHSYGWARPLFMFITLTTMSIGHVVIASGLPGALFAGSVVVG 421

Query: 425 VCYGSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYLFSVRVVGYIYDKEASGEGDT 484
           VCYGSQWSLMPTITSEIFGVVHMGTIFNAIT+ASP+GSYLFSVRVVGYIYDKEASGEGDT
Sbjct: 422 VCYGSQWSLMPTITSEIFGVVHMGTIFNAITVASPVGSYLFSVRVVGYIYDKEASGEGDT 481

Query: 485 CTGTNCFMLSFLIMALATLLGSWATLGLFFWRRSFYDQVVFRRLQ 530
           CTGT CFMLSF IMA ATLLGS A LGLFFWRRSFYDQVV RRLQ
Sbjct: 482 CTGTYCFMLSFFIMASATLLGSLAALGLFFWRRSFYDQVVIRRLQ 526

[Close]

BLAST of MS001965 vs. NCBI nr
Match: XP_023530012.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo] >XP_023530013.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo] >XP_023530014.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo] >XP_023530015.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo] >XP_023530016.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 910.2 bits (2351), Expect = 8.2e-261
Identity = 460/522 (88.12%), Postives = 486/522 (93.10%), Query Frame = 0


Query: 8   METPRLNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQRYDQSTLDIVSVFKDIGVNC 67
           ME  R NNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQ YDQSTLDIVSVFKDIGVNC
Sbjct: 1   MEISRFNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQGYDQSTLDIVSVFKDIGVNC 60

Query: 68  GVLSGFLYYYAAADGGRRGPWIVHLAGAIQCFLGYFLIWAAVAGLFPRPPVPAMCLFMLV 127
           GVL+GFLYYYA ADGG   PWIVHLAGAIQCFLGYFL+WAAVAG+FPRPP+PAMC FMLV
Sbjct: 61  GVLAGFLYYYATADGGGSRPWIVHLAGAIQCFLGYFLMWAAVAGVFPRPPLPAMCFFMLV 120

Query: 128 AAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFDEQPTSFILM 187
           AAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIF+EQPTSF+LM
Sbjct: 121 AAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFNEQPTSFLLM 180

Query: 188 LALLPTLNSLLFMWFVRIHNTNDGIEKEHLNTLSIITLLVAIYLMFKIVLEHIFTFQFPL 247
           LALLPTLNSLLFMWFVRIHN +D ++K+HLN+LSI+TL +A YLM KIVLEHIFTFQFPL
Sbjct: 181 LALLPTLNSLLFMWFVRIHNADDEVDKKHLNSLSIVTLFLATYLMLKIVLEHIFTFQFPL 240

Query: 248 HVAAFVLLLMLLASPLYIAIRAQQRESTNILLSSLTESDQLIGHSNQAIMDFDNEWPRES 307
            VA+F+LLL+LLASPLY+AIRAQQRES  IL  S+TESDQLI  SNQ   DFDNE  RES
Sbjct: 241 QVASFILLLILLASPLYVAIRAQQRESRKILHPSVTESDQLISRSNQESEDFDNERRRES 300

Query: 308 EENLNLLQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLEISTLVSLWSIW 367
           EE+LNL QALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLE + LVSLWSIW
Sbjct: 301 EESLNLFQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLETTILVSLWSIW 360

Query: 368 NFFGRFGAGYVSDYFLHARGWARPLFMFLTLTTMSIGHVVIASGLPGALFAGSVIVGVCY 427
           NFFGR GAGYVSDYFLHA+GWARPLFMF+TL TMSIGHVVIASGLPGALFAGSV+VGVCY
Sbjct: 361 NFFGRLGAGYVSDYFLHAKGWARPLFMFITLATMSIGHVVIASGLPGALFAGSVLVGVCY 420

Query: 428 GSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYLFSVRVVGYIYDKEASGEGDTCTG 487
           GSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSY+FSVRV+GY+YDKEASGEGDTCTG
Sbjct: 421 GSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYIFSVRVIGYMYDKEASGEGDTCTG 480

Query: 488 TNCFMLSFLIMALATLLGSWATLGLFFWRRSFYDQVVFRRLQ 530
             CFMLSFLIMA ATLLGS A LGLFFWRRSFYDQVVFRRLQ
Sbjct: 481 AYCFMLSFLIMAFATLLGSLAALGLFFWRRSFYDQVVFRRLQ 522

[Close]

BLAST of MS001965 vs. NCBI nr
Match: XP_022972552.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022972555.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022972556.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022972560.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022972570.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022972580.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022972589.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022972596.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022972604.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 907.9 bits (2345), Expect = 4.1e-260
Identity = 458/522 (87.74%), Postives = 485/522 (92.91%), Query Frame = 0


Query: 8   METPRLNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQRYDQSTLDIVSVFKDIGVNC 67
           ME    NNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQ YDQSTLDIVSVFKDIGVNC
Sbjct: 1   MEISSFNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQGYDQSTLDIVSVFKDIGVNC 60

Query: 68  GVLSGFLYYYAAADGGRRGPWIVHLAGAIQCFLGYFLIWAAVAGLFPRPPVPAMCLFMLV 127
           GVL+GFLYYYA ADGG   PWIVHLAGAIQCFLGYFL+WAAVAG+FPRPP+P MC FMLV
Sbjct: 61  GVLAGFLYYYATADGGGSRPWIVHLAGAIQCFLGYFLMWAAVAGVFPRPPLPTMCFFMLV 120

Query: 128 AAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFDEQPTSFILM 187
           AAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIF+EQPTSF+LM
Sbjct: 121 AAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFNEQPTSFLLM 180

Query: 188 LALLPTLNSLLFMWFVRIHNTNDGIEKEHLNTLSIITLLVAIYLMFKIVLEHIFTFQFPL 247
           LALLPTLNSLLFMWFVRIHN +DG+EK+HLN+LSI+TL +A YLM KIVLEHIFTFQFPL
Sbjct: 181 LALLPTLNSLLFMWFVRIHNADDGVEKKHLNSLSIVTLFLATYLMLKIVLEHIFTFQFPL 240

Query: 248 HVAAFVLLLMLLASPLYIAIRAQQRESTNILLSSLTESDQLIGHSNQAIMDFDNEWPRES 307
            VA+F+LLL+LLASPLY+AIRAQQRES  IL  S+TESDQLI  SNQ   +FDNE  RES
Sbjct: 241 QVASFILLLILLASPLYVAIRAQQRESRKILHPSVTESDQLISRSNQESEEFDNERRRES 300

Query: 308 EENLNLLQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLEISTLVSLWSIW 367
           EE+LNL QALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLE + LVSLWSIW
Sbjct: 301 EESLNLFQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLETTILVSLWSIW 360

Query: 368 NFFGRFGAGYVSDYFLHARGWARPLFMFLTLTTMSIGHVVIASGLPGALFAGSVIVGVCY 427
           NFFGR GAGYVSDYFLHA+GWARPLFMF+TL TMSIGHVVIASGLPGALFAGSV+VGVCY
Sbjct: 361 NFFGRLGAGYVSDYFLHAKGWARPLFMFITLATMSIGHVVIASGLPGALFAGSVLVGVCY 420

Query: 428 GSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYLFSVRVVGYIYDKEASGEGDTCTG 487
           GSQWSLMPTITSEIFGVVHMGTIFNAITIASP+GSY+FSVRV+GY+YDKEASGEG TCTG
Sbjct: 421 GSQWSLMPTITSEIFGVVHMGTIFNAITIASPVGSYIFSVRVIGYMYDKEASGEGGTCTG 480

Query: 488 TNCFMLSFLIMALATLLGSWATLGLFFWRRSFYDQVVFRRLQ 530
           T CFMLSFLIMA ATLLGS A LGLFFWRRSFYDQVVFRRLQ
Sbjct: 481 TYCFMLSFLIMAFATLLGSLAALGLFFWRRSFYDQVVFRRLQ 522

[Close]

BLAST of MS001965 vs. NCBI nr
Match: XP_004138144.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >XP_031740770.1 protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >XP_031740779.1 protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >XP_031740781.1 protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN63638.1 hypothetical protein Csa_013402 [Cucumis sativus])

HSP 1 Score: 902.9 bits (2332), Expect = 1.3e-258
Identity = 460/525 (87.62%), Postives = 479/525 (91.24%), Query Frame = 0


Query: 5   ELEMETPRLNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQRYDQSTLDIVSVFKDIG 64
           ELE ET  L NKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQ YDQSTLDIVSVFKDIG
Sbjct: 2   ELESETSSLKNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQGYDQSTLDIVSVFKDIG 61

Query: 65  VNCGVLSGFLYYYAAADGGRRGPWIVHLAGAIQCFLGYFLIWAAVAGLFPRPPVPAMCLF 124
           VNCGVL+GFLYY+A A GGR GPWIVH AGAIQCFLGYF IWAAV G+ PRPPVP MCLF
Sbjct: 62  VNCGVLAGFLYYFATAHGGRPGPWIVHFAGAIQCFLGYFFIWAAVYGVLPRPPVPVMCLF 121

Query: 125 MLVAAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFDEQPTSF 184
           MLVAAHAQSFFNTANVVTGVRNFP YSGTIVGIMKGFLGLSGAILIQ YETIF+ QPTSF
Sbjct: 122 MLVAAHAQSFFNTANVVTGVRNFPRYSGTIVGIMKGFLGLSGAILIQTYETIFNGQPTSF 181

Query: 185 ILMLALLPTLNSLLFMWFVRIHNTNDGIEKEHLNTLSIITLLVAIYLMFKIVLEHIFTFQ 244
           +LMLALLPTLNSLL MWFVRIH+ +DGIEKEHLNTLSIITL+VA YLM KIVLEHIFTFQ
Sbjct: 182 LLMLALLPTLNSLLCMWFVRIHHVDDGIEKEHLNTLSIITLVVATYLMIKIVLEHIFTFQ 241

Query: 245 FPLHVAAFVLLLMLLASPLYIAIRAQQRESTNILLSSLTESDQLIGHSNQAIMDFDNEWP 304
           FPLHVA F+LLLMLLASPLYIAIRAQ RES  IL  S TESDQLIG  NQ   DFD+E  
Sbjct: 242 FPLHVATFILLLMLLASPLYIAIRAQPRESRRILHPSFTESDQLIGRHNQETSDFDHERG 301

Query: 305 RESEENLNLLQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLEISTLVSLW 364
           RESEE+L L QALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTS EI+TLVSLW
Sbjct: 302 RESEESLTLFQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSSEINTLVSLW 361

Query: 365 SIWNFFGRFGAGYVSDYFLHARGWARPLFMFLTLTTMSIGHVVIASGLPGALFAGSVIVG 424
           SIWNFFGRFGAGYVSDY+LHA+GWARPLFMF+TL TMSIGHVVIASGLPGALFAGS++VG
Sbjct: 362 SIWNFFGRFGAGYVSDYYLHAKGWARPLFMFITLMTMSIGHVVIASGLPGALFAGSIVVG 421

Query: 425 VCYGSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYLFSVRVVGYIYDKEASGEGDT 484
           VCYGSQWSLMPTITSEIFGVVHMGTIFNAIT+ASP+GSYLFSVRVVGYIYDKEAS EGDT
Sbjct: 422 VCYGSQWSLMPTITSEIFGVVHMGTIFNAITVASPVGSYLFSVRVVGYIYDKEASSEGDT 481

Query: 485 CTGTNCFMLSFLIMALATLLGSWATLGLFFWRRSFYDQVVFRRLQ 530
           C GT CFMLSF IMA ATLLGS A LGLFFWRRSFYDQVV RRLQ
Sbjct: 482 CIGTYCFMLSFFIMAFATLLGSLAALGLFFWRRSFYDQVVVRRLQ 526

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BLAST of MS001965 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 7.5e-31
Identity = 137/542 (25.28%), Postives = 243/542 (44.83%), Query Frame = 0


Query: 16  KWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQRYDQSTLDIVSVFKDIGVNCGVLSGFLY 75
           KW   VA++WIQ ++G+ + FS YS  LKS     Q  L+ ++V  D+G   G  SG   
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 76  YYAAADGGRRGPWIVHLAGAIQCFLGYFLIWAAVAGLFPRPPVPAMCLFMLVAAHAQSFF 135
            Y           +V  A A   F+GY + W  +  +    P   + L  L+A  +  +F
Sbjct: 103 GYFPLS-------VVLFAAAAMGFVGYGVQWLVITNIITL-PYSLVFLCCLLAGLSICWF 162

Query: 136 NTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFDEQPTSFILMLALLPTLN 195
           NTA  +  +R+FP+     + +   F G+S A+    +  I       ++L+ +L+P + 
Sbjct: 163 NTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVV 222

Query: 196 SLLFMWFV----RIHNTNDGIEKEHLNTLSIITLLVAIYLMFKIVLEHIFTFQFPLH-VA 255
           S   ++ V     +  T D   + H + +  I  ++A+   F ++L    T    L+ + 
Sbjct: 223 SFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIG 282

Query: 256 AFVLLLMLLASPLYIAIR---------AQQRESTNILLSSL-------TESDQLIGHSNQ 315
           A VLL+  L +PL +  R             ES+  ++ ++       T      G+ + 
Sbjct: 283 AVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHM 342

Query: 316 AIMDFDNEWPRESEENLNLLQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTS 375
                 N      E +  LL  +  ++FW+ + A  CG   GL   NN+ QI  SLG  S
Sbjct: 343 GTAKEGNTVRLGDEHSFRLL--ISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNS 402

Query: 376 LEISTLVSLWSIWNFFGRFGAGYVSDYFLHAR------GWARPLFMFLTLTTMSIGHVVI 435
              +TLV+++S ++FFGR  +   +  F+H R      GW     + L  T ++   + +
Sbjct: 403 ---TTLVTIYSSFSFFGRLLS--AAPDFMHKRFRLTRTGW---FAIALLPTPIAFFLLAV 462

Query: 436 ASGLPGALFAGSVIVGVCYGSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYLFSVR 495
           +S    AL   + ++G+  G  ++   +ITS++FG   +G   N +    PIGS L+   
Sbjct: 463 SSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGY- 522

Query: 496 VVGYIYDKEAS-------GEGDTCTGTNCFMLSFLIMALATLLGSWATLGLFFWRRSFYD 524
           +   IY+  AS        +   C G +C+  +F+     ++LG  ++L L+   +  Y 
Sbjct: 523 IAASIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVYH 565

[Close]

BLAST of MS001965 vs. ExPASy Swiss-Prot
Match: Q6CGU8 (Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 2.9e-06
Identity = 43/176 (24.43%), Postives = 91/176 (51.70%), Query Frame = 0


Query: 354 SLEISTLVSLWSIWNFFGRFGAGYVSDYFLHARGWARPLFMFLTLTTMSIGHVVIASGL- 413
           S   ST VSL++ ++   R   G+ S+        +RP+ + +     +  H+++ SG+ 
Sbjct: 309 SPSFSTHVSLFATFSTVSRLVVGFSSEAM--ESHVSRPVLLSVIALVAACIHLMVPSGIF 368

Query: 414 ---PGALFAG--SVIVGVCYGSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYLFSV 473
                A +    +++ G  YGS ++L+PTI ++++G+ ++GTI+ +  +A  +GS  + +
Sbjct: 369 TVFDNAKYFSVVTIVNGFSYGSSFTLVPTIVTKVWGIANLGTIWGSFILALAVGSLGYGL 428

Query: 474 RVVGYIYDKEAS-GEG---DTCTGTNCFMLSFLIMALATLLGSWAT--LGLFFWRR 518
            +   +YD  +  G G     C+G +C+ L+F+I        + A   + +F W++
Sbjct: 429 -LFAKVYDAASEVGVGSMSQVCSGVHCYGLTFVITGTGLAFAAAAVFFIWVFMWKK 481

[Close]

BLAST of MS001965 vs. ExPASy Swiss-Prot
Match: Q07376 (Probable transporter MCH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MCH1 PE=1 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 4.6e-04
Identity = 40/183 (21.86%), Postives = 84/183 (45.90%), Query Frame = 0


Query: 337 LATVNNISQIGLSLGYTSLEISTLVSLWSIWNFFGRFGAGYVSDYFLHAR---GWARPLF 396
           +A + +++ + + L   +L  + L+S +++ + F R   G V+D+F   +    W    F
Sbjct: 299 IANMGSLTNLLVQLDAPTLS-TKLLSTYALSSTFTRLLTGIVADFFAKKKISIKWILLTF 358

Query: 397 MFLTLTTMSIGHVVIASGLPGALFAGSVIVGVCYGSQWSLMPTITSEIFGVVHMGTIFNA 456
           + L +        + +S  P  L     +VG+ YG  +++ PT+   ++G    GT++ +
Sbjct: 359 LSLGVCAQLFLLKMTSSASPWGLVPTGSLVGIVYGGLFTVYPTLVLLVWGERSFGTVYGS 418

Query: 457 ITIASPIGSYLFSVRVVGYIYDKEASGEGDTCTGTNCFMLSFLIMALATLLGSWATLGLF 516
           + IA  IGS +F + +    YD      G      +C    +   ++A ++ S     + 
Sbjct: 419 LLIAPAIGSMIFCM-LYAKFYDSRCMSGGGDLRNPSCISAVYKYSSIAFVV-SAVLSAVV 478

[Close]

BLAST of MS001965 vs. ExPASy Swiss-Prot
Match: Q6FWD4 (Probable transporter MCH1 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 7.8e-04
Identity = 58/251 (23.11%), Postives = 112/251 (44.62%), Query Frame = 0


Query: 251 AFVLLLMLLASPLYIAIR--AQQRESTNILLSSLTESDQLIGHSNQAIMDF--DNEWPR- 310
           AF  +  ++   ++IA    AQ +   +I    + E D++   S+  + +   DNE  R 
Sbjct: 205 AFAWIYTVIGVMIWIATSKVAQIQHEADI----MEEQDRMAEPSDNDVENHYDDNEQSRL 264

Query: 311 ---ESEENLNLLQALYTIDFWILFFATACGMG---TGLATVNNISQIGLSLGYTSLEIST 370
                 + + L++       +I      C +G     +A + +++ + L+ G+     S 
Sbjct: 265 LHATHAQQMTLMKVFRDPVLYIFGATIFCALGPLEMFIANMGSLTNV-LAGGHEPAMSSA 324

Query: 371 LVSLWSIWNFFGRFGAGYVSDYFLHAR---GWARPLFMFLTLTTMSIGHVVIASGLPGA- 430
           L+S++++ +   R G G   DYF   +    W   LF+ + L T    +++  S L  + 
Sbjct: 325 LLSIYALTSTLTRLGTGLTVDYFNKRQLSVKWILLLFLVVGLVTQGKIYMLSMSSLDHSH 384

Query: 431 -------LFAGSVIVGVCYGSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYLFSVR 480
                  LF   ++ G+ YG  +++ PTIT  ++G    GT +  + IA  +GS L S  
Sbjct: 385 MVTINRKLFYIGIMQGIAYGGLFTIYPTITLMVWGEKMFGTAYGTLMIAPALGSAL-SCL 444

[Close]

BLAST of MS001965 vs. ExPASy TrEMBL
Match: A0A6J1C1J4 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Momordica charantia OX=3673 GN=LOC111007132 PE=4 SV=1)

HSP 1 Score: 1018.8 bits (2633), Expect = 7.9e-294
Identity = 518/522 (99.23%), Postives = 518/522 (99.23%), Query Frame = 0


Query: 8   METPRLNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQRYDQSTLDIVSVFKDIGVNC 67
           METPRLNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQRYDQSTLDIVSVFKDIGVNC
Sbjct: 1   METPRLNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQRYDQSTLDIVSVFKDIGVNC 60

Query: 68  GVLSGFLYYYAAADGGRRGPWIVHLAGAIQCFLGYFLIWAAVAGLFPRPPVPAMCLFMLV 127
           GVLSGFLYYYAAADGGRRGPWIVHLAGAIQCFLGYFLIWAAVAGLFPRPPVPAMCLFMLV
Sbjct: 61  GVLSGFLYYYAAADGGRRGPWIVHLAGAIQCFLGYFLIWAAVAGLFPRPPVPAMCLFMLV 120

Query: 128 AAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFDEQPTSFILM 187
           AAHAQSFFNT NVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFDEQPTSFILM
Sbjct: 121 AAHAQSFFNTGNVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFDEQPTSFILM 180

Query: 188 LALLPTLNSLLFMWFVRIHNTNDGIEKEHLNTLSIITLLVAIYLMFKIVLEHIFTFQFPL 247
           LALLPTLNSLLFMWFVRIHNTNDGIEKEHLNTLSIITLLVAIYLMFKIVLEHIFTFQFPL
Sbjct: 181 LALLPTLNSLLFMWFVRIHNTNDGIEKEHLNTLSIITLLVAIYLMFKIVLEHIFTFQFPL 240

Query: 248 HVAAFVLLLMLLASPLYIAIRAQQRESTNILLSSLTESDQLIGHSNQAIMDFDNEWPRES 307
           HVAAFVLLLMLLASPLYIAIRAQQ ESTNILLSSLTESDQLIGHSNQAIMDFDNEWPRES
Sbjct: 241 HVAAFVLLLMLLASPLYIAIRAQQTESTNILLSSLTESDQLIGHSNQAIMDFDNEWPRES 300

Query: 308 EENLNLLQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLEISTLVSLWSIW 367
           EENLNLLQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLEISTLVSLWSIW
Sbjct: 301 EENLNLLQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLEISTLVSLWSIW 360

Query: 368 NFFGRFGAGYVSDYFLHARGWARPLFMFLTLTTMSIGHVVIASGLPGALFAGSVIVGVCY 427
           NFFGRFGAGYVSDYFLHARGWARPLFMFLTLT MSIGHVVIASGLPGALFAGSVIVGVCY
Sbjct: 361 NFFGRFGAGYVSDYFLHARGWARPLFMFLTLTIMSIGHVVIASGLPGALFAGSVIVGVCY 420

Query: 428 GSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYLFSVRVVGYIYDKEASGEGDTCTG 487
           GSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYLFSVRVVGYIYDKEASGEGDTCTG
Sbjct: 421 GSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYLFSVRVVGYIYDKEASGEGDTCTG 480

Query: 488 TNCFMLSFLIMALATLLGSWATLGLFFWRRSFYDQVVFRRLQ 530
           TNCFMLSFLIMALATLLGSWA LGLFFWRRSFYDQVVFRRLQ
Sbjct: 481 TNCFMLSFLIMALATLLGSWAALGLFFWRRSFYDQVVFRRLQ 522

[Close]

BLAST of MS001965 vs. ExPASy TrEMBL
Match: A0A6J1I6E1 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111471088 PE=4 SV=1)

HSP 1 Score: 907.9 bits (2345), Expect = 2.0e-260
Identity = 458/522 (87.74%), Postives = 485/522 (92.91%), Query Frame = 0


Query: 8   METPRLNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQRYDQSTLDIVSVFKDIGVNC 67
           ME    NNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQ YDQSTLDIVSVFKDIGVNC
Sbjct: 1   MEISSFNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQGYDQSTLDIVSVFKDIGVNC 60

Query: 68  GVLSGFLYYYAAADGGRRGPWIVHLAGAIQCFLGYFLIWAAVAGLFPRPPVPAMCLFMLV 127
           GVL+GFLYYYA ADGG   PWIVHLAGAIQCFLGYFL+WAAVAG+FPRPP+P MC FMLV
Sbjct: 61  GVLAGFLYYYATADGGGSRPWIVHLAGAIQCFLGYFLMWAAVAGVFPRPPLPTMCFFMLV 120

Query: 128 AAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFDEQPTSFILM 187
           AAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIF+EQPTSF+LM
Sbjct: 121 AAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFNEQPTSFLLM 180

Query: 188 LALLPTLNSLLFMWFVRIHNTNDGIEKEHLNTLSIITLLVAIYLMFKIVLEHIFTFQFPL 247
           LALLPTLNSLLFMWFVRIHN +DG+EK+HLN+LSI+TL +A YLM KIVLEHIFTFQFPL
Sbjct: 181 LALLPTLNSLLFMWFVRIHNADDGVEKKHLNSLSIVTLFLATYLMLKIVLEHIFTFQFPL 240

Query: 248 HVAAFVLLLMLLASPLYIAIRAQQRESTNILLSSLTESDQLIGHSNQAIMDFDNEWPRES 307
            VA+F+LLL+LLASPLY+AIRAQQRES  IL  S+TESDQLI  SNQ   +FDNE  RES
Sbjct: 241 QVASFILLLILLASPLYVAIRAQQRESRKILHPSVTESDQLISRSNQESEEFDNERRRES 300

Query: 308 EENLNLLQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLEISTLVSLWSIW 367
           EE+LNL QALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLE + LVSLWSIW
Sbjct: 301 EESLNLFQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLETTILVSLWSIW 360

Query: 368 NFFGRFGAGYVSDYFLHARGWARPLFMFLTLTTMSIGHVVIASGLPGALFAGSVIVGVCY 427
           NFFGR GAGYVSDYFLHA+GWARPLFMF+TL TMSIGHVVIASGLPGALFAGSV+VGVCY
Sbjct: 361 NFFGRLGAGYVSDYFLHAKGWARPLFMFITLATMSIGHVVIASGLPGALFAGSVLVGVCY 420

Query: 428 GSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYLFSVRVVGYIYDKEASGEGDTCTG 487
           GSQWSLMPTITSEIFGVVHMGTIFNAITIASP+GSY+FSVRV+GY+YDKEASGEG TCTG
Sbjct: 421 GSQWSLMPTITSEIFGVVHMGTIFNAITIASPVGSYIFSVRVIGYMYDKEASGEGGTCTG 480

Query: 488 TNCFMLSFLIMALATLLGSWATLGLFFWRRSFYDQVVFRRLQ 530
           T CFMLSFLIMA ATLLGS A LGLFFWRRSFYDQVVFRRLQ
Sbjct: 481 TYCFMLSFLIMAFATLLGSLAALGLFFWRRSFYDQVVFRRLQ 522

[Close]

BLAST of MS001965 vs. ExPASy TrEMBL
Match: A0A0A0LPA7 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G008450 PE=4 SV=1)

HSP 1 Score: 902.9 bits (2332), Expect = 6.3e-259
Identity = 460/525 (87.62%), Postives = 479/525 (91.24%), Query Frame = 0


Query: 5   ELEMETPRLNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQRYDQSTLDIVSVFKDIG 64
           ELE ET  L NKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQ YDQSTLDIVSVFKDIG
Sbjct: 2   ELESETSSLKNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQGYDQSTLDIVSVFKDIG 61

Query: 65  VNCGVLSGFLYYYAAADGGRRGPWIVHLAGAIQCFLGYFLIWAAVAGLFPRPPVPAMCLF 124
           VNCGVL+GFLYY+A A GGR GPWIVH AGAIQCFLGYF IWAAV G+ PRPPVP MCLF
Sbjct: 62  VNCGVLAGFLYYFATAHGGRPGPWIVHFAGAIQCFLGYFFIWAAVYGVLPRPPVPVMCLF 121

Query: 125 MLVAAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFDEQPTSF 184
           MLVAAHAQSFFNTANVVTGVRNFP YSGTIVGIMKGFLGLSGAILIQ YETIF+ QPTSF
Sbjct: 122 MLVAAHAQSFFNTANVVTGVRNFPRYSGTIVGIMKGFLGLSGAILIQTYETIFNGQPTSF 181

Query: 185 ILMLALLPTLNSLLFMWFVRIHNTNDGIEKEHLNTLSIITLLVAIYLMFKIVLEHIFTFQ 244
           +LMLALLPTLNSLL MWFVRIH+ +DGIEKEHLNTLSIITL+VA YLM KIVLEHIFTFQ
Sbjct: 182 LLMLALLPTLNSLLCMWFVRIHHVDDGIEKEHLNTLSIITLVVATYLMIKIVLEHIFTFQ 241

Query: 245 FPLHVAAFVLLLMLLASPLYIAIRAQQRESTNILLSSLTESDQLIGHSNQAIMDFDNEWP 304
           FPLHVA F+LLLMLLASPLYIAIRAQ RES  IL  S TESDQLIG  NQ   DFD+E  
Sbjct: 242 FPLHVATFILLLMLLASPLYIAIRAQPRESRRILHPSFTESDQLIGRHNQETSDFDHERG 301

Query: 305 RESEENLNLLQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLEISTLVSLW 364
           RESEE+L L QALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTS EI+TLVSLW
Sbjct: 302 RESEESLTLFQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSSEINTLVSLW 361

Query: 365 SIWNFFGRFGAGYVSDYFLHARGWARPLFMFLTLTTMSIGHVVIASGLPGALFAGSVIVG 424
           SIWNFFGRFGAGYVSDY+LHA+GWARPLFMF+TL TMSIGHVVIASGLPGALFAGS++VG
Sbjct: 362 SIWNFFGRFGAGYVSDYYLHAKGWARPLFMFITLMTMSIGHVVIASGLPGALFAGSIVVG 421

Query: 425 VCYGSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYLFSVRVVGYIYDKEASGEGDT 484
           VCYGSQWSLMPTITSEIFGVVHMGTIFNAIT+ASP+GSYLFSVRVVGYIYDKEAS EGDT
Sbjct: 422 VCYGSQWSLMPTITSEIFGVVHMGTIFNAITVASPVGSYLFSVRVVGYIYDKEASSEGDT 481

Query: 485 CTGTNCFMLSFLIMALATLLGSWATLGLFFWRRSFYDQVVFRRLQ 530
           C GT CFMLSF IMA ATLLGS A LGLFFWRRSFYDQVV RRLQ
Sbjct: 482 CIGTYCFMLSFFIMAFATLLGSLAALGLFFWRRSFYDQVVVRRLQ 526

[Close]

BLAST of MS001965 vs. ExPASy TrEMBL
Match: A0A6J1F4N1 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111440684 PE=4 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 2.4e-258
Identity = 457/522 (87.55%), Postives = 484/522 (92.72%), Query Frame = 0


Query: 8   METPRLNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQRYDQSTLDIVSVFKDIGVNC 67
           ME    NNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQ YDQSTLDIVSVFKDIGVNC
Sbjct: 1   MEISGFNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQGYDQSTLDIVSVFKDIGVNC 60

Query: 68  GVLSGFLYYYAAADGGRRGPWIVHLAGAIQCFLGYFLIWAAVAGLFPRPPVPAMCLFMLV 127
           GVL+GFLYYYA ADGG   PWIVHLAGAIQCFLGYFL+WAAVAG+FPRPP+PAMC FMLV
Sbjct: 61  GVLAGFLYYYATADGGGSRPWIVHLAGAIQCFLGYFLMWAAVAGVFPRPPLPAMCFFMLV 120

Query: 128 AAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFDEQPTSFILM 187
           AAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIF+EQPTSF+LM
Sbjct: 121 AAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFNEQPTSFLLM 180

Query: 188 LALLPTLNSLLFMWFVRIHNTNDGIEKEHLNTLSIITLLVAIYLMFKIVLEHIFTFQFPL 247
           LALLPTLNSLLFMWFVRIHN +D ++K+HLN+LSI+TL +A YLM KIVLEHIFTFQFPL
Sbjct: 181 LALLPTLNSLLFMWFVRIHNADDEVDKKHLNSLSIVTLFLATYLMLKIVLEHIFTFQFPL 240

Query: 248 HVAAFVLLLMLLASPLYIAIRAQQRESTNILLSSLTESDQLIGHSNQAIMDFDNEWPRES 307
            VA+F+LLL+LLASPLY+AIRAQQRES  IL  S+TESDQLI  SNQ   DFDNE  RES
Sbjct: 241 QVASFILLLILLASPLYVAIRAQQRESRKILHPSVTESDQLISRSNQESEDFDNERRRES 300

Query: 308 EENLNLLQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLEISTLVSLWSIW 367
           EE+LNL QALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLE + LVSLWSIW
Sbjct: 301 EESLNLFQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLETTILVSLWSIW 360

Query: 368 NFFGRFGAGYVSDYFLHARGWARPLFMFLTLTTMSIGHVVIASGLPGALFAGSVIVGVCY 427
           NFFGR GAGYVSDYFLHA+GWARPLFMF+TL TMSIGHVVIASGLPGALFAGSV+VGVCY
Sbjct: 361 NFFGRLGAGYVSDYFLHAKGWARPLFMFITLATMSIGHVVIASGLPGALFAGSVLVGVCY 420

Query: 428 GSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYLFSVRVVGYIYDKEASGEGDTCTG 487
           GSQWSLMPTITSEIFGVVHMGTIFNAITIASP+GSY+FSVRV+GY+YDKEASGEG TCTG
Sbjct: 421 GSQWSLMPTITSEIFGVVHMGTIFNAITIASPVGSYIFSVRVIGYMYDKEASGEGGTCTG 480

Query: 488 TNCFMLSFLIMALATLLGSWATLGLFFWRRSFYDQVVFRRLQ 530
           T CFMLSFLIMA ATLLGS A LGLFF RRSFYDQVVFRRLQ
Sbjct: 481 TYCFMLSFLIMAFATLLGSLAALGLFFRRRSFYDQVVFRRLQ 522

[Close]

BLAST of MS001965 vs. ExPASy TrEMBL
Match: A0A5A7UUS7 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G002250 PE=4 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 4.1e-258
Identity = 454/525 (86.48%), Postives = 482/525 (91.81%), Query Frame = 0


Query: 5   ELEMETPRLNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQRYDQSTLDIVSVFKDIG 64
           ELE +T  LNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQ YDQSTLDIVSVFKDIG
Sbjct: 2   ELESDTSGLNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQGYDQSTLDIVSVFKDIG 61

Query: 65  VNCGVLSGFLYYYAAADGGRRGPWIVHLAGAIQCFLGYFLIWAAVAGLFPRPPVPAMCLF 124
           VNCGVL+GFLYY+A   GGR GPWIVH AGAIQCFLGYF IWAAV G+F RPPVP MCLF
Sbjct: 62  VNCGVLAGFLYYFATTHGGRPGPWIVHFAGAIQCFLGYFFIWAAVYGVFHRPPVPVMCLF 121

Query: 125 MLVAAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFDEQPTSF 184
           MLVAAHAQSFFNTANVVTGVRNFP YSGTIVGIMKGFLGLSGAILIQ+YETIF+ QPTSF
Sbjct: 122 MLVAAHAQSFFNTANVVTGVRNFPRYSGTIVGIMKGFLGLSGAILIQIYETIFNGQPTSF 181

Query: 185 ILMLALLPTLNSLLFMWFVRIHNTNDGIEKEHLNTLSIITLLVAIYLMFKIVLEHIFTFQ 244
           +LMLALLPTLNSLL MWFVRIH+ +DGIEKEHLNTLSIITL++A YLM KIVLEHIFTFQ
Sbjct: 182 LLMLALLPTLNSLLCMWFVRIHHVDDGIEKEHLNTLSIITLVIATYLMIKIVLEHIFTFQ 241

Query: 245 FPLHVAAFVLLLMLLASPLYIAIRAQQRESTNILLSSLTESDQLIGHSNQAIMDFDNEWP 304
           FPLHVA F+LLLMLLASPLYIAIRAQQRES   L  S  ESDQLIG  N+  +DFD+E  
Sbjct: 242 FPLHVATFILLLMLLASPLYIAIRAQQRESRRTLHPSFAESDQLIGRHNRETLDFDHERG 301

Query: 305 RESEENLNLLQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLEISTLVSLW 364
           RESEE+L L+QALYTIDFWILFFATACGMGTGLATVNN+SQIGLSLGYTS EI+TLVSLW
Sbjct: 302 RESEESLTLIQALYTIDFWILFFATACGMGTGLATVNNMSQIGLSLGYTSSEINTLVSLW 361

Query: 365 SIWNFFGRFGAGYVSDYFLHARGWARPLFMFLTLTTMSIGHVVIASGLPGALFAGSVIVG 424
           SIWNFFGRFGAGYVSDY+LHA+GWARPLFMF+TLTTMSIGHVVIASGLPGALFAGS++VG
Sbjct: 362 SIWNFFGRFGAGYVSDYYLHAKGWARPLFMFITLTTMSIGHVVIASGLPGALFAGSIVVG 421

Query: 425 VCYGSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYLFSVRVVGYIYDKEASGEGDT 484
           VCYGSQWSLMPTITSEIFGV+HMGTIFNAIT+ASP+GSYLFSVRVVGYIYDKEAS EGD 
Sbjct: 422 VCYGSQWSLMPTITSEIFGVLHMGTIFNAITVASPVGSYLFSVRVVGYIYDKEASSEGDA 481

Query: 485 CTGTNCFMLSFLIMALATLLGSWATLGLFFWRRSFYDQVVFRRLQ 530
           C GT CFMLSF IMA ATLLGS+A LGLFFWRRSFYDQVV RRLQ
Sbjct: 482 CIGTYCFMLSFFIMAFATLLGSFAALGLFFWRRSFYDQVVIRRLQ 526

[Close]

BLAST of MS001965 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 586.3 bits (1510), Expect = 2.5e-167
Identity = 310/528 (58.71%), Postives = 380/528 (71.97%), Query Frame = 0


Query: 13  LNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQRYDQSTLDIVSVFKDIGVNCGVLSG 72
           L  KWV+  AS+WIQC SG+ YTF IYS  LKSTQ YDQSTLD VSVFKDIG N GV SG
Sbjct: 4   LRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVFSG 63

Query: 73  FLYYYAAAD---------GGRRGPWIVHLAGAIQCFLGYFLIWAAVAGLFPRPPVPAMCL 132
            LY YA ++         GG  GPW+V   GAIQCF GYFLIWA+V GL  +PPVP MCL
Sbjct: 64  LLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLMCL 123

Query: 133 FMLVAAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFDEQPTS 192
           FM +AA +Q+FFNTANVV+ V NF  Y GT VGIMKGFLGLSGAILIQ+YET+    P S
Sbjct: 124 FMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDPAS 183

Query: 193 FILMLALLPTLNSLLFMWFVRIHNTNDGIEKEHLNTLSIITLLVAIYLMFKIVLEHIFTF 252
           FIL+LA+ PT+ SLL M  VRI+ T+   +K+HLN LS ++L++A YLM  I+L++ F  
Sbjct: 184 FILLLAVTPTVLSLLVMPLVRIYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKNTFGL 243

Query: 253 QFPLHVAAFVLLLMLLASPLYIAIRAQQRESTNIL---LSSLTESDQLIGHSNQAIMDFD 312
               ++   V LL++LA PL IA RAQ+      +    S L  S +     NQ+  + D
Sbjct: 244 SSWANIVTLVCLLVMLALPLLIARRAQRDGMEKTVPHDYSPLISSPKATTSGNQS-SEGD 303

Query: 313 NEWPRESEENLNLLQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLEISTL 372
           ++      ENLNLLQA+  + FW+LF A  CGMG+GL+T+NNI QIG SL Y+S+EI++L
Sbjct: 304 SKVEAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQIGESLRYSSVEINSL 363

Query: 373 VSLWSIWNFFGRFGAGYVSDYFLHARGWARPLFMFLTLTTMSIGHVVIASGLPGALFAGS 432
           VSLWSIWNF GRFGAGY SD  LH +GW RPL M  TL TMSIGH++IASG  G L+ GS
Sbjct: 364 VSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHLIIASGFQGNLYVGS 423

Query: 433 VIVGVCYGSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYLFSVRVVGYIYDKEASG 492
           VIVGVCYGSQWSLMPTITSE+FG+ HMGTIFN I++ASPIGSY+FSVR++GYIYDK ASG
Sbjct: 424 VIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIFSVRLIGYIYDKTASG 483

Query: 493 EGDTCTGTNCFMLSFLIMALATLLGSWATLGLFFWRRSFYDQVVFRRL 529
           EG+TC G++CF LSF+IMA     G    + LFF  ++ Y Q++ +RL
Sbjct: 484 EGNTCYGSHCFRLSFIIMASVAFFGFLVAIVLFFRTKTLYRQILVKRL 530

[Close]

BLAST of MS001965 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 540.4 bits (1391), Expect = 1.6e-153
Identity = 283/525 (53.90%), Postives = 374/525 (71.24%), Query Frame = 0


Query: 8   METPRLNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQRYDQSTLDIVSVFKDIGVNC 67
           M T  L  KW++  AS+WIQC++G  YTF IYS  LKSTQ YDQSTLD VSVFKDIG N 
Sbjct: 1   MATEILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNV 60

Query: 68  GVLSGFLYYYAAADGGRR-------GPWIVHLAGAIQCFLGYFLIWAAVAGLFPRPPVPA 127
           GVLSG +Y  A  +  RR       GPW+V L GAI  F GYFL+WA+V GL  RPPVP 
Sbjct: 61  GVLSGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPV 120

Query: 128 MCLFMLVAAHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFDEQ 187
           MCLFM +AA + +F NTANVV+ + NF  Y GT VGIMKGF+GLSGA+LIQ+YE +    
Sbjct: 121 MCLFMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGD 180

Query: 188 PTSFILMLALLPTLNSLLFMWFVRIHNTNDGIEKEHLNTLSIITLLVAIYLMFKIVLEHI 247
           P +FIL+LA++P+L S+L M  VR++ T+   EK+HL+ LS ++L++A YLM  I+L+  
Sbjct: 181 PKTFILLLAIVPSLLSVLVMPLVRVYKTSTVDEKKHLDGLSTLSLIIAAYLMITIILKST 240

Query: 248 FTFQFPLHVAAFVLLLMLLASPLYIAIRAQQRESTNILLSSLTES--DQLIGHSNQAIMD 307
            +     +     +LL+LL+SPL +A+RA  R+S    LSS+     D L   ++  I+ 
Sbjct: 241 LSLPSWANAVTLAVLLVLLSSPLLVAVRA-HRDSIEKPLSSVYSPLVDNLEATTSGEILM 300

Query: 308 FDNEWPRESEENLNLLQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLEIS 367
            D       +++LNLLQA+  +DFW+LF A  CGMG+G++T+NNI QIG SL YTS+EI+
Sbjct: 301 LD------EDKSLNLLQAMCNVDFWLLFLAMICGMGSGISTINNIRQIGESLRYTSVEIN 360

Query: 368 TLVSLWSIWNFFGRFGAGYVSDYFLHARGWARPLFMFLTLTTMSIGHVVIASGLPGALFA 427
           +L++LW+IWNF GRFG GYVSD+ LH +GW RPL M  TL TM+IGH++IASG  G L+ 
Sbjct: 361 SLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLGTMTIGHLIIASGFQGNLYP 420

Query: 428 GSVIVGVCYGSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYLFSVRVVGYIYDKEA 487
           GS+IVG+CYGSQWSLMPTITSE+FGV HMGTI+N I+IASP+GSY+FSVR++GYIYD+  
Sbjct: 421 GSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIASPMGSYIFSVRLIGYIYDRTI 480

Query: 488 SGEGDTCTGTNCFMLSFLIMALATLLGSWATLGLFFWRRSFYDQV 524
            GEG+TC G +CF L+++++A    LG   +  L F  ++ Y Q+
Sbjct: 481 IGEGNTCYGPHCFRLAYVVIASVAFLGFLVSCVLVFRTKTIYRQI 518

[Close]

BLAST of MS001965 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 530.4 bits (1365), Expect = 1.6e-150
Identity = 274/521 (52.59%), Postives = 370/521 (71.02%), Query Frame = 0


Query: 12  RLNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQRYDQSTLDIVSVFKDIGVNCGVLS 71
           R+N KWV+  AS+WIQ  SG+ YTF+IYS  LKS+Q YDQSTLD VSVFKDIG   G++S
Sbjct: 3   RINTKWVAAAASIWIQSFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIIS 62

Query: 72  GFLY-YYAAADGGRRGPWIVHLAGAIQCFLGYFLIWAAVAGLFPRPPVPAMCLFMLVAAH 131
           GFLY    +   G  GPW+V   G +Q F+G+F IWA+V GL   PPVP MCLF+ +A H
Sbjct: 63  GFLYTAMTSKSRGFGGPWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAGH 122

Query: 132 AQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIF--DEQPTSFILML 191
           +  FFNTANVVT  RNF  Y GT VGIM+GFLGLSGAILIQ+Y  +   +  P +FIL+L
Sbjct: 123 SLPFFNTANVVTAARNFSQYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFILLL 182

Query: 192 ALLPTLNSLLFMWFVRIHNTNDGIEKEHLNTLSIITLLVAIYLMFKIVLEHIFTFQFPLH 251
           A++PTL   L M FVR++ T    +K+HL+ LS I++++A YLM  I +E++      + 
Sbjct: 183 AIVPTLVMFLAMPFVRVYETVTISDKKHLDGLSAISMIIAAYLMVVITVENVLGLSRSMQ 242

Query: 252 VAAFVLLLMLLASPLYIAIRA--QQRESTNILLSSLTESDQLIGHSNQAIMDFDNEWPRE 311
           + +F+L+L+LLASPL +A+RA  ++R++ + L   + ++  L+   +  I     +    
Sbjct: 243 IFSFILVLLLLASPLLVAVRALREKRQTLSSLDGPVLDTSALLDPPSSNIFP---DGDHL 302

Query: 312 SEENLNLLQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLEISTLVSLWSI 371
             E+ N+L+A+ T++FW+LF A  CGMG+G ATVNN+ QIG SL Y+S+++++LVSLWSI
Sbjct: 303 VAEDSNILEAMSTVNFWLLFLAMLCGMGSGFATVNNMRQIGESLRYSSVQLNSLVSLWSI 362

Query: 372 WNFFGRFGAGYVSDYFLHARGWARPLFMFLTLTTMSIGHVVIASGLPGALFAGSVIVGVC 431
           WNF GRFGAGYVSD FLH   W RP+FM +TL  M+IGH+++ASG+ G+L+AGSV++G+ 
Sbjct: 363 WNFLGRFGAGYVSDTFLHKHSWPRPIFMAITLGVMAIGHIIVASGVQGSLYAGSVLIGMA 422

Query: 432 YGSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYLFSVRVVGYIYDKEASGEGDTCT 491
           YGSQWSLMPTITSEIFG+ HMGTI+  I+IA PIGSY+ SV+V+GY YDK AS + ++C 
Sbjct: 423 YGSQWSLMPTITSEIFGIRHMGTIYFTISIAGPIGSYILSVKVIGYFYDKVASEDDNSCF 482

Query: 492 GTNCFMLSFLIMALATLLGSWATLGLFFWRRSFYDQVVFRR 528
           G+ CF  SF+IMA   L GS     LFF    FY  +V +R
Sbjct: 483 GSQCFRTSFMIMASVALFGSLVASVLFFRTHKFYKNLVAKR 520

[Close]

BLAST of MS001965 vs. TAIR 10
Match: AT2G34350.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 530.4 bits (1365), Expect = 1.6e-150
Identity = 277/522 (53.07%), Postives = 366/522 (70.11%), Query Frame = 0


Query: 14  NNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQRYDQSTLDIVSVFKDIGVNCGVLSGF 73
           N KWV+  AS+WIQ  SG+ YTF IYS  LKS+Q YDQSTLD VSV+KDIG N G+LSG 
Sbjct: 5   NTKWVAAAASIWIQSFSGASYTFGIYSSVLKSSQSYDQSTLDTVSVYKDIGANVGILSGL 64

Query: 74  LYYYAAA-----DGGRRGPWIVHLAGAIQCFLGYFLIWAAVAGLFPRPPVPAMCLFMLVA 133
            Y   A+      G   GPW+V   G +Q F+GY  IW A +G+ PRPPV  MCLFM  A
Sbjct: 65  FYTAVASRKSGNGGFFSGPWLVIFVGLLQWFVGYGFIWMATSGVIPRPPVAMMCLFMFFA 124

Query: 134 AHAQSFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFDEQPTSFILML 193
            H Q FFNTA VVT VRNF  Y GT VGIMKG+LGLSGAIL+Q+Y       P ++IL+L
Sbjct: 125 GHCQPFFNTAIVVTAVRNFSDYGGTAVGIMKGYLGLSGAILVQMYHIFCGGDPRNYILLL 184

Query: 194 ALLPTLNSLLFMWFVRIHNTNDGIEKEHLNTLSIITLLVAIYLMFKIVLEHIFTFQFPLH 253
           A++P+L  L  M FVR ++T    +K+HLN LS I+L++  YLM  I++E+I     P+ 
Sbjct: 185 AVVPSLLILTLMPFVRTYDTVIAGDKKHLNGLSAISLIIVTYLMVVILVENIIGMSMPMK 244

Query: 254 VAAFVLLLMLLASPLYIAIRAQQRESTNILLSS--LTESDQLIGHSNQAIMDFDNEWPRE 313
           + +F  LL+LLASPL +A+RAQ+ E    L     +TE   L+   +   ++  ++    
Sbjct: 245 ICSFTFLLLLLASPLLVAVRAQREEEHRFLSLDFPVTERTTLL---DSPKLNSSSDVKDV 304

Query: 314 SEENLNLLQALYTIDFWILFFATACGMGTGLATVNNISQIGLSLGYTSLEISTLVSLWSI 373
              ++N+L+A+ T +FW+LF A  CGMG+GLAT+NNI Q+G SL Y+++++++LVSLWSI
Sbjct: 305 MTNDMNVLEAICTTNFWLLFVAMICGMGSGLATINNIRQMGESLRYSTVQLNSLVSLWSI 364

Query: 374 WNFFGRFGAGYVSDYFLHARGWARPLFMFLTLTTMSIGHVVIASGLPGALFAGSVIVGVC 433
           WNF GRFG+GY+SD +LH+ GW RP+FM +TL  M+IGH+V+ASGL G+L+ GS++VG+ 
Sbjct: 365 WNFLGRFGSGYISDTYLHSHGWPRPVFMAITLGLMAIGHIVMASGLLGSLYIGSLLVGLA 424

Query: 434 YGSQWSLMPTITSEIFGVVHMGTIFNAITIASPIGSYLFSVRVVGYIYDKEASGEGDTCT 493
           YGSQWSLMPTITSEIFGV+HMGTIF  I+IASP+GSY FSV+V+GY+YDK AS +  +C 
Sbjct: 425 YGSQWSLMPTITSEIFGVLHMGTIFYTISIASPVGSYFFSVKVIGYLYDKVASEDDHSCY 484

Query: 494 GTNCFMLSFLIMALATLLGSWATLGLFFWRRSFYDQVVFRRL 529
           G +CF  SFLIMA   LLGS   L L    + FY  +V +R+
Sbjct: 485 GNHCFRTSFLIMAAMALLGSLVALVLLLRTKKFYATLVAKRI 523

[Close]

BLAST of MS001965 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 336.3 bits (861), Expect = 4.6e-92
Identity = 208/562 (37.01%), Postives = 310/562 (55.16%), Query Frame = 0


Query: 13  LNNKWVSTVASVWIQCTSGSLYTFSIYSQTLKSTQRYDQSTLDIVSVFKDIGVNCGVLSG 72
           L  +W     S+ I  T+G+ Y F IYS  +K T  YDQ+TL+++S FKD+G N GVL+G
Sbjct: 18  LTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVLAG 77

Query: 73  FLYYYAAADGGRRGPWIVHLAGAIQCFLGYFLIWAAVAGLFPRPPVPAMCLFMLVAAHAQ 132
            L            PW + L GAI  F GYF+IW AV     +P V  MCL++ V A++Q
Sbjct: 78  LLNEVTP-------PWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQ 137

Query: 133 SFFNTANVVTGVRNFPSYSGTIVGIMKGFLGLSGAILIQVYETIFDEQPTSFILMLALLP 192
           SF NT ++VT V+NFP   G ++GI+KG++GLSGAI+ Q+Y   + E     ILM+  LP
Sbjct: 138 SFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWLP 197

Query: 193 TLNSLLFMWFVRIHNTNDGIEKEHLNTLSI------ITLLVAIYLMFKIVLEHIFTFQFP 252
            + S  F+  +RI        K   N L +      I+L +A +LM  I++  +  F   
Sbjct: 198 AIVSFAFLRTIRIMKV-----KRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQS 257

Query: 253 LHVAAFVLLLMLLASPLYIAIRAQQR--ESTNILLSS------LTESDQLIGHSNQAIMD 312
               +  ++++LL  P+ + I  +++  +   + L+       +TE  +L    +    D
Sbjct: 258 EFGGSAAVVIVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKL---DSSEFKD 317

Query: 313 FDNE--------------W-----PRESEENLNLLQALYTIDFWILFFATACGMGTGLAT 372
            D E              W     P E  ++  +LQAL+++D  ILF AT CG+G  L  
Sbjct: 318 DDGEESKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTA 377

Query: 373 VNNISQIGLSLGYTSLEISTLVSLWSIWNFFGRFGAGYVSDYFLHARGWARPLFMFLTLT 432
           ++N+ QIG SLGY    +ST VSL SIWN++GR  +G VS+ FL    + RPL + + L 
Sbjct: 378 IDNLGQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLL 437

Query: 433 TMSIGHVVIASGLPGALFAGSVIVGVCYGSQWSLMPTITSEIFGVVHMGTIFNAITIASP 492
               GH++IA  +PG L+  SVI+G C+G+QW L+  I SEIFG+ +  T++N  ++ASP
Sbjct: 438 LSCAGHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASP 497

Query: 493 IGSYLFSVRVVGYIYDKEA------------SGEGDTCTGTNCFMLSFLIMALATLLGSW 530
           IGSYL +VRV GY+YD EA             G+   C GT+CF LSF+I+A  TL G  
Sbjct: 498 IGSYLLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVL 557

The following BLAST results are available for this feature:

BLAST of MS001965 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135043.1	1.6e-293	99.23	protein NUCLEAR FUSION DEFECTIVE 4-like [Momordica charantia] >XP_022135044.1 pr...	[more]
XP_038879767.1	3.9e-263	89.71	protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida]	[more]
XP_023530012.1	8.2e-261	88.12	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo] >XP_0235300...	[more]
XP_022972552.1	4.1e-260	87.74	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022972555.1 prote...	[more]
XP_004138144.1	1.3e-258	87.62	protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >XP_031740770.1 protein NUC...	[more]

BLAST of MS001965 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I9E1	7.5e-31	25.28	Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ...	[more]
Q6CGU8	2.9e-06	24.43	Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=28...	[more]
Q07376	4.6e-04	21.86	Probable transporter MCH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288...	[more]
Q6FWD4	7.8e-04	23.11	Probable transporter MCH1 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM ...	[more]

BLAST of MS001965 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C1J4	7.9e-294	99.23	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1I6E1	2.0e-260	87.74	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111471...	[more]
A0A0A0LPA7	6.3e-259	87.62	Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G00845...	[more]
A0A6J1F4N1	2.4e-258	87.55	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A5A7UUS7	4.1e-258	86.48	Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]

BLAST of MS001965 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G74780.1	2.5e-167	58.71	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT1G18940.1	1.6e-153	53.90	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT2G34355.1	1.6e-150	52.59	Major facilitator superfamily protein	[more]
AT2G34350.1	1.6e-150	53.07	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT2G39210.1	4.6e-92	37.01	Major facilitator superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 217..509 e-value: 2.1E-8 score: 35.3
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 222..516
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 24..206
IPR010658	Nodulin-like	PFAM	PF06813	Nodulin-like	coord: 16..267 e-value: 1.1E-64 score: 218.3
None	No IPR available	PANTHER	PTHR21576	UNCHARACTERIZED NODULIN-LIKE PROTEIN	coord: 12..529
None	No IPR available	PANTHER	PTHR21576:SF133	PROTEIN NUCLEAR FUSION DEFECTIVE 4-LIKE	coord: 12..529
None	No IPR available	CDD	cd17354	MFS_Mch1p_like	coord: 16..505 e-value: 1.28644E-119 score: 355.406

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS001965.1	MS001965.1	mRNA