MS001945 (gene) Bitter gourd (TR) v1

Overview
NameMS001945
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationscaffold30: 1197911 .. 1198786 (+)
RNA-Seq ExpressionMS001945
SyntenyMS001945
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCATCGATCTCCGAAGAAGAGGAGGAATTATTGTCGAAGCTCAAGAGTCCTGAAGTGTTTAAGCAAGAAGAAGGTGTTATTTCGCTGAGAAAGATTACGAAAGCCGACGAGAAAATTAGGGTTTCGCTTTGCACGCCTCGGATTCTTTTTGCCCTCCGCCTCTTGATAACATCGAGATACTCCACCGTACAAATCAACGCCGTCGCCTCTCTGGTGAATCTCTCCCTCGAAAGGCGCAACAAAGTGAAGATCGTGCGGTCAGGATTGGTTCCGAACTTAATCGACGTGCTAAAAGGGGGACATACCGAGTCGCAAGAACACGCCGCCGGCGCGCTCTTCAGCCTGGCGTTGGAGGACGAAAATCGGATGGCGATCGGAGTCCTCGGCGCGCTTCAGCCGCTCCTCTACGCGCTCCGATCGGAAAGCGAGCGGACTCGAGACGATTCCGCTCTGTGTCTGTACAACCTAACAATGATCCAGAGCAACCGAGTGAAGCTCGTGAAACTCGGCGCCGCGACAACTCTACTTTCCATGGTGAAATCGGGAAACTCGGTGGACCGGCTGCTGCTGATTCTCTGCAACATCGCCGTGTGCCCGGAGGGGAGGTCGGCAATGCTGGACGCCGACGCCGTCGGGTGCTTGGTGGAGATGCTGAGGACGAAGGAGGTGAACTCCGAGTCGACTCGCGAGAACTGCGTGGCGGCATTGTACGCGCTCAGCTTCGGAAGTATGAGATTCAGAGGACTGGCGAAAGAAGCTAGGGCAATGGAGGTGCTGCGAGAGATTGTGGACGGAGGAAGCGAGAGGGCGAGGGAGAAGGCGAAGATGATTTTGCAGAGGATGAAGGGAAGATTCGACGGAGACGATGAAGAAGAA

mRNA sequence

TCATCGATCTCCGAAGAAGAGGAGGAATTATTGTCGAAGCTCAAGAGTCCTGAAGTGTTTAAGCAAGAAGAAGGTGTTATTTCGCTGAGAAAGATTACGAAAGCCGACGAGAAAATTAGGGTTTCGCTTTGCACGCCTCGGATTCTTTTTGCCCTCCGCCTCTTGATAACATCGAGATACTCCACCGTACAAATCAACGCCGTCGCCTCTCTGGTGAATCTCTCCCTCGAAAGGCGCAACAAAGTGAAGATCGTGCGGTCAGGATTGGTTCCGAACTTAATCGACGTGCTAAAAGGGGGACATACCGAGTCGCAAGAACACGCCGCCGGCGCGCTCTTCAGCCTGGCGTTGGAGGACGAAAATCGGATGGCGATCGGAGTCCTCGGCGCGCTTCAGCCGCTCCTCTACGCGCTCCGATCGGAAAGCGAGCGGACTCGAGACGATTCCGCTCTGTGTCTGTACAACCTAACAATGATCCAGAGCAACCGAGTGAAGCTCGTGAAACTCGGCGCCGCGACAACTCTACTTTCCATGGTGAAATCGGGAAACTCGGTGGACCGGCTGCTGCTGATTCTCTGCAACATCGCCGTGTGCCCGGAGGGGAGGTCGGCAATGCTGGACGCCGACGCCGTCGGGTGCTTGGTGGAGATGCTGAGGACGAAGGAGGTGAACTCCGAGTCGACTCGCGAGAACTGCGTGGCGGCATTGTACGCGCTCAGCTTCGGAAGTATGAGATTCAGAGGACTGGCGAAAGAAGCTAGGGCAATGGAGGTGCTGCGAGAGATTGTGGACGGAGGAAGCGAGAGGGCGAGGGAGAAGGCGAAGATGATTTTGCAGAGGATGAAGGGAAGATTCGACGGAGACGATGAAGAAGAA

Coding sequence (CDS)

TCATCGATCTCCGAAGAAGAGGAGGAATTATTGTCGAAGCTCAAGAGTCCTGAAGTGTTTAAGCAAGAAGAAGGTGTTATTTCGCTGAGAAAGATTACGAAAGCCGACGAGAAAATTAGGGTTTCGCTTTGCACGCCTCGGATTCTTTTTGCCCTCCGCCTCTTGATAACATCGAGATACTCCACCGTACAAATCAACGCCGTCGCCTCTCTGGTGAATCTCTCCCTCGAAAGGCGCAACAAAGTGAAGATCGTGCGGTCAGGATTGGTTCCGAACTTAATCGACGTGCTAAAAGGGGGACATACCGAGTCGCAAGAACACGCCGCCGGCGCGCTCTTCAGCCTGGCGTTGGAGGACGAAAATCGGATGGCGATCGGAGTCCTCGGCGCGCTTCAGCCGCTCCTCTACGCGCTCCGATCGGAAAGCGAGCGGACTCGAGACGATTCCGCTCTGTGTCTGTACAACCTAACAATGATCCAGAGCAACCGAGTGAAGCTCGTGAAACTCGGCGCCGCGACAACTCTACTTTCCATGGTGAAATCGGGAAACTCGGTGGACCGGCTGCTGCTGATTCTCTGCAACATCGCCGTGTGCCCGGAGGGGAGGTCGGCAATGCTGGACGCCGACGCCGTCGGGTGCTTGGTGGAGATGCTGAGGACGAAGGAGGTGAACTCCGAGTCGACTCGCGAGAACTGCGTGGCGGCATTGTACGCGCTCAGCTTCGGAAGTATGAGATTCAGAGGACTGGCGAAAGAAGCTAGGGCAATGGAGGTGCTGCGAGAGATTGTGGACGGAGGAAGCGAGAGGGCGAGGGAGAAGGCGAAGATGATTTTGCAGAGGATGAAGGGAAGATTCGACGGAGACGATGAAGAAGAA

Protein sequence

SSISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRYSTVQINAVASLVNLSLERRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDENRMAIGVLGALQPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMVKSGNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTKEVNSESTRENCVAALYALSFGSMRFRGLAKEARAMEVLREIVDGGSERAREKAKMILQRMKGRFDGDDEEE
Homology
BLAST of MS001945 vs. NCBI nr
Match: XP_022135520.1 (U-box domain-containing protein 38 [Momordica charantia])

HSP 1 Score: 537.3 bits (1383), Expect = 8.0e-149
Identity = 291/292 (99.66%), Postives = 292/292 (100.00%), Query Frame = 0

Query: 1   SSISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRY 60
           SSISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRY
Sbjct: 214 SSISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRY 273

Query: 61  STVQINAVASLVNLSLERRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDE 120
           STVQINAVASLVNLSLE+RNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDE
Sbjct: 274 STVQINAVASLVNLSLEKRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDE 333

Query: 121 NRMAIGVLGALQPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMVK 180
           NRMAIGVLGALQPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMVK
Sbjct: 334 NRMAIGVLGALQPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMVK 393

Query: 181 SGNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTKEVNSESTRENCVAALYALS 240
           SGNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTKEVNSESTRENCVAALYALS
Sbjct: 394 SGNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTKEVNSESTRENCVAALYALS 453

Query: 241 FGSMRFRGLAKEARAMEVLREIVDGGSERAREKAKMILQRMKGRFDGDDEEE 293
           FGSMRFRGLAKEARAMEVLREIVDGGSERAREKAKMILQRMKGRFDGDDEEE
Sbjct: 454 FGSMRFRGLAKEARAMEVLREIVDGGSERAREKAKMILQRMKGRFDGDDEEE 505

BLAST of MS001945 vs. NCBI nr
Match: XP_038879975.1 (U-box domain-containing protein 38-like [Benincasa hispida])

HSP 1 Score: 456.8 bits (1174), Expect = 1.4e-124
Identity = 252/294 (85.71%), Postives = 269/294 (91.50%), Query Frame = 0

Query: 1   SSISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRY 60
           SSISEEE+  L KL+SP+VF+QEEGVISLRKITK DE IRVSLCTPRILF+L  LITSRY
Sbjct: 212 SSISEEEKNFLWKLESPDVFQQEEGVISLRKITKTDESIRVSLCTPRILFSLHRLITSRY 271

Query: 61  STVQINAVASLVNLSLERRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDE 120
             VQINAVASLVNLSLE+ NK+KI RSGLVP+LIDVLKGGHTE+QEHAAGALFSLALEDE
Sbjct: 272 PKVQINAVASLVNLSLEKPNKLKIARSGLVPHLIDVLKGGHTEAQEHAAGALFSLALEDE 331

Query: 121 NRMAIGVLGALQPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMVK 180
           NRMAIGVLGAL PLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGA TTLLSMVK
Sbjct: 332 NRMAIGVLGALPPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAVTTLLSMVK 391

Query: 181 SGNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTKEVNSESTRENCVAALYALS 240
           S NS +RLLLILCNIAVC EGRSAMLDADAVG LV MLR KE+NSESTRENCVAALYALS
Sbjct: 392 SRNSTNRLLLILCNIAVCQEGRSAMLDADAVGLLVGMLREKELNSESTRENCVAALYALS 451

Query: 241 FGSMRFRGLAKEARAMEVLREIVDGGSERAREKAKMILQRM--KGRFDGDDEEE 293
           +GSMRF+GLAKEA AMEVLREIV+ GSERAREKAK IL+RM  +GRFD DD+EE
Sbjct: 452 YGSMRFKGLAKEAGAMEVLREIVERGSERAREKAKKILERMRTRGRFDEDDDEE 505

BLAST of MS001945 vs. NCBI nr
Match: KAA0057894.1 (U-box domain-containing protein 38 [Cucumis melo var. makuwa])

HSP 1 Score: 446.0 bits (1146), Expect = 2.4e-121
Identity = 244/294 (82.99%), Postives = 269/294 (91.50%), Query Frame = 0

Query: 1   SSISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRY 60
           SSISE+E+  L+KL+SP+VF+QEEGV+SLRKITKADE IRVSLCTPRILF+L  LI SRY
Sbjct: 209 SSISEDEKNFLTKLESPDVFQQEEGVVSLRKITKADENIRVSLCTPRILFSLHRLIKSRY 268

Query: 61  STVQINAVASLVNLSLERRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDE 120
             VQINAVASLVNLSLE+ NK+KI RSGLVP+LIDVLKGGH+E+QEHAAGALFSLALEDE
Sbjct: 269 PKVQINAVASLVNLSLEKPNKLKIARSGLVPDLIDVLKGGHSEAQEHAAGALFSLALEDE 328

Query: 121 NRMAIGVLGALQPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMVK 180
           NRMAIGVLGAL PLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGA TTLLSMVK
Sbjct: 329 NRMAIGVLGALPPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAVTTLLSMVK 388

Query: 181 SGNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTKEVNSESTRENCVAALYALS 240
           S NS +RLLLILCN+AVC EGRSAMLDA+AV  LV MLR KE+NSESTRENCVAALYALS
Sbjct: 389 SRNSTNRLLLILCNMAVCQEGRSAMLDANAVELLVGMLREKELNSESTRENCVAALYALS 448

Query: 241 FGSMRFRGLAKEARAMEVLREIVDGGSERAREKAKMILQRM--KGRFDGDDEEE 293
           +GSMRF+GLAKEA AMEVLREIV+ GSERAREKAK IL+RM  +GRFD DD+++
Sbjct: 449 YGSMRFKGLAKEAGAMEVLREIVERGSERAREKAKKILERMRTRGRFDEDDDDD 502

BLAST of MS001945 vs. NCBI nr
Match: TYJ98582.1 (U-box domain-containing protein 38 [Cucumis melo var. makuwa])

HSP 1 Score: 444.1 bits (1141), Expect = 9.2e-121
Identity = 243/294 (82.65%), Postives = 268/294 (91.16%), Query Frame = 0

Query: 1   SSISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRY 60
           SSISE+E+  L+KL+SP+VF+QEEGV+SLRKITKADE IRVSLCTPRILF+L  LI SRY
Sbjct: 209 SSISEDEKNFLTKLESPDVFQQEEGVVSLRKITKADENIRVSLCTPRILFSLHRLIKSRY 268

Query: 61  STVQINAVASLVNLSLERRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDE 120
             VQINAVASLVNLSLE+ NK+KI RSGLVP+LIDVLKGGH+E+QEHAAGALFSLALEDE
Sbjct: 269 PKVQINAVASLVNLSLEKPNKLKIARSGLVPDLIDVLKGGHSEAQEHAAGALFSLALEDE 328

Query: 121 NRMAIGVLGALQPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMVK 180
           NRMAIGVLGAL PLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGA T LLSMVK
Sbjct: 329 NRMAIGVLGALPPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAVTNLLSMVK 388

Query: 181 SGNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTKEVNSESTRENCVAALYALS 240
           S NS +RLLLILCN+AVC EGRSAMLDA+AV  LV MLR KE+NSESTRENCVAALYALS
Sbjct: 389 SRNSTNRLLLILCNMAVCQEGRSAMLDANAVELLVGMLREKELNSESTRENCVAALYALS 448

Query: 241 FGSMRFRGLAKEARAMEVLREIVDGGSERAREKAKMILQRM--KGRFDGDDEEE 293
           +GSMRF+GLAKEA AMEVLREIV+ GSERAREKAK IL+RM  +GRFD DD+++
Sbjct: 449 YGSMRFKGLAKEAGAMEVLREIVERGSERAREKAKKILERMRTRGRFDEDDDDD 502

BLAST of MS001945 vs. NCBI nr
Match: XP_008453132.1 (PREDICTED: U-box domain-containing protein 38 [Cucumis melo])

HSP 1 Score: 444.1 bits (1141), Expect = 9.2e-121
Identity = 243/294 (82.65%), Postives = 268/294 (91.16%), Query Frame = 0

Query: 1   SSISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRY 60
           SSISE+E+  L+KL+SP+VF+QEEGV+SLRKITKADE IRVSLCTPRILF+L  LI SRY
Sbjct: 209 SSISEDEKNFLTKLESPDVFQQEEGVVSLRKITKADENIRVSLCTPRILFSLHRLIKSRY 268

Query: 61  STVQINAVASLVNLSLERRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDE 120
             VQINAVASLVNLSLE+ NK+KI RSGLVP+LIDVLKGGH+E+QEHAAGALFSLALEDE
Sbjct: 269 PKVQINAVASLVNLSLEKPNKLKIARSGLVPDLIDVLKGGHSEAQEHAAGALFSLALEDE 328

Query: 121 NRMAIGVLGALQPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMVK 180
           NRMAIGVLGAL PLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGA T LLSMVK
Sbjct: 329 NRMAIGVLGALPPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAVTNLLSMVK 388

Query: 181 SGNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTKEVNSESTRENCVAALYALS 240
           S NS +RLLLILCN+AVC EGRSAMLDA+AV  LV MLR KE+NSESTRENCVAALYALS
Sbjct: 389 SRNSTNRLLLILCNMAVCQEGRSAMLDANAVELLVGMLREKELNSESTRENCVAALYALS 448

Query: 241 FGSMRFRGLAKEARAMEVLREIVDGGSERAREKAKMILQRM--KGRFDGDDEEE 293
           +GSMRF+GLAKEA AMEVLREIV+ GSERAREKAK IL+RM  +GRFD DD+++
Sbjct: 449 YGSMRFKGLAKEAGAMEVLREIVERGSERAREKAKKILERMRTRGRFDEDDDDD 502

BLAST of MS001945 vs. ExPASy Swiss-Prot
Match: Q9FJP6 (U-box domain-containing protein 38 OS=Arabidopsis thaliana OX=3702 GN=PUB38 PE=1 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.0e-90
Identity = 186/300 (62.00%), Postives = 237/300 (79.00%), Query Frame = 0

Query: 1   SSISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRY 60
           S+ +EE+E + +KLKS E+F QE+G+I +RK+T+ +++ RVSLC+PRIL  L+ +I SRY
Sbjct: 220 STATEEDEVIYNKLKSSEIFDQEQGLIMMRKMTRTNDEARVSLCSPRILSLLKNMIVSRY 279

Query: 61  STVQINAVASLVNLSLERRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDE 120
           S VQ NA+ASLVNLSL+++NK+ IVR G VP LIDVLK G  E+QEHAAG +FSL+LED+
Sbjct: 280 SLVQTNALASLVNLSLDKKNKLTIVRLGFVPILIDVLKSGSREAQEHAAGTIFSLSLEDD 339

Query: 121 NRMAIGVLGALQPLLYALR-SESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMV 180
           N+M IGVLGALQPLL+ALR +ES+RTR DSAL LY+LT+ Q+NR KLV+LGA   L SMV
Sbjct: 340 NKMPIGVLGALQPLLHALRAAESDRTRHDSALALYHLTLNQTNRSKLVRLGAVPALFSMV 399

Query: 181 KSGNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLR-------TKEVNSESTRENC 240
           +SG S  R LL++CN+A C EGRSAMLDA+AV  LV  LR       T+  +S S RENC
Sbjct: 400 RSGESASRALLVICNLACCSEGRSAMLDANAVAILVGKLREEWTEEPTEARSSSSARENC 459

Query: 241 VAALYALSFGSMRFRGLAKEARAMEVLREIVDGGSERAREKAKMILQRMKGRFDGDDEEE 293
           VAAL+ALS  S+RF+GLAKEARA+EVL+E+ + G+ERAREKAK ILQ M+ R   DDEE+
Sbjct: 460 VAALFALSHESLRFKGLAKEARAVEVLKEVEERGTERAREKAKKILQLMRERVPEDDEED 519

BLAST of MS001945 vs. ExPASy Swiss-Prot
Match: Q0WUF6 (U-box domain-containing protein 41 OS=Arabidopsis thaliana OX=3702 GN=PUB41 PE=2 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 1.2e-86
Identity = 180/294 (61.22%), Postives = 226/294 (76.87%), Query Frame = 0

Query: 1   SSISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRY 60
           S +S EEEE+ +KL+  ++F  E+G+I LRK+T++ E +RVSLCT RIL  LR L+ SRY
Sbjct: 230 SPMSPEEEEIFNKLRGTDIFDHEQGLILLRKMTRSSEDLRVSLCTDRILSFLRSLLVSRY 289

Query: 61  STVQINAVASLVNLSLERRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDE 120
           + VQ NA AS+VNLSLE++NKVKIVRSG VP LIDVLK G TE+QEH AGALFSLALEDE
Sbjct: 290 NLVQTNAAASVVNLSLEKQNKVKIVRSGFVPLLIDVLKSGTTEAQEHVAGALFSLALEDE 349

Query: 121 NRMAIGVLGALQPLLYALR-SESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMV 180
           N+M IGVLGA++PLL+ALR SESER R D+AL LY+L++I SNR +LV+ GA  TLLSMV
Sbjct: 350 NKMVIGVLGAVEPLLHALRSSESERARQDAALALYHLSLIPSNRTRLVRAGAVPTLLSMV 409

Query: 181 KSGNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTKEV---NSESTRENCVAAL 240
           +SG+S  R+LL+LCN+A CP+G+ AMLD +AV  LV  LR  EV   +SE+ RENCVA L
Sbjct: 410 RSGDSTSRILLVLCNLAACPDGKGAMLDGNAVAILVGKLR--EVGGGDSEAARENCVAVL 469

Query: 241 YALSFGSMRFRGLAKEARAMEVLREIVDGGSERAREKAKMILQRMKGRFDGDDE 291
             L  G++RFRGLA EA A EVL E+ + G+ER +EKA  IL  M+G   G+ E
Sbjct: 470 LTLCQGNLRFRGLASEAGAEEVLMEVEENGNERVKEKASKILLAMRGGGGGESE 521

BLAST of MS001945 vs. ExPASy Swiss-Prot
Match: Q9FL17 (U-box domain-containing protein 40 OS=Arabidopsis thaliana OX=3702 GN=PUB40 PE=2 SV=2)

HSP 1 Score: 300.4 bits (768), Expect = 2.2e-80
Identity = 163/289 (56.40%), Postives = 216/289 (74.74%), Query Frame = 0

Query: 2   SISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRYS 61
           +++ EEE LL+KLKS  + + EE +IS+R+IT+ DE  R+SLCT R++ AL+ LI SRY+
Sbjct: 225 NLTPEEEALLTKLKSNRISEIEEALISIRRITRIDESSRISLCTTRVISALKSLIVSRYA 284

Query: 62  TVQINAVASLVNLSLERRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDEN 121
           TVQ+N  A LVNLSLE+ NKVKIVRSG+VP LIDVLK G  E+QEH+AG +FSLALEDEN
Sbjct: 285 TVQVNVTAVLVNLSLEKSNKVKIVRSGIVPPLIDVLKCGSVEAQEHSAGVIFSLALEDEN 344

Query: 122 RMAIGVLGALQPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMVKS 181
           + AIGVLG L+PLL+ +R  +E TR DSAL LY+L+++QSNR KLVKLGA   LL MV  
Sbjct: 345 KTAIGVLGGLEPLLHLIRVGTELTRHDSALALYHLSLVQSNRGKLVKLGAVQMLLGMVSL 404

Query: 182 GNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTKEVNSESTRENCVAALYALSF 241
           G  + R+LLILCN+A CP  R A+LD+  V C+V +LR     +ESTRE+CVA LY LS 
Sbjct: 405 GQMIGRVLLILCNMASCPVSRPALLDSGGVECMVGVLRRDREVNESTRESCVAVLYGLSH 464

Query: 242 -GSMRFRGLAKEARAMEVLREIVDGGSERAREKAKMILQRMKGRFDGDD 290
            G +RF+GLA  A A+E L ++   G ERA++KA+ +L+ ++ + + DD
Sbjct: 465 DGGLRFKGLAMAANAVEELVKVERSGRERAKQKARRVLEVLRAKIEDDD 513

BLAST of MS001945 vs. ExPASy Swiss-Prot
Match: Q9STT1 (U-box domain-containing protein 39 OS=Arabidopsis thaliana OX=3702 GN=PUB39 PE=1 SV=2)

HSP 1 Score: 299.3 bits (765), Expect = 4.8e-80
Identity = 169/286 (59.09%), Postives = 212/286 (74.13%), Query Frame = 0

Query: 3   ISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRYST 62
           +S EEEE+ +KL S +    E+G+I LRK T+++E  R+SLCT RIL  LR LI SRY+ 
Sbjct: 203 MSPEEEEIYNKLTSVDTIDHEQGLIQLRKTTRSNETTRISLCTDRILSLLRSLIVSRYNI 262

Query: 63  VQINAVASLVNLSLERRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDENR 122
           VQ NA AS+VNLSLE+ NK+KIVRSG VP LIDVLK G TE+QEH  GALFSLA+E+EN+
Sbjct: 263 VQTNAAASIVNLSLEKPNKLKIVRSGFVPLLIDVLKSGSTEAQEHVIGALFSLAVEEENK 322

Query: 123 MAIGVLGALQPLLYALR-SESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMVKS 182
           M IGVLGA++PLL+ALR SESER R D+AL LY+L++I +NR +LVK GA   +LSM++S
Sbjct: 323 MVIGVLGAVEPLLHALRSSESERARQDAALALYHLSLIPNNRSRLVKAGAVPMMLSMIRS 382

Query: 183 GNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTK--EVNSESTRENCVAALYAL 242
           G S  R+LL+LCN+A C EG+ AMLD +AV  LV  LR      +  + RENCV AL  L
Sbjct: 383 GESASRILLLLCNLAACSEGKGAMLDGNAVSILVGKLRESGGAESDAAARENCVGALLTL 442

Query: 243 SFGSMRFRGLAKEARAMEVLREIV--DGGSERAREKAKMILQRMKG 284
           S G+MRFRGLA EA A E+L EIV  + GS R +EKA  ILQ ++G
Sbjct: 443 SVGNMRFRGLASEAGAEEILTEIVESESGSGRLKEKASKILQTLRG 488

BLAST of MS001945 vs. ExPASy Swiss-Prot
Match: Q8VZ40 (U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.0e-33
Identity = 93/272 (34.19%), Postives = 155/272 (56.99%), Query Frame = 0

Query: 10  LLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRYSTVQINAVA 69
           LL KL +    +Q      LR + K +   RV +     +  L  L++S     Q ++V 
Sbjct: 350 LLEKLANGTTEQQRAAAGELRLLAKRNVDNRVCIAEAGAIPLLVELLSSPDPRTQEHSVT 409

Query: 70  SLVNLSLERRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDENRMAIGVLG 129
           +L+NLS+   NK  IV +G + ++++VLK G  E++E+AA  LFSL++ DEN++AIG  G
Sbjct: 410 ALLNLSINEGNKGAIVDAGAITDIVEVLKNGSMEARENAAATLFSLSVIDENKVAIGAAG 469

Query: 130 ALQPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMVK--SGNSVDR 189
           A+Q L+  L   + R + D+A  ++NL + Q N+ + VK G    L  ++K   G  VD 
Sbjct: 470 AIQALISLLEEGTRRGKKDAATAIFNLCIYQGNKSRAVKGGIVDPLTRLLKDAGGGMVDE 529

Query: 190 LLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTKEVNSESTRENCVAALYALSFGSMRFR 249
            L IL  ++   EG++A+ +A+++  LVE++RT    S   REN  A L+ L  G++   
Sbjct: 530 ALAILAILSTNQEGKTAIAEAESIPVLVEIIRT---GSPRNRENAAAILWYLCIGNIERL 589

Query: 250 GLAKEARAMEVLREIVDGGSERAREKAKMILQ 280
            +A+E  A   L+E+ + G++RA+ KA  +L+
Sbjct: 590 NVAREVGADVALKELTENGTDRAKRKAASLLE 618

BLAST of MS001945 vs. ExPASy TrEMBL
Match: A0A6J1C194 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111007454 PE=4 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 3.9e-149
Identity = 291/292 (99.66%), Postives = 292/292 (100.00%), Query Frame = 0

Query: 1   SSISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRY 60
           SSISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRY
Sbjct: 214 SSISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRY 273

Query: 61  STVQINAVASLVNLSLERRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDE 120
           STVQINAVASLVNLSLE+RNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDE
Sbjct: 274 STVQINAVASLVNLSLEKRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDE 333

Query: 121 NRMAIGVLGALQPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMVK 180
           NRMAIGVLGALQPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMVK
Sbjct: 334 NRMAIGVLGALQPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMVK 393

Query: 181 SGNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTKEVNSESTRENCVAALYALS 240
           SGNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTKEVNSESTRENCVAALYALS
Sbjct: 394 SGNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTKEVNSESTRENCVAALYALS 453

Query: 241 FGSMRFRGLAKEARAMEVLREIVDGGSERAREKAKMILQRMKGRFDGDDEEE 293
           FGSMRFRGLAKEARAMEVLREIVDGGSERAREKAKMILQRMKGRFDGDDEEE
Sbjct: 454 FGSMRFRGLAKEARAMEVLREIVDGGSERAREKAKMILQRMKGRFDGDDEEE 505

BLAST of MS001945 vs. ExPASy TrEMBL
Match: A0A5A7URV9 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G001940 PE=4 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 1.2e-121
Identity = 244/294 (82.99%), Postives = 269/294 (91.50%), Query Frame = 0

Query: 1   SSISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRY 60
           SSISE+E+  L+KL+SP+VF+QEEGV+SLRKITKADE IRVSLCTPRILF+L  LI SRY
Sbjct: 209 SSISEDEKNFLTKLESPDVFQQEEGVVSLRKITKADENIRVSLCTPRILFSLHRLIKSRY 268

Query: 61  STVQINAVASLVNLSLERRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDE 120
             VQINAVASLVNLSLE+ NK+KI RSGLVP+LIDVLKGGH+E+QEHAAGALFSLALEDE
Sbjct: 269 PKVQINAVASLVNLSLEKPNKLKIARSGLVPDLIDVLKGGHSEAQEHAAGALFSLALEDE 328

Query: 121 NRMAIGVLGALQPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMVK 180
           NRMAIGVLGAL PLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGA TTLLSMVK
Sbjct: 329 NRMAIGVLGALPPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAVTTLLSMVK 388

Query: 181 SGNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTKEVNSESTRENCVAALYALS 240
           S NS +RLLLILCN+AVC EGRSAMLDA+AV  LV MLR KE+NSESTRENCVAALYALS
Sbjct: 389 SRNSTNRLLLILCNMAVCQEGRSAMLDANAVELLVGMLREKELNSESTRENCVAALYALS 448

Query: 241 FGSMRFRGLAKEARAMEVLREIVDGGSERAREKAKMILQRM--KGRFDGDDEEE 293
           +GSMRF+GLAKEA AMEVLREIV+ GSERAREKAK IL+RM  +GRFD DD+++
Sbjct: 449 YGSMRFKGLAKEAGAMEVLREIVERGSERAREKAKKILERMRTRGRFDEDDDDD 502

BLAST of MS001945 vs. ExPASy TrEMBL
Match: A0A5D3BFQ0 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G002020 PE=4 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 4.4e-121
Identity = 243/294 (82.65%), Postives = 268/294 (91.16%), Query Frame = 0

Query: 1   SSISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRY 60
           SSISE+E+  L+KL+SP+VF+QEEGV+SLRKITKADE IRVSLCTPRILF+L  LI SRY
Sbjct: 209 SSISEDEKNFLTKLESPDVFQQEEGVVSLRKITKADENIRVSLCTPRILFSLHRLIKSRY 268

Query: 61  STVQINAVASLVNLSLERRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDE 120
             VQINAVASLVNLSLE+ NK+KI RSGLVP+LIDVLKGGH+E+QEHAAGALFSLALEDE
Sbjct: 269 PKVQINAVASLVNLSLEKPNKLKIARSGLVPDLIDVLKGGHSEAQEHAAGALFSLALEDE 328

Query: 121 NRMAIGVLGALQPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMVK 180
           NRMAIGVLGAL PLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGA T LLSMVK
Sbjct: 329 NRMAIGVLGALPPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAVTNLLSMVK 388

Query: 181 SGNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTKEVNSESTRENCVAALYALS 240
           S NS +RLLLILCN+AVC EGRSAMLDA+AV  LV MLR KE+NSESTRENCVAALYALS
Sbjct: 389 SRNSTNRLLLILCNMAVCQEGRSAMLDANAVELLVGMLREKELNSESTRENCVAALYALS 448

Query: 241 FGSMRFRGLAKEARAMEVLREIVDGGSERAREKAKMILQRM--KGRFDGDDEEE 293
           +GSMRF+GLAKEA AMEVLREIV+ GSERAREKAK IL+RM  +GRFD DD+++
Sbjct: 449 YGSMRFKGLAKEAGAMEVLREIVERGSERAREKAKKILERMRTRGRFDEDDDDD 502

BLAST of MS001945 vs. ExPASy TrEMBL
Match: A0A1S3BVH8 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103493938 PE=4 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 4.4e-121
Identity = 243/294 (82.65%), Postives = 268/294 (91.16%), Query Frame = 0

Query: 1   SSISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRY 60
           SSISE+E+  L+KL+SP+VF+QEEGV+SLRKITKADE IRVSLCTPRILF+L  LI SRY
Sbjct: 209 SSISEDEKNFLTKLESPDVFQQEEGVVSLRKITKADENIRVSLCTPRILFSLHRLIKSRY 268

Query: 61  STVQINAVASLVNLSLERRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDE 120
             VQINAVASLVNLSLE+ NK+KI RSGLVP+LIDVLKGGH+E+QEHAAGALFSLALEDE
Sbjct: 269 PKVQINAVASLVNLSLEKPNKLKIARSGLVPDLIDVLKGGHSEAQEHAAGALFSLALEDE 328

Query: 121 NRMAIGVLGALQPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMVK 180
           NRMAIGVLGAL PLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGA T LLSMVK
Sbjct: 329 NRMAIGVLGALPPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAVTNLLSMVK 388

Query: 181 SGNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTKEVNSESTRENCVAALYALS 240
           S NS +RLLLILCN+AVC EGRSAMLDA+AV  LV MLR KE+NSESTRENCVAALYALS
Sbjct: 389 SRNSTNRLLLILCNMAVCQEGRSAMLDANAVELLVGMLREKELNSESTRENCVAALYALS 448

Query: 241 FGSMRFRGLAKEARAMEVLREIVDGGSERAREKAKMILQRM--KGRFDGDDEEE 293
           +GSMRF+GLAKEA AMEVLREIV+ GSERAREKAK IL+RM  +GRFD DD+++
Sbjct: 449 YGSMRFKGLAKEAGAMEVLREIVERGSERAREKAKKILERMRTRGRFDEDDDDD 502

BLAST of MS001945 vs. ExPASy TrEMBL
Match: A0A6J1EFK0 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432065 PE=4 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 2.1e-118
Identity = 241/294 (81.97%), Postives = 264/294 (89.80%), Query Frame = 0

Query: 1   SSISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRY 60
           SSIS++E+ELLSKL SP+VFKQEEGVISLRKITK +EKIRVSLCTPRIL +LR LITSRY
Sbjct: 213 SSISQDEKELLSKLTSPDVFKQEEGVISLRKITKENEKIRVSLCTPRILLSLRPLITSRY 272

Query: 61  STVQINAVASLVNLSLERRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDE 120
           S VQINA+ASLVNLSLE+ NK KI RSGLVPNLID LKGGH E+QEHAAGALFSLALEDE
Sbjct: 273 SAVQINALASLVNLSLEKLNKQKIARSGLVPNLIDALKGGHAEAQEHAAGALFSLALEDE 332

Query: 121 NRMAIGVLGALQPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMVK 180
           NRM IGVLGAL PLLYALRS SE TR DSALCLYNLTMIQSNRVKL+KLGA TTLLSMVK
Sbjct: 333 NRMTIGVLGALPPLLYALRSNSEITRKDSALCLYNLTMIQSNRVKLIKLGAVTTLLSMVK 392

Query: 181 SGNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTKEVNSESTRENCVAALYALS 240
           SG+S++ LLLILCNIAVC EGRSAMLDA+AVG LV MLR KE+ S+STRENCVAALYALS
Sbjct: 393 SGSSMNWLLLILCNIAVCQEGRSAMLDANAVGLLVGMLREKELYSKSTRENCVAALYALS 452

Query: 241 FGSMRFRGLAKEARAMEVLREIVDGGSERAREKAKMILQRMK--GRFDGDDEEE 293
           +GSMRF+GLAKEA AMEVLREI + GS+RAREKAK IL+RMK  G F+ DD+EE
Sbjct: 453 YGSMRFKGLAKEAGAMEVLREIAERGSDRAREKAKKILERMKMRGTFNEDDDEE 506

BLAST of MS001945 vs. TAIR 10
Match: AT5G65200.1 (plant U-box 38 )

HSP 1 Score: 334.7 bits (857), Expect = 7.3e-92
Identity = 186/300 (62.00%), Postives = 237/300 (79.00%), Query Frame = 0

Query: 1   SSISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRY 60
           S+ +EE+E + +KLKS E+F QE+G+I +RK+T+ +++ RVSLC+PRIL  L+ +I SRY
Sbjct: 220 STATEEDEVIYNKLKSSEIFDQEQGLIMMRKMTRTNDEARVSLCSPRILSLLKNMIVSRY 279

Query: 61  STVQINAVASLVNLSLERRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDE 120
           S VQ NA+ASLVNLSL+++NK+ IVR G VP LIDVLK G  E+QEHAAG +FSL+LED+
Sbjct: 280 SLVQTNALASLVNLSLDKKNKLTIVRLGFVPILIDVLKSGSREAQEHAAGTIFSLSLEDD 339

Query: 121 NRMAIGVLGALQPLLYALR-SESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMV 180
           N+M IGVLGALQPLL+ALR +ES+RTR DSAL LY+LT+ Q+NR KLV+LGA   L SMV
Sbjct: 340 NKMPIGVLGALQPLLHALRAAESDRTRHDSALALYHLTLNQTNRSKLVRLGAVPALFSMV 399

Query: 181 KSGNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLR-------TKEVNSESTRENC 240
           +SG S  R LL++CN+A C EGRSAMLDA+AV  LV  LR       T+  +S S RENC
Sbjct: 400 RSGESASRALLVICNLACCSEGRSAMLDANAVAILVGKLREEWTEEPTEARSSSSARENC 459

Query: 241 VAALYALSFGSMRFRGLAKEARAMEVLREIVDGGSERAREKAKMILQRMKGRFDGDDEEE 293
           VAAL+ALS  S+RF+GLAKEARA+EVL+E+ + G+ERAREKAK ILQ M+ R   DDEE+
Sbjct: 460 VAALFALSHESLRFKGLAKEARAVEVLKEVEERGTERAREKAKKILQLMRERVPEDDEED 519

BLAST of MS001945 vs. TAIR 10
Match: AT5G62560.1 (RING/U-box superfamily protein with ARM repeat domain )

HSP 1 Score: 321.2 bits (822), Expect = 8.4e-88
Identity = 180/294 (61.22%), Postives = 226/294 (76.87%), Query Frame = 0

Query: 1   SSISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRY 60
           S +S EEEE+ +KL+  ++F  E+G+I LRK+T++ E +RVSLCT RIL  LR L+ SRY
Sbjct: 230 SPMSPEEEEIFNKLRGTDIFDHEQGLILLRKMTRSSEDLRVSLCTDRILSFLRSLLVSRY 289

Query: 61  STVQINAVASLVNLSLERRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDE 120
           + VQ NA AS+VNLSLE++NKVKIVRSG VP LIDVLK G TE+QEH AGALFSLALEDE
Sbjct: 290 NLVQTNAAASVVNLSLEKQNKVKIVRSGFVPLLIDVLKSGTTEAQEHVAGALFSLALEDE 349

Query: 121 NRMAIGVLGALQPLLYALR-SESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMV 180
           N+M IGVLGA++PLL+ALR SESER R D+AL LY+L++I SNR +LV+ GA  TLLSMV
Sbjct: 350 NKMVIGVLGAVEPLLHALRSSESERARQDAALALYHLSLIPSNRTRLVRAGAVPTLLSMV 409

Query: 181 KSGNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTKEV---NSESTRENCVAAL 240
           +SG+S  R+LL+LCN+A CP+G+ AMLD +AV  LV  LR  EV   +SE+ RENCVA L
Sbjct: 410 RSGDSTSRILLVLCNLAACPDGKGAMLDGNAVAILVGKLR--EVGGGDSEAARENCVAVL 469

Query: 241 YALSFGSMRFRGLAKEARAMEVLREIVDGGSERAREKAKMILQRMKGRFDGDDE 291
             L  G++RFRGLA EA A EVL E+ + G+ER +EKA  IL  M+G   G+ E
Sbjct: 470 LTLCQGNLRFRGLASEAGAEEVLMEVEENGNERVKEKASKILLAMRGGGGGESE 521

BLAST of MS001945 vs. TAIR 10
Match: AT5G40140.1 (RING/U-box superfamily protein with ARM repeat domain )

HSP 1 Score: 300.4 bits (768), Expect = 1.5e-81
Identity = 163/289 (56.40%), Postives = 216/289 (74.74%), Query Frame = 0

Query: 2   SISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRYS 61
           +++ EEE LL+KLKS  + + EE +IS+R+IT+ DE  R+SLCT R++ AL+ LI SRY+
Sbjct: 225 NLTPEEEALLTKLKSNRISEIEEALISIRRITRIDESSRISLCTTRVISALKSLIVSRYA 284

Query: 62  TVQINAVASLVNLSLERRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDEN 121
           TVQ+N  A LVNLSLE+ NKVKIVRSG+VP LIDVLK G  E+QEH+AG +FSLALEDEN
Sbjct: 285 TVQVNVTAVLVNLSLEKSNKVKIVRSGIVPPLIDVLKCGSVEAQEHSAGVIFSLALEDEN 344

Query: 122 RMAIGVLGALQPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMVKS 181
           + AIGVLG L+PLL+ +R  +E TR DSAL LY+L+++QSNR KLVKLGA   LL MV  
Sbjct: 345 KTAIGVLGGLEPLLHLIRVGTELTRHDSALALYHLSLVQSNRGKLVKLGAVQMLLGMVSL 404

Query: 182 GNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTKEVNSESTRENCVAALYALSF 241
           G  + R+LLILCN+A CP  R A+LD+  V C+V +LR     +ESTRE+CVA LY LS 
Sbjct: 405 GQMIGRVLLILCNMASCPVSRPALLDSGGVECMVGVLRRDREVNESTRESCVAVLYGLSH 464

Query: 242 -GSMRFRGLAKEARAMEVLREIVDGGSERAREKAKMILQRMKGRFDGDD 290
            G +RF+GLA  A A+E L ++   G ERA++KA+ +L+ ++ + + DD
Sbjct: 465 DGGLRFKGLAMAANAVEELVKVERSGRERAKQKARRVLEVLRAKIEDDD 513

BLAST of MS001945 vs. TAIR 10
Match: AT3G47820.1 (PLANT U-BOX 39 )

HSP 1 Score: 299.3 bits (765), Expect = 3.4e-81
Identity = 169/286 (59.09%), Postives = 212/286 (74.13%), Query Frame = 0

Query: 3   ISEEEEELLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRYST 62
           +S EEEE+ +KL S +    E+G+I LRK T+++E  R+SLCT RIL  LR LI SRY+ 
Sbjct: 181 MSPEEEEIYNKLTSVDTIDHEQGLIQLRKTTRSNETTRISLCTDRILSLLRSLIVSRYNI 240

Query: 63  VQINAVASLVNLSLERRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDENR 122
           VQ NA AS+VNLSLE+ NK+KIVRSG VP LIDVLK G TE+QEH  GALFSLA+E+EN+
Sbjct: 241 VQTNAAASIVNLSLEKPNKLKIVRSGFVPLLIDVLKSGSTEAQEHVIGALFSLAVEEENK 300

Query: 123 MAIGVLGALQPLLYALR-SESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMVKS 182
           M IGVLGA++PLL+ALR SESER R D+AL LY+L++I +NR +LVK GA   +LSM++S
Sbjct: 301 MVIGVLGAVEPLLHALRSSESERARQDAALALYHLSLIPNNRSRLVKAGAVPMMLSMIRS 360

Query: 183 GNSVDRLLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTK--EVNSESTRENCVAALYAL 242
           G S  R+LL+LCN+A C EG+ AMLD +AV  LV  LR      +  + RENCV AL  L
Sbjct: 361 GESASRILLLLCNLAACSEGKGAMLDGNAVSILVGKLRESGGAESDAAARENCVGALLTL 420

Query: 243 SFGSMRFRGLAKEARAMEVLREIV--DGGSERAREKAKMILQRMKG 284
           S G+MRFRGLA EA A E+L EIV  + GS R +EKA  ILQ ++G
Sbjct: 421 SVGNMRFRGLASEAGAEEILTEIVESESGSGRLKEKASKILQTLRG 466

BLAST of MS001945 vs. TAIR 10
Match: AT3G54850.1 (plant U-box 14 )

HSP 1 Score: 144.4 bits (363), Expect = 1.4e-34
Identity = 93/272 (34.19%), Postives = 155/272 (56.99%), Query Frame = 0

Query: 10  LLSKLKSPEVFKQEEGVISLRKITKADEKIRVSLCTPRILFALRLLITSRYSTVQINAVA 69
           LL KL +    +Q      LR + K +   RV +     +  L  L++S     Q ++V 
Sbjct: 350 LLEKLANGTTEQQRAAAGELRLLAKRNVDNRVCIAEAGAIPLLVELLSSPDPRTQEHSVT 409

Query: 70  SLVNLSLERRNKVKIVRSGLVPNLIDVLKGGHTESQEHAAGALFSLALEDENRMAIGVLG 129
           +L+NLS+   NK  IV +G + ++++VLK G  E++E+AA  LFSL++ DEN++AIG  G
Sbjct: 410 ALLNLSINEGNKGAIVDAGAITDIVEVLKNGSMEARENAAATLFSLSVIDENKVAIGAAG 469

Query: 130 ALQPLLYALRSESERTRDDSALCLYNLTMIQSNRVKLVKLGAATTLLSMVK--SGNSVDR 189
           A+Q L+  L   + R + D+A  ++NL + Q N+ + VK G    L  ++K   G  VD 
Sbjct: 470 AIQALISLLEEGTRRGKKDAATAIFNLCIYQGNKSRAVKGGIVDPLTRLLKDAGGGMVDE 529

Query: 190 LLLILCNIAVCPEGRSAMLDADAVGCLVEMLRTKEVNSESTRENCVAALYALSFGSMRFR 249
            L IL  ++   EG++A+ +A+++  LVE++RT    S   REN  A L+ L  G++   
Sbjct: 530 ALAILAILSTNQEGKTAIAEAESIPVLVEIIRT---GSPRNRENAAAILWYLCIGNIERL 589

Query: 250 GLAKEARAMEVLREIVDGGSERAREKAKMILQ 280
            +A+E  A   L+E+ + G++RA+ KA  +L+
Sbjct: 590 NVAREVGADVALKELTENGTDRAKRKAASLLE 618

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135520.18.0e-14999.66U-box domain-containing protein 38 [Momordica charantia][more]
XP_038879975.11.4e-12485.71U-box domain-containing protein 38-like [Benincasa hispida][more]
KAA0057894.12.4e-12182.99U-box domain-containing protein 38 [Cucumis melo var. makuwa][more]
TYJ98582.19.2e-12182.65U-box domain-containing protein 38 [Cucumis melo var. makuwa][more]
XP_008453132.19.2e-12182.65PREDICTED: U-box domain-containing protein 38 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9FJP61.0e-9062.00U-box domain-containing protein 38 OS=Arabidopsis thaliana OX=3702 GN=PUB38 PE=1... [more]
Q0WUF61.2e-8661.22U-box domain-containing protein 41 OS=Arabidopsis thaliana OX=3702 GN=PUB41 PE=2... [more]
Q9FL172.2e-8056.40U-box domain-containing protein 40 OS=Arabidopsis thaliana OX=3702 GN=PUB40 PE=2... [more]
Q9STT14.8e-8059.09U-box domain-containing protein 39 OS=Arabidopsis thaliana OX=3702 GN=PUB39 PE=1... [more]
Q8VZ402.0e-3334.19U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1... [more]
Match NameE-valueIdentityDescription
A0A6J1C1943.9e-14999.66RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11100745... [more]
A0A5A7URV91.2e-12182.99RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A5D3BFQ04.4e-12182.65RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BVH84.4e-12182.65RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103493938 PE=4 ... [more]
A0A6J1EFK02.1e-11881.97RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432065... [more]
Match NameE-valueIdentityDescription
AT5G65200.17.3e-9262.00plant U-box 38 [more]
AT5G62560.18.4e-8861.22RING/U-box superfamily protein with ARM repeat domain [more]
AT5G40140.11.5e-8156.40RING/U-box superfamily protein with ARM repeat domain [more]
AT3G47820.13.4e-8159.09PLANT U-BOX 39 [more]
AT3G54850.11.4e-3434.19plant U-box 14 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 287..292
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 2..291
NoneNo IPR availablePANTHERPTHR23315:SF276U-BOX DOMAIN-CONTAINING PROTEIN 38coord: 2..291
IPR000225ArmadilloSMARTSM00185arm_5coord: 198..241
e-value: 0.11
score: 21.6
coord: 77..117
e-value: 8.8
score: 13.7
coord: 118..158
e-value: 86.0
score: 6.0
IPR000225ArmadilloPFAMPF00514Armcoord: 119..157
e-value: 1.7E-4
score: 21.5
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 88..125
score: 9.2224
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 5..281
e-value: 2.9E-41
score: 143.4
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 9..281

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS001945.1MS001945.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding