MS001943 (gene) Bitter gourd (TR) v1

BLAST of MS001943 vs. NCBI nr
Match: XP_022135212.1 (alpha-mannosidase I MNS5 isoform X1 [Momordica charantia])

HSP 1 Score: 1174.8 bits (3038), Expect = 0.0e+00
Identity = 573/588 (97.45%), Postives = 575/588 (97.79%), Query Frame = 0

Query: 1   MLPCRSATWILLLFFIFSALFGPSTSDDYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60
           MLPCRSATWILLLFFIFSALFGPSTSD+YWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD
Sbjct: 1   MLPCRSATWILLLFFIFSALFGPSTSDNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60

Query: 61  ELKPLTKTFTDSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120
           ELKPLTKTFTDSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE
Sbjct: 61  ELKPLTKTFTDSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120

Query: 121 NLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQNYKNQLLVLAEDLGNRLLPA 180
           NLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQNYKNQLLVLAEDLGNRLLPA
Sbjct: 121 NLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQNYKNQLLVLAEDLGNRLLPA 180

Query: 181 FNTPTGLPYAWINLKHGVMENETTETSTSGCGSLILEMGALSRLTGDPRFESAALRALRK 240
           FNTPTGLPYAWINLKHGVMENETTETSTSGCGSLILEMGALSRLTGDPRFESAALRALRK
Sbjct: 181 FNTPTGLPYAWINLKHGVMENETTETSTSGCGSLILEMGALSRLTGDPRFESAALRALRK 240

Query: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY 300
           LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY
Sbjct: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY 300

Query: 301 LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE 360
           LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE
Sbjct: 301 LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE 360

Query: 361 KYGVLPERHAASKSIYISYPVYLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE 420
           KYGVLPER             YLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE
Sbjct: 361 KYGVLPER-------------YLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE 420

Query: 421 VGESIMKSLVLYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY 480
           VGESIMKSLVLYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY
Sbjct: 421 VGESIMKSLVLYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY 480

Query: 481 IFTTEGHPLPVLSSWHERLPEVYGLANGTSIKGENSSRRLSAMSLQVCPATSLNTAGDGG 540
           IFTTEGHPLPVLSSWHERLPEVYGLANGTSIKGENSSRRLSAMSLQVCPA+SLNTAGDGG
Sbjct: 481 IFTTEGHPLPVLSSWHERLPEVYGLANGTSIKGENSSRRLSAMSLQVCPASSLNTAGDGG 540

Query: 541 QPIESACHILDARADHRCFSDEECGVDSTTCRRRSCSTSGYCGLWLHI 589
           QPIESACHILDARADHRCFSDEECGVDSTTCRRRSCSTSGYCGLWLHI
Sbjct: 541 QPIESACHILDARADHRCFSDEECGVDSTTCRRRSCSTSGYCGLWLHI 575

BLAST of MS001943 vs. NCBI nr
Match: XP_038879418.1 (alpha-mannosidase I MNS5 [Benincasa hispida])

HSP 1 Score: 1126.7 bits (2913), Expect = 0.0e+00
Identity = 550/587 (93.70%), Postives = 561/587 (95.57%), Query Frame = 0

Query: 1   MLPCRSATWILLLFFIFSALFGPSTSDDYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60
           MLP RS TWILLLF IFSALF PS+S +YWTAKRMRMREKVRRMFYHAYDNYMTYAFP+D
Sbjct: 1   MLPRRSFTWILLLFIIFSALFNPSSSSNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPND 60

Query: 61  ELKPLTKTFTDSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120
           ELKPLTKTFT+SLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE
Sbjct: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120

Query: 121 NLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQNYKNQLLVLAEDLGNRLLPA 180
           NLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQNYKNQLLVLAEDLGNR LPA
Sbjct: 121 NLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQNYKNQLLVLAEDLGNRFLPA 180

Query: 181 FNTPTGLPYAWINLKHGVMENETTETSTSGCGSLILEMGALSRLTGDPRFESAALRALRK 240
           FNTPTGLPYAWINLKHGVME+ETTETSTSGCGSLILEMGALSRLTGDPRFE AAL ALRK
Sbjct: 181 FNTPTGLPYAWINLKHGVMEDETTETSTSGCGSLILEMGALSRLTGDPRFEYAALHALRK 240

Query: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY 300
           LWS+RSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY
Sbjct: 241 LWSLRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY 300

Query: 301 LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE 360
           LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE
Sbjct: 301 LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE 360

Query: 361 KYGVLPERHAASKSIYISYPVYLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE 420
           KYGVLPER             YLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE
Sbjct: 361 KYGVLPER-------------YLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE 420

Query: 421 VGESIMKSLVLYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY 480
           VGESIMKSL+LYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLV QNY
Sbjct: 421 VGESIMKSLILYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVGQNY 480

Query: 481 IFTTEGHPLPVLSSWHERLPEVYGLANGTSIKGENSSRRLSAMSLQVCPATSLNTAGDGG 540
           IFTTEGHPLPVLSSWHE+LPEVYGL NGTSIKGENSSRRLSAMSLQVCPATSLNTAGDG 
Sbjct: 481 IFTTEGHPLPVLSSWHEQLPEVYGLTNGTSIKGENSSRRLSAMSLQVCPATSLNTAGDGS 540

Query: 541 QPIESACHILDARADHRCFSDEECGVDSTTCRRRSCSTSGYCGLWLH 588
           Q IESACHILDARADH+CFSDEECGVDSTTCRRRSCS+SGYCG WL+
Sbjct: 541 QQIESACHILDARADHKCFSDEECGVDSTTCRRRSCSSSGYCGQWLY 574

BLAST of MS001943 vs. NCBI nr
Match: XP_023516302.1 (alpha-mannosidase I MNS5 [Cucurbita pepo subsp. pepo] >KAG7023152.1 Alpha-mannosidase I MNS5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1121.7 bits (2900), Expect = 0.0e+00
Identity = 547/588 (93.03%), Postives = 562/588 (95.58%), Query Frame = 0

Query: 1   MLPCRSATWILLLFFIFSALFGPSTSDDYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60
           MLP RS TWILLLF IFSALF PS+S++YWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD
Sbjct: 1   MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60

Query: 61  ELKPLTKTFTDSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120
           ELKPLTKTFT+SLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE
Sbjct: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120

Query: 121 NLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQNYKNQLLVLAEDLGNRLLPA 180
           NL FDVDARINLFECNIRVLGGLVSAHVLATDSTNRL RQ+YKNQLLVLAEDLGNR LPA
Sbjct: 121 NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRQSYKNQLLVLAEDLGNRFLPA 180

Query: 181 FNTPTGLPYAWINLKHGVMENETTETSTSGCGSLILEMGALSRLTGDPRFESAALRALRK 240
           FNTPTGLPYAWINLKHGVMENETTETS+SGCGSLILEMGALSRLTGDPRFESAALRALRK
Sbjct: 181 FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRK 240

Query: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY 300
           LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFH+AY
Sbjct: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAY 300

Query: 301 LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE 360
           LAVQKYFRHGPWYHEADMRTGRAT+WQLTSLQAFWPGLQVLVGDIPAANSSHREFF+VWE
Sbjct: 301 LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE 360

Query: 361 KYGVLPERHAASKSIYISYPVYLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE 420
           KYGVLPER             YLLD+QMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE
Sbjct: 361 KYGVLPER-------------YLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE 420

Query: 421 VGESIMKSLVLYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY 480
           VGESIMKSL+LYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY
Sbjct: 421 VGESIMKSLILYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY 480

Query: 481 IFTTEGHPLPVLSSWHERLPEVYGLANGTSIKGENSSRRLSAMSLQVCPATSLNTAGDGG 540
           IFTTEGHPLPVLSSWHERLPEVYGL NGTSIKGENSSRR SAMSLQVCPATSLNTAGDG 
Sbjct: 481 IFTTEGHPLPVLSSWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGD 540

Query: 541 QPIESACHILDARADHRCFSDEECGVDSTTCRRRSCSTSGYCGLWLHI 589
           Q +ESACHILDARADH+CFSDEECGVDSTTCRRRSCS+SGYCG WL +
Sbjct: 541 QQLESACHILDARADHKCFSDEECGVDSTTCRRRSCSSSGYCGHWLRL 575

BLAST of MS001943 vs. NCBI nr
Match: XP_022921842.1 (alpha-mannosidase I MNS5 [Cucurbita moschata] >KAG6589467.1 Alpha-mannosidase I MNS5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 546/588 (92.86%), Postives = 561/588 (95.41%), Query Frame = 0

Query: 1   MLPCRSATWILLLFFIFSALFGPSTSDDYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60
           MLP RS TWILLLF IFSALF PS+S++YWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD
Sbjct: 1   MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60

Query: 61  ELKPLTKTFTDSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120
           ELKPLTKTFT+SLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE
Sbjct: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120

Query: 121 NLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQNYKNQLLVLAEDLGNRLLPA 180
           NL FDVDARINLFECNIRVLGGLVSAHVLATDST RL RQ+YKNQLLVLAEDLGNR LPA
Sbjct: 121 NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTTRLPRQSYKNQLLVLAEDLGNRFLPA 180

Query: 181 FNTPTGLPYAWINLKHGVMENETTETSTSGCGSLILEMGALSRLTGDPRFESAALRALRK 240
           FNTPTGLPYAWINLKHGVMENETTETS+SGCGSLILEMGALSRLTGDPRFESAALRALRK
Sbjct: 181 FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRK 240

Query: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY 300
           LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFH+AY
Sbjct: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAY 300

Query: 301 LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE 360
           LAVQKYFRHGPWYHEADMRTGRAT+WQLTSLQAFWPGLQVLVGDIPAANSSHREFF+VWE
Sbjct: 301 LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE 360

Query: 361 KYGVLPERHAASKSIYISYPVYLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE 420
           KYGVLPER             YLLD+QMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE
Sbjct: 361 KYGVLPER-------------YLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE 420

Query: 421 VGESIMKSLVLYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY 480
           VGESIMKSL+LYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY
Sbjct: 421 VGESIMKSLILYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY 480

Query: 481 IFTTEGHPLPVLSSWHERLPEVYGLANGTSIKGENSSRRLSAMSLQVCPATSLNTAGDGG 540
           IFTTEGHPLPVLSSWHERLPEVYGL NGTSIKGENSSRR SAMSLQVCPATSLNTAGDG 
Sbjct: 481 IFTTEGHPLPVLSSWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGD 540

Query: 541 QPIESACHILDARADHRCFSDEECGVDSTTCRRRSCSTSGYCGLWLHI 589
           Q +ESACHILDARADH+CFSDEECGVDSTTCRRRSCS+SGYCG WL +
Sbjct: 541 QQLESACHILDARADHKCFSDEECGVDSTTCRRRSCSSSGYCGHWLRL 575

BLAST of MS001943 vs. NCBI nr
Match: XP_022988359.1 (alpha-mannosidase I MNS5 [Cucurbita maxima])

HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 545/588 (92.69%), Postives = 562/588 (95.58%), Query Frame = 0

Query: 1   MLPCRSATWILLLFFIFSALFGPSTSDDYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60
           MLP RS TWILLLF IFSALF PS+S++YWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD
Sbjct: 1   MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60

Query: 61  ELKPLTKTFTDSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120
           ELKPLTKTFT+SLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE
Sbjct: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120

Query: 121 NLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQNYKNQLLVLAEDLGNRLLPA 180
           NL FDVDARINLFECNIRVLGGLVSAHVLATDSTNRL R++YKNQLLVLAEDLGNR LPA
Sbjct: 121 NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRESYKNQLLVLAEDLGNRFLPA 180

Query: 181 FNTPTGLPYAWINLKHGVMENETTETSTSGCGSLILEMGALSRLTGDPRFESAALRALRK 240
           FNTPTGLPYAWINLKHGVMENETTETS+SGCGSLILEMGALS+LTGDPRFESAALRALRK
Sbjct: 181 FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSQLTGDPRFESAALRALRK 240

Query: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY 300
           LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY
Sbjct: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY 300

Query: 301 LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE 360
           LAVQKYFRHGPWYHEADMRTGRAT+WQLTSLQAFWPGLQVLVGDIPAANSSHREFF+VWE
Sbjct: 301 LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE 360

Query: 361 KYGVLPERHAASKSIYISYPVYLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE 420
           KYGVLPER             YLLD+QMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE
Sbjct: 361 KYGVLPER-------------YLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE 420

Query: 421 VGESIMKSLVLYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY 480
           VGESIMKSL+LYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY
Sbjct: 421 VGESIMKSLILYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY 480

Query: 481 IFTTEGHPLPVLSSWHERLPEVYGLANGTSIKGENSSRRLSAMSLQVCPATSLNTAGDGG 540
           IFTTEGHPLPVLSSWHERLPEVYGL NGTSIKGENSSRR SAMSLQVCPATSLNTAGDG 
Sbjct: 481 IFTTEGHPLPVLSSWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGD 540

Query: 541 QPIESACHILDARADHRCFSDEECGVDSTTCRRRSCSTSGYCGLWLHI 589
           Q +ESACHILDARADH+CFS+EECGVDSTTCRRRSCS+SGYCG WL +
Sbjct: 541 QQLESACHILDARADHKCFSNEECGVDSTTCRRRSCSSSGYCGHWLRL 575

BLAST of MS001943 vs. ExPASy Swiss-Prot
Match: Q9SXC9 (Alpha-mannosidase I MNS5 OS=Arabidopsis thaliana OX=3702 GN=MNS5 PE=1 SV=1)

HSP 1 Score: 895.2 bits (2312), Expect = 4.0e-259
Identity = 434/576 (75.35%), Postives = 487/576 (84.55%), Query Frame = 0

Query: 10  ILLLFFIFSALFGPSTSDDYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHDELKPLTKTF 69
           + L   I    F    S  +   K+ +MREKVR MFYHAYDNYMTYAFPHDELKPLTK+F
Sbjct: 10  LFLCLLISLTFFVVDPSSQHIEVKKKQMREKVREMFYHAYDNYMTYAFPHDELKPLTKSF 69

Query: 70  TDSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSENLHFDVDAR 129
           TDSLSELGNLKLEHLP +YNGSA+TL+ESLSSL ILGN+TEF+K VLWLSENL FD+DAR
Sbjct: 70  TDSLSELGNLKLEHLPTDYNGSAVTLVESLSSLAILGNSTEFEKGVLWLSENLTFDIDAR 129

Query: 130 INLFECNIRVLGGLVSAHVLATDSTNRLARQNYKNQLLVLAEDLGNRLLPAFNTPTGLPY 189
           +NLFECNIRVLGGL+SAH+LA D  NRL + +Y NQLL LAEDLG R LPAF TPTGLPY
Sbjct: 130 VNLFECNIRVLGGLISAHLLAIDPNNRLIQGSYNNQLLRLAEDLGKRFLPAFETPTGLPY 189

Query: 190 AWINLKHGVMENETTETSTSGCGSLILEMGALSRLTGDPRFESAALRALRKLWSMRSSLN 249
           AWINLK+GVMENETTETSTSGCGSL+LEMGALSRLTGDPRFESAALRALR+LW MRSSL+
Sbjct: 190 AWINLKNGVMENETTETSTSGCGSLVLEMGALSRLTGDPRFESAALRALRQLWRMRSSLD 249

Query: 250 LLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAYLAVQKYFRH 309
           LLGTTLDV TGEWIE+SS IGAGVDSFYEYL KA++LFGKE++WRMFH+AYLA QKYFRH
Sbjct: 250 LLGTTLDVVTGEWIEYSSSIGAGVDSFYEYLLKAYILFGKEDYWRMFHSAYLASQKYFRH 309

Query: 310 GPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWEKYGVLPERH 369
           GPWYHEA+M +G+ TYWQLTSLQAFWPGLQVLVGDI AANSSHREFF+VWEK+GVLPER 
Sbjct: 310 GPWYHEANMWSGKPTYWQLTSLQAFWPGLQVLVGDIAAANSSHREFFHVWEKFGVLPER- 369

Query: 370 AASKSIYISYPVYLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSL 429
                       YLLDHQ++HPT KYYPLRPELAESTFYLYQATKDPWY +VGES++KSL
Sbjct: 370 ------------YLLDHQIIHPTMKYYPLRPELAESTFYLYQATKDPWYLDVGESMVKSL 429

Query: 430 VLYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPL 489
            LYTKV GGFASVRDVTTMQLEDHQHSFFL+ETCKYLYLLFDDSF+  +NYIFTTEGHP+
Sbjct: 430 NLYTKVPGGFASVRDVTTMQLEDHQHSFFLAETCKYLYLLFDDSFVAKRNYIFTTEGHPI 489

Query: 490 PVLSSWHERLPEVYGLANGTSIKGENSSRRLSAMSLQVCPATSLNTAGDGGQPIESACHI 549
            V+SSWHE+LPE Y   N T  K      R SA+SLQVCP  SLN+     Q  ESACH+
Sbjct: 490 QVVSSWHEKLPETYFSGNWTLSKSGAWESRASALSLQVCPLISLNSR-HPEQQRESACHV 549

Query: 550 LDARADHRCFSDEECGVDSTTCRRRSCSTSGYCGLW 586
           LD + +H+C+S++ECGVD+TTCR R+CS  GYCGLW
Sbjct: 550 LDEQINHKCWSNKECGVDATTCRLRTCSGVGYCGLW 571

BLAST of MS001943 vs. ExPASy Swiss-Prot
Match: Q9FG93 (Alpha-mannosidase I MNS4 OS=Arabidopsis thaliana OX=3702 GN=MNS4 PE=1 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 4.3e-112
Identity = 213/463 (46.00%), Postives = 294/463 (63.50%), Query Frame = 0

Query: 36  RMREKVRRMFYHAYDNYMTYAFPHDELKPLTKTFTDSLSELGNLKLEHLPQNYNGSALTL 95
           ++R++VR MFYHA+D YM  AFP DEL+PL+    D+L                G ALTL
Sbjct: 39  QLRDEVRGMFYHAFDGYMNNAFPLDELRPLSCQGEDTL---------------GGYALTL 98

Query: 96  IESLSSLVILGNNTEFQKAVLWLSENLHFDVDARINLFECNIRVLGGLVSAHVLATDSTN 155
           I+SL +L +LG+   F  +V W+ +NL F+++  +++FE  IRVLGGL+SAH++A+D   
Sbjct: 99  IDSLDTLALLGDRERFTSSVEWIGKNLQFNINKTVSVFETTIRVLGGLLSAHLIASDYAT 158

Query: 156 RLARQNYKNQLLVLAEDLGNRLLPAFNTPTGLPYAWINLKHGVMENETTETSTSGCGSLI 215
            +   +Y N+LLVLAE+L  R+LPAF+TPTG+P+  +NL +GV ++E+  TST+G G+L 
Sbjct: 159 GMRIPSYNNELLVLAENLARRMLPAFDTPTGIPFGSVNLMYGVDKHESKITSTAGGGTLS 218

Query: 216 LEMGALSRLTGDPRFESAALRALRKLWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDS 275
           LE G LSRLT DP FE  A  A+R LW+ RS+L+L+G  ++V TGEW +  +GIG  +DS
Sbjct: 219 LEFGVLSRLTNDPVFEQVAKNAVRGLWARRSNLDLVGAHINVFTGEWTQKDAGIGTSIDS 278

Query: 276 FYEYLFKAHVLFGKEEFWRMFHTAYLAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFW 335
           FYEYL KA++LFG EE+  +F  AY +  +Y    PWY E +M +    +    SLQAFW
Sbjct: 279 FYEYLLKAYILFGDEEYLYIFQEAYRSAMQYLHKDPWYVEVNMDSAAIVWPVFNSLQAFW 338

Query: 336 PGLQVLVGDIPAANSSHREFFYVWEKYGVLPERHAASKSIYISYPVYLLDHQMLHPTEKY 395
           PGLQVL GD+  A  +H  FF VW++YG  PE    + ++ + Y             +K 
Sbjct: 339 PGLQVLAGDVDPAIRTHTAFFSVWKRYGFTPEGFNLA-TLSVQY------------GQKS 398

Query: 396 YPLRPELAESTFYLYQATKDPWYFEVGESIMKSLVLYTKVEGGFASVRDVTTMQLEDHQH 455
           YPLRPEL EST++LY+AT+DP Y + G   + SL    K   G+  + DV   + EDH  
Sbjct: 399 YPLRPELIESTYWLYKATRDPRYLDAGRDFVASLQYGAKCPCGYCHITDVELHKQEDHME 458

Query: 456 SFFLSETCKYLYLLF----DDSFLVDQ---NYIFTTEGHPLPV 492
           SFFL+ET KYL+LLF    D   LVD     YIF+TEGH LP+
Sbjct: 459 SFFLAETVKYLWLLFDLAVDSDNLVDNGPYKYIFSTEGHLLPI 473

BLAST of MS001943 vs. ExPASy Swiss-Prot
Match: Q92611 (ER degradation-enhancing alpha-mannosidase-like protein 1 OS=Homo sapiens OX=9606 GN=EDEM1 PE=1 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 3.4e-109
Identity = 225/519 (43.35%), Postives = 315/519 (60.69%), Query Frame = 0

Query: 34  RMRMREKVRRMFYHAYDNYMTYAFPHDELKPL-TKTFTDSLSELGNLKLEHLPQNYNGSA 93
           R +MR+  R MF   YDNYM +AFP DEL P+  +       +  NL +  +  NY   +
Sbjct: 127 RAQMRDLARGMFVFGYDNYMAHAFPQDELNPIHCRGRGPDRGDPSNLNINDVLGNY---S 186

Query: 94  LTLIESLSSLVILGNNTEFQKAVLWLSENLHFDVDARINLFECNIRVLGGLVSAHVLATD 153
           LTL+++L +L I+GN++EFQKAV  +   + FD D+ + +FE  IRVLG L+SAH + TD
Sbjct: 187 LTLVDALDTLAIMGNSSEFQKAVKLVINTVSFDKDSTVQVFEATIRVLGSLLSAHRIITD 246

Query: 154 ST---NRLARQNYKNQLLVLAEDLGNRLLPAF-NTPTGLPYAWINLKHGVMENETTETST 213
           S      +  ++Y N+LL +A DL  RLLPAF NT TG+PY  +NLK GV  +   ET T
Sbjct: 247 SKQPFGDMTIKDYDNELLYMAHDLAVRLLPAFENTKTGIPYPRVNLKTGVPPDTNNETCT 306

Query: 214 SGCGSLILEMGALSRLTGDPRFESAALRALRKLWSMRSS-LNLLGTTLDVETGEWIEFSS 273
           +G GSL++E G LSRL GD  FE  A RA++ LW++RS+   LLG  ++++TG W+   S
Sbjct: 307 AGAGSLLVEFGILSRLLGDSTFEWVARRAVKALWNLRSNDTGLLGNVVNIQTGHWVGKQS 366

Query: 274 GIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAYLAVQKYFRHG-----------PWYHEA 333
           G+GAG+DSFYEYL K+++LFG++E   MF+ AY ++Q Y R G           P Y   
Sbjct: 367 GLGAGLDSFYEYLLKSYILFGEKEDLEMFNAAYQSIQNYLRRGREACNEGEGDPPLYVNV 426

Query: 334 DMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWEKYGVLPERHAASKSIY 393
           +M +G+     + SLQAF+PGLQVL+GD+  A   H  ++ +W++YG LPER+       
Sbjct: 427 NMFSGQLMNTWIDSLQAFFPGLQVLIGDVEDAICLHAFYYAIWKRYGALPERY------- 486

Query: 394 ISYPVYLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLVLYTKVE 453
                   + Q+  P   +YPLRPEL EST+ LYQATK+P+Y  VG  I++SL  YTKV+
Sbjct: 487 --------NWQLQAPDVLFYPLRPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKVK 546

Query: 454 GGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQN---YIFTTEGHPLPVLS 513
            G+A++  V     ED   SFFLSETCKYLYLLFD+   V ++   Y+FTTEGH + V  
Sbjct: 547 CGYATLHHVIDKSTEDRMESFFLSETCKYLYLLFDEDNPVHKSGTRYMFTTEGHIVSVDE 606

Query: 514 SWHERLPEVYGLANGTSIKGENSSRRLSAMSLQVCPATS 533
              E   + +    G   +G  S  R     L+V  ++S
Sbjct: 607 HLRELPWKEFFSEEGGQDQGGKSVHRPKPHELKVINSSS 627

BLAST of MS001943 vs. ExPASy Swiss-Prot
Match: Q925U4 (ER degradation-enhancing alpha-mannosidase-like protein 1 OS=Mus musculus OX=10090 GN=Edem1 PE=1 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 3.4e-109
Identity = 224/519 (43.16%), Postives = 316/519 (60.89%), Query Frame = 0

Query: 34  RMRMREKVRRMFYHAYDNYMTYAFPHDELKPL-TKTFTDSLSELGNLKLEHLPQNYNGSA 93
           R +MR+  R MF   YDNYM +AFP DEL P+  +       +  NL +  +  NY   +
Sbjct: 122 RAQMRDLARGMFVFGYDNYMAHAFPQDELNPIYCRGRGPDRGDPSNLNINDVLGNY---S 181

Query: 94  LTLIESLSSLVILGNNTEFQKAVLWLSENLHFDVDARINLFECNIRVLGGLVSAHVLATD 153
           LTL+++L +L I+GN++EFQKAV  +   + FD D+ + +FE  IRVLG L+SAH + TD
Sbjct: 182 LTLVDALDTLAIMGNSSEFQKAVKLVINTVSFDKDSTVQVFEATIRVLGSLLSAHRIITD 241

Query: 154 ST---NRLARQNYKNQLLVLAEDLGNRLLPAF-NTPTGLPYAWINLKHGVMENETTETST 213
           S      +  ++Y N+LL +A DL  RLLPAF NT TG+PY  +NLK GV  +   ET T
Sbjct: 242 SKQPFGDMTIEDYDNELLYMAHDLAVRLLPAFENTKTGIPYPRVNLKTGVPPDSNNETCT 301

Query: 214 SGCGSLILEMGALSRLTGDPRFESAALRALRKLWSMRSS-LNLLGTTLDVETGEWIEFSS 273
           +G GSL++E G LSRL GD  FE  A RA++ LW++RS+   LLG  ++++TG W+   S
Sbjct: 302 AGAGSLLVEFGILSRLLGDSTFEWVARRAVKALWNLRSNDTGLLGNVVNIQTGHWVGKQS 361

Query: 274 GIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAYLAVQKYFRHG-----------PWYHEA 333
           G+GAG+DSFYEYL K+++LFG++E   MF+ AY ++Q Y R G           P Y   
Sbjct: 362 GLGAGLDSFYEYLLKSYILFGEKEDLEMFNAAYQSIQSYLRRGREACNEGEGDPPLYVNV 421

Query: 334 DMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWEKYGVLPERHAASKSIY 393
           +M +G+     + SLQAF+PGLQVL+GD+  A   H  ++ +W++YG LPER+       
Sbjct: 422 NMFSGQLMNTWIDSLQAFFPGLQVLIGDVEDAICLHAFYYAIWKRYGALPERY------- 481

Query: 394 ISYPVYLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLVLYTKVE 453
                   + Q+  P   +YPLRPEL EST+ LYQATK+P+Y  VG  I++SL  YTKV+
Sbjct: 482 --------NWQLQAPDVLFYPLRPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKVK 541

Query: 454 GGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQN---YIFTTEGHPLPVLS 513
            G+A++  V     ED   SFFLSETCKYLYLLFD+   V ++   Y+FTTEGH + V  
Sbjct: 542 CGYATLHHVIDKSKEDRMESFFLSETCKYLYLLFDEENPVHKSGTRYMFTTEGHIISVDK 601

Query: 514 SWHERLPEVYGLANGTSIKGENSSRRLSAMSLQVCPATS 533
              E   + +   +G   + E    R  +  L+V  ++S
Sbjct: 602 RLRELPWKEFFSEDGERDQEEKFVHRPKSQELRVINSSS 622

BLAST of MS001943 vs. ExPASy Swiss-Prot
Match: Q9BV94 (ER degradation-enhancing alpha-mannosidase-like protein 2 OS=Homo sapiens OX=9606 GN=EDEM2 PE=1 SV=2)

HSP 1 Score: 351.7 bits (901), Expect = 1.6e-95
Identity = 199/486 (40.95%), Postives = 277/486 (57.00%), Query Frame = 0

Query: 38  REKVRRMFYHAYDNYMTYAFPHDELKPLTKTFTDSLSELGNLKLEHLPQNYNGSALTLIE 97
           RE+V+ MFYHAYD+Y+  AFP DEL+PLT    D+               +   +LTLI+
Sbjct: 36  RERVKAMFYHAYDSYLENAFPFDELRPLTCDGHDT---------------WGSFSLTLID 95

Query: 98  SLSSLVILGNNTEFQKAVLWLSENLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRL 157
           +L +L+ILGN +EFQ+ V  L +++ FD+D   ++FE NIRV+GGL+SAH+L+  +   +
Sbjct: 96  ALDTLLILGNVSEFQRVVEVLQDSVDFDIDVNASVFETNIRVVGGLLSAHLLSKKAGVEV 155

Query: 158 -ARQNYKNQLLVLAEDLGNRLLPAFNTPTGLPYAWINLKHGVMENETTETSTSGCGSLIL 217
            A       LL +AE+   +LLPAF TPTG+PY  +NL HGV   ET  T T+G G+ I+
Sbjct: 156 EAGWPCSGPLLRMAEEAARKLLPAFQTPTGMPYGTVNLLHGVNPGETPVTCTAGIGTFIV 215

Query: 218 EMGALSRLTGDPRFESAALRALRKLWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSF 277
           E   LS LTGDP FE  A  AL +LW  RS + L+G  +DV TG+W+   +GIGAGVDS+
Sbjct: 216 EFATLSSLTGDPVFEDVARVALMRLWESRSDIGLVGNHIDVLTGKWVAQDAGIGAGVDSY 275

Query: 278 YEYLFKAHVLFGKEEFWRMFHTAYLAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWP 337
           +EYL K  +L   ++   MF     A++ Y R   WY    M  G  +     SL+A+WP
Sbjct: 276 FEYLVKGAILLQDKKLMAMFLEYNKAIRNYTRFDDWYLWVQMYKGTVSMPVFQSLEAYWP 335

Query: 338 GLQVLVGDIPAANSSHREFFYVWEKYGVLPERHAASKSIYISYPVYLLDHQMLHPTEKYY 397
           GLQ L+GDI  A  +   ++ VW+++G LPE              Y +         + Y
Sbjct: 336 GLQSLIGDIDNAMRTFLNYYTVWKQFGGLPE-------------FYNIPQGYTVEKREGY 395

Query: 398 PLRPELAESTFYLYQATKDPWYFEVGESIMKSLVLYTKVEGGFASVRDVTTMQLEDHQHS 457
           PLRPEL ES  YLY+AT DP   E+G   ++S+   +KVE GFA+++D+   +L++   S
Sbjct: 396 PLRPELIESAMYLYRATGDPTLLELGRDAVESIEKISKVECGFATIKDLRDHKLDNRMES 455

Query: 458 FFLSETCKYLYLLFDDSFLVDQN--------------------YIFTTEGHPL-PVLSSW 502
           FFL+ET KYLYLLFD +  +  N                    YIF TE HP+ P     
Sbjct: 456 FFLAETVKYLYLLFDPTNFIHNNGSTFDAVITPYGECILGAGGYIFNTEAHPIDPAALHC 493

BLAST of MS001943 vs. ExPASy TrEMBL
Match: A0A6J1C218 (alpha-1,2-Mannosidase OS=Momordica charantia OX=3673 GN=LOC111007230 PE=3 SV=1)

HSP 1 Score: 1174.8 bits (3038), Expect = 0.0e+00
Identity = 573/588 (97.45%), Postives = 575/588 (97.79%), Query Frame = 0

Query: 1   MLPCRSATWILLLFFIFSALFGPSTSDDYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60
           MLPCRSATWILLLFFIFSALFGPSTSD+YWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD
Sbjct: 1   MLPCRSATWILLLFFIFSALFGPSTSDNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60

Query: 61  ELKPLTKTFTDSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120
           ELKPLTKTFTDSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE
Sbjct: 61  ELKPLTKTFTDSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120

Query: 121 NLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQNYKNQLLVLAEDLGNRLLPA 180
           NLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQNYKNQLLVLAEDLGNRLLPA
Sbjct: 121 NLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQNYKNQLLVLAEDLGNRLLPA 180

Query: 181 FNTPTGLPYAWINLKHGVMENETTETSTSGCGSLILEMGALSRLTGDPRFESAALRALRK 240
           FNTPTGLPYAWINLKHGVMENETTETSTSGCGSLILEMGALSRLTGDPRFESAALRALRK
Sbjct: 181 FNTPTGLPYAWINLKHGVMENETTETSTSGCGSLILEMGALSRLTGDPRFESAALRALRK 240

Query: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY 300
           LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY
Sbjct: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY 300

Query: 301 LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE 360
           LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE
Sbjct: 301 LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE 360

Query: 361 KYGVLPERHAASKSIYISYPVYLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE 420
           KYGVLPER             YLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE
Sbjct: 361 KYGVLPER-------------YLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE 420

Query: 421 VGESIMKSLVLYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY 480
           VGESIMKSLVLYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY
Sbjct: 421 VGESIMKSLVLYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY 480

Query: 481 IFTTEGHPLPVLSSWHERLPEVYGLANGTSIKGENSSRRLSAMSLQVCPATSLNTAGDGG 540
           IFTTEGHPLPVLSSWHERLPEVYGLANGTSIKGENSSRRLSAMSLQVCPA+SLNTAGDGG
Sbjct: 481 IFTTEGHPLPVLSSWHERLPEVYGLANGTSIKGENSSRRLSAMSLQVCPASSLNTAGDGG 540

Query: 541 QPIESACHILDARADHRCFSDEECGVDSTTCRRRSCSTSGYCGLWLHI 589
           QPIESACHILDARADHRCFSDEECGVDSTTCRRRSCSTSGYCGLWLHI
Sbjct: 541 QPIESACHILDARADHRCFSDEECGVDSTTCRRRSCSTSGYCGLWLHI 575

BLAST of MS001943 vs. ExPASy TrEMBL
Match: A0A6J1E1J2 (alpha-1,2-Mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111429983 PE=3 SV=1)

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 546/588 (92.86%), Postives = 561/588 (95.41%), Query Frame = 0

Query: 1   MLPCRSATWILLLFFIFSALFGPSTSDDYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60
           MLP RS TWILLLF IFSALF PS+S++YWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD
Sbjct: 1   MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60

Query: 61  ELKPLTKTFTDSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120
           ELKPLTKTFT+SLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE
Sbjct: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120

Query: 121 NLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQNYKNQLLVLAEDLGNRLLPA 180
           NL FDVDARINLFECNIRVLGGLVSAHVLATDST RL RQ+YKNQLLVLAEDLGNR LPA
Sbjct: 121 NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTTRLPRQSYKNQLLVLAEDLGNRFLPA 180

Query: 181 FNTPTGLPYAWINLKHGVMENETTETSTSGCGSLILEMGALSRLTGDPRFESAALRALRK 240
           FNTPTGLPYAWINLKHGVMENETTETS+SGCGSLILEMGALSRLTGDPRFESAALRALRK
Sbjct: 181 FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSRLTGDPRFESAALRALRK 240

Query: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY 300
           LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFH+AY
Sbjct: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHSAY 300

Query: 301 LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE 360
           LAVQKYFRHGPWYHEADMRTGRAT+WQLTSLQAFWPGLQVLVGDIPAANSSHREFF+VWE
Sbjct: 301 LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE 360

Query: 361 KYGVLPERHAASKSIYISYPVYLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE 420
           KYGVLPER             YLLD+QMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE
Sbjct: 361 KYGVLPER-------------YLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE 420

Query: 421 VGESIMKSLVLYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY 480
           VGESIMKSL+LYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY
Sbjct: 421 VGESIMKSLILYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY 480

Query: 481 IFTTEGHPLPVLSSWHERLPEVYGLANGTSIKGENSSRRLSAMSLQVCPATSLNTAGDGG 540
           IFTTEGHPLPVLSSWHERLPEVYGL NGTSIKGENSSRR SAMSLQVCPATSLNTAGDG 
Sbjct: 481 IFTTEGHPLPVLSSWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGD 540

Query: 541 QPIESACHILDARADHRCFSDEECGVDSTTCRRRSCSTSGYCGLWLHI 589
           Q +ESACHILDARADH+CFSDEECGVDSTTCRRRSCS+SGYCG WL +
Sbjct: 541 QQLESACHILDARADHKCFSDEECGVDSTTCRRRSCSSSGYCGHWLRL 575

BLAST of MS001943 vs. ExPASy TrEMBL
Match: A0A6J1JLB4 (alpha-1,2-Mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111485626 PE=3 SV=1)

HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 545/588 (92.69%), Postives = 562/588 (95.58%), Query Frame = 0

Query: 1   MLPCRSATWILLLFFIFSALFGPSTSDDYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60
           MLP RS TWILLLF IFSALF PS+S++YWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD
Sbjct: 1   MLPRRSFTWILLLFIIFSALFNPSSSNNYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60

Query: 61  ELKPLTKTFTDSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120
           ELKPLTKTFT+SLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE
Sbjct: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120

Query: 121 NLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQNYKNQLLVLAEDLGNRLLPA 180
           NL FDVDARINLFECNIRVLGGLVSAHVLATDSTNRL R++YKNQLLVLAEDLGNR LPA
Sbjct: 121 NLRFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLPRESYKNQLLVLAEDLGNRFLPA 180

Query: 181 FNTPTGLPYAWINLKHGVMENETTETSTSGCGSLILEMGALSRLTGDPRFESAALRALRK 240
           FNTPTGLPYAWINLKHGVMENETTETS+SGCGSLILEMGALS+LTGDPRFESAALRALRK
Sbjct: 181 FNTPTGLPYAWINLKHGVMENETTETSSSGCGSLILEMGALSQLTGDPRFESAALRALRK 240

Query: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY 300
           LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY
Sbjct: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY 300

Query: 301 LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE 360
           LAVQKYFRHGPWYHEADMRTGRAT+WQLTSLQAFWPGLQVLVGDIPAANSSHREFF+VWE
Sbjct: 301 LAVQKYFRHGPWYHEADMRTGRATHWQLTSLQAFWPGLQVLVGDIPAANSSHREFFHVWE 360

Query: 361 KYGVLPERHAASKSIYISYPVYLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE 420
           KYGVLPER             YLLD+QMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE
Sbjct: 361 KYGVLPER-------------YLLDYQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE 420

Query: 421 VGESIMKSLVLYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY 480
           VGESIMKSL+LYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY
Sbjct: 421 VGESIMKSLILYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY 480

Query: 481 IFTTEGHPLPVLSSWHERLPEVYGLANGTSIKGENSSRRLSAMSLQVCPATSLNTAGDGG 540
           IFTTEGHPLPVLSSWHERLPEVYGL NGTSIKGENSSRR SAMSLQVCPATSLNTAGDG 
Sbjct: 481 IFTTEGHPLPVLSSWHERLPEVYGLTNGTSIKGENSSRRSSAMSLQVCPATSLNTAGDGD 540

Query: 541 QPIESACHILDARADHRCFSDEECGVDSTTCRRRSCSTSGYCGLWLHI 589
           Q +ESACHILDARADH+CFS+EECGVDSTTCRRRSCS+SGYCG WL +
Sbjct: 541 QQLESACHILDARADHKCFSNEECGVDSTTCRRRSCSSSGYCGHWLRL 575

BLAST of MS001943 vs. ExPASy TrEMBL
Match: A0A1S3BWL9 (alpha-1,2-Mannosidase OS=Cucumis melo OX=3656 GN=LOC103493935 PE=3 SV=1)

HSP 1 Score: 1108.2 bits (2865), Expect = 0.0e+00
Identity = 540/588 (91.84%), Postives = 554/588 (94.22%), Query Frame = 0

Query: 1   MLPCRSATWILLLFFIFSALFGPSTSDDYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60
           ML  RS  WILLLF I   LF PS+S  YWT+KRMRMREK RRMFYHAYDNYMTYAFP+D
Sbjct: 1   MLSRRSFPWILLLFIILFTLFNPSSSSSYWTSKRMRMREKARRMFYHAYDNYMTYAFPND 60

Query: 61  ELKPLTKTFTDSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120
           ELKPLTKTFT+SLSELGNLKLEHLPQNY GSALTLIESLSSLVILGNNTEFQKAVLWLSE
Sbjct: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYTGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120

Query: 121 NLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQNYKNQLLVLAEDLGNRLLPA 180
           NLHFDVDARINLFECNIRVLGGL+SAHVLATDSTNRLA+Q+YKNQLLVLAEDLGNR LPA
Sbjct: 121 NLHFDVDARINLFECNIRVLGGLISAHVLATDSTNRLAKQSYKNQLLVLAEDLGNRFLPA 180

Query: 181 FNTPTGLPYAWINLKHGVMENETTETSTSGCGSLILEMGALSRLTGDPRFESAALRALRK 240
           FNTPTGLPYAWINLKHGV+E+ETTETSTSGCGSLILEMGALSRLTGDPRFE AALRALRK
Sbjct: 181 FNTPTGLPYAWINLKHGVVEDETTETSTSGCGSLILEMGALSRLTGDPRFEYAALRALRK 240

Query: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY 300
           LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYL KAHVLFGKEEFWRMFHTAY
Sbjct: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLLKAHVLFGKEEFWRMFHTAY 300

Query: 301 LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE 360
           LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE
Sbjct: 301 LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE 360

Query: 361 KYGVLPERHAASKSIYISYPVYLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE 420
           KYGVLPER             YLLD QMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE
Sbjct: 361 KYGVLPER-------------YLLDRQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE 420

Query: 421 VGESIMKSLVLYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY 480
           VGESIMKSL+ +TKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY
Sbjct: 421 VGESIMKSLISHTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY 480

Query: 481 IFTTEGHPLPVLSSWHERLPEVYGLANGTSIKGENSSRRLSAMSLQVCPATSLNTAGDGG 540
           IFTTEGHPLPVLSSWHERLPEVYGL NGTSIKGENSSRRLSAMSLQVCPATSLNTAGDGG
Sbjct: 481 IFTTEGHPLPVLSSWHERLPEVYGLTNGTSIKGENSSRRLSAMSLQVCPATSLNTAGDGG 540

Query: 541 QPIESACHILDARADHRCFSDEECGVDSTTCRRRSCSTSGYCGLWLHI 589
           Q IESACHILDARADH+CFSDEECGVDSTTCRRRSCS+SGYCG WLH+
Sbjct: 541 QQIESACHILDARADHKCFSDEECGVDSTTCRRRSCSSSGYCGQWLHL 575

BLAST of MS001943 vs. ExPASy TrEMBL
Match: A0A0A0LNZ0 (alpha-1,2-Mannosidase OS=Cucumis sativus OX=3659 GN=Csa_1G005710 PE=3 SV=1)

HSP 1 Score: 1105.9 bits (2859), Expect = 0.0e+00
Identity = 541/588 (92.01%), Postives = 553/588 (94.05%), Query Frame = 0

Query: 1   MLPCRSATWILLLFFIFSALFGPSTSDDYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHD 60
           ML  RS  WILLLF IF  LF PS+S  Y T+KRMRMREK RRMFYHAYDNYMTYAFP+D
Sbjct: 1   MLSRRSFAWILLLFIIFFTLFNPSSSSSYLTSKRMRMREKARRMFYHAYDNYMTYAFPND 60

Query: 61  ELKPLTKTFTDSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120
           ELKPLTKTFT+SLSELGNLKLEHLPQNY GSALTLIESLSSLVILGNNTEFQKAVLWLSE
Sbjct: 61  ELKPLTKTFTNSLSELGNLKLEHLPQNYTGSALTLIESLSSLVILGNNTEFQKAVLWLSE 120

Query: 121 NLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQNYKNQLLVLAEDLGNRLLPA 180
           NLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQ+YKNQLLVLAEDLGNR LPA
Sbjct: 121 NLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQSYKNQLLVLAEDLGNRFLPA 180

Query: 181 FNTPTGLPYAWINLKHGVMENETTETSTSGCGSLILEMGALSRLTGDPRFESAALRALRK 240
           FNTPTGLPYAWINLKHGVME+ETTETSTSGCGSLILEMGALSRLTGDPRFE AALRALRK
Sbjct: 181 FNTPTGLPYAWINLKHGVMEDETTETSTSGCGSLILEMGALSRLTGDPRFEHAALRALRK 240

Query: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAY 300
           LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYL KAHVLFGKEEFWRMFH AY
Sbjct: 241 LWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLLKAHVLFGKEEFWRMFHAAY 300

Query: 301 LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE 360
           LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE
Sbjct: 301 LAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWE 360

Query: 361 KYGVLPERHAASKSIYISYPVYLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE 420
           KYGVLPER             YLLD QMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE
Sbjct: 361 KYGVLPER-------------YLLDRQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFE 420

Query: 421 VGESIMKSLVLYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY 480
           VGESI+KSL+ +TKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY
Sbjct: 421 VGESIIKSLISHTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNY 480

Query: 481 IFTTEGHPLPVLSSWHERLPEVYGLANGTSIKGENSSRRLSAMSLQVCPATSLNTAGDGG 540
           IFTTEGHPLPVLSSWHERLPEVYGL NGTSIKGENSSRRLSAMSLQVCPATSLNTAGDGG
Sbjct: 481 IFTTEGHPLPVLSSWHERLPEVYGLTNGTSIKGENSSRRLSAMSLQVCPATSLNTAGDGG 540

Query: 541 QPIESACHILDARADHRCFSDEECGVDSTTCRRRSCSTSGYCGLWLHI 589
           Q IESACHILDARADH+CFSDEECGVDSTTCRRRSCS+SGYCG WLH+
Sbjct: 541 QQIESACHILDARADHKCFSDEECGVDSTTCRRRSCSSSGYCGQWLHL 575

BLAST of MS001943 vs. TAIR 10
Match: AT1G27520.1 (Glycosyl hydrolase family 47 protein )

HSP 1 Score: 895.2 bits (2312), Expect = 2.8e-260
Identity = 434/576 (75.35%), Postives = 487/576 (84.55%), Query Frame = 0

Query: 10  ILLLFFIFSALFGPSTSDDYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHDELKPLTKTF 69
           + L   I    F    S  +   K+ +MREKVR MFYHAYDNYMTYAFPHDELKPLTK+F
Sbjct: 10  LFLCLLISLTFFVVDPSSQHIEVKKKQMREKVREMFYHAYDNYMTYAFPHDELKPLTKSF 69

Query: 70  TDSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILGNNTEFQKAVLWLSENLHFDVDAR 129
           TDSLSELGNLKLEHLP +YNGSA+TL+ESLSSL ILGN+TEF+K VLWLSENL FD+DAR
Sbjct: 70  TDSLSELGNLKLEHLPTDYNGSAVTLVESLSSLAILGNSTEFEKGVLWLSENLTFDIDAR 129

Query: 130 INLFECNIRVLGGLVSAHVLATDSTNRLARQNYKNQLLVLAEDLGNRLLPAFNTPTGLPY 189
           +NLFECNIRVLGGL+SAH+LA D  NRL + +Y NQLL LAEDLG R LPAF TPTGLPY
Sbjct: 130 VNLFECNIRVLGGLISAHLLAIDPNNRLIQGSYNNQLLRLAEDLGKRFLPAFETPTGLPY 189

Query: 190 AWINLKHGVMENETTETSTSGCGSLILEMGALSRLTGDPRFESAALRALRKLWSMRSSLN 249
           AWINLK+GVMENETTETSTSGCGSL+LEMGALSRLTGDPRFESAALRALR+LW MRSSL+
Sbjct: 190 AWINLKNGVMENETTETSTSGCGSLVLEMGALSRLTGDPRFESAALRALRQLWRMRSSLD 249

Query: 250 LLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFKAHVLFGKEEFWRMFHTAYLAVQKYFRH 309
           LLGTTLDV TGEWIE+SS IGAGVDSFYEYL KA++LFGKE++WRMFH+AYLA QKYFRH
Sbjct: 250 LLGTTLDVVTGEWIEYSSSIGAGVDSFYEYLLKAYILFGKEDYWRMFHSAYLASQKYFRH 309

Query: 310 GPWYHEADMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWEKYGVLPERH 369
           GPWYHEA+M +G+ TYWQLTSLQAFWPGLQVLVGDI AANSSHREFF+VWEK+GVLPER 
Sbjct: 310 GPWYHEANMWSGKPTYWQLTSLQAFWPGLQVLVGDIAAANSSHREFFHVWEKFGVLPER- 369

Query: 370 AASKSIYISYPVYLLDHQMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSL 429
                       YLLDHQ++HPT KYYPLRPELAESTFYLYQATKDPWY +VGES++KSL
Sbjct: 370 ------------YLLDHQIIHPTMKYYPLRPELAESTFYLYQATKDPWYLDVGESMVKSL 429

Query: 430 VLYTKVEGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVDQNYIFTTEGHPL 489
            LYTKV GGFASVRDVTTMQLEDHQHSFFL+ETCKYLYLLFDDSF+  +NYIFTTEGHP+
Sbjct: 430 NLYTKVPGGFASVRDVTTMQLEDHQHSFFLAETCKYLYLLFDDSFVAKRNYIFTTEGHPI 489

Query: 490 PVLSSWHERLPEVYGLANGTSIKGENSSRRLSAMSLQVCPATSLNTAGDGGQPIESACHI 549
            V+SSWHE+LPE Y   N T  K      R SA+SLQVCP  SLN+     Q  ESACH+
Sbjct: 490 QVVSSWHEKLPETYFSGNWTLSKSGAWESRASALSLQVCPLISLNSR-HPEQQRESACHV 549

Query: 550 LDARADHRCFSDEECGVDSTTCRRRSCSTSGYCGLW 586
           LD + +H+C+S++ECGVD+TTCR R+CS  GYCGLW
Sbjct: 550 LDEQINHKCWSNKECGVDATTCRLRTCSGVGYCGLW 571

BLAST of MS001943 vs. TAIR 10
Match: AT5G43710.1 (Glycosyl hydrolase family 47 protein )

HSP 1 Score: 406.8 bits (1044), Expect = 3.0e-113
Identity = 213/463 (46.00%), Postives = 294/463 (63.50%), Query Frame = 0

Query: 36  RMREKVRRMFYHAYDNYMTYAFPHDELKPLTKTFTDSLSELGNLKLEHLPQNYNGSALTL 95
           ++R++VR MFYHA+D YM  AFP DEL+PL+    D+L                G ALTL
Sbjct: 39  QLRDEVRGMFYHAFDGYMNNAFPLDELRPLSCQGEDTL---------------GGYALTL 98

Query: 96  IESLSSLVILGNNTEFQKAVLWLSENLHFDVDARINLFECNIRVLGGLVSAHVLATDSTN 155
           I+SL +L +LG+   F  +V W+ +NL F+++  +++FE  IRVLGGL+SAH++A+D   
Sbjct: 99  IDSLDTLALLGDRERFTSSVEWIGKNLQFNINKTVSVFETTIRVLGGLLSAHLIASDYAT 158

Query: 156 RLARQNYKNQLLVLAEDLGNRLLPAFNTPTGLPYAWINLKHGVMENETTETSTSGCGSLI 215
            +   +Y N+LLVLAE+L  R+LPAF+TPTG+P+  +NL +GV ++E+  TST+G G+L 
Sbjct: 159 GMRIPSYNNELLVLAENLARRMLPAFDTPTGIPFGSVNLMYGVDKHESKITSTAGGGTLS 218

Query: 216 LEMGALSRLTGDPRFESAALRALRKLWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDS 275
           LE G LSRLT DP FE  A  A+R LW+ RS+L+L+G  ++V TGEW +  +GIG  +DS
Sbjct: 219 LEFGVLSRLTNDPVFEQVAKNAVRGLWARRSNLDLVGAHINVFTGEWTQKDAGIGTSIDS 278

Query: 276 FYEYLFKAHVLFGKEEFWRMFHTAYLAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFW 335
           FYEYL KA++LFG EE+  +F  AY +  +Y    PWY E +M +    +    SLQAFW
Sbjct: 279 FYEYLLKAYILFGDEEYLYIFQEAYRSAMQYLHKDPWYVEVNMDSAAIVWPVFNSLQAFW 338

Query: 336 PGLQVLVGDIPAANSSHREFFYVWEKYGVLPERHAASKSIYISYPVYLLDHQMLHPTEKY 395
           PGLQVL GD+  A  +H  FF VW++YG  PE    + ++ + Y             +K 
Sbjct: 339 PGLQVLAGDVDPAIRTHTAFFSVWKRYGFTPEGFNLA-TLSVQY------------GQKS 398

Query: 396 YPLRPELAESTFYLYQATKDPWYFEVGESIMKSLVLYTKVEGGFASVRDVTTMQLEDHQH 455
           YPLRPEL EST++LY+AT+DP Y + G   + SL    K   G+  + DV   + EDH  
Sbjct: 399 YPLRPELIESTYWLYKATRDPRYLDAGRDFVASLQYGAKCPCGYCHITDVELHKQEDHME 458

Query: 456 SFFLSETCKYLYLLF----DDSFLVDQ---NYIFTTEGHPLPV 492
           SFFL+ET KYL+LLF    D   LVD     YIF+TEGH LP+
Sbjct: 459 SFFLAETVKYLWLLFDLAVDSDNLVDNGPYKYIFSTEGHLLPI 473

BLAST of MS001943 vs. TAIR 10
Match: AT1G51590.1 (alpha-mannosidase 1 )

HSP 1 Score: 204.1 bits (518), Expect = 3.0e-52
Identity = 144/477 (30.19%), Postives = 232/477 (48.64%), Query Frame = 0

Query: 38  REKVRRMFYHAYDNYMTYAFPHDELKPLTKTFTDSLSELGNLKLEHLPQNYNGSALTLIE 97
           R+KV+    HA+ +Y  YA+  DEL+P TK  TDS   LG                T+++
Sbjct: 98  RQKVKEAMIHAWSSYEKYAWGKDELQPRTKDGTDSFGGLG---------------ATMVD 157

Query: 98  SLSSLVILGNNTEFQKAVLWLSENLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRL 157
           SL +L I+G + +FQKA  W++ +L FD D   ++FE  IRV+GGL+SA+ L+ D     
Sbjct: 158 SLDTLYIMGLDEQFQKAREWVASSLDFDKDYDASMFETTIRVVGGLLSAYDLSGD----- 217

Query: 158 ARQNYKNQLLVLAEDLGNRLLPAFNTPTGLPYAWINLKHGVMENET----TETSTSGCGS 217
                    L  A+D+ +RLLPA+NTPTG+PY  INL++G   N +     ++  +  G+
Sbjct: 218 ------KMFLEKAKDIADRLLPAWNTPTGIPYNIINLRNGNAHNPSWAAGGDSILADSGT 277

Query: 218 LILEMGALSRLTGDPRFESAALRALRKLWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGV 277
             LE  ALS+ TGDP+++    + + +L     +  LL   ++ +       ++  GA  
Sbjct: 278 EQLEFIALSQRTGDPKYQQKVEKVITELNKNFPADGLLPIYINPDNANPSYSTTTFGAMG 337

Query: 278 DSFYEYLFKAHVLFGK-------EEFWRMFHTAYLAVQKYFRHGPWYHEADMRTGRATYW 337
           DSFYEYL K  V   K        + W       L++ K      + +  + + G     
Sbjct: 338 DSFYEYLLKVWVQGNKTSAVKPYRDMWEKSMKGLLSLVKKSTPSSFTYICE-KNGNNLID 397

Query: 338 QLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWEKYGVLPERHAASKSIYISYPVYLLDH 397
           ++  L  F PG+  L       +   +      E        + ++ +       +    
Sbjct: 398 KMDELACFAPGMLALGASGYGPDEEKKFLSLAGELAWTCYNFYQSTPTKLAGENYFFTAG 457

Query: 398 QMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLVLYTKVEGGFASVRDVT 457
           Q +     +  LRPE  ES FYL++ T +  Y E G +I ++    ++VE G+  ++DV 
Sbjct: 458 QDMSVGTSWNILRPETVESLFYLWRLTGNKTYQEWGWNIFQAFEKNSRVESGYVGLKDVN 517

Query: 458 TMQLEDHQHSFFLSETCKYLYLLFDDSFLVD-QNYIFTTEGHPLPVLSSWHERLPEV 503
           T   ++   SFFL+ET KYLYLLF  S ++    ++F TE HPL +++    R P +
Sbjct: 518 TGAKDNKMQSFFLAETLKYLYLLFSPSSVISLDEWVFNTEAHPLKIVARNDPRKPTI 547

BLAST of MS001943 vs. TAIR 10
Match: AT1G51590.2 (alpha-mannosidase 1 )

HSP 1 Score: 198.4 bits (503), Expect = 1.6e-50
Identity = 141/468 (30.13%), Postives = 227/468 (48.50%), Query Frame = 0

Query: 47  HAYDNYMTYAFPHDELKPLTKTFTDSLSELGNLKLEHLPQNYNGSALTLIESLSSLVILG 106
           HA+ +Y  YA+  DEL+P TK  TDS   LG                T+++SL +L I+G
Sbjct: 3   HAWSSYEKYAWGKDELQPRTKDGTDSFGGLG---------------ATMVDSLDTLYIMG 62

Query: 107 NNTEFQKAVLWLSENLHFDVDARINLFECNIRVLGGLVSAHVLATDSTNRLARQNYKNQL 166
            + +FQKA  W++ +L FD D   ++FE  IRV+GGL+SA+ L+ D              
Sbjct: 63  LDEQFQKAREWVASSLDFDKDYDASMFETTIRVVGGLLSAYDLSGD-----------KMF 122

Query: 167 LVLAEDLGNRLLPAFNTPTGLPYAWINLKHGVMENET----TETSTSGCGSLILEMGALS 226
           L  A+D+ +RLLPA+NTPTG+PY  INL++G   N +     ++  +  G+  LE  ALS
Sbjct: 123 LEKAKDIADRLLPAWNTPTGIPYNIINLRNGNAHNPSWAAGGDSILADSGTEQLEFIALS 182

Query: 227 RLTGDPRFESAALRALRKLWSMRSSLNLLGTTLDVETGEWIEFSSGIGAGVDSFYEYLFK 286
           + TGDP+++    + + +L     +  LL   ++ +       ++  GA  DSFYEYL K
Sbjct: 183 QRTGDPKYQQKVEKVITELNKNFPADGLLPIYINPDNANPSYSTTTFGAMGDSFYEYLLK 242

Query: 287 AHVLFGK-------EEFWRMFHTAYLAVQKYFRHGPWYHEADMRTGRATYWQLTSLQAFW 346
             V   K        + W       L++ K      + +  + + G     ++  L  F 
Sbjct: 243 VWVQGNKTSAVKPYRDMWEKSMKGLLSLVKKSTPSSFTYICE-KNGNNLIDKMDELACFA 302

Query: 347 PGLQVLVGDIPAANSSHREFFYVWEKYGVLPERHAASKSIYISYPVYLLDHQMLHPTEKY 406
           PG+  L       +   +      E        + ++ +       +    Q +     +
Sbjct: 303 PGMLALGASGYGPDEEKKFLSLAGELAWTCYNFYQSTPTKLAGENYFFTAGQDMSVGTSW 362

Query: 407 YPLRPELAESTFYLYQATKDPWYFEVGESIMKSLVLYTKVEGGFASVRDVTTMQLEDHQH 466
             LRPE  ES FYL++ T +  Y E G +I ++    ++VE G+  ++DV T   ++   
Sbjct: 363 NILRPETVESLFYLWRLTGNKTYQEWGWNIFQAFEKNSRVESGYVGLKDVNTGAKDNKMQ 422

Query: 467 SFFLSETCKYLYLLFDDSFLVD-QNYIFTTEGHPLPVLSSWHERLPEV 503
           SFFL+ET KYLYLLF  S ++    ++F TE HPL +++    R P +
Sbjct: 423 SFFLAETLKYLYLLFSPSSVISLDEWVFNTEAHPLKIVARNDPRKPTI 443

BLAST of MS001943 vs. TAIR 10
Match: AT3G21160.1 (alpha-mannosidase 2 )

HSP 1 Score: 196.4 bits (498), Expect = 6.3e-50
Identity = 145/479 (30.27%), Postives = 236/479 (49.27%), Query Frame = 0

Query: 27  DDYWTAKRMRMREKVRRMFYHAYDNYMTYAFPHDELKPLTKTFTDSLSELGNLKLEHLPQ 86
           +D   A+RM   ++V+    HA+ +Y  YA+  DEL+P TK   DS   LG         
Sbjct: 91  EDPVDAQRM---QRVKEAMVHAWSSYEKYAWGQDELQPQTKDGVDSFGGLG--------- 150

Query: 87  NYNGSALTLIESLSSLVILGNNTEFQKAVLWLSENLHFDVDARINLFECNIRVLGGLVSA 146
                  T+I++L +L I+G + +FQKA  W++ +L FD D   ++FE  IRV+GGL+SA
Sbjct: 151 ------ATMIDALDTLYIMGLDEQFQKAREWVASSLDFDKDYAASMFETTIRVVGGLLSA 210

Query: 147 HVLATDSTNRLARQNYKNQLLVLAEDLGNRLLPAFNTPTGLPYAWINLKHGVMENET--- 206
           + L+ D              L  A D+ +RLLPA++T +G+PY  INLKHG   N T   
Sbjct: 211 YDLSGDKI-----------FLEKAMDIADRLLPAWDTQSGIPYNIINLKHGNAHNPTWAG 270

Query: 207 TETSTSGCGSLILEMGALSRLTGDPRFESAALRALRKLWSMRSSLNLLGTTLDVETGEWI 266
            ++  +  G+  LE  ALS+ TGDP+++    + +  L     +  LL   ++ +T    
Sbjct: 271 GDSILADSGTEQLEFIALSQRTGDPKYQQKVEKVISVLNKNFPADGLLPIYINPDTANPS 330

Query: 267 EFSSGIGAGVDSFYEYLFKAHVLFGK-------EEFWRMFHTAYLAVQKYFRHGPWYHEA 326
           + +   GA  DSFYEYL K  V   K        + W       L++ K      + +  
Sbjct: 331 QSTITFGAMGDSFYEYLLKVWVFGNKTSAVKHYRDMWEKSMNGLLSLVKKSTPLSFTYIC 390

Query: 327 DMRTGRATYWQLTSLQAFWPGLQVLVGDIPAANSSHREFFYVWEKYGVLPERHAASKSIY 386
           + ++G +   ++  L  F PG+  L     +  +  ++F  + E+          S    
Sbjct: 391 E-KSGNSLIDKMDELACFAPGMLALGASGYSDPAEGKKFLTLAEELAWTCYNFYQSTPTK 450

Query: 387 ISYPVYLLDH-QMLHPTEKYYPLRPELAESTFYLYQATKDPWYFEVGESIMKSLVLYTKV 446
           ++   Y  +    +     +  LRPE  ES FYL++ T +  Y E G +I ++    +++
Sbjct: 451 LAGENYFFNSGSDMSVGTSWNILRPETVESLFYLWRLTGNKTYQEWGWNIFEAFEKNSRI 510

Query: 447 EGGFASVRDVTTMQLEDHQHSFFLSETCKYLYLLFDDSFLVD-QNYIFTTEGHPLPVLS 494
           E G+  ++DV T   ++   SFFL+ET KYLYLLF  + ++    ++F TE HPL + S
Sbjct: 511 ESGYVGLKDVNTGVKDNKMQSFFLAETLKYLYLLFSPTTVIPLDEWVFNTEAHPLKIKS 539