MS001888 (gene) Bitter gourd (TR) v1

Overview
NameMS001888
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionferric reduction oxidase 8, mitochondrial
Locationscaffold30: 692759 .. 695516 (-)
RNA-Seq ExpressionMS001888
SyntenyMS001888
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTCTATTATGTGCTGCTTGGGTTTCTCTTTGGCTCTTAAAACCCACCGATCTCTGGACAAGAAAATGGCGTCTCGCTGAAAACAGTGCCCGAGCCACTGTATTTGGATACTATGGTACAGAATCGACATGTTGTTCAGATTCATCTTTCTTGCATTTGCAGATGCATGCATTTACTGATTATTATTATTATACAAGTGAAGTAAGTTTCATACTTTTCAGGTCTCAATTTCGTTGTGTACACTTTTCCTGTTATTGCTCTTGTTATAATTGGGCTGGTTTACTTGAATTTCAAACCCTCCAAGCCACGAACTTTCAGGCAAGTTCATCTGGGTCGCAGTCCATTTGAACAATGAAATTTCTATACGCTTATAAACTTGGCCTCTTGATCCTCGTATTAAATCTTTTTGCATTACAGGCGATCAAAGTTCTCAACTTTTTCCAATGCCCTCTCGAACCCACTTGTTGTGAACAGTTATCTTGGTGTTTTATCTGGAACTGAGATTCTTGGTGTAGCTTTTTTTCTCCTCTTCCTGGTTTGGACATTCTATGCTCGAATTTCTAAAGATTTCAGAAAGTTGATGCCCGCCGAATCACTCAACTTGCAGATGTGAGTGCTTTTGAAGAACAAATAAGAATGCCCTTTCTTTGTTTTCTGATCTGGGGTTGAATTATTATCAATTTTGATGATTCAGCTGATTTTCAGATGGGAACTGAAGTATCTAAGAGTAGCCACCAGGTTCGGTCTGCTGGCAGAGGCCTGTTTGGGGCTGCTTCTTCTGCCTATTTTGAGAGGATTGGCTCCATTTCGGCTGATTGGCATTCAGTTTGAAGCTTCAGTGAAGTATCACATCTGGCTTGGCACTGCAATGGTGTTATTTGCCACCTTTCATGGCGCAAGTACCTTGTTCATTTGGGGGCTCAGCAACCAGATTCAAGATCAGGTAACTGTTCTTCTTTGCCTCTCTGTTGCTAATTATGTACTCCATTGACAATGTCAATCATTCCATTGGATTACTGTTGTGTTGAAACTAGATGTGGAAATGGCAGAACACCGGCCGGATTTACATCGCCGGAGAGATTACTTTGATTACAGGACTGGTGATTTGGATCACTTCCCTCCCTCAGATAAGGAGGAGAAAGTTTGAGATCTTCTACTACACTCACCATTTGTACATTGTCTTTTTTGTGTTCTTCTTGTTTCATGCTGGAGATCGACACTTCTACATGGTATTTCCCGGATTGTTTCTCTTCGGCATCGATAAGGTGCTCCGAATTATTCAATCTAGAACCGAAACTTGTGTTGTATCCGCTCAAATCCTCCCCTGCAAAGCTGTGGAGCTAACTCTGCCAAAAGATCAAAGTACTTCTTCAACTCATCCCTTTTTTTCAACTTATGGAACAAAAAGATAGTAGTTCTCAGAAAAAGCCTACTTTCTTTGCAATTGCAGGGCTGAAGTATAAGCCAACTAGTGTTGTGTATGTGAAGATACCAAGGATTTCCAGATTTCAGTGGCATGCTTTCAGCTTGATTTCCAGCTCGAGCGTCAATGATGACACAATGTCGATTGTTGTTAAATGCGATAAGTCGTGGACGAGATCTTTGTATGACATGATAAGTGCTGAACGGGAGGGAGAGTCTAATCAGTTGAAGTGCATTTCCATTGCAGTTGAAGGCCCTTATGGACCTGCCTCCATGGACTTCCTAAGGTCTACTTACGAAAACATGTTTCGGTTCGAATGTACTTGATTGATGTTTTCGATACGCATTCGAAATTGTGCATAATCCAAACACAAAATTGATCTCTTGCAGGTATGATCACTTGCTCCTTATTGCAGGAGGAATTGGAGTAACCCCTCTTTTGAGCATTTTACAGGAGATCACTTCTATGCAAAAGAGCAGTAACTTCAAATGCCCTGCAAGAATACAACTTATTCATGTCATGAAAAAGTCCCACGACGTTTCACTACTCACCTCGATCTTACCTTTACTATTGAACCGTTCGGATGCGAAAATGTATTTGCAGCTCAAAGTATTTGTGACACAAGAAGAGGAATCTAGCACAACTGTATCAGAACTGCTAAACAAGTTCTCTCAAGTGCAGACCATACAATCAAGCACGAAATGTTCGAGCCATGCAGCACAAGGGCTTGAAAGCTTGGGCGGGATGGCTACAATTACTGGATTGACATCTATCATGTTCTTCGTCGCTCTAGTCTTCTTCAACCACATCGTCATTCCCACCGAGAAGGCCTCCAAAAAGACGAAAGATCGAACTCCATCTTGGATCTCAGATCTACTCCTGATGTCTTCTTTCGCTTTAGCTATCTTATGTGGGGCGGTCGTGGGGATCATTTTAAGATGGAGAAAACTGAAGAAAGAAACTGTAGCAGTCATCCAAAGCCAAAACCAAAATGCAGTTCCAAGTTCAGAAGCAAGAGTGAGTATACAGGAAGAACATGAAATACAGTTTGGAGGAAGGCCCAACTTCAAAGGTATGGTATCTCTTGTACAAACTCATTATAATTTTGATATTCAAAGCAAGATATGGTCGTAACTCGTCGAGGATTGAACAGATATACTCTCCGACATCCAAAATGATGCGGGTGGGTCAGAAATCGGCGTGATCAGCTGCGGACCGGAGACGATGAACATGTCTGTAGCATCAGCATGCAAGGAAATGTGTGAATGTTTAAGGAGAGGCTCAAAGAAGAAGAAACAGAAATTCAATTTCCACTCCCTCAACTTCACACTC

mRNA sequence

ATGATTCTATTATGTGCTGCTTGGGTTTCTCTTTGGCTCTTAAAACCCACCGATCTCTGGACAAGAAAATGGCGTCTCGCTGAAAACAGTGCCCGAGCCACTGTATTTGGATACTATGGTCTCAATTTCGTTGTGTACACTTTTCCTGTTATTGCTCTTGTTATAATTGGGCTGGTTTACTTGAATTTCAAACCCTCCAAGCCACGAACTTTCAGGCAATTCTCAACTTTTTCCAATGCCCTCTCGAACCCACTTGTTGTGAACAGTTATCTTGGTGTTTTATCTGGAACTGAGATTCTTGGTGTAGCTTTTTTTCTCCTCTTCCTGGTTTGGACATTCTATGCTCGAATTTCTAAAGATTTCAGAAAGTTGATGCCCGCCGAATCACTCAACTTGCAGATATGGGAACTGAAGTATCTAAGAGTAGCCACCAGGTTCGGTCTGCTGGCAGAGGCCTGTTTGGGGCTGCTTCTTCTGCCTATTTTGAGAGGATTGGCTCCATTTCGGCTGATTGGCATTCAGTTTGAAGCTTCAGTGAAGTATCACATCTGGCTTGGCACTGCAATGGTGTTATTTGCCACCTTTCATGGCGCAAGTACCTTGTTCATTTGGGGGCTCAGCAACCAGATTCAAGATCAGGTAACTGTTCTTCTTTGCCTCTCTGTTGCTAATTATATGTGGAAATGGCAGAACACCGGCCGGATTTACATCGCCGGAGAGATTACTTTGATTACAGGACTGGTGATTTGGATCACTTCCCTCCCTCAGATAAGGAGGAGAAAGTTTGAGATCTTCTACTACACTCACCATTTGTACATTGTCTTTTTTGTGTTCTTCTTGTTTCATGCTGGAGATCGACACTTCTACATGGTATTTCCCGGATTGTTTCTCTTCGGCATCGATAAGGTGCTCCGAATTATTCAATCTAGAACCGAAACTTGTGTTGTATCCGCTCAAATCCTCCCCTGCAAAGCTGTGGAGCTAACTCTGCCAAAAGATCAAAGGCTGAAGTATAAGCCAACTAGTGTTGTGTATGTGAAGATACCAAGGATTTCCAGATTTCAGTGGCATGCTTTCAGCTTGATTTCCAGCTCGAGCGTCAATGATGACACAATGTCGATTGTTGTTAAATGCGATAAGTCGTGGACGAGATCTTTGTATGACATGATAAGTGCTGAACGGGAGGGAGAGTCTAATCAGTTGAAGTGCATTTCCATTGCAGTTGAAGGCCCTTATGGACCTGCCTCCATGGACTTCCTAAGGTATGATCACTTGCTCCTTATTGCAGGAGGAATTGGAGTAACCCCTCTTTTGAGCATTTTACAGGAGATCACTTCTATGCAAAAGAGCAGTAACTTCAAATGCCCTGCAAGAATACAACTTATTCATGTCATGAAAAAGTCCCACGACGTTTCACTACTCACCTCGATCTTACCTTTACTATTGAACCGTTCGGATGCGAAAATGTATTTGCAGCTCAAAGTATTTGTGACACAAGAAGAGGAATCTAGCACAACTGTATCAGAACTGCTAAACAAGTTCTCTCAAGTGCAGACCATACAATCAAGCACGAAATGTTCGAGCCATGCAGCACAAGGGCTTGAAAGCTTGGGCGGGATGGCTACAATTACTGGATTGACATCTATCATGTTCTTCGTCGCTCTAGTCTTCTTCAACCACATCGTCATTCCCACCGAGAAGGCCTCCAAAAAGACGAAAGATCGAACTCCATCTTGGATCTCAGATCTACTCCTGATGTCTTCTTTCGCTTTAGCTATCTTATGTGGGGCGGTCGTGGGGATCATTTTAAGATGGAGAAAACTGAAGAAAGAAACTGTAGCAGTCATCCAAAGCCAAAACCAAAATGCAGTTCCAAGTTCAGAAGCAAGAGTGAGTATACAGGAAGAACATGAAATACAGTTTGGAGGAAGGCCCAACTTCAAAGATATACTCTCCGACATCCAAAATGATGCGGGTGGGTCAGAAATCGGCGTGATCAGCTGCGGACCGGAGACGATGAACATGTCTGTAGCATCAGCATGCAAGGAAATGTGTGAATGTTTAAGGAGAGGCTCAAAGAAGAAGAAACAGAAATTCAATTTCCACTCCCTCAACTTCACACTC

Coding sequence (CDS)

ATGATTCTATTATGTGCTGCTTGGGTTTCTCTTTGGCTCTTAAAACCCACCGATCTCTGGACAAGAAAATGGCGTCTCGCTGAAAACAGTGCCCGAGCCACTGTATTTGGATACTATGGTCTCAATTTCGTTGTGTACACTTTTCCTGTTATTGCTCTTGTTATAATTGGGCTGGTTTACTTGAATTTCAAACCCTCCAAGCCACGAACTTTCAGGCAATTCTCAACTTTTTCCAATGCCCTCTCGAACCCACTTGTTGTGAACAGTTATCTTGGTGTTTTATCTGGAACTGAGATTCTTGGTGTAGCTTTTTTTCTCCTCTTCCTGGTTTGGACATTCTATGCTCGAATTTCTAAAGATTTCAGAAAGTTGATGCCCGCCGAATCACTCAACTTGCAGATATGGGAACTGAAGTATCTAAGAGTAGCCACCAGGTTCGGTCTGCTGGCAGAGGCCTGTTTGGGGCTGCTTCTTCTGCCTATTTTGAGAGGATTGGCTCCATTTCGGCTGATTGGCATTCAGTTTGAAGCTTCAGTGAAGTATCACATCTGGCTTGGCACTGCAATGGTGTTATTTGCCACCTTTCATGGCGCAAGTACCTTGTTCATTTGGGGGCTCAGCAACCAGATTCAAGATCAGGTAACTGTTCTTCTTTGCCTCTCTGTTGCTAATTATATGTGGAAATGGCAGAACACCGGCCGGATTTACATCGCCGGAGAGATTACTTTGATTACAGGACTGGTGATTTGGATCACTTCCCTCCCTCAGATAAGGAGGAGAAAGTTTGAGATCTTCTACTACACTCACCATTTGTACATTGTCTTTTTTGTGTTCTTCTTGTTTCATGCTGGAGATCGACACTTCTACATGGTATTTCCCGGATTGTTTCTCTTCGGCATCGATAAGGTGCTCCGAATTATTCAATCTAGAACCGAAACTTGTGTTGTATCCGCTCAAATCCTCCCCTGCAAAGCTGTGGAGCTAACTCTGCCAAAAGATCAAAGGCTGAAGTATAAGCCAACTAGTGTTGTGTATGTGAAGATACCAAGGATTTCCAGATTTCAGTGGCATGCTTTCAGCTTGATTTCCAGCTCGAGCGTCAATGATGACACAATGTCGATTGTTGTTAAATGCGATAAGTCGTGGACGAGATCTTTGTATGACATGATAAGTGCTGAACGGGAGGGAGAGTCTAATCAGTTGAAGTGCATTTCCATTGCAGTTGAAGGCCCTTATGGACCTGCCTCCATGGACTTCCTAAGGTATGATCACTTGCTCCTTATTGCAGGAGGAATTGGAGTAACCCCTCTTTTGAGCATTTTACAGGAGATCACTTCTATGCAAAAGAGCAGTAACTTCAAATGCCCTGCAAGAATACAACTTATTCATGTCATGAAAAAGTCCCACGACGTTTCACTACTCACCTCGATCTTACCTTTACTATTGAACCGTTCGGATGCGAAAATGTATTTGCAGCTCAAAGTATTTGTGACACAAGAAGAGGAATCTAGCACAACTGTATCAGAACTGCTAAACAAGTTCTCTCAAGTGCAGACCATACAATCAAGCACGAAATGTTCGAGCCATGCAGCACAAGGGCTTGAAAGCTTGGGCGGGATGGCTACAATTACTGGATTGACATCTATCATGTTCTTCGTCGCTCTAGTCTTCTTCAACCACATCGTCATTCCCACCGAGAAGGCCTCCAAAAAGACGAAAGATCGAACTCCATCTTGGATCTCAGATCTACTCCTGATGTCTTCTTTCGCTTTAGCTATCTTATGTGGGGCGGTCGTGGGGATCATTTTAAGATGGAGAAAACTGAAGAAAGAAACTGTAGCAGTCATCCAAAGCCAAAACCAAAATGCAGTTCCAAGTTCAGAAGCAAGAGTGAGTATACAGGAAGAACATGAAATACAGTTTGGAGGAAGGCCCAACTTCAAAGATATACTCTCCGACATCCAAAATGATGCGGGTGGGTCAGAAATCGGCGTGATCAGCTGCGGACCGGAGACGATGAACATGTCTGTAGCATCAGCATGCAAGGAAATGTGTGAATGTTTAAGGAGAGGCTCAAAGAAGAAGAAACAGAAATTCAATTTCCACTCCCTCAACTTCACACTC

Protein sequence

MILLCAAWVSLWLLKPTDLWTRKWRLAENSARATVFGYYGLNFVVYTFPVIALVIIGLVYLNFKPSKPRTFRQFSTFSNALSNPLVVNSYLGVLSGTEILGVAFFLLFLVWTFYARISKDFRKLMPAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAPFRLIGIQFEASVKYHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQVTVLLCLSVANYMWKWQNTGRIYIAGEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLFGIDKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVKIPRISRFQWHAFSLISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIAVEGPYGPASMDFLRYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKKSHDVSLLTSILPLLLNRSDAKMYLQLKVFVTQEEESSTTVSELLNKFSQVQTIQSSTKCSSHAAQGLESLGGMATITGLTSIMFFVALVFFNHIVIPTEKASKKTKDRTPSWISDLLLMSSFALAILCGAVVGIILRWRKLKKETVAVIQSQNQNAVPSSEARVSIQEEHEIQFGGRPNFKDILSDIQNDAGGSEIGVISCGPETMNMSVASACKEMCECLRRGSKKKKQKFNFHSLNFTL
Homology
BLAST of MS001888 vs. NCBI nr
Match: XP_022135163.1 (ferric reduction oxidase 8, mitochondrial [Momordica charantia])

HSP 1 Score: 1330.1 bits (3441), Expect = 0.0e+00
Identity = 690/710 (97.18%), Postives = 692/710 (97.46%), Query Frame = 0

Query: 1   MILLCAAWVSLWLLKPTDLWTRKWRLAENSARATVFGYYGLNFVVYTFPVIALVIIGLVY 60
           MILLCAAWVSLWLLKPTDLWTRKWRLAENSARATVFGYYGLNFVVYTFPVIALVIIGLVY
Sbjct: 19  MILLCAAWVSLWLLKPTDLWTRKWRLAENSARATVFGYYGLNFVVYTFPVIALVIIGLVY 78

Query: 61  LNFKPSKPRTFR--QFSTFSNALSNPLVVNSYLGVLSGTEILGVAFFLLFLVWTFYARIS 120
           LNFKPSKPRTFR  +FSTFSNALSNPLVVNSYLGVLSGTEILGVAFFLLFLVWTFYARIS
Sbjct: 79  LNFKPSKPRTFRRSKFSTFSNALSNPLVVNSYLGVLSGTEILGVAFFLLFLVWTFYARIS 138

Query: 121 KDFRKLMPAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAPFRLIGIQFEAS 180
           KDFRKLMPAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAPFRLIGIQFEAS
Sbjct: 139 KDFRKLMPAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAPFRLIGIQFEAS 198

Query: 181 VKYHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQVTVLLCLSVANYMWKWQNTGRIYIA 240
           VKYHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQ            MWKWQNTGRIYIA
Sbjct: 199 VKYHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQ------------MWKWQNTGRIYIA 258

Query: 241 GEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF 300
           GEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF
Sbjct: 259 GEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF 318

Query: 301 GIDKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVKIPRISRFQWHA 360
           GIDKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVKIPRISRFQWHA
Sbjct: 319 GIDKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVKIPRISRFQWHA 378

Query: 361 FSLISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIAVEGPYGPASMD 420
           FSLISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIAVEGPYGPASMD
Sbjct: 379 FSLISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIAVEGPYGPASMD 438

Query: 421 FLRYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKKSHDVSLLTSIL 480
           FLRYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKKSHDVSLLTSIL
Sbjct: 439 FLRYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKKSHDVSLLTSIL 498

Query: 481 PLLLNRSDAKMYLQLKVFVTQEEESSTTVSELLNKFSQVQTIQSSTKCSSHAAQGLESLG 540
           PLLLN  DAKMYLQLKVFVTQEEESSTTVSELLNKFSQVQTIQSSTKCSSHAAQGLESLG
Sbjct: 499 PLLLNHLDAKMYLQLKVFVTQEEESSTTVSELLNKFSQVQTIQSSTKCSSHAAQGLESLG 558

Query: 541 GMATITGLTSIMFFVALVFFNHIVIPTEKASKKTKDRTPSWISDLLLMSSFALAILCGAV 600
           GMATITGLTSIMFFVALVFFNHIVIPTEKASKKTKDRTPSWISDLLLMSSFALAILCGAV
Sbjct: 559 GMATITGLTSIMFFVALVFFNHIVIPTEKASKKTKDRTPSWISDLLLMSSFALAILCGAV 618

Query: 601 VGIILRWRKLKKETVAVIQSQNQNAVPSSEARVSIQEEHEIQFGGRPNFKDILSDIQNDA 660
           VGIILRWR+LKKETVAVIQSQNQNAVPSSEAR SIQEEHEIQFGGRPNFKDI SDIQNDA
Sbjct: 619 VGIILRWRRLKKETVAVIQSQNQNAVPSSEARASIQEEHEIQFGGRPNFKDIFSDIQNDA 678

Query: 661 GGSEIGVISCGPETMNMSVASACKEMCECLRRGSKKKKQKFNFHSLNFTL 709
           GGSEIGVISCGPETMNMSVASACKEMCECLRRGSKKKKQKFNFHSLNFTL
Sbjct: 679 GGSEIGVISCGPETMNMSVASACKEMCECLRRGSKKKKQKFNFHSLNFTL 716

BLAST of MS001888 vs. NCBI nr
Match: XP_008459876.1 (PREDICTED: ferric reduction oxidase 8, mitochondrial [Cucumis melo] >XP_008459877.1 PREDICTED: ferric reduction oxidase 8, mitochondrial [Cucumis melo])

HSP 1 Score: 1146.0 bits (2963), Expect = 0.0e+00
Identity = 587/712 (82.44%), Postives = 641/712 (90.03%), Query Frame = 0

Query: 1   MILLCAAWVSLWLLKPTDLWTRKWRLAENSARATVFGYYGLNFVVYTFPVIALVIIGLVY 60
           MILLCA W+SLWLLKPTDLWT+KW LAENSARA+ FGYYGLNFVVYTFPVI LVIIGLVY
Sbjct: 19  MILLCAGWISLWLLKPTDLWTKKWHLAENSARASPFGYYGLNFVVYTFPVIVLVIIGLVY 78

Query: 61  LNFKPSKPRTFRQ--FSTFSNALSNPLVVNSYLGVLSGTEILGVAFFLLFLVWTFYARIS 120
           LN KPS P+TFRQ  FSTFSNALSNPLVVN+YLG+LSG E+LG+A FLLFL WTFYARIS
Sbjct: 79  LNNKPSNPQTFRQTKFSTFSNALSNPLVVNNYLGILSGIELLGIALFLLFLAWTFYARIS 138

Query: 121 KDFRKLMPAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAPFRLIGIQFEAS 180
           KDFRKL+P ESLNL+IWELKYLR+ATRFGLLAEACLGLLLLPILRGLAPF+LIGIQFEAS
Sbjct: 139 KDFRKLLPVESLNLKIWELKYLRIATRFGLLAEACLGLLLLPILRGLAPFQLIGIQFEAS 198

Query: 181 VKYHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQVTVLLCLSVANYMWKWQNTGRIYIA 240
           VKYH+WLGTAMVLF TFHG STLFIWG+SN IQDQV            WKWQNTGRIYIA
Sbjct: 199 VKYHVWLGTAMVLFGTFHGISTLFIWGISNNIQDQV------------WKWQNTGRIYIA 258

Query: 241 GEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF 300
           GEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF
Sbjct: 259 GEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF 318

Query: 301 GIDKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVKIPRISRFQWHA 360
           GIDK+LRIIQSR ETCVVSA+ILPCKAVELTLPKD+RLKYKPTSVVYVKIPRISRFQWHA
Sbjct: 319 GIDKILRIIQSRIETCVVSARILPCKAVELTLPKDRRLKYKPTSVVYVKIPRISRFQWHA 378

Query: 361 FSLISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIAVEGPYGPASMD 420
           FSLISSS+V+D+TMSIVVKCDKSWTRSLYD+IS+EREGES+QLKCISIAVEGPYGP+SMD
Sbjct: 379 FSLISSSNVDDETMSIVVKCDKSWTRSLYDVISSEREGESDQLKCISIAVEGPYGPSSMD 438

Query: 421 FLRYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKKSHDVSLLTSIL 480
           FLRYDHLLLIAGGIG+TP LSILQEI SMQ SS FKCPARIQLIHVMK S D+SLL SIL
Sbjct: 439 FLRYDHLLLIAGGIGITPFLSILQEINSMQNSSRFKCPARIQLIHVMKNSQDISLLNSIL 498

Query: 481 PLLLNRSDAKMYLQLKVFVTQEEESSTTVSELLNKFSQVQTIQSSTKCSSHAAQGLESLG 540
           PLLLN S+ KM LQLKVFVTQE +S  TV ELLNKFSQ+QTIQSSTKCSS+AAQGLES  
Sbjct: 499 PLLLNHSNKKMNLQLKVFVTQEVQSGATVVELLNKFSQMQTIQSSTKCSSYAAQGLESFN 558

Query: 541 GMATITGLTSIMFFVALVFFNHIVIPTEKASKKTKDRTPSWISDLLLMSSFALAILCGAV 600
            MA ITGLTSI+F + L+ FNHIVIPT+ ASKKTKDRTPSWISDLLLMSSF LAIL GAV
Sbjct: 559 WMAAITGLTSIVFLITLIIFNHIVIPTKTASKKTKDRTPSWISDLLLMSSFTLAILSGAV 618

Query: 601 VGIILRWRKLKKETVAVIQSQ--NQNAVPSSEARVSIQEEHEIQFGGRPNFKDILSDIQN 660
           VGI+LRWR+LKKE +A+IQ++   QNA+PSS  R S QEEHEI +GGRPNFK+I S+I++
Sbjct: 619 VGIVLRWRRLKKENLALIQTKTGTQNAIPSSVVRGSRQEEHEIYYGGRPNFKEIFSNIKH 678

Query: 661 DAGGSEIGVISCGPETMNMSVASACKEMCECLRRGSKKKKQKFNFHSLNFTL 709
           +  G+EIGVISCGPE MN+SVAS CK++ + LR+ SKKKK +F FHSLNFTL
Sbjct: 679 EDEGTEIGVISCGPEAMNVSVASTCKQISQGLRKDSKKKKPQFIFHSLNFTL 718

BLAST of MS001888 vs. NCBI nr
Match: XP_011656798.1 (ferric reduction oxidase 8, mitochondrial [Cucumis sativus])

HSP 1 Score: 1139.8 bits (2947), Expect = 0.0e+00
Identity = 585/712 (82.16%), Postives = 643/712 (90.31%), Query Frame = 0

Query: 1   MILLCAAWVSLWLLKPTDLWTRKWRLAENSARATVFGYYGLNFVVYTFPVIALVIIGLVY 60
           MILLCA W+SLWLLKPTDLWT+KW LAENSARA++FGYYGLNFVVYTFPVI LV+IGLV+
Sbjct: 19  MILLCAGWISLWLLKPTDLWTKKWHLAENSARASLFGYYGLNFVVYTFPVIVLVMIGLVH 78

Query: 61  LNFKPSKPRTFRQ--FSTFSNALSNPLVVNSYLGVLSGTEILGVAFFLLFLVWTFYARIS 120
           LN KPS P+TFRQ  FSTFSNALSNPLVVN+YLG+LSG E+LGVA FLLFL WTFYARIS
Sbjct: 79  LNNKPSSPQTFRQTKFSTFSNALSNPLVVNNYLGILSGIELLGVALFLLFLAWTFYARIS 138

Query: 121 KDFRKLMPAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAPFRLIGIQFEAS 180
           KDFRKL+P ESLNL+IWELKYLR+ATRFGLLAEACL LLLLPILRGLAPF+LIGIQFEAS
Sbjct: 139 KDFRKLLPVESLNLKIWELKYLRIATRFGLLAEACLSLLLLPILRGLAPFQLIGIQFEAS 198

Query: 181 VKYHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQVTVLLCLSVANYMWKWQNTGRIYIA 240
           VKYH+WLGTAMVLF TFHG STLFIWG+SN+IQD+V            WKWQNTGRIYIA
Sbjct: 199 VKYHVWLGTAMVLFGTFHGISTLFIWGISNKIQDEV------------WKWQNTGRIYIA 258

Query: 241 GEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF 300
           GEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF
Sbjct: 259 GEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF 318

Query: 301 GIDKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVKIPRISRFQWHA 360
           GIDK+LRIIQSR ETCVVSA+ILPCKAVELTLPKD+RLKYKPTSVVYVKIPRISRFQWHA
Sbjct: 319 GIDKILRIIQSRIETCVVSARILPCKAVELTLPKDRRLKYKPTSVVYVKIPRISRFQWHA 378

Query: 361 FSLISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIAVEGPYGPASMD 420
           FSLISSSSV+D+TMSIVVKCD+SWTRSLYD+IS EREGES+QLKCISIAVEGPYGP+SMD
Sbjct: 379 FSLISSSSVDDETMSIVVKCDESWTRSLYDVISTEREGESDQLKCISIAVEGPYGPSSMD 438

Query: 421 FLRYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKKSHDVSLLTSIL 480
           FLRYDHLLLIAGGIG+TP LSILQEI SMQ SS FKCPARIQLIHVMK S D+SLL SIL
Sbjct: 439 FLRYDHLLLIAGGIGITPFLSILQEINSMQNSSRFKCPARIQLIHVMKNSQDISLLNSIL 498

Query: 481 PLLLNRSDAKMYLQLKVFVTQEEESSTTVSELLNKFSQVQTIQSSTKCSSHAAQGLESLG 540
           PLLLN S+ KM LQLKVFVTQE +S  TV ELLNKFSQ+QTIQSST+CSS+AAQGLESL 
Sbjct: 499 PLLLNSSNKKMNLQLKVFVTQEVQSGATVVELLNKFSQMQTIQSSTRCSSYAAQGLESLN 558

Query: 541 GMATITGLTSIMFFVALVFFNHIVIPTEKASKKTKDRTPSWISDLLLMSSFALAILCGAV 600
            MA ITGLTSI+F VAL+ FNHIVIPT+KASKKTKDRTPSWISDLLLMSSF LAIL  AV
Sbjct: 559 WMAAITGLTSIVFLVALIIFNHIVIPTKKASKKTKDRTPSWISDLLLMSSFTLAILSSAV 618

Query: 601 VGIILRWRKLKKETVAVIQSQ--NQNAVPSSEARVSIQEEHEIQFGGRPNFKDILSDIQN 660
           VGI+LRWR+LKKE +A+IQ++   QNA+PSS  R S QEEHEI +GGRP FK+I S+I++
Sbjct: 619 VGIVLRWRRLKKENLALIQTETGTQNAIPSSVVRGSRQEEHEIYYGGRPIFKEIFSNIKH 678

Query: 661 DAGGSEIGVISCGPETMNMSVASACKEMCECLRRGSKKKKQKFNFHSLNFTL 709
           +  G+EIGVISCGP  MN+SVASACK++ + LR+ SKKK+Q+F FHSLNFTL
Sbjct: 679 EDEGTEIGVISCGPAAMNVSVASACKQISQGLRKDSKKKRQQFIFHSLNFTL 718

BLAST of MS001888 vs. NCBI nr
Match: XP_022965033.1 (ferric reduction oxidase 8, mitochondrial [Cucurbita moschata])

HSP 1 Score: 1138.6 bits (2944), Expect = 0.0e+00
Identity = 587/712 (82.44%), Postives = 644/712 (90.45%), Query Frame = 0

Query: 1   MILLCAAWVSLWLLKPTDLWTRKWRLAENSARATVFGYYGLNFVVYTFPVIALVIIGLVY 60
           MILLCA WVSLWLLKPTDLWTRKWR+AEN+ARA+VFGYYGLNFVVYTFPV+ALVIIGLVY
Sbjct: 43  MILLCAGWVSLWLLKPTDLWTRKWRVAENTARASVFGYYGLNFVVYTFPVVALVIIGLVY 102

Query: 61  LNFKPSKPRTFRQ--FSTFSNALSNPLVVNSYLGVLSGTEILGVAFFLLFLVWTFYARIS 120
           LN K SKP+T RQ  FSTFSN LSNPLVVNSY+G+LSG E+LGVA FLLFL WTFYARIS
Sbjct: 103 LNIKHSKPQTCRQTKFSTFSNTLSNPLVVNSYVGILSGIELLGVALFLLFLAWTFYARIS 162

Query: 121 KDFRKLMPAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAPFRLIGIQFEAS 180
           KDFRKLMP ESLNL+IWELKYLRVATRFGLLAEACLGLLLLP+LRGLAPFRLIGIQFEAS
Sbjct: 163 KDFRKLMPEESLNLKIWELKYLRVATRFGLLAEACLGLLLLPVLRGLAPFRLIGIQFEAS 222

Query: 181 VKYHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQVTVLLCLSVANYMWKWQNTGRIYIA 240
           VKYH+WLGTAMVLF TFHGASTLFIWG+SNQIQDQV            WKWQNTGRIYIA
Sbjct: 223 VKYHVWLGTAMVLFGTFHGASTLFIWGISNQIQDQV------------WKWQNTGRIYIA 282

Query: 241 GEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF 300
           GEITLITGLVIWITSLPQIRR KFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF
Sbjct: 283 GEITLITGLVIWITSLPQIRRIKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF 342

Query: 301 GIDKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVKIPRISRFQWHA 360
            IDK+LRIIQSRTETCVVSA++LPCKAVELTLPKD RLKYKPTSVVYVKIPRISRF+WHA
Sbjct: 343 AIDKILRIIQSRTETCVVSARVLPCKAVELTLPKDHRLKYKPTSVVYVKIPRISRFEWHA 402

Query: 361 FSLISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIAVEGPYGPASMD 420
           FSLISSSSV+D+TMSIVVKC+KSWT+SLY++I+ EREGES+QLKCIS+AVEGPYGPAS+D
Sbjct: 403 FSLISSSSVDDETMSIVVKCNKSWTKSLYEIINTEREGESDQLKCISVAVEGPYGPASLD 462

Query: 421 FLRYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKKSHDVSLLTSIL 480
           FLRYDHL+LIAGGIGVTPLLSILQEITSM+ ++ F CPARIQLIHVMKKSH+++LL+SIL
Sbjct: 463 FLRYDHLILIAGGIGVTPLLSILQEITSMKNNTKFICPARIQLIHVMKKSHEIALLSSIL 522

Query: 481 PLLLNRSDAKMYLQLKVFVTQEEESSTTVSELLNKFSQVQTIQSSTKCSSHAAQGLESLG 540
           PLLL R D KM+LQLKVFVTQE +SS+T++ELLNKFSQ+QTIQSS  CSS+AA GLESLG
Sbjct: 523 PLLLKRLDGKMHLQLKVFVTQELQSSSTLTELLNKFSQMQTIQSSVNCSSYAAHGLESLG 582

Query: 541 GMATITGLTSIMFFVALVFFNHIVIPTEKASKKTKDRTPSWISDLLLMSSFALAILCGAV 600
            MA ITGL SI+FFVAL+ FNHIV+PT+KASKKTKDRTPSWISDLLLMSSFALAIL GAV
Sbjct: 583 WMAVITGLISIVFFVALIVFNHIVVPTKKASKKTKDRTPSWISDLLLMSSFALAILSGAV 642

Query: 601 VGIILRWRKLKKETVAVIQSQNQ--NAVPSSEARVSIQEEHEIQFGGRPNFKDILSDIQN 660
           VGI+LR R+LKK  +A+IQSQNQ  N  PSS AR S QEEHEI FG RPNF +I S+I N
Sbjct: 643 VGIVLRRRRLKKGNLALIQSQNQTKNTTPSSVARGSRQEEHEIYFGERPNFIEIFSNINN 702

Query: 661 DAGGSEIGVISCGPETMNMSVASACKEMCECLRRGSKKKKQKFNFHSLNFTL 709
            AG S+IGVIS GPE MN+SVA ACK+M    R+GSKK KQ+F+FHSLNFTL
Sbjct: 703 PAGESDIGVISSGPEAMNLSVAKACKQMSGGSRKGSKKNKQQFSFHSLNFTL 742

BLAST of MS001888 vs. NCBI nr
Match: XP_022987228.1 (ferric reduction oxidase 8, mitochondrial [Cucurbita maxima])

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 585/712 (82.16%), Postives = 643/712 (90.31%), Query Frame = 0

Query: 1   MILLCAAWVSLWLLKPTDLWTRKWRLAENSARATVFGYYGLNFVVYTFPVIALVIIGLVY 60
           MILLCA WVSLWLLKPTDLWTRKWR+AEN+ARA+VFGYYGLNFVVYTFPVIALVIIGLVY
Sbjct: 19  MILLCAGWVSLWLLKPTDLWTRKWRVAENTARASVFGYYGLNFVVYTFPVIALVIIGLVY 78

Query: 61  LNFKPSKPRTFRQ--FSTFSNALSNPLVVNSYLGVLSGTEILGVAFFLLFLVWTFYARIS 120
           LN KPSKP+T RQ  FSTFSN LSNPLVVNSY+G+LSG E+LGVA FLLFL WTFYARIS
Sbjct: 79  LNIKPSKPQTCRQTKFSTFSNTLSNPLVVNSYVGILSGIELLGVALFLLFLAWTFYARIS 138

Query: 121 KDFRKLMPAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAPFRLIGIQFEAS 180
           KDFRKLMP ESLNL+IWELKYLRVATRFGLLAEACLGLLLLP+LRGLAPFRLIGIQFEAS
Sbjct: 139 KDFRKLMPEESLNLKIWELKYLRVATRFGLLAEACLGLLLLPVLRGLAPFRLIGIQFEAS 198

Query: 181 VKYHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQVTVLLCLSVANYMWKWQNTGRIYIA 240
           VKYH+WLGTAMVLF T HGASTLFIWG+SNQIQDQV            WKWQNTGRIYIA
Sbjct: 199 VKYHVWLGTAMVLFGTIHGASTLFIWGISNQIQDQV------------WKWQNTGRIYIA 258

Query: 241 GEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF 300
           GEITLITGLVIWITSLPQIRR KFEIFYYTHHLYIVFFVFFLFHAGDRHFY++FPGLFLF
Sbjct: 259 GEITLITGLVIWITSLPQIRRIKFEIFYYTHHLYIVFFVFFLFHAGDRHFYIIFPGLFLF 318

Query: 301 GIDKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVKIPRISRFQWHA 360
            IDK+LRIIQSRTETCVVSA++LPCKAVELTLPKDQRLKYKPTSVVYVKIPRISRF+WHA
Sbjct: 319 AIDKILRIIQSRTETCVVSARVLPCKAVELTLPKDQRLKYKPTSVVYVKIPRISRFEWHA 378

Query: 361 FSLISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIAVEGPYGPASMD 420
           FSLISSSSV+D+T+SIVVKC+KSWT+SLY++I+ EREGES+QLKCIS+AVEGPYGPAS+D
Sbjct: 379 FSLISSSSVDDETISIVVKCNKSWTKSLYEIINTEREGESDQLKCISVAVEGPYGPASLD 438

Query: 421 FLRYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKKSHDVSLLTSIL 480
           FLRYDHL+LIAGGIGVTPLLSILQEITSM+ ++ F CPARIQLIHVMKKSH+V+LL+SIL
Sbjct: 439 FLRYDHLILIAGGIGVTPLLSILQEITSMKNNTKFICPARIQLIHVMKKSHEVALLSSIL 498

Query: 481 PLLLNRSDAKMYLQLKVFVTQEEESSTTVSELLNKFSQVQTIQSSTKCSSHAAQGLESLG 540
           PLLL R D KM+LQLKVFVTQE +S +T++ELLNKFSQ+QTI SS   SS+AA GLESLG
Sbjct: 499 PLLLKRLDGKMHLQLKVFVTQELQSGSTLTELLNKFSQMQTIHSSVNRSSYAAHGLESLG 558

Query: 541 GMATITGLTSIMFFVALVFFNHIVIPTEKASKKTKDRTPSWISDLLLMSSFALAILCGAV 600
            MA ITGL SI+FFVAL+ FNHIV+PTEKASKKTKDRTPSWISDLLLMSSFALAIL GAV
Sbjct: 559 WMAVITGLISIVFFVALIVFNHIVVPTEKASKKTKDRTPSWISDLLLMSSFALAILSGAV 618

Query: 601 VGIILRWRKLKKETVAVIQSQNQ--NAVPSSEARVSIQEEHEIQFGGRPNFKDILSDIQN 660
           VGI+LRWR+LKK  +A+IQSQNQ  N  PSS AR S QEEHEI FG RPNF +I S+I N
Sbjct: 619 VGIVLRWRRLKKCNLALIQSQNQTKNTTPSSVARGSRQEEHEIHFGERPNFIEIFSNINN 678

Query: 661 DAGGSEIGVISCGPETMNMSVASACKEMCECLRRGSKKKKQKFNFHSLNFTL 709
            AG S+IGVIS GPE MN+SVA ACK+M    R+GS+K KQ+F+FHSLNFTL
Sbjct: 679 PAGESDIGVISSGPEAMNLSVAKACKQMSGGSRKGSEKNKQQFSFHSLNFTL 718

BLAST of MS001888 vs. ExPASy Swiss-Prot
Match: Q8VY13 (Ferric reduction oxidase 8, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FRO8 PE=1 SV=1)

HSP 1 Score: 667.2 bits (1720), Expect = 2.1e-190
Identity = 356/737 (48.30%), Postives = 493/737 (66.89%), Query Frame = 0

Query: 1   MILLCAAWVSLWLLKPTDLWTRKWRLAENSARATVFGYYGLNFVVYTFPVIALVIIGLVY 60
           M LL   W+SLW++KPT +W + WR AE++AR T FGYYGLNF V++FP IAL I+GL+Y
Sbjct: 14  MNLLLIGWISLWIIKPTTIWIQSWRQAEDTARHTFFGYYGLNFAVFSFPPIALSIVGLIY 73

Query: 61  LNFKPSKPRTFRQFSTFSNALSNPLVVNSYLGVLSGTEILGVAFFLLFLVWTFYARISKD 120
           L+  P      R     +  +S P ++NS++G++S  EIL +  FLLFL W FYAR+S D
Sbjct: 74  LSLLPQHHHPTRGGRGAAITVSRPAIINSFIGIVSCFEILALLLFLLFLAWNFYARVSND 133

Query: 121 FRKLMPAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAPFRLIGIQFEASVK 180
           F+KLMP +++NL +W+LKY RVATRFGLLAEACL LLL P+LRGL+ FRL+ I+F ASVK
Sbjct: 134 FKKLMPVKTMNLNLWQLKYYRVATRFGLLAEACLSLLLFPVLRGLSMFRLLNIEFAASVK 193

Query: 181 YHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQVTVLLCLSVANYMWKWQNTGRIYIAGE 240
           YH+W GT ++ F+  HG STLFIW +++ I++++            WKWQ TGR+Y+AG 
Sbjct: 194 YHVWFGTGLIFFSLVHGGSTLFIWTITHHIEEEI------------WKWQRTGRVYVAGL 253

Query: 241 ITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLFGI 300
           I+L+TGL++WITSLPQIRR+ FE+FYYTHHLYIVF V FLFHAGDRHFY V PG+FLFG+
Sbjct: 254 ISLVTGLLMWITSLPQIRRKNFEVFYYTHHLYIVFLVAFLFHAGDRHFYWVLPGMFLFGL 313

Query: 301 DKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVKIPRISRFQWHAFS 360
           DK+LRI+QSR+E+C++SA +  CKA+EL LPKD  L Y P+S +++ IP +SRFQWH FS
Sbjct: 314 DKILRIVQSRSESCILSANLFSCKAIELVLPKDPMLNYAPSSFIFLNIPLVSRFQWHPFS 373

Query: 361 LISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIAVEGPYGPASMDFL 420
           +ISSSSV+  ++SI++KC+  WT S+Y+ I  E     N++  I + VEGPYGPAS+DFL
Sbjct: 374 IISSSSVDKHSLSIMMKCEGDWTNSVYNKIE-EAANCENKINNIIVRVEGPYGPASVDFL 433

Query: 421 RYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKKSHDVSLLTSILPL 480
           RYD+L L+AGGIG+TP LSIL+E+ S    +  K P R+QL+  ++   D+++L  I  +
Sbjct: 434 RYDNLFLVAGGIGITPFLSILKELAS---KNRLKSPKRVQLVFAVRTFQDLNMLLPIASI 493

Query: 481 LLNRSDAKMYLQLKVFVTQEEESS---TTVSELLNKFSQVQTIQSST--KCSSHAAQGLE 540
           + N     + L+LKVFVTQE++ S   TT+ E L + SQVQ+I   T    S    +G E
Sbjct: 494 IFN-PIYNLNLKLKVFVTQEKKPSNGTTTLQEFLAQ-SQVQSIHLGTDEDYSRFPIRGPE 553

Query: 541 SLGGMATITGLTSIMFFVALVFFNHIVIPTEK-------------ASKKTKDRTPSWISD 600
           S   +AT+  +T + F   L+  +H  IP+E              A K  K++ PSW+ D
Sbjct: 554 SFRWLATLVLITVLTFLGFLIGLSHFFIPSEHKNHSGVMKLAASGAMKTAKEKVPSWVPD 613

Query: 601 LLLMSSFALAILCGAVVGIILRWRKLKKET-----VAVIQSQNQNAVPSSEARVSIQEEH 660
           L+++ S+ +AI  G     IL+ R+  KE        VI+ + +N        + I EEH
Sbjct: 614 LIIIVSYVIAISVGGFAATILQRRRKHKEAPRMSKEVVIKPEERNFTELKP--IPITEEH 673

Query: 661 EIQFGGRPNFKDILSDIQ-NDAGGSEIGVISCGPETMNMSVASACKEMCEC-----LRRG 709
           EI  G RP  ++I+S+ + N  G S +GV+ CGPE++  +VAS C++  +C     LRR 
Sbjct: 674 EIHIGERPKLEEIMSEFEKNLRGWSSVGVLVCGPESVKEAVASMCRQWPQCFGVEDLRR- 728

BLAST of MS001888 vs. ExPASy Swiss-Prot
Match: Q9LMM2 (Probable ferric reduction oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=FRO1 PE=2 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 1.2e-100
Identity = 218/699 (31.19%), Postives = 381/699 (54.51%), Query Frame = 0

Query: 1   MILLCAAWVSLWLLKPTDLWTRKW--RLAENSARATVFGYYGLNFVVYTFPVIALVIIGL 60
           M+++    + +W++ PT  +   W   +     ++  +G  G+N +VY FP+I L  +G 
Sbjct: 19  MMVILMGTIVIWIMMPTSTYKEIWLTSMRAKLGKSIYYGRPGVNLLVYMFPMILLAFLGC 78

Query: 61  VYLNFKPSKPRTFRQFST-------FSNALSNPLVVNSYLGVLSGTEILGVAFFLLFLVW 120
           +YL+ K  K  T  QF++          AL  P++VN  LG+++ TE++ +  F+  L+W
Sbjct: 79  IYLHLK--KSTTVNQFNSGVEKKRAKFGALRRPMLVNGPLGIVTVTEVMFLTMFMALLLW 138

Query: 121 TFYARISKDFRKLM--PAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAPFR 180
           +    + + F  +    A +    +W+ +   +A R G++   CL  L  P+ RG +   
Sbjct: 139 SLANYMYRTFVNVTSESAATDGNNLWQARLDLIAVRIGIVGNICLAFLFYPVARGSSLLA 198

Query: 181 LIGIQFEASVKYHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQVTVLLCLSVANYMWKW 240
            +G+  E+S+KYHIWLG  +++  T HG      W   NQ+             + M +W
Sbjct: 199 AVGLTSESSIKYHIWLGHLVMIIFTSHGLCYFIYWISKNQL------------VSKMLEW 258

Query: 241 QNTGRIYIAGEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFY 300
             T    +AGEI L+ GL++W+T+ P+IRRR FE+F+Y+H+LYIVF +FF+FH G  H  
Sbjct: 259 DRTAVSNLAGEIALVAGLMMWVTTYPKIRRRLFEVFFYSHYLYIVFMLFFVFHVGISHAL 318

Query: 301 MVFPGLFLFGIDKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVKIP 360
           +  PG ++F +D+ LR +QSR    +VSA++LPC  VEL   K+  L Y PTS ++V IP
Sbjct: 319 IPLPGFYIFLVDRFLRFLQSRNNVKLVSARVLPCDTVELNFSKNPMLMYSPTSTMFVNIP 378

Query: 361 RISRFQWHAFSLISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIAVE 420
            IS+ QWH F++ISSS +  +T+S+++K    W+  LYDM+S+     S+Q+  ++++VE
Sbjct: 379 SISKLQWHPFTIISSSKLEPETLSVMIKSQGKWSTKLYDMLSS---SSSDQINRLAVSVE 438

Query: 421 GPYGPASMDFLRYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKKSH 480
           GPYGP+S DFLR++ L++++GG G+TP +SI++++  M  +   K P ++ LI   K S 
Sbjct: 439 GPYGPSSTDFLRHESLVMVSGGSGITPFISIVRDLFYMSSTHKCKIP-KMTLICAFKNSS 498

Query: 481 DVSLLTSILPLLLNRSDAKMY--LQLKVFVTQEEESSTTVSELLNKFSQVQTIQSSTKCS 540
           D+S+L  ILP     +D   +  +Q+K FVT+EE+  T+V E  +  + ++T       S
Sbjct: 499 DLSMLDLILPTSGLTTDMASFVDIQIKAFVTREEK--TSVKESTHNRNIIKTRHFKPNVS 558

Query: 541 SHAAQGLESLGGMATITGLTSIMFFVALVFF----NHIVIPTEKASKKTKDRTPSW-ISD 600
                 +        +  + S  F + +V       + + P ++ S+K      +W    
Sbjct: 559 DQPISPILGPNSWLCLAAILSSSFMIFIVIIAIITRYHIHPIDQNSEKY-----TWAYKS 618

Query: 601 LLLMSSFALAILCGAVVGIILRWRKLKKETVAVIQSQNQNAVPSSEARVSIQEEHEIQFG 660
           L+ + S ++ ++  +   ++   +K   +    + + +   + SS  ++ I +  +I +G
Sbjct: 619 LIYLVSISITVVTTSTAAMLWNKKKYYAKNDQYVDNLSPVIIESSPQQL-ISQSTDIHYG 678

Query: 661 GRPNFKDILSDIQNDAGGSEIGVISCGPETMNMSVASAC 682
            RPN   +L  ++    GS +G++ CGP+ M   VA  C
Sbjct: 679 ERPNLNKLLVGLK----GSSVGILVCGPKKMRQKVAKIC 687

BLAST of MS001888 vs. ExPASy Swiss-Prot
Match: F4I4K7 (Ferric reduction oxidase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FRO3 PE=2 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 5.0e-99
Identity = 223/701 (31.81%), Postives = 387/701 (55.21%), Query Frame = 0

Query: 1   MILLCAAWVSLWLLKPTDLWTRKW--RLAENSARATVFGYYGLNFVVYTFPVIALVIIGL 60
           M++L    V +W++ PT  + + W   +     ++  FG  G+N +VY FP+I L  +G 
Sbjct: 43  MVILMGT-VVIWIMMPTSTYKKIWLKSMRAKLGKSIYFGKPGVNLLVYMFPMILLASLGS 102

Query: 61  VYLNFKPSKPRTFRQFSTFSN--------ALSNPLVVNSYLGVLSGTEILGVAFFLLFLV 120
           +YL+ K  K     QF++  +        AL  P++V + LG+++ TE++ +  F+  L+
Sbjct: 103 IYLHLK--KQTRVNQFNSRMDRKKIDKFGALKRPMLVKAGLGIVTVTEVMFLMMFMALLL 162

Query: 121 WT----FYARISKDFRKLMPAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLA 180
           W+    FY        + +P +  NL  W+ +   +A R GL    CLG L  P+ RG +
Sbjct: 163 WSLANYFYHTFVTITPQSLPTDGDNL--WQARLDSIAVRLGLTGNICLGFLFYPVARGSS 222

Query: 181 PFRLIGIQFEASVKYHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQVTVLLCLSVANYM 240
               +G+  E+S KYHIWLG  ++   T HG      W  +NQ+             + M
Sbjct: 223 LLAAVGLTSESSTKYHIWLGNLVMTLFTSHGLCYCIYWISTNQV-------------SQM 282

Query: 241 WKWQNTGRIYIAGEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDR 300
            +W  TG  ++AGEI L+ GL++W T+ P IRRR FE+F+YTH+LY+VF +FF+FH G  
Sbjct: 283 LEWDRTGISHLAGEIALVAGLLMWATTFPAIRRRFFEVFFYTHYLYMVFMLFFVFHVGIS 342

Query: 301 HFYMVFPGLFLFGIDKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYV 360
           +  + FPG ++F +D+ LR +QSR    +VSA++LPC+ VEL   K+  L Y PTS+++V
Sbjct: 343 YALISFPGFYIFMVDRFLRFLQSRNNVKLVSARVLPCETVELNFSKNPMLMYSPTSILFV 402

Query: 361 KIPRISRFQWHAFSLISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISI 420
            IP IS+ QWH F++ SSS +    +S+++K    W+  L+ M+++     SNQ+  +++
Sbjct: 403 NIPSISKLQWHPFTITSSSKLEPKKLSVMIKSQGKWSSKLHHMLAS-----SNQIDHLAV 462

Query: 421 AVEGPYGPASMDFLRYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMK 480
           +VEGPYGPAS D+LR+D L++++GG G+TP +SI++++  +  ++ +K P +I LI   K
Sbjct: 463 SVEGPYGPASTDYLRHDSLVMVSGGSGITPFISIIRDLLYVSSTNAYKTP-KITLICAFK 522

Query: 481 KSHDVSLLTSILPLLLNRSDAKMY--LQLKVFVTQEEESSTTVSELLNKFSQVQTIQSST 540
            S D+S+L  ILP   N ++   +  +Q+K FVT+E+ S+  +       + ++T+    
Sbjct: 523 NSSDLSMLNLILP---NSTEISSFIDIQIKAFVTREKVSTCNM-------NIIKTLSFKP 582

Query: 541 KCSSHAAQ---GLESLGGMATITGLTSIMFFVAL-VFFNHIVIPTEKASKKTKDRTPSWI 600
             S        G  S   +ATI   + ++F + + +   + + P +++SK+      S  
Sbjct: 583 YVSDQPISPILGPNSWLWLATILSSSFMIFIIIIAIISRYHIYPIDQSSKE----YTSAY 642

Query: 601 SDLLLMSSFALAILCGAVVGIILRWRKLKKETVAVIQSQNQNAVPSSEARVSIQEEHEIQ 660
           + L+ + + +++++  + V ++   +   K     + + +   + SS  ++ + E   I 
Sbjct: 643 TSLIYLLAISISVVATSTVAMLCNKKSYFKGLYQNVDALSPLMIESSPDQL-LPEFTNIH 700

Query: 661 FGGRPNFKDILSDIQNDAGGSEIGVISCGPETMNMSVASAC 682
           +G RPN   +L  ++    GS +GV+ CGP  M   VA  C
Sbjct: 703 YGERPNLNKLLVGLK----GSSVGVLVCGPRKMREEVAKIC 700

BLAST of MS001888 vs. ExPASy Swiss-Prot
Match: P92949 (Ferric reduction oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=FRO2 PE=1 SV=2)

HSP 1 Score: 359.4 bits (921), Expect = 9.5e-98
Identity = 222/732 (30.33%), Postives = 383/732 (52.32%), Query Frame = 0

Query: 1   MILLCAAWVSLWLLKPTDLWTRKW--RLAENSARATVFGYYGLNFVVYTFPVIALVIIGL 60
           M+++    + LW++ PT  +  KW   L      +T FG  G    +Y FP++ +  +G 
Sbjct: 31  MMVIFLGTIMLWIMMPTLTYRTKWLPHLRIKFGTSTYFGATGTTLFMYMFPMMVVACLGC 90

Query: 61  VYLNFKPSKPRTFRQFSTFS---NALSNPLVVNSYLGVLSGTEILGVAFFLLFLVWTFYA 120
           VYL+FK  K        T     + L  P++V   LG++S TEI  +A F+  L+W F  
Sbjct: 91  VYLHFKNRKSPHHIDRETKGGVWSKLRKPMLVKGPLGIVSVTEITFLAMFVALLLWCFIT 150

Query: 121 RISKDFRKLMP--AESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAPFRLIGI 180
            +   F  + P  A + +  +W+ K    A R GL+   CL  L LP+ RG +    +G+
Sbjct: 151 YLRNSFATITPKSAAAHDESLWQAKLESAALRLGLIGNICLAFLFLPVARGSSLLPAMGL 210

Query: 181 QFEASVKYHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQVTVLLCLSVANYMWKWQNTG 240
             E+S+KYHIWLG  ++   T HG   +  W   ++I   +              W   G
Sbjct: 211 TSESSIKYHIWLGHMVMALFTVHGLCYIIYWASMHEISQMI-------------MWDTKG 270

Query: 241 RIYIAGEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFP 300
              +AGEI L  GLV+W T+ P+IRRR FE+F+YTH+LYIVF +FF+ H G    ++  P
Sbjct: 271 VSNLAGEIALAAGLVMWATTYPKIRRRFFEVFFYTHYLYIVFMLFFVLHVGISFSFIALP 330

Query: 301 GLFLFGIDKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVKIPRISR 360
           G ++F +D+ LR +QSR    +++A+ILP   +ELT  K+ +L Y PTS+++V IP IS+
Sbjct: 331 GFYIFLVDRFLRFLQSRENVRLLAARILPSDTMELTFSKNSKLVYSPTSIMFVNIPSISK 390

Query: 361 FQWHAFSLISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIAVEGPYG 420
            QWH F++ SSS +  + +SIV+K +  W+  L+  +S+     S+Q+  ++++VEGPYG
Sbjct: 391 LQWHPFTITSSSKLEPEKLSIVIKKEGKWSTKLHQRLSS-----SDQIDRLAVSVEGPYG 450

Query: 421 PASMDFLRYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKKSHDVSL 480
           PAS DFLR++ L+++ GG G+TP +S+++++ +  +    K P +I LI   KKS ++S+
Sbjct: 451 PASADFLRHEALVMVCGGSGITPFISVIRDLIATSQKETCKIP-KITLICAFKKSSEISM 510

Query: 481 LTSILPL--LLNRSDAKMYLQLKVFVTQEEESSTTVSELLNKFSQVQTIQSSTKCSSHAA 540
           L  +LPL  L     + + ++++ F+T++ ++         K  +++T+      S  + 
Sbjct: 511 LDLVLPLSGLETELSSDINIKIEAFITRDNDAGDEA-----KAGKIKTLWFKPSLSDQSI 570

Query: 541 Q---GLESLGGMATITGLTSIMFFVAL-VFFNHIVIPTEKASKKTKDRTPSWISDLLLMS 600
               G  S   +  I   + ++F + + +   + + P +  + K    T   I  +L++S
Sbjct: 571 SSILGPNSWLWLGAILASSFLIFMIIIGIITRYYIYPIDHNTNKIYSLTSKTIIYILVIS 630

Query: 601 SFALAILCGAVVGIILRWRKLKKETVAVIQSQNQNAVPSSE------------ARVSIQE 660
              +A    A++    ++ K++ + V  +   +  + P+S              + S+ +
Sbjct: 631 VSIMATCSAAMLWNKKKYGKVESKQVQNVDRPSPTSSPTSSWGYNSLREIESTPQESLVQ 690

Query: 661 EHEIQFGGRPNFKDILSDIQNDAGGSEIGVISCGPETMNMSVASACKEMCECLRRGSKKK 708
              + FG RPN K +L D++    GS +GV+ CGP+ M   VA  C          S   
Sbjct: 691 RTNLHFGERPNLKKLLLDVE----GSSVGVLVCGPKKMRQKVAEIC----------SSGL 724

BLAST of MS001888 vs. ExPASy Swiss-Prot
Match: Q8W110 (Ferric reduction oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=FRO4 PE=2 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 8.9e-88
Identity = 210/708 (29.66%), Postives = 366/708 (51.69%), Query Frame = 0

Query: 1   MILLCAAWVSLWLLKPTDLWTRKWR-LAENSARATVFGYYGLNFVVYTFPVIALVIIGLV 60
           M++L   W+ +W++  T+L+  KW          T FG  G N V+ T P++ + ++  V
Sbjct: 12  MVVLFLGWILVWIMISTNLFKSKWTPKLSKYLNTTYFGPQGTNLVLLTVPMMFIAVLSCV 71

Query: 61  YLNF--KPSKPRTFRQFSTFSNALSNPLVVNSYLGVLSGTEILGVAFFLLFLVWTFYARI 120
           YL+   KP++P+  R++      +   ++V + LG+++ TE+     F+  L W+ Y  +
Sbjct: 72  YLHIQKKPTQPQ--REWK-LKRIMGRVIMVMNPLGIVTATELTFSLLFVALLAWSLYNYL 131

Query: 121 SKDFRKLMPAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAPFRLIGIQFEA 180
              +   +  +  N +IW+ K+     R G +   C   L  P+ R      L+G+  E+
Sbjct: 132 YLSYHVHLHNDD-NAKIWQAKFRAFGLRIGYVGNICWAFLFFPVTRASTILPLVGLTSES 191

Query: 181 SVKYHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQVTVLLCLSVANYMWKWQNTGRIYI 240
           S+KYHIWLG         H    L  W + N++ +              + W  T    +
Sbjct: 192 SIKYHIWLGHVSNFCFLVHTVVFLIYWAMINKLME-------------TFAWNPTYVPNL 251

Query: 241 AGEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFL 300
           AG I ++ G+ +W+TSLP  RR+KFEIF+YTHHLY ++ VF++ H GD  F M+ P +FL
Sbjct: 252 AGTIAMVIGIAMWVTSLPSFRRKKFEIFFYTHHLYGLYIVFYVIHVGDSWFCMILPNIFL 311

Query: 301 FGIDKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVKIPRISRFQWH 360
           F ID+ LR +QS   + +VSA+ILP   +ELT  K   L Y PTS++++ +P IS+ QWH
Sbjct: 312 FFIDRYLRFLQSTKRSRLVSARILPSDNLELTFSKTPGLHYTPTSILFLHVPSISKIQWH 371

Query: 361 AFSLISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIAVEGPYGPASM 420
            F++ SSS++  DT+S+V++   SWT+ LY  +       S+ +  + ++ EGPYGP S 
Sbjct: 372 PFTITSSSNLEKDTLSVVIRRQGSWTQKLYTHL-------SSSIDSLEVSTEGPYGPNSF 431

Query: 421 DFLRYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKKSHDVSLLTSI 480
           D  R++ L+L++GG G+TP +S+++E+ S  ++ + K P  + L+   K  HD++ L  I
Sbjct: 432 DVSRHNSLILVSGGSGITPFISVIRELISQSQNKSTKLP-DVLLVCSFKHYHDLAFLDLI 491

Query: 481 LPLLLNRSD-AKMYLQLKVFVTQEEESSTTVSE---LLNKFSQVQTIQSSTKCSSHAAQG 540
            PL ++ SD +++ L+++ ++T+E++   T  +   L  K+ + Q + S          G
Sbjct: 492 FPLDMSASDISRLNLRIEAYITREDKKPETTDDHRLLQTKWFKPQPLDSPIS----PVLG 551

Query: 541 LESLGGMATITGLTSIMFFVAL-VFFNHIVIPTEKASKKTKDRTPSWISDLLLMSSFALA 600
             +   +  +   + +MF + + +   + + P +  +    + +   + D+ L S  A  
Sbjct: 552 PNNFLWLGVVILSSFVMFLLLIGIVTRYYIYPVDHNTGSIYNFSYRGLWDMFLGS--ACI 611

Query: 601 ILCGAVVGIILRWRKLK--------KETVAVIQSQNQNAVPSS-----EARV------SI 660
            +  +VV +   WRK +        K  V  ++ Q   + P S     E  +      SI
Sbjct: 612 FISSSVVFL---WRKKQNKEGDKEFKNQVQSVEFQTPTSSPGSWFHGHERELESVPYQSI 671

Query: 661 QEEHEIQFGGRPNFKDILSDIQNDAGGSEIGVISCGPETMNMSVASAC 682
            +   + FG +PN K IL + +   G  ++GV+ CGP  M   VA  C
Sbjct: 672 VQATSVHFGSKPNLKKILLEAE---GSEDVGVMVCGPRKMRHEVAKIC 682

BLAST of MS001888 vs. ExPASy TrEMBL
Match: A0A6J1C416 (ferric reduction oxidase 8, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111007198 PE=4 SV=1)

HSP 1 Score: 1330.1 bits (3441), Expect = 0.0e+00
Identity = 690/710 (97.18%), Postives = 692/710 (97.46%), Query Frame = 0

Query: 1   MILLCAAWVSLWLLKPTDLWTRKWRLAENSARATVFGYYGLNFVVYTFPVIALVIIGLVY 60
           MILLCAAWVSLWLLKPTDLWTRKWRLAENSARATVFGYYGLNFVVYTFPVIALVIIGLVY
Sbjct: 19  MILLCAAWVSLWLLKPTDLWTRKWRLAENSARATVFGYYGLNFVVYTFPVIALVIIGLVY 78

Query: 61  LNFKPSKPRTFR--QFSTFSNALSNPLVVNSYLGVLSGTEILGVAFFLLFLVWTFYARIS 120
           LNFKPSKPRTFR  +FSTFSNALSNPLVVNSYLGVLSGTEILGVAFFLLFLVWTFYARIS
Sbjct: 79  LNFKPSKPRTFRRSKFSTFSNALSNPLVVNSYLGVLSGTEILGVAFFLLFLVWTFYARIS 138

Query: 121 KDFRKLMPAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAPFRLIGIQFEAS 180
           KDFRKLMPAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAPFRLIGIQFEAS
Sbjct: 139 KDFRKLMPAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAPFRLIGIQFEAS 198

Query: 181 VKYHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQVTVLLCLSVANYMWKWQNTGRIYIA 240
           VKYHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQ            MWKWQNTGRIYIA
Sbjct: 199 VKYHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQ------------MWKWQNTGRIYIA 258

Query: 241 GEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF 300
           GEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF
Sbjct: 259 GEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF 318

Query: 301 GIDKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVKIPRISRFQWHA 360
           GIDKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVKIPRISRFQWHA
Sbjct: 319 GIDKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVKIPRISRFQWHA 378

Query: 361 FSLISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIAVEGPYGPASMD 420
           FSLISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIAVEGPYGPASMD
Sbjct: 379 FSLISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIAVEGPYGPASMD 438

Query: 421 FLRYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKKSHDVSLLTSIL 480
           FLRYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKKSHDVSLLTSIL
Sbjct: 439 FLRYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKKSHDVSLLTSIL 498

Query: 481 PLLLNRSDAKMYLQLKVFVTQEEESSTTVSELLNKFSQVQTIQSSTKCSSHAAQGLESLG 540
           PLLLN  DAKMYLQLKVFVTQEEESSTTVSELLNKFSQVQTIQSSTKCSSHAAQGLESLG
Sbjct: 499 PLLLNHLDAKMYLQLKVFVTQEEESSTTVSELLNKFSQVQTIQSSTKCSSHAAQGLESLG 558

Query: 541 GMATITGLTSIMFFVALVFFNHIVIPTEKASKKTKDRTPSWISDLLLMSSFALAILCGAV 600
           GMATITGLTSIMFFVALVFFNHIVIPTEKASKKTKDRTPSWISDLLLMSSFALAILCGAV
Sbjct: 559 GMATITGLTSIMFFVALVFFNHIVIPTEKASKKTKDRTPSWISDLLLMSSFALAILCGAV 618

Query: 601 VGIILRWRKLKKETVAVIQSQNQNAVPSSEARVSIQEEHEIQFGGRPNFKDILSDIQNDA 660
           VGIILRWR+LKKETVAVIQSQNQNAVPSSEAR SIQEEHEIQFGGRPNFKDI SDIQNDA
Sbjct: 619 VGIILRWRRLKKETVAVIQSQNQNAVPSSEARASIQEEHEIQFGGRPNFKDIFSDIQNDA 678

Query: 661 GGSEIGVISCGPETMNMSVASACKEMCECLRRGSKKKKQKFNFHSLNFTL 709
           GGSEIGVISCGPETMNMSVASACKEMCECLRRGSKKKKQKFNFHSLNFTL
Sbjct: 679 GGSEIGVISCGPETMNMSVASACKEMCECLRRGSKKKKQKFNFHSLNFTL 716

BLAST of MS001888 vs. ExPASy TrEMBL
Match: A0A1S3CBN3 (ferric reduction oxidase 8, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103498863 PE=4 SV=1)

HSP 1 Score: 1146.0 bits (2963), Expect = 0.0e+00
Identity = 587/712 (82.44%), Postives = 641/712 (90.03%), Query Frame = 0

Query: 1   MILLCAAWVSLWLLKPTDLWTRKWRLAENSARATVFGYYGLNFVVYTFPVIALVIIGLVY 60
           MILLCA W+SLWLLKPTDLWT+KW LAENSARA+ FGYYGLNFVVYTFPVI LVIIGLVY
Sbjct: 19  MILLCAGWISLWLLKPTDLWTKKWHLAENSARASPFGYYGLNFVVYTFPVIVLVIIGLVY 78

Query: 61  LNFKPSKPRTFRQ--FSTFSNALSNPLVVNSYLGVLSGTEILGVAFFLLFLVWTFYARIS 120
           LN KPS P+TFRQ  FSTFSNALSNPLVVN+YLG+LSG E+LG+A FLLFL WTFYARIS
Sbjct: 79  LNNKPSNPQTFRQTKFSTFSNALSNPLVVNNYLGILSGIELLGIALFLLFLAWTFYARIS 138

Query: 121 KDFRKLMPAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAPFRLIGIQFEAS 180
           KDFRKL+P ESLNL+IWELKYLR+ATRFGLLAEACLGLLLLPILRGLAPF+LIGIQFEAS
Sbjct: 139 KDFRKLLPVESLNLKIWELKYLRIATRFGLLAEACLGLLLLPILRGLAPFQLIGIQFEAS 198

Query: 181 VKYHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQVTVLLCLSVANYMWKWQNTGRIYIA 240
           VKYH+WLGTAMVLF TFHG STLFIWG+SN IQDQV            WKWQNTGRIYIA
Sbjct: 199 VKYHVWLGTAMVLFGTFHGISTLFIWGISNNIQDQV------------WKWQNTGRIYIA 258

Query: 241 GEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF 300
           GEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF
Sbjct: 259 GEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF 318

Query: 301 GIDKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVKIPRISRFQWHA 360
           GIDK+LRIIQSR ETCVVSA+ILPCKAVELTLPKD+RLKYKPTSVVYVKIPRISRFQWHA
Sbjct: 319 GIDKILRIIQSRIETCVVSARILPCKAVELTLPKDRRLKYKPTSVVYVKIPRISRFQWHA 378

Query: 361 FSLISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIAVEGPYGPASMD 420
           FSLISSS+V+D+TMSIVVKCDKSWTRSLYD+IS+EREGES+QLKCISIAVEGPYGP+SMD
Sbjct: 379 FSLISSSNVDDETMSIVVKCDKSWTRSLYDVISSEREGESDQLKCISIAVEGPYGPSSMD 438

Query: 421 FLRYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKKSHDVSLLTSIL 480
           FLRYDHLLLIAGGIG+TP LSILQEI SMQ SS FKCPARIQLIHVMK S D+SLL SIL
Sbjct: 439 FLRYDHLLLIAGGIGITPFLSILQEINSMQNSSRFKCPARIQLIHVMKNSQDISLLNSIL 498

Query: 481 PLLLNRSDAKMYLQLKVFVTQEEESSTTVSELLNKFSQVQTIQSSTKCSSHAAQGLESLG 540
           PLLLN S+ KM LQLKVFVTQE +S  TV ELLNKFSQ+QTIQSSTKCSS+AAQGLES  
Sbjct: 499 PLLLNHSNKKMNLQLKVFVTQEVQSGATVVELLNKFSQMQTIQSSTKCSSYAAQGLESFN 558

Query: 541 GMATITGLTSIMFFVALVFFNHIVIPTEKASKKTKDRTPSWISDLLLMSSFALAILCGAV 600
            MA ITGLTSI+F + L+ FNHIVIPT+ ASKKTKDRTPSWISDLLLMSSF LAIL GAV
Sbjct: 559 WMAAITGLTSIVFLITLIIFNHIVIPTKTASKKTKDRTPSWISDLLLMSSFTLAILSGAV 618

Query: 601 VGIILRWRKLKKETVAVIQSQ--NQNAVPSSEARVSIQEEHEIQFGGRPNFKDILSDIQN 660
           VGI+LRWR+LKKE +A+IQ++   QNA+PSS  R S QEEHEI +GGRPNFK+I S+I++
Sbjct: 619 VGIVLRWRRLKKENLALIQTKTGTQNAIPSSVVRGSRQEEHEIYYGGRPNFKEIFSNIKH 678

Query: 661 DAGGSEIGVISCGPETMNMSVASACKEMCECLRRGSKKKKQKFNFHSLNFTL 709
           +  G+EIGVISCGPE MN+SVAS CK++ + LR+ SKKKK +F FHSLNFTL
Sbjct: 679 EDEGTEIGVISCGPEAMNVSVASTCKQISQGLRKDSKKKKPQFIFHSLNFTL 718

BLAST of MS001888 vs. ExPASy TrEMBL
Match: A0A6J1HMN3 (ferric reduction oxidase 8, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111464979 PE=4 SV=1)

HSP 1 Score: 1138.6 bits (2944), Expect = 0.0e+00
Identity = 587/712 (82.44%), Postives = 644/712 (90.45%), Query Frame = 0

Query: 1   MILLCAAWVSLWLLKPTDLWTRKWRLAENSARATVFGYYGLNFVVYTFPVIALVIIGLVY 60
           MILLCA WVSLWLLKPTDLWTRKWR+AEN+ARA+VFGYYGLNFVVYTFPV+ALVIIGLVY
Sbjct: 43  MILLCAGWVSLWLLKPTDLWTRKWRVAENTARASVFGYYGLNFVVYTFPVVALVIIGLVY 102

Query: 61  LNFKPSKPRTFRQ--FSTFSNALSNPLVVNSYLGVLSGTEILGVAFFLLFLVWTFYARIS 120
           LN K SKP+T RQ  FSTFSN LSNPLVVNSY+G+LSG E+LGVA FLLFL WTFYARIS
Sbjct: 103 LNIKHSKPQTCRQTKFSTFSNTLSNPLVVNSYVGILSGIELLGVALFLLFLAWTFYARIS 162

Query: 121 KDFRKLMPAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAPFRLIGIQFEAS 180
           KDFRKLMP ESLNL+IWELKYLRVATRFGLLAEACLGLLLLP+LRGLAPFRLIGIQFEAS
Sbjct: 163 KDFRKLMPEESLNLKIWELKYLRVATRFGLLAEACLGLLLLPVLRGLAPFRLIGIQFEAS 222

Query: 181 VKYHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQVTVLLCLSVANYMWKWQNTGRIYIA 240
           VKYH+WLGTAMVLF TFHGASTLFIWG+SNQIQDQV            WKWQNTGRIYIA
Sbjct: 223 VKYHVWLGTAMVLFGTFHGASTLFIWGISNQIQDQV------------WKWQNTGRIYIA 282

Query: 241 GEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF 300
           GEITLITGLVIWITSLPQIRR KFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF
Sbjct: 283 GEITLITGLVIWITSLPQIRRIKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF 342

Query: 301 GIDKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVKIPRISRFQWHA 360
            IDK+LRIIQSRTETCVVSA++LPCKAVELTLPKD RLKYKPTSVVYVKIPRISRF+WHA
Sbjct: 343 AIDKILRIIQSRTETCVVSARVLPCKAVELTLPKDHRLKYKPTSVVYVKIPRISRFEWHA 402

Query: 361 FSLISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIAVEGPYGPASMD 420
           FSLISSSSV+D+TMSIVVKC+KSWT+SLY++I+ EREGES+QLKCIS+AVEGPYGPAS+D
Sbjct: 403 FSLISSSSVDDETMSIVVKCNKSWTKSLYEIINTEREGESDQLKCISVAVEGPYGPASLD 462

Query: 421 FLRYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKKSHDVSLLTSIL 480
           FLRYDHL+LIAGGIGVTPLLSILQEITSM+ ++ F CPARIQLIHVMKKSH+++LL+SIL
Sbjct: 463 FLRYDHLILIAGGIGVTPLLSILQEITSMKNNTKFICPARIQLIHVMKKSHEIALLSSIL 522

Query: 481 PLLLNRSDAKMYLQLKVFVTQEEESSTTVSELLNKFSQVQTIQSSTKCSSHAAQGLESLG 540
           PLLL R D KM+LQLKVFVTQE +SS+T++ELLNKFSQ+QTIQSS  CSS+AA GLESLG
Sbjct: 523 PLLLKRLDGKMHLQLKVFVTQELQSSSTLTELLNKFSQMQTIQSSVNCSSYAAHGLESLG 582

Query: 541 GMATITGLTSIMFFVALVFFNHIVIPTEKASKKTKDRTPSWISDLLLMSSFALAILCGAV 600
            MA ITGL SI+FFVAL+ FNHIV+PT+KASKKTKDRTPSWISDLLLMSSFALAIL GAV
Sbjct: 583 WMAVITGLISIVFFVALIVFNHIVVPTKKASKKTKDRTPSWISDLLLMSSFALAILSGAV 642

Query: 601 VGIILRWRKLKKETVAVIQSQNQ--NAVPSSEARVSIQEEHEIQFGGRPNFKDILSDIQN 660
           VGI+LR R+LKK  +A+IQSQNQ  N  PSS AR S QEEHEI FG RPNF +I S+I N
Sbjct: 643 VGIVLRRRRLKKGNLALIQSQNQTKNTTPSSVARGSRQEEHEIYFGERPNFIEIFSNINN 702

Query: 661 DAGGSEIGVISCGPETMNMSVASACKEMCECLRRGSKKKKQKFNFHSLNFTL 709
            AG S+IGVIS GPE MN+SVA ACK+M    R+GSKK KQ+F+FHSLNFTL
Sbjct: 703 PAGESDIGVISSGPEAMNLSVAKACKQMSGGSRKGSKKNKQQFSFHSLNFTL 742

BLAST of MS001888 vs. ExPASy TrEMBL
Match: A0A6J1JIU9 (ferric reduction oxidase 8, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111484843 PE=4 SV=1)

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 585/712 (82.16%), Postives = 643/712 (90.31%), Query Frame = 0

Query: 1   MILLCAAWVSLWLLKPTDLWTRKWRLAENSARATVFGYYGLNFVVYTFPVIALVIIGLVY 60
           MILLCA WVSLWLLKPTDLWTRKWR+AEN+ARA+VFGYYGLNFVVYTFPVIALVIIGLVY
Sbjct: 19  MILLCAGWVSLWLLKPTDLWTRKWRVAENTARASVFGYYGLNFVVYTFPVIALVIIGLVY 78

Query: 61  LNFKPSKPRTFRQ--FSTFSNALSNPLVVNSYLGVLSGTEILGVAFFLLFLVWTFYARIS 120
           LN KPSKP+T RQ  FSTFSN LSNPLVVNSY+G+LSG E+LGVA FLLFL WTFYARIS
Sbjct: 79  LNIKPSKPQTCRQTKFSTFSNTLSNPLVVNSYVGILSGIELLGVALFLLFLAWTFYARIS 138

Query: 121 KDFRKLMPAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAPFRLIGIQFEAS 180
           KDFRKLMP ESLNL+IWELKYLRVATRFGLLAEACLGLLLLP+LRGLAPFRLIGIQFEAS
Sbjct: 139 KDFRKLMPEESLNLKIWELKYLRVATRFGLLAEACLGLLLLPVLRGLAPFRLIGIQFEAS 198

Query: 181 VKYHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQVTVLLCLSVANYMWKWQNTGRIYIA 240
           VKYH+WLGTAMVLF T HGASTLFIWG+SNQIQDQV            WKWQNTGRIYIA
Sbjct: 199 VKYHVWLGTAMVLFGTIHGASTLFIWGISNQIQDQV------------WKWQNTGRIYIA 258

Query: 241 GEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLF 300
           GEITLITGLVIWITSLPQIRR KFEIFYYTHHLYIVFFVFFLFHAGDRHFY++FPGLFLF
Sbjct: 259 GEITLITGLVIWITSLPQIRRIKFEIFYYTHHLYIVFFVFFLFHAGDRHFYIIFPGLFLF 318

Query: 301 GIDKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVKIPRISRFQWHA 360
            IDK+LRIIQSRTETCVVSA++LPCKAVELTLPKDQRLKYKPTSVVYVKIPRISRF+WHA
Sbjct: 319 AIDKILRIIQSRTETCVVSARVLPCKAVELTLPKDQRLKYKPTSVVYVKIPRISRFEWHA 378

Query: 361 FSLISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIAVEGPYGPASMD 420
           FSLISSSSV+D+T+SIVVKC+KSWT+SLY++I+ EREGES+QLKCIS+AVEGPYGPAS+D
Sbjct: 379 FSLISSSSVDDETISIVVKCNKSWTKSLYEIINTEREGESDQLKCISVAVEGPYGPASLD 438

Query: 421 FLRYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKKSHDVSLLTSIL 480
           FLRYDHL+LIAGGIGVTPLLSILQEITSM+ ++ F CPARIQLIHVMKKSH+V+LL+SIL
Sbjct: 439 FLRYDHLILIAGGIGVTPLLSILQEITSMKNNTKFICPARIQLIHVMKKSHEVALLSSIL 498

Query: 481 PLLLNRSDAKMYLQLKVFVTQEEESSTTVSELLNKFSQVQTIQSSTKCSSHAAQGLESLG 540
           PLLL R D KM+LQLKVFVTQE +S +T++ELLNKFSQ+QTI SS   SS+AA GLESLG
Sbjct: 499 PLLLKRLDGKMHLQLKVFVTQELQSGSTLTELLNKFSQMQTIHSSVNRSSYAAHGLESLG 558

Query: 541 GMATITGLTSIMFFVALVFFNHIVIPTEKASKKTKDRTPSWISDLLLMSSFALAILCGAV 600
            MA ITGL SI+FFVAL+ FNHIV+PTEKASKKTKDRTPSWISDLLLMSSFALAIL GAV
Sbjct: 559 WMAVITGLISIVFFVALIVFNHIVVPTEKASKKTKDRTPSWISDLLLMSSFALAILSGAV 618

Query: 601 VGIILRWRKLKKETVAVIQSQNQ--NAVPSSEARVSIQEEHEIQFGGRPNFKDILSDIQN 660
           VGI+LRWR+LKK  +A+IQSQNQ  N  PSS AR S QEEHEI FG RPNF +I S+I N
Sbjct: 619 VGIVLRWRRLKKCNLALIQSQNQTKNTTPSSVARGSRQEEHEIHFGERPNFIEIFSNINN 678

Query: 661 DAGGSEIGVISCGPETMNMSVASACKEMCECLRRGSKKKKQKFNFHSLNFTL 709
            AG S+IGVIS GPE MN+SVA ACK+M    R+GS+K KQ+F+FHSLNFTL
Sbjct: 679 PAGESDIGVISSGPEAMNLSVAKACKQMSGGSRKGSEKNKQQFSFHSLNFTL 718

BLAST of MS001888 vs. ExPASy TrEMBL
Match: A0A0A0KDJ2 (FAD-binding FR-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G093620 PE=4 SV=1)

HSP 1 Score: 1082.8 bits (2799), Expect = 0.0e+00
Identity = 561/710 (79.01%), Postives = 623/710 (87.75%), Query Frame = 0

Query: 1   MILLCAAWVSLWLLKPTDLWTRKWRLAENSARATVFGYYGLNFVVYTFPVIALVIIGLVY 60
           MILLCA W+SLWLLKPTDLWT+KW LAENSARA++FGYY     ++        II + +
Sbjct: 19  MILLCAGWISLWLLKPTDLWTKKWHLAENSARASLFGYYDPTPAIH-------YIIDMNF 78

Query: 61  LNFKPSKPRTFRQFSTFSNALSNPLVVNSYLGVLSGTEILGVAFFLLFLVWTFYARISKD 120
           + F+ +K      FSTFSNALSNPLVVN+YLG+LSG E+LGVA FLLFL WTFYARISKD
Sbjct: 79  MFFRQTK------FSTFSNALSNPLVVNNYLGILSGIELLGVALFLLFLAWTFYARISKD 138

Query: 121 FRKLMPAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAPFRLIGIQFEASVK 180
           FRKL+P ESLNL+IWELKYLR+ATRFGLLAEACL LLLLPILRGLAPF+LIGIQFEASVK
Sbjct: 139 FRKLLPVESLNLKIWELKYLRIATRFGLLAEACLSLLLLPILRGLAPFQLIGIQFEASVK 198

Query: 181 YHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQVTVLLCLSVANYMWKWQNTGRIYIAGE 240
           YH+WLGTAMVLF TFHG STLFIWG+SN+IQD+V            WKWQNTGRIYIAGE
Sbjct: 199 YHVWLGTAMVLFGTFHGISTLFIWGISNKIQDEV------------WKWQNTGRIYIAGE 258

Query: 241 ITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLFGI 300
           ITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLFGI
Sbjct: 259 ITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLFGI 318

Query: 301 DKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVKIPRISRFQWHAFS 360
           DK+LRIIQSR ETCVVSA+ILPCKAVELTLPKD+RLKYKPTSVVYVKIPRISRFQWHAFS
Sbjct: 319 DKILRIIQSRIETCVVSARILPCKAVELTLPKDRRLKYKPTSVVYVKIPRISRFQWHAFS 378

Query: 361 LISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIAVEGPYGPASMDFL 420
           LISSSSV+D+TMSIVVKCD+SWTRSLYD+IS EREGES+QLKCISIAVEGPYGP+SMDFL
Sbjct: 379 LISSSSVDDETMSIVVKCDESWTRSLYDVISTEREGESDQLKCISIAVEGPYGPSSMDFL 438

Query: 421 RYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKKSHDVSLLTSILPL 480
           RYDHLLLIAGGIG+TP LSILQEI SMQ SS FKCPARIQLIHVMK S D+SLL SILPL
Sbjct: 439 RYDHLLLIAGGIGITPFLSILQEINSMQNSSRFKCPARIQLIHVMKNSQDISLLNSILPL 498

Query: 481 LLNRSDAKMYLQLKVFVTQEEESSTTVSELLNKFSQVQTIQSSTKCSSHAAQGLESLGGM 540
           LLN S+ KM LQLKVFVTQE +S  TV ELLNKFSQ+QTIQSST+CSS+AAQGLESL  M
Sbjct: 499 LLNSSNKKMNLQLKVFVTQEVQSGATVVELLNKFSQMQTIQSSTRCSSYAAQGLESLNWM 558

Query: 541 ATITGLTSIMFFVALVFFNHIVIPTEKASKKTKDRTPSWISDLLLMSSFALAILCGAVVG 600
           A ITGLTSI+F VAL+ FNHIVIPT+KASKKTKDRTPSWISDLLLMSSF LAIL  AVVG
Sbjct: 559 AAITGLTSIVFLVALIIFNHIVIPTKKASKKTKDRTPSWISDLLLMSSFTLAILSSAVVG 618

Query: 601 IILRWRKLKKETVAVIQSQ--NQNAVPSSEARVSIQEEHEIQFGGRPNFKDILSDIQNDA 660
           I+LRWR+LKKE +A+IQ++   QNA+PSS  R S QEEHEI +GGRP FK+I S+I+++ 
Sbjct: 619 IVLRWRRLKKENLALIQTETGTQNAIPSSVVRGSRQEEHEIYYGGRPIFKEIFSNIKHED 678

Query: 661 GGSEIGVISCGPETMNMSVASACKEMCECLRRGSKKKKQKFNFHSLNFTL 709
            G+EIGVISCGP  MN+SVASACK++ + LR+ SKKK+Q+F FHSLNFTL
Sbjct: 679 EGTEIGVISCGPAAMNVSVASACKQISQGLRKDSKKKRQQFIFHSLNFTL 703

BLAST of MS001888 vs. TAIR 10
Match: AT5G50160.1 (ferric reduction oxidase 8 )

HSP 1 Score: 667.2 bits (1720), Expect = 1.5e-191
Identity = 356/737 (48.30%), Postives = 493/737 (66.89%), Query Frame = 0

Query: 1   MILLCAAWVSLWLLKPTDLWTRKWRLAENSARATVFGYYGLNFVVYTFPVIALVIIGLVY 60
           M LL   W+SLW++KPT +W + WR AE++AR T FGYYGLNF V++FP IAL I+GL+Y
Sbjct: 14  MNLLLIGWISLWIIKPTTIWIQSWRQAEDTARHTFFGYYGLNFAVFSFPPIALSIVGLIY 73

Query: 61  LNFKPSKPRTFRQFSTFSNALSNPLVVNSYLGVLSGTEILGVAFFLLFLVWTFYARISKD 120
           L+  P      R     +  +S P ++NS++G++S  EIL +  FLLFL W FYAR+S D
Sbjct: 74  LSLLPQHHHPTRGGRGAAITVSRPAIINSFIGIVSCFEILALLLFLLFLAWNFYARVSND 133

Query: 121 FRKLMPAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAPFRLIGIQFEASVK 180
           F+KLMP +++NL +W+LKY RVATRFGLLAEACL LLL P+LRGL+ FRL+ I+F ASVK
Sbjct: 134 FKKLMPVKTMNLNLWQLKYYRVATRFGLLAEACLSLLLFPVLRGLSMFRLLNIEFAASVK 193

Query: 181 YHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQVTVLLCLSVANYMWKWQNTGRIYIAGE 240
           YH+W GT ++ F+  HG STLFIW +++ I++++            WKWQ TGR+Y+AG 
Sbjct: 194 YHVWFGTGLIFFSLVHGGSTLFIWTITHHIEEEI------------WKWQRTGRVYVAGL 253

Query: 241 ITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFPGLFLFGI 300
           I+L+TGL++WITSLPQIRR+ FE+FYYTHHLYIVF V FLFHAGDRHFY V PG+FLFG+
Sbjct: 254 ISLVTGLLMWITSLPQIRRKNFEVFYYTHHLYIVFLVAFLFHAGDRHFYWVLPGMFLFGL 313

Query: 301 DKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVKIPRISRFQWHAFS 360
           DK+LRI+QSR+E+C++SA +  CKA+EL LPKD  L Y P+S +++ IP +SRFQWH FS
Sbjct: 314 DKILRIVQSRSESCILSANLFSCKAIELVLPKDPMLNYAPSSFIFLNIPLVSRFQWHPFS 373

Query: 361 LISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIAVEGPYGPASMDFL 420
           +ISSSSV+  ++SI++KC+  WT S+Y+ I  E     N++  I + VEGPYGPAS+DFL
Sbjct: 374 IISSSSVDKHSLSIMMKCEGDWTNSVYNKIE-EAANCENKINNIIVRVEGPYGPASVDFL 433

Query: 421 RYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKKSHDVSLLTSILPL 480
           RYD+L L+AGGIG+TP LSIL+E+ S    +  K P R+QL+  ++   D+++L  I  +
Sbjct: 434 RYDNLFLVAGGIGITPFLSILKELAS---KNRLKSPKRVQLVFAVRTFQDLNMLLPIASI 493

Query: 481 LLNRSDAKMYLQLKVFVTQEEESS---TTVSELLNKFSQVQTIQSST--KCSSHAAQGLE 540
           + N     + L+LKVFVTQE++ S   TT+ E L + SQVQ+I   T    S    +G E
Sbjct: 494 IFN-PIYNLNLKLKVFVTQEKKPSNGTTTLQEFLAQ-SQVQSIHLGTDEDYSRFPIRGPE 553

Query: 541 SLGGMATITGLTSIMFFVALVFFNHIVIPTEK-------------ASKKTKDRTPSWISD 600
           S   +AT+  +T + F   L+  +H  IP+E              A K  K++ PSW+ D
Sbjct: 554 SFRWLATLVLITVLTFLGFLIGLSHFFIPSEHKNHSGVMKLAASGAMKTAKEKVPSWVPD 613

Query: 601 LLLMSSFALAILCGAVVGIILRWRKLKKET-----VAVIQSQNQNAVPSSEARVSIQEEH 660
           L+++ S+ +AI  G     IL+ R+  KE        VI+ + +N        + I EEH
Sbjct: 614 LIIIVSYVIAISVGGFAATILQRRRKHKEAPRMSKEVVIKPEERNFTELKP--IPITEEH 673

Query: 661 EIQFGGRPNFKDILSDIQ-NDAGGSEIGVISCGPETMNMSVASACKEMCEC-----LRRG 709
           EI  G RP  ++I+S+ + N  G S +GV+ CGPE++  +VAS C++  +C     LRR 
Sbjct: 674 EIHIGERPKLEEIMSEFEKNLRGWSSVGVLVCGPESVKEAVASMCRQWPQCFGVEDLRR- 728

BLAST of MS001888 vs. TAIR 10
Match: AT1G01590.1 (ferric reduction oxidase 1 )

HSP 1 Score: 369.0 bits (946), Expect = 8.5e-102
Identity = 218/699 (31.19%), Postives = 381/699 (54.51%), Query Frame = 0

Query: 1   MILLCAAWVSLWLLKPTDLWTRKW--RLAENSARATVFGYYGLNFVVYTFPVIALVIIGL 60
           M+++    + +W++ PT  +   W   +     ++  +G  G+N +VY FP+I L  +G 
Sbjct: 19  MMVILMGTIVIWIMMPTSTYKEIWLTSMRAKLGKSIYYGRPGVNLLVYMFPMILLAFLGC 78

Query: 61  VYLNFKPSKPRTFRQFST-------FSNALSNPLVVNSYLGVLSGTEILGVAFFLLFLVW 120
           +YL+ K  K  T  QF++          AL  P++VN  LG+++ TE++ +  F+  L+W
Sbjct: 79  IYLHLK--KSTTVNQFNSGVEKKRAKFGALRRPMLVNGPLGIVTVTEVMFLTMFMALLLW 138

Query: 121 TFYARISKDFRKLM--PAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAPFR 180
           +    + + F  +    A +    +W+ +   +A R G++   CL  L  P+ RG +   
Sbjct: 139 SLANYMYRTFVNVTSESAATDGNNLWQARLDLIAVRIGIVGNICLAFLFYPVARGSSLLA 198

Query: 181 LIGIQFEASVKYHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQVTVLLCLSVANYMWKW 240
            +G+  E+S+KYHIWLG  +++  T HG      W   NQ+             + M +W
Sbjct: 199 AVGLTSESSIKYHIWLGHLVMIIFTSHGLCYFIYWISKNQL------------VSKMLEW 258

Query: 241 QNTGRIYIAGEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFY 300
             T    +AGEI L+ GL++W+T+ P+IRRR FE+F+Y+H+LYIVF +FF+FH G  H  
Sbjct: 259 DRTAVSNLAGEIALVAGLMMWVTTYPKIRRRLFEVFFYSHYLYIVFMLFFVFHVGISHAL 318

Query: 301 MVFPGLFLFGIDKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVKIP 360
           +  PG ++F +D+ LR +QSR    +VSA++LPC  VEL   K+  L Y PTS ++V IP
Sbjct: 319 IPLPGFYIFLVDRFLRFLQSRNNVKLVSARVLPCDTVELNFSKNPMLMYSPTSTMFVNIP 378

Query: 361 RISRFQWHAFSLISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIAVE 420
            IS+ QWH F++ISSS +  +T+S+++K    W+  LYDM+S+     S+Q+  ++++VE
Sbjct: 379 SISKLQWHPFTIISSSKLEPETLSVMIKSQGKWSTKLYDMLSS---SSSDQINRLAVSVE 438

Query: 421 GPYGPASMDFLRYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKKSH 480
           GPYGP+S DFLR++ L++++GG G+TP +SI++++  M  +   K P ++ LI   K S 
Sbjct: 439 GPYGPSSTDFLRHESLVMVSGGSGITPFISIVRDLFYMSSTHKCKIP-KMTLICAFKNSS 498

Query: 481 DVSLLTSILPLLLNRSDAKMY--LQLKVFVTQEEESSTTVSELLNKFSQVQTIQSSTKCS 540
           D+S+L  ILP     +D   +  +Q+K FVT+EE+  T+V E  +  + ++T       S
Sbjct: 499 DLSMLDLILPTSGLTTDMASFVDIQIKAFVTREEK--TSVKESTHNRNIIKTRHFKPNVS 558

Query: 541 SHAAQGLESLGGMATITGLTSIMFFVALVFF----NHIVIPTEKASKKTKDRTPSW-ISD 600
                 +        +  + S  F + +V       + + P ++ S+K      +W    
Sbjct: 559 DQPISPILGPNSWLCLAAILSSSFMIFIVIIAIITRYHIHPIDQNSEKY-----TWAYKS 618

Query: 601 LLLMSSFALAILCGAVVGIILRWRKLKKETVAVIQSQNQNAVPSSEARVSIQEEHEIQFG 660
           L+ + S ++ ++  +   ++   +K   +    + + +   + SS  ++ I +  +I +G
Sbjct: 619 LIYLVSISITVVTTSTAAMLWNKKKYYAKNDQYVDNLSPVIIESSPQQL-ISQSTDIHYG 678

Query: 661 GRPNFKDILSDIQNDAGGSEIGVISCGPETMNMSVASAC 682
            RPN   +L  ++    GS +G++ CGP+ M   VA  C
Sbjct: 679 ERPNLNKLLVGLK----GSSVGILVCGPKKMRQKVAKIC 687

BLAST of MS001888 vs. TAIR 10
Match: AT1G23020.1 (ferric reduction oxidase 3 )

HSP 1 Score: 364.4 bits (934), Expect = 2.1e-100
Identity = 223/700 (31.86%), Postives = 385/700 (55.00%), Query Frame = 0

Query: 1   MILLCAAWVSLWLLKPTDLWTRKW--RLAENSARATVFGYYGLNFVVYTFPVIALVIIGL 60
           M++L    V +W++ PT  + + W   +     ++  FG  G+N +VY FP+I L  +G 
Sbjct: 43  MVILMGT-VVIWIMMPTSTYKKIWLKSMRAKLGKSIYFGKPGVNLLVYMFPMILLASLGS 102

Query: 61  VYLNFKPSKPRTFRQFSTFS-------NALSNPLVVNSYLGVLSGTEILGVAFFLLFLVW 120
           +YL+ K  K     QF+           AL  P++V + LG+++ TE++ +  F+  L+W
Sbjct: 103 IYLHLK--KQTRVNQFNRMDRKKIDKFGALKRPMLVKAGLGIVTVTEVMFLMMFMALLLW 162

Query: 121 T----FYARISKDFRKLMPAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAP 180
           +    FY        + +P +  NL  W+ +   +A R GL    CLG L  P+ RG + 
Sbjct: 163 SLANYFYHTFVTITPQSLPTDGDNL--WQARLDSIAVRLGLTGNICLGFLFYPVARGSSL 222

Query: 181 FRLIGIQFEASVKYHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQVTVLLCLSVANYMW 240
              +G+  E+S KYHIWLG  ++   T HG      W  +NQ+             + M 
Sbjct: 223 LAAVGLTSESSTKYHIWLGNLVMTLFTSHGLCYCIYWISTNQV-------------SQML 282

Query: 241 KWQNTGRIYIAGEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRH 300
           +W  TG  ++AGEI L+ GL++W T+ P IRRR FE+F+YTH+LY+VF +FF+FH G  +
Sbjct: 283 EWDRTGISHLAGEIALVAGLLMWATTFPAIRRRFFEVFFYTHYLYMVFMLFFVFHVGISY 342

Query: 301 FYMVFPGLFLFGIDKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVK 360
             + FPG ++F +D+ LR +QSR    +VSA++LPC+ VEL   K+  L Y PTS+++V 
Sbjct: 343 ALISFPGFYIFMVDRFLRFLQSRNNVKLVSARVLPCETVELNFSKNPMLMYSPTSILFVN 402

Query: 361 IPRISRFQWHAFSLISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIA 420
           IP IS+ QWH F++ SSS +    +S+++K    W+  L+ M+++     SNQ+  ++++
Sbjct: 403 IPSISKLQWHPFTITSSSKLEPKKLSVMIKSQGKWSSKLHHMLAS-----SNQIDHLAVS 462

Query: 421 VEGPYGPASMDFLRYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKK 480
           VEGPYGPAS D+LR+D L++++GG G+TP +SI++++  +  ++ +K P +I LI   K 
Sbjct: 463 VEGPYGPASTDYLRHDSLVMVSGGSGITPFISIIRDLLYVSSTNAYKTP-KITLICAFKN 522

Query: 481 SHDVSLLTSILPLLLNRSDAKMY--LQLKVFVTQEEESSTTVSELLNKFSQVQTIQSSTK 540
           S D+S+L  ILP   N ++   +  +Q+K FVT+E+ S+  +       + ++T+     
Sbjct: 523 SSDLSMLNLILP---NSTEISSFIDIQIKAFVTREKVSTCNM-------NIIKTLSFKPY 582

Query: 541 CSSHAAQ---GLESLGGMATITGLTSIMFFVAL-VFFNHIVIPTEKASKKTKDRTPSWIS 600
            S        G  S   +ATI   + ++F + + +   + + P +++SK+      S  +
Sbjct: 583 VSDQPISPILGPNSWLWLATILSSSFMIFIIIIAIISRYHIYPIDQSSKE----YTSAYT 642

Query: 601 DLLLMSSFALAILCGAVVGIILRWRKLKKETVAVIQSQNQNAVPSSEARVSIQEEHEIQF 660
            L+ + + +++++  + V ++   +   K     + + +   + SS  ++ + E   I +
Sbjct: 643 SLIYLLAISISVVATSTVAMLCNKKSYFKGLYQNVDALSPLMIESSPDQL-LPEFTNIHY 699

Query: 661 GGRPNFKDILSDIQNDAGGSEIGVISCGPETMNMSVASAC 682
           G RPN   +L  ++    GS +GV+ CGP  M   VA  C
Sbjct: 703 GERPNLNKLLVGLK----GSSVGVLVCGPRKMREEVAKIC 699

BLAST of MS001888 vs. TAIR 10
Match: AT1G23020.2 (ferric reduction oxidase 3 )

HSP 1 Score: 363.6 bits (932), Expect = 3.6e-100
Identity = 223/701 (31.81%), Postives = 387/701 (55.21%), Query Frame = 0

Query: 1   MILLCAAWVSLWLLKPTDLWTRKW--RLAENSARATVFGYYGLNFVVYTFPVIALVIIGL 60
           M++L    V +W++ PT  + + W   +     ++  FG  G+N +VY FP+I L  +G 
Sbjct: 43  MVILMGT-VVIWIMMPTSTYKKIWLKSMRAKLGKSIYFGKPGVNLLVYMFPMILLASLGS 102

Query: 61  VYLNFKPSKPRTFRQFSTFSN--------ALSNPLVVNSYLGVLSGTEILGVAFFLLFLV 120
           +YL+ K  K     QF++  +        AL  P++V + LG+++ TE++ +  F+  L+
Sbjct: 103 IYLHLK--KQTRVNQFNSRMDRKKIDKFGALKRPMLVKAGLGIVTVTEVMFLMMFMALLL 162

Query: 121 WT----FYARISKDFRKLMPAESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLA 180
           W+    FY        + +P +  NL  W+ +   +A R GL    CLG L  P+ RG +
Sbjct: 163 WSLANYFYHTFVTITPQSLPTDGDNL--WQARLDSIAVRLGLTGNICLGFLFYPVARGSS 222

Query: 181 PFRLIGIQFEASVKYHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQVTVLLCLSVANYM 240
               +G+  E+S KYHIWLG  ++   T HG      W  +NQ+             + M
Sbjct: 223 LLAAVGLTSESSTKYHIWLGNLVMTLFTSHGLCYCIYWISTNQV-------------SQM 282

Query: 241 WKWQNTGRIYIAGEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDR 300
            +W  TG  ++AGEI L+ GL++W T+ P IRRR FE+F+YTH+LY+VF +FF+FH G  
Sbjct: 283 LEWDRTGISHLAGEIALVAGLLMWATTFPAIRRRFFEVFFYTHYLYMVFMLFFVFHVGIS 342

Query: 301 HFYMVFPGLFLFGIDKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYV 360
           +  + FPG ++F +D+ LR +QSR    +VSA++LPC+ VEL   K+  L Y PTS+++V
Sbjct: 343 YALISFPGFYIFMVDRFLRFLQSRNNVKLVSARVLPCETVELNFSKNPMLMYSPTSILFV 402

Query: 361 KIPRISRFQWHAFSLISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISI 420
            IP IS+ QWH F++ SSS +    +S+++K    W+  L+ M+++     SNQ+  +++
Sbjct: 403 NIPSISKLQWHPFTITSSSKLEPKKLSVMIKSQGKWSSKLHHMLAS-----SNQIDHLAV 462

Query: 421 AVEGPYGPASMDFLRYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMK 480
           +VEGPYGPAS D+LR+D L++++GG G+TP +SI++++  +  ++ +K P +I LI   K
Sbjct: 463 SVEGPYGPASTDYLRHDSLVMVSGGSGITPFISIIRDLLYVSSTNAYKTP-KITLICAFK 522

Query: 481 KSHDVSLLTSILPLLLNRSDAKMY--LQLKVFVTQEEESSTTVSELLNKFSQVQTIQSST 540
            S D+S+L  ILP   N ++   +  +Q+K FVT+E+ S+  +       + ++T+    
Sbjct: 523 NSSDLSMLNLILP---NSTEISSFIDIQIKAFVTREKVSTCNM-------NIIKTLSFKP 582

Query: 541 KCSSHAAQ---GLESLGGMATITGLTSIMFFVAL-VFFNHIVIPTEKASKKTKDRTPSWI 600
             S        G  S   +ATI   + ++F + + +   + + P +++SK+      S  
Sbjct: 583 YVSDQPISPILGPNSWLWLATILSSSFMIFIIIIAIISRYHIYPIDQSSKE----YTSAY 642

Query: 601 SDLLLMSSFALAILCGAVVGIILRWRKLKKETVAVIQSQNQNAVPSSEARVSIQEEHEIQ 660
           + L+ + + +++++  + V ++   +   K     + + +   + SS  ++ + E   I 
Sbjct: 643 TSLIYLLAISISVVATSTVAMLCNKKSYFKGLYQNVDALSPLMIESSPDQL-LPEFTNIH 700

Query: 661 FGGRPNFKDILSDIQNDAGGSEIGVISCGPETMNMSVASAC 682
           +G RPN   +L  ++    GS +GV+ CGP  M   VA  C
Sbjct: 703 YGERPNLNKLLVGLK----GSSVGVLVCGPRKMREEVAKIC 700

BLAST of MS001888 vs. TAIR 10
Match: AT1G01580.1 (ferric reduction oxidase 2 )

HSP 1 Score: 359.4 bits (921), Expect = 6.7e-99
Identity = 222/732 (30.33%), Postives = 383/732 (52.32%), Query Frame = 0

Query: 1   MILLCAAWVSLWLLKPTDLWTRKW--RLAENSARATVFGYYGLNFVVYTFPVIALVIIGL 60
           M+++    + LW++ PT  +  KW   L      +T FG  G    +Y FP++ +  +G 
Sbjct: 31  MMVIFLGTIMLWIMMPTLTYRTKWLPHLRIKFGTSTYFGATGTTLFMYMFPMMVVACLGC 90

Query: 61  VYLNFKPSKPRTFRQFSTFS---NALSNPLVVNSYLGVLSGTEILGVAFFLLFLVWTFYA 120
           VYL+FK  K        T     + L  P++V   LG++S TEI  +A F+  L+W F  
Sbjct: 91  VYLHFKNRKSPHHIDRETKGGVWSKLRKPMLVKGPLGIVSVTEITFLAMFVALLLWCFIT 150

Query: 121 RISKDFRKLMP--AESLNLQIWELKYLRVATRFGLLAEACLGLLLLPILRGLAPFRLIGI 180
            +   F  + P  A + +  +W+ K    A R GL+   CL  L LP+ RG +    +G+
Sbjct: 151 YLRNSFATITPKSAAAHDESLWQAKLESAALRLGLIGNICLAFLFLPVARGSSLLPAMGL 210

Query: 181 QFEASVKYHIWLGTAMVLFATFHGASTLFIWGLSNQIQDQVTVLLCLSVANYMWKWQNTG 240
             E+S+KYHIWLG  ++   T HG   +  W   ++I   +              W   G
Sbjct: 211 TSESSIKYHIWLGHMVMALFTVHGLCYIIYWASMHEISQMI-------------MWDTKG 270

Query: 241 RIYIAGEITLITGLVIWITSLPQIRRRKFEIFYYTHHLYIVFFVFFLFHAGDRHFYMVFP 300
              +AGEI L  GLV+W T+ P+IRRR FE+F+YTH+LYIVF +FF+ H G    ++  P
Sbjct: 271 VSNLAGEIALAAGLVMWATTYPKIRRRFFEVFFYTHYLYIVFMLFFVLHVGISFSFIALP 330

Query: 301 GLFLFGIDKVLRIIQSRTETCVVSAQILPCKAVELTLPKDQRLKYKPTSVVYVKIPRISR 360
           G ++F +D+ LR +QSR    +++A+ILP   +ELT  K+ +L Y PTS+++V IP IS+
Sbjct: 331 GFYIFLVDRFLRFLQSRENVRLLAARILPSDTMELTFSKNSKLVYSPTSIMFVNIPSISK 390

Query: 361 FQWHAFSLISSSSVNDDTMSIVVKCDKSWTRSLYDMISAEREGESNQLKCISIAVEGPYG 420
            QWH F++ SSS +  + +SIV+K +  W+  L+  +S+     S+Q+  ++++VEGPYG
Sbjct: 391 LQWHPFTITSSSKLEPEKLSIVIKKEGKWSTKLHQRLSS-----SDQIDRLAVSVEGPYG 450

Query: 421 PASMDFLRYDHLLLIAGGIGVTPLLSILQEITSMQKSSNFKCPARIQLIHVMKKSHDVSL 480
           PAS DFLR++ L+++ GG G+TP +S+++++ +  +    K P +I LI   KKS ++S+
Sbjct: 451 PASADFLRHEALVMVCGGSGITPFISVIRDLIATSQKETCKIP-KITLICAFKKSSEISM 510

Query: 481 LTSILPL--LLNRSDAKMYLQLKVFVTQEEESSTTVSELLNKFSQVQTIQSSTKCSSHAA 540
           L  +LPL  L     + + ++++ F+T++ ++         K  +++T+      S  + 
Sbjct: 511 LDLVLPLSGLETELSSDINIKIEAFITRDNDAGDEA-----KAGKIKTLWFKPSLSDQSI 570

Query: 541 Q---GLESLGGMATITGLTSIMFFVAL-VFFNHIVIPTEKASKKTKDRTPSWISDLLLMS 600
               G  S   +  I   + ++F + + +   + + P +  + K    T   I  +L++S
Sbjct: 571 SSILGPNSWLWLGAILASSFLIFMIIIGIITRYYIYPIDHNTNKIYSLTSKTIIYILVIS 630

Query: 601 SFALAILCGAVVGIILRWRKLKKETVAVIQSQNQNAVPSSE------------ARVSIQE 660
              +A    A++    ++ K++ + V  +   +  + P+S              + S+ +
Sbjct: 631 VSIMATCSAAMLWNKKKYGKVESKQVQNVDRPSPTSSPTSSWGYNSLREIESTPQESLVQ 690

Query: 661 EHEIQFGGRPNFKDILSDIQNDAGGSEIGVISCGPETMNMSVASACKEMCECLRRGSKKK 708
              + FG RPN K +L D++    GS +GV+ CGP+ M   VA  C          S   
Sbjct: 691 RTNLHFGERPNLKKLLLDVE----GSSVGVLVCGPKKMRQKVAEIC----------SSGL 724

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135163.10.0e+0097.18ferric reduction oxidase 8, mitochondrial [Momordica charantia][more]
XP_008459876.10.0e+0082.44PREDICTED: ferric reduction oxidase 8, mitochondrial [Cucumis melo] >XP_00845987... [more]
XP_011656798.10.0e+0082.16ferric reduction oxidase 8, mitochondrial [Cucumis sativus][more]
XP_022965033.10.0e+0082.44ferric reduction oxidase 8, mitochondrial [Cucurbita moschata][more]
XP_022987228.10.0e+0082.16ferric reduction oxidase 8, mitochondrial [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8VY132.1e-19048.30Ferric reduction oxidase 8, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FRO... [more]
Q9LMM21.2e-10031.19Probable ferric reduction oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=FRO1 PE=2... [more]
F4I4K75.0e-9931.81Ferric reduction oxidase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FRO... [more]
P929499.5e-9830.33Ferric reduction oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=FRO2 PE=1 SV=2[more]
Q8W1108.9e-8829.66Ferric reduction oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=FRO4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1C4160.0e+0097.18ferric reduction oxidase 8, mitochondrial OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A1S3CBN30.0e+0082.44ferric reduction oxidase 8, mitochondrial OS=Cucumis melo OX=3656 GN=LOC10349886... [more]
A0A6J1HMN30.0e+0082.44ferric reduction oxidase 8, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1JIU90.0e+0082.16ferric reduction oxidase 8, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A0A0KDJ20.0e+0079.01FAD-binding FR-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_... [more]
Match NameE-valueIdentityDescription
AT5G50160.11.5e-19148.30ferric reduction oxidase 8 [more]
AT1G01590.18.5e-10231.19ferric reduction oxidase 1 [more]
AT1G23020.12.1e-10031.86ferric reduction oxidase 3 [more]
AT1G23020.23.6e-10031.81ferric reduction oxidase 3 [more]
AT1G01580.16.7e-9930.33ferric reduction oxidase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000778Cytochrome b245, heavy chainPRINTSPR00466GP91PHOXcoord: 237..260
score: 29.17
coord: 262..282
score: 52.38
IPR013112FAD-binding 8PFAMPF08022FAD_binding_8coord: 316..416
e-value: 2.6E-21
score: 75.7
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainGENE3D3.40.50.80coord: 593..706
e-value: 3.7E-13
score: 51.8
coord: 406..542
e-value: 2.1E-20
score: 75.4
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainSUPERFAMILY52343Ferredoxin reductase-like, C-terminal NADP-linked domaincoord: 406..682
IPR013121Ferric reductase, NAD binding domainPFAMPF08030NAD_binding_6coord: 422..681
e-value: 8.1E-26
score: 91.0
IPR013130Ferric reductase transmembrane component-like domainPFAMPF01794Ferric_reductcoord: 147..279
e-value: 4.2E-17
score: 62.4
NoneNo IPR availableSFLDSFLDG01168Ferric_reductase_subgroup_(FRcoord: 44..708
e-value: 0.0
score: 407.3
NoneNo IPR availableSFLDSFLDS00052Ferric_Reductase_Domaincoord: 17..708
e-value: 0.0
score: 415.7
NoneNo IPR availablePANTHERPTHR11972:SF155FERRIC REDUCTION OXIDASE 8, MITOCHONDRIALcoord: 1..707
NoneNo IPR availablePANTHERPTHR11972NADPH OXIDASEcoord: 1..707
NoneNo IPR availableCDDcd06186NOX_Duox_like_FAD_NADPcoord: 315..470
e-value: 6.70805E-38
score: 138.206
IPR017927FAD-binding domain, ferredoxin reductase-typePROSITEPS51384FAD_FRcoord: 308..418
score: 11.681367

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS001888.1MS001888.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0016491 oxidoreductase activity