MS001836 (gene) Bitter gourd (TR) v1

BLAST of MS001836 vs. NCBI nr
Match: XP_022134926.1 (non-specific phospholipase C6 [Momordica charantia])

HSP 1 Score: 1068.9 bits (2763), Expect = 1.4e-308
Identity = 520/520 (100.00%), Postives = 520/520 (100.00%), Query Frame = 0

Query: 1   MRGRRNKPPFHLPFIFLFLFFLTLSWVPQRICQQPIKTVVVLVMENRSFDHMIGWMKKSI 60
           MRGRRNKPPFHLPFIFLFLFFLTLSWVPQRICQQPIKTVVVLVMENRSFDHMIGWMKKSI
Sbjct: 1   MRGRRNKPPFHLPFIFLFLFFLTLSWVPQRICQQPIKTVVVLVMENRSFDHMIGWMKKSI 60

Query: 61  NPQINGVTGDECNPVSTNNSNPGTICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMSG 120
           NPQINGVTGDECNPVSTNNSNPGTICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMSG
Sbjct: 61  NPQINGVTGDECNPVSTNNSNPGTICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMSG 120

Query: 121 FVEQALSMSPNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSAT 180
           FVEQALSMSPNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSAT
Sbjct: 121 FVEQALSMSPNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSAT 180

Query: 181 SHGSTSHVKKQLALGYPQKTIFDSLHENGHDFGIYFQNIPTTLFYRNLRKLKYIFKFHQY 240
           SHGSTSHVKKQLALGYPQKTIFDSLHENGHDFGIYFQNIPTTLFYRNLRKLKYIFKFHQY
Sbjct: 181 SHGSTSHVKKQLALGYPQKTIFDSLHENGHDFGIYFQNIPTTLFYRNLRKLKYIFKFHQY 240

Query: 241 DLKFKKDARNGKLPSLSVIEPRYFDLVGFPANDDHPSHDVANGQKLVKEVYETLRASPQW 300
           DLKFKKDARNGKLPSLSVIEPRYFDLVGFPANDDHPSHDVANGQKLVKEVYETLRASPQW
Sbjct: 241 DLKFKKDARNGKLPSLSVIEPRYFDLVGFPANDDHPSHDVANGQKLVKEVYETLRASPQW 300

Query: 301 NETLLIITYDEHGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIKK 360
           NETLLIITYDEHGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIKK
Sbjct: 301 NETLLIITYDEHGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIKK 360

Query: 361 GTVISSPNGPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEHIVGQLTSPRTDC 420
           GTVISSPNGPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEHIVGQLTSPRTDC
Sbjct: 361 GTVISSPNGPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEHIVGQLTSPRTDC 420

Query: 421 PVTLPDVTPLRKTEANENSGLSEFQSEVVQLAAVLNGDHFLSTFPNEISKKMSVKEAHDY 480
           PVTLPDVTPLRKTEANENSGLSEFQSEVVQLAAVLNGDHFLSTFPNEISKKMSVKEAHDY
Sbjct: 421 PVTLPDVTPLRKTEANENSGLSEFQSEVVQLAAVLNGDHFLSTFPNEISKKMSVKEAHDY 480

Query: 481 TRGAVARFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN 521
           TRGAVARFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN
Sbjct: 481 TRGAVARFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN 520

BLAST of MS001836 vs. NCBI nr
Match: XP_023515458.1 (non-specific phospholipase C6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 988.8 bits (2555), Expect = 1.8e-284
Identity = 487/521 (93.47%), Postives = 500/521 (95.97%), Query Frame = 0

Query: 1   MRGRRNKPPFHLPFIFLFLFFLTLSWVPQR-ICQQPIKTVVVLVMENRSFDHMIGWMKKS 60
           MRGRR+K   HLPFIF  L  LTLSW+PQR   QQPIKTVVVLVMENRSFDHMIGWMKK 
Sbjct: 1   MRGRRSKVTLHLPFIFFLL--LTLSWIPQRTFQQQPIKTVVVLVMENRSFDHMIGWMKKY 60

Query: 61  INPQINGVTGDECNPVSTNNSNPGTICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS 120
           I+P+INGVTGDECNPVST N NP TICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS
Sbjct: 61  IDPRINGVTGDECNPVSTKNPNPETICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS 120

Query: 121 GFVEQALSMSPNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA 180
           GFVEQALSMS NLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA
Sbjct: 121 GFVEQALSMSSNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA 180

Query: 181 TSHGSTSHVKKQLALGYPQKTIFDSLHENGHDFGIYFQNIPTTLFYRNLRKLKYIFKFHQ 240
           TSHGSTSHVKKQLALGYPQKTIFDSLHENG DFG+YFQNIPTTLFYRNLRKLKYIFKFH 
Sbjct: 181 TSHGSTSHVKKQLALGYPQKTIFDSLHENGLDFGVYFQNIPTTLFYRNLRKLKYIFKFHP 240

Query: 241 YDLKFKKDARNGKLPSLSVIEPRYFDLVGFPANDDHPSHDVANGQKLVKEVYETLRASPQ 300
           YDL+FKKDARNGKLPSL+VIEPRYFDLVG PANDDHPSHDVANGQKLVKEVYETLRASPQ
Sbjct: 241 YDLQFKKDARNGKLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQ 300

Query: 301 WNETLLIITYDEHGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK 360
           WN+TLLIITYDEHGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK
Sbjct: 301 WNQTLLIITYDEHGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK 360

Query: 361 KGTVISSPNGPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEHIVGQLTSPRTD 420
           KGTVISSP GPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFE IVGQLTSPRTD
Sbjct: 361 KGTVISSPKGPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTD 420

Query: 421 CPVTLPDVTPLRKTEANENSGLSEFQSEVVQLAAVLNGDHFLSTFPNEISKKMSVKEAHD 480
           CPVTLP+VTPLRKTEANENS LSEFQSEVVQLAAVLNGDHFLS+FPNEIS+KM+VKEAHD
Sbjct: 421 CPVTLPEVTPLRKTEANENSRLSEFQSEVVQLAAVLNGDHFLSSFPNEISEKMTVKEAHD 480

Query: 481 YTRGAVARFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN 521
           YTRGAV+RFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN
Sbjct: 481 YTRGAVSRFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN 519

BLAST of MS001836 vs. NCBI nr
Match: XP_004138043.1 (non-specific phospholipase C6 [Cucumis sativus] >KGN63477.1 hypothetical protein Csa_014262 [Cucumis sativus])

HSP 1 Score: 987.3 bits (2551), Expect = 5.2e-284
Identity = 485/521 (93.09%), Postives = 501/521 (96.16%), Query Frame = 0

Query: 1   MRGRRNKPPFHLPFIFLFLFFLTLSWVPQR-ICQQPIKTVVVLVMENRSFDHMIGWMKKS 60
           MRGR++K  FHLP IF  L  LTLSW+PQR   QQPIKTVVVLVMENRSFDHMIGWMKK 
Sbjct: 1   MRGRQSKFHFHLPSIFFLL--LTLSWLPQRSFQQQPIKTVVVLVMENRSFDHMIGWMKKY 60

Query: 61  INPQINGVTGDECNPVSTNNSNPGTICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS 120
           INPQINGVTGDECNPVST N NP TICF+DDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS
Sbjct: 61  INPQINGVTGDECNPVSTKNPNPETICFTDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS 120

Query: 121 GFVEQALSMSPNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA 180
           GFVEQALSMSPNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA
Sbjct: 121 GFVEQALSMSPNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA 180

Query: 181 TSHGSTSHVKKQLALGYPQKTIFDSLHENGHDFGIYFQNIPTTLFYRNLRKLKYIFKFHQ 240
           TSHGSTSHVKKQLA+GYPQKTIFDSLHENG +FGIYFQNIPTTLFYRNLRKLKYIFKFHQ
Sbjct: 181 TSHGSTSHVKKQLAMGYPQKTIFDSLHENGINFGIYFQNIPTTLFYRNLRKLKYIFKFHQ 240

Query: 241 YDLKFKKDARNGKLPSLSVIEPRYFDLVGFPANDDHPSHDVANGQKLVKEVYETLRASPQ 300
           YDLKFKKDARNGKLPSL+VIEPRYFDLVG PANDDHPSHDVANGQKLVKEVYETLRASPQ
Sbjct: 241 YDLKFKKDARNGKLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQ 300

Query: 301 WNETLLIITYDEHGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK 360
           WNETLLIITYDEHGGF+DHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK
Sbjct: 301 WNETLLIITYDEHGGFYDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK 360

Query: 361 KGTVISSPNGPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEHIVGQLTSPRTD 420
           KGTVISSP GPTPNSEFEHSSIPATIKKIFN+ SNFLTHRDAWAGTFE IV QLTSPRTD
Sbjct: 361 KGTVISSPKGPTPNSEFEHSSIPATIKKIFNIPSNFLTHRDAWAGTFEDIVDQLTSPRTD 420

Query: 421 CPVTLPDVTPLRKTEANENSGLSEFQSEVVQLAAVLNGDHFLSTFPNEISKKMSVKEAHD 480
           CPVTLP+VTPLRKTEANENSGLSEFQSEVVQLAAVLNGDHFLS+FPNEIS+KM++KEAHD
Sbjct: 421 CPVTLPEVTPLRKTEANENSGLSEFQSEVVQLAAVLNGDHFLSSFPNEISEKMTIKEAHD 480

Query: 481 YTRGAVARFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN 521
           YTRGAV+RFIRASKEAIKLGADESAIVDMRSSLTTRSS+HN
Sbjct: 481 YTRGAVSRFIRASKEAIKLGADESAIVDMRSSLTTRSSIHN 519

BLAST of MS001836 vs. NCBI nr
Match: XP_022921419.1 (non-specific phospholipase C6-like [Cucurbita moschata])

HSP 1 Score: 986.5 bits (2549), Expect = 8.9e-284
Identity = 485/521 (93.09%), Postives = 500/521 (95.97%), Query Frame = 0

Query: 1   MRGRRNKPPFHLPFIFLFLFFLTLSWVPQR-ICQQPIKTVVVLVMENRSFDHMIGWMKKS 60
           MRG R+K   HLPFIF  L  LTLSW+PQR   QQPIKTVVVLVMENRSFDHMIGWMKK 
Sbjct: 1   MRGGRSKVTVHLPFIFFLL--LTLSWIPQRTFQQQPIKTVVVLVMENRSFDHMIGWMKKY 60

Query: 61  INPQINGVTGDECNPVSTNNSNPGTICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS 120
           I+P+INGVTGDECNPVST N NP TICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS
Sbjct: 61  IDPRINGVTGDECNPVSTKNPNPETICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS 120

Query: 121 GFVEQALSMSPNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA 180
           GFVEQALSMS NLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA
Sbjct: 121 GFVEQALSMSSNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA 180

Query: 181 TSHGSTSHVKKQLALGYPQKTIFDSLHENGHDFGIYFQNIPTTLFYRNLRKLKYIFKFHQ 240
           TSHGSTSHVKKQLALGYPQKTIFDSLHENG DFG+YFQNIPTTLFYRNLRKLKYIFKFH 
Sbjct: 181 TSHGSTSHVKKQLALGYPQKTIFDSLHENGLDFGVYFQNIPTTLFYRNLRKLKYIFKFHP 240

Query: 241 YDLKFKKDARNGKLPSLSVIEPRYFDLVGFPANDDHPSHDVANGQKLVKEVYETLRASPQ 300
           YDL+FKKDARNGKLPSL+VIEPRYFDLVG PANDDHPSHDVANGQKLVKEVYETLRASPQ
Sbjct: 241 YDLQFKKDARNGKLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQ 300

Query: 301 WNETLLIITYDEHGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK 360
           WN+TLLIITYDEHGGFFDHV+TPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK
Sbjct: 301 WNQTLLIITYDEHGGFFDHVRTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK 360

Query: 361 KGTVISSPNGPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEHIVGQLTSPRTD 420
           KGTVISSP GPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFE IVGQLTSPRTD
Sbjct: 361 KGTVISSPKGPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTD 420

Query: 421 CPVTLPDVTPLRKTEANENSGLSEFQSEVVQLAAVLNGDHFLSTFPNEISKKMSVKEAHD 480
           CPVTLP+VTPLRKTEANENSG+SEFQSEVVQLAAVLNGDHFLS+FPNEIS+KM+VKEAHD
Sbjct: 421 CPVTLPEVTPLRKTEANENSGVSEFQSEVVQLAAVLNGDHFLSSFPNEISEKMTVKEAHD 480

Query: 481 YTRGAVARFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN 521
           YTRGAV+RFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN
Sbjct: 481 YTRGAVSRFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN 519

BLAST of MS001836 vs. NCBI nr
Match: XP_022987132.1 (non-specific phospholipase C6-like [Cucurbita maxima])

HSP 1 Score: 986.1 bits (2548), Expect = 1.2e-283
Identity = 486/521 (93.28%), Postives = 499/521 (95.78%), Query Frame = 0

Query: 1   MRGRRNKPPFHLPFIFLFLFFLTLSWVPQR-ICQQPIKTVVVLVMENRSFDHMIGWMKKS 60
           MR RR+K   HLPFIF  L  LTLSW+PQR   QQPIKTVVVLVMENRSFDHMIGWMKK 
Sbjct: 1   MRRRRSKVTVHLPFIFFLL--LTLSWIPQRTFQQQPIKTVVVLVMENRSFDHMIGWMKKY 60

Query: 61  INPQINGVTGDECNPVSTNNSNPGTICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS 120
           I+P+INGVTGDECNPVST N NP TICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS
Sbjct: 61  IDPRINGVTGDECNPVSTKNPNPETICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS 120

Query: 121 GFVEQALSMSPNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA 180
           GFVEQALSMS NLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA
Sbjct: 121 GFVEQALSMSSNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA 180

Query: 181 TSHGSTSHVKKQLALGYPQKTIFDSLHENGHDFGIYFQNIPTTLFYRNLRKLKYIFKFHQ 240
           TSHGSTSHVKKQLALGYPQKTIFDSLHENG DFG+YFQNIPTTLFYRNLRKLKYIFKFH 
Sbjct: 181 TSHGSTSHVKKQLALGYPQKTIFDSLHENGLDFGVYFQNIPTTLFYRNLRKLKYIFKFHP 240

Query: 241 YDLKFKKDARNGKLPSLSVIEPRYFDLVGFPANDDHPSHDVANGQKLVKEVYETLRASPQ 300
           YDL+FKKDARNGKLPSL+VIEPRYFDLVG PANDDHPSHDVANGQKLVKEVYETLRASPQ
Sbjct: 241 YDLQFKKDARNGKLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQ 300

Query: 301 WNETLLIITYDEHGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK 360
           WN+TLLIITYDEHGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK
Sbjct: 301 WNQTLLIITYDEHGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK 360

Query: 361 KGTVISSPNGPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEHIVGQLTSPRTD 420
           KGTVISSP GPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFE IVGQLTSPRTD
Sbjct: 361 KGTVISSPKGPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTD 420

Query: 421 CPVTLPDVTPLRKTEANENSGLSEFQSEVVQLAAVLNGDHFLSTFPNEISKKMSVKEAHD 480
           CPVTLP+VTPLRKTEA ENSGLSEFQSEVVQLAAVLNGDHFLS+FPNEIS+KM+VKEAHD
Sbjct: 421 CPVTLPEVTPLRKTEAKENSGLSEFQSEVVQLAAVLNGDHFLSSFPNEISEKMTVKEAHD 480

Query: 481 YTRGAVARFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN 521
           YTRGAV+RFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN
Sbjct: 481 YTRGAVSRFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN 519

BLAST of MS001836 vs. ExPASy Swiss-Prot
Match: Q8H965 (Non-specific phospholipase C6 OS=Arabidopsis thaliana OX=3702 GN=NPC6 PE=2 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 1.1e-236
Identity = 395/485 (81.44%), Postives = 431/485 (88.87%), Query Frame = 0

Query: 33  QQPIKTVVVLVMENRSFDHMIGWMKKSINPQINGVTGDECNPVSTNNSNPGTICFSDDAE 92
           Q PIKTVVVLV+ENRSFDH++GWMK S+NP INGVTG ECNPV  +     TICF+ DAE
Sbjct: 36  QSPIKTVVVLVLENRSFDHLLGWMKNSVNPTINGVTGQECNPVPNSTQ---TICFTSDAE 95

Query: 93  FVDPDPGHSFEDVLQQVFGS--NSIPSMSGFVEQALSMSPNLSETVMKGFKPEAVPIYGA 152
           FVDPDPGHSFE V QQVFGS    IPSM GFVEQALSM  NLSETVMKGF+PEAVP+Y  
Sbjct: 96  FVDPDPGHSFEAVEQQVFGSGPGQIPSMMGFVEQALSMPGNLSETVMKGFRPEAVPVYAE 155

Query: 153 LVREFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQLALGYPQKTIFDSLHENGH 212
           LV+EFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQLA GYPQKTIFDSLH N  
Sbjct: 156 LVKEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQLAQGYPQKTIFDSLHSNDI 215

Query: 213 DFGIYFQNIPTTLFYRNLRKLKYIFKFHQYDLKFKKDARNGKLPSLSVIEPRYFDLVGFP 272
           DFGIYFQNIPTTLFYRNLR+LKYIF  HQYDLKFKKDA  GKLPSL+VIEPRYFDL G P
Sbjct: 216 DFGIYFQNIPTTLFYRNLRQLKYIFNLHQYDLKFKKDAAKGKLPSLTVIEPRYFDLKGLP 275

Query: 273 ANDDHPSHDVANGQKLVKEVYETLRASPQWNETLLIITYDEHGGFFDHVKTPFVNVPNPD 332
           ANDDHPSHDVANGQKLVKEVYE LR+SPQWNETLL+ITYDEHGGF+DHVKTP+V +PNPD
Sbjct: 276 ANDDHPSHDVANGQKLVKEVYEALRSSPQWNETLLVITYDEHGGFYDHVKTPYVGIPNPD 335

Query: 333 GNTGPAPYFFKFDRLGVRVPTIMVSPWIKKGTVISSPNGPTPNSEFEHSSIPATIKKIFN 392
           GNTGPAP FFKFDRLGVRVPTIMVSPWI+KGTV+S   GPT +SE+EHSSIPATIKK+FN
Sbjct: 336 GNTGPAPGFFKFDRLGVRVPTIMVSPWIQKGTVVSEAKGPTESSEYEHSSIPATIKKLFN 395

Query: 393 LSSNFLTHRDAWAGTFEHIVGQLTSPRTDCPVTLPDVTPLRKTEANENSGLSEFQSEVVQ 452
           LSSNFLTHRDAWA TFE +V  LT+PRTDCP+TLP+V P+R TE  E++ LSEFQ EVVQ
Sbjct: 396 LSSNFLTHRDAWAATFEDVVSHLTTPRTDCPMTLPEVAPMRATEPKEDAALSEFQGEVVQ 455

Query: 453 LAAVLNGDHFLSTFPNEISKKMSVKEAHDYTRGAVARFIRASKEAIKLGADESAIVDMRS 512
           LAAVLNGDHFLS+FP EI KKM+VK+AH+Y +GA +RFIRASKEA+KLGAD+SAIVDMRS
Sbjct: 456 LAAVLNGDHFLSSFPEEIGKKMTVKQAHEYVKGATSRFIRASKEAMKLGADKSAIVDMRS 515

Query: 513 SLTTR 516
           SLTTR
Sbjct: 516 SLTTR 517

BLAST of MS001836 vs. ExPASy Swiss-Prot
Match: Q8L7Y9 (Non-specific phospholipase C1 OS=Arabidopsis thaliana OX=3702 GN=NPC1 PE=2 SV=1)

HSP 1 Score: 622.1 bits (1603), Expect = 5.7e-177
Identity = 303/494 (61.34%), Postives = 377/494 (76.32%), Query Frame = 0

Query: 28  PQRICQQPIKTVVVLVMENRSFDHMIGWMKKSINPQINGVTGDECNPVSTNNSNPGTICF 87
           P +I Q PIKT+VV+VMENRSFDH++GW+ KS  P+I+G+TG E NP++ ++ N   I  
Sbjct: 31  PHKI-QGPIKTIVVVVMENRSFDHILGWL-KSTRPEIDGLTGKESNPLNVSDPNSKKIFV 90

Query: 88  SDDAEFVDPDPGHSFEDVLQQVFGSNSI---PSMSGFVEQALSMSPNLSETVMKGFKPEA 147
           SDDA FVD DPGHSF+ + +Q+FGSN     P M+GF +Q+ SM P +++ VM GFKPE 
Sbjct: 91  SDDAVFVDMDPGHSFQAIREQIFGSNDTSGDPKMNGFAQQSESMEPGMAKNVMSGFKPEV 150

Query: 148 VPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQLALGYPQKTIFDS 207
           +P+Y  L  EF VFDRWF+S+P  TQPNR +V+SATSHG +S+VKK L  G+PQKTIFDS
Sbjct: 151 LPVYTELANEFGVFDRWFASVPTSTQPNRFYVHSATSHGCSSNVKKDLVKGFPQKTIFDS 210

Query: 208 LHENGHDFGIYFQNIPTTLFYRNLRKLKYIFKFHQYDLKFKKDARNGKLPSLSVIEPRYF 267
           L ENG  FGIY+QNIP T F+++LR+LK++ KFH Y LKFK DA+ GKLP+ SV+E RYF
Sbjct: 211 LDENGLSFGIYYQNIPATFFFKSLRRLKHLVKFHSYALKFKLDAKLGKLPNYSVVEQRYF 270

Query: 268 DLVGFPANDDHPSHDVANGQKLVKEVYETLRASPQWNETLLIITYDEHGGFFDHVKTPFV 327
           D+  FPANDDHPSHDVA GQ+ VKEVYETLR+SPQW E  L+ITYDEHGGF+DHV TP  
Sbjct: 271 DIDLFPANDDHPSHDVAAGQRFVKEVYETLRSSPQWKEMALLITYDEHGGFYDHVPTPVK 330

Query: 328 NVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIKKGTVISSPNGPTPNSEFEHSSIPAT 387
            VPNPDG  GP P++F FDRLGVRVPT ++SPWI+KGTVI  P GPTP+S+FEHSSIPAT
Sbjct: 331 GVPNPDGIIGPDPFYFGFDRLGVRVPTFLISPWIEKGTVIHEPEGPTPHSQFEHSSIPAT 390

Query: 388 IKKIFNLSSNFLTHRDAWAGTFEHIVGQLTSPRTDCPVTLPDV-TPLRKTEANENSGLSE 447
           +KK+FNL S+FLT RDAWAGTFE       SPR DCP  LP+V   LR   A E+S LSE
Sbjct: 391 VKKLFNLKSHFLTKRDAWAGTFEKYFRIRDSPRQDCPEKLPEVKLSLRPWGAKEDSKLSE 450

Query: 448 FQSEVVQLAAVLNGDHFLSTFPNEISKKMSVKEAHDYTRGAVARFIRASKEAIKLGADES 507
           FQ E++QLA+ L GDH L+++P +I K M+V E + Y   AV +F+ A   A++ GADE+
Sbjct: 451 FQVELIQLASQLVGDHLLNSYP-DIGKNMTVSEGNKYAEDAVQKFLEAGMAALEAGADEN 510

Query: 508 AIVDMRSSLTTRSS 518
            IV MR SLTTR+S
Sbjct: 511 TIVTMRPSLTTRTS 521

BLAST of MS001836 vs. ExPASy Swiss-Prot
Match: O81020 (Non-specific phospholipase C2 OS=Arabidopsis thaliana OX=3702 GN=NPC2 PE=2 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 1.8e-175
Identity = 302/488 (61.89%), Postives = 374/488 (76.64%), Query Frame = 0

Query: 35  PIKTVVVLVMENRSFDHMIGWMKKSINPQINGVTGDECNPVSTNNSNPGTICFSDDAEFV 94
           PIKT+VV+VMENRSFDHM+GWMKK +NP+INGV G E NPVS ++ +   I F   + +V
Sbjct: 26  PIKTIVVVVMENRSFDHMLGWMKK-LNPEINGVDGSESNPVSVSDPSSRKIKFGSGSHYV 85

Query: 95  DPDPGHSFEDVLQQVFGSNSI----PSMSGFVEQALSMSP--NLSETVMKGFKPEAVPIY 154
           DPDPGHSF+ + +QVFGSN      P M+GFV+QA S  P  N+S +VM GF+P+ VP+Y
Sbjct: 86  DPDPGHSFQAIREQVFGSNDTSMDPPPMNGFVQQAYSEDPSGNMSASVMNGFEPDKVPVY 145

Query: 155 GALVREFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQLALGYPQKTIFDSLHEN 214
            +LV EFAVFDRWF+S+P  TQPNR+FV+S TS G+TS+    LA GYPQ+TIFD+L + 
Sbjct: 146 KSLVSEFAVFDRWFASVPSSTQPNRMFVHSGTSAGATSNNPISLAKGYPQRTIFDNLDDE 205

Query: 215 GHDFGIYFQNIPTTLFYRNLRKLKYIFKFHQYDLKFKKDARNGKLPSLSVIEPRYFDLVG 274
              FGIY+QNIP  LFY++LRKLKY+FKFH Y   FK  A+ GKLP+ +VIE RY D + 
Sbjct: 206 EFSFGIYYQNIPAVLFYQSLRKLKYVFKFHSYGNSFKDHAKQGKLPAYTVIEQRYMDTLL 265

Query: 275 FPANDDHPSHDVANGQKLVKEVYETLRASPQWNETLLIITYDEHGGFFDHVKTPFVNVPN 334
            PA+DDHPSHDV  GQK +KEVYETLRASPQWNETLLIITYDEHGG+FDHV TP  NVP+
Sbjct: 266 EPASDDHPSHDVYQGQKFIKEVYETLRASPQWNETLLIITYDEHGGYFDHVPTPVRNVPS 325

Query: 335 PDGNTGPAPYFFKFDRLGVRVPTIMVSPWIKKGTVISSPNG-PTPNSEFEHSSIPATIKK 394
           PDG  GP P+ F+F+RLG+RVPTI VSPWI+KGTV+  PNG P P+SE+EHSSIPAT+KK
Sbjct: 326 PDGIVGPDPFLFQFNRLGIRVPTIAVSPWIEKGTVVHGPNGSPFPSSEYEHSSIPATVKK 385

Query: 395 IFNLSSNFLTHRDAWAGTFEHIVGQLTSPRTDCPVTLPDVTPLRKTEANENSGLSEFQSE 454
           +FNLSS FLT RD WAGTFE+I+     PRTDCP TLP+   +R  EANE + L+EFQ E
Sbjct: 386 LFNLSSPFLTKRDEWAGTFENILQIRKEPRTDCPETLPEPVKIRMGEANEKALLTEFQQE 445

Query: 455 VVQLAAVLNGDHFLSTFPNEISKKMSVKEAHDYTRGAVARFIRASKEAIKLGADESAIVD 514
           +VQLAAVL GD+ L+TFP EISK M+V E   Y   A+ RF+ A + A+ +GA++  +V 
Sbjct: 446 LVQLAAVLKGDNMLTTFPKEISKGMTVIEGKRYMEDAMKRFLEAGRMALSMGANKEELVH 505

Query: 515 MRSSLTTR 516
           M++SLT R
Sbjct: 506 MKTSLTGR 512

BLAST of MS001836 vs. ExPASy Swiss-Prot
Match: Q9SRQ6 (Non-specific phospholipase C3 OS=Arabidopsis thaliana OX=3702 GN=NPC3 PE=1 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 1.1e-156
Identity = 271/496 (54.64%), Postives = 355/496 (71.57%), Query Frame = 0

Query: 35  PIKTVVVLVMENRSFDHMIGWMKKSINPQINGVTGDE--CNPVSTNNSNPGTICFSDDAE 94
           PIKT+VVLV ENRSFDHM+GW K+ +NP+I+GV+  E   NP+ST++ N   I F  +++
Sbjct: 15  PIKTIVVLVQENRSFDHMLGWFKE-LNPEIDGVSESEPRSNPLSTSDPNSAQIFFGKESQ 74

Query: 95  FVDPDPGHSFEDVLQQVFGS-------NSIPSMSGFVEQALSMSPNLSE-TVMKGFKPEA 154
            +DPDPGHSF+ + +QVFG           P M+GFV+ A +++  +SE  VM+GF PE 
Sbjct: 75  NIDPDPGHSFQAIYEQVFGKPFSDESPYPDPKMNGFVQNAEAITKGMSEKVVMQGFPPEK 134

Query: 155 VPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQLALGYPQKTIFDS 214
           +P++  LV+EFAV DRWFSS+P  TQPNRL+V++ATS+G+ S+    L  G+PQ+T+F+S
Sbjct: 135 LPVFKELVQEFAVCDRWFSSLPSSTQPNRLYVHAATSNGAFSNDTNTLVRGFPQRTVFES 194

Query: 215 LHENGHDFGIYFQNIPTTLFYRNLRKLKYIFKFHQYDLKFKKDARNGKLPSLSVIEPRYF 274
           L E+G  FGIY+Q+ P  LFYRN+RKLKY+  FHQY L FK+  + GKLP+  VIEPRYF
Sbjct: 195 LEESGFTFGIYYQSFPNCLFYRNMRKLKYVDNFHQYHLSFKRHCKEGKLPNYVVIEPRYF 254

Query: 275 DLVGFPANDDHPSHDVANGQKLVKEVYETLRASPQWNETLLIITYDEHGGFFDHVKTPFV 334
            ++  PANDDHP +DV  GQ LVKE+YE LRASPQWNE L ++ YDEHGG++DHV TP +
Sbjct: 255 KILSAPANDDHPKNDVVEGQNLVKEIYEALRASPQWNEILFVVVYDEHGGYYDHVPTPVI 314

Query: 335 NVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIKKGTVISSPNGPTPNSEFEHSSIPAT 394
            VPNPDG  GP PY FKFDRLGVRVP +++SPWI+ GTV+  PNGP P S+FEHSSIPAT
Sbjct: 315 GVPNPDGLVGPEPYNFKFDRLGVRVPALLISPWIEPGTVLHEPNGPEPTSQFEHSSIPAT 374

Query: 395 IKKIFNLSSNFLTHRDAWAGTFEHIVGQLTSPRTDCPVTLPDVTPLRKTE---ANENSGL 454
           +KKIFNL S FLT RD WAGT + ++ + TSPRTDCPVTLP++   R  +     E+  L
Sbjct: 375 LKKIFNLKS-FLTKRDEWAGTLDAVINR-TSPRTDCPVTLPELPRARDIDIGTQEEDEDL 434

Query: 455 SEFQSEVVQLAAVLNGDHFLSTFPNEISKKMSVKEAHDYTRGAVARFIRASKEAIKLGAD 514
           ++FQ E++Q AAVL GDH    +P +++ KM V +A  Y   A  RF   SK+A + G D
Sbjct: 435 TDFQIELIQAAAVLKGDHIKDIYPFKLADKMKVLDAARYVEEAFTRFHGESKKAKEEGRD 494

Query: 515 ESAIVDMRSSLTTRSS 518
           E  IVD+    T  S+
Sbjct: 495 EHEIVDLSKGSTRHST 507

BLAST of MS001836 vs. ExPASy Swiss-Prot
Match: Q9SRQ7 (Non-specific phospholipase C4 OS=Arabidopsis thaliana OX=3702 GN=NPC4 PE=1 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 6.8e-154
Identity = 271/484 (55.99%), Postives = 347/484 (71.69%), Query Frame = 0

Query: 35  PIKTVVVLVMENRSFDHMIGWMKKSINPQINGVTGDE--CNPVSTNNSNPGTICFSDDAE 94
           PIKT+VVLV ENRSFDH +GW K+ +N +I+GVT  +   N VS++++N   + F D ++
Sbjct: 13  PIKTIVVLVQENRSFDHTLGWFKE-LNREIDGVTKSDPKSNTVSSSDTNSLRVVFGDQSQ 72

Query: 95  FVDPDPGHSFEDVLQQVFG----------SNSIPSMSGFVEQALSMSPNLSETVMKGFKP 154
           +V+PDPGHS +D+ +QVFG          +   P+MSGF + A      +S  VM GFKP
Sbjct: 73  YVNPDPGHSIQDIYEQVFGKPWDSGKPDPNPGHPNMSGFAQNAERNKKGMSSAVMNGFKP 132

Query: 155 EAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQLALGYPQKTIF 214
            A+P+Y  LV+ FA+ DRWF+S+P  TQPNRL+V+SATSHG+TS+ KK L  G+PQKTIF
Sbjct: 133 NALPVYKELVQNFAICDRWFASVPASTQPNRLYVHSATSHGATSNDKKLLLEGFPQKTIF 192

Query: 215 DSLHENGHDFGIYFQNIPTTLFYRNLRKLKYIFKFHQYDLKFKKDARNGKLPSLSVIEPR 274
           +SL E G  FGIY+Q  P+TLFYRNLRKLKY+  FHQY ++FKKD + GKLP+  V+E R
Sbjct: 193 ESLDEAGFSFGIYYQFPPSTLFYRNLRKLKYLTHFHQYGIQFKKDCKEGKLPNYVVVEQR 252

Query: 275 YFDLVGFPANDDHPSHDVANGQKLVKEVYETLRASPQWNETLLIITYDEHGGFFDHVKTP 334
           +FDL+  PANDDHPSHDV+ GQKLVKEVYE LR+SPQWNE L IITYDEHGGF+DHV TP
Sbjct: 253 WFDLLSTPANDDHPSHDVSEGQKLVKEVYEALRSSPQWNEILFIITYDEHGGFYDHVPTP 312

Query: 335 FVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIKKGTVISSPNGPTPNSEFEHSSIP 394
              VPNPDG  GP PY F+F+RLGVRVPT  +SPWI+ GTVI  PNGP P S++EHSSIP
Sbjct: 313 VDGVPNPDGILGPPPYNFEFNRLGVRVPTFFISPWIEPGTVIHGPNGPYPRSQYEHSSIP 372

Query: 395 ATIKKIFNLSSNFLTHRDAWAGTFEHIVGQLTSPRTDCPVTLPDVTPLRKTEANENSGLS 454
           AT+K IF L  +FL+ RD+WAGTFE ++ +  SPR DCP TL     LR T A EN+ LS
Sbjct: 373 ATVKTIFKL-KDFLSKRDSWAGTFESVITR-DSPRQDCPETLSTPIKLRGTMAKENAQLS 432

Query: 455 EFQSEVVQLAAVLNGDHFLSTFPNEISKKMSVKEAHDYTRGAVARFIRASKEAIKLGADE 507
           EFQ ++V +AA L GD+      +++ K+  V +A  Y   A  +F+  S++A   G DE
Sbjct: 433 EFQEDLVIMAAGLKGDYKNEELIHKLCKETCVADASKYVTNAFEKFLEESRKARDRGCDE 492

BLAST of MS001836 vs. ExPASy TrEMBL
Match: A0A6J1BZ51 (non-specific phospholipase C6 OS=Momordica charantia OX=3673 GN=LOC111007060 PE=4 SV=1)

HSP 1 Score: 1068.9 bits (2763), Expect = 6.6e-309
Identity = 520/520 (100.00%), Postives = 520/520 (100.00%), Query Frame = 0

Query: 1   MRGRRNKPPFHLPFIFLFLFFLTLSWVPQRICQQPIKTVVVLVMENRSFDHMIGWMKKSI 60
           MRGRRNKPPFHLPFIFLFLFFLTLSWVPQRICQQPIKTVVVLVMENRSFDHMIGWMKKSI
Sbjct: 1   MRGRRNKPPFHLPFIFLFLFFLTLSWVPQRICQQPIKTVVVLVMENRSFDHMIGWMKKSI 60

Query: 61  NPQINGVTGDECNPVSTNNSNPGTICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMSG 120
           NPQINGVTGDECNPVSTNNSNPGTICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMSG
Sbjct: 61  NPQINGVTGDECNPVSTNNSNPGTICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMSG 120

Query: 121 FVEQALSMSPNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSAT 180
           FVEQALSMSPNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSAT
Sbjct: 121 FVEQALSMSPNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSAT 180

Query: 181 SHGSTSHVKKQLALGYPQKTIFDSLHENGHDFGIYFQNIPTTLFYRNLRKLKYIFKFHQY 240
           SHGSTSHVKKQLALGYPQKTIFDSLHENGHDFGIYFQNIPTTLFYRNLRKLKYIFKFHQY
Sbjct: 181 SHGSTSHVKKQLALGYPQKTIFDSLHENGHDFGIYFQNIPTTLFYRNLRKLKYIFKFHQY 240

Query: 241 DLKFKKDARNGKLPSLSVIEPRYFDLVGFPANDDHPSHDVANGQKLVKEVYETLRASPQW 300
           DLKFKKDARNGKLPSLSVIEPRYFDLVGFPANDDHPSHDVANGQKLVKEVYETLRASPQW
Sbjct: 241 DLKFKKDARNGKLPSLSVIEPRYFDLVGFPANDDHPSHDVANGQKLVKEVYETLRASPQW 300

Query: 301 NETLLIITYDEHGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIKK 360
           NETLLIITYDEHGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIKK
Sbjct: 301 NETLLIITYDEHGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIKK 360

Query: 361 GTVISSPNGPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEHIVGQLTSPRTDC 420
           GTVISSPNGPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEHIVGQLTSPRTDC
Sbjct: 361 GTVISSPNGPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEHIVGQLTSPRTDC 420

Query: 421 PVTLPDVTPLRKTEANENSGLSEFQSEVVQLAAVLNGDHFLSTFPNEISKKMSVKEAHDY 480
           PVTLPDVTPLRKTEANENSGLSEFQSEVVQLAAVLNGDHFLSTFPNEISKKMSVKEAHDY
Sbjct: 421 PVTLPDVTPLRKTEANENSGLSEFQSEVVQLAAVLNGDHFLSTFPNEISKKMSVKEAHDY 480

Query: 481 TRGAVARFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN 521
           TRGAVARFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN
Sbjct: 481 TRGAVARFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN 520

BLAST of MS001836 vs. ExPASy TrEMBL
Match: A0A0A0LRS0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G001490 PE=4 SV=1)

HSP 1 Score: 987.3 bits (2551), Expect = 2.5e-284
Identity = 485/521 (93.09%), Postives = 501/521 (96.16%), Query Frame = 0

Query: 1   MRGRRNKPPFHLPFIFLFLFFLTLSWVPQR-ICQQPIKTVVVLVMENRSFDHMIGWMKKS 60
           MRGR++K  FHLP IF  L  LTLSW+PQR   QQPIKTVVVLVMENRSFDHMIGWMKK 
Sbjct: 1   MRGRQSKFHFHLPSIFFLL--LTLSWLPQRSFQQQPIKTVVVLVMENRSFDHMIGWMKKY 60

Query: 61  INPQINGVTGDECNPVSTNNSNPGTICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS 120
           INPQINGVTGDECNPVST N NP TICF+DDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS
Sbjct: 61  INPQINGVTGDECNPVSTKNPNPETICFTDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS 120

Query: 121 GFVEQALSMSPNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA 180
           GFVEQALSMSPNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA
Sbjct: 121 GFVEQALSMSPNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA 180

Query: 181 TSHGSTSHVKKQLALGYPQKTIFDSLHENGHDFGIYFQNIPTTLFYRNLRKLKYIFKFHQ 240
           TSHGSTSHVKKQLA+GYPQKTIFDSLHENG +FGIYFQNIPTTLFYRNLRKLKYIFKFHQ
Sbjct: 181 TSHGSTSHVKKQLAMGYPQKTIFDSLHENGINFGIYFQNIPTTLFYRNLRKLKYIFKFHQ 240

Query: 241 YDLKFKKDARNGKLPSLSVIEPRYFDLVGFPANDDHPSHDVANGQKLVKEVYETLRASPQ 300
           YDLKFKKDARNGKLPSL+VIEPRYFDLVG PANDDHPSHDVANGQKLVKEVYETLRASPQ
Sbjct: 241 YDLKFKKDARNGKLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQ 300

Query: 301 WNETLLIITYDEHGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK 360
           WNETLLIITYDEHGGF+DHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK
Sbjct: 301 WNETLLIITYDEHGGFYDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK 360

Query: 361 KGTVISSPNGPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEHIVGQLTSPRTD 420
           KGTVISSP GPTPNSEFEHSSIPATIKKIFN+ SNFLTHRDAWAGTFE IV QLTSPRTD
Sbjct: 361 KGTVISSPKGPTPNSEFEHSSIPATIKKIFNIPSNFLTHRDAWAGTFEDIVDQLTSPRTD 420

Query: 421 CPVTLPDVTPLRKTEANENSGLSEFQSEVVQLAAVLNGDHFLSTFPNEISKKMSVKEAHD 480
           CPVTLP+VTPLRKTEANENSGLSEFQSEVVQLAAVLNGDHFLS+FPNEIS+KM++KEAHD
Sbjct: 421 CPVTLPEVTPLRKTEANENSGLSEFQSEVVQLAAVLNGDHFLSSFPNEISEKMTIKEAHD 480

Query: 481 YTRGAVARFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN 521
           YTRGAV+RFIRASKEAIKLGADESAIVDMRSSLTTRSS+HN
Sbjct: 481 YTRGAVSRFIRASKEAIKLGADESAIVDMRSSLTTRSSIHN 519

BLAST of MS001836 vs. ExPASy TrEMBL
Match: A0A6J1E0F1 (non-specific phospholipase C6-like OS=Cucurbita moschata OX=3662 GN=LOC111429704 PE=4 SV=1)

HSP 1 Score: 986.5 bits (2549), Expect = 4.3e-284
Identity = 485/521 (93.09%), Postives = 500/521 (95.97%), Query Frame = 0

Query: 1   MRGRRNKPPFHLPFIFLFLFFLTLSWVPQR-ICQQPIKTVVVLVMENRSFDHMIGWMKKS 60
           MRG R+K   HLPFIF  L  LTLSW+PQR   QQPIKTVVVLVMENRSFDHMIGWMKK 
Sbjct: 1   MRGGRSKVTVHLPFIFFLL--LTLSWIPQRTFQQQPIKTVVVLVMENRSFDHMIGWMKKY 60

Query: 61  INPQINGVTGDECNPVSTNNSNPGTICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS 120
           I+P+INGVTGDECNPVST N NP TICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS
Sbjct: 61  IDPRINGVTGDECNPVSTKNPNPETICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS 120

Query: 121 GFVEQALSMSPNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA 180
           GFVEQALSMS NLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA
Sbjct: 121 GFVEQALSMSSNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA 180

Query: 181 TSHGSTSHVKKQLALGYPQKTIFDSLHENGHDFGIYFQNIPTTLFYRNLRKLKYIFKFHQ 240
           TSHGSTSHVKKQLALGYPQKTIFDSLHENG DFG+YFQNIPTTLFYRNLRKLKYIFKFH 
Sbjct: 181 TSHGSTSHVKKQLALGYPQKTIFDSLHENGLDFGVYFQNIPTTLFYRNLRKLKYIFKFHP 240

Query: 241 YDLKFKKDARNGKLPSLSVIEPRYFDLVGFPANDDHPSHDVANGQKLVKEVYETLRASPQ 300
           YDL+FKKDARNGKLPSL+VIEPRYFDLVG PANDDHPSHDVANGQKLVKEVYETLRASPQ
Sbjct: 241 YDLQFKKDARNGKLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQ 300

Query: 301 WNETLLIITYDEHGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK 360
           WN+TLLIITYDEHGGFFDHV+TPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK
Sbjct: 301 WNQTLLIITYDEHGGFFDHVRTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK 360

Query: 361 KGTVISSPNGPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEHIVGQLTSPRTD 420
           KGTVISSP GPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFE IVGQLTSPRTD
Sbjct: 361 KGTVISSPKGPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTD 420

Query: 421 CPVTLPDVTPLRKTEANENSGLSEFQSEVVQLAAVLNGDHFLSTFPNEISKKMSVKEAHD 480
           CPVTLP+VTPLRKTEANENSG+SEFQSEVVQLAAVLNGDHFLS+FPNEIS+KM+VKEAHD
Sbjct: 421 CPVTLPEVTPLRKTEANENSGVSEFQSEVVQLAAVLNGDHFLSSFPNEISEKMTVKEAHD 480

Query: 481 YTRGAVARFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN 521
           YTRGAV+RFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN
Sbjct: 481 YTRGAVSRFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN 519

BLAST of MS001836 vs. ExPASy TrEMBL
Match: A0A6J1J9I6 (non-specific phospholipase C6-like OS=Cucurbita maxima OX=3661 GN=LOC111484775 PE=4 SV=1)

HSP 1 Score: 986.1 bits (2548), Expect = 5.6e-284
Identity = 486/521 (93.28%), Postives = 499/521 (95.78%), Query Frame = 0

Query: 1   MRGRRNKPPFHLPFIFLFLFFLTLSWVPQR-ICQQPIKTVVVLVMENRSFDHMIGWMKKS 60
           MR RR+K   HLPFIF  L  LTLSW+PQR   QQPIKTVVVLVMENRSFDHMIGWMKK 
Sbjct: 1   MRRRRSKVTVHLPFIFFLL--LTLSWIPQRTFQQQPIKTVVVLVMENRSFDHMIGWMKKY 60

Query: 61  INPQINGVTGDECNPVSTNNSNPGTICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS 120
           I+P+INGVTGDECNPVST N NP TICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS
Sbjct: 61  IDPRINGVTGDECNPVSTKNPNPETICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS 120

Query: 121 GFVEQALSMSPNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA 180
           GFVEQALSMS NLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA
Sbjct: 121 GFVEQALSMSSNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA 180

Query: 181 TSHGSTSHVKKQLALGYPQKTIFDSLHENGHDFGIYFQNIPTTLFYRNLRKLKYIFKFHQ 240
           TSHGSTSHVKKQLALGYPQKTIFDSLHENG DFG+YFQNIPTTLFYRNLRKLKYIFKFH 
Sbjct: 181 TSHGSTSHVKKQLALGYPQKTIFDSLHENGLDFGVYFQNIPTTLFYRNLRKLKYIFKFHP 240

Query: 241 YDLKFKKDARNGKLPSLSVIEPRYFDLVGFPANDDHPSHDVANGQKLVKEVYETLRASPQ 300
           YDL+FKKDARNGKLPSL+VIEPRYFDLVG PANDDHPSHDVANGQKLVKEVYETLRASPQ
Sbjct: 241 YDLQFKKDARNGKLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQ 300

Query: 301 WNETLLIITYDEHGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK 360
           WN+TLLIITYDEHGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK
Sbjct: 301 WNQTLLIITYDEHGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK 360

Query: 361 KGTVISSPNGPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEHIVGQLTSPRTD 420
           KGTVISSP GPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFE IVGQLTSPRTD
Sbjct: 361 KGTVISSPKGPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEDIVGQLTSPRTD 420

Query: 421 CPVTLPDVTPLRKTEANENSGLSEFQSEVVQLAAVLNGDHFLSTFPNEISKKMSVKEAHD 480
           CPVTLP+VTPLRKTEA ENSGLSEFQSEVVQLAAVLNGDHFLS+FPNEIS+KM+VKEAHD
Sbjct: 421 CPVTLPEVTPLRKTEAKENSGLSEFQSEVVQLAAVLNGDHFLSSFPNEISEKMTVKEAHD 480

Query: 481 YTRGAVARFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN 521
           YTRGAV+RFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN
Sbjct: 481 YTRGAVSRFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN 519

BLAST of MS001836 vs. ExPASy TrEMBL
Match: A0A5D3BH49 (Non-specific phospholipase C6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00480 PE=4 SV=1)

HSP 1 Score: 984.9 bits (2545), Expect = 1.2e-283
Identity = 484/521 (92.90%), Postives = 500/521 (95.97%), Query Frame = 0

Query: 1   MRGRRNKPPFHLPFIFLFLFFLTLSWVPQR-ICQQPIKTVVVLVMENRSFDHMIGWMKKS 60
           M GR++K PFHL  IF  L  LTLSW+PQR   QQPIKTVVVLVMENRSFDHMIGWMKK 
Sbjct: 1   MSGRQSKFPFHLSSIFFLL--LTLSWLPQRSFQQQPIKTVVVLVMENRSFDHMIGWMKKY 60

Query: 61  INPQINGVTGDECNPVSTNNSNPGTICFSDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS 120
           +NP+INGVTGDECNPVST N NP TICF+DDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS
Sbjct: 61  VNPKINGVTGDECNPVSTKNPNPETICFTDDAEFVDPDPGHSFEDVLQQVFGSNSIPSMS 120

Query: 121 GFVEQALSMSPNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA 180
           GFVEQALSMSPNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA
Sbjct: 121 GFVEQALSMSPNLSETVMKGFKPEAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSA 180

Query: 181 TSHGSTSHVKKQLALGYPQKTIFDSLHENGHDFGIYFQNIPTTLFYRNLRKLKYIFKFHQ 240
           TSHGSTSHVKKQLA+GYPQKTIFDSLHENG +FGIYFQNIPTTLFYRNLRKLKYIFKFHQ
Sbjct: 181 TSHGSTSHVKKQLAMGYPQKTIFDSLHENGINFGIYFQNIPTTLFYRNLRKLKYIFKFHQ 240

Query: 241 YDLKFKKDARNGKLPSLSVIEPRYFDLVGFPANDDHPSHDVANGQKLVKEVYETLRASPQ 300
           YDLKFKKDARNGKLPSL+VIEPRYFDLVG PANDDHPSHDVANGQKLVKEVYETLRASPQ
Sbjct: 241 YDLKFKKDARNGKLPSLTVIEPRYFDLVGMPANDDHPSHDVANGQKLVKEVYETLRASPQ 300

Query: 301 WNETLLIITYDEHGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK 360
           WNETLLIITYDEHGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK
Sbjct: 301 WNETLLIITYDEHGGFFDHVKTPFVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIK 360

Query: 361 KGTVISSPNGPTPNSEFEHSSIPATIKKIFNLSSNFLTHRDAWAGTFEHIVGQLTSPRTD 420
           KGTVISSP GPTPNSEFEHSSIPATIKKIFN+ SNFLTHRDAWAGTFE IV QLTSPRTD
Sbjct: 361 KGTVISSPKGPTPNSEFEHSSIPATIKKIFNIPSNFLTHRDAWAGTFEDIVDQLTSPRTD 420

Query: 421 CPVTLPDVTPLRKTEANENSGLSEFQSEVVQLAAVLNGDHFLSTFPNEISKKMSVKEAHD 480
           CPVTLP+VTPLRKTEANENSGLSEFQSEVVQLAAVLNGDHFLS+FPNEIS+KM+VKEAHD
Sbjct: 421 CPVTLPEVTPLRKTEANENSGLSEFQSEVVQLAAVLNGDHFLSSFPNEISEKMTVKEAHD 480

Query: 481 YTRGAVARFIRASKEAIKLGADESAIVDMRSSLTTRSSMHN 521
           YTRGAV+RFIRASKEAIKLGADESAIVDMRSSLTTRSS+HN
Sbjct: 481 YTRGAVSRFIRASKEAIKLGADESAIVDMRSSLTTRSSIHN 519

BLAST of MS001836 vs. TAIR 10
Match: AT3G48610.1 (non-specific phospholipase C6 )

HSP 1 Score: 820.5 bits (2118), Expect = 7.8e-238
Identity = 395/485 (81.44%), Postives = 431/485 (88.87%), Query Frame = 0

Query: 33  QQPIKTVVVLVMENRSFDHMIGWMKKSINPQINGVTGDECNPVSTNNSNPGTICFSDDAE 92
           Q PIKTVVVLV+ENRSFDH++GWMK S+NP INGVTG ECNPV  +     TICF+ DAE
Sbjct: 36  QSPIKTVVVLVLENRSFDHLLGWMKNSVNPTINGVTGQECNPVPNSTQ---TICFTSDAE 95

Query: 93  FVDPDPGHSFEDVLQQVFGS--NSIPSMSGFVEQALSMSPNLSETVMKGFKPEAVPIYGA 152
           FVDPDPGHSFE V QQVFGS    IPSM GFVEQALSM  NLSETVMKGF+PEAVP+Y  
Sbjct: 96  FVDPDPGHSFEAVEQQVFGSGPGQIPSMMGFVEQALSMPGNLSETVMKGFRPEAVPVYAE 155

Query: 153 LVREFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQLALGYPQKTIFDSLHENGH 212
           LV+EFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQLA GYPQKTIFDSLH N  
Sbjct: 156 LVKEFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQLAQGYPQKTIFDSLHSNDI 215

Query: 213 DFGIYFQNIPTTLFYRNLRKLKYIFKFHQYDLKFKKDARNGKLPSLSVIEPRYFDLVGFP 272
           DFGIYFQNIPTTLFYRNLR+LKYIF  HQYDLKFKKDA  GKLPSL+VIEPRYFDL G P
Sbjct: 216 DFGIYFQNIPTTLFYRNLRQLKYIFNLHQYDLKFKKDAAKGKLPSLTVIEPRYFDLKGLP 275

Query: 273 ANDDHPSHDVANGQKLVKEVYETLRASPQWNETLLIITYDEHGGFFDHVKTPFVNVPNPD 332
           ANDDHPSHDVANGQKLVKEVYE LR+SPQWNETLL+ITYDEHGGF+DHVKTP+V +PNPD
Sbjct: 276 ANDDHPSHDVANGQKLVKEVYEALRSSPQWNETLLVITYDEHGGFYDHVKTPYVGIPNPD 335

Query: 333 GNTGPAPYFFKFDRLGVRVPTIMVSPWIKKGTVISSPNGPTPNSEFEHSSIPATIKKIFN 392
           GNTGPAP FFKFDRLGVRVPTIMVSPWI+KGTV+S   GPT +SE+EHSSIPATIKK+FN
Sbjct: 336 GNTGPAPGFFKFDRLGVRVPTIMVSPWIQKGTVVSEAKGPTESSEYEHSSIPATIKKLFN 395

Query: 393 LSSNFLTHRDAWAGTFEHIVGQLTSPRTDCPVTLPDVTPLRKTEANENSGLSEFQSEVVQ 452
           LSSNFLTHRDAWA TFE +V  LT+PRTDCP+TLP+V P+R TE  E++ LSEFQ EVVQ
Sbjct: 396 LSSNFLTHRDAWAATFEDVVSHLTTPRTDCPMTLPEVAPMRATEPKEDAALSEFQGEVVQ 455

Query: 453 LAAVLNGDHFLSTFPNEISKKMSVKEAHDYTRGAVARFIRASKEAIKLGADESAIVDMRS 512
           LAAVLNGDHFLS+FP EI KKM+VK+AH+Y +GA +RFIRASKEA+KLGAD+SAIVDMRS
Sbjct: 456 LAAVLNGDHFLSSFPEEIGKKMTVKQAHEYVKGATSRFIRASKEAMKLGADKSAIVDMRS 515

Query: 513 SLTTR 516
           SLTTR
Sbjct: 516 SLTTR 517

BLAST of MS001836 vs. TAIR 10
Match: AT1G07230.1 (non-specific phospholipase C1 )

HSP 1 Score: 622.1 bits (1603), Expect = 4.1e-178
Identity = 303/494 (61.34%), Postives = 377/494 (76.32%), Query Frame = 0

Query: 28  PQRICQQPIKTVVVLVMENRSFDHMIGWMKKSINPQINGVTGDECNPVSTNNSNPGTICF 87
           P +I Q PIKT+VV+VMENRSFDH++GW+ KS  P+I+G+TG E NP++ ++ N   I  
Sbjct: 31  PHKI-QGPIKTIVVVVMENRSFDHILGWL-KSTRPEIDGLTGKESNPLNVSDPNSKKIFV 90

Query: 88  SDDAEFVDPDPGHSFEDVLQQVFGSNSI---PSMSGFVEQALSMSPNLSETVMKGFKPEA 147
           SDDA FVD DPGHSF+ + +Q+FGSN     P M+GF +Q+ SM P +++ VM GFKPE 
Sbjct: 91  SDDAVFVDMDPGHSFQAIREQIFGSNDTSGDPKMNGFAQQSESMEPGMAKNVMSGFKPEV 150

Query: 148 VPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQLALGYPQKTIFDS 207
           +P+Y  L  EF VFDRWF+S+P  TQPNR +V+SATSHG +S+VKK L  G+PQKTIFDS
Sbjct: 151 LPVYTELANEFGVFDRWFASVPTSTQPNRFYVHSATSHGCSSNVKKDLVKGFPQKTIFDS 210

Query: 208 LHENGHDFGIYFQNIPTTLFYRNLRKLKYIFKFHQYDLKFKKDARNGKLPSLSVIEPRYF 267
           L ENG  FGIY+QNIP T F+++LR+LK++ KFH Y LKFK DA+ GKLP+ SV+E RYF
Sbjct: 211 LDENGLSFGIYYQNIPATFFFKSLRRLKHLVKFHSYALKFKLDAKLGKLPNYSVVEQRYF 270

Query: 268 DLVGFPANDDHPSHDVANGQKLVKEVYETLRASPQWNETLLIITYDEHGGFFDHVKTPFV 327
           D+  FPANDDHPSHDVA GQ+ VKEVYETLR+SPQW E  L+ITYDEHGGF+DHV TP  
Sbjct: 271 DIDLFPANDDHPSHDVAAGQRFVKEVYETLRSSPQWKEMALLITYDEHGGFYDHVPTPVK 330

Query: 328 NVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIKKGTVISSPNGPTPNSEFEHSSIPAT 387
            VPNPDG  GP P++F FDRLGVRVPT ++SPWI+KGTVI  P GPTP+S+FEHSSIPAT
Sbjct: 331 GVPNPDGIIGPDPFYFGFDRLGVRVPTFLISPWIEKGTVIHEPEGPTPHSQFEHSSIPAT 390

Query: 388 IKKIFNLSSNFLTHRDAWAGTFEHIVGQLTSPRTDCPVTLPDV-TPLRKTEANENSGLSE 447
           +KK+FNL S+FLT RDAWAGTFE       SPR DCP  LP+V   LR   A E+S LSE
Sbjct: 391 VKKLFNLKSHFLTKRDAWAGTFEKYFRIRDSPRQDCPEKLPEVKLSLRPWGAKEDSKLSE 450

Query: 448 FQSEVVQLAAVLNGDHFLSTFPNEISKKMSVKEAHDYTRGAVARFIRASKEAIKLGADES 507
           FQ E++QLA+ L GDH L+++P +I K M+V E + Y   AV +F+ A   A++ GADE+
Sbjct: 451 FQVELIQLASQLVGDHLLNSYP-DIGKNMTVSEGNKYAEDAVQKFLEAGMAALEAGADEN 510

Query: 508 AIVDMRSSLTTRSS 518
            IV MR SLTTR+S
Sbjct: 511 TIVTMRPSLTTRTS 521

BLAST of MS001836 vs. TAIR 10
Match: AT2G26870.1 (non-specific phospholipase C2 )

HSP 1 Score: 617.1 bits (1590), Expect = 1.3e-176
Identity = 302/488 (61.89%), Postives = 374/488 (76.64%), Query Frame = 0

Query: 35  PIKTVVVLVMENRSFDHMIGWMKKSINPQINGVTGDECNPVSTNNSNPGTICFSDDAEFV 94
           PIKT+VV+VMENRSFDHM+GWMKK +NP+INGV G E NPVS ++ +   I F   + +V
Sbjct: 26  PIKTIVVVVMENRSFDHMLGWMKK-LNPEINGVDGSESNPVSVSDPSSRKIKFGSGSHYV 85

Query: 95  DPDPGHSFEDVLQQVFGSNSI----PSMSGFVEQALSMSP--NLSETVMKGFKPEAVPIY 154
           DPDPGHSF+ + +QVFGSN      P M+GFV+QA S  P  N+S +VM GF+P+ VP+Y
Sbjct: 86  DPDPGHSFQAIREQVFGSNDTSMDPPPMNGFVQQAYSEDPSGNMSASVMNGFEPDKVPVY 145

Query: 155 GALVREFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQLALGYPQKTIFDSLHEN 214
            +LV EFAVFDRWF+S+P  TQPNR+FV+S TS G+TS+    LA GYPQ+TIFD+L + 
Sbjct: 146 KSLVSEFAVFDRWFASVPSSTQPNRMFVHSGTSAGATSNNPISLAKGYPQRTIFDNLDDE 205

Query: 215 GHDFGIYFQNIPTTLFYRNLRKLKYIFKFHQYDLKFKKDARNGKLPSLSVIEPRYFDLVG 274
              FGIY+QNIP  LFY++LRKLKY+FKFH Y   FK  A+ GKLP+ +VIE RY D + 
Sbjct: 206 EFSFGIYYQNIPAVLFYQSLRKLKYVFKFHSYGNSFKDHAKQGKLPAYTVIEQRYMDTLL 265

Query: 275 FPANDDHPSHDVANGQKLVKEVYETLRASPQWNETLLIITYDEHGGFFDHVKTPFVNVPN 334
            PA+DDHPSHDV  GQK +KEVYETLRASPQWNETLLIITYDEHGG+FDHV TP  NVP+
Sbjct: 266 EPASDDHPSHDVYQGQKFIKEVYETLRASPQWNETLLIITYDEHGGYFDHVPTPVRNVPS 325

Query: 335 PDGNTGPAPYFFKFDRLGVRVPTIMVSPWIKKGTVISSPNG-PTPNSEFEHSSIPATIKK 394
           PDG  GP P+ F+F+RLG+RVPTI VSPWI+KGTV+  PNG P P+SE+EHSSIPAT+KK
Sbjct: 326 PDGIVGPDPFLFQFNRLGIRVPTIAVSPWIEKGTVVHGPNGSPFPSSEYEHSSIPATVKK 385

Query: 395 IFNLSSNFLTHRDAWAGTFEHIVGQLTSPRTDCPVTLPDVTPLRKTEANENSGLSEFQSE 454
           +FNLSS FLT RD WAGTFE+I+     PRTDCP TLP+   +R  EANE + L+EFQ E
Sbjct: 386 LFNLSSPFLTKRDEWAGTFENILQIRKEPRTDCPETLPEPVKIRMGEANEKALLTEFQQE 445

Query: 455 VVQLAAVLNGDHFLSTFPNEISKKMSVKEAHDYTRGAVARFIRASKEAIKLGADESAIVD 514
           +VQLAAVL GD+ L+TFP EISK M+V E   Y   A+ RF+ A + A+ +GA++  +V 
Sbjct: 446 LVQLAAVLKGDNMLTTFPKEISKGMTVIEGKRYMEDAMKRFLEAGRMALSMGANKEELVH 505

Query: 515 MRSSLTTR 516
           M++SLT R
Sbjct: 506 MKTSLTGR 512

BLAST of MS001836 vs. TAIR 10
Match: AT3G03520.1 (non-specific phospholipase C3 )

HSP 1 Score: 554.7 bits (1428), Expect = 8.0e-158
Identity = 271/496 (54.64%), Postives = 355/496 (71.57%), Query Frame = 0

Query: 35  PIKTVVVLVMENRSFDHMIGWMKKSINPQINGVTGDE--CNPVSTNNSNPGTICFSDDAE 94
           PIKT+VVLV ENRSFDHM+GW K+ +NP+I+GV+  E   NP+ST++ N   I F  +++
Sbjct: 15  PIKTIVVLVQENRSFDHMLGWFKE-LNPEIDGVSESEPRSNPLSTSDPNSAQIFFGKESQ 74

Query: 95  FVDPDPGHSFEDVLQQVFGS-------NSIPSMSGFVEQALSMSPNLSE-TVMKGFKPEA 154
            +DPDPGHSF+ + +QVFG           P M+GFV+ A +++  +SE  VM+GF PE 
Sbjct: 75  NIDPDPGHSFQAIYEQVFGKPFSDESPYPDPKMNGFVQNAEAITKGMSEKVVMQGFPPEK 134

Query: 155 VPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQLALGYPQKTIFDS 214
           +P++  LV+EFAV DRWFSS+P  TQPNRL+V++ATS+G+ S+    L  G+PQ+T+F+S
Sbjct: 135 LPVFKELVQEFAVCDRWFSSLPSSTQPNRLYVHAATSNGAFSNDTNTLVRGFPQRTVFES 194

Query: 215 LHENGHDFGIYFQNIPTTLFYRNLRKLKYIFKFHQYDLKFKKDARNGKLPSLSVIEPRYF 274
           L E+G  FGIY+Q+ P  LFYRN+RKLKY+  FHQY L FK+  + GKLP+  VIEPRYF
Sbjct: 195 LEESGFTFGIYYQSFPNCLFYRNMRKLKYVDNFHQYHLSFKRHCKEGKLPNYVVIEPRYF 254

Query: 275 DLVGFPANDDHPSHDVANGQKLVKEVYETLRASPQWNETLLIITYDEHGGFFDHVKTPFV 334
            ++  PANDDHP +DV  GQ LVKE+YE LRASPQWNE L ++ YDEHGG++DHV TP +
Sbjct: 255 KILSAPANDDHPKNDVVEGQNLVKEIYEALRASPQWNEILFVVVYDEHGGYYDHVPTPVI 314

Query: 335 NVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIKKGTVISSPNGPTPNSEFEHSSIPAT 394
            VPNPDG  GP PY FKFDRLGVRVP +++SPWI+ GTV+  PNGP P S+FEHSSIPAT
Sbjct: 315 GVPNPDGLVGPEPYNFKFDRLGVRVPALLISPWIEPGTVLHEPNGPEPTSQFEHSSIPAT 374

Query: 395 IKKIFNLSSNFLTHRDAWAGTFEHIVGQLTSPRTDCPVTLPDVTPLRKTE---ANENSGL 454
           +KKIFNL S FLT RD WAGT + ++ + TSPRTDCPVTLP++   R  +     E+  L
Sbjct: 375 LKKIFNLKS-FLTKRDEWAGTLDAVINR-TSPRTDCPVTLPELPRARDIDIGTQEEDEDL 434

Query: 455 SEFQSEVVQLAAVLNGDHFLSTFPNEISKKMSVKEAHDYTRGAVARFIRASKEAIKLGAD 514
           ++FQ E++Q AAVL GDH    +P +++ KM V +A  Y   A  RF   SK+A + G D
Sbjct: 435 TDFQIELIQAAAVLKGDHIKDIYPFKLADKMKVLDAARYVEEAFTRFHGESKKAKEEGRD 494

Query: 515 ESAIVDMRSSLTTRSS 518
           E  IVD+    T  S+
Sbjct: 495 EHEIVDLSKGSTRHST 507

BLAST of MS001836 vs. TAIR 10
Match: AT3G03530.1 (non-specific phospholipase C4 )

HSP 1 Score: 545.4 bits (1404), Expect = 4.9e-155
Identity = 271/484 (55.99%), Postives = 347/484 (71.69%), Query Frame = 0

Query: 35  PIKTVVVLVMENRSFDHMIGWMKKSINPQINGVTGDE--CNPVSTNNSNPGTICFSDDAE 94
           PIKT+VVLV ENRSFDH +GW K+ +N +I+GVT  +   N VS++++N   + F D ++
Sbjct: 13  PIKTIVVLVQENRSFDHTLGWFKE-LNREIDGVTKSDPKSNTVSSSDTNSLRVVFGDQSQ 72

Query: 95  FVDPDPGHSFEDVLQQVFG----------SNSIPSMSGFVEQALSMSPNLSETVMKGFKP 154
           +V+PDPGHS +D+ +QVFG          +   P+MSGF + A      +S  VM GFKP
Sbjct: 73  YVNPDPGHSIQDIYEQVFGKPWDSGKPDPNPGHPNMSGFAQNAERNKKGMSSAVMNGFKP 132

Query: 155 EAVPIYGALVREFAVFDRWFSSIPGPTQPNRLFVYSATSHGSTSHVKKQLALGYPQKTIF 214
            A+P+Y  LV+ FA+ DRWF+S+P  TQPNRL+V+SATSHG+TS+ KK L  G+PQKTIF
Sbjct: 133 NALPVYKELVQNFAICDRWFASVPASTQPNRLYVHSATSHGATSNDKKLLLEGFPQKTIF 192

Query: 215 DSLHENGHDFGIYFQNIPTTLFYRNLRKLKYIFKFHQYDLKFKKDARNGKLPSLSVIEPR 274
           +SL E G  FGIY+Q  P+TLFYRNLRKLKY+  FHQY ++FKKD + GKLP+  V+E R
Sbjct: 193 ESLDEAGFSFGIYYQFPPSTLFYRNLRKLKYLTHFHQYGIQFKKDCKEGKLPNYVVVEQR 252

Query: 275 YFDLVGFPANDDHPSHDVANGQKLVKEVYETLRASPQWNETLLIITYDEHGGFFDHVKTP 334
           +FDL+  PANDDHPSHDV+ GQKLVKEVYE LR+SPQWNE L IITYDEHGGF+DHV TP
Sbjct: 253 WFDLLSTPANDDHPSHDVSEGQKLVKEVYEALRSSPQWNEILFIITYDEHGGFYDHVPTP 312

Query: 335 FVNVPNPDGNTGPAPYFFKFDRLGVRVPTIMVSPWIKKGTVISSPNGPTPNSEFEHSSIP 394
              VPNPDG  GP PY F+F+RLGVRVPT  +SPWI+ GTVI  PNGP P S++EHSSIP
Sbjct: 313 VDGVPNPDGILGPPPYNFEFNRLGVRVPTFFISPWIEPGTVIHGPNGPYPRSQYEHSSIP 372

Query: 395 ATIKKIFNLSSNFLTHRDAWAGTFEHIVGQLTSPRTDCPVTLPDVTPLRKTEANENSGLS 454
           AT+K IF L  +FL+ RD+WAGTFE ++ +  SPR DCP TL     LR T A EN+ LS
Sbjct: 373 ATVKTIFKL-KDFLSKRDSWAGTFESVITR-DSPRQDCPETLSTPIKLRGTMAKENAQLS 432

Query: 455 EFQSEVVQLAAVLNGDHFLSTFPNEISKKMSVKEAHDYTRGAVARFIRASKEAIKLGADE 507
           EFQ ++V +AA L GD+      +++ K+  V +A  Y   A  +F+  S++A   G DE
Sbjct: 433 EFQEDLVIMAAGLKGDYKNEELIHKLCKETCVADASKYVTNAFEKFLEESRKARDRGCDE 492