MS001813 (gene) Bitter gourd (TR) v1

Overview
NameMS001813
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionMitochondrial import inner membrane translocase subunit
Locationscaffold30: 196951 .. 197134 (+)
RNA-Seq ExpressionMS001813
SyntenyMS001813
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGAGAAGCAAAGAGCTATGGTTGCTGAGATGGTGGCAAAGCTTACCAGTGTGTGCTGGGACAAAGTGTATCACTGGTTCGCCAGGGAGTAAGTTCAGCTCCAGGGAATCCAACTGTCTATCAAACTGCGCCCAACGCTATATGGATATGAGCATCGTCATTATGAAACGGTTCCAAAACAGT

mRNA sequence

GAAGAGAAGCAAAGAGCTATGGTTGCTGAGATGGTGGCAAAGCTTACCAGTGTGTGCTGGGACTGTATCACTGGTTCGCCAGGGAGTAAGTTCAGCTCCAGGGAATCCAACTGTCTATCAAACTGCGCCCAACGCTATATGGATATGAGCATCGTCATTATGAAACGGTTCCAAAACAGT

Coding sequence (CDS)

GAAGAGAAGCAAAGAGCTATGGTTGCTGAGATGGTGGCAAAGCTTACCAGTGTGTGCTGGGACTGTATCACTGGTTCGCCAGGGAGTAAGTTCAGCTCCAGGGAATCCAACTGTCTATCAAACTGCGCCCAACGCTATATGGATATGAGCATCGTCATTATGAAACGGTTCCAAAACAGT

Protein sequence

EEKQRAMVAEMVAKLTSVCWDCITGSPGSKFSSRESNCLSNCAQRYMDMSIVIMKRFQNS
Homology
BLAST of MS001813 vs. NCBI nr
Match: XP_022135207.1 (mitochondrial import inner membrane translocase subunit TIM8 [Momordica charantia])

HSP 1 Score: 116.7 bits (291), Expect = 6.9e-23
Identity = 58/61 (95.08%), Postives = 59/61 (96.72%), Query Frame = 0

Query: 1  EEKQRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIVIMKRFQN 60
          EEKQRAMVAEMVAKLTSVCWD CITGSPGSKFSS ESNCLSNCAQRYMDMSI+IMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

BLAST of MS001813 vs. NCBI nr
Match: XP_008464391.1 (PREDICTED: mitochondrial import inner membrane translocase subunit TIM8 [Cucumis melo] >XP_011649996.1 mitochondrial import inner membrane translocase subunit TIM8 [Cucumis sativus] >XP_022921432.1 mitochondrial import inner membrane translocase subunit TIM8 [Cucurbita moschata] >XP_022924732.1 mitochondrial import inner membrane translocase subunit TIM8-like [Cucurbita moschata] >XP_022974369.1 mitochondrial import inner membrane translocase subunit TIM8-like [Cucurbita maxima] >XP_022988609.1 mitochondrial import inner membrane translocase subunit TIM8 [Cucurbita maxima] >XP_023516926.1 mitochondrial import inner membrane translocase subunit TIM8 [Cucurbita pepo subsp. pepo] >XP_023529319.1 mitochondrial import inner membrane translocase subunit TIM8-like [Cucurbita pepo subsp. pepo] >XP_038878330.1 mitochondrial import inner membrane translocase subunit TIM8 [Benincasa hispida] >KGN63456.1 hypothetical protein Csa_014027 [Cucumis sativus])

HSP 1 Score: 115.5 bits (288), Expect = 1.5e-22
Identity = 57/61 (93.44%), Postives = 59/61 (96.72%), Query Frame = 0

Query: 1  EEKQRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIVIMKRFQN 60
          EEKQRAMVAEMVAKLTSVCWD CITG+PGSKFSS ESNCLSNCAQRYMDMSI+IMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWDKCITGTPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

BLAST of MS001813 vs. NCBI nr
Match: KAG7023044.1 (Mitochondrial import inner membrane translocase subunit TIM8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 110.9 bits (276), Expect = 3.8e-21
Identity = 54/60 (90.00%), Postives = 57/60 (95.00%), Query Frame = 0

Query: 1  EEKQRAMVAEMVAKLTSVCW-DCITGSPGSKFSSRESNCLSNCAQRYMDMSIVIMKRFQN 60
          EEKQRAMVAEMVAKLTSVCW  C+TG+PGSKFSS ESNCLSNCAQRYMDMSI+IMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWTKCVTGTPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

BLAST of MS001813 vs. NCBI nr
Match: XP_028764582.1 (mitochondrial import inner membrane translocase subunit TIM8-like [Prosopis alba] >XP_028765220.1 mitochondrial import inner membrane translocase subunit TIM8-like [Prosopis alba])

HSP 1 Score: 103.6 bits (257), Expect = 6.0e-19
Identity = 50/60 (83.33%), Postives = 55/60 (91.67%), Query Frame = 0

Query: 1  EEKQRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIVIMKRFQN 60
          +EK+RAMV EMVAKLTS CWD CITG+PGSKFSS ES CLSNCAQRYMD+SI+IMKRFQN
Sbjct: 17 QEKERAMVNEMVAKLTSACWDKCITGTPGSKFSSSESTCLSNCAQRYMDLSILIMKRFQN 76

BLAST of MS001813 vs. NCBI nr
Match: XP_010250594.1 (PREDICTED: mitochondrial import inner membrane translocase subunit TIM8 [Nelumbo nucifera])

HSP 1 Score: 103.2 bits (256), Expect = 7.9e-19
Identity = 49/60 (81.67%), Postives = 56/60 (93.33%), Query Frame = 0

Query: 1  EEKQRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIVIMKRFQN 60
          +EKQ+AMV EMVAKLT+VCWD CITG+PGSKFSS ES CLSNCAQRYMDMS++IMKRFQ+
Sbjct: 17 QEKQKAMVNEMVAKLTNVCWDKCITGTPGSKFSSSESTCLSNCAQRYMDMSLIIMKRFQS 76

BLAST of MS001813 vs. ExPASy Swiss-Prot
Match: Q9XGY4 (Mitochondrial import inner membrane translocase subunit TIM8 OS=Arabidopsis thaliana OX=3702 GN=TIM8 PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 2.8e-19
Identity = 44/60 (73.33%), Postives = 55/60 (91.67%), Query Frame = 0

Query: 1  EEKQRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIVIMKRFQN 60
          +EK+RAMV EMV+K+TSVCWD CIT +PGSKFSS ES+CL++CAQRYMDMS++IMKRF +
Sbjct: 17 QEKERAMVNEMVSKMTSVCWDKCITSAPGSKFSSSESSCLTHCAQRYMDMSMIIMKRFNS 76

BLAST of MS001813 vs. ExPASy Swiss-Prot
Match: P0CR95 (Mitochondrial import inner membrane translocase subunit TIM8 OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=TIM8 PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.2e-07
Identity = 23/59 (38.98%), Postives = 39/59 (66.10%), Query Frame = 0

Query: 1  EEKQRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIVIMKRFQ 59
          +E+ +A +   + +LT+ CW+ CITG   SKFS  E+ CL NC  R++D S+ I+++ +
Sbjct: 24 QEQAKAKLQASIHELTNTCWNTCITGGISSKFSKSEAQCLENCVDRFLDSSLYIVRQIE 82

BLAST of MS001813 vs. ExPASy Swiss-Prot
Match: P0CR94 (Mitochondrial import inner membrane translocase subunit TIM8 OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) OX=214684 GN=TIM8 PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.2e-07
Identity = 23/59 (38.98%), Postives = 39/59 (66.10%), Query Frame = 0

Query: 1  EEKQRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIVIMKRFQ 59
          +E+ +A +   + +LT+ CW+ CITG   SKFS  E+ CL NC  R++D S+ I+++ +
Sbjct: 24 QEQAKAKLQASIHELTNTCWNTCITGGISSKFSKSEAQCLENCVDRFLDSSLYIVRQIE 82

BLAST of MS001813 vs. ExPASy Swiss-Prot
Match: Q9Y1A3 (Mitochondrial import inner membrane translocase subunit Tim8 OS=Drosophila melanogaster OX=7227 GN=Tim8 PE=3 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 1.2e-06
Identity = 21/56 (37.50%), Postives = 32/56 (57.14%), Query Frame = 0

Query: 2  EKQRAMVAEMVAKLTSVCWDCITGSPGSKFSSRESNCLSNCAQRYMDMSIVIMKRF 58
          EKQ+A V   + +   +CW+   G P +K       CLSNC  R++D S++I +RF
Sbjct: 21 EKQKAQVNAQIHEFNEICWEKCIGKPSTKLDHATETCLSNCVDRFIDTSLLITQRF 76

BLAST of MS001813 vs. ExPASy Swiss-Prot
Match: P62077 (Mitochondrial import inner membrane translocase subunit Tim8 B OS=Mus musculus OX=10090 GN=Timm8b PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 2.7e-06
Identity = 22/56 (39.29%), Postives = 31/56 (55.36%), Query Frame = 0

Query: 2  EKQRAMVAEMVAKLTSVCWDCITGSPGSKFSSRESNCLSNCAQRYMDMSIVIMKRF 58
          E+Q+A     V     +CWD     PGS+  SR  NCLS+C  R++D ++ I  RF
Sbjct: 19 EQQKAQFTAQVHHFMELCWDKCVEKPGSRLDSRTENCLSSCVDRFIDTTLAITGRF 74

BLAST of MS001813 vs. ExPASy TrEMBL
Match: A0A6J1C212 (Mitochondrial import inner membrane translocase subunit OS=Momordica charantia OX=3673 GN=LOC111007226 PE=3 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 3.3e-23
Identity = 58/61 (95.08%), Postives = 59/61 (96.72%), Query Frame = 0

Query: 1  EEKQRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIVIMKRFQN 60
          EEKQRAMVAEMVAKLTSVCWD CITGSPGSKFSS ESNCLSNCAQRYMDMSI+IMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

BLAST of MS001813 vs. ExPASy TrEMBL
Match: A0A6J1IHF0 (Mitochondrial import inner membrane translocase subunit OS=Cucurbita maxima OX=3661 GN=LOC111472993 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 7.4e-23
Identity = 57/61 (93.44%), Postives = 59/61 (96.72%), Query Frame = 0

Query: 1  EEKQRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIVIMKRFQN 60
          EEKQRAMVAEMVAKLTSVCWD CITG+PGSKFSS ESNCLSNCAQRYMDMSI+IMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWDKCITGTPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

BLAST of MS001813 vs. ExPASy TrEMBL
Match: A0A6J1E0G1 (Mitochondrial import inner membrane translocase subunit OS=Cucurbita moschata OX=3662 GN=LOC111429714 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 7.4e-23
Identity = 57/61 (93.44%), Postives = 59/61 (96.72%), Query Frame = 0

Query: 1  EEKQRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIVIMKRFQN 60
          EEKQRAMVAEMVAKLTSVCWD CITG+PGSKFSS ESNCLSNCAQRYMDMSI+IMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWDKCITGTPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

BLAST of MS001813 vs. ExPASy TrEMBL
Match: A0A0A0LTY7 (Mitochondrial import inner membrane translocase subunit OS=Cucumis sativus OX=3659 GN=Csa_1G001280 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 7.4e-23
Identity = 57/61 (93.44%), Postives = 59/61 (96.72%), Query Frame = 0

Query: 1  EEKQRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIVIMKRFQN 60
          EEKQRAMVAEMVAKLTSVCWD CITG+PGSKFSS ESNCLSNCAQRYMDMSI+IMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWDKCITGTPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

BLAST of MS001813 vs. ExPASy TrEMBL
Match: A0A1S3CLD0 (Mitochondrial import inner membrane translocase subunit OS=Cucumis melo OX=3656 GN=LOC103502294 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 7.4e-23
Identity = 57/61 (93.44%), Postives = 59/61 (96.72%), Query Frame = 0

Query: 1  EEKQRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIVIMKRFQN 60
          EEKQRAMVAEMVAKLTSVCWD CITG+PGSKFSS ESNCLSNCAQRYMDMSI+IMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWDKCITGTPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

BLAST of MS001813 vs. TAIR 10
Match: AT5G50810.1 (translocase inner membrane subunit 8 )

HSP 1 Score: 95.1 bits (235), Expect = 2.0e-20
Identity = 44/60 (73.33%), Postives = 55/60 (91.67%), Query Frame = 0

Query: 1  EEKQRAMVAEMVAKLTSVCWD-CITGSPGSKFSSRESNCLSNCAQRYMDMSIVIMKRFQN 60
          +EK+RAMV EMV+K+TSVCWD CIT +PGSKFSS ES+CL++CAQRYMDMS++IMKRF +
Sbjct: 17 QEKERAMVNEMVSKMTSVCWDKCITSAPGSKFSSSESSCLTHCAQRYMDMSMIIMKRFNS 76

BLAST of MS001813 vs. TAIR 10
Match: AT1G61570.1 (translocase of the inner mitochondrial membrane 13 )

HSP 1 Score: 42.7 bits (99), Expect = 1.2e-04
Identity = 17/51 (33.33%), Postives = 29/51 (56.86%), Query Frame = 0

Query: 5  RAMVAEMVAKLTSVCWDCITGSPGSKFSSRESNCLSNCAQRYMDMSIVIMK 56
          +A   E++  L + C+D     PGS     ES+C+S C +RYM+ + +I +
Sbjct: 31 QAYAEELIETLRTKCFDKCVTKPGSSLGGSESSCISRCVERYMEATAIISR 81

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135207.16.9e-2395.08mitochondrial import inner membrane translocase subunit TIM8 [Momordica charanti... [more]
XP_008464391.11.5e-2293.44PREDICTED: mitochondrial import inner membrane translocase subunit TIM8 [Cucumis... [more]
KAG7023044.13.8e-2190.00Mitochondrial import inner membrane translocase subunit TIM8, partial [Cucurbita... [more]
XP_028764582.16.0e-1983.33mitochondrial import inner membrane translocase subunit TIM8-like [Prosopis alba... [more]
XP_010250594.17.9e-1981.67PREDICTED: mitochondrial import inner membrane translocase subunit TIM8 [Nelumbo... [more]
Match NameE-valueIdentityDescription
Q9XGY42.8e-1973.33Mitochondrial import inner membrane translocase subunit TIM8 OS=Arabidopsis thal... [more]
P0CR953.2e-0738.98Mitochondrial import inner membrane translocase subunit TIM8 OS=Cryptococcus neo... [more]
P0CR943.2e-0738.98Mitochondrial import inner membrane translocase subunit TIM8 OS=Cryptococcus neo... [more]
Q9Y1A31.2e-0637.50Mitochondrial import inner membrane translocase subunit Tim8 OS=Drosophila melan... [more]
P620772.7e-0639.29Mitochondrial import inner membrane translocase subunit Tim8 B OS=Mus musculus O... [more]
Match NameE-valueIdentityDescription
A0A6J1C2123.3e-2395.08Mitochondrial import inner membrane translocase subunit OS=Momordica charantia O... [more]
A0A6J1IHF07.4e-2393.44Mitochondrial import inner membrane translocase subunit OS=Cucurbita maxima OX=3... [more]
A0A6J1E0G17.4e-2393.44Mitochondrial import inner membrane translocase subunit OS=Cucurbita moschata OX... [more]
A0A0A0LTY77.4e-2393.44Mitochondrial import inner membrane translocase subunit OS=Cucumis sativus OX=36... [more]
A0A1S3CLD07.4e-2393.44Mitochondrial import inner membrane translocase subunit OS=Cucumis melo OX=3656 ... [more]
Match NameE-valueIdentityDescription
AT5G50810.12.0e-2073.33translocase inner membrane subunit 8 [more]
AT1G61570.11.2e-0433.33translocase of the inner mitochondrial membrane 13 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004217Tim10-likePFAMPF02953zf-Tim10_DDPcoord: 1..59
e-value: 3.9E-20
score: 71.2
IPR035427Tim10-like domain superfamilyGENE3D1.10.287.810Mitochondrial import inner membrane translocase subunit tim13 like domainscoord: 1..60
e-value: 3.0E-19
score: 70.2
IPR035427Tim10-like domain superfamilySUPERFAMILY144122Tim10-likecoord: 4..59
NoneNo IPR availablePANTHERPTHR19338:SF15MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM8-LIKEcoord: 2..59
IPR039238Mitochondrial import inner membrane translocase subunit Tim8/13PANTHERPTHR19338TRANSLOCASE OF INNER MITOCHONDRIAL MEMBRANE 13 HOMOLOGcoord: 2..59

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS001813.1MS001813.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005758 mitochondrial intermembrane space