MS001812 (gene) Bitter gourd (TR) v1

Overview


Name	MS001812
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Mitochondrial import inner membrane translocase subunit
Location	scaffold30: 194264 .. 194446 (+)
RNA-Seq Expression	MS001812
Synteny	MS001812

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAGAGAAGCAAAGAGCTATGGTTGCTGAGATGGTGGCAAAGCTCACCAGTGTGTGTTGGGACAAGTGTATCACTGGTTCGCCTGGGAGTAAGTTCAGCTCCAGCGAATCCAACTGTCTATCAAACTGCGCTCAACGCTATATGGATATGAGCATCATCATTATGAAACGGTTCCAAAACAGT

mRNA sequence

GAAGAGAAGCAAAGAGCTATGGTTGCTGAGATGGTGGCAAAGCTCACCAGTGTGTGTTGGGACAAGTGTATCACTGGTTCGCCTGGGAGTAAGTTCAGCTCCAGCGAATCCAACTGTCTATCAAACTGCGCTCAACGCTATATGGATATGAGCATCATCATTATGAAACGGTTCCAAAACAGT

Coding sequence (CDS)

GAAGAGAAGCAAAGAGCTATGGTTGCTGAGATGGTGGCAAAGCTCACCAGTGTGTGTTGGGACAAGTGTATCACTGGTTCGCCTGGGAGTAAGTTCAGCTCCAGCGAATCCAACTGTCTATCAAACTGCGCTCAACGCTATATGGATATGAGCATCATCATTATGAAACGGTTCCAAAACAGT

Protein sequence

EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQNS

Homology

[Close]

BLAST of MS001812 vs. NCBI nr
Match: XP_022135207.1 (mitochondrial import inner membrane translocase subunit TIM8 [Momordica charantia])

HSP 1 Score: 124.4 bits (311), Expect = 3.4e-25
Identity = 61/61 (100.00%), Postives = 61/61 (100.00%), Query Frame = 0


Query: 1  EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 60
          EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

Query: 61 S 62
          S
Sbjct: 77 S 77

[Close]

BLAST of MS001812 vs. NCBI nr
Match: XP_008464391.1 (PREDICTED: mitochondrial import inner membrane translocase subunit TIM8 [Cucumis melo] >XP_011649996.1 mitochondrial import inner membrane translocase subunit TIM8 [Cucumis sativus] >XP_022921432.1 mitochondrial import inner membrane translocase subunit TIM8 [Cucurbita moschata] >XP_022924732.1 mitochondrial import inner membrane translocase subunit TIM8-like [Cucurbita moschata] >XP_022974369.1 mitochondrial import inner membrane translocase subunit TIM8-like [Cucurbita maxima] >XP_022988609.1 mitochondrial import inner membrane translocase subunit TIM8 [Cucurbita maxima] >XP_023516926.1 mitochondrial import inner membrane translocase subunit TIM8 [Cucurbita pepo subsp. pepo] >XP_023529319.1 mitochondrial import inner membrane translocase subunit TIM8-like [Cucurbita pepo subsp. pepo] >XP_038878330.1 mitochondrial import inner membrane translocase subunit TIM8 [Benincasa hispida] >KGN63456.1 hypothetical protein Csa_014027 [Cucumis sativus])

HSP 1 Score: 123.2 bits (308), Expect = 7.5e-25
Identity = 60/61 (98.36%), Postives = 61/61 (100.00%), Query Frame = 0


Query: 1  EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 60
          EEKQRAMVAEMVAKLTSVCWDKCITG+PGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWDKCITGTPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

Query: 61 S 62
          S
Sbjct: 77 S 77

[Close]

BLAST of MS001812 vs. NCBI nr
Match: KAG7023044.1 (Mitochondrial import inner membrane translocase subunit TIM8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 118.6 bits (296), Expect = 1.8e-23
Identity = 57/60 (95.00%), Postives = 59/60 (98.33%), Query Frame = 0


Query: 1  EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 60
          EEKQRAMVAEMVAKLTSVCW KC+TG+PGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWTKCVTGTPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

[Close]

BLAST of MS001812 vs. NCBI nr
Match: XP_028764582.1 (mitochondrial import inner membrane translocase subunit TIM8-like [Prosopis alba] >XP_028765220.1 mitochondrial import inner membrane translocase subunit TIM8-like [Prosopis alba])

HSP 1 Score: 111.3 bits (277), Expect = 2.9e-21
Identity = 52/60 (86.67%), Postives = 57/60 (95.00%), Query Frame = 0


Query: 1  EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 60
          +EK+RAMV EMVAKLTS CWDKCITG+PGSKFSSSES CLSNCAQRYMD+SI+IMKRFQN
Sbjct: 17 QEKERAMVNEMVAKLTSACWDKCITGTPGSKFSSSESTCLSNCAQRYMDLSILIMKRFQN 76

[Close]

BLAST of MS001812 vs. NCBI nr
Match: XP_010250594.1 (PREDICTED: mitochondrial import inner membrane translocase subunit TIM8 [Nelumbo nucifera])

HSP 1 Score: 110.9 bits (276), Expect = 3.9e-21
Identity = 52/60 (86.67%), Postives = 58/60 (96.67%), Query Frame = 0


Query: 1  EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 60
          +EKQ+AMV EMVAKLT+VCWDKCITG+PGSKFSSSES CLSNCAQRYMDMS+IIMKRFQ+
Sbjct: 17 QEKQKAMVNEMVAKLTNVCWDKCITGTPGSKFSSSESTCLSNCAQRYMDMSLIIMKRFQS 76

[Close]

BLAST of MS001812 vs. ExPASy Swiss-Prot
Match: Q9XGY4 (Mitochondrial import inner membrane translocase subunit TIM8 OS=Arabidopsis thaliana OX=3702 GN=TIM8 PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 1.4e-21
Identity = 47/60 (78.33%), Postives = 57/60 (95.00%), Query Frame = 0


Query: 1  EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 60
          +EK+RAMV EMV+K+TSVCWDKCIT +PGSKFSSSES+CL++CAQRYMDMS+IIMKRF +
Sbjct: 17 QEKERAMVNEMVSKMTSVCWDKCITSAPGSKFSSSESSCLTHCAQRYMDMSMIIMKRFNS 76

[Close]

BLAST of MS001812 vs. ExPASy Swiss-Prot
Match: P0CR95 (Mitochondrial import inner membrane translocase subunit TIM8 OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=TIM8 PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.0e-08
Identity = 24/59 (40.68%), Postives = 40/59 (67.80%), Query Frame = 0


Query: 1  EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQ 60
          +E+ +A +   + +LT+ CW+ CITG   SKFS SE+ CL NC  R++D S+ I+++ +
Sbjct: 24 QEQAKAKLQASIHELTNTCWNTCITGGISSKFSKSEAQCLENCVDRFLDSSLYIVRQIE 82

[Close]

BLAST of MS001812 vs. ExPASy Swiss-Prot
Match: P0CR94 (Mitochondrial import inner membrane translocase subunit TIM8 OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) OX=214684 GN=TIM8 PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.0e-08
Identity = 24/59 (40.68%), Postives = 40/59 (67.80%), Query Frame = 0


Query: 1  EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQ 60
          +E+ +A +   + +LT+ CW+ CITG   SKFS SE+ CL NC  R++D S+ I+++ +
Sbjct: 24 QEQAKAKLQASIHELTNTCWNTCITGGISSKFSKSEAQCLENCVDRFLDSSLYIVRQIE 82

[Close]

BLAST of MS001812 vs. ExPASy Swiss-Prot
Match: Q9Y1A3 (Mitochondrial import inner membrane translocase subunit Tim8 OS=Drosophila melanogaster OX=7227 GN=Tim8 PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 8.7e-08
Identity = 24/57 (42.11%), Postives = 36/57 (63.16%), Query Frame = 0


Query: 2  EKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRF 59
          EKQ+A V   + +   +CW+KCI G P +K   +   CLSNC  R++D S++I +RF
Sbjct: 21 EKQKAQVNAQIHEFNEICWEKCI-GKPSTKLDHATETCLSNCVDRFIDTSLLITQRF 76

[Close]

BLAST of MS001812 vs. ExPASy Swiss-Prot
Match: Q09783 (Mitochondrial import inner membrane translocase subunit tim8 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tim8 PE=3 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 5.6e-07
Identity = 23/57 (40.35%), Postives = 37/57 (64.91%), Query Frame = 0


Query: 2  EKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRF 59
          E+Q+  + + + + TS CW KCI G+ G+K   SE  CL NC +R++D +  I+KR+
Sbjct: 27 EQQKVKLQQAIHQFTSTCWPKCI-GNIGNKLDKSEEQCLQNCVERFLDCNFHIIKRY 82

[Close]

BLAST of MS001812 vs. ExPASy TrEMBL
Match: A0A6J1C212 (Mitochondrial import inner membrane translocase subunit OS=Momordica charantia OX=3673 GN=LOC111007226 PE=3 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.6e-25
Identity = 61/61 (100.00%), Postives = 61/61 (100.00%), Query Frame = 0


Query: 1  EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 60
          EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

Query: 61 S 62
          S
Sbjct: 77 S 77

[Close]

BLAST of MS001812 vs. ExPASy TrEMBL
Match: A0A6J1IHF0 (Mitochondrial import inner membrane translocase subunit OS=Cucurbita maxima OX=3661 GN=LOC111472993 PE=3 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 3.6e-25
Identity = 60/61 (98.36%), Postives = 61/61 (100.00%), Query Frame = 0


Query: 1  EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 60
          EEKQRAMVAEMVAKLTSVCWDKCITG+PGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWDKCITGTPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

Query: 61 S 62
          S
Sbjct: 77 S 77

[Close]

BLAST of MS001812 vs. ExPASy TrEMBL
Match: A0A6J1E0G1 (Mitochondrial import inner membrane translocase subunit OS=Cucurbita moschata OX=3662 GN=LOC111429714 PE=3 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 3.6e-25
Identity = 60/61 (98.36%), Postives = 61/61 (100.00%), Query Frame = 0


Query: 1  EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 60
          EEKQRAMVAEMVAKLTSVCWDKCITG+PGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWDKCITGTPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

Query: 61 S 62
          S
Sbjct: 77 S 77

[Close]

BLAST of MS001812 vs. ExPASy TrEMBL
Match: A0A0A0LTY7 (Mitochondrial import inner membrane translocase subunit OS=Cucumis sativus OX=3659 GN=Csa_1G001280 PE=3 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 3.6e-25
Identity = 60/61 (98.36%), Postives = 61/61 (100.00%), Query Frame = 0


Query: 1  EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 60
          EEKQRAMVAEMVAKLTSVCWDKCITG+PGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWDKCITGTPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

Query: 61 S 62
          S
Sbjct: 77 S 77

[Close]

BLAST of MS001812 vs. ExPASy TrEMBL
Match: A0A1S3CLD0 (Mitochondrial import inner membrane translocase subunit OS=Cucumis melo OX=3656 GN=LOC103502294 PE=3 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 3.6e-25
Identity = 60/61 (98.36%), Postives = 61/61 (100.00%), Query Frame = 0


Query: 1  EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 60
          EEKQRAMVAEMVAKLTSVCWDKCITG+PGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN
Sbjct: 17 EEKQRAMVAEMVAKLTSVCWDKCITGTPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 76

Query: 61 S 62
          S
Sbjct: 77 S 77

[Close]

BLAST of MS001812 vs. TAIR 10
Match: AT5G50810.1 (translocase inner membrane subunit 8 )

HSP 1 Score: 102.8 bits (255), Expect = 9.8e-23
Identity = 47/60 (78.33%), Postives = 57/60 (95.00%), Query Frame = 0


Query: 1  EEKQRAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMKRFQN 60
          +EK+RAMV EMV+K+TSVCWDKCIT +PGSKFSSSES+CL++CAQRYMDMS+IIMKRF +
Sbjct: 17 QEKERAMVNEMVSKMTSVCWDKCITSAPGSKFSSSESSCLTHCAQRYMDMSMIIMKRFNS 76

[Close]

BLAST of MS001812 vs. TAIR 10
Match: AT1G61570.1 (translocase of the inner mitochondrial membrane 13 )

HSP 1 Score: 50.1 bits (118), Expect = 7.5e-07
Identity = 22/52 (42.31%), Postives = 34/52 (65.38%), Query Frame = 0


Query: 5  RAMVAEMVAKLTSVCWDKCITGSPGSKFSSSESNCLSNCAQRYMDMSIIIMK 57
          +A   E++  L + C+DKC+T  PGS    SES+C+S C +RYM+ + II +
Sbjct: 31 QAYAEELIETLRTKCFDKCVT-KPGSSLGGSESSCISRCVERYMEATAIISR 81

The following BLAST results are available for this feature:

BLAST of MS001812 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135207.1	3.4e-25	100.00	mitochondrial import inner membrane translocase subunit TIM8 [Momordica charanti...	[more]
XP_008464391.1	7.5e-25	98.36	PREDICTED: mitochondrial import inner membrane translocase subunit TIM8 [Cucumis...	[more]
KAG7023044.1	1.8e-23	95.00	Mitochondrial import inner membrane translocase subunit TIM8, partial [Cucurbita...	[more]
XP_028764582.1	2.9e-21	86.67	mitochondrial import inner membrane translocase subunit TIM8-like [Prosopis alba...	[more]
XP_010250594.1	3.9e-21	86.67	PREDICTED: mitochondrial import inner membrane translocase subunit TIM8 [Nelumbo...	[more]

BLAST of MS001812 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XGY4	1.4e-21	78.33	Mitochondrial import inner membrane translocase subunit TIM8 OS=Arabidopsis thal...	[more]
P0CR95	1.0e-08	40.68	Mitochondrial import inner membrane translocase subunit TIM8 OS=Cryptococcus neo...	[more]
P0CR94	1.0e-08	40.68	Mitochondrial import inner membrane translocase subunit TIM8 OS=Cryptococcus neo...	[more]
Q9Y1A3	8.7e-08	42.11	Mitochondrial import inner membrane translocase subunit Tim8 OS=Drosophila melan...	[more]
Q09783	5.6e-07	40.35	Mitochondrial import inner membrane translocase subunit tim8 OS=Schizosaccharomy...	[more]

BLAST of MS001812 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C212	1.6e-25	100.00	Mitochondrial import inner membrane translocase subunit OS=Momordica charantia O...	[more]
A0A6J1IHF0	3.6e-25	98.36	Mitochondrial import inner membrane translocase subunit OS=Cucurbita maxima OX=3...	[more]
A0A6J1E0G1	3.6e-25	98.36	Mitochondrial import inner membrane translocase subunit OS=Cucurbita moschata OX...	[more]
A0A0A0LTY7	3.6e-25	98.36	Mitochondrial import inner membrane translocase subunit OS=Cucumis sativus OX=36...	[more]
A0A1S3CLD0	3.6e-25	98.36	Mitochondrial import inner membrane translocase subunit OS=Cucumis melo OX=3656 ...	[more]

BLAST of MS001812 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G50810.1	9.8e-23	78.33	translocase inner membrane subunit 8	[more]
AT1G61570.1	7.5e-07	42.31	translocase of the inner mitochondrial membrane 13	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004217	Tim10-like	PFAM	PF02953	zf-Tim10_DDP	coord: 1..60 e-value: 4.7E-24 score: 83.8
IPR035427	Tim10-like domain superfamily	GENE3D	1.10.287.810	Mitochondrial import inner membrane translocase subunit tim13 like domains	coord: 1..61 e-value: 6.7E-23 score: 81.8
IPR035427	Tim10-like domain superfamily	SUPERFAMILY	144122	Tim10-like	coord: 4..60
IPR039238	Mitochondrial import inner membrane translocase subunit Tim8/13	PANTHER	PTHR19338	TRANSLOCASE OF INNER MITOCHONDRIAL MEMBRANE 13 HOMOLOG	coord: 2..60
None	No IPR available	PANTHER	PTHR19338:SF15	MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM8-LIKE	coord: 2..60

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS001812.1	MS001812.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005758	mitochondrial intermembrane space