MS001735 (gene) Bitter gourd (TR) v1

Overview
NameMS001735
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
Locationscaffold571: 571546 .. 575245 (+)
RNA-Seq ExpressionMS001735
SyntenyMS001735
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCAAAATTACTCAGGCTACCATCACTCTCAAACACACCCTATAAACGGTTTCATTTTTCAGGTTTATATTTCTGATATGACAGCTTTATTTACGTGGACATGCTCAAGATGCAATGGCTTCACTGAGGTACTTGGAGAAATGAAATGGCTTATATCCAATAAACTCCAGCTCCTAAAATGAGATTTTCTGTTATCCAGCCTCTTCACGTGTTCAAGTTTTTGGTATTAATGCTTATTTTCCTTTGATACAGGGGTTTGAAGTTGTGGAGTTGGTTTTTGATGTTGAAAGTTGCCTTCAGGTGCATAATTATAGGATTTTTTCCTGAGTTAACTACCTTGCATTTACCTATTGCTTCTTGTATCTATGCTGATATCACCATCTGCTGCAGTCATTTGTTGGAGTAGCCAAGGATCCACGTGCTATTATAATTGCATTCAGAGGAACACGGGGAAGTAGGTACAATGCTTTTAAGAGTTTTATATTTAAGTAACTTCAGTTAAATATCCTCCCTGAGCTTTGAGTTTTTAGTGGTTTGTTGAAATAATGAACAAGAAGTTTTTGCTGTTTTTGTTGCGTGGCAAGGGGAGCCCATTGTCGAGGCTAGATCTCTTTTGGTCATATCGGCTTAGAGGTCTCAAGTTCGAACCTCCGGGTGAGCTTAATAAAAAAATCCGTCATATCTCTCACGTCCGAGCCTTAAGGTGGGCGCGGGTGCCCCGGATATAGGAGAACAAAGATCTGACACCTGATTATAAAAAAAGAAAAAGAAGAAGAAGAAGTTTTTGCTGTTTCCTTTGTGTGGCTACTGAAAGCAACATGAGGAGAAAGATAATGATAGAAAAACTTGGATTTTCCAAAATTTTGGGCAGTATTCCACATTCGTGATAATATAATATTAAAATATACATGTATGGCATTTCCTCTCAAGCAAATTGCTTTATAGACGAGTGGAATAATTCAGATTTACAGGTTACGAAGCCCACATGAAAATGGTATCCAATATTCCAATTCTAAGAATTTCTAGCTCAGCGGTGTTGTCAATTGTCGTTTTCTTTTTACTGATTTTAGTACTCGATTAGCCTTCTGTTATCAACTGTTATCTTTGATAATGCTCAGTCATTAGGCGTTGGTTGCTTGATTTTTTTTTCCTTTGGAAAACATCACTCATTCTTTGCAAAGATAAATATATTCTTGTACGTAACCCATCAAGATTTGACGTGAAGGTTTTGGTTAATCTTTGTCTTCTTGATTTTAAATCCTGAGATTGACCTTCTCTGCAGCATACAGAACTGGATAGAAGACTTATTTTGGAAACAGCTAGATTTAGATTATCCTGGAATGCCGGGTGCAAAGGTGTGTTGTTACTAGAACTTGAAGTTCTCTTGGTCTGTACTTATTCAGCTGAATTTTAAGAGCATCACTTTCTTCGTTTTTGTTCCACCATCATGTTAGGTACATAGTGGATTTTATCGTGCTTATCACTGTACAACAATGCGCCCTGCAATTTTAAATGCAGTTAAAAGAGCAAAGGAAGTTTATGGGGACCTTGACATCATTGTGACAGGCCATTCAATGGGGGGAGCAATGGCCGCTTTTTGTGCTTTTGATCTTATAGTAAGTGATATTTGTTCCTTAACGGCTTGCAAAAATCTTTTTGGTTTTGCAGAACTAGGCTCATTTGCATGTTTTAGATCGAATTTGTGTGTATGGAAGAACAAATGGAGTTTTGGATTCCATTTTCATGGATTTTGCCTATTTCTTCTTTTATTCCAAGTCCATTTTCTCTCTGGTGATCCTTGTGAATTGGAGCTCATCTTAATTTAGCTACAACCCTGAAAATCACCCGTTATGATCTCTTGTGACTCACTCACTCTCAGGGGTGCAAGATGAAAATAAAAAAACTGTGTAGATTTAGTTTATTTGAAGTATACTTATCTGTTGGTTCGTATAAAGTCAGCAGTAGTTGATTTGGATATATTTTCTTGATTTTTGTTTGTTCGAGCAGGATAGTAATTTTTTTCACATAAATAAAATAAACCAGTTTGGTATCAATTATTTATTTTCCTTAAACCGGTTTACCGGTTTGAAGTCGAGTTTGGCTGAAAACCGATCTAAACCTACTCAGTTGTACCTACAAGTTTTAATGGCATCTTTGTTCAGTATCTAAGGTTTATGGATTTGATTATTTATCTTATAGTTGAGCATTTCCTCACAGGTTAATAATAAAGCCTCAAACGTTCAAGTTATCACATTCGGCCAACCTCGCATTGGCAATGCAGCTTTTGCATCTCACTACAGCAAACATTTGCCGAATACTATTCGAGTCACAAATGGACATGATATGGTGCCCCATTTGCCTCCCTACTTTTTCATTTTCCCTCGAAGAACATATCACCACTTCCCAAGAGAGGTACAAATTTGCTTTATGGTGAATTATGCATAAGTTTGCTGGAAACAGAAGAATTATTTAGATTTACTTAGATCGGTTGGTAGATCTTTTATTTGTTAGCCAAAGCTCAGAATTCATCGCCATGGATTTTATGGGCAGAGTTGGATGTTTTTTTTTTCTCTTCCTAGATTAGCGGACGTATTTTCTCTTTTTCAGGTATGGCTTCAGGGTACTTCATCGAAATGTTTTGCCTCCTACACTGAGATTGTCTGTGATGATTCTGGCGAAGATCTAACTTGTAGCAGGTAAATCTCAGCGTTTGTTGTTAATATAGTAGTAAAAGTTGCACACTTAACTAGCTATTCTTTCAGCGTTTGGTTTTATTTCCGGGTGCAAGGTGGTCCCTTCACTTAGACAAACTCAGTTGAATTAATGATCTGAAAATCCTAGTGAATTGGAACTAACAACCATTTGAGTATGTGAATTCCTAATTCACAAACACTAGTTCGATTTCAACTCTAAGGTTTAGGTTAGACATGGGTTACTTTAAGATCAAACCTCTTGAGACAAAGAACAACTTCCTTAGGAGTCGAACACTTTGAAGATAGAGTGATCAATCCCACTTTGATGTTCTTGACGTGCAACTCAAAGAAACATAAATTGCAAAAGTTTAAGAAGCTATTGGCTAACTTGAAAGGAAAAGCTCAATGACTAAAATCTATTCAACCTCATGGCAAGATTTCAGCTAGCCTCCCAACTAAACTCTGGATATCACATCGTAAGGGTACAATTGGAAAGTAATAAAATTACATAAAAGAACTCTTAAATAAAAATATATTATAATTTGAAGTTCTAGGCAGTTCATATCCATTGGTTTTGTTAAATTTTTTGGATTAAAATACCTTCTCTGTGTAAAGTTCTATATCCATCCTCCCTGATTTGTCTTGATACTGATGAAATCTGCTGCAAAAGGTTTCATGTTGATGAGTAGATGAACTTCAGCAACATATTGATCACCAAGTACCGTATCCAAGTTAGAAGAAGCATGAGATAGCATTACTTTATAAGAATCAGCCATCTTTTGAAAAAACTTGCAATTTCATTAAATGCAGATCTGTAATTGGGAACAGCATTCAGGACCATTTGTCCTATTATGGTGTGGAATTCCCAACTGATGATCCAGGAACATGTTGGATCGTTATGGATCCGTTGCTGGTCGAATACGGATCGATCGATTCGAAAGGTAATATCATGTTGTTCAGAGATCCTGCTACTCCAATTCCCATAATACCGACTTTAGAGGCAAAT

mRNA sequence

TTTCAAAATTACTCAGGCTACCATCACTCTCAAACACACCCTATAAACGGTTTCATTTTTCAGGTTTATATTTCTGATATGACAGCTTTATTTACGTGGACATGCTCAAGATGCAATGGCTTCACTGAGGGGTTTGAAGTTGTGGAGTTGGTTTTTGATGTTGAAAGTTGCCTTCAGTCATTTGTTGGAGTAGCCAAGGATCCACGTGCTATTATAATTGCATTCAGAGGAACACGGGGAAGTAGCATACAGAACTGGATAGAAGACTTATTTTGGAAACAGCTAGATTTAGATTATCCTGGAATGCCGGGTGCAAAGGTACATAGTGGATTTTATCGTGCTTATCACTGTACAACAATGCGCCCTGCAATTTTAAATGCAGTTAAAAGAGCAAAGGAAGTTTATGGGGACCTTGACATCATTGTGACAGGCCATTCAATGGGGGGAGCAATGGCCGCTTTTTGTGCTTTTGATCTTATAGTTAATAATAAAGCCTCAAACGTTCAAGTTATCACATTCGGCCAACCTCGCATTGGCAATGCAGCTTTTGCATCTCACTACAGCAAACATTTGCCGAATACTATTCGAGTCACAAATGGACATGATATGGTGCCCCATTTGCCTCCCTACTTTTTCATTTTCCCTCGAAGAACATATCACCACTTCCCAAGAGAGGTATGGCTTCAGGGTACTTCATCGAAATGTTTTGCCTCCTACACTGAGATTGTCTGTGATGATTCTGGCGAAGATCTAACTTGTAGCAGATCTGTAATTGGGAACAGCATTCAGGACCATTTGTCCTATTATGGTGTGGAATTCCCAACTGATGATCCAGGAACATGTTGGATCGTTATGGATCCGTTGCTGGTCGAATACGGATCGATCGATTCGAAAGGTAATATCATGTTGTTCAGAGATCCTGCTACTCCAATTCCCATAATACCGACTTTAGAGGCAAAT

Coding sequence (CDS)

TTTCAAAATTACTCAGGCTACCATCACTCTCAAACACACCCTATAAACGGTTTCATTTTTCAGGTTTATATTTCTGATATGACAGCTTTATTTACGTGGACATGCTCAAGATGCAATGGCTTCACTGAGGGGTTTGAAGTTGTGGAGTTGGTTTTTGATGTTGAAAGTTGCCTTCAGTCATTTGTTGGAGTAGCCAAGGATCCACGTGCTATTATAATTGCATTCAGAGGAACACGGGGAAGTAGCATACAGAACTGGATAGAAGACTTATTTTGGAAACAGCTAGATTTAGATTATCCTGGAATGCCGGGTGCAAAGGTACATAGTGGATTTTATCGTGCTTATCACTGTACAACAATGCGCCCTGCAATTTTAAATGCAGTTAAAAGAGCAAAGGAAGTTTATGGGGACCTTGACATCATTGTGACAGGCCATTCAATGGGGGGAGCAATGGCCGCTTTTTGTGCTTTTGATCTTATAGTTAATAATAAAGCCTCAAACGTTCAAGTTATCACATTCGGCCAACCTCGCATTGGCAATGCAGCTTTTGCATCTCACTACAGCAAACATTTGCCGAATACTATTCGAGTCACAAATGGACATGATATGGTGCCCCATTTGCCTCCCTACTTTTTCATTTTCCCTCGAAGAACATATCACCACTTCCCAAGAGAGGTATGGCTTCAGGGTACTTCATCGAAATGTTTTGCCTCCTACACTGAGATTGTCTGTGATGATTCTGGCGAAGATCTAACTTGTAGCAGATCTGTAATTGGGAACAGCATTCAGGACCATTTGTCCTATTATGGTGTGGAATTCCCAACTGATGATCCAGGAACATGTTGGATCGTTATGGATCCGTTGCTGGTCGAATACGGATCGATCGATTCGAAAGGTAATATCATGTTGTTCAGAGATCCTGCTACTCCAATTCCCATAATACCGACTTTAGAGGCAAAT

Protein sequence

FQNYSGYHHSQTHPINGFIFQVYISDMTALFTWTCSRCNGFTEGFEVVELVFDVESCLQSFVGVAKDPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTMRPAILNAVKRAKEVYGDLDIIVTGHSMGGAMAAFCAFDLIVNNKASNVQVITFGQPRIGNAAFASHYSKHLPNTIRVTNGHDMVPHLPPYFFIFPRRTYHHFPREVWLQGTSSKCFASYTEIVCDDSGEDLTCSRSVIGNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVEYGSIDSKGNIMLFRDPATPIPIIPTLEAN
Homology
BLAST of MS001735 vs. NCBI nr
Match: XP_022138651.1 (lipase-like isoform X1 [Momordica charantia] >XP_022138654.1 lipase-like isoform X1 [Momordica charantia])

HSP 1 Score: 637.9 bits (1644), Expect = 4.8e-179
Identity = 302/316 (95.57%), Postives = 306/316 (96.84%), Query Frame = 0

Query: 5   SGYHHSQTHPINGFIFQVYISDMTALFTWTCSRCNGFTEGFEVVELVFDVESCLQSFVGV 64
           S Y+H+    +  +   VYISDMTALFTWTCSRCNGFTEGFEVVELVFDVESCLQSFVGV
Sbjct: 35  SVYNHTLAIIMVEYASAVYISDMTALFTWTCSRCNGFTEGFEVVELVFDVESCLQSFVGV 94

Query: 65  AKDPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTMRPAI 124
           AKDPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTMRPAI
Sbjct: 95  AKDPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTMRPAI 154

Query: 125 LNAVKRAKEVYGDLDIIVTGHSMGGAMAAFCAFDLIVNNKASNVQVITFGQPRIGNAAFA 184
           LNAVKRAKEVYGDLDIIVTGHSMGGAMAAFCAFDLIVNNKASNVQVITFGQPRIGNAAFA
Sbjct: 155 LNAVKRAKEVYGDLDIIVTGHSMGGAMAAFCAFDLIVNNKASNVQVITFGQPRIGNAAFA 214

Query: 185 SHYSKHLPNTIRVTNGHDMVPHLPPYFFIFPRRTYHHFPREVWLQGTSSKCFASYTEIVC 244
           SHYSKHLPNTIRVTNGHDMVPHLPPYFFIFPRRTYHHFPREVWLQGTSSKCFASYTEIVC
Sbjct: 215 SHYSKHLPNTIRVTNGHDMVPHLPPYFFIFPRRTYHHFPREVWLQGTSSKCFASYTEIVC 274

Query: 245 DDSGEDLTCSRSVIGNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVEYGSIDSKGNIMLF 304
           DDSGEDLTCSRSVIGNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVEYGSIDSKGNIMLF
Sbjct: 275 DDSGEDLTCSRSVIGNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVEYGSIDSKGNIMLF 334

Query: 305 RDPATPIPIIPTLEAN 321
           RDPATPIPIIPTLEAN
Sbjct: 335 RDPATPIPIIPTLEAN 350

BLAST of MS001735 vs. NCBI nr
Match: XP_038875858.1 (lipase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 543.1 bits (1398), Expect = 1.6e-150
Identity = 255/315 (80.95%), Postives = 281/315 (89.21%), Query Frame = 0

Query: 7   YHHSQTHPINGFIFQVYISDMTALFTWTCSRCNGFTEGFEVVELVFDVESCLQSFVGVAK 66
           Y+H+    +  +   VYISDMTALFTWTCSRC+G TEGFEVV+LV DVESCLQS+VGVAK
Sbjct: 41  YNHTFAIVMVEYASAVYISDMTALFTWTCSRCHGLTEGFEVVQLVVDVESCLQSYVGVAK 100

Query: 67  DPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTMRPAILN 126
           +PRAIIIAFRGTRG+SIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTT+RPAILN
Sbjct: 101 NPRAIIIAFRGTRGTSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTIRPAILN 160

Query: 127 AVKRAKEVYGDLDIIVTGHSMGGAMAAFCAFDLIVNNKASNVQVITFGQPRIGNAAFASH 186
           AVK AKEVYGDLDIIVTGHSMGGA+AAFCA DLIVN+ +SNVQV+TFGQPRIGNAAFAS+
Sbjct: 161 AVKEAKEVYGDLDIIVTGHSMGGAIAAFCALDLIVNHNSSNVQVVTFGQPRIGNAAFASY 220

Query: 187 YSKHLPNTIRVTNGHDMVPHLPPYFFIFPRRTYHHFPREVWLQGTS--SKCFASYTEIVC 246
           YSKHLP TIRVT+GHD+VPHLPPYF +  R+TYHHFPREVWLQ TS  S C A  +E VC
Sbjct: 221 YSKHLPQTIRVTHGHDIVPHLPPYFSMLRRKTYHHFPREVWLQDTSSTSTCLALDSETVC 280

Query: 247 DDSGEDLTCSRSVIGNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVEYGSIDSKGNIMLF 306
           DDSGED  CSRSV+GNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVEYGS+DS+GN+MLF
Sbjct: 281 DDSGEDPNCSRSVVGNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVEYGSVDSEGNVMLF 340

Query: 307 RDPATPIPIIPTLEA 320
           ++PA PI    T EA
Sbjct: 341 KNPAIPISRAQTAEA 355

BLAST of MS001735 vs. NCBI nr
Match: XP_038875859.1 (lipase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 543.1 bits (1398), Expect = 1.6e-150
Identity = 255/315 (80.95%), Postives = 281/315 (89.21%), Query Frame = 0

Query: 7   YHHSQTHPINGFIFQVYISDMTALFTWTCSRCNGFTEGFEVVELVFDVESCLQSFVGVAK 66
           Y+H+    +  +   VYISDMTALFTWTCSRC+G TEGFEVV+LV DVESCLQS+VGVAK
Sbjct: 11  YNHTFAIVMVEYASAVYISDMTALFTWTCSRCHGLTEGFEVVQLVVDVESCLQSYVGVAK 70

Query: 67  DPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTMRPAILN 126
           +PRAIIIAFRGTRG+SIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTT+RPAILN
Sbjct: 71  NPRAIIIAFRGTRGTSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTIRPAILN 130

Query: 127 AVKRAKEVYGDLDIIVTGHSMGGAMAAFCAFDLIVNNKASNVQVITFGQPRIGNAAFASH 186
           AVK AKEVYGDLDIIVTGHSMGGA+AAFCA DLIVN+ +SNVQV+TFGQPRIGNAAFAS+
Sbjct: 131 AVKEAKEVYGDLDIIVTGHSMGGAIAAFCALDLIVNHNSSNVQVVTFGQPRIGNAAFASY 190

Query: 187 YSKHLPNTIRVTNGHDMVPHLPPYFFIFPRRTYHHFPREVWLQGTS--SKCFASYTEIVC 246
           YSKHLP TIRVT+GHD+VPHLPPYF +  R+TYHHFPREVWLQ TS  S C A  +E VC
Sbjct: 191 YSKHLPQTIRVTHGHDIVPHLPPYFSMLRRKTYHHFPREVWLQDTSSTSTCLALDSETVC 250

Query: 247 DDSGEDLTCSRSVIGNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVEYGSIDSKGNIMLF 306
           DDSGED  CSRSV+GNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVEYGS+DS+GN+MLF
Sbjct: 251 DDSGEDPNCSRSVVGNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVEYGSVDSEGNVMLF 310

Query: 307 RDPATPIPIIPTLEA 320
           ++PA PI    T EA
Sbjct: 311 KNPAIPISRAQTAEA 325

BLAST of MS001735 vs. NCBI nr
Match: XP_022930304.1 (lipase-like isoform X1 [Cucurbita moschata] >XP_022930305.1 lipase-like isoform X1 [Cucurbita moschata] >XP_022930306.1 lipase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 536.6 bits (1381), Expect = 1.5e-148
Identity = 252/317 (79.50%), Postives = 278/317 (87.70%), Query Frame = 0

Query: 5   SGYHHSQTHPINGFIFQVYISDMTALFTWTCSRCNGFTEGFEVVELVFDVESCLQSFVGV 64
           S Y+H+    +  +   VYISDMT+LFTWTC+RC G TEGFEVV+L+ DVESCLQSFVGV
Sbjct: 39  SVYNHTLAMIMVEYASAVYISDMTSLFTWTCTRCRGLTEGFEVVQLIVDVESCLQSFVGV 98

Query: 65  AKDPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTMRPAI 124
           AKDPRAIIIAFRGTRG+SIQNWIEDLFWKQLDLDYPGM GAKVHSGFYRAYHCTT+RPAI
Sbjct: 99  AKDPRAIIIAFRGTRGTSIQNWIEDLFWKQLDLDYPGMEGAKVHSGFYRAYHCTTIRPAI 158

Query: 125 LNAVKRAKEVYGDLDIIVTGHSMGGAMAAFCAFDLIVNNKASNVQVITFGQPRIGNAAFA 184
           LNAVK AKEVYGDLD IVTGHSMGGA+AAFCA DLIVN+   NVQV+TFGQPRIGNA FA
Sbjct: 159 LNAVKTAKEVYGDLDTIVTGHSMGGAIAAFCALDLIVNHNVPNVQVMTFGQPRIGNAVFA 218

Query: 185 SHYSKHLPNTIRVTNGHDMVPHLPPYFFIFPRRTYHHFPREVWLQGTSSKC--FASYTEI 244
           S+YSKHLP+TIRVT+ HD+VPHLPPYF I PR+TYHHFPREVWLQ TSS     ASY E 
Sbjct: 219 SYYSKHLPDTIRVTHEHDIVPHLPPYFSIIPRKTYHHFPREVWLQDTSSTSNRLASYIET 278

Query: 245 VCDDSGEDLTCSRSVIGNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVEYGSIDSKGNIM 304
           VCDDSGED  CSRSVIGNSIQDHLSYYGVEFPTDDP TCWIVMDP++ EYGSIDS+GN++
Sbjct: 279 VCDDSGEDPNCSRSVIGNSIQDHLSYYGVEFPTDDPATCWIVMDPVIAEYGSIDSEGNVV 338

Query: 305 LFRDPATPIPIIPTLEA 320
           LFRD ATP+P + +LEA
Sbjct: 339 LFRDLATPVPRMQSLEA 355

BLAST of MS001735 vs. NCBI nr
Match: KAG6593948.1 (hypothetical protein SDJN03_13424, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 535.8 bits (1379), Expect = 2.5e-148
Identity = 252/317 (79.50%), Postives = 277/317 (87.38%), Query Frame = 0

Query: 5   SGYHHSQTHPINGFIFQVYISDMTALFTWTCSRCNGFTEGFEVVELVFDVESCLQSFVGV 64
           S Y+H+    +  +   VYISDMT+LFTWTC+RC G TEGFEVV+L+ DVESCLQSFVGV
Sbjct: 39  SVYNHTLAMIMVEYASAVYISDMTSLFTWTCTRCRGLTEGFEVVQLIVDVESCLQSFVGV 98

Query: 65  AKDPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTMRPAI 124
           AKDPRAIIIAFRGTRG+SIQNWIEDLFWKQLDLDYPGM GAKVHSGFYRAYHCTT+RPAI
Sbjct: 99  AKDPRAIIIAFRGTRGTSIQNWIEDLFWKQLDLDYPGMEGAKVHSGFYRAYHCTTIRPAI 158

Query: 125 LNAVKRAKEVYGDLDIIVTGHSMGGAMAAFCAFDLIVNNKASNVQVITFGQPRIGNAAFA 184
           LNAVK AKEVYGDLD IVTGHSMGGA+AAFCA DLIVN+   NVQV+TFGQPRIGNA FA
Sbjct: 159 LNAVKTAKEVYGDLDTIVTGHSMGGAIAAFCALDLIVNHNVPNVQVMTFGQPRIGNAVFA 218

Query: 185 SHYSKHLPNTIRVTNGHDMVPHLPPYFFIFPRRTYHHFPREVWLQGTSSKC--FASYTEI 244
           S+YSKHLP+TIRVT+ HD+VPHLPPYF I PR+TYHHFPREVWLQ TSS     ASY E 
Sbjct: 219 SYYSKHLPDTIRVTHEHDIVPHLPPYFSIIPRKTYHHFPREVWLQDTSSTSNRLASYIET 278

Query: 245 VCDDSGEDLTCSRSVIGNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVEYGSIDSKGNIM 304
           VCDDSGED  CSRSVIGNSIQDHLSYYGVEFPTDDP TCWIVMDP + EYGSIDS+GN++
Sbjct: 279 VCDDSGEDPNCSRSVIGNSIQDHLSYYGVEFPTDDPATCWIVMDPAITEYGSIDSEGNVV 338

Query: 305 LFRDPATPIPIIPTLEA 320
           LFRD ATP+P + +LEA
Sbjct: 339 LFRDLATPVPRMQSLEA 355

BLAST of MS001735 vs. ExPASy Swiss-Prot
Match: P19515 (Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2)

HSP 1 Score: 129.0 bits (323), Expect = 9.4e-29
Identity = 88/249 (35.34%), Postives = 128/249 (51.41%), Query Frame = 0

Query: 32  TWTCSRCNGFTEGFEVVE----LVFDVESCLQSFVGVAKDPRAIIIAFRGTRGSSIQNWI 91
           TW C  C+  TE  ++++    L++D      + V      + I I FRG+  SSI+NWI
Sbjct: 131 TWDCIHCDA-TEDLKIIKTWSTLIYDT----NAMVARGDSEKTIYIVFRGS--SSIRNWI 190

Query: 92  EDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTMRPAILNAVKRAKEVYGDLDIIVTGHSM 151
            DL +  + + YP + G KVH GF  +Y    ++  ++  V    + Y    + VTGHS+
Sbjct: 191 ADLTF--VPVSYPPVSGTKVHKGFLDSY--GEVQNELVATVLDQFKQYPSYKVAVTGHSL 250

Query: 152 GGAMAAFCAFDLIVNNK---ASNVQVITFGQPRIGNAAFASH-YSKHLPNTIRVTNGHDM 211
           GGA A  CA DL    +   +SN+ + T GQPR+G+ AFA++  S  +P   R  N  D+
Sbjct: 251 GGATALLCALDLYQREEGLSSSNLFLYTQGQPRVGDPAFANYVVSTGIPYR-RTVNERDI 310

Query: 212 VPHLPPYFFIFPRRTYHHFPREVWLQGTSSKCFASYTEIVCDDSGEDLTCSRSVIG-NSI 271
           VPHLPP  F F      H   E W+   S +     T  VC    E   CS S++   S+
Sbjct: 311 VPHLPPAAFGF-----LHAGEEYWITDNSPE-----TVQVCTSDLETSDCSNSIVPFTSV 357

BLAST of MS001735 vs. ExPASy Swiss-Prot
Match: Q9XTR8 (Lipase ZK262.3 OS=Caenorhabditis elegans OX=6239 GN=ZK262.3 PE=1 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 8.0e-20
Identity = 80/271 (29.52%), Postives = 129/271 (47.60%), Query Frame = 0

Query: 61  FVGVAKDPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTM 120
           ++ V+   + I + FRGT+ SS Q  +E     +   D+ GM    + + ++R+ H  T 
Sbjct: 85  YIVVSDVLQQITVVFRGTKTSS-QLLLEGWTTLKPSSDFYGM---GLVNTYFRSGHEKTW 144

Query: 121 RPAILNAVKRAKEVYGDLDIIVTGHSMGGAMAAFCAFDLIVN--NKASNVQVITFGQPRI 180
           +  + +A+  ++  Y + D+ VTGHS+GGA+A  CA  ++ +   ++  ++V+TFG+PR+
Sbjct: 145 Q-YVQDALSISQ--YRNYDVYVTGHSLGGALAGLCAPRIVHDGLRQSQKIKVVTFGEPRV 204

Query: 181 GNAAFASHYSKHLPNTIRVTNGHDMVPHLP------------------PYFFIFPRRTYH 240
           GN  F+  Y + +P + RV +  D+VPHLP                  P   +     YH
Sbjct: 205 GNIEFSRAYDQLVPYSFRVVHSGDVVPHLPGCVKDLSYTPPAGSDGSMPCDPVSTNGGYH 264

Query: 241 HFPREVWLQG--TSSKCFASYTEIVCDDSGEDLTCSRSVIGN------SIQDHLSYYGVE 300
           H   E+W  G  T    F   T +  D   ED  CS S+  N       + DH +Y+GVE
Sbjct: 265 H-AIEIWYPGNMTQGDPFMVCTGLPRD---EDFGCSDSLKVNLGDTNQGVWDHRNYFGVE 324

Query: 301 FPTDDPGTCWIVMDPLLVEYGSIDSKGNIML 304
            P    G C    DP +   G     G + L
Sbjct: 325 VPDFGKGGC----DPSMTFKGPPTKTGVLSL 340

BLAST of MS001735 vs. ExPASy Swiss-Prot
Match: B8NIB8 (Probable feruloyl esterase A OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=faeA PE=3 SV=2)

HSP 1 Score: 91.7 bits (226), Expect = 1.7e-17
Identity = 66/230 (28.70%), Postives = 110/230 (47.83%), Query Frame = 0

Query: 51  VFDVESCLQSFVGVAKDPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLD-YPGMPGAKVHS 110
           +++ E+ +  +V      + II  FRGT   S  N   D  + Q   D  P   G  VH 
Sbjct: 62  IYNAETDINGWVLRDDSRQEIITVFRGT--GSDTNLQLDTNYTQAPFDTLPQCSGCAVHG 121

Query: 111 GFYRAYHCTTMRPAILNAVKRAKEVYGDLDIIVTGHSMGGAMAAFCAFDLIVNNKASNVQ 170
           G+Y  +   +++  +   V++    Y D  +++TGHS+G +MAA  A  L  +   +N+ 
Sbjct: 122 GYYVGW--ISVKDQVEGLVQQQASQYPDYSLVITGHSLGASMAAITAAQL--SATYNNIT 181

Query: 171 VITFGQPRIGNAAFASHYSKHLPNT-------IRVTNGHDMVPHLPPYFFIFPRRTYHHF 230
           V TFG+PR GN A+AS+  +    T        RVT+ +D +P+LPP       + Y H 
Sbjct: 182 VYTFGEPRTGNQAYASYVDETFQATNPDATKFYRVTHTNDGIPNLPP-----TSQGYVHH 241

Query: 231 PREVWLQGTSSKCFASYTEIVCDDSGEDLTCSRSVIGNSIQD-HLSYYGV 272
             E W    S +        +C   G+++ C  +  G  + D H++Y+G+
Sbjct: 242 GTEYW----SVEPHGPQNMYLC--LGDEVQCCEAQGGQGVNDAHVTYFGM 274

BLAST of MS001735 vs. ExPASy Swiss-Prot
Match: Q2UNW5 (Probable feruloyl esterase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=faeA PE=3 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 2.2e-17
Identity = 66/230 (28.70%), Postives = 109/230 (47.39%), Query Frame = 0

Query: 51  VFDVESCLQSFVGVAKDPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLD-YPGMPGAKVHS 110
           +++ E+ +  +V      + II  FRGT   S  N   D  + Q   D  P   G  VH 
Sbjct: 62  IYNAETDINGWVLRDDSRQEIITVFRGT--GSDTNLQLDTNYTQAPFDTLPQCSGCAVHG 121

Query: 111 GFYRAYHCTTMRPAILNAVKRAKEVYGDLDIIVTGHSMGGAMAAFCAFDLIVNNKASNVQ 170
           G+Y  +   +++  +   + +    Y D  ++VTGHS+G +MAA  A  L  +   +N+ 
Sbjct: 122 GYYVGW--VSVKDQVEGLIHQQASQYPDYSLVVTGHSLGASMAAITAAQL--SATYNNIT 181

Query: 171 VITFGQPRIGNAAFASHYSKHLPNT-------IRVTNGHDMVPHLPPYFFIFPRRTYHHF 230
           V TFG+PR GN A+AS+  +    T        RVT+ +D +P+LPP       + Y H 
Sbjct: 182 VYTFGEPRTGNQAYASYVDETFQATNPDATKFYRVTHTNDGIPNLPP-----TSQGYVHH 241

Query: 231 PREVWLQGTSSKCFASYTEIVCDDSGEDLTCSRSVIGNSIQD-HLSYYGV 272
             E W    S +        +C   G+++ C  +  G  + D H++Y+G+
Sbjct: 242 GTEYW----SVEPHGPQNMYLC--LGDEVQCCEAQGGQGVNDAHVTYFGM 274

BLAST of MS001735 vs. ExPASy Swiss-Prot
Match: O59952 (Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 2.4e-16
Identity = 54/172 (31.40%), Postives = 92/172 (53.49%), Query Frame = 0

Query: 61  FVGVAKDPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYH--CT 120
           F+ +    + I+++FRG+R  SI+NWI +L +   +++     G + H GF  ++     
Sbjct: 88  FLALDNTNKLIVLSFRGSR--SIENWIGNLNFDLKEIN-DICSGCRGHDGFTSSWRSVAD 147

Query: 121 TMRPAILNAVKRAKEVYGDLDIIVTGHSMGGAMAAFCAFDLIVNNKASNVQVITFGQPRI 180
           T+R  + +AV+     + D  ++ TGHS+GGA+A     DL       ++ V ++G PR+
Sbjct: 148 TLRQKVEDAVRE----HPDYRVVFTGHSLGGALATVAGADL--RGNGYDIDVFSYGAPRV 207

Query: 181 GNAAFASHYSKHLPNTI-RVTNGHDMVPHLPPYFFIFPRRTYHHFPREVWLQ 230
           GN AFA   +     T+ R+T+ +D+VP LPP  F      Y H   E W++
Sbjct: 208 GNRAFAEFLTVQTGGTLYRITHTNDIVPRLPPREF-----GYSHSSPEYWIK 245

BLAST of MS001735 vs. ExPASy TrEMBL
Match: A0A6J1CAB9 (lipase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009758 PE=4 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 2.3e-179
Identity = 302/316 (95.57%), Postives = 306/316 (96.84%), Query Frame = 0

Query: 5   SGYHHSQTHPINGFIFQVYISDMTALFTWTCSRCNGFTEGFEVVELVFDVESCLQSFVGV 64
           S Y+H+    +  +   VYISDMTALFTWTCSRCNGFTEGFEVVELVFDVESCLQSFVGV
Sbjct: 35  SVYNHTLAIIMVEYASAVYISDMTALFTWTCSRCNGFTEGFEVVELVFDVESCLQSFVGV 94

Query: 65  AKDPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTMRPAI 124
           AKDPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTMRPAI
Sbjct: 95  AKDPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTMRPAI 154

Query: 125 LNAVKRAKEVYGDLDIIVTGHSMGGAMAAFCAFDLIVNNKASNVQVITFGQPRIGNAAFA 184
           LNAVKRAKEVYGDLDIIVTGHSMGGAMAAFCAFDLIVNNKASNVQVITFGQPRIGNAAFA
Sbjct: 155 LNAVKRAKEVYGDLDIIVTGHSMGGAMAAFCAFDLIVNNKASNVQVITFGQPRIGNAAFA 214

Query: 185 SHYSKHLPNTIRVTNGHDMVPHLPPYFFIFPRRTYHHFPREVWLQGTSSKCFASYTEIVC 244
           SHYSKHLPNTIRVTNGHDMVPHLPPYFFIFPRRTYHHFPREVWLQGTSSKCFASYTEIVC
Sbjct: 215 SHYSKHLPNTIRVTNGHDMVPHLPPYFFIFPRRTYHHFPREVWLQGTSSKCFASYTEIVC 274

Query: 245 DDSGEDLTCSRSVIGNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVEYGSIDSKGNIMLF 304
           DDSGEDLTCSRSVIGNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVEYGSIDSKGNIMLF
Sbjct: 275 DDSGEDLTCSRSVIGNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVEYGSIDSKGNIMLF 334

Query: 305 RDPATPIPIIPTLEAN 321
           RDPATPIPIIPTLEAN
Sbjct: 335 RDPATPIPIIPTLEAN 350

BLAST of MS001735 vs. ExPASy TrEMBL
Match: A0A6J1EQK1 (lipase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436798 PE=4 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 7.2e-149
Identity = 252/317 (79.50%), Postives = 278/317 (87.70%), Query Frame = 0

Query: 5   SGYHHSQTHPINGFIFQVYISDMTALFTWTCSRCNGFTEGFEVVELVFDVESCLQSFVGV 64
           S Y+H+    +  +   VYISDMT+LFTWTC+RC G TEGFEVV+L+ DVESCLQSFVGV
Sbjct: 39  SVYNHTLAMIMVEYASAVYISDMTSLFTWTCTRCRGLTEGFEVVQLIVDVESCLQSFVGV 98

Query: 65  AKDPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTMRPAI 124
           AKDPRAIIIAFRGTRG+SIQNWIEDLFWKQLDLDYPGM GAKVHSGFYRAYHCTT+RPAI
Sbjct: 99  AKDPRAIIIAFRGTRGTSIQNWIEDLFWKQLDLDYPGMEGAKVHSGFYRAYHCTTIRPAI 158

Query: 125 LNAVKRAKEVYGDLDIIVTGHSMGGAMAAFCAFDLIVNNKASNVQVITFGQPRIGNAAFA 184
           LNAVK AKEVYGDLD IVTGHSMGGA+AAFCA DLIVN+   NVQV+TFGQPRIGNA FA
Sbjct: 159 LNAVKTAKEVYGDLDTIVTGHSMGGAIAAFCALDLIVNHNVPNVQVMTFGQPRIGNAVFA 218

Query: 185 SHYSKHLPNTIRVTNGHDMVPHLPPYFFIFPRRTYHHFPREVWLQGTSSKC--FASYTEI 244
           S+YSKHLP+TIRVT+ HD+VPHLPPYF I PR+TYHHFPREVWLQ TSS     ASY E 
Sbjct: 219 SYYSKHLPDTIRVTHEHDIVPHLPPYFSIIPRKTYHHFPREVWLQDTSSTSNRLASYIET 278

Query: 245 VCDDSGEDLTCSRSVIGNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVEYGSIDSKGNIM 304
           VCDDSGED  CSRSVIGNSIQDHLSYYGVEFPTDDP TCWIVMDP++ EYGSIDS+GN++
Sbjct: 279 VCDDSGEDPNCSRSVIGNSIQDHLSYYGVEFPTDDPATCWIVMDPVIAEYGSIDSEGNVV 338

Query: 305 LFRDPATPIPIIPTLEA 320
           LFRD ATP+P + +LEA
Sbjct: 339 LFRDLATPVPRMQSLEA 355

BLAST of MS001735 vs. ExPASy TrEMBL
Match: A0A6J1KBZ9 (lipase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111494218 PE=4 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 6.1e-148
Identity = 251/317 (79.18%), Postives = 278/317 (87.70%), Query Frame = 0

Query: 5   SGYHHSQTHPINGFIFQVYISDMTALFTWTCSRCNGFTEGFEVVELVFDVESCLQSFVGV 64
           S Y+H+    +  +   VYISDMT+LFTWTC+RC G TEGFEVV+L+ DVESCLQSFVGV
Sbjct: 42  SVYNHTLAMIMVEYASAVYISDMTSLFTWTCTRCRGLTEGFEVVQLIVDVESCLQSFVGV 101

Query: 65  AKDPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTMRPAI 124
           AKDPRAIIIAFRGTRG+SIQNWIEDLFWKQLDLDYPGM GAKVHSGFYRAYHCTT+RPAI
Sbjct: 102 AKDPRAIIIAFRGTRGTSIQNWIEDLFWKQLDLDYPGMEGAKVHSGFYRAYHCTTIRPAI 161

Query: 125 LNAVKRAKEVYGDLDIIVTGHSMGGAMAAFCAFDLIVNNKASNVQVITFGQPRIGNAAFA 184
           LNAVK AKEVYGDLD IVTGHSMGGA+AAFCA DLIVN+   NV+V+TFGQPRIGNA FA
Sbjct: 162 LNAVKTAKEVYGDLDTIVTGHSMGGAIAAFCALDLIVNHNVPNVRVMTFGQPRIGNAVFA 221

Query: 185 SHYSKHLPNTIRVTNGHDMVPHLPPYFFIFPRRTYHHFPREVWLQGTSSKC--FASYTEI 244
           S+YSKHLP+TIRVT+ HD+VPHLPPYF I PR+TYHHFPREVWLQ TSS     ASY E 
Sbjct: 222 SYYSKHLPDTIRVTHEHDIVPHLPPYFSIIPRKTYHHFPREVWLQDTSSTSNRLASYIET 281

Query: 245 VCDDSGEDLTCSRSVIGNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVEYGSIDSKGNIM 304
           VCDDSGED  CSRSVIGNSIQDHLSYYGVEFPTDDP TCWIVMDP++ EYGSIDS+GN++
Sbjct: 282 VCDDSGEDPNCSRSVIGNSIQDHLSYYGVEFPTDDPVTCWIVMDPVMAEYGSIDSEGNVV 341

Query: 305 LFRDPATPIPIIPTLEA 320
           LFRD ATP+P + +LEA
Sbjct: 342 LFRDLATPVPRMRSLEA 358

BLAST of MS001735 vs. ExPASy TrEMBL
Match: A0A0A0KBG1 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G073490 PE=4 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 6.8e-147
Identity = 246/307 (80.13%), Postives = 274/307 (89.25%), Query Frame = 0

Query: 7   YHHSQTHPINGFIFQVYISDMTALFTWTCSRCNGFTEGFEVVELVFDVESCLQSFVGVAK 66
           Y+H+    +  +   VYISDMTALFTWTCSRC+G T+GFEVV+LV DVESCLQS+VGVAK
Sbjct: 11  YNHTFAIVMVEYASAVYISDMTALFTWTCSRCHGLTQGFEVVQLVVDVESCLQSYVGVAK 70

Query: 67  DPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTMRPAILN 126
           DP+AIIIAFRGTRG+SIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTT+RPAILN
Sbjct: 71  DPQAIIIAFRGTRGTSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTIRPAILN 130

Query: 127 AVKRAKEVYGDLDIIVTGHSMGGAMAAFCAFDLIVNNKASNVQVITFGQPRIGNAAFASH 186
           AVK+AKE YGDLDIIVTGHSMGGA+AAFCA DLIVN+ A NVQV+TFGQPRIGNAAFAS+
Sbjct: 131 AVKKAKEAYGDLDIIVTGHSMGGAIAAFCALDLIVNHNAPNVQVVTFGQPRIGNAAFASY 190

Query: 187 YSKHLPNTIRVTNGHDMVPHLPPYFFIFPRRTYHHFPREVWLQGTSS--KCFASYTEIVC 246
           Y KHLP T RVT+GHD+VPHLPPYF +  R+TYHHFPREVWL  T+S  KC A   E VC
Sbjct: 191 YGKHLPKTTRVTHGHDIVPHLPPYFSMLRRKTYHHFPREVWLHDTTSKLKCLAYNYETVC 250

Query: 247 DDSGEDLTCSRSVIGNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVEYGSIDSKGNIMLF 306
           DDSGED  CSRSV+GNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLV+YGSIDS+GN++L 
Sbjct: 251 DDSGEDPDCSRSVVGNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVKYGSIDSEGNVVLL 310

Query: 307 RDPATPI 312
           ++PATPI
Sbjct: 311 KNPATPI 317

BLAST of MS001735 vs. ExPASy TrEMBL
Match: A0A5A7SSM7 (Lipase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G001240 PE=4 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.2e-146
Identity = 245/307 (79.80%), Postives = 275/307 (89.58%), Query Frame = 0

Query: 7   YHHSQTHPINGFIFQVYISDMTALFTWTCSRCNGFTEGFEVVELVFDVESCLQSFVGVAK 66
           Y+H+    +  +   VYISDMTALFTWTCSRC+G TEGFEVV+LV DVESCLQS+VGVAK
Sbjct: 41  YNHTFALVMVEYAAAVYISDMTALFTWTCSRCHGLTEGFEVVQLVVDVESCLQSYVGVAK 100

Query: 67  DPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTMRPAILN 126
           DP+A+IIAFRGTRG+SIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTT+RPAILN
Sbjct: 101 DPQAVIIAFRGTRGTSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTIRPAILN 160

Query: 127 AVKRAKEVYGDLDIIVTGHSMGGAMAAFCAFDLIVNNKASNVQVITFGQPRIGNAAFASH 186
           AVK+AKE YGDLDIIVTGHSMGGA+AAFCA DLIVN+ A+NVQV+TFGQPRIGNAAFAS+
Sbjct: 161 AVKKAKEAYGDLDIIVTGHSMGGAIAAFCALDLIVNHNATNVQVVTFGQPRIGNAAFASY 220

Query: 187 YSKHLPNTIRVTNGHDMVPHLPPYFFIFPRRTYHHFPREVWLQGTSS--KCFASYTEIVC 246
           YSKHLP T RVT+GHD+VPHLPPYF +  R+TYHHFP EVWLQ T+S  KC A   E VC
Sbjct: 221 YSKHLPKTTRVTHGHDIVPHLPPYFSMLRRKTYHHFPTEVWLQDTTSKLKCLAHNFETVC 280

Query: 247 DDSGEDLTCSRSVIGNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVEYGSIDSKGNIMLF 306
           DDSGED  CSRSV+GNSIQDHLSYYGV+FPTDDPGTCWIVMDPLLVEY SIDS+GN++L 
Sbjct: 281 DDSGEDPDCSRSVVGNSIQDHLSYYGVDFPTDDPGTCWIVMDPLLVEYESIDSEGNVVLL 340

Query: 307 RDPATPI 312
           ++P+TPI
Sbjct: 341 KNPSTPI 347

BLAST of MS001735 vs. TAIR 10
Match: AT5G18630.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 391.3 bits (1004), Expect = 7.2e-109
Identity = 183/301 (60.80%), Postives = 228/301 (75.75%), Query Frame = 0

Query: 7   YHHSQTHPINGFIFQVYISDMTALFTWTCSRCNGFTEGFEVVELVFDVESCLQSFVGVAK 66
           Y+H+    +  +   VY SD+T LFTWTC RCNG T+ FEV+E++FDVE CLQ++VGVAK
Sbjct: 36  YNHTLAVTLVEYASAVYESDLTKLFTWTCERCNGLTKDFEVIEVIFDVEHCLQAYVGVAK 95

Query: 67  DPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTMRPAILN 126
           D  AIIIAFRGT+  SIQNW+ DLFWKQLDL+YP MP A VH GFY AYH TT+RPA+L+
Sbjct: 96  DLNAIIIAFRGTQEHSIQNWVSDLFWKQLDLNYPDMPDAMVHHGFYSAYHNTTLRPAVLD 155

Query: 127 AVKRAKEVYG-DLDIIVTGHSMGGAMAAFCAFDLIVNNKASNVQVITFGQPRIGNAAFAS 186
           A+ R K+VYG +++IIVTGHSMGGAMA+FC  DL+VN    NVQV+TFGQPR+GNAAFAS
Sbjct: 156 AITRVKKVYGANINIIVTGHSMGGAMASFCGLDLVVNEGEENVQVMTFGQPRVGNAAFAS 215

Query: 187 HYSKHLPNTIRVTNGHDMVPHLPPYFFIFPRRTYHHFPREVWLQGTSSKCFASY-TEIVC 246
           +YS  +PNT R+T+  DMVPHLPPY++ FP++TYHHFP EVW++  S   F  +  E VC
Sbjct: 216 YYSLLVPNTFRITHDRDMVPHLPPYYYHFPQKTYHHFPTEVWVKDFSFSNFVLFGLEKVC 275

Query: 247 DDSGEDLTCSRSVIGNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVEYGSIDSKGNIMLF 306
           D++GED TCSRSV GNSI DHL Y+GVE   +    C IVM+  +  Y   DS+GN+ L 
Sbjct: 276 DNTGEDPTCSRSVRGNSISDHLRYFGVELKCESWRQCSIVMNQDMERYSRKDSRGNLFLS 335

BLAST of MS001735 vs. TAIR 10
Match: AT5G18630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 391.3 bits (1004), Expect = 7.2e-109
Identity = 183/301 (60.80%), Postives = 228/301 (75.75%), Query Frame = 0

Query: 7   YHHSQTHPINGFIFQVYISDMTALFTWTCSRCNGFTEGFEVVELVFDVESCLQSFVGVAK 66
           Y+H+    +  +   VY SD+T LFTWTC RCNG T+ FEV+E++FDVE CLQ++VGVAK
Sbjct: 37  YNHTLAVTLVEYASAVYESDLTKLFTWTCERCNGLTKDFEVIEVIFDVEHCLQAYVGVAK 96

Query: 67  DPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTMRPAILN 126
           D  AIIIAFRGT+  SIQNW+ DLFWKQLDL+YP MP A VH GFY AYH TT+RPA+L+
Sbjct: 97  DLNAIIIAFRGTQEHSIQNWVSDLFWKQLDLNYPDMPDAMVHHGFYSAYHNTTLRPAVLD 156

Query: 127 AVKRAKEVYG-DLDIIVTGHSMGGAMAAFCAFDLIVNNKASNVQVITFGQPRIGNAAFAS 186
           A+ R K+VYG +++IIVTGHSMGGAMA+FC  DL+VN    NVQV+TFGQPR+GNAAFAS
Sbjct: 157 AITRVKKVYGANINIIVTGHSMGGAMASFCGLDLVVNEGEENVQVMTFGQPRVGNAAFAS 216

Query: 187 HYSKHLPNTIRVTNGHDMVPHLPPYFFIFPRRTYHHFPREVWLQGTSSKCFASY-TEIVC 246
           +YS  +PNT R+T+  DMVPHLPPY++ FP++TYHHFP EVW++  S   F  +  E VC
Sbjct: 217 YYSLLVPNTFRITHDRDMVPHLPPYYYHFPQKTYHHFPTEVWVKDFSFSNFVLFGLEKVC 276

Query: 247 DDSGEDLTCSRSVIGNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVEYGSIDSKGNIMLF 306
           D++GED TCSRSV GNSI DHL Y+GVE   +    C IVM+  +  Y   DS+GN+ L 
Sbjct: 277 DNTGEDPTCSRSVRGNSISDHLRYFGVELKCESWRQCSIVMNQDMERYSRKDSRGNLFLS 336

BLAST of MS001735 vs. TAIR 10
Match: AT5G18640.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 389.0 bits (998), Expect = 3.6e-108
Identity = 186/308 (60.39%), Postives = 232/308 (75.32%), Query Frame = 0

Query: 7   YHHSQTHPINGFIFQVYISDMTALFTWTCSRCNGFTEGFEVVELVFDVESCLQSFVGVAK 66
           Y+H+    +  +   VY+SD++ LFTWTC RCNG T+GFEV+E++ DVE CLQ++VGVAK
Sbjct: 36  YNHTLAITLVEYTSAVYMSDLSELFTWTCERCNGLTKGFEVIEIIVDVEHCLQAYVGVAK 95

Query: 67  DPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTMRPAILN 126
           D  AIIIAFRGT+  SIQNW+ DLFWKQLDL+YP MP A VH GFY AYH TT+RPA+L+
Sbjct: 96  DLNAIIIAFRGTQEHSIQNWVSDLFWKQLDLNYPDMPDAMVHHGFYSAYHNTTVRPAVLD 155

Query: 127 AVKRAKEVYG-DLDIIVTGHSMGGAMAAFCAFDLIVNNKASNVQVITFGQPRIGNAAFAS 186
           AVKRAKE YG +L+I+VTGHSMGGAMA+FCA DL+VN    NVQV+TFGQPR+GNAAFAS
Sbjct: 156 AVKRAKESYGANLNIMVTGHSMGGAMASFCALDLVVNEGEENVQVMTFGQPRVGNAAFAS 215

Query: 187 HYSKHLPNTIRVTNGHDMVPHLPPYFFIFPRRTYHHFPREVWLQGTS-SKCFASYTEIVC 246
           +++  +PNT R+ +  D+VPHLPPY+ +FP++TYHHFP EVWL   S         E VC
Sbjct: 216 YFNLLVPNTFRIIHDRDIVPHLPPYYHLFPQKTYHHFPTEVWLTELSILNIVIRGVEKVC 275

Query: 247 DDSGEDLTCSRSVIGNSIQDHLSYYGVEFPTDDPGTCWIVMDPLLVEYGSIDSKGNIMLF 306
           D++GED TCSRSV+GNSI DHL+Y+GVE   +    C IVM   +  Y   DSKGNI L 
Sbjct: 276 DNTGEDPTCSRSVMGNSISDHLTYFGVELMGETWRQCNIVMGHEMESYSRKDSKGNIFLS 335

Query: 307 RD-PATPI 312
           R  P+T +
Sbjct: 336 RTVPSTEV 343

BLAST of MS001735 vs. TAIR 10
Match: AT5G18630.3 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 345.9 bits (886), Expect = 3.5e-95
Identity = 159/251 (63.35%), Postives = 198/251 (78.88%), Query Frame = 0

Query: 7   YHHSQTHPINGFIFQVYISDMTALFTWTCSRCNGFTEGFEVVELVFDVESCLQSFVGVAK 66
           Y+H+    +  +   VY SD+T LFTWTC RCNG T+ FEV+E++FDVE CLQ++VGVAK
Sbjct: 36  YNHTLAVTLVEYASAVYESDLTKLFTWTCERCNGLTKDFEVIEVIFDVEHCLQAYVGVAK 95

Query: 67  DPRAIIIAFRGTRGSSIQNWIEDLFWKQLDLDYPGMPGAKVHSGFYRAYHCTTMRPAILN 126
           D  AIIIAFRGT+  SIQNW+ DLFWKQLDL+YP MP A VH GFY AYH TT+RPA+L+
Sbjct: 96  DLNAIIIAFRGTQEHSIQNWVSDLFWKQLDLNYPDMPDAMVHHGFYSAYHNTTLRPAVLD 155

Query: 127 AVKRAKEVYG-DLDIIVTGHSMGGAMAAFCAFDLIVNNKASNVQVITFGQPRIGNAAFAS 186
           A+ R K+VYG +++IIVTGHSMGGAMA+FC  DL+VN    NVQV+TFGQPR+GNAAFAS
Sbjct: 156 AITRVKKVYGANINIIVTGHSMGGAMASFCGLDLVVNEGEENVQVMTFGQPRVGNAAFAS 215

Query: 187 HYSKHLPNTIRVTNGHDMVPHLPPYFFIFPRRTYHHFPREVWLQGTSSKCFASY-TEIVC 246
           +YS  +PNT R+T+  DMVPHLPPY++ FP++TYHHFP EVW++  S   F  +  E VC
Sbjct: 216 YYSLLVPNTFRITHDRDMVPHLPPYYYHFPQKTYHHFPTEVWVKDFSFSNFVLFGLEKVC 275

Query: 247 DDSGEDLTCSR 256
           D++GED TCSR
Sbjct: 276 DNTGEDPTCSR 286

BLAST of MS001735 vs. TAIR 10
Match: AT2G30550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 77.0 bits (188), Expect = 3.0e-14
Identity = 62/169 (36.69%), Postives = 79/169 (46.75%), Query Frame = 0

Query: 69  RAIIIAFRGTRGSSIQNWIEDL--FWKQL---DLDYPGMPGAKVHSGFYRAY-----HCT 128
           R I IA+RGT   +   WI DL  + K +    +  P  P  KV SGF   Y      C 
Sbjct: 225 RDIAIAWRGT--VTKLEWIADLKDYLKPVTENKIRCPD-PAVKVESGFLDLYTDKDTTCK 284

Query: 129 ----TMRPAILNAVKRAKEVYG-----DLDIIVTGHSMGGAMAAFCAFDLI-------VN 188
               + R  IL  VKR  E +G     DL I VTGHS+GGA+A   A+D+          
Sbjct: 285 FARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKK 344

Query: 189 NKASNVQVITFGQPRIGNAAFASHYSKHLPNTIRVTNGHDMVPHLPPYF 212
            K   V V+T+G PR+GN  F     +     +RV N HD+VP  P  F
Sbjct: 345 GKVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLF 390

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138651.14.8e-17995.57lipase-like isoform X1 [Momordica charantia] >XP_022138654.1 lipase-like isoform... [more]
XP_038875858.11.6e-15080.95lipase-like isoform X1 [Benincasa hispida][more]
XP_038875859.11.6e-15080.95lipase-like isoform X2 [Benincasa hispida][more]
XP_022930304.11.5e-14879.50lipase-like isoform X1 [Cucurbita moschata] >XP_022930305.1 lipase-like isoform ... [more]
KAG6593948.12.5e-14879.50hypothetical protein SDJN03_13424, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
P195159.4e-2935.34Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2[more]
Q9XTR88.0e-2029.52Lipase ZK262.3 OS=Caenorhabditis elegans OX=6239 GN=ZK262.3 PE=1 SV=1[more]
B8NIB81.7e-1728.70Probable feruloyl esterase A OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1... [more]
Q2UNW52.2e-1728.70Probable feruloyl esterase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) ... [more]
O599522.4e-1631.40Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CAB92.3e-17995.57lipase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009758 PE=4 SV=1[more]
A0A6J1EQK17.2e-14979.50lipase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436798 PE=4 SV=1[more]
A0A6J1KBZ96.1e-14879.18lipase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111494218 PE=4 SV=1[more]
A0A0A0KBG16.8e-14780.13Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G073490 PE... [more]
A0A5A7SSM71.2e-14679.80Lipase-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1... [more]
Match NameE-valueIdentityDescription
AT5G18630.27.2e-10960.80alpha/beta-Hydrolases superfamily protein [more]
AT5G18630.17.2e-10960.80alpha/beta-Hydrolases superfamily protein [more]
AT5G18640.13.6e-10860.39alpha/beta-Hydrolases superfamily protein [more]
AT5G18630.33.5e-9563.35alpha/beta-Hydrolases superfamily protein [more]
AT2G30550.13.0e-1436.69alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 7..275
e-value: 3.5E-69
score: 234.9
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 21..273
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 72..210
e-value: 3.7E-37
score: 127.3
NoneNo IPR availablePANTHERPTHR45856ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 7..308
NoneNo IPR availablePANTHERPTHR45856:SF12LIPASE-LIKEcoord: 7..308
NoneNo IPR availableCDDcd00519Lipase_3coord: 49..228
e-value: 4.05929E-62
score: 195.388

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS001735.1MS001735.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process