MS001728 (gene) Bitter gourd (TR) v1

Overview
NameMS001728
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionJ domain-containing protein
Locationscaffold571: 514938 .. 517769 (-)
RNA-Seq ExpressionMS001728
SyntenyMS001728
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTGCAACAAAGAAGAAGCCATTAGAGCAAGGGATATTGCTGAGAAGAAGATGGAAAGCAAAGATTTCACTGGGGCTCGTAAATTCATTCTCAGGGCTCAACAGCTAAATCCTGATGCGGAGAATATTTCACAAATGCTTATGGTCTGTGATGTGCATTGCGCAGCAGAGAAGAAACTGCTTGGGAATGAGATGGACTGGTACGGCATCCTTCAGATTGAGCAAACGGCAAATGAGGCGACAATCAGGAAGCAATACCGGAAGTATGCTCTTCTTCTCCATCCGGACAAAAACAAATTTATTGGTGCAGAAGCTGCTTTCAAGTTGGTCGGTGAAGCTCAAAGAGTGTTGTTGGACCAAGAGAAACGCCGGTTGCATGACATGAGATGCAAACCAGCAGTACCTTATCGACCTCCACAAAGAGCTGGTTCGACTTTTAATGTTGGGGTTCAAGCTAACTTCAGAGGCAACTTTACGGCCTTCACTCCTCAACATCCACAGCCTCAACCCCAAGGACATTCGGGTTTTGGTCAAAACCGTGCAACTTTTTGGACCGTCTGCCCATTTTGTTCAGTGAGGTACCAGTACTATAAAGAAGTCGTCAATAGATCGCTTTGCTGTCAGAATTGCAAGAAGCCCTTTGTTGCATATGATATGGAACTGCAAGGGGCTCACCCTCAGCCTATGTCTAACTTGCACCACACAACATTTTTTCAGCAGCAGAATTCCTTCAATCACAGGGCTGAGATGGGACACCCAGGAAACTCTCAGTCTGAGAAGCGTAGAATGGAATCTCTCCGGAGGACGCACAACACTTCAGGTGCTGCTTCAGAAAAGTTATATGGTAAGAAAAGAAGGAAGCAGACATCTGAATCCAGTGAGAGTTGTGATACTGGAAGTAGCTCCGATTTTGAAGAAAATGTAGTTACTAATGAAGATAGCAAAAAGGATGTTGGACGCCCTGGAGCGCACCATCCACGGAGATCTAGTCGACAGAGGCATAAGATTTCTTACAATGAGAATTTAAGTGACGACGACTCTGTGATCACTCCAAAAAAATCAAAGAGAAGCAAATCGTCCGACAGCTCAGATGAGGACAGTGAAGAAGTATATGTAGACGAGGCATCTAAAATCAATAATCAATCTGGATCTGCTGATGTGGATGATGATCAGAGGGAAACAAATAAGCTAGGACATAATTGCTCTGAAGCGAGCTTATCGCGTAGAAGCAAGGGTGGTAAGAAGGTGAGCCATAAAGAAACATTAGATAACGATTATTCTCAGAGAAGCATGGGGTTAGCTGGTGATCCCGAGGTTAATTTGTTCTCATGTTCTGATCCTGATTTCAATGATTTTGATAAACTACGGAACCGAGAGTGTTTCAAACTTGGGCAGATCTGGGCTATGTATGATAATATTGATACTATGCCTAGATTCTATGCTCGGGTCAAGAAAGTGTTTCCTTCTGGATTCAAGGTTCAGATCACCTGGCTAGAGCCAGAAGCTAATGATGAGTGCCAAAGCAAATGTGTTGATGAAAAAGTGCCAGTTTCTTGTGGTGAATTTGTATCTGGAGCAACTGAGACCATGACAGATTGTGGCTCGATGTTTTCTCATGCTGTTTCTTTGGTCAAAGGCGGCCGTAAAGATATTTTCAAAATTTATCCTAGGAAAGGGCAAATTTGGGCTCTCTTCAAAAACTGGGATAAGAATTACAACCGTGATTCCAATTGCCAGTATGAGTATGAGTTTGTTGAGACATTGTCAGAATATAGCAAAGAATCTGGTATAGATGTTGCACTCCTAGCCAAGGTGAAAGGCTTTTCGTGTCTCTTTTGTCGAATGGTCAAGGAAGGAGAAAATTCGTTTAGAGTCCCTGCAGCTGAATTATTCAGGTTCTCCCACCAGGTTCCTTCGTTTCCACTCACAGGCGATGAAAGAGATGATGTTCCGGAAGGTTCTTTTGAACTTGATCCAGCCGCTCTTCCCCCCAATGTTCCGGAGATCGCCATCCCCCAGCATTTAAAGAAGGTGGCTGCCAATGCTGGTCTTAACACATTGAAGTCCACGGCGGGGCCTAACGGAGATGCAGGCACACACAAAGCCGATGCTGATACTAAAAGCGACGATGGTGATGTGGCTGAGGCTTATGAGATTCCAGACCCTGAATTCCACAACTTCGATGTTGATAAAACTTCTGAGAAGTTTCGGGTTGGTCAGGTCTGGGCATTGTACAGTGATGAGGATGCCTTACCGAAGTATTATGCTCAGATCAAGAAAATAACTCACAAACCGGAGTTCGAATTGAGATTGTTGTGGCTGGAGTCCTCTGAACTGCCAGACGATACTATTGAATGGCACGATAAAAGTATGCCGATTTCTTGTGGGAGATTTAGAACTCAGAGGACTAAGATGCACAGCTACTACTCCACCAAATCCTTTTCTCATCTGGTTAGAACTATTCCTGTTCCTAAGAATGGGTTCTACATCTCTCCTTCATTGGGTGAGGTTTGGGCATTATACAAGAACTGGACCCCTGAAATCAGATGTTCAGATATGGATAAATGTGAATATGTTATAGTAGAAGTCATTGAGGACGACGATTTGCAAAAAGAAGTCTTGGTCTTGGACCGCGTCCATGGTTTCAACTCGGTCTTCAAGGCTAAGACCAAAGGTGATTCTTCAACCGTCACTATGGTAATTACCCGAGTCGAGCTTCTACGATTTTCCCACCGGATTCCCGCTTTTCGGCTGACAGATGAGAGGGGCGGTAGCTTGAGAGGTTGCTTGGAGCTTGATCCTGCCGCTTTACCGATATATTTTTTTTAT

mRNA sequence

ATGGATTGCAACAAAGAAGAAGCCATTAGAGCAAGGGATATTGCTGAGAAGAAGATGGAAAGCAAAGATTTCACTGGGGCTCGTAAATTCATTCTCAGGGCTCAACAGCTAAATCCTGATGCGGAGAATATTTCACAAATGCTTATGGTCTGTGATGTGCATTGCGCAGCAGAGAAGAAACTGCTTGGGAATGAGATGGACTGGTACGGCATCCTTCAGATTGAGCAAACGGCAAATGAGGCGACAATCAGGAAGCAATACCGGAAGTATGCTCTTCTTCTCCATCCGGACAAAAACAAATTTATTGGTGCAGAAGCTGCTTTCAAGTTGGTCGGTGAAGCTCAAAGAGTGTTGTTGGACCAAGAGAAACGCCGGTTGCATGACATGAGATGCAAACCAGCAGTACCTTATCGACCTCCACAAAGAGCTGGTTCGACTTTTAATGTTGGGGTTCAAGCTAACTTCAGAGGCAACTTTACGGCCTTCACTCCTCAACATCCACAGCCTCAACCCCAAGGACATTCGGGTTTTGGTCAAAACCGTGCAACTTTTTGGACCGTCTGCCCATTTTGTTCAGTGAGGTACCAGTACTATAAAGAAGTCGTCAATAGATCGCTTTGCTGTCAGAATTGCAAGAAGCCCTTTGTTGCATATGATATGGAACTGCAAGGGGCTCACCCTCAGCCTATGTCTAACTTGCACCACACAACATTTTTTCAGCAGCAGAATTCCTTCAATCACAGGGCTGAGATGGGACACCCAGGAAACTCTCAGTCTGAGAAGCGTAGAATGGAATCTCTCCGGAGGACGCACAACACTTCAGGTGCTGCTTCAGAAAAGTTATATGGTAAGAAAAGAAGGAAGCAGACATCTGAATCCAGTGAGAGTTGTGATACTGGAAGTAGCTCCGATTTTGAAGAAAATGTAGTTACTAATGAAGATAGCAAAAAGGATGTTGGACGCCCTGGAGCGCACCATCCACGGAGATCTAGTCGACAGAGGCATAAGATTTCTTACAATGAGAATTTAAGTGACGACGACTCTGTGATCACTCCAAAAAAATCAAAGAGAAGCAAATCGTCCGACAGCTCAGATGAGGACAGTGAAGAAGTATATGTAGACGAGGCATCTAAAATCAATAATCAATCTGGATCTGCTGATGTGGATGATGATCAGAGGGAAACAAATAAGCTAGGACATAATTGCTCTGAAGCGAGCTTATCGCGTAGAAGCAAGGGTGGTAAGAAGGTGAGCCATAAAGAAACATTAGATAACGATTATTCTCAGAGAAGCATGGGGTTAGCTGGTGATCCCGAGGTTAATTTGTTCTCATGTTCTGATCCTGATTTCAATGATTTTGATAAACTACGGAACCGAGAGTGTTTCAAACTTGGGCAGATCTGGGCTATGTATGATAATATTGATACTATGCCTAGATTCTATGCTCGGGTCAAGAAAGTGTTTCCTTCTGGATTCAAGGTTCAGATCACCTGGCTAGAGCCAGAAGCTAATGATGAGTGCCAAAGCAAATGTGTTGATGAAAAAGTGCCAGTTTCTTGTGGTGAATTTGTATCTGGAGCAACTGAGACCATGACAGATTGTGGCTCGATGTTTTCTCATGCTGTTTCTTTGGTCAAAGGCGGCCGTAAAGATATTTTCAAAATTTATCCTAGGAAAGGGCAAATTTGGGCTCTCTTCAAAAACTGGGATAAGAATTACAACCGTGATTCCAATTGCCAGTATGAGTATGAGTTTGTTGAGACATTGTCAGAATATAGCAAAGAATCTGGTATAGATGTTGCACTCCTAGCCAAGGTGAAAGGCTTTTCGTGTCTCTTTTGTCGAATGGTCAAGGAAGGAGAAAATTCGTTTAGAGTCCCTGCAGCTGAATTATTCAGGTTCTCCCACCAGGTTCCTTCGTTTCCACTCACAGGCGATGAAAGAGATGATGTTCCGGAAGGTTCTTTTGAACTTGATCCAGCCGCTCTTCCCCCCAATGTTCCGGAGATCGCCATCCCCCAGCATTTAAAGAAGGTGGCTGCCAATGCTGGTCTTAACACATTGAAGTCCACGGCGGGGCCTAACGGAGATGCAGGCACACACAAAGCCGATGCTGATACTAAAAGCGACGATGGTGATGTGGCTGAGGCTTATGAGATTCCAGACCCTGAATTCCACAACTTCGATGTTGATAAAACTTCTGAGAAGTTTCGGGTTGGTCAGGTCTGGGCATTGTACAGTGATGAGGATGCCTTACCGAAGTATTATGCTCAGATCAAGAAAATAACTCACAAACCGGAGTTCGAATTGAGATTGTTGTGGCTGGAGTCCTCTGAACTGCCAGACGATACTATTGAATGGCACGATAAAAGTATGCCGATTTCTTGTGGGAGATTTAGAACTCAGAGGACTAAGATGCACAGCTACTACTCCACCAAATCCTTTTCTCATCTGGTTAGAACTATTCCTGTTCCTAAGAATGGGTTCTACATCTCTCCTTCATTGGGTGAGGTTTGGGCATTATACAAGAACTGGACCCCTGAAATCAGATGTTCAGATATGGATAAATGTGAATATGTTATAGTAGAAGTCATTGAGGACGACGATTTGCAAAAAGAAGTCTTGGTCTTGGACCGCGTCCATGGTTTCAACTCGGTCTTCAAGGCTAAGACCAAAGGTGATTCTTCAACCGTCACTATGGTAATTACCCGAGTCGAGCTTCTACGATTTTCCCACCGGATTCCCGCTTTTCGGCTGACAGATGAGAGGGGCGGTAGCTTGAGAGGTTGCTTGGAGCTTGATCCTGCCGCTTTACCGATATATTTTTTTTAT

Coding sequence (CDS)

ATGGATTGCAACAAAGAAGAAGCCATTAGAGCAAGGGATATTGCTGAGAAGAAGATGGAAAGCAAAGATTTCACTGGGGCTCGTAAATTCATTCTCAGGGCTCAACAGCTAAATCCTGATGCGGAGAATATTTCACAAATGCTTATGGTCTGTGATGTGCATTGCGCAGCAGAGAAGAAACTGCTTGGGAATGAGATGGACTGGTACGGCATCCTTCAGATTGAGCAAACGGCAAATGAGGCGACAATCAGGAAGCAATACCGGAAGTATGCTCTTCTTCTCCATCCGGACAAAAACAAATTTATTGGTGCAGAAGCTGCTTTCAAGTTGGTCGGTGAAGCTCAAAGAGTGTTGTTGGACCAAGAGAAACGCCGGTTGCATGACATGAGATGCAAACCAGCAGTACCTTATCGACCTCCACAAAGAGCTGGTTCGACTTTTAATGTTGGGGTTCAAGCTAACTTCAGAGGCAACTTTACGGCCTTCACTCCTCAACATCCACAGCCTCAACCCCAAGGACATTCGGGTTTTGGTCAAAACCGTGCAACTTTTTGGACCGTCTGCCCATTTTGTTCAGTGAGGTACCAGTACTATAAAGAAGTCGTCAATAGATCGCTTTGCTGTCAGAATTGCAAGAAGCCCTTTGTTGCATATGATATGGAACTGCAAGGGGCTCACCCTCAGCCTATGTCTAACTTGCACCACACAACATTTTTTCAGCAGCAGAATTCCTTCAATCACAGGGCTGAGATGGGACACCCAGGAAACTCTCAGTCTGAGAAGCGTAGAATGGAATCTCTCCGGAGGACGCACAACACTTCAGGTGCTGCTTCAGAAAAGTTATATGGTAAGAAAAGAAGGAAGCAGACATCTGAATCCAGTGAGAGTTGTGATACTGGAAGTAGCTCCGATTTTGAAGAAAATGTAGTTACTAATGAAGATAGCAAAAAGGATGTTGGACGCCCTGGAGCGCACCATCCACGGAGATCTAGTCGACAGAGGCATAAGATTTCTTACAATGAGAATTTAAGTGACGACGACTCTGTGATCACTCCAAAAAAATCAAAGAGAAGCAAATCGTCCGACAGCTCAGATGAGGACAGTGAAGAAGTATATGTAGACGAGGCATCTAAAATCAATAATCAATCTGGATCTGCTGATGTGGATGATGATCAGAGGGAAACAAATAAGCTAGGACATAATTGCTCTGAAGCGAGCTTATCGCGTAGAAGCAAGGGTGGTAAGAAGGTGAGCCATAAAGAAACATTAGATAACGATTATTCTCAGAGAAGCATGGGGTTAGCTGGTGATCCCGAGGTTAATTTGTTCTCATGTTCTGATCCTGATTTCAATGATTTTGATAAACTACGGAACCGAGAGTGTTTCAAACTTGGGCAGATCTGGGCTATGTATGATAATATTGATACTATGCCTAGATTCTATGCTCGGGTCAAGAAAGTGTTTCCTTCTGGATTCAAGGTTCAGATCACCTGGCTAGAGCCAGAAGCTAATGATGAGTGCCAAAGCAAATGTGTTGATGAAAAAGTGCCAGTTTCTTGTGGTGAATTTGTATCTGGAGCAACTGAGACCATGACAGATTGTGGCTCGATGTTTTCTCATGCTGTTTCTTTGGTCAAAGGCGGCCGTAAAGATATTTTCAAAATTTATCCTAGGAAAGGGCAAATTTGGGCTCTCTTCAAAAACTGGGATAAGAATTACAACCGTGATTCCAATTGCCAGTATGAGTATGAGTTTGTTGAGACATTGTCAGAATATAGCAAAGAATCTGGTATAGATGTTGCACTCCTAGCCAAGGTGAAAGGCTTTTCGTGTCTCTTTTGTCGAATGGTCAAGGAAGGAGAAAATTCGTTTAGAGTCCCTGCAGCTGAATTATTCAGGTTCTCCCACCAGGTTCCTTCGTTTCCACTCACAGGCGATGAAAGAGATGATGTTCCGGAAGGTTCTTTTGAACTTGATCCAGCCGCTCTTCCCCCCAATGTTCCGGAGATCGCCATCCCCCAGCATTTAAAGAAGGTGGCTGCCAATGCTGGTCTTAACACATTGAAGTCCACGGCGGGGCCTAACGGAGATGCAGGCACACACAAAGCCGATGCTGATACTAAAAGCGACGATGGTGATGTGGCTGAGGCTTATGAGATTCCAGACCCTGAATTCCACAACTTCGATGTTGATAAAACTTCTGAGAAGTTTCGGGTTGGTCAGGTCTGGGCATTGTACAGTGATGAGGATGCCTTACCGAAGTATTATGCTCAGATCAAGAAAATAACTCACAAACCGGAGTTCGAATTGAGATTGTTGTGGCTGGAGTCCTCTGAACTGCCAGACGATACTATTGAATGGCACGATAAAAGTATGCCGATTTCTTGTGGGAGATTTAGAACTCAGAGGACTAAGATGCACAGCTACTACTCCACCAAATCCTTTTCTCATCTGGTTAGAACTATTCCTGTTCCTAAGAATGGGTTCTACATCTCTCCTTCATTGGGTGAGGTTTGGGCATTATACAAGAACTGGACCCCTGAAATCAGATGTTCAGATATGGATAAATGTGAATATGTTATAGTAGAAGTCATTGAGGACGACGATTTGCAAAAAGAAGTCTTGGTCTTGGACCGCGTCCATGGTTTCAACTCGGTCTTCAAGGCTAAGACCAAAGGTGATTCTTCAACCGTCACTATGGTAATTACCCGAGTCGAGCTTCTACGATTTTCCCACCGGATTCCCGCTTTTCGGCTGACAGATGAGAGGGGCGGTAGCTTGAGAGGTTGCTTGGAGCTTGATCCTGCCGCTTTACCGATATATTTTTTTTAT

Protein sequence

MDCNKEEAIRARDIAEKKMESKDFTGARKFILRAQQLNPDAENISQMLMVCDVHCAAEKKLLGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLDQEKRRLHDMRCKPAVPYRPPQRAGSTFNVGVQANFRGNFTAFTPQHPQPQPQGHSGFGQNRATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNLHHTTFFQQQNSFNHRAEMGHPGNSQSEKRRMESLRRTHNTSGAASEKLYGKKRRKQTSESSESCDTGSSSDFEENVVTNEDSKKDVGRPGAHHPRRSSRQRHKISYNENLSDDDSVITPKKSKRSKSSDSSDEDSEEVYVDEASKINNQSGSADVDDDQRETNKLGHNCSEASLSRRSKGGKKVSHKETLDNDYSQRSMGLAGDPEVNLFSCSDPDFNDFDKLRNRECFKLGQIWAMYDNIDTMPRFYARVKKVFPSGFKVQITWLEPEANDECQSKCVDEKVPVSCGEFVSGATETMTDCGSMFSHAVSLVKGGRKDIFKIYPRKGQIWALFKNWDKNYNRDSNCQYEYEFVETLSEYSKESGIDVALLAKVKGFSCLFCRMVKEGENSFRVPAAELFRFSHQVPSFPLTGDERDDVPEGSFELDPAALPPNVPEIAIPQHLKKVAANAGLNTLKSTAGPNGDAGTHKADADTKSDDGDVAEAYEIPDPEFHNFDVDKTSEKFRVGQVWALYSDEDALPKYYAQIKKITHKPEFELRLLWLESSELPDDTIEWHDKSMPISCGRFRTQRTKMHSYYSTKSFSHLVRTIPVPKNGFYISPSLGEVWALYKNWTPEIRCSDMDKCEYVIVEVIEDDDLQKEVLVLDRVHGFNSVFKAKTKGDSSTVTMVITRVELLRFSHRIPAFRLTDERGGSLRGCLELDPAALPIYFFY
Homology
BLAST of MS001728 vs. NCBI nr
Match: XP_022138503.1 (uncharacterized protein LOC111009660 [Momordica charantia] >XP_022138504.1 uncharacterized protein LOC111009660 [Momordica charantia] >XP_022138506.1 uncharacterized protein LOC111009660 [Momordica charantia] >XP_022138507.1 uncharacterized protein LOC111009660 [Momordica charantia])

HSP 1 Score: 1913.7 bits (4956), Expect = 0.0e+00
Identity = 942/944 (99.79%), Postives = 942/944 (99.79%), Query Frame = 0

Query: 1   MDCNKEEAIRARDIAEKKMESKDFTGARKFILRAQQLNPDAENISQMLMVCDVHCAAEKK 60
           MDCNKEEAIRARDIAEKKMESKDFTGARKFILRAQQLNPDAENISQMLMVCDVHCAAEKK
Sbjct: 1   MDCNKEEAIRARDIAEKKMESKDFTGARKFILRAQQLNPDAENISQMLMVCDVHCAAEKK 60

Query: 61  LLGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120
           LLGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD
Sbjct: 61  LLGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120

Query: 121 QEKRRLHDMRCKPAVPYRPPQRAGSTFNVGVQANFRGNFTAFTPQHPQPQPQGHSGFGQN 180
           QEKRRLHDMRCKPAVPYRPPQRAGSTFNVGVQANFRGNFTAFTPQHPQPQPQGHSGFGQN
Sbjct: 121 QEKRRLHDMRCKPAVPYRPPQRAGSTFNVGVQANFRGNFTAFTPQHPQPQPQGHSGFGQN 180

Query: 181 RATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNLHHTTFFQ 240
           RATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNLHHTTFFQ
Sbjct: 181 RATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNLHHTTFFQ 240

Query: 241 QQNSFNHRAEMGHPGNSQSEKRRMESLRRTHNTSGAASEKLYGKKRRKQTSESSESCDTG 300
           QQNSFNHRAEMGHPGNSQSEKRRMESLRRTHNTSGAASEKLYGKKRRKQTSESSESCDTG
Sbjct: 241 QQNSFNHRAEMGHPGNSQSEKRRMESLRRTHNTSGAASEKLYGKKRRKQTSESSESCDTG 300

Query: 301 SSSDFEENVVTNEDSKKDVGRPGAHHPRRSSRQRHKISYNENLSDDDSVITPKKSKRSKS 360
           SSSDFEENVVTNEDSKKDVGRPGAHHPRRSSRQRHKISYNENLSDDDSVITPKKSKRSKS
Sbjct: 301 SSSDFEENVVTNEDSKKDVGRPGAHHPRRSSRQRHKISYNENLSDDDSVITPKKSKRSKS 360

Query: 361 SDSSDEDSEEVYVDEASKINNQSGSADVDDDQRETNKLGHNCSEASLSRRSKGGKKVSHK 420
           SDSSDEDSEEVYVDEASKINNQSGSADVDDDQRETNKLGHNCSEASLSRRSKGGKKVSHK
Sbjct: 361 SDSSDEDSEEVYVDEASKINNQSGSADVDDDQRETNKLGHNCSEASLSRRSKGGKKVSHK 420

Query: 421 ETLDNDYSQRSMGLAGDPEVNLFSCSDPDFNDFDKLRNRECFKLGQIWAMYDNIDTMPRF 480
           ETLDNDYSQRSMG AGDPEVNLFSCSDPDFNDFDKLRNRECFKLGQIWAMYDNIDTMPRF
Sbjct: 421 ETLDNDYSQRSMGSAGDPEVNLFSCSDPDFNDFDKLRNRECFKLGQIWAMYDNIDTMPRF 480

Query: 481 YARVKKVFPSGFKVQITWLEPEANDECQSKCVDEKVPVSCGEFVSGATETMTDCGSMFSH 540
           YARVKKVFPSGFKVQITWLEPEANDECQSKCVDEKVPVSCGEFVSGATETMTDCGSMFSH
Sbjct: 481 YARVKKVFPSGFKVQITWLEPEANDECQSKCVDEKVPVSCGEFVSGATETMTDCGSMFSH 540

Query: 541 AVSLVKGGRKDIFKIYPRKGQIWALFKNWDKNYNRDSNCQYEYEFVETLSEYSKESGIDV 600
           AVSLVKGGRKDIFKIYPRKGQIWALFKNWDKNYNRDSNCQYEYEF ETLSEYSKESGIDV
Sbjct: 541 AVSLVKGGRKDIFKIYPRKGQIWALFKNWDKNYNRDSNCQYEYEFAETLSEYSKESGIDV 600

Query: 601 ALLAKVKGFSCLFCRMVKEGENSFRVPAAELFRFSHQVPSFPLTGDERDDVPEGSFELDP 660
           ALLAKVKGFSCLFCRMVKEGENSFRVPAAELFRFSHQVPSFPLTGDERDDVPEGSFELDP
Sbjct: 601 ALLAKVKGFSCLFCRMVKEGENSFRVPAAELFRFSHQVPSFPLTGDERDDVPEGSFELDP 660

Query: 661 AALPPNVPEIAIPQHLKKVAANAGLNTLKSTAGPNGDAGTHKADADTKSDDGDVAEAYEI 720
           AALPPNVPEIAIPQHLKKVAANAGLNTLKSTAGPNGDAGTHKADADTKSDDGDVAEAYEI
Sbjct: 661 AALPPNVPEIAIPQHLKKVAANAGLNTLKSTAGPNGDAGTHKADADTKSDDGDVAEAYEI 720

Query: 721 PDPEFHNFDVDKTSEKFRVGQVWALYSDEDALPKYYAQIKKITHKPEFELRLLWLESSEL 780
           PDPEFHNFDVDKTSEKFRVGQVWALYSDEDALPKYYAQIKKITHKPEFELRLLWLESSEL
Sbjct: 721 PDPEFHNFDVDKTSEKFRVGQVWALYSDEDALPKYYAQIKKITHKPEFELRLLWLESSEL 780

Query: 781 PDDTIEWHDKSMPISCGRFRTQRTKMHSYYSTKSFSHLVRTIPVPKNGFYISPSLGEVWA 840
           PDDTIEWHDKSMPISCGRFRTQRTKMHSYYSTKSFSHLVRTIPVPKNGFYISPSLGEVWA
Sbjct: 781 PDDTIEWHDKSMPISCGRFRTQRTKMHSYYSTKSFSHLVRTIPVPKNGFYISPSLGEVWA 840

Query: 841 LYKNWTPEIRCSDMDKCEYVIVEVIEDDDLQKEVLVLDRVHGFNSVFKAKTKGDSSTVTM 900
           LYKNWTPEIRCSDMDKCEYVIVEVIEDDDLQKEVLVLDRVHGFNSVFKAKTKGDSSTVTM
Sbjct: 841 LYKNWTPEIRCSDMDKCEYVIVEVIEDDDLQKEVLVLDRVHGFNSVFKAKTKGDSSTVTM 900

Query: 901 VITRVELLRFSHRIPAFRLTDERGGSLRGCLELDPAALPIYFFY 945
           VITRVELLRFSHRIPAFRLTDERGGSLRGCLELDPAALPIYFFY
Sbjct: 901 VITRVELLRFSHRIPAFRLTDERGGSLRGCLELDPAALPIYFFY 944

BLAST of MS001728 vs. NCBI nr
Match: XP_038907114.1 (uncharacterized protein LOC120092937 [Benincasa hispida] >XP_038907115.1 uncharacterized protein LOC120092937 [Benincasa hispida])

HSP 1 Score: 1448.3 bits (3748), Expect = 0.0e+00
Identity = 732/959 (76.33%), Postives = 816/959 (85.09%), Query Frame = 0

Query: 1   MDCNKEEAIRARDIAEKKMESKDFTGARKFILRAQQLNPDAENISQMLMVCDVHCAAEKK 60
           MDCNK+EAI+AR+IAEKKMESKDFTGARK +L+AQQLNPDAE ISQMLMVCDVHCAAEKK
Sbjct: 1   MDCNKDEAIKAREIAEKKMESKDFTGARKLVLKAQQLNPDAEYISQMLMVCDVHCAAEKK 60

Query: 61  LLGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120
           LLGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD
Sbjct: 61  LLGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120

Query: 121 QEKRRLHDMRCKPAVPYRPPQRAGSTFNVGVQANFRGNFTAFTPQH-PQPQPQGHSGFGQ 180
           QEKRR+HDMR KPA+PYRPP RA STFNVGVQANFR NFT+F PQH P PQPQGHSGF  
Sbjct: 121 QEKRRMHDMRRKPAIPYRPPHRAASTFNVGVQANFRSNFTSFIPQHQPPPQPQGHSGFSH 180

Query: 181 NRATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNLHHTTFF 240
           NR+TFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDME+ G H QPMSNL+ T+FF
Sbjct: 181 NRSTFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMEVHGPHSQPMSNLNQTSFF 240

Query: 241 QQQNSFNHRAEMGHPGNSQSEKRRMESLRRTHNTSGAASEKLYGKKRRKQTSESSESCDT 300
           QQQNSFNHRAEMG PGNSQSEKR     RR  NT  AASEK YGKK  KQTSESSESCDT
Sbjct: 241 QQQNSFNHRAEMGRPGNSQSEKR-----RRKCNTPAAASEKFYGKK--KQTSESSESCDT 300

Query: 301 GSSSDFEENVVTNEDSKKDVGRPGAHHPRRSSRQRHKISYNENLSDDDS---VITPKKSK 360
           GSS D +EN VT++DS+KDVGR G HHPRRSSR+R KISYNEN+SDDD+   V+TPKKSK
Sbjct: 301 GSSFDTDEN-VTDDDSRKDVGRSGDHHPRRSSRRRQKISYNENVSDDDNVDFVVTPKKSK 360

Query: 361 RSKSSDSSDEDSEEVYVDEASKINNQSGSADVDDDQRETNKLGHNCSEASLSRRSKGGKK 420
           R +SS  SD+D EEVYVD+ASKIN Q GS  VDDDQ+ETNK  ++ S   LSRR K  KK
Sbjct: 361 RRRSSVISDDDIEEVYVDDASKINKQHGSVAVDDDQKETNKPENHSSGEDLSRRRKRSKK 420

Query: 421 VSHKETLDNDYSQRSMGLAGDPEVNLFSCSDPDFNDFDKLRNRECFKLGQIWAMYDNIDT 480
            SHKET D D SQ SM  AGDPE NL SCSDPDFNDFDKLRNRECF LGQIWA+YD+IDT
Sbjct: 421 ASHKETPDKDVSQGSMESAGDPESNLLSCSDPDFNDFDKLRNRECFALGQIWALYDDIDT 480

Query: 481 MPRFYARVKKVFPSGFKVQITWLEPEANDECQSKCVDEKVPVSCGEFVSGATETMTDCGS 540
           MPRFYA +KKVFPSGFKVQ+TWLEPEA D+ + KCVD+++PVSCG+FV GATETMTDC S
Sbjct: 481 MPRFYAWIKKVFPSGFKVQVTWLEPEAADDGRRKCVDKEMPVSCGDFVFGATETMTDCDS 540

Query: 541 MFSHAVSLVKGGRKDIFKIYPRKGQIWALFKNWDKNYNRDSNCQYEYEFVETLSEYSKES 600
           MFSHAVS  KG  KD FKIYPRKG+IWALFKNWDKN++ D + QYEYEFVETLSE++ E+
Sbjct: 541 MFSHAVSFDKGCHKDSFKIYPRKGEIWALFKNWDKNHDCDPSSQYEYEFVETLSEFTDEA 600

Query: 601 GIDVALLAKVKGFSCLFCRMVKEGENSFRVPAAELFRFSHQVPSFPLTGDERDDVPEGSF 660
           GIDVALLAKVKGFS LFCRMVKEG  SF+VPA ELFRFSH+VPSFPLTGDER+ VP+GSF
Sbjct: 601 GIDVALLAKVKGFSSLFCRMVKEGGKSFQVPATELFRFSHRVPSFPLTGDEREGVPKGSF 660

Query: 661 ELDPAALPPNVPEIAIPQHLKKVAANAGLNTLKSTAGPNGDAGTHKADAD------TKSD 720
           ELDPAALPPNVPEI +P+H K VA++ G +TL    G NGDA  H+A AD      ++SD
Sbjct: 661 ELDPAALPPNVPEIDLPEHTKAVASDTGRSTL-PMMGSNGDAADHEAAADINIDLQSESD 720

Query: 721 DG-----DVAEAYEIPDPEFHNFDVDKTSEKFRVGQVWALYSDEDALPKYYAQIKKITHK 780
           DG       +EAYEIPDPEFHNFD++K+ +KFR+GQVWALYSDEDALP+YY  IKKIT +
Sbjct: 721 DGIAPVASPSEAYEIPDPEFHNFDLEKSFDKFRIGQVWALYSDEDALPRYYGLIKKITRE 780

Query: 781 PEFELRLLWLESSELPDDTIEWHDKSMPISCGRFRTQ-RTKMHSYYSTKSFSHLVRTIPV 840
           P FE++L WL SS LP +TI+WHDK MPISCGRF TQ RT MH   ST+SFSHL++T P 
Sbjct: 781 PGFEVKLTWLVSSTLPGNTIKWHDKYMPISCGRFTTQRRTPMHCCTSTRSFSHLLKTDPA 840

Query: 841 PKNGFYISPSLGEVWALYKNWTPEIRCSDMDKCEYVIVEVIEDDDLQKEVLVLDRVHGFN 900
           P NGF I+P +GEVWALYKNWTPEIRCSD++KC+Y I EVI+DD+LQKEV+ L RV G N
Sbjct: 841 PNNGFSITPRIGEVWALYKNWTPEIRCSDLEKCQYDIAEVIDDDNLQKEVMFLKRVDGHN 900

Query: 901 SVFKAKTKGDSSTVTMVITRVELLRFSHRIPAFRLTDERGGSLRGCLELDPAALPIYFF 944
           SVFKA+TK D ST+TMVI   E LRFSH+IPAFRLT+ERGGSLRGCLELDPAALP+Y+F
Sbjct: 901 SVFKAQTKNDGSTLTMVIPGAEFLRFSHQIPAFRLTEERGGSLRGCLELDPAALPVYYF 950

BLAST of MS001728 vs. NCBI nr
Match: XP_022959199.1 (uncharacterized protein LOC111460259 [Cucurbita moschata])

HSP 1 Score: 1421.4 bits (3678), Expect = 0.0e+00
Identity = 717/952 (75.32%), Postives = 793/952 (83.30%), Query Frame = 0

Query: 1   MDCNKEEAIRARDIAEKKMESKDFTGARKFILRAQQLNPDAENISQMLMVCDVHCAAEKK 60
           MDCNK+EAI+AR+IAEKKMESKDFTGARK +L+AQQLNPDAENISQMLMVCDVHCAAEKK
Sbjct: 1   MDCNKDEAIKAREIAEKKMESKDFTGARKLVLKAQQLNPDAENISQMLMVCDVHCAAEKK 60

Query: 61  LLGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120
           LLGNEMDWY ILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD
Sbjct: 61  LLGNEMDWYAILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120

Query: 121 QEKRRLHDMRCKPAVPYRPPQRAGSTFNVGVQANFRGNFTAFTPQHPQPQPQGHSGFGQN 180
           QEKRRLHDMR +PA+PY+PP RA  +FNVGVQANFR NFT+F PQH  PQ QGHSGF  N
Sbjct: 121 QEKRRLHDMRRRPAIPYQPPHRAAPSFNVGVQANFRSNFTSFIPQHQPPQQQGHSGFSHN 180

Query: 181 RATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNLHHTTFFQ 240
           RATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQG+HPQPMSNL+ T+FFQ
Sbjct: 181 RATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGSHPQPMSNLNQTSFFQ 240

Query: 241 QQNSFNHRAEMGHPGNSQSEKRRMESLRRTHNTSGAASEKLYGKKRRKQTSESSESCDTG 300
           QQNSFNH+AE G PGNSQS+KR     RRT +TSGAASEK  GKK+RKQTSESSES DTG
Sbjct: 241 QQNSFNHKAEAGRPGNSQSDKR-----RRTRSTSGAASEKFTGKKKRKQTSESSESFDTG 300

Query: 301 SSSDFEENVVTNEDSKKDVGRPGAHHPRRSSRQRHKISYNENLSDDDS---VITPKKSKR 360
           +SSD EENVVT+EDS+KDVG  G    RRS R R K+SYNEN+SDDD+   VITPKKSKR
Sbjct: 301 TSSDQEENVVTDEDSRKDVGCSGEQRQRRSVRSRQKVSYNENVSDDDNDDFVITPKKSKR 360

Query: 361 SKSSDSSDEDSEEVYVDEASKINNQSGSADVDDDQRETNKLGHNCSEASLSRRSKGGKKV 420
            KSS  SD+D EEVYVD ASKINN   S D+D D++E NK  ++CSE +LS R+KG KK 
Sbjct: 361 RKSSIVSDDDVEEVYVDGASKINNHYRSVDLDADRKEVNKQENHCSERNLSHRNKGSKKA 420

Query: 421 SHKETLDNDYSQRSMGLAGDPEVNLFSCSDPDFNDFDKLRNRECFKLGQIWAMYDNIDTM 480
           SHKET D D SQ S   AGDP+ N  SCSDPDF+DFDKLRNRECF LGQIWAMYD+IDTM
Sbjct: 421 SHKETADKDVSQGSTESAGDPKCNFLSCSDPDFHDFDKLRNRECFALGQIWAMYDDIDTM 480

Query: 481 PRFYARVKKVFPSGFKVQITWLEPEANDECQSKCVDEKVPVSCGEFVSGATETMTDCGSM 540
           PRFYA +KKVFPSGFKVQITWLEPE  D+ + KCV++++P+SCGEFV GATETMTDCGSM
Sbjct: 481 PRFYAWIKKVFPSGFKVQITWLEPEVTDDGRRKCVNKEMPISCGEFVFGATETMTDCGSM 540

Query: 541 FSHAVSLVKGGRKDIFKIYPRKGQIWALFKNWDKNYNRDSNCQYEYEFVETLSEYSKESG 600
           FSH VS  KG RKD FKIYPRKG+IWALFKNWDKN N DSN +YEYEFVETLSE+++E+G
Sbjct: 541 FSHVVSWDKGCRKDSFKIYPRKGEIWALFKNWDKNSNCDSNSRYEYEFVETLSEFTEEAG 600

Query: 601 IDVALLAKVKGFSCLFCRMVKEGENSFRVPAAELFRFSHQVPSFPLTGDERDDVPEGSFE 660
           IDVA LAKVKGFSCLFCR VKEG    RVPAAELFRFSH++PSFPLTGDER+DVPE S E
Sbjct: 601 IDVAFLAKVKGFSCLFCRTVKEGGKPVRVPAAELFRFSHRIPSFPLTGDEREDVPEDSLE 660

Query: 661 LDPAALPPNVPEIAIPQHLKKVAANAGLNTLKSTAGPNGDAGTHKADADTKSDDGDV--- 720
           LDPAALPPNV EI IP  LK++A  A +                K    ++SDDG+V   
Sbjct: 661 LDPAALPPNVTEIVIPVQLKEMATAADI----------------KTGLRSESDDGNVPVA 720

Query: 721 --AEAYEIPDPEFHNFDVDKTSEKFRVGQVWALYSDEDALPKYYAQIKKITHKPEFELRL 780
              EAYEIPDPEFHNFDVDK+SEKF +GQVWALYSDEDALP+YY  IKKIT +P FE++L
Sbjct: 721 SSGEAYEIPDPEFHNFDVDKSSEKFAIGQVWALYSDEDALPRYYGLIKKITREPRFEVKL 780

Query: 781 LWLESSELPDDTIEWHDKSMPISCGRFRTQ-RTKMHSYYSTKSFSHLVRTIPVPKNGFYI 840
            WLESS LP+DTIEWHDK M I CGRFRTQ RT MH   ST  FSHLVRT   P +GF I
Sbjct: 781 TWLESSSLPNDTIEWHDKGMLIPCGRFRTQRRTTMHCCTSTDPFSHLVRTDSAPNSGFSI 840

Query: 841 SPSLGEVWALYKNWTPEIRCSDMDKCEYVIVEVIEDDDLQKEVLVLDRVHGFNSVFKAKT 900
            P  GEVWALYKNWTPEIR SD++KCEY I EVI+DDD QKEV+VL RV G NS+FKA+T
Sbjct: 841 FPRTGEVWALYKNWTPEIRPSDLEKCEYDIAEVIDDDDSQKEVMVLKRVDGHNSIFKAQT 900

Query: 901 KGDSSTVTMVITRVELLRFSHRIPAFRLTDERGGSLRGCLELDPAALPIYFF 944
           K D ST+TM I + ELLRFSH+IPAFRLTDE GGSLRGCLELDPAALP+Y+F
Sbjct: 901 KNDGSTLTMTIPQAELLRFSHQIPAFRLTDELGGSLRGCLELDPAALPVYYF 931

BLAST of MS001728 vs. NCBI nr
Match: XP_023548310.1 (uncharacterized protein LOC111806985 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1419.1 bits (3672), Expect = 0.0e+00
Identity = 713/947 (75.29%), Postives = 787/947 (83.10%), Query Frame = 0

Query: 1   MDCNKEEAIRARDIAEKKMESKDFTGARKFILRAQQLNPDAENISQMLMVCDVHCAAEKK 60
           MDCNK+EAI+AR+IAEKKMESKDFTGARK +L+AQQLNPDAENISQMLMVCDVHCAAEKK
Sbjct: 1   MDCNKDEAIKAREIAEKKMESKDFTGARKLVLKAQQLNPDAENISQMLMVCDVHCAAEKK 60

Query: 61  LLGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120
           LLGNEMDWY ILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD
Sbjct: 61  LLGNEMDWYAILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120

Query: 121 QEKRRLHDMRCKPAVPYRPPQRAGSTFNVGVQANFRGNFTAFTPQHPQPQPQGHSGFGQN 180
           QEKRRLHDMR +PA+PY+PP RA  +FNVGVQANFR NFT+F PQH  PQ QGHSGF  N
Sbjct: 121 QEKRRLHDMRRRPAIPYQPPHRAAPSFNVGVQANFRSNFTSFIPQHQPPQQQGHSGFSHN 180

Query: 181 RATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNLHHTTFFQ 240
           RATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNL+ T+FFQ
Sbjct: 181 RATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNLNQTSFFQ 240

Query: 241 QQNSFNHRAEMGHPGNSQSEKRRMESLRRTHNTSGAASEKLYGKKRRKQTSESSESCDTG 300
           QQ+SFNH+A+ G PGNSQS+KR     RRT +TS AASEK  GKK+RKQTSESSES DTG
Sbjct: 241 QQSSFNHKADTGRPGNSQSDKR-----RRTRSTSAAASEKFTGKKKRKQTSESSESFDTG 300

Query: 301 SSSDFEENVVTNEDSKKDVGRPGAHHPRRSSRQRHKISYNENLSDDDS---VITPKKSKR 360
           +SSD EENVVT+EDS+KDVG  G    RRS R R K+SYNEN+SDDD+   VITPKKSKR
Sbjct: 301 TSSDQEENVVTDEDSRKDVGCSGEQRQRRSVRSRQKVSYNENVSDDDNDDFVITPKKSKR 360

Query: 361 SKSSDSSDEDSEEVYVDEASKINNQSGSADVDDDQRETNKLGHNCSEASLSRRSKGGKKV 420
            KSS  SD+D EEVYVD ASKINN   S D+D D++E NK  ++CSE +LS R+KG KK 
Sbjct: 361 RKSSIVSDDDVEEVYVDGASKINNHYRSVDLDADRKEVNKQENHCSERNLSNRNKGSKKA 420

Query: 421 SHKETLDNDYSQRSMGLAGDPEVNLFSCSDPDFNDFDKLRNRECFKLGQIWAMYDNIDTM 480
           SHKET D D SQ S   AGDP+ N  SCSDPDF+DFDKLRNRECF LGQIWAMYD+IDTM
Sbjct: 421 SHKETADKDVSQGSTESAGDPKCNFLSCSDPDFHDFDKLRNRECFALGQIWAMYDDIDTM 480

Query: 481 PRFYARVKKVFPSGFKVQITWLEPEANDECQSKCVDEKVPVSCGEFVSGATETMTDCGSM 540
           PRFYA +KKVFPSGFKVQITWLEPE  D+ + KCVD+++P+SCGEFV GATETMTDCGSM
Sbjct: 481 PRFYAWIKKVFPSGFKVQITWLEPEVTDDGRRKCVDKEMPISCGEFVFGATETMTDCGSM 540

Query: 541 FSHAVSLVKGGRKDIFKIYPRKGQIWALFKNWDKNYNRDSNCQYEYEFVETLSEYSKESG 600
           FSH VS  KG RKD FKIYPRKG+IWALFKNWD+N N DSN +YEYEFVETLSE+++E+G
Sbjct: 541 FSHVVSWDKGCRKDSFKIYPRKGEIWALFKNWDENSNCDSNSRYEYEFVETLSEFTEEAG 600

Query: 601 IDVALLAKVKGFSCLFCRMVKEGENSFRVPAAELFRFSHQVPSFPLTGDERDDVPEGSFE 660
           +DVA LAKVKGFSCLFCR VKEG    RVPAAELFRFSH++PSFPLTGDER+DVPE S E
Sbjct: 601 VDVAFLAKVKGFSCLFCRTVKEGGKPVRVPAAELFRFSHRIPSFPLTGDEREDVPEDSLE 660

Query: 661 LDPAALPPNVPEIAIPQHLKKVAANAGLNTLKSTAGPNGDAGTHKADADTKSDDGDVAEA 720
           LDPAALPPNVPEI IP  LK++AA A + T           G      D         EA
Sbjct: 661 LDPAALPPNVPEIVIPVQLKEMAAAADIKT-----------GLRSESDDANVPVASSGEA 720

Query: 721 YEIPDPEFHNFDVDKTSEKFRVGQVWALYSDEDALPKYYAQIKKITHKPEFELRLLWLES 780
           YEIPDPEFHNFDVDK+SEKF +GQVWALYSDEDALP+YY  IKKIT +P FE++L WLES
Sbjct: 721 YEIPDPEFHNFDVDKSSEKFSIGQVWALYSDEDALPRYYGLIKKITREPRFEVKLTWLES 780

Query: 781 SELPDDTIEWHDKSMPISCGRFRTQ-RTKMHSYYSTKSFSHLVRTIPVPKNGFYISPSLG 840
           S LP+DTIEWHDK M I CGRFRTQ RT MH   ST  FSHLVRT   P +GF I P  G
Sbjct: 781 SSLPNDTIEWHDKGMLIPCGRFRTQRRTTMHCCTSTDPFSHLVRTDSAPNSGFSIFPRTG 840

Query: 841 EVWALYKNWTPEIRCSDMDKCEYVIVEVIEDDDLQKEVLVLDRVHGFNSVFKAKTKGDSS 900
           EVWALYKNWTPEIR SD++KCEY I EVI+DDD  KEV+VL RV G NS+FKA+TK D S
Sbjct: 841 EVWALYKNWTPEIRSSDLEKCEYDIAEVIDDDDSHKEVMVLKRVDGHNSIFKAQTKNDGS 900

Query: 901 TVTMVITRVELLRFSHRIPAFRLTDERGGSLRGCLELDPAALPIYFF 944
           T+TM I + ELLRFSH+IPAFRLTDE GGSLRGCLELDPAALPIY+F
Sbjct: 901 TLTMTIPQAELLRFSHQIPAFRLTDELGGSLRGCLELDPAALPIYYF 931

BLAST of MS001728 vs. NCBI nr
Match: KAA0033457.1 (DNAJ heat shock N-terminal domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 1417.9 bits (3669), Expect = 0.0e+00
Identity = 721/960 (75.10%), Postives = 802/960 (83.54%), Query Frame = 0

Query: 1   MDCNKEEAIRARDIAEKKMESKDFTGARKFILRAQQLNPDAENISQMLMVCDVHCAAEKK 60
           MDCNK+EAI+AR+IAEKKM  KDF GARK +L+AQQLNPDAE ISQMLMVCDVHCAAEKK
Sbjct: 1   MDCNKDEAIKAREIAEKKMAGKDFIGARKLVLKAQQLNPDAEYISQMLMVCDVHCAAEKK 60

Query: 61  LLGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120
           L GNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD
Sbjct: 61  LFGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120

Query: 121 QEKRRLHDMRCKPAVPYRPPQRAGSTFNVGVQANFRGNFTAFTPQH-PQPQPQGHSGFGQ 180
            EKRR+HDMR KPA+PYRPP RA STFNVGVQAN+R NFT F PQH P PQPQGHSGF  
Sbjct: 121 HEKRRMHDMRRKPAIPYRPPHRAASTFNVGVQANYRSNFTTFIPQHQPPPQPQGHSGFSH 180

Query: 181 NRATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNLHHTTFF 240
           NR+TFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNL+ T+FF
Sbjct: 181 NRSTFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNLNQTSFF 240

Query: 241 QQQNSFNHRAEMGHPGNSQSEKRRMESLRRTHNTSGAASEKLYGKKRRKQTSESSESCDT 300
           QQQNSFNHR EMG PGNSQSEKR+ +      NTS  ASEK+ GKKRRKQTSESSESCDT
Sbjct: 241 QQQNSFNHRPEMGRPGNSQSEKRQGK-----RNTSVTASEKINGKKRRKQTSESSESCDT 300

Query: 301 GSSSDFEENVVTNED-SKKDVGRPGAHHPRRSSRQRHKISYNENLSDDDS---VITPKKS 360
            SS + +EN VT++D S+KDVG  G H PRRSSR+R KISYNEN SDDD+    +T K+S
Sbjct: 301 ESSLETDENGVTDDDSSRKDVGHSGDHRPRRSSRRRQKISYNENGSDDDNDDIEVTHKRS 360

Query: 361 KRSKSSDSSDEDSEEVYVDEASKINNQSGSADVDDDQRETNKLGHNCSEASLSRRSKGGK 420
           KR KSS  SD+D EEV             S  VDDDQ +TNK  ++CSE  LSRRSKG K
Sbjct: 361 KRRKSSIVSDDDIEEV-------------SVAVDDDQTDTNKPENHCSEEDLSRRSKGSK 420

Query: 421 KVSHKETLDNDYSQRSMGLAGDPEVNLFSCSDPDFNDFDKLRNRECFKLGQIWAMYDNID 480
           K +HKET D D SQ SM  AGDPE NL SCSDPDF+DFD+LRNRECF LGQIWAMYD+ID
Sbjct: 421 KENHKETTDQDVSQGSMESAGDPESNLLSCSDPDFHDFDQLRNRECFALGQIWAMYDDID 480

Query: 481 TMPRFYARVKKVFPSGFKVQITWLEPEANDECQSKCVDEKVPVSCGEFVSGATETMTDCG 540
           TMPRFYA +KKVFPSGFKVQITWLEPEA+ + + KCVD+++PVSCGEFV GATETMTDC 
Sbjct: 481 TMPRFYAWIKKVFPSGFKVQITWLEPEASVDGRRKCVDKEMPVSCGEFVFGATETMTDCD 540

Query: 541 SMFSHAVSLVKGGRKDIFKIYPRKGQIWALFKNWDKNYNRDSNCQYEYEFVETLSEYSKE 600
           SMFSHAVS VKG  KD FKIYPRKG+IWALFKNWDKN   DSN QYEYEFVE LSE+++E
Sbjct: 541 SMFSHAVSWVKGCHKDSFKIYPRKGEIWALFKNWDKNRECDSNGQYEYEFVEILSEFTEE 600

Query: 601 SGIDVALLAKVKGFSCLFCRMVKEGENSFRVPAAELFRFSHQVPSFPLTGDERDDVPEGS 660
           +GIDVALLAKVKGFS LFCRMVK GE S++VPAAELFRFSH+VPSFPLTGDER+DVP GS
Sbjct: 601 AGIDVALLAKVKGFSSLFCRMVKVGEKSYQVPAAELFRFSHRVPSFPLTGDEREDVPRGS 660

Query: 661 FELDPAALPPNVPEIAIPQHLKKVAANAGLNTLKSTAGPNGDAGTHKADADTKS------ 720
           FELDPAALPPN+PEI +P+H+K+VA++   +TL    G NGDAGTH+A  DT S      
Sbjct: 661 FELDPAALPPNLPEIILPEHIKEVASDIRGSTL-PMMGSNGDAGTHEAAGDTNSNLRSEG 720

Query: 721 DDGDVA-----EAYEIPDPEFHNFDVDKTSEKFRVGQVWALYSDEDALPKYYAQIKKITH 780
           DDG  A     EAYEIPDPEFHNFD++K+ EKFR+GQVW+LYSDEDALP+YY  IKK+T 
Sbjct: 721 DDGIAAVASPSEAYEIPDPEFHNFDLEKSIEKFRIGQVWSLYSDEDALPRYYGLIKKVTR 780

Query: 781 KPEFELRLLWLESSELPDDTIEWHDKSMPISCGRFRTQ-RTKMHSYYSTKSFSHLVRTIP 840
           +P FE++L WL SS LP DT++WHDK MPISCGRF TQ RT MH Y S  SFSHL+R  P
Sbjct: 781 EP-FEVKLTWLVSSTLPSDTVKWHDKRMPISCGRFTTQRRTAMHRYTSIDSFSHLLRADP 840

Query: 841 VPKNGFYISPSLGEVWALYKNWTPEIRCSDMDKCEYVIVEVIEDDDLQKEVLVLDRVHGF 900
            P N F ISP +GEVWALYKNWTPEIRCSD+DKCEY I EVI+DDDLQKEV+ L RV G+
Sbjct: 841 APNNAFSISPRIGEVWALYKNWTPEIRCSDLDKCEYDIAEVIDDDDLQKEVMFLKRVDGY 900

Query: 901 NSVFKAKTKGDSSTVTMVITRVELLRFSHRIPAFRLTDERGGSLRGCLELDPAALPIYFF 944
           NSVFKA+TK D ST+TM+IT  E+LRFSH+IPAFRLTDERGGSLRGCLELDPAALP+Y+F
Sbjct: 901 NSVFKAQTKNDGSTLTMLITDAEILRFSHQIPAFRLTDERGGSLRGCLELDPAALPVYYF 940

BLAST of MS001728 vs. ExPASy Swiss-Prot
Match: Q9FH28 (Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2)

HSP 1 Score: 70.5 bits (171), Expect = 1.2e-10
Identity = 56/161 (34.78%), Postives = 76/161 (47.20%), Query Frame = 0

Query: 1   MDCNKEEAIRARDIAEKKMESKDFTGARKFILRAQQLNPDAENISQMLMVCD-------V 60
           MD NK++A R   IAE  + S D   A KFI  A++LNP   ++ +++  CD        
Sbjct: 1   MDGNKDDASRCLRIAEDAIVSGDKERALKFINMAKRLNPSL-SVDELVAACDNLDSVSRN 60

Query: 61  HCAAEK---------KLLGNEM-----------------DWYGILQIEQTANEATIRKQY 120
              +EK         KL   +M                 D+Y IL +E+  +   IRK Y
Sbjct: 61  SSVSEKLKTMDGDDDKLETGKMKYTEENVDLVRNIIRNNDYYAILGLEKNCSVDEIRKAY 120

Query: 121 RKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLDQEKRRLHD 129
           RK +L +HPDKNK  G+E AFK V +A   L D   RR  D
Sbjct: 121 RKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQFD 160

BLAST of MS001728 vs. ExPASy Swiss-Prot
Match: A0A0P0VG31 (DnaJ protein P58IPK homolog A OS=Oryza sativa subsp. japonica OX=39947 GN=P58A PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 4.4e-10
Identity = 46/116 (39.66%), Postives = 65/116 (56.03%), Query Frame = 0

Query: 16  EKKMESKDFTGARKFILRAQQLNPDAENISQMLMVCDVHCAAEKKL-LGNEMDWYGILQI 75
           E K+ ++D+ GA + +  A Q +P    I + LM       AEK+L L    DWY IL I
Sbjct: 311 EAKLLTEDWEGAVQDLKEAAQKSPQDMGIREALM------RAEKQLKLSKRKDWYKILGI 370

Query: 76  EQTANEATIRKQYRKYALLLHPDKN--KFIGAEAAFKLVGEAQRVLLDQEKRRLHD 129
            +TA+ A I++ Y+K AL  HPDKN  K   AE  F+ +  A  VL D++KR  +D
Sbjct: 371 SKTASAAEIKRAYKKLALQWHPDKNVDKREEAENMFREIAAAYEVLGDEDKRVRYD 420

BLAST of MS001728 vs. ExPASy Swiss-Prot
Match: Q5JNB5 (DnaJ protein P58IPK homolog B OS=Oryza sativa subsp. japonica OX=39947 GN=P58B PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.3e-09
Identity = 45/116 (38.79%), Postives = 64/116 (55.17%), Query Frame = 0

Query: 16  EKKMESKDFTGARKFILRAQQLNPDAENISQMLMVCDVHCAAEKKL-LGNEMDWYGILQI 75
           E K+ ++D+ GA + +  A Q +P    I + LM       AEK+L L    DWY IL I
Sbjct: 307 EAKLLTEDWEGAVQDLKEASQKSPQDMGIREALM------RAEKQLKLSKRKDWYKILGI 366

Query: 76  EQTANEATIRKQYRKYALLLHPDKN--KFIGAEAAFKLVGEAQRVLLDQEKRRLHD 129
            +TA+ A I++ Y+K AL  HPDKN      AE  F+ +  A  VL D++KR  +D
Sbjct: 367 SKTASAADIKRAYKKLALQWHPDKNVDNREEAENMFREIAAAYEVLGDEDKRVRYD 416

BLAST of MS001728 vs. ExPASy Swiss-Prot
Match: Q7NBW0 (Chaperone protein DnaJ OS=Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2)) OX=710127 GN=dnaJ PE=3 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 1.7e-09
Identity = 30/67 (44.78%), Postives = 46/67 (68.66%), Query Frame = 0

Query: 62  LGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLDQ 121
           + ++ D+Y IL++ ++A +  I+K +RK A+  HPD+NK   AE  FK V EA  VL D+
Sbjct: 1   MSSKRDYYEILEVSRSATQQDIKKAFRKLAMKYHPDRNKDSDAEEKFKEVNEAYEVLSDE 60

Query: 122 EKRRLHD 129
           EKR+L+D
Sbjct: 61  EKRKLYD 67

BLAST of MS001728 vs. ExPASy Swiss-Prot
Match: Q9QYI4 (DnaJ homolog subfamily B member 12 OS=Mus musculus OX=10090 GN=Dnajb12 PE=1 SV=2)

HSP 1 Score: 64.3 bits (155), Expect = 8.4e-09
Identity = 52/172 (30.23%), Postives = 75/172 (43.60%), Query Frame = 0

Query: 1   MDCNKEEAIRARDIAEKKMESKDFTGARKFILRAQQLNPD------AENISQM------- 60
           M+ NK+EA R   IA K ++S     A +F+ +AQ+L P        E+++Q        
Sbjct: 1   MESNKDEAERCISIALKAIQSNQPERALRFLEKAQRLYPTPRVSALIESLNQKPQSTGDH 60

Query: 61  LMVCDVHCAAEKKLLGNE-------------------------------MDWYGILQIEQ 120
               D      KK  G E                                D+Y IL + +
Sbjct: 61  PQPTDTTHTTTKKAGGTETPSANGEAGGGESAKGYTSEQVAAVKRVKQCKDYYEILGVSR 120

Query: 121 TANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLDQEKRRLHD 129
           +A++  ++K YRK AL  HPDKN   GA  AFK +G A  VL + EKR+ +D
Sbjct: 121 SASDEDLKKAYRKLALKFHPDKNHAPGATEAFKAIGTAYAVLSNPEKRKQYD 172

BLAST of MS001728 vs. ExPASy TrEMBL
Match: A0A6J1C9X2 (uncharacterized protein LOC111009660 OS=Momordica charantia OX=3673 GN=LOC111009660 PE=4 SV=1)

HSP 1 Score: 1913.7 bits (4956), Expect = 0.0e+00
Identity = 942/944 (99.79%), Postives = 942/944 (99.79%), Query Frame = 0

Query: 1   MDCNKEEAIRARDIAEKKMESKDFTGARKFILRAQQLNPDAENISQMLMVCDVHCAAEKK 60
           MDCNKEEAIRARDIAEKKMESKDFTGARKFILRAQQLNPDAENISQMLMVCDVHCAAEKK
Sbjct: 1   MDCNKEEAIRARDIAEKKMESKDFTGARKFILRAQQLNPDAENISQMLMVCDVHCAAEKK 60

Query: 61  LLGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120
           LLGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD
Sbjct: 61  LLGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120

Query: 121 QEKRRLHDMRCKPAVPYRPPQRAGSTFNVGVQANFRGNFTAFTPQHPQPQPQGHSGFGQN 180
           QEKRRLHDMRCKPAVPYRPPQRAGSTFNVGVQANFRGNFTAFTPQHPQPQPQGHSGFGQN
Sbjct: 121 QEKRRLHDMRCKPAVPYRPPQRAGSTFNVGVQANFRGNFTAFTPQHPQPQPQGHSGFGQN 180

Query: 181 RATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNLHHTTFFQ 240
           RATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNLHHTTFFQ
Sbjct: 181 RATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNLHHTTFFQ 240

Query: 241 QQNSFNHRAEMGHPGNSQSEKRRMESLRRTHNTSGAASEKLYGKKRRKQTSESSESCDTG 300
           QQNSFNHRAEMGHPGNSQSEKRRMESLRRTHNTSGAASEKLYGKKRRKQTSESSESCDTG
Sbjct: 241 QQNSFNHRAEMGHPGNSQSEKRRMESLRRTHNTSGAASEKLYGKKRRKQTSESSESCDTG 300

Query: 301 SSSDFEENVVTNEDSKKDVGRPGAHHPRRSSRQRHKISYNENLSDDDSVITPKKSKRSKS 360
           SSSDFEENVVTNEDSKKDVGRPGAHHPRRSSRQRHKISYNENLSDDDSVITPKKSKRSKS
Sbjct: 301 SSSDFEENVVTNEDSKKDVGRPGAHHPRRSSRQRHKISYNENLSDDDSVITPKKSKRSKS 360

Query: 361 SDSSDEDSEEVYVDEASKINNQSGSADVDDDQRETNKLGHNCSEASLSRRSKGGKKVSHK 420
           SDSSDEDSEEVYVDEASKINNQSGSADVDDDQRETNKLGHNCSEASLSRRSKGGKKVSHK
Sbjct: 361 SDSSDEDSEEVYVDEASKINNQSGSADVDDDQRETNKLGHNCSEASLSRRSKGGKKVSHK 420

Query: 421 ETLDNDYSQRSMGLAGDPEVNLFSCSDPDFNDFDKLRNRECFKLGQIWAMYDNIDTMPRF 480
           ETLDNDYSQRSMG AGDPEVNLFSCSDPDFNDFDKLRNRECFKLGQIWAMYDNIDTMPRF
Sbjct: 421 ETLDNDYSQRSMGSAGDPEVNLFSCSDPDFNDFDKLRNRECFKLGQIWAMYDNIDTMPRF 480

Query: 481 YARVKKVFPSGFKVQITWLEPEANDECQSKCVDEKVPVSCGEFVSGATETMTDCGSMFSH 540
           YARVKKVFPSGFKVQITWLEPEANDECQSKCVDEKVPVSCGEFVSGATETMTDCGSMFSH
Sbjct: 481 YARVKKVFPSGFKVQITWLEPEANDECQSKCVDEKVPVSCGEFVSGATETMTDCGSMFSH 540

Query: 541 AVSLVKGGRKDIFKIYPRKGQIWALFKNWDKNYNRDSNCQYEYEFVETLSEYSKESGIDV 600
           AVSLVKGGRKDIFKIYPRKGQIWALFKNWDKNYNRDSNCQYEYEF ETLSEYSKESGIDV
Sbjct: 541 AVSLVKGGRKDIFKIYPRKGQIWALFKNWDKNYNRDSNCQYEYEFAETLSEYSKESGIDV 600

Query: 601 ALLAKVKGFSCLFCRMVKEGENSFRVPAAELFRFSHQVPSFPLTGDERDDVPEGSFELDP 660
           ALLAKVKGFSCLFCRMVKEGENSFRVPAAELFRFSHQVPSFPLTGDERDDVPEGSFELDP
Sbjct: 601 ALLAKVKGFSCLFCRMVKEGENSFRVPAAELFRFSHQVPSFPLTGDERDDVPEGSFELDP 660

Query: 661 AALPPNVPEIAIPQHLKKVAANAGLNTLKSTAGPNGDAGTHKADADTKSDDGDVAEAYEI 720
           AALPPNVPEIAIPQHLKKVAANAGLNTLKSTAGPNGDAGTHKADADTKSDDGDVAEAYEI
Sbjct: 661 AALPPNVPEIAIPQHLKKVAANAGLNTLKSTAGPNGDAGTHKADADTKSDDGDVAEAYEI 720

Query: 721 PDPEFHNFDVDKTSEKFRVGQVWALYSDEDALPKYYAQIKKITHKPEFELRLLWLESSEL 780
           PDPEFHNFDVDKTSEKFRVGQVWALYSDEDALPKYYAQIKKITHKPEFELRLLWLESSEL
Sbjct: 721 PDPEFHNFDVDKTSEKFRVGQVWALYSDEDALPKYYAQIKKITHKPEFELRLLWLESSEL 780

Query: 781 PDDTIEWHDKSMPISCGRFRTQRTKMHSYYSTKSFSHLVRTIPVPKNGFYISPSLGEVWA 840
           PDDTIEWHDKSMPISCGRFRTQRTKMHSYYSTKSFSHLVRTIPVPKNGFYISPSLGEVWA
Sbjct: 781 PDDTIEWHDKSMPISCGRFRTQRTKMHSYYSTKSFSHLVRTIPVPKNGFYISPSLGEVWA 840

Query: 841 LYKNWTPEIRCSDMDKCEYVIVEVIEDDDLQKEVLVLDRVHGFNSVFKAKTKGDSSTVTM 900
           LYKNWTPEIRCSDMDKCEYVIVEVIEDDDLQKEVLVLDRVHGFNSVFKAKTKGDSSTVTM
Sbjct: 841 LYKNWTPEIRCSDMDKCEYVIVEVIEDDDLQKEVLVLDRVHGFNSVFKAKTKGDSSTVTM 900

Query: 901 VITRVELLRFSHRIPAFRLTDERGGSLRGCLELDPAALPIYFFY 945
           VITRVELLRFSHRIPAFRLTDERGGSLRGCLELDPAALPIYFFY
Sbjct: 901 VITRVELLRFSHRIPAFRLTDERGGSLRGCLELDPAALPIYFFY 944

BLAST of MS001728 vs. ExPASy TrEMBL
Match: A0A6J1H7A6 (uncharacterized protein LOC111460259 OS=Cucurbita moschata OX=3662 GN=LOC111460259 PE=4 SV=1)

HSP 1 Score: 1421.4 bits (3678), Expect = 0.0e+00
Identity = 717/952 (75.32%), Postives = 793/952 (83.30%), Query Frame = 0

Query: 1   MDCNKEEAIRARDIAEKKMESKDFTGARKFILRAQQLNPDAENISQMLMVCDVHCAAEKK 60
           MDCNK+EAI+AR+IAEKKMESKDFTGARK +L+AQQLNPDAENISQMLMVCDVHCAAEKK
Sbjct: 1   MDCNKDEAIKAREIAEKKMESKDFTGARKLVLKAQQLNPDAENISQMLMVCDVHCAAEKK 60

Query: 61  LLGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120
           LLGNEMDWY ILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD
Sbjct: 61  LLGNEMDWYAILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120

Query: 121 QEKRRLHDMRCKPAVPYRPPQRAGSTFNVGVQANFRGNFTAFTPQHPQPQPQGHSGFGQN 180
           QEKRRLHDMR +PA+PY+PP RA  +FNVGVQANFR NFT+F PQH  PQ QGHSGF  N
Sbjct: 121 QEKRRLHDMRRRPAIPYQPPHRAAPSFNVGVQANFRSNFTSFIPQHQPPQQQGHSGFSHN 180

Query: 181 RATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNLHHTTFFQ 240
           RATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQG+HPQPMSNL+ T+FFQ
Sbjct: 181 RATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGSHPQPMSNLNQTSFFQ 240

Query: 241 QQNSFNHRAEMGHPGNSQSEKRRMESLRRTHNTSGAASEKLYGKKRRKQTSESSESCDTG 300
           QQNSFNH+AE G PGNSQS+KR     RRT +TSGAASEK  GKK+RKQTSESSES DTG
Sbjct: 241 QQNSFNHKAEAGRPGNSQSDKR-----RRTRSTSGAASEKFTGKKKRKQTSESSESFDTG 300

Query: 301 SSSDFEENVVTNEDSKKDVGRPGAHHPRRSSRQRHKISYNENLSDDDS---VITPKKSKR 360
           +SSD EENVVT+EDS+KDVG  G    RRS R R K+SYNEN+SDDD+   VITPKKSKR
Sbjct: 301 TSSDQEENVVTDEDSRKDVGCSGEQRQRRSVRSRQKVSYNENVSDDDNDDFVITPKKSKR 360

Query: 361 SKSSDSSDEDSEEVYVDEASKINNQSGSADVDDDQRETNKLGHNCSEASLSRRSKGGKKV 420
            KSS  SD+D EEVYVD ASKINN   S D+D D++E NK  ++CSE +LS R+KG KK 
Sbjct: 361 RKSSIVSDDDVEEVYVDGASKINNHYRSVDLDADRKEVNKQENHCSERNLSHRNKGSKKA 420

Query: 421 SHKETLDNDYSQRSMGLAGDPEVNLFSCSDPDFNDFDKLRNRECFKLGQIWAMYDNIDTM 480
           SHKET D D SQ S   AGDP+ N  SCSDPDF+DFDKLRNRECF LGQIWAMYD+IDTM
Sbjct: 421 SHKETADKDVSQGSTESAGDPKCNFLSCSDPDFHDFDKLRNRECFALGQIWAMYDDIDTM 480

Query: 481 PRFYARVKKVFPSGFKVQITWLEPEANDECQSKCVDEKVPVSCGEFVSGATETMTDCGSM 540
           PRFYA +KKVFPSGFKVQITWLEPE  D+ + KCV++++P+SCGEFV GATETMTDCGSM
Sbjct: 481 PRFYAWIKKVFPSGFKVQITWLEPEVTDDGRRKCVNKEMPISCGEFVFGATETMTDCGSM 540

Query: 541 FSHAVSLVKGGRKDIFKIYPRKGQIWALFKNWDKNYNRDSNCQYEYEFVETLSEYSKESG 600
           FSH VS  KG RKD FKIYPRKG+IWALFKNWDKN N DSN +YEYEFVETLSE+++E+G
Sbjct: 541 FSHVVSWDKGCRKDSFKIYPRKGEIWALFKNWDKNSNCDSNSRYEYEFVETLSEFTEEAG 600

Query: 601 IDVALLAKVKGFSCLFCRMVKEGENSFRVPAAELFRFSHQVPSFPLTGDERDDVPEGSFE 660
           IDVA LAKVKGFSCLFCR VKEG    RVPAAELFRFSH++PSFPLTGDER+DVPE S E
Sbjct: 601 IDVAFLAKVKGFSCLFCRTVKEGGKPVRVPAAELFRFSHRIPSFPLTGDEREDVPEDSLE 660

Query: 661 LDPAALPPNVPEIAIPQHLKKVAANAGLNTLKSTAGPNGDAGTHKADADTKSDDGDV--- 720
           LDPAALPPNV EI IP  LK++A  A +                K    ++SDDG+V   
Sbjct: 661 LDPAALPPNVTEIVIPVQLKEMATAADI----------------KTGLRSESDDGNVPVA 720

Query: 721 --AEAYEIPDPEFHNFDVDKTSEKFRVGQVWALYSDEDALPKYYAQIKKITHKPEFELRL 780
              EAYEIPDPEFHNFDVDK+SEKF +GQVWALYSDEDALP+YY  IKKIT +P FE++L
Sbjct: 721 SSGEAYEIPDPEFHNFDVDKSSEKFAIGQVWALYSDEDALPRYYGLIKKITREPRFEVKL 780

Query: 781 LWLESSELPDDTIEWHDKSMPISCGRFRTQ-RTKMHSYYSTKSFSHLVRTIPVPKNGFYI 840
            WLESS LP+DTIEWHDK M I CGRFRTQ RT MH   ST  FSHLVRT   P +GF I
Sbjct: 781 TWLESSSLPNDTIEWHDKGMLIPCGRFRTQRRTTMHCCTSTDPFSHLVRTDSAPNSGFSI 840

Query: 841 SPSLGEVWALYKNWTPEIRCSDMDKCEYVIVEVIEDDDLQKEVLVLDRVHGFNSVFKAKT 900
            P  GEVWALYKNWTPEIR SD++KCEY I EVI+DDD QKEV+VL RV G NS+FKA+T
Sbjct: 841 FPRTGEVWALYKNWTPEIRPSDLEKCEYDIAEVIDDDDSQKEVMVLKRVDGHNSIFKAQT 900

Query: 901 KGDSSTVTMVITRVELLRFSHRIPAFRLTDERGGSLRGCLELDPAALPIYFF 944
           K D ST+TM I + ELLRFSH+IPAFRLTDE GGSLRGCLELDPAALP+Y+F
Sbjct: 901 KNDGSTLTMTIPQAELLRFSHQIPAFRLTDELGGSLRGCLELDPAALPVYYF 931

BLAST of MS001728 vs. ExPASy TrEMBL
Match: A0A5A7SVX4 (DNAJ heat shock N-terminal domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G001180 PE=4 SV=1)

HSP 1 Score: 1417.9 bits (3669), Expect = 0.0e+00
Identity = 721/960 (75.10%), Postives = 802/960 (83.54%), Query Frame = 0

Query: 1   MDCNKEEAIRARDIAEKKMESKDFTGARKFILRAQQLNPDAENISQMLMVCDVHCAAEKK 60
           MDCNK+EAI+AR+IAEKKM  KDF GARK +L+AQQLNPDAE ISQMLMVCDVHCAAEKK
Sbjct: 1   MDCNKDEAIKAREIAEKKMAGKDFIGARKLVLKAQQLNPDAEYISQMLMVCDVHCAAEKK 60

Query: 61  LLGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120
           L GNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD
Sbjct: 61  LFGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120

Query: 121 QEKRRLHDMRCKPAVPYRPPQRAGSTFNVGVQANFRGNFTAFTPQH-PQPQPQGHSGFGQ 180
            EKRR+HDMR KPA+PYRPP RA STFNVGVQAN+R NFT F PQH P PQPQGHSGF  
Sbjct: 121 HEKRRMHDMRRKPAIPYRPPHRAASTFNVGVQANYRSNFTTFIPQHQPPPQPQGHSGFSH 180

Query: 181 NRATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNLHHTTFF 240
           NR+TFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNL+ T+FF
Sbjct: 181 NRSTFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNLNQTSFF 240

Query: 241 QQQNSFNHRAEMGHPGNSQSEKRRMESLRRTHNTSGAASEKLYGKKRRKQTSESSESCDT 300
           QQQNSFNHR EMG PGNSQSEKR+ +      NTS  ASEK+ GKKRRKQTSESSESCDT
Sbjct: 241 QQQNSFNHRPEMGRPGNSQSEKRQGK-----RNTSVTASEKINGKKRRKQTSESSESCDT 300

Query: 301 GSSSDFEENVVTNED-SKKDVGRPGAHHPRRSSRQRHKISYNENLSDDDS---VITPKKS 360
            SS + +EN VT++D S+KDVG  G H PRRSSR+R KISYNEN SDDD+    +T K+S
Sbjct: 301 ESSLETDENGVTDDDSSRKDVGHSGDHRPRRSSRRRQKISYNENGSDDDNDDIEVTHKRS 360

Query: 361 KRSKSSDSSDEDSEEVYVDEASKINNQSGSADVDDDQRETNKLGHNCSEASLSRRSKGGK 420
           KR KSS  SD+D EEV             S  VDDDQ +TNK  ++CSE  LSRRSKG K
Sbjct: 361 KRRKSSIVSDDDIEEV-------------SVAVDDDQTDTNKPENHCSEEDLSRRSKGSK 420

Query: 421 KVSHKETLDNDYSQRSMGLAGDPEVNLFSCSDPDFNDFDKLRNRECFKLGQIWAMYDNID 480
           K +HKET D D SQ SM  AGDPE NL SCSDPDF+DFD+LRNRECF LGQIWAMYD+ID
Sbjct: 421 KENHKETTDQDVSQGSMESAGDPESNLLSCSDPDFHDFDQLRNRECFALGQIWAMYDDID 480

Query: 481 TMPRFYARVKKVFPSGFKVQITWLEPEANDECQSKCVDEKVPVSCGEFVSGATETMTDCG 540
           TMPRFYA +KKVFPSGFKVQITWLEPEA+ + + KCVD+++PVSCGEFV GATETMTDC 
Sbjct: 481 TMPRFYAWIKKVFPSGFKVQITWLEPEASVDGRRKCVDKEMPVSCGEFVFGATETMTDCD 540

Query: 541 SMFSHAVSLVKGGRKDIFKIYPRKGQIWALFKNWDKNYNRDSNCQYEYEFVETLSEYSKE 600
           SMFSHAVS VKG  KD FKIYPRKG+IWALFKNWDKN   DSN QYEYEFVE LSE+++E
Sbjct: 541 SMFSHAVSWVKGCHKDSFKIYPRKGEIWALFKNWDKNRECDSNGQYEYEFVEILSEFTEE 600

Query: 601 SGIDVALLAKVKGFSCLFCRMVKEGENSFRVPAAELFRFSHQVPSFPLTGDERDDVPEGS 660
           +GIDVALLAKVKGFS LFCRMVK GE S++VPAAELFRFSH+VPSFPLTGDER+DVP GS
Sbjct: 601 AGIDVALLAKVKGFSSLFCRMVKVGEKSYQVPAAELFRFSHRVPSFPLTGDEREDVPRGS 660

Query: 661 FELDPAALPPNVPEIAIPQHLKKVAANAGLNTLKSTAGPNGDAGTHKADADTKS------ 720
           FELDPAALPPN+PEI +P+H+K+VA++   +TL    G NGDAGTH+A  DT S      
Sbjct: 661 FELDPAALPPNLPEIILPEHIKEVASDIRGSTL-PMMGSNGDAGTHEAAGDTNSNLRSEG 720

Query: 721 DDGDVA-----EAYEIPDPEFHNFDVDKTSEKFRVGQVWALYSDEDALPKYYAQIKKITH 780
           DDG  A     EAYEIPDPEFHNFD++K+ EKFR+GQVW+LYSDEDALP+YY  IKK+T 
Sbjct: 721 DDGIAAVASPSEAYEIPDPEFHNFDLEKSIEKFRIGQVWSLYSDEDALPRYYGLIKKVTR 780

Query: 781 KPEFELRLLWLESSELPDDTIEWHDKSMPISCGRFRTQ-RTKMHSYYSTKSFSHLVRTIP 840
           +P FE++L WL SS LP DT++WHDK MPISCGRF TQ RT MH Y S  SFSHL+R  P
Sbjct: 781 EP-FEVKLTWLVSSTLPSDTVKWHDKRMPISCGRFTTQRRTAMHRYTSIDSFSHLLRADP 840

Query: 841 VPKNGFYISPSLGEVWALYKNWTPEIRCSDMDKCEYVIVEVIEDDDLQKEVLVLDRVHGF 900
            P N F ISP +GEVWALYKNWTPEIRCSD+DKCEY I EVI+DDDLQKEV+ L RV G+
Sbjct: 841 APNNAFSISPRIGEVWALYKNWTPEIRCSDLDKCEYDIAEVIDDDDLQKEVMFLKRVDGY 900

Query: 901 NSVFKAKTKGDSSTVTMVITRVELLRFSHRIPAFRLTDERGGSLRGCLELDPAALPIYFF 944
           NSVFKA+TK D ST+TM+IT  E+LRFSH+IPAFRLTDERGGSLRGCLELDPAALP+Y+F
Sbjct: 901 NSVFKAQTKNDGSTLTMLITDAEILRFSHQIPAFRLTDERGGSLRGCLELDPAALPVYYF 940

BLAST of MS001728 vs. ExPASy TrEMBL
Match: A0A6J1L1G3 (uncharacterized protein LOC111498998 OS=Cucurbita maxima OX=3661 GN=LOC111498998 PE=4 SV=1)

HSP 1 Score: 1417.1 bits (3667), Expect = 0.0e+00
Identity = 714/952 (75.00%), Postives = 795/952 (83.51%), Query Frame = 0

Query: 1   MDCNKEEAIRARDIAEKKMESKDFTGARKFILRAQQLNPDAENISQMLMVCDVHCAAEKK 60
           MDCNK+EAI+AR+IAEKKMESKDFTGARK +L+AQQLNPDAENISQMLMVCDVHCAAEKK
Sbjct: 1   MDCNKDEAIKAREIAEKKMESKDFTGARKLVLKAQQLNPDAENISQMLMVCDVHCAAEKK 60

Query: 61  LLGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120
           LLGNEMDWY ILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD
Sbjct: 61  LLGNEMDWYAILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120

Query: 121 QEKRRLHDMRCKPAVPYRPPQRAGSTFNVGVQANFRGNFTAFTPQHPQPQPQGHSGFGQN 180
           QEKRRLHDMR +PA+PY+PP RA S+FNVGVQANFR NFT+F PQH  PQ QGHSGF  N
Sbjct: 121 QEKRRLHDMRRRPAIPYQPPHRAASSFNVGVQANFRSNFTSFIPQHQPPQQQGHSGFSHN 180

Query: 181 RATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNLHHTTFFQ 240
           RATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNL+  +FFQ
Sbjct: 181 RATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNLNQASFFQ 240

Query: 241 QQNSFNHRAEMGHPGNSQSEKRRMESLRRTHNTSGAASEKLYGKKRRKQTSESSESCDTG 300
           QQNSFNH+A+ G PGNSQS+KR     RRT +TS AASEK  GKK+RKQTSESSES DTG
Sbjct: 241 QQNSFNHKADTGRPGNSQSDKR-----RRTRSTSAAASEKFTGKKKRKQTSESSESFDTG 300

Query: 301 SSSDFEENVVTNEDSKKDVGRPGAHHPRRSSRQRHKISYNENLSDDDS---VITPKKSKR 360
           +SSD EENVVT+EDS+KDVG  G    RRS R R K+SYNEN+SDDD+   VIT KKSKR
Sbjct: 301 TSSDQEENVVTDEDSRKDVGCSGEQRQRRSVRSRQKVSYNENVSDDDNDDFVITLKKSKR 360

Query: 361 SKSSDSSDEDSEEVYVDEASKINNQSGSADVDDDQRETNKLGHNCSEASLSRRSKGGKKV 420
            KSS  SD+D EEVYVD ASKINN   S D+D D++E NK  ++CSE +LS R+KG KK 
Sbjct: 361 RKSSIVSDDDVEEVYVDGASKINNHYRSVDLDADRKEVNKQENHCSERNLSHRNKGSKKA 420

Query: 421 SHKETLDNDYSQRSMGLAGDPEVNLFSCSDPDFNDFDKLRNRECFKLGQIWAMYDNIDTM 480
           SHKET D D SQ SM  AGD + N  SCSDPDF+DFDKLRNRECF LGQIWAMYD+IDTM
Sbjct: 421 SHKETADKDVSQGSMESAGDTKCNFLSCSDPDFHDFDKLRNRECFALGQIWAMYDDIDTM 480

Query: 481 PRFYARVKKVFPSGFKVQITWLEPEANDECQSKCVDEKVPVSCGEFVSGATETMTDCGSM 540
           PRFYA +KKVFPSGFKVQITWLEPE  D+ + KCV++++P+SCGEFV G TETMTDCGSM
Sbjct: 481 PRFYAWIKKVFPSGFKVQITWLEPEVTDDGRRKCVNKEMPISCGEFVFGETETMTDCGSM 540

Query: 541 FSHAVSLVKGGRKDIFKIYPRKGQIWALFKNWDKNYNRDSNCQYEYEFVETLSEYSKESG 600
           FSHAVS  KG RKD FKIYPRKG+IWALFKNWDKN N DSN +Y+YEFVETLSE+++E+G
Sbjct: 541 FSHAVSWDKGCRKDSFKIYPRKGEIWALFKNWDKNSNCDSNSRYDYEFVETLSEFTEEAG 600

Query: 601 IDVALLAKVKGFSCLFCRMVKEGENSFRVPAAELFRFSHQVPSFPLTGDERDDVPEGSFE 660
           IDVA LAKVKGFSCLFCRMVKEG    RVPAAELFRFSH++PSFPLTGDER+DVPE S E
Sbjct: 601 IDVAFLAKVKGFSCLFCRMVKEGGKPVRVPAAELFRFSHRIPSFPLTGDEREDVPENSLE 660

Query: 661 LDPAALPPNVPEIAIPQHLKKVAANAGLNTLKSTAGPNGDAGTHKADADTKSDDGDV--- 720
           LDPAALPPNVPEI IP  L ++AA A +                K    ++SDDG+V   
Sbjct: 661 LDPAALPPNVPEIVIPVQLNEMAAAADI----------------KTGLRSESDDGNVPVA 720

Query: 721 --AEAYEIPDPEFHNFDVDKTSEKFRVGQVWALYSDEDALPKYYAQIKKITHKPEFELRL 780
              EAYEIPDPEFHNFDV+K+SEKF +GQVWALYSDEDALP+YY  IKKIT +P FE++L
Sbjct: 721 SSGEAYEIPDPEFHNFDVNKSSEKFGIGQVWALYSDEDALPRYYGLIKKITREPRFEVKL 780

Query: 781 LWLESSELPDDTIEWHDKSMPISCGRFRTQ-RTKMHSYYSTKSFSHLVRTIPVPKNGFYI 840
            WLESS LP+DTIEWHDK M I CGRFRTQ RT MH   ST  FSHLVRT   P +GF I
Sbjct: 781 TWLESSALPNDTIEWHDKGMLIPCGRFRTQRRTIMHCCTSTDPFSHLVRTDSAPNSGFSI 840

Query: 841 SPSLGEVWALYKNWTPEIRCSDMDKCEYVIVEVIEDDDLQKEVLVLDRVHGFNSVFKAKT 900
            P  GEVWALYKNWTPEIR SD++KCEY I EVI+DDD QKEV+VL+R+ G NS+FKA+T
Sbjct: 841 FPRTGEVWALYKNWTPEIRSSDLEKCEYDIAEVIDDDDSQKEVMVLNRIDGHNSIFKAQT 900

Query: 901 KGDSSTVTMVITRVELLRFSHRIPAFRLTDERGGSLRGCLELDPAALPIYFF 944
           K D ST+TM+I + ELLRFSH+IPAFRLTDE GGSLRGCLELDPAALP+Y+F
Sbjct: 901 KNDGSTLTMIIPQAELLRFSHQIPAFRLTDELGGSLRGCLELDPAALPVYYF 931

BLAST of MS001728 vs. ExPASy TrEMBL
Match: A0A0A0K9M5 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G067950 PE=4 SV=1)

HSP 1 Score: 1393.3 bits (3605), Expect = 0.0e+00
Identity = 712/960 (74.17%), Postives = 798/960 (83.12%), Query Frame = 0

Query: 1   MDCNKEEAIRARDIAEKKMESKDFTGARKFILRAQQLNPDAENISQMLMVCDVHCAAEKK 60
           MDCNK+EAI+AR+IAEKKME KDFTGARK +L+AQQLNPDAE ISQMLMVCDVHCAAEKK
Sbjct: 1   MDCNKDEAIKAREIAEKKMEGKDFTGARKLVLKAQQLNPDAEYISQMLMVCDVHCAAEKK 60

Query: 61  LLGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120
           L GNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD
Sbjct: 61  LFGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120

Query: 121 QEKRRLHDMRCKPAVPYRPPQRAGSTFNVGVQANFRGNFTAFTPQ-HPQPQPQGHSGFGQ 180
            EKRR+HDMR KPA+P+RPP RA STFNVGVQAN+R NFT F PQ  P PQPQGHSGFG 
Sbjct: 121 HEKRRMHDMRRKPAIPFRPPHRAASTFNVGVQANYRSNFTTFIPQPPPPPQPQGHSGFGH 180

Query: 181 NRATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNLHHTTFF 240
           NR+TFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNL+ T+FF
Sbjct: 181 NRSTFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMSNLNQTSFF 240

Query: 241 QQQNSFNHRAEMGHPGNSQSEKRRMESLRRTHNTSGAASEKLYGKKRRKQTSESSESCDT 300
            QQNSFNHRAEMG PGNSQSEKRR +      NTS AASEK  GKKRRKQTSESSESCDT
Sbjct: 241 PQQNSFNHRAEMGRPGNSQSEKRRGK-----RNTSVAASEKFNGKKRRKQTSESSESCDT 300

Query: 301 GSSSDFEENVVTNED-SKKDVGRPGAHHPRRSSRQRHKISYNENLSDDDS--VITPKKSK 360
            SS D +EN VT++D S+KD+G  G H PRRSSR+R KISYNEN SDDD+   IT K+ +
Sbjct: 301 ESSLDTDENGVTDDDSSRKDIGHSGDHRPRRSSRRRQKISYNENGSDDDNDVEITHKRPR 360

Query: 361 RSKSS-DSSDEDSEEVYVDEASKINNQSGSADVDDDQRETNKLGHNCSEASLSRRSKGGK 420
           R KSS  S DED EEV             S  VDDDQ +TNK  ++CSE  LSRRSKG K
Sbjct: 361 RRKSSIVSDDEDIEEV-------------SVAVDDDQTKTNKPENHCSEEDLSRRSKGSK 420

Query: 421 KVSHKETLDNDYSQRSMGLAGDPEVNLFSCSDPDFNDFDKLRNRECFKLGQIWAMYDNID 480
           + +HKET D D SQ SM  AGDP+ NL SCSDPDF+DFD+LRNRECF LGQIWAMYD+ID
Sbjct: 421 E-NHKETTDQDVSQGSMESAGDPDSNLLSCSDPDFHDFDQLRNRECFTLGQIWAMYDDID 480

Query: 481 TMPRFYARVKKVFPSGFKVQITWLEPEANDECQSKCVDEKVPVSCGEFVSGATETMTDCG 540
           TMPRFYA +KKVFPSGFKVQITWLEPEA+ + + KCVD+++PVSCGEFV GATETMTDC 
Sbjct: 481 TMPRFYAWIKKVFPSGFKVQITWLEPEASVDGRRKCVDKEMPVSCGEFVFGATETMTDCD 540

Query: 541 SMFSHAVSLVKGGRKDIFKIYPRKGQIWALFKNWDKNYNRDSNCQYEYEFVETLSEYSKE 600
           SMFSHAV+  KG  KD F+IYPRKG+IWALFKNWDK    DSN QYEYEFVE LSE+++E
Sbjct: 541 SMFSHAVAWDKGYHKDSFRIYPRKGEIWALFKNWDKKSECDSNGQYEYEFVEILSEFTEE 600

Query: 601 SGIDVALLAKVKGFSCLFCRMVKEGENSFRVPAAELFRFSHQVPSFPLTGDERDDVPEGS 660
           +GIDVALLAKVKGFSCLFCRMVK GE SF+VPAAELFRFSH+VPSFPLTGDER+ VP GS
Sbjct: 601 AGIDVALLAKVKGFSCLFCRMVKVGEKSFQVPAAELFRFSHRVPSFPLTGDEREGVPRGS 660

Query: 661 FELDPAALPPNVPEIAIPQHLKKVAANAGLNTLKSTAGPNGDAGTHKADADTKS------ 720
           FELDPAALPPN+PEI +P+H+K+V ++   +TL    G NG+A TH+A ADT S      
Sbjct: 661 FELDPAALPPNLPEIILPEHIKEVDSDTRRSTL-PMMGSNGEASTHEAAADTNSNLRSEG 720

Query: 721 DDGDVA-----EAYEIPDPEFHNFDVDKTSEKFRVGQVWALYSDEDALPKYYAQIKKITH 780
           DDG  A     EAYEIPDPEFHNFD++K+ EKFR+GQVW+LYSDEDALP+YY  IKK+T 
Sbjct: 721 DDGIAAVASPSEAYEIPDPEFHNFDLEKSIEKFRIGQVWSLYSDEDALPRYYGLIKKVTR 780

Query: 781 KPEFELRLLWLESSELPDDTIEWHDKSMPISCGRFRTQ-RTKMHSYYSTKSFSHLVRTIP 840
           +P FE++L WL SS LP DT++WHDK MPISCGRF  Q RT MH   +  SFSHL+RT P
Sbjct: 781 EP-FEVKLTWLVSSTLPSDTVKWHDKQMPISCGRFTIQRRTPMHRCTTIDSFSHLLRTDP 840

Query: 841 VPKNGFYISPSLGEVWALYKNWTPEIRCSDMDKCEYVIVEVIEDDDLQKEVLVLDRVHGF 900
            P N F ISP +GEVWALYKNWTPE+RCSD+D CEY I EVI+DDDLQKEV+ L RV G+
Sbjct: 841 APNNAFSISPRIGEVWALYKNWTPELRCSDLDNCEYDIAEVIDDDDLQKEVMFLKRVDGY 900

Query: 901 NSVFKAKTKGDSSTVTMVITRVELLRFSHRIPAFRLTDERGGSLRGCLELDPAALPIYFF 944
           NSVFKA+TK D ST+TM+IT  E+LRFSH+IPAFRLT+ERGGSLRGCLELDPAALP+Y+F
Sbjct: 901 NSVFKAQTKNDGSTLTMLITDAEILRFSHQIPAFRLTEERGGSLRGCLELDPAALPVYYF 939

BLAST of MS001728 vs. TAIR 10
Match: AT5G18750.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 606.3 bits (1562), Expect = 4.2e-173
Identity = 380/967 (39.30%), Postives = 545/967 (56.36%), Query Frame = 0

Query: 4   NKEEAIRARDIAEKKMESKDFTGARKFILRAQQLNPDAEN-ISQMLMVCDVHCAAEKKLL 63
           NK+EA+RA+D+AE  M   DFT AR+  ++AQ+++   E+ +++M+MVCDVHCAA +K  
Sbjct: 3   NKDEALRAKDLAEDWMSKSDFTTARRIAIKAQKMDATLESVVARMIMVCDVHCAALEK-S 62

Query: 64  GNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLDQE 123
           G+E DWY ILQ+EQTA+E TI+KQY+K AL LHPDKNK  GAE+AFK +GEAQRVLLD++
Sbjct: 63  GDETDWYKILQVEQTADENTIKKQYKKLALHLHPDKNKLPGAESAFKTIGEAQRVLLDKD 122

Query: 124 KRRLHDMRCK-----------PAVPYRPPQRAGST---FNVGVQANFRGNFTAFTPQH-- 183
           KRR HDMR K           PA  ++PPQ+A +T      G Q N   N     P++  
Sbjct: 123 KRRFHDMRRKPVFRRPAPAPAPAPSFQPPQQAPTTPFFTQRGFQTNV--NVARKRPENQK 182

Query: 184 -PQPQPQGHSGFGQNRATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGA 243
            PQ QP G  G     A+F T C FC  +Y+Y ++++N  + C NC K +VA+    Q  
Sbjct: 183 KPQAQPTGFDGL----ASFTTSCAFCHRKYEYQRKLINTLMTCLNCGKQYVAFQETFQPP 242

Query: 244 HPQPMSNLHHTTFFQQQNSFNHRAEMGHPGNSQSEKRRMESLRRTH-NTSGAASEKLYGK 303
                S      FFQQ       A        +S  +   S   +   +SG ++E + GK
Sbjct: 243 VQPTFS------FFQQSKVPTQEAGKAVEKQPESCAKSFFSKEGSRAKSSGVSAENINGK 302

Query: 304 KRRKQTSESSESCDTGSSSDFEENVVTNEDSKKDVGRPGAHHPRRSSRQRHKISYNENLS 363
           ++RK+  ESS+S  + SS D  E     +DS    G  GA H RRS R + ++SY E+  
Sbjct: 303 RKRKKVVESSDSSCSESSIDCNEVPAGGQDS----GSSGAQHSRRSVRSKQQVSYKEDKE 362

Query: 364 DDDSVITPKKSKRSKSSDSSDEDSEEVYVDEASKINNQSGSADVDDDQRETNKLGHNCSE 423
           +D           ++S++ SD   +                    +DQ  T  L +  + 
Sbjct: 363 ED-----------AESAEESDFRKKS------------------HEDQPFTETLPNGLNR 422

Query: 424 ASLSRRSKGGKKVSHKETLDNDYSQRSMGLAGDPEVNLFSCSDPDFNDFDKLRNRECFKL 483
              S+ +K                  S G A D E+    C+DPDF++F+K R   CFK 
Sbjct: 423 TKKSKAAKNS----------------SSGSASDAEI---QCTDPDFSNFEKSREVTCFKA 482

Query: 484 GQIWAMYDNIDTMPRFYARVKKVF-PSGFKVQITWLEPEANDECQSKCVDEKVPVSCGEF 543
           GQ WA+YD++  MPR+YA ++KV     F ++I WLE E +DE  +  V + +P+S G+F
Sbjct: 483 GQTWAIYDDMGGMPRYYAIIRKVIRKPSFMLKIQWLEAEPDDEKANLWVRKNLPISIGKF 542

Query: 544 VSGATETM--TDCGSMFSHAVSLVKGGRKDIFKIYPRKGQIWALFKNWDKNYN----RDS 603
             G  E +  T C   FSH +    G  KD  ++YPR G+ WALFKNWD N++    R S
Sbjct: 543 KLGGNENIEKTPC---FSHMIYFKVGSMKDTVRVYPRIGETWALFKNWDINWSSGRRRSS 602

Query: 604 N-CQYEYEFVETLSEYSKESGIDVALLAKVKGFSCLFCRMVK-EGENSFRVPAAELFRFS 663
           +  +YEYEFVE LSEY +   I VA L K+KGF+ +FCR+    G ++ ++P  EL RFS
Sbjct: 603 HEHEYEYEFVEILSEYVEGVAIQVAFLRKIKGFTSVFCRIAPGGGSDTIQIPPHELLRFS 662

Query: 664 HQVPSFPLTGDERDDVPEGSFELDPAALPPNV-PEIAIPQHLKKVAANAGLNTLKSTAGP 723
           H +PS  LTG E + VP GS+E D AALP  +  E A+P     +  +A LN +   + P
Sbjct: 663 HSIPSTKLTGKEGNGVPIGSYEFDTAALPQKIEQEEAVP----VLRESAKLNQVNHNSPP 722

Query: 724 NGDAGTHKADADTKSDDGDVAEAYEIPDPEFHNFDVDKTSEKFRVGQVWALYSDEDALPK 783
           + +                  +   IP+ +F+NF  ++   KF  GQ+W+L S ED LPK
Sbjct: 723 SSE-----------------PDCIVIPNFQFNNFSAERLEGKFAPGQIWSLNSKEDGLPK 782

Query: 784 YYAQIKKITHKPEFELRLLWLESSELPDDTIEWHDKSMPISCGRFRTQRTKMHSYYSTKS 843
            YA+I++I  +P F+L++  LE   L ++ I+WHDK MP+SCG F  +  +  +      
Sbjct: 783 CYAKIQQIVWRPVFKLQINRLEPKSLLENVIQWHDKRMPVSCGNFTLKEGRDETLTKVTD 842

Query: 844 FSHLVRTIPVPK-NGFYISPSLGEVWALYKNWTPEIRCSDMDKCEYVIVEVIEDDDLQKE 903
           FSH ++     + N + + P  GE+WA+YKNW+  I+ + + +CEY +VEV++D+D   E
Sbjct: 843 FSHQIKAEKHFRINEYIVVPKTGEIWAMYKNWSETIKATSLKRCEYEVVEVLDDNDSHIE 879

Query: 904 VLVLDRVHGFNSVFKAKTKGDSSTVTMVITRVELLRFSHRIPAFRLTDERGGSLRGCLEL 941
           V++L++V GF SVFK K +G    V   I R ELLRFSH +PAFRLT ER G+LRG +EL
Sbjct: 903 VMLLEQVDGFISVFKEKLEG-GIDVKKKIPRCELLRFSHYVPAFRLTGERDGALRGYVEL 879

BLAST of MS001728 vs. TAIR 10
Match: AT3G06340.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 481.1 bits (1237), Expect = 2.0e-135
Identity = 289/684 (42.25%), Postives = 400/684 (58.48%), Query Frame = 0

Query: 1   MDCNKEEAIRARDIAEKKMESKDFTGARKFILRAQQLNPDAENISQMLMVCDVHCAAEKK 60
           M  N++EA+RA+D+AE  M+  DFT ARK  ++AQ+++   ENIS+M+MVCDVHCAA +K
Sbjct: 1   MSINRDEALRAKDLAEGLMKKTDFTAARKLAMKAQKMDSSLENISRMIMVCDVHCAATEK 60

Query: 61  LLGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120
           L G EMDWYGILQ+EQ AN+  I+KQY++ ALLLHPDKNK  GAE+AFKL+GEAQR+LLD
Sbjct: 61  LFGTEMDWYGILQVEQIANDVIIKKQYKRLALLLHPDKNKLPGAESAFKLIGEAQRILLD 120

Query: 121 QEKRRLHDMRCKP-AVPYRPPQRAGSTFNVGVQANFRGN------FTAFTPQ--HPQPQP 180
           +EKR LHD + K    P  PP +A    N   Q +FR +      FT   P+  HP  + 
Sbjct: 121 REKRTLHDNKRKTWRKPAAPPYKAQQMPNYHTQPHFRASVNTRNIFTELRPEIRHPFQKA 180

Query: 181 QGHSGFGQNRATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMS 240
           Q       +  TF T C FC VRY+Y +  VN+ + C+ CKK F A++  LQ A PQ   
Sbjct: 181 QAQPAAFTHLKTFGTSCVFCRVRYEYDRAHVNKEVTCETCKKRFTAFEEPLQSA-PQAKG 240

Query: 241 NLHHTTFFQQQNSFNHRAEMGHPGNSQSEKRRMESLRRTHNTSGAASEKLYGKKRRKQTS 300
               T  F QQ+ F  +     P         + S + +    G+ + K  GK++RK  +
Sbjct: 241 PSQTTYCFPQQSKFPDQRACSEPHKRPENPPTVSSSKASFPMPGSTA-KHNGKRKRKNVA 300

Query: 301 ESSESCDTGSSSDFEENVVTNEDSKKDVGRPGAHHPRRSSRQRHKISYNENLSDDDSVIT 360
           E SES D+ SSS+ E++V  +  + +D G  G   PRRS R + K+SYNENLSDDD  + 
Sbjct: 301 ECSESSDSESSSESEDDVNNDTTAAQDSGSNGGEQPRRSVRSKQKVSYNENLSDDDVDLV 360

Query: 361 PKKSKRSKSSDSSDEDSEEVYVDEASKINNQSGSAD----VDDDQRETNKLGHNCSEASL 420
               + S  +  ++ + E     + ++ ++ + S D    ++ DQ ET   G + SE  L
Sbjct: 361 NDNGEGSGKNIDTEREKETEEEKQTNENHSSTESIDMNGKIEVDQVETPS-GASDSEEDL 420

Query: 421 SRRSKGGKKVSHKETLDNDYSQRSMGLAGDPEVNLFSCSDPDFNDFDKLRNRECFKLGQI 480
                                  S G A  P  NL +  DPDFNDFDKLR + CF+ GQI
Sbjct: 421 -----------------------SSGSAEKP--NLINYDDPDFNDFDKLREKSCFQAGQI 480

Query: 481 WAMYDNIDTMPRFYARVKKVFPSGFKVQITWLEPEANDECQSKCVDEKVPVSCGEFVSGA 540
           WA+YD  + MPRFYA +KKV    F ++  W E + + E ++      +PVS G+FV G 
Sbjct: 481 WAVYDEEEGMPRFYALIKKVTTPDFMLRYVWFEVDQDQENET----PNLPVSVGKFVVGN 540

Query: 541 TETMTDCGSMFSHAVSLVKGGRKDIFKIYPRKGQIWALFKNWDKNYNRD--SNCQYEYEF 600
            E    C S+FSH V      R   F ++P+KG+IWALFKNWD N + D  S  +YEYEF
Sbjct: 541 IEETNLC-SIFSHFVYSTTKIRTRKFTVFPKKGEIWALFKNWDINCSADSVSPMKYEYEF 600

Query: 601 VETLSEYSKESGIDVALLAKVKGFSCLFCRMVKEGENSFRVPAAELFRFSHQVPSFPLTG 660
           VE LS++++ + + V  L+KV+GF+C+FC M K+  N+  +P  E  RFSH +PSF LTG
Sbjct: 601 VEILSDHAEGATVSVGFLSKVQGFNCVFCPMPKDESNTCEIPPHEFCRFSHSIPSFRLTG 651

Query: 661 DERDDVPEGSFELDPAALPPNVPE 670
            E   + +G +ELDPAALP +V +
Sbjct: 661 TEGRGITKGWYELDPAALPASVSQ 651

BLAST of MS001728 vs. TAIR 10
Match: AT3G06340.2 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 481.1 bits (1237), Expect = 2.0e-135
Identity = 289/684 (42.25%), Postives = 400/684 (58.48%), Query Frame = 0

Query: 1   MDCNKEEAIRARDIAEKKMESKDFTGARKFILRAQQLNPDAENISQMLMVCDVHCAAEKK 60
           M  N++EA+RA+D+AE  M+  DFT ARK  ++AQ+++   ENIS+M+MVCDVHCAA +K
Sbjct: 1   MSINRDEALRAKDLAEGLMKKTDFTAARKLAMKAQKMDSSLENISRMIMVCDVHCAATEK 60

Query: 61  LLGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120
           L G EMDWYGILQ+EQ AN+  I+KQY++ ALLLHPDKNK  GAE+AFKL+GEAQR+LLD
Sbjct: 61  LFGTEMDWYGILQVEQIANDVIIKKQYKRLALLLHPDKNKLPGAESAFKLIGEAQRILLD 120

Query: 121 QEKRRLHDMRCKP-AVPYRPPQRAGSTFNVGVQANFRGN------FTAFTPQ--HPQPQP 180
           +EKR LHD + K    P  PP +A    N   Q +FR +      FT   P+  HP  + 
Sbjct: 121 REKRTLHDNKRKTWRKPAAPPYKAQQMPNYHTQPHFRASVNTRNIFTELRPEIRHPFQKA 180

Query: 181 QGHSGFGQNRATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMS 240
           Q       +  TF T C FC VRY+Y +  VN+ + C+ CKK F A++  LQ A PQ   
Sbjct: 181 QAQPAAFTHLKTFGTSCVFCRVRYEYDRAHVNKEVTCETCKKRFTAFEEPLQSA-PQAKG 240

Query: 241 NLHHTTFFQQQNSFNHRAEMGHPGNSQSEKRRMESLRRTHNTSGAASEKLYGKKRRKQTS 300
               T  F QQ+ F  +     P         + S + +    G+ + K  GK++RK  +
Sbjct: 241 PSQTTYCFPQQSKFPDQRACSEPHKRPENPPTVSSSKASFPMPGSTA-KHNGKRKRKNVA 300

Query: 301 ESSESCDTGSSSDFEENVVTNEDSKKDVGRPGAHHPRRSSRQRHKISYNENLSDDDSVIT 360
           E SES D+ SSS+ E++V  +  + +D G  G   PRRS R + K+SYNENLSDDD  + 
Sbjct: 301 ECSESSDSESSSESEDDVNNDTTAAQDSGSNGGEQPRRSVRSKQKVSYNENLSDDDVDLV 360

Query: 361 PKKSKRSKSSDSSDEDSEEVYVDEASKINNQSGSAD----VDDDQRETNKLGHNCSEASL 420
               + S  +  ++ + E     + ++ ++ + S D    ++ DQ ET   G + SE  L
Sbjct: 361 NDNGEGSGKNIDTEREKETEEEKQTNENHSSTESIDMNGKIEVDQVETPS-GASDSEEDL 420

Query: 421 SRRSKGGKKVSHKETLDNDYSQRSMGLAGDPEVNLFSCSDPDFNDFDKLRNRECFKLGQI 480
                                  S G A  P  NL +  DPDFNDFDKLR + CF+ GQI
Sbjct: 421 -----------------------SSGSAEKP--NLINYDDPDFNDFDKLREKSCFQAGQI 480

Query: 481 WAMYDNIDTMPRFYARVKKVFPSGFKVQITWLEPEANDECQSKCVDEKVPVSCGEFVSGA 540
           WA+YD  + MPRFYA +KKV    F ++  W E + + E ++      +PVS G+FV G 
Sbjct: 481 WAVYDEEEGMPRFYALIKKVTTPDFMLRYVWFEVDQDQENET----PNLPVSVGKFVVGN 540

Query: 541 TETMTDCGSMFSHAVSLVKGGRKDIFKIYPRKGQIWALFKNWDKNYNRD--SNCQYEYEF 600
            E    C S+FSH V      R   F ++P+KG+IWALFKNWD N + D  S  +YEYEF
Sbjct: 541 IEETNLC-SIFSHFVYSTTKIRTRKFTVFPKKGEIWALFKNWDINCSADSVSPMKYEYEF 600

Query: 601 VETLSEYSKESGIDVALLAKVKGFSCLFCRMVKEGENSFRVPAAELFRFSHQVPSFPLTG 660
           VE LS++++ + + V  L+KV+GF+C+FC M K+  N+  +P  E  RFSH +PSF LTG
Sbjct: 601 VEILSDHAEGATVSVGFLSKVQGFNCVFCPMPKDESNTCEIPPHEFCRFSHSIPSFRLTG 651

Query: 661 DERDDVPEGSFELDPAALPPNVPE 670
            E   + +G +ELDPAALP +V +
Sbjct: 661 TEGRGITKGWYELDPAALPASVSQ 651

BLAST of MS001728 vs. TAIR 10
Match: AT3G06340.3 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 481.1 bits (1237), Expect = 2.0e-135
Identity = 289/684 (42.25%), Postives = 400/684 (58.48%), Query Frame = 0

Query: 1   MDCNKEEAIRARDIAEKKMESKDFTGARKFILRAQQLNPDAENISQMLMVCDVHCAAEKK 60
           M  N++EA+RA+D+AE  M+  DFT ARK  ++AQ+++   ENIS+M+MVCDVHCAA +K
Sbjct: 1   MSINRDEALRAKDLAEGLMKKTDFTAARKLAMKAQKMDSSLENISRMIMVCDVHCAATEK 60

Query: 61  LLGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120
           L G EMDWYGILQ+EQ AN+  I+KQY++ ALLLHPDKNK  GAE+AFKL+GEAQR+LLD
Sbjct: 61  LFGTEMDWYGILQVEQIANDVIIKKQYKRLALLLHPDKNKLPGAESAFKLIGEAQRILLD 120

Query: 121 QEKRRLHDMRCKP-AVPYRPPQRAGSTFNVGVQANFRGN------FTAFTPQ--HPQPQP 180
           +EKR LHD + K    P  PP +A    N   Q +FR +      FT   P+  HP  + 
Sbjct: 121 REKRTLHDNKRKTWRKPAAPPYKAQQMPNYHTQPHFRASVNTRNIFTELRPEIRHPFQKA 180

Query: 181 QGHSGFGQNRATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYDMELQGAHPQPMS 240
           Q       +  TF T C FC VRY+Y +  VN+ + C+ CKK F A++  LQ A PQ   
Sbjct: 181 QAQPAAFTHLKTFGTSCVFCRVRYEYDRAHVNKEVTCETCKKRFTAFEEPLQSA-PQAKG 240

Query: 241 NLHHTTFFQQQNSFNHRAEMGHPGNSQSEKRRMESLRRTHNTSGAASEKLYGKKRRKQTS 300
               T  F QQ+ F  +     P         + S + +    G+ + K  GK++RK  +
Sbjct: 241 PSQTTYCFPQQSKFPDQRACSEPHKRPENPPTVSSSKASFPMPGSTA-KHNGKRKRKNVA 300

Query: 301 ESSESCDTGSSSDFEENVVTNEDSKKDVGRPGAHHPRRSSRQRHKISYNENLSDDDSVIT 360
           E SES D+ SSS+ E++V  +  + +D G  G   PRRS R + K+SYNENLSDDD  + 
Sbjct: 301 ECSESSDSESSSESEDDVNNDTTAAQDSGSNGGEQPRRSVRSKQKVSYNENLSDDDVDLV 360

Query: 361 PKKSKRSKSSDSSDEDSEEVYVDEASKINNQSGSAD----VDDDQRETNKLGHNCSEASL 420
               + S  +  ++ + E     + ++ ++ + S D    ++ DQ ET   G + SE  L
Sbjct: 361 NDNGEGSGKNIDTEREKETEEEKQTNENHSSTESIDMNGKIEVDQVETPS-GASDSEEDL 420

Query: 421 SRRSKGGKKVSHKETLDNDYSQRSMGLAGDPEVNLFSCSDPDFNDFDKLRNRECFKLGQI 480
                                  S G A  P  NL +  DPDFNDFDKLR + CF+ GQI
Sbjct: 421 -----------------------SSGSAEKP--NLINYDDPDFNDFDKLREKSCFQAGQI 480

Query: 481 WAMYDNIDTMPRFYARVKKVFPSGFKVQITWLEPEANDECQSKCVDEKVPVSCGEFVSGA 540
           WA+YD  + MPRFYA +KKV    F ++  W E + + E ++      +PVS G+FV G 
Sbjct: 481 WAVYDEEEGMPRFYALIKKVTTPDFMLRYVWFEVDQDQENET----PNLPVSVGKFVVGN 540

Query: 541 TETMTDCGSMFSHAVSLVKGGRKDIFKIYPRKGQIWALFKNWDKNYNRD--SNCQYEYEF 600
            E    C S+FSH V      R   F ++P+KG+IWALFKNWD N + D  S  +YEYEF
Sbjct: 541 IEETNLC-SIFSHFVYSTTKIRTRKFTVFPKKGEIWALFKNWDINCSADSVSPMKYEYEF 600

Query: 601 VETLSEYSKESGIDVALLAKVKGFSCLFCRMVKEGENSFRVPAAELFRFSHQVPSFPLTG 660
           VE LS++++ + + V  L+KV+GF+C+FC M K+  N+  +P  E  RFSH +PSF LTG
Sbjct: 601 VEILSDHAEGATVSVGFLSKVQGFNCVFCPMPKDESNTCEIPPHEFCRFSHSIPSFRLTG 651

Query: 661 DERDDVPEGSFELDPAALPPNVPE 670
            E   + +G +ELDPAALP +V +
Sbjct: 661 TEGRGITKGWYELDPAALPASVSQ 651

BLAST of MS001728 vs. TAIR 10
Match: AT5G27240.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 386.3 bits (991), Expect = 6.8e-107
Identity = 337/1138 (29.61%), Postives = 505/1138 (44.38%), Query Frame = 0

Query: 1    MDCNKEEAIRARDIAEKKMESKDFTGARKFILRAQQLNPDAENISQMLMVCDVHCAAEKK 60
            MD NKEEA RA+ +AE KM+  DF GA+K +L+AQ L    E++ QML VCDVH +AEKK
Sbjct: 1    MDWNKEEACRAKTLAEDKMKEGDFVGAQKLLLKAQSLFSGLESLPQMLAVCDVHNSAEKK 60

Query: 61   LLGNEMDWYGILQIEQTANEATIRKQYRKYALLLHPDKNKFIGAEAAFKLVGEAQRVLLD 120
            +   E +WYGILQ+   A++ATI+KQ RK ALLLHPDKN+F GAEAAFKLV +A R L D
Sbjct: 61   INCLE-NWYGILQVMHFADDATIKKQVRKLALLLHPDKNQFPGAEAAFKLVWDASRFLAD 120

Query: 121  QEKRRLHDMRCKPAVPYRPPQRAGSTFNVGVQANFRGNFTAFTPQHPQPQPQGHSGFGQN 180
            ++KR  +D+R +  +     Q      N G+Q     + T                    
Sbjct: 121  KDKRSQYDIRRRIYLRLATNQ---LNANSGLQCAATNSAT-------------------- 180

Query: 181  RATFWTVCPFCSVRYQYYKEVVNRSLCCQNCKKPFVAYD--------------MELQGAH 240
              TFWT C  C  RY+Y ++ VN  L C  C++ ++AYD               E+Q   
Sbjct: 181  -DTFWTCCEHCGYRYKYLRKYVNILLNCNICQRSYMAYDTGFNEAPSKSNTGQKEVQNQG 240

Query: 241  PQPMS-NLHHTTFFQQQNS--------------FNHRAEMGHPGNSQ------------- 300
            P   S N +  +   Q  S              FN R   G    ++             
Sbjct: 241  PCNTSLNTNGESIGAQPGSVAAEVDKKGTFNKKFNKRNGGGESKKTEVKNSKIETEFVRN 300

Query: 301  SEKRRMESLRRTHNTSGAASEKLYGKKRRKQTSESSESCDTGSSS--------DFEENV- 360
             ++ +M+S  + H      +E   G  +       S S +  S+S          EE++ 
Sbjct: 301  DDEEKMKSAAKLHKPHPEVTEPEIGASKSVPDESVSRSDEAPSTSKDKNKMKKGVEESIN 360

Query: 361  VTNEDSKKDVGRPGAHHPRRSSRQRHKISY-NENLSDDDSVITPKKSKRSKSSDSSDEDS 420
            V   D  KD      ++ R+S R+  + SY  E    D+S+  PKK  RS     S++ +
Sbjct: 361  VDGSDFIKDKAYSKDNNKRKSPRRSQQSSYAEEEKISDNSLGPPKKRLRSNVGLKSEQTT 420

Query: 421  EE--VYVDEASKINNQSGSAD------------VDDDQRETNKLGHNCSEASLSRRSKGG 480
             +  V V  + + ++  GS+             VD   +E + +     E     R   G
Sbjct: 421  RKGLVGVGSSKRFDSGGGSSAPSCAFKRKAKKFVDSGYQEISSVKDKEREV----RKASG 480

Query: 481  KKVSHKETLDNDYSQRSMGLAGDPEVNLFS--CSDPDFNDFDKLRNRECFKLGQIWAMYD 540
            + V  +  +DN++         +P  NL +    DP+F++F+      CF + Q+W+MYD
Sbjct: 481  EGVVMEAKIDNNH---------NPNENLITEDLPDPEFSNFE--LTTSCFGVNQVWSMYD 540

Query: 541  NIDTMPRFYARVKKVFPSGFKVQITWLEPEANDECQSKCVDEKVPVSCGEFVSGATETMT 600
             ID MPR YAR+ KV    FK+ ITW++P  +++      D  +P++CG F  G +E   
Sbjct: 541  PIDGMPRLYARIDKVLVPEFKLWITWIDPLQDNK------DNSIPIACGIFQGGGSEEEN 600

Query: 601  DCGSMFSHAVSLVKGGRKDIFKIYPRKGQIWALFKNWDKNYNRDSNCQ---YEYEFVETL 660
            D       +  +    R +   IYPRKG+IWA+F+ WD +++  S      YEY+FVE L
Sbjct: 601  D---HLKFSCQMFHLTRNNSVVIYPRKGEIWAIFRGWDISWSASSENHKHPYEYDFVEVL 660

Query: 661  SEYSKESGIDVALLAKVKGFSCLFCRMVKEGENSFRVPAAELFRFSHQVPSFPLTGDERD 720
            S ++ E+G+ V  L KV+GF  LF +  ++G    ++P +++ RFSH+VPSF +TG ER+
Sbjct: 661  SNFNDENGLGVGFLGKVEGFVSLFRQDAQDGVLQLQIPPSQMLRFSHKVPSFKMTGKERE 720

Query: 721  DVPEGSFELDPAALPPNVPEI--------------------------------------- 780
             VP G FELDPAALP  + E+                                       
Sbjct: 721  GVPPGCFELDPAALPKELFEVYDSKVDVGLDRELPNGKTGGPFPEASKVEMQANTTRESS 780

Query: 781  ------------------------------AIPQHLKKVAANAGLNTLKS---------- 840
                                          +  +  K V A   LN  KS          
Sbjct: 781  PFKKKRPKVHDNHGSFSKESDGRRTNHERNSAKESRKSVKAVDALNLRKSPRLLSETNSQ 840

Query: 841  ------------TAGPNGDA---------------------------GTHKADADTKS-- 900
                         +  NG++                             HK   + K   
Sbjct: 841  ANSRSGRQIAEKKSANNGESCGKPGGLSLSGEKMSEKTITRMRKTPQDIHKPTVNVKKHG 900

Query: 901  -DDGDVAEA------------YEIPDPEFH-------------NFDVDKTSEKFRVGQVW 919
             +D  ++++             +  +PE               NF+  ++ +KF++ Q+W
Sbjct: 901  RNDESLSQSRGDGLLTQLNGNMKSSEPETRVPSSCKTVKENTFNFENQRSWDKFQIDQIW 960

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138503.10.0e+0099.79uncharacterized protein LOC111009660 [Momordica charantia] >XP_022138504.1 uncha... [more]
XP_038907114.10.0e+0076.33uncharacterized protein LOC120092937 [Benincasa hispida] >XP_038907115.1 unchara... [more]
XP_022959199.10.0e+0075.32uncharacterized protein LOC111460259 [Cucurbita moschata][more]
XP_023548310.10.0e+0075.29uncharacterized protein LOC111806985 [Cucurbita pepo subsp. pepo][more]
KAA0033457.10.0e+0075.10DNAJ heat shock N-terminal domain-containing protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q9FH281.2e-1034.78Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2[more]
A0A0P0VG314.4e-1039.66DnaJ protein P58IPK homolog A OS=Oryza sativa subsp. japonica OX=39947 GN=P58A P... [more]
Q5JNB51.3e-0938.79DnaJ protein P58IPK homolog B OS=Oryza sativa subsp. japonica OX=39947 GN=P58B P... [more]
Q7NBW01.7e-0944.78Chaperone protein DnaJ OS=Mycoplasma gallisepticum (strain R(low / passage 15 / ... [more]
Q9QYI48.4e-0930.23DnaJ homolog subfamily B member 12 OS=Mus musculus OX=10090 GN=Dnajb12 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1C9X20.0e+0099.79uncharacterized protein LOC111009660 OS=Momordica charantia OX=3673 GN=LOC111009... [more]
A0A6J1H7A60.0e+0075.32uncharacterized protein LOC111460259 OS=Cucurbita moschata OX=3662 GN=LOC1114602... [more]
A0A5A7SVX40.0e+0075.10DNAJ heat shock N-terminal domain-containing protein OS=Cucumis melo var. makuwa... [more]
A0A6J1L1G30.0e+0075.00uncharacterized protein LOC111498998 OS=Cucurbita maxima OX=3661 GN=LOC111498998... [more]
A0A0A0K9M50.0e+0074.17J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G067950 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G18750.14.2e-17339.30DNAJ heat shock N-terminal domain-containing protein [more]
AT3G06340.12.0e-13542.25DNAJ heat shock N-terminal domain-containing protein [more]
AT3G06340.22.0e-13542.25DNAJ heat shock N-terminal domain-containing protein [more]
AT3G06340.32.0e-13542.25DNAJ heat shock N-terminal domain-containing protein [more]
AT5G27240.16.8e-10729.61DNAJ heat shock N-terminal domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 103..123
score: 44.33
coord: 87..102
score: 50.03
coord: 69..87
score: 34.69
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 66..123
e-value: 1.6E-19
score: 80.9
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 67..128
e-value: 1.4E-17
score: 63.5
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 67..131
score: 14.910659
IPR001623DnaJ domainCDDcd06257DnaJcoord: 67..120
e-value: 5.18894E-16
score: 71.0373
IPR024593Domain of unknown function DUF3444PFAMPF11926DUF3444coord: 717..922
e-value: 8.1E-65
score: 218.4
coord: 442..647
e-value: 9.9E-71
score: 237.7
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 64..174
e-value: 1.2E-18
score: 69.6
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 64..160
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 247..423
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 334..366
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 309..325
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 294..308
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 254..269
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 689..710
NoneNo IPR availablePANTHERPTHR45089:SF36DNAJ HEAT SHOCK AMINO-TERMINAL DOMAIN PROTEINcoord: 1..943
NoneNo IPR availablePANTHERPTHR45089FAMILY NOT NAMEDcoord: 1..943

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS001728.1MS001728.1mRNA