MS001682 (gene) Bitter gourd (TR) v1

Overview


Name	MS001682
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	E3 ubiquitin-protein ligase RHF1A-like isoform X1
Location	scaffold571: 192936 .. 195356 (+)
RNA-Seq Expression	MS001682
Synteny	MS001682

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TGCTGCATCTGCCTCGACCCCTTCACCTCCCACAACCCTCCCACTGTAAGCTTTTTCACTTCTTCCAGATCATACTGACACTGAAACTAGGAGAAGATTTGTTTTACCTATTTAGTTTGGTTCTGCAGTCTTTTAGCTTACTACCAATTGTATTTTGTGATCTTCATATGAGATAATCAGTGAAGCATTTCTTGCTAGCTACTGGCTTGATGTTTAGCTACATGCTCTTTTACTCGTGGTTATTGTTGGTATGAACCGGTGAAGTGATACTGTAGAATGTATGGCTTCTTTGGTTGGGATGTATTGTATACGTTTCAAGTGAGTATATATTCATCAGAATTGTGATATTTAAATTTGCTGCAGAAGTTTCAAGTAATTGAGATCGACCCTTGTCCGGAAGTGAATAATCATATTACTGTTTTAGGTAGTTGAGATCGGATTGGATCATTTCTTCATTCCCTATTGCTAAGGGATTGTTGTCTATTGTTCCAATTTCAGGTTACCTGTTGCAAGCATGAATATCATCTCCAATGTATTCTTGATTGGTAGGCTTGTTGAAGATTTTGTTTTATCTGATCTTTTAAAAAACAAGTTGGTGTAAGTCTTACTTTTTGGGTATTGATTCTTTCAGGTCTCAAAGAAGCAATGAATGCCCAATATGTTGCCAGTTGCTAGTGTTGAAGGATCCTGTTGGGTGTGTAATTTTGTTGTAGAAACTTAATTTCCCCTAATTCTCATGCCTTGTCCTATCTCTCTTAGGGCACTGTTGAGAGTGACTTTAGCAAGAGTGATTTAATTAGAAGTTTGTCAAAAGTTTTTTATTTTATTTTAATGATTGGTTCCACACTTTAAAAGTAATTTTGAATCGTTCATGTTTGGTTTACTCAGTGATTTTACGATCATTAAACTACTGAAAAACACCTCCCGAAAAATAGTTATTTACTTGATAAAAAATCAGTGATGGCCAAAATCACTCCATGCCCTCAGTCTATCTAAGGGATTTAAGCATGTGCTACATTTGTTTTTTCTTGGCCTCTCTCTCTTGCTGAACTGCTATTACAATTCTACAGCCAGGAGCTTATAGTGGCCATGGCTGGTGAAAGGATTTTAAAGTCAAGAAGCATGTCTTCTGCTGCATCCACTTCTCTGCGATTCCATGAAAACTTTGATGTCGACCACGTGAGTAGTTCTAATGAATCTGAAAATTTATGGGACCTCTTTATGCTTAATCTTTGCTTCAGTGAAGGGTCAACCTTTTATAAAGCCAGCAAAATTGACTCCTTCAGGACTCCTCTCACTCGGATGACTCTGACTTTGATGACCTGATTATGCAGCATCTTGCTGCTGCAGCTAGCAGAGCTCGGTATGTTCATAGAAGCGAGAGGCAAAGGCATTTCGTATCTCCAGAAGGGGTTCCCCAGCAAACATGCCCATTGTCGCCTCGATATCAGGATCCAACTCTAAGTTCACCTATTAGTGATTCGCCATCTCCTGGTTCCATATCTGAGTTTCATGTTCAAGCTTTGTCATCTGTGGGCCTCCATGATGGCAATGAGTTCTCAAGAACTGGAACTAATAAAATATCGCCCAGGTACATCCCCGGACTTGTATATCGACTGTATGCTTTTGCTCCTCAACACCAACAATAAATTAAATTAACCATTGAGTTGCTTTCTTCTGTGGGATGCAAAAAGTTAGCAGTAGAAGAGAGAGTGCCTTGCTAGTAGTCTATCAAGAATAAAGTCTTTCTTCCATTGATTGCAACCTTAGATTTTCAAGTTGCCCTCAAGCTTGAAAAACAAGTAATGATTTCGTTTGTTTTACTGTCTAACATAGATCACACTTCTGTGTCATATAGGGCTTTACTGACTGAGACGTCATCTGGTAGTCAACAGAAAACAAACCAATCTGAGGGGCTCTCTTTCCCTGATTCCATCAAATCCAAAATTTCTTCTACTTCCACAAAGTCAGTGTAATGGAGTTTGCTTCTCTAAAAATTGGCTTCTTCACATCACATCATCAATGAGATTTCTAAATCTGTACTAATACAGGTACAAGGAATCAATCTGGCGAAGCACTAAAGGTATTAAGGAGAAACTGCTTGCTCGTAACAGCTCCGTGAAGGATCTAAGCAGAGGTGTAAAGCGCGAAGTGAGTGCCGGGATTGCTGGTGTTGCTCGAATGTTTGAGCGTCTAGATTTGACTTCAAAAAGGAATGCTGCATCTGTTGCTCTTTTCAGTTGCAATGGGGGTACATCAAAATCCTCGAAAGGAAAGAACGTAGTGCAAGAGAGTGCTGTCACAGATGATTCAAATAAAATACATAGAATATGTACTGGTTCACCCTCATCACAGGTTTCCCCTACTGTTCCAAGCTCAGTCGAAGTTTCTCTTTCTCAGGTACTGAACTTTATGAAGTTA

mRNA sequence

TGCTGCATCTGCCTCGACCCCTTCACCTCCCACAACCCTCCCACTGTTACCTGTTGCAAGCATGAATATCATCTCCAATGTATTCTTGATTGGTCTCAAAGAAGCAATGAATGCCCAATATGTTGCCAGTTGCTAGTGTTGAAGGATCCTGTTGGCCAGGAGCTTATAGTGGCCATGGCTGGTGAAAGGATTTTAAAGTCAAGAAGCATGTCTTCTGCTGCATCCACTTCTCTGCGATTCCATGAAAACTTTGATGTCGACCACGACTCCTCTCACTCGGATGACTCTGACTTTGATGACCTGATTATGCAGCATCTTGCTGCTGCAGCTAGCAGAGCTCGGTATGTTCATAGAAGCGAGAGGCAAAGGCATTTCGTATCTCCAGAAGGGGTTCCCCAGCAAACATGCCCATTGTCGCCTCGATATCAGGATCCAACTCTAAGTTCACCTATTAGTGATTCGCCATCTCCTGGTTCCATATCTGAGTTTCATGTTCAAGCTTTGTCATCTGTGGGCCTCCATGATGGCAATGAGTTCTCAAGAACTGGAACTAATAAAATATCGCCCAGGTACATCCCCGGACTTGTATATCGACTTCTTTCTTCCATTGATTGCAACCTTAGATTTTCAAGTTGCCCTCAAGCTAAACAAGTAATGATTTCGTTTGTTTTACTGTCTAACATAGATCACACTTCTGTGTCATATAGGGCTTTACTGACTGAGACGTCATCTGGTAGTCAACAGAAAACAAACCAATCTGAGGGGCTCTCTTTCCCTGATTCCATCAAATCCAAAATTTCTTCTACTTCCACAAAGTACAAGGAATCAATCTGGCGAAGCACTAAAGGTATTAAGGAGAAACTGCTTGCTCGTAACAGCTCCGTGAAGGATCTAAGCAGAGGTGTAAAGCGCGAAGTGAGTGCCGGGATTGCTGGTGTTGCTCGAATGTTTGAGCGTCTAGATTTGACTTCAAAAAGGAATGCTGCATCTGTTGCTCTTTTCAGTTGCAATGGGGGTACATCAAAATCCTCGAAAGGAAAGAACGTAGTGCAAGAGAGTGCTGTCACAGATGATTCAAATAAAATACATAGAATATGTACTGGTTCACCCTCATCACAGGTTTCCCCTACTGTTCCAAGCTCAGTCGAAGTTTCTCTTTCTCAGGTACTGAACTTTATGAAGTTA

Coding sequence (CDS)

TGCTGCATCTGCCTCGACCCCTTCACCTCCCACAACCCTCCCACTGTTACCTGTTGCAAGCATGAATATCATCTCCAATGTATTCTTGATTGGTCTCAAAGAAGCAATGAATGCCCAATATGTTGCCAGTTGCTAGTGTTGAAGGATCCTGTTGGCCAGGAGCTTATAGTGGCCATGGCTGGTGAAAGGATTTTAAAGTCAAGAAGCATGTCTTCTGCTGCATCCACTTCTCTGCGATTCCATGAAAACTTTGATGTCGACCACGACTCCTCTCACTCGGATGACTCTGACTTTGATGACCTGATTATGCAGCATCTTGCTGCTGCAGCTAGCAGAGCTCGGTATGTTCATAGAAGCGAGAGGCAAAGGCATTTCGTATCTCCAGAAGGGGTTCCCCAGCAAACATGCCCATTGTCGCCTCGATATCAGGATCCAACTCTAAGTTCACCTATTAGTGATTCGCCATCTCCTGGTTCCATATCTGAGTTTCATGTTCAAGCTTTGTCATCTGTGGGCCTCCATGATGGCAATGAGTTCTCAAGAACTGGAACTAATAAAATATCGCCCAGGTACATCCCCGGACTTGTATATCGACTTCTTTCTTCCATTGATTGCAACCTTAGATTTTCAAGTTGCCCTCAAGCTAAACAAGTAATGATTTCGTTTGTTTTACTGTCTAACATAGATCACACTTCTGTGTCATATAGGGCTTTACTGACTGAGACGTCATCTGGTAGTCAACAGAAAACAAACCAATCTGAGGGGCTCTCTTTCCCTGATTCCATCAAATCCAAAATTTCTTCTACTTCCACAAAGTACAAGGAATCAATCTGGCGAAGCACTAAAGGTATTAAGGAGAAACTGCTTGCTCGTAACAGCTCCGTGAAGGATCTAAGCAGAGGTGTAAAGCGCGAAGTGAGTGCCGGGATTGCTGGTGTTGCTCGAATGTTTGAGCGTCTAGATTTGACTTCAAAAAGGAATGCTGCATCTGTTGCTCTTTTCAGTTGCAATGGGGGTACATCAAAATCCTCGAAAGGAAAGAACGTAGTGCAAGAGAGTGCTGTCACAGATGATTCAAATAAAATACATAGAATATGTACTGGTTCACCCTCATCACAGGTTTCCCCTACTGTTCCAAGCTCAGTCGAAGTTTCTCTTTCTCAGGTACTGAACTTTATGAAGTTA

Protein sequence

CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIVAMAGERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRSERQRHFVSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGNEFSRTGTNKISPRYIPGLVYRLLSSIDCNLRFSSCPQAKQVMISFVLLSNIDHTSVSYRALLTETSSGSQQKTNQSEGLSFPDSIKSKISSTSTKYKESIWRSTKGIKEKLLARNSSVKDLSRGVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVVQESAVTDDSNKIHRICTGSPSSQVSPTVPSSVEVSLSQVLNFMKL

Homology

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BLAST of MS001682 vs. NCBI nr
Match: XP_022138871.1 (E3 ubiquitin-protein ligase RHF1A-like isoform X2 [Momordica charantia])

HSP 1 Score: 634.4 bits (1635), Expect = 6.5e-178
Identity = 339/388 (87.37%), Postives = 340/388 (87.63%), Query Frame = 0


Query: 1   CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIVAMA 60
           CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELI+AMA
Sbjct: 14  CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIMAMA 73

Query: 61  GERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRSE 120
            ERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRSE
Sbjct: 74  SERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRSE 133

Query: 121 RQRHFVSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGNEFS 180
           RQRHFVSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGNEFS
Sbjct: 134 RQRHFVSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGNEFS 193

Query: 181 RTGTNKISPRYIPGLVYRLLSSIDCNLRFSSCPQAKQVMISFVLLSNIDHTSVSYRALLT 240
           RTGTNKISP                                              RALLT
Sbjct: 194 RTGTNKISP----------------------------------------------RALLT 253

Query: 241 ETSSGSQQKTNQSEGLSFPDSIKSKISSTSTKYKESIWRSTKGIKEKLLARNSSVKDLSR 300
           ETSSGSQQKTNQSEGLSFPDSIKSKISSTSTKYKESIWRSTKGIKEKLLARNSSVKDLSR
Sbjct: 254 ETSSGSQQKTNQSEGLSFPDSIKSKISSTSTKYKESIWRSTKGIKEKLLARNSSVKDLSR 313

Query: 301 GVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVVQESAVTDDSN 360
           GVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVVQESAVTDDSN
Sbjct: 314 GVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVVQESAVTDDSN 355

Query: 361 KIHRICTGSPSSQVSPTVPSSVEVSLSQ 389
           KIHRICTGSPSSQVSPTVPSSVEVS SQ
Sbjct: 374 KIHRICTGSPSSQVSPTVPSSVEVSPSQ 355

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BLAST of MS001682 vs. NCBI nr
Match: XP_022138870.1 (E3 ubiquitin-protein ligase RHF1A-like isoform X1 [Momordica charantia])

HSP 1 Score: 629.4 bits (1622), Expect = 2.1e-176
Identity = 339/390 (86.92%), Postives = 340/390 (87.18%), Query Frame = 0


Query: 1   CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIVAMA 60
           CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELI+AMA
Sbjct: 14  CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIMAMA 73

Query: 61  GERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRSE 120
            ERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRSE
Sbjct: 74  SERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRSE 133

Query: 121 RQRHFVSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGNEFS 180
           RQRHFVSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGNEFS
Sbjct: 134 RQRHFVSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGNEFS 193

Query: 181 RTGTNKISPRYIPGLVYRLLSSIDCNLRFSSCPQAKQVMISFVLLSNIDHTSVSYRALLT 240
           RTGTNKISP                                              RALLT
Sbjct: 194 RTGTNKISP----------------------------------------------RALLT 253

Query: 241 ETSSGSQQKTNQSEGLSFPDSIKSKISSTSTK--YKESIWRSTKGIKEKLLARNSSVKDL 300
           ETSSGSQQKTNQSEGLSFPDSIKSKISSTSTK  YKESIWRSTKGIKEKLLARNSSVKDL
Sbjct: 254 ETSSGSQQKTNQSEGLSFPDSIKSKISSTSTKSVYKESIWRSTKGIKEKLLARNSSVKDL 313

Query: 301 SRGVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVVQESAVTDD 360
           SRGVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVVQESAVTDD
Sbjct: 314 SRGVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVVQESAVTDD 357

Query: 361 SNKIHRICTGSPSSQVSPTVPSSVEVSLSQ 389
           SNKIHRICTGSPSSQVSPTVPSSVEVS SQ
Sbjct: 374 SNKIHRICTGSPSSQVSPTVPSSVEVSPSQ 357

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BLAST of MS001682 vs. NCBI nr
Match: XP_022138872.1 (E3 ubiquitin-protein ligase RHF1A-like isoform X3 [Momordica charantia])

HSP 1 Score: 589.3 bits (1518), Expect = 2.4e-164
Identity = 323/390 (82.82%), Postives = 324/390 (83.08%), Query Frame = 0


Query: 1   CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIVAMA 60
           CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELI+AMA
Sbjct: 14  CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIMAMA 73

Query: 61  GERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRSE 120
            ERILKSRSMSSAASTSLRFHENFDVDH                HLAAAASRARYVHRSE
Sbjct: 74  SERILKSRSMSSAASTSLRFHENFDVDH----------------HLAAAASRARYVHRSE 133

Query: 121 RQRHFVSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGNEFS 180
           RQRHFVSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGNEFS
Sbjct: 134 RQRHFVSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGNEFS 193

Query: 181 RTGTNKISPRYIPGLVYRLLSSIDCNLRFSSCPQAKQVMISFVLLSNIDHTSVSYRALLT 240
           RTGTNKISP                                              RALLT
Sbjct: 194 RTGTNKISP----------------------------------------------RALLT 253

Query: 241 ETSSGSQQKTNQSEGLSFPDSIKSKISSTSTK--YKESIWRSTKGIKEKLLARNSSVKDL 300
           ETSSGSQQKTNQSEGLSFPDSIKSKISSTSTK  YKESIWRSTKGIKEKLLARNSSVKDL
Sbjct: 254 ETSSGSQQKTNQSEGLSFPDSIKSKISSTSTKSVYKESIWRSTKGIKEKLLARNSSVKDL 313

Query: 301 SRGVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVVQESAVTDD 360
           SRGVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVVQESAVTDD
Sbjct: 314 SRGVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVVQESAVTDD 341

Query: 361 SNKIHRICTGSPSSQVSPTVPSSVEVSLSQ 389
           SNKIHRICTGSPSSQVSPTVPSSVEVS SQ
Sbjct: 374 SNKIHRICTGSPSSQVSPTVPSSVEVSPSQ 341

[Close]

BLAST of MS001682 vs. NCBI nr
Match: XP_023549293.1 (E3 ubiquitin-protein ligase RHF1A isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 476.5 bits (1225), Expect = 2.3e-130
Identity = 272/398 (68.34%), Postives = 295/398 (74.12%), Query Frame = 0


Query: 1   CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIVAMA 60
           CCICLDPFTSH+PP++T CKHEYHLQCILDWSQRSNECPICCQLLVL+DPVGQEL+ A+A
Sbjct: 25  CCICLDPFTSHDPPSITSCKHEYHLQCILDWSQRSNECPICCQLLVLRDPVGQELLAAIA 84

Query: 61  GERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRSE 120
            E++LKSRS+SS ASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHR E
Sbjct: 85  SEKLLKSRSLSSVASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRRE 144

Query: 121 RQRHF---------VSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSV 180
           RQRHF           PE VP   CPLSPR+QD TLSSP SDSPSPGS        LSS 
Sbjct: 145 RQRHFGLGPSQGASACPEEVPVLICPLSPRFQDATLSSPNSDSPSPGS-------PLSSG 204

Query: 181 GLHDGNEFSRTGTNKISPRYIPGLVYRLLSSIDCNLRFSSCPQAKQVMISFVLLSNIDHT 240
           G        RTG +K++P                                          
Sbjct: 205 G--------RTGISKVTP------------------------------------------ 264

Query: 241 SVSYRALLTETSSGSQQKTNQSEGLSFPDSIKSKISSTSTKYKESIWRSTKGIKEKLLAR 300
               R LLTETSSGSQQKTNQSEGLSFPDSIKSK S+TS KYKESIWRST+GIKEKLLAR
Sbjct: 265 ----RVLLTETSSGSQQKTNQSEGLSFPDSIKSKWSATSAKYKESIWRSTQGIKEKLLAR 324

Query: 301 NSSVKDLSRGVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVV- 360
           NSSVK+LSRGVKREVSAGIAGVARM +RLDL+SKRNAASV+ FSC+GGTS S KGKNVV 
Sbjct: 325 NSSVKELSRGVKREVSAGIAGVARMIDRLDLSSKRNAASVSFFSCSGGTSNSMKGKNVVM 360

Query: 361 QESAVTDDSNKIHRICTGSPSSQVSPTVPSSVEVSLSQ 389
           QES VTD S K  RICTGSP S VSPTVP +V+ SL+Q
Sbjct: 385 QESGVTDGSEKNDRICTGSP-SHVSPTVPGTVDDSLAQ 360

[Close]

BLAST of MS001682 vs. NCBI nr
Match: XP_023006440.1 (E3 ubiquitin-protein ligase RHF1A isoform X1 [Cucurbita maxima])

HSP 1 Score: 473.0 bits (1216), Expect = 2.5e-129
Identity = 273/398 (68.59%), Postives = 292/398 (73.37%), Query Frame = 0


Query: 1   CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIVAMA 60
           CCICLDPFTSH+PP++T CKHEYHLQCILDWSQRSNECPICCQLLVL+DPVGQEL+ A+A
Sbjct: 25  CCICLDPFTSHDPPSITSCKHEYHLQCILDWSQRSNECPICCQLLVLRDPVGQELLAAIA 84

Query: 61  GERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRSE 120
            E++LKSRS+SS ASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHR E
Sbjct: 85  SEKLLKSRSLSSVASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRRE 144

Query: 121 RQRHF---------VSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSV 180
           RQRHF           PE VP   CPLSPR+QD TLSSP SDSPSPGS        LSS 
Sbjct: 145 RQRHFGLGPSQGVSACPEEVPVLICPLSPRFQDATLSSPNSDSPSPGS-------PLSSG 204

Query: 181 GLHDGNEFSRTGTNKISPRYIPGLVYRLLSSIDCNLRFSSCPQAKQVMISFVLLSNIDHT 240
           G        RTG +KISP                                          
Sbjct: 205 G--------RTGISKISP------------------------------------------ 264

Query: 241 SVSYRALLTETSSGSQQKTNQSEGLSFPDSIKSKISSTSTKYKESIWRSTKGIKEKLLAR 300
               R LLTET SGSQQKTNQ EGLSFPDSIKSK S+TS KYKESIWRST+GIKEKLLAR
Sbjct: 265 ----RVLLTETLSGSQQKTNQPEGLSFPDSIKSKWSATSAKYKESIWRSTQGIKEKLLAR 324

Query: 301 NSSVKDLSRGVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVV- 360
           NSSVK+LSRGVKREVSAGIAGVARM +RLDL+SKRNAASV+ FSC+GGTS S KGKNVV 
Sbjct: 325 NSSVKELSRGVKREVSAGIAGVARMIDRLDLSSKRNAASVSFFSCSGGTSNSMKGKNVVM 360

Query: 361 QESAVTDDSNKIHRICTGSPSSQVSPTVPSSVEVSLSQ 389
           QES VTD S K  RICTGSP S VSPTVP SV  SL+Q
Sbjct: 385 QESGVTDGSVKNDRICTGSP-SHVSPTVPGSVNDSLAQ 360

[Close]

BLAST of MS001682 vs. ExPASy Swiss-Prot
Match: Q4TU14 (E3 ubiquitin-protein ligase RHF1A OS=Arabidopsis thaliana OX=3702 GN=RHF1A PE=1 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 4.5e-49
Identity = 139/364 (38.19%), Postives = 196/364 (53.85%), Query Frame = 0


Query: 1   CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIVAMA 60
           C ICL+PFT  +P TVT CKHEYHLQCI++WSQRS ECPIC QL VL+DP  QEL+ A+ 
Sbjct: 46  CSICLEPFTLQDPSTVTSCKHEYHLQCIIEWSQRSKECPICWQLFVLRDPASQELLAAVE 105

Query: 61  GERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRSE 120
            ER+LK+R++SS++  S+  H + D   +   S  S FD+  ++HL  AA R   + R +
Sbjct: 106 KERLLKTRNISSSSPISIH-HSHDDFHSEEEESQFSSFDEQFLRHLTEAAHRRCLLRRRD 165

Query: 121 RQRHFVSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGNEFS 180
            Q   +S   V            DPT   P         ++ + + A+S V   + N   
Sbjct: 166 GQ---ISSSLVSS---------SDPTTIHPTD------LVNLYRLSAISHVEHQNSNPCP 225

Query: 181 RTGTNKISPRYIPGLVYRLLSSIDCNLRFSSCPQAKQVMISFVLLSNIDHTSVSYRALLT 240
             G+  ++P  + G               SS P                 ++   R    
Sbjct: 226 SPGS--MTPSPVSG--------------HSSIPA---------------DSNNGSRISPG 285

Query: 241 ETSSGSQQKTNQSEGLSFPDSIKSKISSTSTKYKESIWRSTKGIKEKLLARNSSVKDLSR 300
            + S S Q     E  S P++IKSK+++ S KYKESI +S +G+KEKLLARN+SVK+LS+
Sbjct: 286 PSPSRSSQSPKSPEASSLPEAIKSKLAAASAKYKESISKSKQGLKEKLLARNNSVKELSK 345

Query: 301 GVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCN---GGTSKSSKGKNVVQESAVTD 360
           GV+RE++AGIAGVARM ER+D +SKR   S  + +      G + S KGK V   S   +
Sbjct: 346 GVQREMNAGIAGVARMIERMDFSSKRFGGSAHVSTSTATASGFNFSFKGKRVEANSKSNN 359

Query: 361 DSNK 362
           + +K
Sbjct: 406 NGDK 359

[Close]

BLAST of MS001682 vs. ExPASy Swiss-Prot
Match: Q9ZT42 (E3 ubiquitin-protein ligase RHF2A OS=Arabidopsis thaliana OX=3702 GN=RHF2A PE=2 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.8e-37
Identity = 131/379 (34.56%), Postives = 190/379 (50.13%), Query Frame = 0


Query: 1   CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIVAMA 60
           C ICL+ F   +P T+T CKHEYHLQCIL+W QRS++CP+C Q + LKDP  QEL+ A+ 
Sbjct: 33  CSICLESFCESDPSTLTSCKHEYHLQCILEWCQRSSQCPMCWQSISLKDPTSQELLEAVE 92

Query: 61  GERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAAS--RARYVHR 120
            ER  +     +A         +F++ H     D+++ ++ I+QHLAAAA+  RAR+  R
Sbjct: 93  QERNFRFNPTRNATIFRHPTLGDFELQHLPVGVDNAEIEERIIQHLAAAAAMGRARHGVR 152

Query: 121 SERQRHFVSPEGVPQ-QTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGN 180
            E  R   S +G  Q       P    P    P+  SPS    S+  V  L    L +G+
Sbjct: 153 REGHRSRSSSQGHQQFMVFSSQPNASSPPPHPPMPSSPSQRDESD-TVSNLPHNALGEGS 212

Query: 181 EFSRTGTNKIS-PRYIPGLVYRLLSSIDCNLRFSSCPQAKQVMISFVLLSNIDHTSVSYR 240
             S T     S PR +        S+ D N R                            
Sbjct: 213 HQSNTQPPTSSHPRQVSP------SASDSNSR---------------------------- 272

Query: 241 ALLTETSSGSQQKTNQSEGLSFPDSIKSKISSTSTKYKESIWRSTKGIKEKLLARNSSVK 300
             L ++S   Q +   SE  SF +S+KS++++ ST+YKESI ++T+  K++L +RN+S+ 
Sbjct: 273 -PLNQSSPSEQDRAGPSELQSFSESLKSRLNAVSTRYKESISKNTRNWKDRLFSRNTSMA 332

Query: 301 DLSRGVKREVSAGIAGVARMFERLDL--TSKRNAASVALFSCNGGTSKSSKGKNVVQESA 360
           DL   VKREVSAGIA V+RM ERL+    S+ + ASV+  S N     +++         
Sbjct: 333 DLGSEVKREVSAGIATVSRMMERLETRENSRPSTASVSDVSENHTPETNNEHNRAAAGDE 375

Query: 361 VTDDSNKIHRIC-TGSPSS 373
            + +   +   C TGS SS
Sbjct: 393 HSVNERGVKETCATGSGSS 375

[Close]

BLAST of MS001682 vs. ExPASy Swiss-Prot
Match: Q8LE94 (E3 ubiquitin-protein ligase At3g02290 OS=Arabidopsis thaliana OX=3702 GN=At3g02290 PE=2 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.5e-07
Identity = 21/45 (46.67%), Postives = 30/45 (66.67%), Query Frame = 0


Query: 1   CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLL 46
           C  CL+ +TS NP  VT C H +HL CI +W +RS  CP+C +++
Sbjct: 181 CPTCLEEYTSENPKIVTKCSHHFHLSCIYEWMERSENCPVCGKVM 225

[Close]

BLAST of MS001682 vs. ExPASy Swiss-Prot
Match: Q2HIJ8 (Probable E3 ubiquitin-protein ligase RHB1A OS=Arabidopsis thaliana OX=3702 GN=RHB1A PE=2 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 2.5e-07
Identity = 22/49 (44.90%), Postives = 32/49 (65.31%), Query Frame = 0


Query: 1   CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKD 50
           C IC + +   NP   T C+HE+HL C+L+W +RS+ CPIC + +V  D
Sbjct: 139 CPICFEDYDVENPRLTTKCEHEFHLSCLLEWIERSDRCPICDKEVVFDD 187

[Close]

BLAST of MS001682 vs. ExPASy Swiss-Prot
Match: Q93ZF6 (E3 ubiquitin-protein ligase AIRP1 OS=Arabidopsis thaliana OX=3702 GN=AIRP1 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 5.6e-07
Identity = 23/49 (46.94%), Postives = 32/49 (65.31%), Query Frame = 0


Query: 1   CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKD 50
           C ICL+ +   NP  +T C H++HL CIL W +RS  CP+C + LVL +
Sbjct: 104 CPICLEEYEIDNPKLLTKCGHDFHLACILAWMERSEACPVCDKELVLTE 152

[Close]

BLAST of MS001682 vs. ExPASy TrEMBL
Match: A0A6J1CAZ3 (E3 ubiquitin-protein ligase RHF1A-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111009932 PE=4 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 3.1e-178
Identity = 339/388 (87.37%), Postives = 340/388 (87.63%), Query Frame = 0


Query: 1   CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIVAMA 60
           CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELI+AMA
Sbjct: 14  CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIMAMA 73

Query: 61  GERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRSE 120
            ERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRSE
Sbjct: 74  SERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRSE 133

Query: 121 RQRHFVSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGNEFS 180
           RQRHFVSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGNEFS
Sbjct: 134 RQRHFVSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGNEFS 193

Query: 181 RTGTNKISPRYIPGLVYRLLSSIDCNLRFSSCPQAKQVMISFVLLSNIDHTSVSYRALLT 240
           RTGTNKISP                                              RALLT
Sbjct: 194 RTGTNKISP----------------------------------------------RALLT 253

Query: 241 ETSSGSQQKTNQSEGLSFPDSIKSKISSTSTKYKESIWRSTKGIKEKLLARNSSVKDLSR 300
           ETSSGSQQKTNQSEGLSFPDSIKSKISSTSTKYKESIWRSTKGIKEKLLARNSSVKDLSR
Sbjct: 254 ETSSGSQQKTNQSEGLSFPDSIKSKISSTSTKYKESIWRSTKGIKEKLLARNSSVKDLSR 313

Query: 301 GVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVVQESAVTDDSN 360
           GVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVVQESAVTDDSN
Sbjct: 314 GVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVVQESAVTDDSN 355

Query: 361 KIHRICTGSPSSQVSPTVPSSVEVSLSQ 389
           KIHRICTGSPSSQVSPTVPSSVEVS SQ
Sbjct: 374 KIHRICTGSPSSQVSPTVPSSVEVSPSQ 355

[Close]

BLAST of MS001682 vs. ExPASy TrEMBL
Match: A0A6J1CAQ5 (E3 ubiquitin-protein ligase RHF1A-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009932 PE=4 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 1.0e-176
Identity = 339/390 (86.92%), Postives = 340/390 (87.18%), Query Frame = 0


Query: 1   CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIVAMA 60
           CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELI+AMA
Sbjct: 14  CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIMAMA 73

Query: 61  GERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRSE 120
            ERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRSE
Sbjct: 74  SERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRSE 133

Query: 121 RQRHFVSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGNEFS 180
           RQRHFVSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGNEFS
Sbjct: 134 RQRHFVSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGNEFS 193

Query: 181 RTGTNKISPRYIPGLVYRLLSSIDCNLRFSSCPQAKQVMISFVLLSNIDHTSVSYRALLT 240
           RTGTNKISP                                              RALLT
Sbjct: 194 RTGTNKISP----------------------------------------------RALLT 253

Query: 241 ETSSGSQQKTNQSEGLSFPDSIKSKISSTSTK--YKESIWRSTKGIKEKLLARNSSVKDL 300
           ETSSGSQQKTNQSEGLSFPDSIKSKISSTSTK  YKESIWRSTKGIKEKLLARNSSVKDL
Sbjct: 254 ETSSGSQQKTNQSEGLSFPDSIKSKISSTSTKSVYKESIWRSTKGIKEKLLARNSSVKDL 313

Query: 301 SRGVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVVQESAVTDD 360
           SRGVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVVQESAVTDD
Sbjct: 314 SRGVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVVQESAVTDD 357

Query: 361 SNKIHRICTGSPSSQVSPTVPSSVEVSLSQ 389
           SNKIHRICTGSPSSQVSPTVPSSVEVS SQ
Sbjct: 374 SNKIHRICTGSPSSQVSPTVPSSVEVSPSQ 357

[Close]

BLAST of MS001682 vs. ExPASy TrEMBL
Match: A0A6J1CCF2 (E3 ubiquitin-protein ligase RHF1A-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111009932 PE=4 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 1.2e-164
Identity = 323/390 (82.82%), Postives = 324/390 (83.08%), Query Frame = 0


Query: 1   CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIVAMA 60
           CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELI+AMA
Sbjct: 14  CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIMAMA 73

Query: 61  GERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRSE 120
            ERILKSRSMSSAASTSLRFHENFDVDH                HLAAAASRARYVHRSE
Sbjct: 74  SERILKSRSMSSAASTSLRFHENFDVDH----------------HLAAAASRARYVHRSE 133

Query: 121 RQRHFVSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGNEFS 180
           RQRHFVSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGNEFS
Sbjct: 134 RQRHFVSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGNEFS 193

Query: 181 RTGTNKISPRYIPGLVYRLLSSIDCNLRFSSCPQAKQVMISFVLLSNIDHTSVSYRALLT 240
           RTGTNKISP                                              RALLT
Sbjct: 194 RTGTNKISP----------------------------------------------RALLT 253

Query: 241 ETSSGSQQKTNQSEGLSFPDSIKSKISSTSTK--YKESIWRSTKGIKEKLLARNSSVKDL 300
           ETSSGSQQKTNQSEGLSFPDSIKSKISSTSTK  YKESIWRSTKGIKEKLLARNSSVKDL
Sbjct: 254 ETSSGSQQKTNQSEGLSFPDSIKSKISSTSTKSVYKESIWRSTKGIKEKLLARNSSVKDL 313

Query: 301 SRGVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVVQESAVTDD 360
           SRGVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVVQESAVTDD
Sbjct: 314 SRGVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVVQESAVTDD 341

Query: 361 SNKIHRICTGSPSSQVSPTVPSSVEVSLSQ 389
           SNKIHRICTGSPSSQVSPTVPSSVEVS SQ
Sbjct: 374 SNKIHRICTGSPSSQVSPTVPSSVEVSPSQ 341

[Close]

BLAST of MS001682 vs. ExPASy TrEMBL
Match: A0A6J1L048 (E3 ubiquitin-protein ligase RHF1A isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499166 PE=4 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 1.2e-129
Identity = 273/398 (68.59%), Postives = 292/398 (73.37%), Query Frame = 0


Query: 1   CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIVAMA 60
           CCICLDPFTSH+PP++T CKHEYHLQCILDWSQRSNECPICCQLLVL+DPVGQEL+ A+A
Sbjct: 25  CCICLDPFTSHDPPSITSCKHEYHLQCILDWSQRSNECPICCQLLVLRDPVGQELLAAIA 84

Query: 61  GERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRSE 120
            E++LKSRS+SS ASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHR E
Sbjct: 85  SEKLLKSRSLSSVASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRRE 144

Query: 121 RQRHF---------VSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSV 180
           RQRHF           PE VP   CPLSPR+QD TLSSP SDSPSPGS        LSS 
Sbjct: 145 RQRHFGLGPSQGVSACPEEVPVLICPLSPRFQDATLSSPNSDSPSPGS-------PLSSG 204

Query: 181 GLHDGNEFSRTGTNKISPRYIPGLVYRLLSSIDCNLRFSSCPQAKQVMISFVLLSNIDHT 240
           G        RTG +KISP                                          
Sbjct: 205 G--------RTGISKISP------------------------------------------ 264

Query: 241 SVSYRALLTETSSGSQQKTNQSEGLSFPDSIKSKISSTSTKYKESIWRSTKGIKEKLLAR 300
               R LLTET SGSQQKTNQ EGLSFPDSIKSK S+TS KYKESIWRST+GIKEKLLAR
Sbjct: 265 ----RVLLTETLSGSQQKTNQPEGLSFPDSIKSKWSATSAKYKESIWRSTQGIKEKLLAR 324

Query: 301 NSSVKDLSRGVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVV- 360
           NSSVK+LSRGVKREVSAGIAGVARM +RLDL+SKRNAASV+ FSC+GGTS S KGKNVV 
Sbjct: 325 NSSVKELSRGVKREVSAGIAGVARMIDRLDLSSKRNAASVSFFSCSGGTSNSMKGKNVVM 360

Query: 361 QESAVTDDSNKIHRICTGSPSSQVSPTVPSSVEVSLSQ 389
           QES VTD S K  RICTGSP S VSPTVP SV  SL+Q
Sbjct: 385 QESGVTDGSVKNDRICTGSP-SHVSPTVPGSVNDSLAQ 360

[Close]

BLAST of MS001682 vs. ExPASy TrEMBL
Match: A0A6J1H5S5 (E3 ubiquitin-protein ligase RHF1A isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111460372 PE=4 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 7.8e-129
Identity = 272/398 (68.34%), Postives = 293/398 (73.62%), Query Frame = 0


Query: 1   CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIVAMA 60
           CCICLDPFTSH+PP++T CKHEYHLQCILDWSQRSNECPICCQLLVL+DPVGQEL+ A+A
Sbjct: 25  CCICLDPFTSHDPPSITSCKHEYHLQCILDWSQRSNECPICCQLLVLRDPVGQELLAAIA 84

Query: 61  GERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRSE 120
            E++LKSRS+SS ASTSLRFHENFDVDHDSS SDDSDFDDLIMQHLAAAASRARYVHR E
Sbjct: 85  NEKLLKSRSLSSVASTSLRFHENFDVDHDSSRSDDSDFDDLIMQHLAAAASRARYVHRRE 144

Query: 121 RQRHF---------VSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSV 180
           RQR F           PE VP   CPLSPR+QD +LSSP SDSPSPGS        LSS 
Sbjct: 145 RQRQFGLGPSQGASACPEEVPVLICPLSPRFQDASLSSPNSDSPSPGS-------PLSSG 204

Query: 181 GLHDGNEFSRTGTNKISPRYIPGLVYRLLSSIDCNLRFSSCPQAKQVMISFVLLSNIDHT 240
           G        RTG +KISP                                          
Sbjct: 205 G--------RTGISKISP------------------------------------------ 264

Query: 241 SVSYRALLTETSSGSQQKTNQSEGLSFPDSIKSKISSTSTKYKESIWRSTKGIKEKLLAR 300
               R LLTETSSGSQQKTNQSEGLSFPDSIKSK S+TS KYKESIWRST+GIKEKLLAR
Sbjct: 265 ----RVLLTETSSGSQQKTNQSEGLSFPDSIKSKWSATSAKYKESIWRSTQGIKEKLLAR 324

Query: 301 NSSVKDLSRGVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCNGGTSKSSKGKNVV- 360
           NSSVK+LSRGVKREVSAGIAGVARM +RLDL+SKRNAASV+ FSC+GGTS S KGKNVV 
Sbjct: 325 NSSVKELSRGVKREVSAGIAGVARMIDRLDLSSKRNAASVSFFSCSGGTSNSMKGKNVVM 360

Query: 361 QESAVTDDSNKIHRICTGSPSSQVSPTVPSSVEVSLSQ 389
           QES VTD S K  RICTGSP S VSPTVP SV+ SL+Q
Sbjct: 385 QESGVTDGSVKNDRICTGSP-SHVSPTVPGSVDDSLAQ 360

[Close]

BLAST of MS001682 vs. TAIR 10
Match: AT4G14220.1 (RING-H2 group F1A )

HSP 1 Score: 196.8 bits (499), Expect = 3.2e-50
Identity = 139/364 (38.19%), Postives = 196/364 (53.85%), Query Frame = 0


Query: 1   CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIVAMA 60
           C ICL+PFT  +P TVT CKHEYHLQCI++WSQRS ECPIC QL VL+DP  QEL+ A+ 
Sbjct: 46  CSICLEPFTLQDPSTVTSCKHEYHLQCIIEWSQRSKECPICWQLFVLRDPASQELLAAVE 105

Query: 61  GERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRSE 120
            ER+LK+R++SS++  S+  H + D   +   S  S FD+  ++HL  AA R   + R +
Sbjct: 106 KERLLKTRNISSSSPISIH-HSHDDFHSEEEESQFSSFDEQFLRHLTEAAHRRCLLRRRD 165

Query: 121 RQRHFVSPEGVPQQTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGNEFS 180
            Q   +S   V            DPT   P         ++ + + A+S V   + N   
Sbjct: 166 GQ---ISSSLVSS---------SDPTTIHPTD------LVNLYRLSAISHVEHQNSNPCP 225

Query: 181 RTGTNKISPRYIPGLVYRLLSSIDCNLRFSSCPQAKQVMISFVLLSNIDHTSVSYRALLT 240
             G+  ++P  + G               SS P                 ++   R    
Sbjct: 226 SPGS--MTPSPVSG--------------HSSIPA---------------DSNNGSRISPG 285

Query: 241 ETSSGSQQKTNQSEGLSFPDSIKSKISSTSTKYKESIWRSTKGIKEKLLARNSSVKDLSR 300
            + S S Q     E  S P++IKSK+++ S KYKESI +S +G+KEKLLARN+SVK+LS+
Sbjct: 286 PSPSRSSQSPKSPEASSLPEAIKSKLAAASAKYKESISKSKQGLKEKLLARNNSVKELSK 345

Query: 301 GVKREVSAGIAGVARMFERLDLTSKRNAASVALFSCN---GGTSKSSKGKNVVQESAVTD 360
           GV+RE++AGIAGVARM ER+D +SKR   S  + +      G + S KGK V   S   +
Sbjct: 346 GVQREMNAGIAGVARMIERMDFSSKRFGGSAHVSTSTATASGFNFSFKGKRVEANSKSNN 359

Query: 361 DSNK 362
           + +K
Sbjct: 406 NGDK 359

[Close]

BLAST of MS001682 vs. TAIR 10
Match: AT5G22000.2 (RING-H2 group F2A )

HSP 1 Score: 158.3 bits (399), Expect = 1.3e-38
Identity = 131/379 (34.56%), Postives = 190/379 (50.13%), Query Frame = 0


Query: 1   CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIVAMA 60
           C ICL+ F   +P T+T CKHEYHLQCIL+W QRS++CP+C Q + LKDP  QEL+ A+ 
Sbjct: 33  CSICLESFCESDPSTLTSCKHEYHLQCILEWCQRSSQCPMCWQSISLKDPTSQELLEAVE 92

Query: 61  GERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAAS--RARYVHR 120
            ER  +     +A         +F++ H     D+++ ++ I+QHLAAAA+  RAR+  R
Sbjct: 93  QERNFRFNPTRNATIFRHPTLGDFELQHLPVGVDNAEIEERIIQHLAAAAAMGRARHGVR 152

Query: 121 SERQRHFVSPEGVPQ-QTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGN 180
            E  R   S +G  Q       P    P    P+  SPS    S+  V  L    L +G+
Sbjct: 153 REGHRSRSSSQGHQQFMVFSSQPNASSPPPHPPMPSSPSQRDESD-TVSNLPHNALGEGS 212

Query: 181 EFSRTGTNKIS-PRYIPGLVYRLLSSIDCNLRFSSCPQAKQVMISFVLLSNIDHTSVSYR 240
             S T     S PR +        S+ D N R                            
Sbjct: 213 HQSNTQPPTSSHPRQVSP------SASDSNSR---------------------------- 272

Query: 241 ALLTETSSGSQQKTNQSEGLSFPDSIKSKISSTSTKYKESIWRSTKGIKEKLLARNSSVK 300
             L ++S   Q +   SE  SF +S+KS++++ ST+YKESI ++T+  K++L +RN+S+ 
Sbjct: 273 -PLNQSSPSEQDRAGPSELQSFSESLKSRLNAVSTRYKESISKNTRNWKDRLFSRNTSMA 332

Query: 301 DLSRGVKREVSAGIAGVARMFERLDL--TSKRNAASVALFSCNGGTSKSSKGKNVVQESA 360
           DL   VKREVSAGIA V+RM ERL+    S+ + ASV+  S N     +++         
Sbjct: 333 DLGSEVKREVSAGIATVSRMMERLETRENSRPSTASVSDVSENHTPETNNEHNRAAAGDE 375

Query: 361 VTDDSNKIHRIC-TGSPSS 373
            + +   +   C TGS SS
Sbjct: 393 HSVNERGVKETCATGSGSS 375

[Close]

BLAST of MS001682 vs. TAIR 10
Match: AT5G22000.1 (RING-H2 group F2A )

HSP 1 Score: 158.3 bits (399), Expect = 1.3e-38
Identity = 131/379 (34.56%), Postives = 190/379 (50.13%), Query Frame = 0


Query: 1   CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIVAMA 60
           C ICL+ F   +P T+T CKHEYHLQCIL+W QRS++CP+C Q + LKDP  QEL+ A+ 
Sbjct: 33  CSICLESFCESDPSTLTSCKHEYHLQCILEWCQRSSQCPMCWQSISLKDPTSQELLEAVE 92

Query: 61  GERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAAS--RARYVHR 120
            ER  +     +A         +F++ H     D+++ ++ I+QHLAAAA+  RAR+  R
Sbjct: 93  QERNFRFNPTRNATIFRHPTLGDFELQHLPVGVDNAEIEERIIQHLAAAAAMGRARHGVR 152

Query: 121 SERQRHFVSPEGVPQ-QTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGN 180
            E  R   S +G  Q       P    P    P+  SPS    S+  V  L    L +G+
Sbjct: 153 REGHRSRSSSQGHQQFMVFSSQPNASSPPPHPPMPSSPSQRDESD-TVSNLPHNALGEGS 212

Query: 181 EFSRTGTNKIS-PRYIPGLVYRLLSSIDCNLRFSSCPQAKQVMISFVLLSNIDHTSVSYR 240
             S T     S PR +        S+ D N R                            
Sbjct: 213 HQSNTQPPTSSHPRQVSP------SASDSNSR---------------------------- 272

Query: 241 ALLTETSSGSQQKTNQSEGLSFPDSIKSKISSTSTKYKESIWRSTKGIKEKLLARNSSVK 300
             L ++S   Q +   SE  SF +S+KS++++ ST+YKESI ++T+  K++L +RN+S+ 
Sbjct: 273 -PLNQSSPSEQDRAGPSELQSFSESLKSRLNAVSTRYKESISKNTRNWKDRLFSRNTSMA 332

Query: 301 DLSRGVKREVSAGIAGVARMFERLDL--TSKRNAASVALFSCNGGTSKSSKGKNVVQESA 360
           DL   VKREVSAGIA V+RM ERL+    S+ + ASV+  S N     +++         
Sbjct: 333 DLGSEVKREVSAGIATVSRMMERLETRENSRPSTASVSDVSENHTPETNNEHNRAAAGDE 375

Query: 361 VTDDSNKIHRIC-TGSPSS 373
            + +   +   C TGS SS
Sbjct: 393 HSVNERGVKETCATGSGSS 375

[Close]

BLAST of MS001682 vs. TAIR 10
Match: AT5G22000.3 (RING-H2 group F2A )

HSP 1 Score: 158.3 bits (399), Expect = 1.3e-38
Identity = 131/379 (34.56%), Postives = 190/379 (50.13%), Query Frame = 0


Query: 1   CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLLVLKDPVGQELIVAMA 60
           C ICL+ F   +P T+T CKHEYHLQCIL+W QRS++CP+C Q + LKDP  QEL+ A+ 
Sbjct: 33  CSICLESFCESDPSTLTSCKHEYHLQCILEWCQRSSQCPMCWQSISLKDPTSQELLEAVE 92

Query: 61  GERILKSRSMSSAASTSLRFHENFDVDHDSSHSDDSDFDDLIMQHLAAAAS--RARYVHR 120
            ER  +     +A         +F++ H     D+++ ++ I+QHLAAAA+  RAR+  R
Sbjct: 93  QERNFRFNPTRNATIFRHPTLGDFELQHLPVGVDNAEIEERIIQHLAAAAAMGRARHGVR 152

Query: 121 SERQRHFVSPEGVPQ-QTCPLSPRYQDPTLSSPISDSPSPGSISEFHVQALSSVGLHDGN 180
            E  R   S +G  Q       P    P    P+  SPS    S+  V  L    L +G+
Sbjct: 153 REGHRSRSSSQGHQQFMVFSSQPNASSPPPHPPMPSSPSQRDESD-TVSNLPHNALGEGS 212

Query: 181 EFSRTGTNKIS-PRYIPGLVYRLLSSIDCNLRFSSCPQAKQVMISFVLLSNIDHTSVSYR 240
             S T     S PR +        S+ D N R                            
Sbjct: 213 HQSNTQPPTSSHPRQVSP------SASDSNSR---------------------------- 272

Query: 241 ALLTETSSGSQQKTNQSEGLSFPDSIKSKISSTSTKYKESIWRSTKGIKEKLLARNSSVK 300
             L ++S   Q +   SE  SF +S+KS++++ ST+YKESI ++T+  K++L +RN+S+ 
Sbjct: 273 -PLNQSSPSEQDRAGPSELQSFSESLKSRLNAVSTRYKESISKNTRNWKDRLFSRNTSMA 332

Query: 301 DLSRGVKREVSAGIAGVARMFERLDL--TSKRNAASVALFSCNGGTSKSSKGKNVVQESA 360
           DL   VKREVSAGIA V+RM ERL+    S+ + ASV+  S N     +++         
Sbjct: 333 DLGSEVKREVSAGIATVSRMMERLETRENSRPSTASVSDVSENHTPETNNEHNRAAAGDE 375

Query: 361 VTDDSNKIHRIC-TGSPSS 373
            + +   +   C TGS SS
Sbjct: 393 HSVNERGVKETCATGSGSS 375

[Close]

BLAST of MS001682 vs. TAIR 10
Match: AT3G02290.1 (RING/U-box superfamily protein )

HSP 1 Score: 58.9 bits (141), Expect = 1.0e-08
Identity = 21/45 (46.67%), Postives = 30/45 (66.67%), Query Frame = 0


Query: 1   CCICLDPFTSHNPPTVTCCKHEYHLQCILDWSQRSNECPICCQLL 46
           C  CL+ +TS NP  VT C H +HL CI +W +RS  CP+C +++
Sbjct: 181 CPTCLEEYTSENPKIVTKCSHHFHLSCIYEWMERSENCPVCGKVM 225

The following BLAST results are available for this feature:

BLAST of MS001682 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022138871.1	6.5e-178	87.37	E3 ubiquitin-protein ligase RHF1A-like isoform X2 [Momordica charantia]	[more]
XP_022138870.1	2.1e-176	86.92	E3 ubiquitin-protein ligase RHF1A-like isoform X1 [Momordica charantia]	[more]
XP_022138872.1	2.4e-164	82.82	E3 ubiquitin-protein ligase RHF1A-like isoform X3 [Momordica charantia]	[more]
XP_023549293.1	2.3e-130	68.34	E3 ubiquitin-protein ligase RHF1A isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_023006440.1	2.5e-129	68.59	E3 ubiquitin-protein ligase RHF1A isoform X1 [Cucurbita maxima]	[more]

BLAST of MS001682 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q4TU14	4.5e-49	38.19	E3 ubiquitin-protein ligase RHF1A OS=Arabidopsis thaliana OX=3702 GN=RHF1A PE=1 ...	[more]
Q9ZT42	1.8e-37	34.56	E3 ubiquitin-protein ligase RHF2A OS=Arabidopsis thaliana OX=3702 GN=RHF2A PE=2 ...	[more]
Q8LE94	1.5e-07	46.67	E3 ubiquitin-protein ligase At3g02290 OS=Arabidopsis thaliana OX=3702 GN=At3g022...	[more]
Q2HIJ8	2.5e-07	44.90	Probable E3 ubiquitin-protein ligase RHB1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q93ZF6	5.6e-07	46.94	E3 ubiquitin-protein ligase AIRP1 OS=Arabidopsis thaliana OX=3702 GN=AIRP1 PE=1 ...	[more]

BLAST of MS001682 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CAZ3	3.1e-178	87.37	E3 ubiquitin-protein ligase RHF1A-like isoform X2 OS=Momordica charantia OX=3673...	[more]
A0A6J1CAQ5	1.0e-176	86.92	E3 ubiquitin-protein ligase RHF1A-like isoform X1 OS=Momordica charantia OX=3673...	[more]
A0A6J1CCF2	1.2e-164	82.82	E3 ubiquitin-protein ligase RHF1A-like isoform X3 OS=Momordica charantia OX=3673...	[more]
A0A6J1L048	1.2e-129	68.59	E3 ubiquitin-protein ligase RHF1A isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1H5S5	7.8e-129	68.34	E3 ubiquitin-protein ligase RHF1A isoform X1 OS=Cucurbita moschata OX=3662 GN=LO...	[more]

BLAST of MS001682 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G14220.1	3.2e-50	38.19	RING-H2 group F1A	[more]
AT5G22000.2	1.3e-38	34.56	RING-H2 group F2A	[more]
AT5G22000.1	1.3e-38	34.56	RING-H2 group F2A	[more]
AT5G22000.3	1.3e-38	34.56	RING-H2 group F2A	[more]
AT3G02290.1	1.0e-08	46.67	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 1..41 e-value: 3.5E-6 score: 36.6
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 1..41 e-value: 2.8E-6 score: 27.6
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 1..41 score: 11.545086
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 1..145 e-value: 7.3E-11 score: 44.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 123..158
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 128..158
None	No IPR available	PANTHER	PTHR46463	ZINC FINGER, RING/FYVE/PHD-TYPE	coord: 1..367
None	No IPR available	PANTHER	PTHR46463:SF16	E3 UBIQUITIN-PROTEIN LIGASE RHF1A	coord: 1..367
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 1..48

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS001682.1	MS001682.1	mRNA