MS001640 (gene) Bitter gourd (TR) v1

Overview


Name	MS001640
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal
Location	scaffold377: 337826 .. 339638 (-)
RNA-Seq Expression	MS001640
Synteny	MS001640

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAGGCTACAAATTCGTAAAAATCGTCCGTCAGAATCGAAGCTCCGCCGTGTTCCACCTCCGCCTCCACCGCCCCTCCCACCACAATGCCTTCACCACCGACCTACTCTTCGAATTCCGTCGAGCCCTCTCTTCTCTTGACCAAAACCCCGACGCTCGCGTCATCGTCCTCTCCGGATCCGGCAAAAATTTCTGCGCCGGAATCGATCTCAAATCCCTTGCATCCATTTTCCAGAAATTTCAGTCCGGCGACCGCGGCCGCGCCGGCGAGCAGCTCCGGCGGGAAATTATCGCGATGCAGGACGCGATCTCGGCGATCGAGAAGTGCCGGAAGCCGGTGATTGCGAGCATCCACGGCGCGTGCATCGGCGGAGGGGTTGATATCGTCACCGCCTGTGATGTAAGGTACTGCACGGCGGAGGCGTATTTCTCGGTGAAGGAGGTTAATTTGGGGATCGCCGCCGATCTTGGGACTCTGCAGAGGCTGCCGGCGATCGTCGGGTACGGGAGGGCGGCGGAATTGGCGTTGACCGGCCGGAACTTTTCGGGCCTGGAGGCTAAGGAATTGGGCCTCGTTTCTAAAACATTCGCTTCCAAGGAGGATCTCGACAATGGAGTGACCAAAATTGCTGAAGGTATTTTTTTTTTTTCTCTTTTTCTTATTTTTGTCATTTCCAATCCTGGTTATGTATTTTCCAATATCTTAAACTATTGTTATTATTTTTTTAATTTTTTATTTCACGTGTCCACATATATTAATAAGATAATAATTTATAGTATATCACAAATTTTAACACTTTTTTTTTTATGAAATCAACGTCGAGAAATTAAAAGGTTAAAGACAAAATTGAAAATTAACTAAAGAATTAGGATCATTTTGTAAATTTAGTGTTAATTAAATTACAAATTTAAACTGACTCTTATGGTTTGGACTTGACCTTAATTTAATCTTTATGATTCTAAAAATTTTAATTTGGTCCTATAGTTTTGTCAAATCTCAATCATTGTCCTCTATAGTTATATCTAAATTTAACCCAATCATAATGACCAAATTGAAAATTTTAAACCAATTTATATGATTAAATCGAAAGTTTTAAAAAACAAATTGGTAACTTATTGAATCTGTAATATATATTTAGGTTAAATTATATATAATCCTGAAGTTTGTGTCTATTTAATATTTAAAATTTAAAAAGTTGTAATTACATTGTTAGTTTTCTTTTTAAATTTTGACTCTTTTCTAATAAGTCTATTTTGTTAAATATGGACGTGAACCATTAGCTGGACCCAAAAAATGGATTTAAATGTATTTTTGTCCACATGTCCCATTAATTTTTCTGGTCACCCATGTTAGCTTGACATTGATTTTAGGTAAAAGGATGGACTTACTCTAACTTTCCTATATAATTTATTTAAGGAGAAATTTAAAATTTAAATATTATTTTAGTCTCTATACCTTCCAAAACGTCTTTATTTGGTCATATTAAAAAAGATGACATAAAAAAGAAGATCAAAATGATTTGAGAAATAAAACAAAAAAAACTAAAATAGGATTTGAACCAAGTAAAAAAGAGAGAATTAATTTTAGAAAAATTGAGAAGAAATTAATGAGTGGTGAAATAGCAGAAATAGCATCAAAATCTCCCCTGGCTGTGGTGGGAACGAAGGCAGTGTTACTAAAGAGCAGGGACCTGAGCGTGGAACAAGCCTTGGATTATGTGGCCACTTGGAACTCAGCAACGCTGTTCTCAGATGATATCAAAGAGGCAGTCTCCGCCCATGTTCATAAACGCAACCCTGTTTTTGCCAAGCTC

mRNA sequence

GAAGGCTACAAATTCGTAAAAATCGTCCGTCAGAATCGAAGCTCCGCCGTGTTCCACCTCCGCCTCCACCGCCCCTCCCACCACAATGCCTTCACCACCGACCTACTCTTCGAATTCCGTCGAGCCCTCTCTTCTCTTGACCAAAACCCCGACGCTCGCGTCATCGTCCTCTCCGGATCCGGCAAAAATTTCTGCGCCGGAATCGATCTCAAATCCCTTGCATCCATTTTCCAGAAATTTCAGTCCGGCGACCGCGGCCGCGCCGGCGAGCAGCTCCGGCGGGAAATTATCGCGATGCAGGACGCGATCTCGGCGATCGAGAAGTGCCGGAAGCCGGTGATTGCGAGCATCCACGGCGCGTGCATCGGCGGAGGGGTTGATATCGTCACCGCCTGTGATGTAAGGTACTGCACGGCGGAGGCGTATTTCTCGGTGAAGGAGGTTAATTTGGGGATCGCCGCCGATCTTGGGACTCTGCAGAGGCTGCCGGCGATCGTCGGGTACGGGAGGGCGGCGGAATTGGCGTTGACCGGCCGGAACTTTTCGGGCCTGGAGGCTAAGGAATTGGGCCTCGTTTCTAAAACATTCGCTTCCAAGGAGGATCTCGACAATGGAGTGACCAAAATTGCTGAAGCAGAAATAGCATCAAAATCTCCCCTGGCTGTGGTGGGAACGAAGGCAGTGTTACTAAAGAGCAGGGACCTGAGCGTGGAACAAGCCTTGGATTATGTGGCCACTTGGAACTCAGCAACGCTGTTCTCAGATGATATCAAAGAGGCAGTCTCCGCCCATGTTCATAAACGCAACCCTGTTTTTGCCAAGCTC

Coding sequence (CDS)

GAAGGCTACAAATTCGTAAAAATCGTCCGTCAGAATCGAAGCTCCGCCGTGTTCCACCTCCGCCTCCACCGCCCCTCCCACCACAATGCCTTCACCACCGACCTACTCTTCGAATTCCGTCGAGCCCTCTCTTCTCTTGACCAAAACCCCGACGCTCGCGTCATCGTCCTCTCCGGATCCGGCAAAAATTTCTGCGCCGGAATCGATCTCAAATCCCTTGCATCCATTTTCCAGAAATTTCAGTCCGGCGACCGCGGCCGCGCCGGCGAGCAGCTCCGGCGGGAAATTATCGCGATGCAGGACGCGATCTCGGCGATCGAGAAGTGCCGGAAGCCGGTGATTGCGAGCATCCACGGCGCGTGCATCGGCGGAGGGGTTGATATCGTCACCGCCTGTGATGTAAGGTACTGCACGGCGGAGGCGTATTTCTCGGTGAAGGAGGTTAATTTGGGGATCGCCGCCGATCTTGGGACTCTGCAGAGGCTGCCGGCGATCGTCGGGTACGGGAGGGCGGCGGAATTGGCGTTGACCGGCCGGAACTTTTCGGGCCTGGAGGCTAAGGAATTGGGCCTCGTTTCTAAAACATTCGCTTCCAAGGAGGATCTCGACAATGGAGTGACCAAAATTGCTGAAGCAGAAATAGCATCAAAATCTCCCCTGGCTGTGGTGGGAACGAAGGCAGTGTTACTAAAGAGCAGGGACCTGAGCGTGGAACAAGCCTTGGATTATGTGGCCACTTGGAACTCAGCAACGCTGTTCTCAGATGATATCAAAGAGGCAGTCTCCGCCCATGTTCATAAACGCAACCCTGTTTTTGCCAAGCTC

Protein sequence

EGYKFVKIVRQNRSSAVFHLRLHRPSHHNAFTTDLLFEFRRALSSLDQNPDARVIVLSGSGKNFCAGIDLKSLASIFQKFQSGDRGRAGEQLRREIIAMQDAISAIEKCRKPVIASIHGACIGGGVDIVTACDVRYCTAEAYFSVKEVNLGIAADLGTLQRLPAIVGYGRAAELALTGRNFSGLEAKELGLVSKTFASKEDLDNGVTKIAEAEIASKSPLAVVGTKAVLLKSRDLSVEQALDYVATWNSATLFSDDIKEAVSAHVHKRNPVFAKL

Homology

[Close]

BLAST of MS001640 vs. NCBI nr
Match: XP_023526792.1 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 424.5 bits (1090), Expect = 7.1e-115
Identity = 218/275 (79.27%), Postives = 238/275 (86.55%), Query Frame = 0


Query: 1   EGYKFVKIVRQNRSSAVFHLRLHRPSHHNAFTTDLLFEFRRALSSLDQNPDARVIVLSGS 60
           E YK +KI RQN SS VFHLRLHRPSH+NA TT+L  E RRALSSLDQNPD RVI+LSGS
Sbjct: 2   EDYKSIKIERQNPSSGVFHLRLHRPSHYNALTTELFAELRRALSSLDQNPDVRVIILSGS 61

Query: 61  GKNFCAGIDLKSLASIFQKFQSGDRGRAGEQLRREIIAMQDAISAIEKCRKPVIASIHGA 120
           GK+FCAGIDLKS AS F+   S +RGRAGE+LRRE+ AMQD+I+AIE+CRKPVIA IHG 
Sbjct: 62  GKHFCAGIDLKSTASNFENLLSSERGRAGERLRREVKAMQDSITAIEECRKPVIACIHGG 121

Query: 121 CIGGGVDIVTACDVRYCTAEAYFSVKEVNLGIAADLGTLQRLPAIVGYGRAAELALTGRN 180
           CIGGGVDI TACD+RYCTAEA FSVKEVNL I ADLGTLQRLP IVG+G+A ELALTGR 
Sbjct: 122 CIGGGVDIATACDLRYCTAEAVFSVKEVNLAITADLGTLQRLPGIVGFGKAVELALTGRE 181

Query: 181 FSGLEAKELGLVSKTFASKEDLDNGVTKIAEAEIASKSPLAVVGTKAVLLKSRDLSVEQA 240
           FSGLEAKELGLVS TFASK +LDNGV KIAE EIASKSPLAVVGTKAVLLKSRDL+VEQ 
Sbjct: 182 FSGLEAKELGLVSTTFASKMELDNGVIKIAE-EIASKSPLAVVGTKAVLLKSRDLTVEQG 241

Query: 241 LDYVATWNSATLFSDDIKEAVSAHVHKRNPVFAKL 276
           LDYVATWNS TL S D+KEA+SA  HKRNP F+KL
Sbjct: 242 LDYVATWNSGTLLSADLKEAISARAHKRNPTFSKL 275

[Close]

BLAST of MS001640 vs. NCBI nr
Match: XP_022956301.1 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucurbita moschata] >KAG7018444.1 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 421.0 bits (1081), Expect = 7.8e-114
Identity = 215/275 (78.18%), Postives = 237/275 (86.18%), Query Frame = 0


Query: 1   EGYKFVKIVRQNRSSAVFHLRLHRPSHHNAFTTDLLFEFRRALSSLDQNPDARVIVLSGS 60
           E YK +KI RQN +S VFHLRLHRPSH+NA TT+L  E RRALSSLDQNPD RVI+LSGS
Sbjct: 2   EDYKSIKIERQNPNSGVFHLRLHRPSHYNALTTELFAELRRALSSLDQNPDVRVIILSGS 61

Query: 61  GKNFCAGIDLKSLASIFQKFQSGDRGRAGEQLRREIIAMQDAISAIEKCRKPVIASIHGA 120
           GK+FCAGIDLKS AS F+   S +RGRAGE+LRRE+ AMQD+I+AIE+CRKPVIA IHG 
Sbjct: 62  GKHFCAGIDLKSTASNFEDLLSSERGRAGERLRREVKAMQDSITAIEECRKPVIACIHGG 121

Query: 121 CIGGGVDIVTACDVRYCTAEAYFSVKEVNLGIAADLGTLQRLPAIVGYGRAAELALTGRN 180
           CIGGGVDI TACD+RYCTAEA FSVKE+NL I ADLGTLQRLP IVG+G+A ELALTGR 
Sbjct: 122 CIGGGVDIATACDLRYCTAEAVFSVKEINLAITADLGTLQRLPGIVGFGKAVELALTGRE 181

Query: 181 FSGLEAKELGLVSKTFASKEDLDNGVTKIAEAEIASKSPLAVVGTKAVLLKSRDLSVEQA 240
           FSG EAKELGLVS TFASK +LDNGV KIAE EIASKSPLAVVGTKAVLLKSRDL+VEQ 
Sbjct: 182 FSGFEAKELGLVSTTFASKMELDNGVIKIAE-EIASKSPLAVVGTKAVLLKSRDLTVEQG 241

Query: 241 LDYVATWNSATLFSDDIKEAVSAHVHKRNPVFAKL 276
           LDYVATWNS TL S D+KEA+SA  HKRNP F+KL
Sbjct: 242 LDYVATWNSGTLLSADLKEAISARAHKRNPTFSKL 275

[Close]

BLAST of MS001640 vs. NCBI nr
Match: XP_022979902.1 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucurbita maxima])

HSP 1 Score: 418.7 bits (1075), Expect = 3.9e-113
Identity = 214/275 (77.82%), Postives = 237/275 (86.18%), Query Frame = 0


Query: 1   EGYKFVKIVRQNRSSAVFHLRLHRPSHHNAFTTDLLFEFRRALSSLDQNPDARVIVLSGS 60
           E YK +KI RQN +S VFHLRLHRPSH+N  TT+L  E RRALSSLDQNPD RVI+LSGS
Sbjct: 2   EDYKSIKIERQNPNSGVFHLRLHRPSHYNTLTTELFGELRRALSSLDQNPDVRVIILSGS 61

Query: 61  GKNFCAGIDLKSLASIFQKFQSGDRGRAGEQLRREIIAMQDAISAIEKCRKPVIASIHGA 120
           GK+FCAGIDLKS AS F+   S +RGRAGE+LRRE+ AMQD+I+AIE+CRKPVIA IHG 
Sbjct: 62  GKHFCAGIDLKSTASNFENLLSSERGRAGERLRREVKAMQDSITAIEECRKPVIACIHGG 121

Query: 121 CIGGGVDIVTACDVRYCTAEAYFSVKEVNLGIAADLGTLQRLPAIVGYGRAAELALTGRN 180
           CIGGGVD+ TACD+RYCTAEA FSVKEVNL I ADLGTLQRLP IVG+G+A ELALTGR 
Sbjct: 122 CIGGGVDMATACDLRYCTAEAVFSVKEVNLAITADLGTLQRLPGIVGFGKAVELALTGRE 181

Query: 181 FSGLEAKELGLVSKTFASKEDLDNGVTKIAEAEIASKSPLAVVGTKAVLLKSRDLSVEQA 240
           FSGLEAKELGLVS TFASK +L+NGV KIAE EIASKSPLAVVGTKAVLLKSRDL+VEQ 
Sbjct: 182 FSGLEAKELGLVSATFASKMELENGVIKIAE-EIASKSPLAVVGTKAVLLKSRDLTVEQG 241

Query: 241 LDYVATWNSATLFSDDIKEAVSAHVHKRNPVFAKL 276
           LDYVATWNS TL S D+KEA+SA  HKRNP F+KL
Sbjct: 242 LDYVATWNSGTLLSADLKEAISARAHKRNPAFSKL 275

[Close]

BLAST of MS001640 vs. NCBI nr
Match: XP_038879593.1 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal isoform X1 [Benincasa hispida])

HSP 1 Score: 414.1 bits (1063), Expect = 9.6e-112
Identity = 212/275 (77.09%), Postives = 240/275 (87.27%), Query Frame = 0


Query: 1   EGYKFVKIVRQNRSSAVFHLRLHRPSHHNAFTTDLLFEFRRALSSLDQNPDARVIVLSGS 60
           EGYK +KI RQN +S VFHLRLHRPSHHNA TT+L  E R+A S LDQNP+  VI+LSGS
Sbjct: 2   EGYKCIKIDRQNPNSGVFHLRLHRPSHHNALTTELFAELRQAFSYLDQNPEVHVIILSGS 61

Query: 61  GKNFCAGIDLKSLASIFQKFQSGDRGRAGEQLRREIIAMQDAISAIEKCRKPVIASIHGA 120
           GK+FCAGIDLKS AS F K QS +RGRAGE+LRREI  MQ++I+AIE+CRKPVIASIHG 
Sbjct: 62  GKHFCAGIDLKSTASNFSKLQSEERGRAGERLRREIKWMQESITAIEECRKPVIASIHGG 121

Query: 121 CIGGGVDIVTACDVRYCTAEAYFSVKEVNLGIAADLGTLQRLPAIVGYGRAAELALTGRN 180
           CIGGGVDIVTACD+RYCTAEA FSV+EV + I ADLGTLQRLP IVGYG+AAELALTGR+
Sbjct: 122 CIGGGVDIVTACDLRYCTAEAVFSVREVRMAITADLGTLQRLPRIVGYGKAAELALTGRD 181

Query: 181 FSGLEAKELGLVSKTFASKEDLDNGVTKIAEAEIASKSPLAVVGTKAVLLKSRDLSVEQA 240
           FSGLEAKELGLVS+TFASK +L++ V KIA+ EI SKSPLAVVGTKAVLLKSRDL+VEQ 
Sbjct: 182 FSGLEAKELGLVSRTFASKLELEDEVLKIAQ-EIGSKSPLAVVGTKAVLLKSRDLNVEQG 241

Query: 241 LDYVATWNSATLFSDDIKEAVSAHVHKRNPVFAKL 276
           LDYVATWNS TL SDD+KEA+SA  +KRNPVF+KL
Sbjct: 242 LDYVATWNSGTLLSDDLKEAISAQANKRNPVFSKL 275

[Close]

BLAST of MS001640 vs. NCBI nr
Match: KAG6582020.1 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 407.5 bits (1046), Expect = 9.0e-110
Identity = 209/267 (78.28%), Postives = 230/267 (86.14%), Query Frame = 0


Query: 1   EGYKFVKIVRQNRSSAVFHLRLHRPSHHNAFTTDLLFEFRRALSSLDQNPDARVIVLSGS 60
           E YK +KI RQN +S VFHLRLHRPSH+NA TT+L  E RRALSSLDQNPD RVI+LSGS
Sbjct: 2   EDYKSIKIERQNPNSGVFHLRLHRPSHYNALTTELFAELRRALSSLDQNPDVRVIILSGS 61

Query: 61  GKNFCAGIDLKSLASIFQKFQSGDRGRAGEQLRREIIAMQDAISAIEKCRKPVIASIHGA 120
           GK+FCAGIDLKS AS F+   S +RGRAGE+LRRE+ AMQD+I+AIE+CRKPVIA IHG 
Sbjct: 62  GKHFCAGIDLKSTASNFEDLLSSERGRAGERLRREVKAMQDSITAIEECRKPVIACIHGG 121

Query: 121 CIGGGVDIVTACDVRYCTAEAYFSVKEVNLGIAADLGTLQRLPAIVGYGRAAELALTGRN 180
           CIGGGVDI TACD+RYCTAEA FSVKE+NL I ADLGTLQRLP IVG+G+A ELALTGR 
Sbjct: 122 CIGGGVDIATACDLRYCTAEAVFSVKEINLAITADLGTLQRLPGIVGFGKAVELALTGRE 181

Query: 181 FSGLEAKELGLVSKTFASKEDLDNGVTKIAEAEIASKSPLAVVGTKAVLLKSRDLSVEQA 240
           FSG EAKELGLVS TFASK +LDNGV KIAE EIASKSPLAVVGTKAVLLKSRDL+VEQ 
Sbjct: 182 FSGFEAKELGLVSTTFASKMELDNGVIKIAE-EIASKSPLAVVGTKAVLLKSRDLTVEQG 241

Query: 241 LDYVATWNSATLFSDDIKEAVSAHVHK 268
           LDYVATWNS TL S D+KEA+SA  HK
Sbjct: 242 LDYVATWNSGTLLSADLKEAISARAHK 267

[Close]

BLAST of MS001640 vs. ExPASy Swiss-Prot
Match: Q9FHR8 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=DCI1 PE=1 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 9.3e-102
Identity = 190/276 (68.84%), Postives = 227/276 (82.25%), Query Frame = 0


Query: 1   EGYKFVKIVRQNRSSAVFHLRLHRPSHHNAFTTDLLFEFRRALSSLDQNPDARVIVLSGS 60
           E YK ++I+R+N  S+VFHL ++RPSH NA + D   EF +ALSSLDQNPD  VI+LSG+
Sbjct: 4   ESYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIILSGA 63

Query: 61  GKNFCAGIDLKSLASIFQKFQSG-DRGRAGEQLRREIIAMQDAISAIEKCRKPVIASIHG 120
           GK+FC+GIDL SL+SI  +  SG DRGR+ EQLRR+I +MQ AI+AIE+CRKPVIA+IHG
Sbjct: 64  GKHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQAAITAIEQCRKPVIAAIHG 123

Query: 121 ACIGGGVDIVTACDVRYCTAEAYFSVKEVNLGIAADLGTLQRLPAIVGYGRAAELALTGR 180
           ACIGGGVD++TACD+RYC+ +A+FS+KEV+L I ADLGTLQRLP+IVGY  A ELALT R
Sbjct: 124 ACIGGGVDLITACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELALTAR 183

Query: 181 NFSGLEAKELGLVSKTFASKEDLDNGVTKIAEAEIASKSPLAVVGTKAVLLKSRDLSVEQ 240
            FSG EAK+LGLVSK F SK +LDNGVT IAE  I  KSPLAV GTKAVLL+SR++SVEQ
Sbjct: 184 RFSGSEAKDLGLVSKVFGSKSELDNGVTTIAEG-IGGKSPLAVTGTKAVLLRSREVSVEQ 243

Query: 241 ALDYVATWNSATLFSDDIKEAVSAHVHKRNPVFAKL 276
            LDYVATWNSA L SDD+ EAVSA + KR P FAKL
Sbjct: 244 GLDYVATWNSAMLISDDLNEAVSAQMMKRKPRFAKL 278

[Close]

BLAST of MS001640 vs. ExPASy Swiss-Prot
Match: O35459 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Mus musculus OX=10090 GN=Ech1 PE=1 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 6.6e-55
Identity = 118/263 (44.87%), Postives = 166/263 (63.12%), Query Frame = 0


Query: 17  VFHLRLHRPSHHNAFTTDLLFEFRRALSSLDQNPDARVIVLSGSGKNFCAGIDLKSLASI 76
           V H++L+RP   NA       E       + ++ D R +V+SG+GK F +GIDL  +AS 
Sbjct: 66  VLHVQLNRPEKRNAMNRAFWRELVECFQKISKDSDCRAVVVSGAGKMFTSGIDLMDMASE 125

Query: 77  FQKFQSGDRGRAGEQLRREIIAMQDAISAIEKCRKPVIASIHGACIGGGVDIVTACDVRY 136
             +    D  R    LR  I   Q   + IEKC KPVIA+IHG CIGGGVD+V+ACD+RY
Sbjct: 126 LMQPSGDDAARIAWYLRDLISKYQKTFTVIEKCPKPVIAAIHGGCIGGGVDLVSACDIRY 185

Query: 137 CTAEAYFSVKEVNLGIAADLGTLQRLPAIVG-YGRAAELALTGRNFSGLEAKELGLVSKT 196
           CT +A+F +KEV++G+AAD+GTLQRLP ++G      EL  + R     EA + GLVS+ 
Sbjct: 186 CTQDAFFQIKEVDMGLAADVGTLQRLPKVIGNQSLVNELTFSARKMMADEALDSGLVSRV 245

Query: 197 FASKEDLDNGVTKIAEAEIASKSPLAVVGTKAVLLKSRDLSVEQALDYVATWNSATLFSD 256
           F  K+ + N    +A A+I+SKSP+AV G+K  L+ SRD SV+++LDY+ATWN + L + 
Sbjct: 246 FQDKDAMLNAAFALA-ADISSKSPVAVQGSKINLIYSRDHSVDESLDYMATWNMSMLQTQ 305

Query: 257 DIKEAVSAHVHKRNP---VFAKL 276
           DI ++V A + KR+     F+KL
Sbjct: 306 DIIKSVQAAMEKRDTKSITFSKL 327

[Close]

BLAST of MS001640 vs. ExPASy Swiss-Prot
Match: Q62651 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Ech1 PE=1 SV=2)

HSP 1 Score: 213.0 bits (541), Expect = 4.3e-54
Identity = 118/263 (44.87%), Postives = 166/263 (63.12%), Query Frame = 0


Query: 17  VFHLRLHRPSHHNAFTTDLLFEFRRALSSLDQNPDARVIVLSGSGKNFCAGIDLKSLASI 76
           V H++L+RP   NA       E       + ++ D R +V+SG+GK F +GIDL  +AS 
Sbjct: 66  VLHVQLNRPEKRNAMNRAFWRELVECFQKISKDSDCRAVVVSGAGKMFTSGIDLMDMASD 125

Query: 77  FQKFQSGDRGRAGEQLRREIIAMQDAISAIEKCRKPVIASIHGACIGGGVDIVTACDVRY 136
             +    D  R    LR  I   Q   + IEKC KPVIA+IHG CIGGGVD+++ACD+RY
Sbjct: 126 ILQPPGDDVARIAWYLRDLISRYQKTFTVIEKCPKPVIAAIHGGCIGGGVDLISACDIRY 185

Query: 137 CTAEAYFSVKEVNLGIAADLGTLQRLPAIVG-YGRAAELALTGRNFSGLEAKELGLVSKT 196
           CT +A+F VKEV++G+AAD+GTLQRLP ++G      EL  T R     EA + GLVS+ 
Sbjct: 186 CTQDAFFQVKEVDVGLAADVGTLQRLPKVIGNRSLVNELTFTARKMMADEALDSGLVSRV 245

Query: 197 FASKEDLDNGVTKIAEAEIASKSPLAVVGTKAVLLKSRDLSVEQALDYVATWNSATLFSD 256
           F  K+ + N    +A A+I+SKSP+AV G+K  L+ SRD SV+++LDY+ATWN + L + 
Sbjct: 246 FPDKDVMLNAAFALA-ADISSKSPVAVQGSKINLIYSRDHSVDESLDYMATWNMSMLQTQ 305

Query: 257 DIKEAVSAHVHKRNP---VFAKL 276
           DI ++V A + K++     F+KL
Sbjct: 306 DIIKSVQAAMEKKDSKSITFSKL 327

[Close]

BLAST of MS001640 vs. ExPASy Swiss-Prot
Match: Q5RFG0 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Pongo abelii OX=9601 GN=ECH1 PE=2 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 9.5e-54
Identity = 118/263 (44.87%), Postives = 165/263 (62.74%), Query Frame = 0


Query: 17  VFHLRLHRPSHHNAFTTDLLFEFRRALSSLDQNPDARVIVLSGSGKNFCAGIDLKSLASI 76
           V H++L+RP+  NA       E     + + ++ D R +V+SG+GK F AG+DL  +AS 
Sbjct: 67  VLHVQLNRPNKRNAMNKVFWREMVECFNKISRDADCRAVVISGAGKMFTAGVDLMDMASD 126

Query: 77  FQKFQSGDRGRAGEQLRREIIAMQDAISAIEKCRKPVIASIHGACIGGGVDIVTACDVRY 136
             + +  D  R    LR  I   Q+  + IEKC KPVIA++HG CIGGGVD+VTACD+RY
Sbjct: 127 ILQPKGDDVARISWYLRDIITRYQETFNVIEKCPKPVIAAVHGGCIGGGVDLVTACDIRY 186

Query: 137 CTAEAYFSVKEVNLGIAADLGTLQRLPAIVG-YGRAAELALTGRNFSGLEAKELGLVSKT 196
           C  +A+F VKEV++G+AAD+GTLQRLP ++G      ELA T R     EA   GLVS+ 
Sbjct: 187 CAQDAFFQVKEVDVGLAADVGTLQRLPKVIGNQSLVNELAFTARKMMADEALGSGLVSRV 246

Query: 197 FASKEDLDNGVTKIAEAEIASKSPLAVVGTKAVLLKSRDLSVEQALDYVATWNSATLFSD 256
           F  KE + +    +A AEI+SKSP+AV  TK  LL SRD SV ++L+YVA+WN + L + 
Sbjct: 247 FPDKEVMLDAALALA-AEISSKSPVAVQSTKVNLLYSRDHSVAESLNYVASWNMSMLQTQ 306

Query: 257 DIKEAVSAHVHK---RNPVFAKL 276
           D+ ++V A       ++  F+KL
Sbjct: 307 DLMKSVQAATENKELKSVTFSKL 328

[Close]

BLAST of MS001640 vs. ExPASy Swiss-Prot
Match: Q13011 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Homo sapiens OX=9606 GN=ECH1 PE=1 SV=2)

HSP 1 Score: 209.9 bits (533), Expect = 3.6e-53
Identity = 115/248 (46.37%), Postives = 159/248 (64.11%), Query Frame = 0


Query: 17  VFHLRLHRPSHHNAFTTDLLFEFRRALSSLDQNPDARVIVLSGSGKNFCAGIDLKSLASI 76
           V H++L+RP+  NA       E     + + ++ D R +V+SG+GK F AGIDL  +AS 
Sbjct: 67  VLHVQLNRPNKRNAMNKVFWREMVECFNKISRDADCRAVVISGAGKMFTAGIDLMDMASD 126

Query: 77  FQKFQSGDRGRAGEQLRREIIAMQDAISAIEKCRKPVIASIHGACIGGGVDIVTACDVRY 136
             + +  D  R    LR  I   Q+  + IE+C KPVIA++HG CIGGGVD+VTACD+RY
Sbjct: 127 ILQPKGDDVARISWYLRDIITRYQETFNVIERCPKPVIAAVHGGCIGGGVDLVTACDIRY 186

Query: 137 CTAEAYFSVKEVNLGIAADLGTLQRLPAIVG-YGRAAELALTGRNFSGLEAKELGLVSKT 196
           C  +A+F VKEV++G+AAD+GTLQRLP ++G      ELA T R     EA   GLVS+ 
Sbjct: 187 CAQDAFFQVKEVDVGLAADVGTLQRLPKVIGNQSLVNELAFTARKMMADEALGSGLVSRV 246

Query: 197 FASKEDLDNGVTKIAEAEIASKSPLAVVGTKAVLLKSRDLSVEQALDYVATWNSATLFSD 256
           F  KE + +    +A AEI+SKSP+AV  TK  LL SRD SV ++L+YVA+WN + L + 
Sbjct: 247 FPDKEVMLDAALALA-AEISSKSPVAVQSTKVNLLYSRDHSVAESLNYVASWNMSMLQTQ 306

Query: 257 DIKEAVSA 264
           D+ ++V A
Sbjct: 307 DLVKSVQA 313

[Close]

BLAST of MS001640 vs. ExPASy TrEMBL
Match: A0A6J1GW60 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111458038 PE=3 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 3.8e-114
Identity = 215/275 (78.18%), Postives = 237/275 (86.18%), Query Frame = 0


Query: 1   EGYKFVKIVRQNRSSAVFHLRLHRPSHHNAFTTDLLFEFRRALSSLDQNPDARVIVLSGS 60
           E YK +KI RQN +S VFHLRLHRPSH+NA TT+L  E RRALSSLDQNPD RVI+LSGS
Sbjct: 2   EDYKSIKIERQNPNSGVFHLRLHRPSHYNALTTELFAELRRALSSLDQNPDVRVIILSGS 61

Query: 61  GKNFCAGIDLKSLASIFQKFQSGDRGRAGEQLRREIIAMQDAISAIEKCRKPVIASIHGA 120
           GK+FCAGIDLKS AS F+   S +RGRAGE+LRRE+ AMQD+I+AIE+CRKPVIA IHG 
Sbjct: 62  GKHFCAGIDLKSTASNFEDLLSSERGRAGERLRREVKAMQDSITAIEECRKPVIACIHGG 121

Query: 121 CIGGGVDIVTACDVRYCTAEAYFSVKEVNLGIAADLGTLQRLPAIVGYGRAAELALTGRN 180
           CIGGGVDI TACD+RYCTAEA FSVKE+NL I ADLGTLQRLP IVG+G+A ELALTGR 
Sbjct: 122 CIGGGVDIATACDLRYCTAEAVFSVKEINLAITADLGTLQRLPGIVGFGKAVELALTGRE 181

Query: 181 FSGLEAKELGLVSKTFASKEDLDNGVTKIAEAEIASKSPLAVVGTKAVLLKSRDLSVEQA 240
           FSG EAKELGLVS TFASK +LDNGV KIAE EIASKSPLAVVGTKAVLLKSRDL+VEQ 
Sbjct: 182 FSGFEAKELGLVSTTFASKMELDNGVIKIAE-EIASKSPLAVVGTKAVLLKSRDLTVEQG 241

Query: 241 LDYVATWNSATLFSDDIKEAVSAHVHKRNPVFAKL 276
           LDYVATWNS TL S D+KEA+SA  HKRNP F+KL
Sbjct: 242 LDYVATWNSGTLLSADLKEAISARAHKRNPTFSKL 275

[Close]

BLAST of MS001640 vs. ExPASy TrEMBL
Match: A0A6J1IPZ5 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111479454 PE=3 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 1.9e-113
Identity = 214/275 (77.82%), Postives = 237/275 (86.18%), Query Frame = 0


Query: 1   EGYKFVKIVRQNRSSAVFHLRLHRPSHHNAFTTDLLFEFRRALSSLDQNPDARVIVLSGS 60
           E YK +KI RQN +S VFHLRLHRPSH+N  TT+L  E RRALSSLDQNPD RVI+LSGS
Sbjct: 2   EDYKSIKIERQNPNSGVFHLRLHRPSHYNTLTTELFGELRRALSSLDQNPDVRVIILSGS 61

Query: 61  GKNFCAGIDLKSLASIFQKFQSGDRGRAGEQLRREIIAMQDAISAIEKCRKPVIASIHGA 120
           GK+FCAGIDLKS AS F+   S +RGRAGE+LRRE+ AMQD+I+AIE+CRKPVIA IHG 
Sbjct: 62  GKHFCAGIDLKSTASNFENLLSSERGRAGERLRREVKAMQDSITAIEECRKPVIACIHGG 121

Query: 121 CIGGGVDIVTACDVRYCTAEAYFSVKEVNLGIAADLGTLQRLPAIVGYGRAAELALTGRN 180
           CIGGGVD+ TACD+RYCTAEA FSVKEVNL I ADLGTLQRLP IVG+G+A ELALTGR 
Sbjct: 122 CIGGGVDMATACDLRYCTAEAVFSVKEVNLAITADLGTLQRLPGIVGFGKAVELALTGRE 181

Query: 181 FSGLEAKELGLVSKTFASKEDLDNGVTKIAEAEIASKSPLAVVGTKAVLLKSRDLSVEQA 240
           FSGLEAKELGLVS TFASK +L+NGV KIAE EIASKSPLAVVGTKAVLLKSRDL+VEQ 
Sbjct: 182 FSGLEAKELGLVSATFASKMELENGVIKIAE-EIASKSPLAVVGTKAVLLKSRDLTVEQG 241

Query: 241 LDYVATWNSATLFSDDIKEAVSAHVHKRNPVFAKL 276
           LDYVATWNS TL S D+KEA+SA  HKRNP F+KL
Sbjct: 242 LDYVATWNSGTLLSADLKEAISARAHKRNPAFSKL 275

[Close]

BLAST of MS001640 vs. ExPASy TrEMBL
Match: A0A5D3D1B6 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001700 PE=3 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 1.1e-108
Identity = 206/275 (74.91%), Postives = 236/275 (85.82%), Query Frame = 0


Query: 1   EGYKFVKIVRQNRSSAVFHLRLHRPSHHNAFTTDLLFEFRRALSSLDQNPDARVIVLSGS 60
           EGY+ +KI RQ+ +S VFHLRLHRPSH+NA TT L  E R+A S LDQNPD  VI+LSGS
Sbjct: 2   EGYECIKIERQSPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSRLDQNPDVHVIILSGS 61

Query: 61  GKNFCAGIDLKSLASIFQKFQSGDRGRAGEQLRREIIAMQDAISAIEKCRKPVIASIHGA 120
           GK+FCAGIDLKS AS F+K  S +RGRAGE++RREI  MQ++I+AIE+CRKPVIASIHG 
Sbjct: 62  GKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGG 121

Query: 121 CIGGGVDIVTACDVRYCTAEAYFSVKEVNLGIAADLGTLQRLPAIVGYGRAAELALTGRN 180
           CIGGG+DIVTACD+RYCTAEA FSV+EV L I ADLGTLQRLP IVGYG+AAELALTGR+
Sbjct: 122 CIGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGRD 181

Query: 181 FSGLEAKELGLVSKTFASKEDLDNGVTKIAEAEIASKSPLAVVGTKAVLLKSRDLSVEQA 240
           FSGLEAKELGLVS+TF S  +L +GV KIAE EIAS+SPLAVVGTKAVLLKSRDL+VEQ 
Sbjct: 182 FSGLEAKELGLVSRTFDSMSELQDGVLKIAE-EIASRSPLAVVGTKAVLLKSRDLNVEQG 241

Query: 241 LDYVATWNSATLFSDDIKEAVSAHVHKRNPVFAKL 276
           LDYVATWNS TL SDD+KEA+SA  +KR P F+KL
Sbjct: 242 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275

[Close]

BLAST of MS001640 vs. ExPASy TrEMBL
Match: A0A1S3BXS5 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103494575 PE=3 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 1.1e-108
Identity = 206/275 (74.91%), Postives = 236/275 (85.82%), Query Frame = 0


Query: 1   EGYKFVKIVRQNRSSAVFHLRLHRPSHHNAFTTDLLFEFRRALSSLDQNPDARVIVLSGS 60
           EGY+ +KI RQ+ +S VFHLRLHRPSH+NA TT L  E R+A S LDQNPD  VI+LSGS
Sbjct: 2   EGYECIKIERQSPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSRLDQNPDVHVIILSGS 61

Query: 61  GKNFCAGIDLKSLASIFQKFQSGDRGRAGEQLRREIIAMQDAISAIEKCRKPVIASIHGA 120
           GK+FCAGIDLKS AS F+K  S +RGRAGE++RREI  MQ++I+AIE+CRKPVIASIHG 
Sbjct: 62  GKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGG 121

Query: 121 CIGGGVDIVTACDVRYCTAEAYFSVKEVNLGIAADLGTLQRLPAIVGYGRAAELALTGRN 180
           CIGGG+DIVTACD+RYCTAEA FSV+EV L I ADLGTLQRLP IVGYG+AAELALTGR+
Sbjct: 122 CIGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGRD 181

Query: 181 FSGLEAKELGLVSKTFASKEDLDNGVTKIAEAEIASKSPLAVVGTKAVLLKSRDLSVEQA 240
           FSGLEAKELGLVS+TF S  +L +GV KIAE EIAS+SPLAVVGTKAVLLKSRDL+VEQ 
Sbjct: 182 FSGLEAKELGLVSRTFDSMSELQDGVLKIAE-EIASRSPLAVVGTKAVLLKSRDLNVEQG 241

Query: 241 LDYVATWNSATLFSDDIKEAVSAHVHKRNPVFAKL 276
           LDYVATWNS TL SDD+KEA+SA  +KR P F+KL
Sbjct: 242 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275

[Close]

BLAST of MS001640 vs. ExPASy TrEMBL
Match: A0A5A7TMZ4 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003090 PE=3 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 2.4e-108
Identity = 206/275 (74.91%), Postives = 235/275 (85.45%), Query Frame = 0


Query: 1   EGYKFVKIVRQNRSSAVFHLRLHRPSHHNAFTTDLLFEFRRALSSLDQNPDARVIVLSGS 60
           EGY+ +KI RQ+ +S VFHLRLHRPSH+NA TT L  E R+A S LDQNPD  VI+LSGS
Sbjct: 2   EGYECIKIERQSPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSRLDQNPDVHVIILSGS 61

Query: 61  GKNFCAGIDLKSLASIFQKFQSGDRGRAGEQLRREIIAMQDAISAIEKCRKPVIASIHGA 120
           GK+FCAGIDLKS AS F+K  S +RGRAGE++RREI  MQ++I+AIE+CRKPVIASIHG 
Sbjct: 62  GKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGG 121

Query: 121 CIGGGVDIVTACDVRYCTAEAYFSVKEVNLGIAADLGTLQRLPAIVGYGRAAELALTGRN 180
           CIGGG+DIVTACD+RYCTAEA FSV+EV L I ADLGTLQRLP IVGYG+AAELALTGR+
Sbjct: 122 CIGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGRD 181

Query: 181 FSGLEAKELGLVSKTFASKEDLDNGVTKIAEAEIASKSPLAVVGTKAVLLKSRDLSVEQA 240
           FSGLEAKELGLVS+TF S  +L +GV KIAE EIAS+SPLAVVGTKAVLLKSRDL VEQ 
Sbjct: 182 FSGLEAKELGLVSRTFDSMSELQDGVLKIAE-EIASRSPLAVVGTKAVLLKSRDLYVEQG 241

Query: 241 LDYVATWNSATLFSDDIKEAVSAHVHKRNPVFAKL 276
           LDYVATWNS TL SDD+KEA+SA  +KR P F+KL
Sbjct: 242 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275

[Close]

BLAST of MS001640 vs. TAIR 10
Match: AT5G43280.1 (delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 )

HSP 1 Score: 371.3 bits (952), Expect = 6.6e-103
Identity = 190/276 (68.84%), Postives = 227/276 (82.25%), Query Frame = 0


Query: 1   EGYKFVKIVRQNRSSAVFHLRLHRPSHHNAFTTDLLFEFRRALSSLDQNPDARVIVLSGS 60
           E YK ++I+R+N  S+VFHL ++RPSH NA + D   EF +ALSSLDQNPD  VI+LSG+
Sbjct: 4   ESYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIILSGA 63

Query: 61  GKNFCAGIDLKSLASIFQKFQSG-DRGRAGEQLRREIIAMQDAISAIEKCRKPVIASIHG 120
           GK+FC+GIDL SL+SI  +  SG DRGR+ EQLRR+I +MQ AI+AIE+CRKPVIA+IHG
Sbjct: 64  GKHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQAAITAIEQCRKPVIAAIHG 123

Query: 121 ACIGGGVDIVTACDVRYCTAEAYFSVKEVNLGIAADLGTLQRLPAIVGYGRAAELALTGR 180
           ACIGGGVD++TACD+RYC+ +A+FS+KEV+L I ADLGTLQRLP+IVGY  A ELALT R
Sbjct: 124 ACIGGGVDLITACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELALTAR 183

Query: 181 NFSGLEAKELGLVSKTFASKEDLDNGVTKIAEAEIASKSPLAVVGTKAVLLKSRDLSVEQ 240
            FSG EAK+LGLVSK F SK +LDNGVT IAE  I  KSPLAV GTKAVLL+SR++SVEQ
Sbjct: 184 RFSGSEAKDLGLVSKVFGSKSELDNGVTTIAEG-IGGKSPLAVTGTKAVLLRSREVSVEQ 243

Query: 241 ALDYVATWNSATLFSDDIKEAVSAHVHKRNPVFAKL 276
            LDYVATWNSA L SDD+ EAVSA + KR P FAKL
Sbjct: 244 GLDYVATWNSAMLISDDLNEAVSAQMMKRKPRFAKL 278

[Close]

BLAST of MS001640 vs. TAIR 10
Match: AT5G43280.2 (delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 )

HSP 1 Score: 290.0 bits (741), Expect = 1.9e-78
Identity = 144/212 (67.92%), Postives = 176/212 (83.02%), Query Frame = 0


Query: 1   EGYKFVKIVRQNRSSAVFHLRLHRPSHHNAFTTDLLFEFRRALSSLDQNPDARVIVLSGS 60
           E YK ++I+R+N  S+VFHL ++RPSH NA + D   EF +ALSSLDQNPD  VI+LSG+
Sbjct: 4   ESYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIILSGA 63

Query: 61  GKNFCAGIDLKSLASIFQKFQSG-DRGRAGEQLRREIIAMQDAISAIEKCRKPVIASIHG 120
           GK+FC+GIDL SL+SI  +  SG DRGR+ EQLRR+I +MQ AI+AIE+CRKPVIA+IHG
Sbjct: 64  GKHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQAAITAIEQCRKPVIAAIHG 123

Query: 121 ACIGGGVDIVTACDVRYCTAEAYFSVKEVNLGIAADLGTLQRLPAIVGYGRAAELALTGR 180
           ACIGGGVD++TACD+RYC+ +A+FS+KEV+L I ADLGTLQRLP+IVGY  A ELALT R
Sbjct: 124 ACIGGGVDLITACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELALTAR 183

Query: 181 NFSGLEAKELGLVSKTFASKEDLDNGVTKIAE 212
            FSG EAK+LGLVSK F SK +LDNGVT IAE
Sbjct: 184 RFSGSEAKDLGLVSKVFGSKSELDNGVTTIAE 215

[Close]

BLAST of MS001640 vs. TAIR 10
Match: AT4G16800.1 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 110.9 bits (276), Expect = 1.6e-24
Identity = 80/271 (29.52%), Postives = 130/271 (47.97%), Query Frame = 0


Query: 4   KFVKIVRQNRS-SAVFHLRLHRPSHHNAFTTDLLFEFRRALSSLDQNPDARVIVLSGSGK 63
           +FVK+ R + S S +  + L RP   NA   ++L   + A  S+ Q+  ARV+++     
Sbjct: 41  EFVKLNRLSGSDSGIIEVNLDRPVTKNAINKEMLKSLQNAFESIHQDNSARVVMIRSLVP 100

Query: 64  N-FCAGIDLKSLASIFQKFQSGDRGRAGEQLRREIIAMQDAISAIEKCRKPVIASIHGAC 123
             FCAG DLK             R  +  ++   + +++   S IE    P IA+I GA 
Sbjct: 101 GVFCAGADLKE-----------RRTMSPSEVHTYVNSLRYMFSFIEALSIPTIAAIEGAA 160

Query: 124 IGGGVDIVTACDVRYCTAEAYFSVKEVNLGIAADLGTLQRLPAIVGYGRAAELALTGRNF 183
           +GGG+++  ACD+R C   A F + E  L I    G  QRL  +VG   + EL  TGR  
Sbjct: 161 LGGGLEMALACDLRICGENAVFGLPETGLAIIPGAGGTQRLSRLVGRSVSKELIFTGRKI 220

Query: 184 SGLEAKELGLVSKTFASKEDLDNGVTKIAEAEIASKSPLAVVGTKAVLLKSRDLSVEQAL 243
             +EA   GLV+    + E  +  + ++A+ +I  K PLA+   K  + +  + ++   L
Sbjct: 221 DAIEAANKGLVNICVTAGEAHEKAI-EMAQ-QINEKGPLAIKMAKKAIDEGIETNMASGL 280

Query: 244 DYVATWNSATLFSDDIKEAVSAHVHKRNPVF 273
           +         L + D  E ++A   KR P++
Sbjct: 281 EVEEMCYQKLLNTQDRLEGLAAFAEKRKPLY 298

[Close]

BLAST of MS001640 vs. TAIR 10
Match: AT4G16210.1 (enoyl-CoA hydratase/isomerase A )

HSP 1 Score: 93.2 bits (230), Expect = 3.5e-19
Identity = 63/231 (27.27%), Postives = 113/231 (48.92%), Query Frame = 0


Query: 11  QNRSSAVFHLRLHRPSHHNAFTTDLLFEFRRALSSLDQNPDARVIVLSGSGKNFCAGIDL 70
           +  S  +  + ++RP   N+ T  ++ +  +A   +D +   +V++ +GSG++FC+G+DL
Sbjct: 13  KKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFTGSGRSFCSGVDL 72

Query: 71  KSLASIFQKFQSGDRGRAGEQLRREIIAMQDAISAIEKCRKPVIASIHGACIGGGVDIVT 130
            +  S+F+          G+    E     D +  +E+ RKP+I +I+G  I  G ++  
Sbjct: 73  TAAESVFK----------GDVKDPE----TDPVVQMERLRKPIIGAINGFAITAGFELAL 132

Query: 131 ACDVRYCTAEAYFSVKEVNLGIAADLGTLQRLPAIVGYGRAAELALTGRNFSGLEAKELG 190
           ACD+   +  A F       GI    G  Q+L  I+G  +A E++LT    +   A +LG
Sbjct: 133 ACDILVASRGAKFMDTHARFGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAGKLG 192

Query: 191 LVSKTFASKEDLDNGVTKIAEAEIASKSPLAVVGTKAVLLKSRDLSVEQAL 242
            V+      E L     +IAEA I ++  + V+  K+V+     L +  AL
Sbjct: 193 FVNHVVEEGEALKK-AREIAEAIIKNEQGM-VLRIKSVINDGLKLDLGHAL 227

[Close]

BLAST of MS001640 vs. TAIR 10
Match: AT4G29010.1 (Enoyl-CoA hydratase/isomerase family )

HSP 1 Score: 85.1 bits (209), Expect = 9.5e-17
Identity = 44/164 (26.83%), Postives = 83/164 (50.61%), Query Frame = 0


Query: 29  NAFTTDLLFEFRRALSSLDQNPDARVIVLSGSGKNFCAGIDLKSLASIFQKFQSGDRGRA 88
           N+  + ++   +      +Q  D + IVL G+   F  G D+    ++FQ+       + 
Sbjct: 27  NSLASPIISGLKEKFRDANQRNDVKAIVLIGNNGRFSGGFDI----NVFQQVH-----KT 86

Query: 89  GEQLRREIIAMQDAISAIEKCRKPVIASIHGACIGGGVDIVTACDVRYCTAEAYFSVKEV 148
           G+      ++++   + +E  RKPV+A++ G  +GGG+++  AC  R    +A   + E+
Sbjct: 87  GDLSLMPEVSVELVCNLMEDSRKPVVAAVEGLALGGGLELAMACHARVAAPKAQLGLPEL 146

Query: 149 NLGIAADLGTLQRLPAIVGYGRAAELALTGRNFSGLEAKELGLV 193
            LG+    G  QRLP +VG  +A ++ L  ++ S  E  +LGL+
Sbjct: 147 TLGVIPGFGGTQRLPRLVGLAKATDMILLSKSISSEEGHKLGLI 181

The following BLAST results are available for this feature:

BLAST of MS001640 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023526792.1	7.1e-115	79.27	delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucurbita pepo subsp. ...	[more]
XP_022956301.1	7.8e-114	78.18	delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucurbita moschata] >K...	[more]
XP_022979902.1	3.9e-113	77.82	delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucurbita maxima]	[more]
XP_038879593.1	9.6e-112	77.09	delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal isoform X1 [Benincasa h...	[more]
KAG6582020.1	9.0e-110	78.28	Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal, partial [Cucurbita arg...	[more]

BLAST of MS001640 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FHR8	9.3e-102	68.84	Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Arabidopsis thaliana...	[more]
O35459	6.6e-55	44.87	Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Mus musculus OX=10...	[more]
Q62651	4.3e-54	44.87	Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Rattus norvegicus ...	[more]
Q5RFG0	9.5e-54	44.87	Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Pongo abelii OX=96...	[more]
Q13011	3.6e-53	46.37	Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Homo sapiens OX=96...	[more]

BLAST of MS001640 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GW60	3.8e-114	78.18	delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucurbita moschata O...	[more]
A0A6J1IPZ5	1.9e-113	77.82	delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucurbita maxima OX=...	[more]
A0A5D3D1B6	1.1e-108	74.91	Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucumis melo var. ma...	[more]
A0A1S3BXS5	1.1e-108	74.91	delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucumis melo OX=3656...	[more]
A0A5A7TMZ4	2.4e-108	74.91	Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucumis melo var. ma...	[more]

BLAST of MS001640 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G43280.1	6.6e-103	68.84	delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1	[more]
AT5G43280.2	1.9e-78	67.92	delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1	[more]
AT4G16800.1	1.6e-24	29.52	ATP-dependent caseinolytic (Clp) protease/crotonase family protein	[more]
AT4G16210.1	3.5e-19	27.27	enoyl-CoA hydratase/isomerase A	[more]
AT4G29010.1	9.5e-17	26.83	Enoyl-CoA hydratase/isomerase family	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001753	Enoyl-CoA hydratase/isomerase	PFAM	PF00378	ECH_1	coord: 14..273 e-value: 4.1E-50 score: 170.4
IPR014748	Enoyl-CoA hydratase, C-terminal	GENE3D	1.10.12.10		coord: 218..275 e-value: 1.8E-23 score: 84.5
None	No IPR available	GENE3D	3.90.226.10		coord: 3..217 e-value: 2.5E-63 score: 215.4
None	No IPR available	CDD	cd06558	crotonase-like	coord: 9..211 e-value: 6.65398E-68 score: 207.413
IPR045002	Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase Ech1-like	PANTHER	PTHR43149	ENOYL-COA HYDRATASE	coord: 2..275
IPR018376	Enoyl-CoA hydratase/isomerase, conserved site	PROSITE	PS00166	ENOYL_COA_HYDRATASE	coord: 114..134
IPR029045	ClpP/crotonase-like domain superfamily	SUPERFAMILY	52096	ClpP/crotonase	coord: 7..273

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS001640.1	MS001640.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003824	catalytic activity