MS001474 (gene) Bitter gourd (TR) v1

BLAST of MS001474 vs. NCBI nr
Match: XP_022132108.1 (calcium-transporting ATPase 3, endoplasmic reticulum-type [Momordica charantia])

HSP 1 Score: 1727.2 bits (4472), Expect = 0.0e+00
Identity = 900/900 (100.00%), Postives = 900/900 (100.00%), Query Frame = 0

Query: 1   APFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAFLEPSVILLILAANAAVGVI 60
           APFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAFLEPSVILLILAANAAVGVI
Sbjct: 47  APFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAFLEPSVILLILAANAAVGVI 106

Query: 61  TETNAEKALEELRAYQADVATVMRNGCFSILPATELVPGDIVEVVVGCKIPADMRMIEML 120
           TETNAEKALEELRAYQADVATVMRNGCFSILPATELVPGDIVEVVVGCKIPADMRMIEML
Sbjct: 107 TETNAEKALEELRAYQADVATVMRNGCFSILPATELVPGDIVEVVVGCKIPADMRMIEML 166

Query: 121 SNQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVGAN 180
           SNQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVGAN
Sbjct: 167 SNQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVGAN 226

Query: 181 TAMGNIRDSILQTDDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGVVS 240
           TAMGNIRDSILQTDDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGVVS
Sbjct: 227 TAMGNIRDSILQTDDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGVVS 286

Query: 241 GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS 300
           GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS
Sbjct: 287 GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS 346

Query: 301 DKTGTLTTNMMSVSKICVVHSVLHGPQLSEYSVSGTTYAPDGIIFDSSGVQLEFPAQLPC 360
           DKTGTLTTNMMSVSKICVVHSVLHGPQLSEYSVSGTTYAPDGIIFDSSGVQLEFPAQLPC
Sbjct: 347 DKTGTLTTNMMSVSKICVVHSVLHGPQLSEYSVSGTTYAPDGIIFDSSGVQLEFPAQLPC 406

Query: 361 ILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSK 420
           ILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSK
Sbjct: 407 ILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSK 466

Query: 421 HERASYCNHHWESQFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSILC 480
           HERASYCNHHWESQFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSILC
Sbjct: 467 HERASYCNHHWESQFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSILC 526

Query: 481 NEDGSTSVLTASIRTELEARFQSFAGNEMLRCLAIAFKLIPASQQSLSFGDEQDLTFIGL 540
           NEDGSTSVLTASIRTELEARFQSFAGNEMLRCLAIAFKLIPASQQSLSFGDEQDLTFIGL
Sbjct: 527 NEDGSTSVLTASIRTELEARFQSFAGNEMLRCLAIAFKLIPASQQSLSFGDEQDLTFIGL 586

Query: 541 VGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLTDHSFTAS 600
           VGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLTDHSFTAS
Sbjct: 587 VGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLTDHSFTAS 646

Query: 601 EFEELPAMQQTIALQRMALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADI 660
           EFEELPAMQQTIALQRMALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADI
Sbjct: 647 EFEELPAMQQTIALQRMALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADI 706

Query: 661 GIAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF 720
           GIAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF
Sbjct: 707 GIAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF 766

Query: 721 VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFR 780
           VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFR
Sbjct: 767 VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFR 826

Query: 781 YLVIGAYVGLATIAGFIWWFIYSDSGPKLTYNELMNFDTCSTRETTYPCSIFEDRHPSTV 840
           YLVIGAYVGLATIAGFIWWFIYSDSGPKLTYNELMNFDTCSTRETTYPCSIFEDRHPSTV
Sbjct: 827 YLVIGAYVGLATIAGFIWWFIYSDSGPKLTYNELMNFDTCSTRETTYPCSIFEDRHPSTV 886

Query: 841 SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIFLTMILHMLIMYVRPLAVLFSV 900
           SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIFLTMILHMLIMYVRPLAVLFSV
Sbjct: 887 SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIFLTMILHMLIMYVRPLAVLFSV 946

BLAST of MS001474 vs. NCBI nr
Match: XP_038886243.1 (calcium-transporting ATPase 3, endoplasmic reticulum-type isoform X1 [Benincasa hispida])

HSP 1 Score: 1674.4 bits (4335), Expect = 0.0e+00
Identity = 867/900 (96.33%), Postives = 883/900 (98.11%), Query Frame = 0

Query: 1   APFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAFLEPSVILLILAANAAVGVI 60
           APFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAF+EPSVIL+ILAANAAVGVI
Sbjct: 47  APFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAFMEPSVILMILAANAAVGVI 106

Query: 61  TETNAEKALEELRAYQADVATVMRNGCFSILPATELVPGDIVEVVVGCKIPADMRMIEML 120
           TETNAEKAL ELRAYQA++ATVMRNGCFSILPATELVPGDIVE+ VGCKIPADMRMIEML
Sbjct: 107 TETNAEKALVELRAYQAEIATVMRNGCFSILPATELVPGDIVEIAVGCKIPADMRMIEML 166

Query: 121 SNQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVGAN 180
           +NQ+RVDQAILTGESCSVEKELESTRA NAVYQDKTNILFSGTVVVAGRARAIVVGVGAN
Sbjct: 167 TNQLRVDQAILTGESCSVEKELESTRAANAVYQDKTNILFSGTVVVAGRARAIVVGVGAN 226

Query: 181 TAMGNIRDSILQTDDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGVVS 240
           TAMGNIRDSILQTDDE TPLKKKLDEFGTFLAKVIAGIC LVWIVNIGHFRDPSHGGV+S
Sbjct: 227 TAMGNIRDSILQTDDEVTPLKKKLDEFGTFLAKVIAGICVLVWIVNIGHFRDPSHGGVLS 286

Query: 241 GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS 300
           GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS
Sbjct: 287 GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS 346

Query: 301 DKTGTLTTNMMSVSKICVVHSVLHGPQLSEYSVSGTTYAPDGIIFDSSGVQLEFPAQLPC 360
           DKTGTLTTNMMSVSKICVVHSVLHGPQLSEY+VSGTTYAPDGIIFDSSGVQLEFPAQLPC
Sbjct: 347 DKTGTLTTNMMSVSKICVVHSVLHGPQLSEYNVSGTTYAPDGIIFDSSGVQLEFPAQLPC 406

Query: 361 ILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSK 420
           ILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSK
Sbjct: 407 ILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSK 466

Query: 421 HERASYCNHHWESQFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSILC 480
           HERASYCNHHWESQFKKIS LEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSILC
Sbjct: 467 HERASYCNHHWESQFKKISALEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSILC 526

Query: 481 NEDGSTSVLTASIRTELEARFQSFAGNEMLRCLAIAFKLIPASQQSLSFGDEQDLTFIGL 540
           NEDGSTSVLT+SIRTELEARFQS AGNEMLRCLAIAFKL+P SQQSL+  DEQDLTFIGL
Sbjct: 527 NEDGSTSVLTSSIRTELEARFQSLAGNEMLRCLAIAFKLLPLSQQSLTVNDEQDLTFIGL 586

Query: 541 VGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLTDHSFTAS 600
           VGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLT HS+TAS
Sbjct: 587 VGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLTGHSYTAS 646

Query: 601 EFEELPAMQQTIALQRMALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADI 660
           EFEELPAMQQT ALQRMALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADI
Sbjct: 647 EFEELPAMQQTTALQRMALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADI 706

Query: 661 GIAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF 720
           GIAMGSGTAVAKSASDMVLADDNFA+IVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF
Sbjct: 707 GIAMGSGTAVAKSASDMVLADDNFATIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF 766

Query: 721 VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFR 780
           VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFR
Sbjct: 767 VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFR 826

Query: 781 YLVIGAYVGLATIAGFIWWFIYSDSGPKLTYNELMNFDTCSTRETTYPCSIFEDRHPSTV 840
           YLVIGAYVGLATIAGFIWWFIYSDSGPKL Y ELMNFDTCSTRET Y C+IFEDRHPSTV
Sbjct: 827 YLVIGAYVGLATIAGFIWWFIYSDSGPKLNYTELMNFDTCSTRETAYSCNIFEDRHPSTV 886

Query: 841 SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIFLTMILHMLIMYVRPLAVLFSV 900
           SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASI LTMILHMLIMYV+PLAVLFSV
Sbjct: 887 SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIVLTMILHMLIMYVQPLAVLFSV 946

BLAST of MS001474 vs. NCBI nr
Match: XP_023002575.1 (calcium-transporting ATPase 3, endoplasmic reticulum-type [Cucurbita maxima])

HSP 1 Score: 1671.8 bits (4328), Expect = 0.0e+00
Identity = 862/900 (95.78%), Postives = 884/900 (98.22%), Query Frame = 0

Query: 1   APFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAFLEPSVILLILAANAAVGVI 60
           APFWKL+LKQFDDLLVKILIVAAVVSFLLALINGETG+ AFLEPSVIL+ILAANAAVGVI
Sbjct: 47  APFWKLILKQFDDLLVKILIVAAVVSFLLALINGETGIIAFLEPSVILMILAANAAVGVI 106

Query: 61  TETNAEKALEELRAYQADVATVMRNGCFSILPATELVPGDIVEVVVGCKIPADMRMIEML 120
           TETNAEKAL ELRAYQAD+ATVMRNGCFSILPATELVPGDIVEV VGCKIPADMRMIEML
Sbjct: 107 TETNAEKALVELRAYQADIATVMRNGCFSILPATELVPGDIVEVAVGCKIPADMRMIEML 166

Query: 121 SNQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVGAN 180
           SNQ+RVDQAILTGESCSVEKELE+TRA+NAVYQDKTNILFSGTVVVAGRARA+VVGVGAN
Sbjct: 167 SNQLRVDQAILTGESCSVEKELEATRAVNAVYQDKTNILFSGTVVVAGRARAVVVGVGAN 226

Query: 181 TAMGNIRDSILQTDDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGVVS 240
           TAMGNIRDSILQTDDE TPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGG++S
Sbjct: 227 TAMGNIRDSILQTDDEVTPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGILS 286

Query: 241 GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS 300
           GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS
Sbjct: 287 GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS 346

Query: 301 DKTGTLTTNMMSVSKICVVHSVLHGPQLSEYSVSGTTYAPDGIIFDSSGVQLEFPAQLPC 360
           DKTGTLTTNMMSVSKICVVHSVLHGPQLSEY+VSGTTYAPDG IFDS+GVQLEFPAQLPC
Sbjct: 347 DKTGTLTTNMMSVSKICVVHSVLHGPQLSEYNVSGTTYAPDGTIFDSTGVQLEFPAQLPC 406

Query: 361 ILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSK 420
           ILHMAMGSALCNESTLQYNPDKG+YEKIGESTEVALRVFAEKVGLPGFTSMP ALNMLSK
Sbjct: 407 ILHMAMGSALCNESTLQYNPDKGNYEKIGESTEVALRVFAEKVGLPGFTSMPFALNMLSK 466

Query: 421 HERASYCNHHWESQFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSILC 480
           HERASYCNHHWE+QFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSILC
Sbjct: 467 HERASYCNHHWENQFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSILC 526

Query: 481 NEDGSTSVLTASIRTELEARFQSFAGNEMLRCLAIAFKLIPASQQSLSFGDEQDLTFIGL 540
           NEDGSTSVLT+SIR ELEARFQSFAGNEMLRCLAIA KL+P SQQSLSF DEQDLTFIGL
Sbjct: 527 NEDGSTSVLTSSIRAELEARFQSFAGNEMLRCLAIAIKLLPVSQQSLSFDDEQDLTFIGL 586

Query: 541 VGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLTDHSFTAS 600
           VGMLDPPREEVRNAMLSCM AGIRVIVVTGDNKSTAESLCRKIGAFDHLVDL DHSFTAS
Sbjct: 587 VGMLDPPREEVRNAMLSCMNAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLADHSFTAS 646

Query: 601 EFEELPAMQQTIALQRMALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADI 660
           EFEELPAMQQT+ALQRMALFTRVEPSHK+MLVEALQHQNEVVAMTGDGVNDAPALKKADI
Sbjct: 647 EFEELPAMQQTMALQRMALFTRVEPSHKQMLVEALQHQNEVVAMTGDGVNDAPALKKADI 706

Query: 661 GIAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF 720
           GIAMGSGTAVAKSASDMVLADDNFA+IVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF
Sbjct: 707 GIAMGSGTAVAKSASDMVLADDNFATIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF 766

Query: 721 VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFR 780
           VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMK+KPRKVNEAVVTGWLFFR
Sbjct: 767 VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKSKPRKVNEAVVTGWLFFR 826

Query: 781 YLVIGAYVGLATIAGFIWWFIYSDSGPKLTYNELMNFDTCSTRETTYPCSIFEDRHPSTV 840
           YLVIGAYVGLATIAGFIWWFIYSDSGPKL Y ELM+FDTCSTR TTYPCSIFEDRHPSTV
Sbjct: 827 YLVIGAYVGLATIAGFIWWFIYSDSGPKLPYAELMSFDTCSTRATTYPCSIFEDRHPSTV 886

Query: 841 SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIFLTMILHMLIMYVRPLAVLFSV 900
           SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASI +TMILHMLIMYVRPLAVLFSV
Sbjct: 887 SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIVITMILHMLIMYVRPLAVLFSV 946

BLAST of MS001474 vs. NCBI nr
Match: KAG6585710.1 (Calcium-transporting ATPase 3, endoplasmic reticulum-type, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1671.4 bits (4327), Expect = 0.0e+00
Identity = 861/900 (95.67%), Postives = 884/900 (98.22%), Query Frame = 0

Query: 1   APFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAFLEPSVILLILAANAAVGVI 60
           APFWKL+LKQFDDLLVKILIVAAVVSFLLALINGETG+ AFLEPSVIL+ILAANAAVGVI
Sbjct: 81  APFWKLILKQFDDLLVKILIVAAVVSFLLALINGETGIIAFLEPSVILMILAANAAVGVI 140

Query: 61  TETNAEKALEELRAYQADVATVMRNGCFSILPATELVPGDIVEVVVGCKIPADMRMIEML 120
           TETNAEKAL ELRAYQAD+ATVMRNGCFSILPATELVPGDIVEV VGCKIPADMRMIEML
Sbjct: 141 TETNAEKALVELRAYQADIATVMRNGCFSILPATELVPGDIVEVAVGCKIPADMRMIEML 200

Query: 121 SNQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVGAN 180
           SNQ+RVDQAILTGESCSVEKELE+TRA+NAVYQDKTNILFSGTVVVAGRARA+VVGVGAN
Sbjct: 201 SNQLRVDQAILTGESCSVEKELEATRAVNAVYQDKTNILFSGTVVVAGRARAVVVGVGAN 260

Query: 181 TAMGNIRDSILQTDDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGVVS 240
           TAMGNIRDSILQTDDE TPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGG++S
Sbjct: 261 TAMGNIRDSILQTDDEVTPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGILS 320

Query: 241 GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS 300
           GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS
Sbjct: 321 GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS 380

Query: 301 DKTGTLTTNMMSVSKICVVHSVLHGPQLSEYSVSGTTYAPDGIIFDSSGVQLEFPAQLPC 360
           DKTGTLTTNMMSVSKICVVHSVLHGPQLSEY+VSGTTYAPDG IFDS+GVQLEFPAQLPC
Sbjct: 381 DKTGTLTTNMMSVSKICVVHSVLHGPQLSEYNVSGTTYAPDGTIFDSTGVQLEFPAQLPC 440

Query: 361 ILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSK 420
           ILHMAMGSALCNESTLQYNPDKG+YEKIGESTEVALRVFAEKVGLPGFTSMP ALNMLSK
Sbjct: 441 ILHMAMGSALCNESTLQYNPDKGNYEKIGESTEVALRVFAEKVGLPGFTSMPFALNMLSK 500

Query: 421 HERASYCNHHWESQFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSILC 480
           HERASYCNHHWE+QFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSS+LC
Sbjct: 501 HERASYCNHHWENQFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSVLC 560

Query: 481 NEDGSTSVLTASIRTELEARFQSFAGNEMLRCLAIAFKLIPASQQSLSFGDEQDLTFIGL 540
           NEDGSTSVLT+SIR ELEARFQSFAGNEMLRCLAIA KL+P SQQSLSF DEQDLTFIGL
Sbjct: 561 NEDGSTSVLTSSIRAELEARFQSFAGNEMLRCLAIAIKLLPVSQQSLSFDDEQDLTFIGL 620

Query: 541 VGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLTDHSFTAS 600
           VGMLDPPREEVRNAMLSCM AGIRVIVVTGDNKSTAESLCRKIGAFDHLVDL DHSFTAS
Sbjct: 621 VGMLDPPREEVRNAMLSCMNAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLADHSFTAS 680

Query: 601 EFEELPAMQQTIALQRMALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADI 660
           EFEELPAMQQT+ALQRMALFTRVEPSHK+MLVEALQHQNEVVAMTGDGVNDAPALKKADI
Sbjct: 681 EFEELPAMQQTMALQRMALFTRVEPSHKQMLVEALQHQNEVVAMTGDGVNDAPALKKADI 740

Query: 661 GIAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF 720
           GIAMGSGTAVAKSASDMVLADDNFA+IVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF
Sbjct: 741 GIAMGSGTAVAKSASDMVLADDNFATIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF 800

Query: 721 VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFR 780
           VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMK+KPRKVNEAVVTGWLFFR
Sbjct: 801 VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKSKPRKVNEAVVTGWLFFR 860

Query: 781 YLVIGAYVGLATIAGFIWWFIYSDSGPKLTYNELMNFDTCSTRETTYPCSIFEDRHPSTV 840
           YLVIGAYVGLATIAGFIWWFIYSDSGPKL Y ELM+FDTCSTR TTYPCSIFEDRHPSTV
Sbjct: 861 YLVIGAYVGLATIAGFIWWFIYSDSGPKLPYAELMSFDTCSTRATTYPCSIFEDRHPSTV 920

Query: 841 SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIFLTMILHMLIMYVRPLAVLFSV 900
           SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASI +TMILHMLIMYVRPLAVLFSV
Sbjct: 921 SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIVITMILHMLIMYVRPLAVLFSV 980

BLAST of MS001474 vs. NCBI nr
Match: XP_022951259.1 (calcium-transporting ATPase 3, endoplasmic reticulum-type [Cucurbita moschata])

HSP 1 Score: 1671.0 bits (4326), Expect = 0.0e+00
Identity = 860/900 (95.56%), Postives = 884/900 (98.22%), Query Frame = 0

Query: 1   APFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAFLEPSVILLILAANAAVGVI 60
           APFWKL+LKQFDDLLVKILIVAAVVSFLLALINGETG+ AFLEPSVIL+ILAANAAVGVI
Sbjct: 47  APFWKLILKQFDDLLVKILIVAAVVSFLLALINGETGIIAFLEPSVILMILAANAAVGVI 106

Query: 61  TETNAEKALEELRAYQADVATVMRNGCFSILPATELVPGDIVEVVVGCKIPADMRMIEML 120
           TETNAEKAL ELRAYQAD+ATVMRNGCFSILPATELVPGDIVEV VGCKIPADMRMIEML
Sbjct: 107 TETNAEKALVELRAYQADIATVMRNGCFSILPATELVPGDIVEVAVGCKIPADMRMIEML 166

Query: 121 SNQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVGAN 180
           SNQ+RVDQAILTGESCSVEKELE+TRA+NAVYQDKTNILFSGTVVVAGRARA+VVGVGAN
Sbjct: 167 SNQLRVDQAILTGESCSVEKELEATRAVNAVYQDKTNILFSGTVVVAGRARAVVVGVGAN 226

Query: 181 TAMGNIRDSILQTDDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGVVS 240
           TAMGNIRDSILQTDDE TPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGG++S
Sbjct: 227 TAMGNIRDSILQTDDEVTPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGILS 286

Query: 241 GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS 300
           GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS
Sbjct: 287 GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS 346

Query: 301 DKTGTLTTNMMSVSKICVVHSVLHGPQLSEYSVSGTTYAPDGIIFDSSGVQLEFPAQLPC 360
           DKTGTLTTNMMSVSKICVVHSVLHGPQLSEY++SGTTYAPDG IFDS+GVQLEFPAQLPC
Sbjct: 347 DKTGTLTTNMMSVSKICVVHSVLHGPQLSEYNISGTTYAPDGTIFDSTGVQLEFPAQLPC 406

Query: 361 ILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSK 420
           ILHMAMGSALCNESTLQYNPDKG+YEKIGESTEVALRVFAEKVGLPGFTSMP ALNMLSK
Sbjct: 407 ILHMAMGSALCNESTLQYNPDKGNYEKIGESTEVALRVFAEKVGLPGFTSMPFALNMLSK 466

Query: 421 HERASYCNHHWESQFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSILC 480
           HERASYCNHHWE+QFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSS+LC
Sbjct: 467 HERASYCNHHWENQFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSVLC 526

Query: 481 NEDGSTSVLTASIRTELEARFQSFAGNEMLRCLAIAFKLIPASQQSLSFGDEQDLTFIGL 540
           NEDGSTSVLT+SIR ELEARFQSFAGNEMLRCLAIA KL+P SQQSLSF DEQDLTFIGL
Sbjct: 527 NEDGSTSVLTSSIRAELEARFQSFAGNEMLRCLAIAIKLLPVSQQSLSFDDEQDLTFIGL 586

Query: 541 VGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLTDHSFTAS 600
           VGMLDPPREEVRNAMLSCM AGIRVIVVTGDNKSTAESLCRKIGAFDHLVDL DHSFTAS
Sbjct: 587 VGMLDPPREEVRNAMLSCMNAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLADHSFTAS 646

Query: 601 EFEELPAMQQTIALQRMALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADI 660
           EFEELPAMQQT+ALQRMALFTRVEPSHK+MLVEALQHQNEVVAMTGDGVNDAPALKKADI
Sbjct: 647 EFEELPAMQQTMALQRMALFTRVEPSHKQMLVEALQHQNEVVAMTGDGVNDAPALKKADI 706

Query: 661 GIAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF 720
           GIAMGSGTAVAKSASDMVLADDNFA+IVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF
Sbjct: 707 GIAMGSGTAVAKSASDMVLADDNFATIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF 766

Query: 721 VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFR 780
           VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMK+KPRKVNEAVVTGWLFFR
Sbjct: 767 VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKSKPRKVNEAVVTGWLFFR 826

Query: 781 YLVIGAYVGLATIAGFIWWFIYSDSGPKLTYNELMNFDTCSTRETTYPCSIFEDRHPSTV 840
           YLVIGAYVGLATIAGFIWWFIYSDSGPKL Y ELM+FDTCSTR TTYPCSIFEDRHPSTV
Sbjct: 827 YLVIGAYVGLATIAGFIWWFIYSDSGPKLPYAELMSFDTCSTRATTYPCSIFEDRHPSTV 886

Query: 841 SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIFLTMILHMLIMYVRPLAVLFSV 900
           SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASI +TMILHMLIMYVRPLAVLFSV
Sbjct: 887 SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIVITMILHMLIMYVRPLAVLFSV 946

BLAST of MS001474 vs. ExPASy Swiss-Prot
Match: Q9SY55 (Calcium-transporting ATPase 3, endoplasmic reticulum-type OS=Arabidopsis thaliana OX=3702 GN=ECA3 PE=2 SV=3)

HSP 1 Score: 1493.0 bits (3864), Expect = 0.0e+00
Identity = 764/899 (84.98%), Postives = 832/899 (92.55%), Query Frame = 0

Query: 2   PFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAFLEPSVILLILAANAAVGVIT 61
           PFWKLVLKQFDDLLVKILIVAA+VSF+LAL NGETG+TAFLEP VILLILAANAAVGVIT
Sbjct: 48  PFWKLVLKQFDDLLVKILIVAAIVSFVLALANGETGLTAFLEPFVILLILAANAAVGVIT 107

Query: 62  ETNAEKALEELRAYQADVATVMRNGCFSILPATELVPGDIVEVVVGCKIPADMRMIEMLS 121
           ETNAEKALEELRAYQA++ATV+RNGCFSILPATELVPGDIVEV VGCKIPAD+RMIEM S
Sbjct: 108 ETNAEKALEELRAYQANIATVLRNGCFSILPATELVPGDIVEVTVGCKIPADLRMIEMSS 167

Query: 122 NQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVGANT 181
           N  RVDQAILTGESCSVEK+++ T   NAVYQDK NILFSGT VVAGR RA+V+GVG+NT
Sbjct: 168 NTFRVDQAILTGESCSVEKDVDCTLTTNAVYQDKKNILFSGTDVVAGRGRAVVIGVGSNT 227

Query: 182 AMGNIRDSILQTDDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGVVSG 241
           AMG+I DS+LQTDDEATPLKKKLDEFG+FLAKVIAGIC LVW+VNIGHF DPSHGG   G
Sbjct: 228 AMGSIHDSMLQTDDEATPLKKKLDEFGSFLAKVIAGICVLVWVVNIGHFSDPSHGGFFKG 287

Query: 242 AIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICSD 301
           AIHYFKIAVALAVAAIPEGLPAVVTTCLALGTK+MARLNAIVRSLPSVETLGCTTVICSD
Sbjct: 288 AIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKKMARLNAIVRSLPSVETLGCTTVICSD 347

Query: 302 KTGTLTTNMMSVSKICVVHSVLHGPQLSEYSVSGTTYAPDGIIFDSSGVQLEFPAQLPCI 361
           KTGTLTTNMMSVSKICVV S  HGP ++E++VSGTTYAP+G +FDS+G+QL+ PAQ PC+
Sbjct: 348 KTGTLTTNMMSVSKICVVQSAEHGPMINEFTVSGTTYAPEGTVFDSNGMQLDLPAQSPCL 407

Query: 362 LHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSKH 421
            H+AM S+LCN+S LQYNPDK SYEKIGESTEVALRV AEKVGLPGF SMPSALNMLSKH
Sbjct: 408 HHLAMCSSLCNDSILQYNPDKDSYEKIGESTEVALRVLAEKVGLPGFDSMPSALNMLSKH 467

Query: 422 ERASYCNHHWESQFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSILCN 481
           ERASYCNH+WE+QFKK+ VLEF+RDRKMMS+LCS  Q  ++FSKGAPESII+RC+ ILCN
Sbjct: 468 ERASYCNHYWENQFKKVYVLEFTRDRKMMSVLCSHKQMDVMFSKGAPESIIARCNKILCN 527

Query: 482 EDGSTSVLTASIRTELEARFQSFAGNEMLRCLAIAFKLIPASQQSLSFGDEQDLTFIGLV 541
            DGS   LTA+ R ELE+RF SF G+E LRCLA+AFK +P  QQ++S+ +E DLTFIGLV
Sbjct: 528 GDGSVVPLTAAGRAELESRFYSF-GDETLRCLALAFKTVPHGQQTISYDNENDLTFIGLV 587

Query: 542 GMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLTDHSFTASE 601
           GMLDPPREEVR+AML+CMTAGIRVIVVTGDNKSTAESLCRKIGAFD+LVD +  S+TASE
Sbjct: 588 GMLDPPREEVRDAMLACMTAGIRVIVVTGDNKSTAESLCRKIGAFDNLVDFSGMSYTASE 647

Query: 602 FEELPAMQQTIALQRMALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADIG 661
           FE LPA+QQT+AL+RM LF+RVEPSHKRMLVEALQ QNEVVAMTGDGVNDAPALKKADIG
Sbjct: 648 FERLPAVQQTLALRRMTLFSRVEPSHKRMLVEALQKQNEVVAMTGDGVNDAPALKKADIG 707

Query: 662 IAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIFV 721
           IAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIFV
Sbjct: 708 IAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIFV 767

Query: 722 AAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFRY 781
           AAVLGIP+TLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKV EAVVTGWLFFRY
Sbjct: 768 AAVLGIPDTLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVGEAVVTGWLFFRY 827

Query: 782 LVIGAYVGLATIAGFIWWFIYSDSGPKLTYNELMNFDTCSTRETTYPCSIFEDRHPSTVS 841
           LVIG YVGLAT+AGFIWWF+YSD GPKLTY+ELMNF+TC+ RETTYPCSIFEDRHPSTV+
Sbjct: 828 LVIGVYVGLATVAGFIWWFVYSDGGPKLTYSELMNFETCALRETTYPCSIFEDRHPSTVA 887

Query: 842 MTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIFLTMILHMLIMYVRPLAVLFSV 901
           MTVLVVVEMFNALNNLSENQSLLVI P SNLWLV SI LTM+LH+LI+YV PLAVLFSV
Sbjct: 888 MTVLVVVEMFNALNNLSENQSLLVITPRSNLWLVGSIILTMLLHVLILYVHPLAVLFSV 945

BLAST of MS001474 vs. ExPASy Swiss-Prot
Match: P54209 (Cation-transporting ATPase CA1 OS=Dunaliella bioculata OX=13790 GN=CA1 PE=2 SV=1)

HSP 1 Score: 998.4 bits (2580), Expect = 5.1e-290
Identity = 521/900 (57.89%), Postives = 667/900 (74.11%), Query Frame = 0

Query: 2   PFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAFLEPSVILLILAANAAVGVIT 61
           P WKL+LKQFDDLLVKIL+ AA+V F++A+  GE+  +  +EP VILLIL ANA VGV+T
Sbjct: 71  PLWKLILKQFDDLLVKILLGAAIVDFIIAISEGESIQSGLIEPMVILLILVANATVGVVT 130

Query: 62  ETNAEKALEELRAYQADVATVMRNGCFSILPATELVPGDIVEVVVGCKIPADMRMIEMLS 121
           E NAEKA+E+L++Y+AD ATV+RNG   ++P+ ++VPGDIVE+ VG K+PAD R+  + +
Sbjct: 131 ERNAEKAIEQLKSYEADDATVLRNGQLQLIPSADIVPGDIVELAVGNKVPADTRVSHIYT 190

Query: 122 NQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVGANT 181
             +++DQ++LTGES +VEK  E      AVYQDK N+LFSGT+VVAGRAR IVVG G+NT
Sbjct: 191 TSLKIDQSLLTGESQAVEKHTEVVHNEQAVYQDKLNMLFSGTLVVAGRARGIVVGTGSNT 250

Query: 182 AMGNIRDSILQTDDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGVVSG 241
           A+G IRD++   +D  TPLK KLDEFG  L+KVIAGIC LVW+VNI  F DP+ GG   G
Sbjct: 251 AIGKIRDAMGVEEDVVTPLKAKLDEFGALLSKVIAGICVLVWVVNINRFNDPALGGWFQG 310

Query: 242 AIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICSD 301
           AIHYFKIAVALAVAAIPEGLPAVVTTCLALGT++MAR NAIVR+LPSVETLGCTTVICSD
Sbjct: 311 AIHYFKIAVALAVAAIPEGLPAVVTTCLALGTRKMARHNAIVRTLPSVETLGCTTVICSD 370

Query: 302 KTGTLTTNMMSVSKICVVHSVLHGPQLSEYSVSGTTYAPDGIIFDSSGVQLEFPAQLPCI 361
           KTGTLTTN MSV K+  V S     QL+E+ V+GTT++P+G++    GV L  PA  PC+
Sbjct: 371 KTGTLTTNQMSVIKVAAVQS--SSSQLAEFDVTGTTFSPEGMVLGPGGVVLRQPADTPCL 430

Query: 362 LHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSKH 421
            H A  +ALCN+S +      G+ ++IGESTE+ALRVFAEK+GL      PS++      
Sbjct: 431 AHAAQCAALCNDSQVFVAQKTGTLQRIGESTEIALRVFAEKIGL------PSSIRPDRPI 490

Query: 422 ERASY-CNHHWESQFKKISVLEFSRDRKMMSILC-SRNQSHILFSKGAPESIISRCSSIL 481
            R+ +  N+ W+   +++++LEFSRDRKMMS+L    ++ H ++SKGAPE ++ +CS +L
Sbjct: 491 SRSQFGTNNFWQEDVERLALLEFSRDRKMMSVLVKGSDRQHNIWSKGAPEFVLRKCSHVL 550

Query: 482 CNEDGSTSVLTASIRTELEARFQSFAGNEMLRCLAIAFKLIPASQQSLSFGDEQDLTFIG 541
            N       LT ++R  + +  Q+F   + LRCLA+AFK +P +   L + DE  LTFIG
Sbjct: 551 ANNGEGAVPLTDNMRQAILSDMQAFGSRQALRCLALAFKSVPTTTTKLDYSDESGLTFIG 610

Query: 542 LVGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLTDH---- 601
           L+GM DPPR E R+A+ +C  AGI+VI+VTGDNK TAE++ R++GA      L       
Sbjct: 611 LLGMHDPPRPECRSALSTCHNAGIKVIMVTGDNKGTAEAVARQVGALSPSTALAGSDDED 670

Query: 602 ----SFTASEFEELPAMQQTIALQRMALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVND 661
               S+T  EFEE+ A+ Q  A + + + +RVEP HK  LVE L+ Q  VVAMTGDGVND
Sbjct: 671 NLGISYTGREFEEMGALGQAAATRNLVVLSRVEPMHKLRLVELLKAQGHVVAMTGDGVND 730

Query: 662 APALKKADIGIAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISS 721
           APAL +ADIGIAMGSGTAVAK A+DMVL DDNFA+IV AVAEGR I+NNTKQFIRYMISS
Sbjct: 731 APALLRADIGIAMGSGTAVAKHAADMVLGDDNFATIVFAVAEGRVIFNNTKQFIRYMISS 790

Query: 722 NIGEVVCIFVAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEA 781
           NIGEVV IF+AA+LG+PE L PVQLLWVNLVTDGLPATA+GFN+ D D+M   PR+V++ 
Sbjct: 791 NIGEVVAIFLAALLGLPEVLTPVQLLWVNLVTDGLPATALGFNRADKDMMARGPRRVDDP 850

Query: 782 VVTGWLFFRYLVIGAYVGLATIAGFIWWFIYSDSGPKLTYNELMNFDTCSTRE-TTYPCS 841
           +V GWLF RYL+IG YVG+ T+ GFIWW+I    G  +T+++L +F  C+++      C 
Sbjct: 851 IVNGWLFLRYLIIGMYVGIVTVYGFIWWYISFPEGGNMTWSQLTHFQACASQPGGAKDCE 910

Query: 842 IFEDRHPSTVSMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIFLTMILHMLIMY 891
           +F  +HP+T+SM+VLVVVEMFNALNNLSE+ SLL IPPW N WLV +I  +M LH  I+Y
Sbjct: 911 VFHSKHPTTISMSVLVVVEMFNALNNLSEDSSLLRIPPWDNKWLVGAIATSMALHFGILY 962

BLAST of MS001474 vs. ExPASy Swiss-Prot
Match: O77696 (Sarcoplasmic/endoplasmic reticulum calcium ATPase 3 OS=Sus scrofa OX=9823 GN=ATP2A3 PE=2 SV=2)

HSP 1 Score: 911.0 bits (2353), Expect = 1.1e-263
Identity = 501/920 (54.46%), Postives = 655/920 (71.20%), Query Frame = 0

Query: 4   WKLVLKQFDDLLVKILIVAAVVSFLLALI-NGETGVTAFLEPSVILLILAANAAVGVITE 63
           W+LVL+QF+DLLV+IL++AA+VSF+LA    GE   TAF+EP VI+LIL ANA VGV  E
Sbjct: 50  WELVLEQFEDLLVRILLLAALVSFVLACFEEGEETTTAFVEPLVIVLILVANAVVGVWQE 109

Query: 64  TNAEKALEELRAYQADVATVMRNGCFSI--LPATELVPGDIVEVVVGCKIPADMRMIEML 123
            NAE A+E L+ Y+ ++  V+R+    +  + A ++VPGDIVEV VG K+PAD+R+IE+ 
Sbjct: 110 RNAENAIEALKEYEPEMGKVIRSDRKGVQRIRARDIVPGDIVEVAVGDKVPADLRLIEIK 169

Query: 124 SNQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVGAN 183
           S  +RVDQ+ILTGES SV K  ++     AV QDK N+LFSGT + +G+A  + V  G +
Sbjct: 170 STTLRVDQSILTGESVSVTKHTDAIPDPRAVNQDKKNMLFSGTNIASGKAVGVAVATGLH 229

Query: 184 TAMGNIRDSILQTDDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGV-V 243
           T +G IR+ +   + E TPL++KLDEFG  L++ I+ IC  VW++NIGHF DP+HGG  +
Sbjct: 230 TELGKIRNQMASVEPERTPLQQKLDEFGRQLSRAISVICMAVWVINIGHFADPAHGGSWL 289

Query: 244 SGAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVIC 303
            GA++YFKIAVALAVAAIPEGLPAV+TTCLALGT+RMAR NAIVRSLPSVETLGCT+VIC
Sbjct: 290 RGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMARKNAIVRSLPSVETLGCTSVIC 349

Query: 304 SDKTGTLTTNMMSVSKICVVHSVLHGP-QLSEYSVSGTTYAPDGIIFDSSGVQLEFPAQL 363
           SDKTGTLTTN MSV ++ VV     G  +L E+++SGTTYAP+G +    G Q     + 
Sbjct: 350 SDKTGTLTTNQMSVCRMFVVAEAEAGTCRLHEFTISGTTYAPEGEV--RQGEQPVRCGKF 409

Query: 364 PCILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNML 423
             ++ +A   ALCN+S L YN  KG YEK+GE+TE AL    EK+ +       + L  L
Sbjct: 410 DGLVELATICALCNDSALDYNEAKGVYEKVGEATETALTCLVEKMNV-----FDTDLQAL 469

Query: 424 SKHERASYCNHHWESQFKKISVLEFSRDRKMMSILCSRN------QSHILFSKGAPESII 483
           S+ ERA  CN   +   +K   LEFSRDRK MS+ C+        Q   +F KGAPES+I
Sbjct: 470 SRVERAGACNAVIKQLMRKEFTLEFSRDRKSMSVYCTPTRPGLVAQGSKMFVKGAPESVI 529

Query: 484 SRCSSILCNEDGSTSV-LTASIRTELEARFQSF-AGNEMLRCLAIAFKLIPASQQSLSFG 543
            RCSS+     GS +V L  + R ++ A+ + + +G++ LRCLA+A +  P  ++++   
Sbjct: 530 ERCSSV---RVGSRTVPLNTTSREQILAKVRDWGSGSDTLRCLALATRDAPPRKEAMQLD 589

Query: 544 D-------EQDLTFIGLVGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKI 603
           D       E DLTF+G VGMLDPPR EV + +  C  AGIRV+++TGDNK TA ++CR++
Sbjct: 590 DCSKFVQYETDLTFVGCVGMLDPPRPEVASCIARCRQAGIRVVMITGDNKGTAVAICRRL 649

Query: 604 GAFDHLVDLTDHSFTASEFEELPAMQQTIALQRMALFTRVEPSHKRMLVEALQHQNEVVA 663
           G  +   D+   ++T  EF++L   QQ  A +    F RVEP+HK  +VE LQ  NEV A
Sbjct: 650 GILEDTEDVVGKAYTGREFDDLSPEQQRHACRTARCFARVEPAHKSRIVENLQSFNEVTA 709

Query: 664 MTGDGVNDAPALKKADIGIAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQ 723
           MTGDGVNDAPALKKA+IGIAMGSGTAVAKSA++MVL+DDNFASIVAAV EGRAIY+N KQ
Sbjct: 710 MTGDGVNDAPALKKAEIGIAMGSGTAVAKSAAEMVLSDDNFASIVAAVEEGRAIYSNMKQ 769

Query: 724 FIRYMISSNIGEVVCIFVAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKA 783
           FIRY+ISSN+GEVVCIF+ A+LG+PE L PVQLLWVNLVTDGLPATA+GFN  D D+M+ 
Sbjct: 770 FIRYLISSNVGEVVCIFLTAILGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMEK 829

Query: 784 KPRKVNEAVVTGWLFFRYLVIGAYVGLATIAGFIWWFIYSDSGPKLTYNELMNFDTCSTR 843
           +PR   EA+++GWLFFRYL IG YVGLAT+A   WWF+Y   GP++T+ +L NF  CS  
Sbjct: 830 RPRNPREALISGWLFFRYLAIGVYVGLATVAAATWWFLYDAEGPQVTFYQLRNFLKCSED 889

Query: 844 E---TTYPCSIFEDRHPSTVSMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIFL 901
               T   C +FE R P+T++++VLV  EM NALN++SENQSLL +PPW N WL+A++ +
Sbjct: 890 NPLFTGTDCEVFESRFPTTMALSVLVTTEMCNALNSVSENQSLLRMPPWLNPWLLAAVAM 949

BLAST of MS001474 vs. ExPASy Swiss-Prot
Match: P18596 (Sarcoplasmic/endoplasmic reticulum calcium ATPase 3 OS=Rattus norvegicus OX=10116 GN=Atp2a3 PE=1 SV=3)

HSP 1 Score: 909.8 bits (2350), Expect = 2.4e-263
Identity = 500/920 (54.35%), Postives = 651/920 (70.76%), Query Frame = 0

Query: 4   WKLVLKQFDDLLVKILIVAAVVSFLLALI-NGETGVTAFLEPSVILLILAANAAVGVITE 63
           W+LV++QF+DLLV+IL++AA+VSF+LA    GE   TAF+EP VI+LIL ANA VGV  E
Sbjct: 50  WELVVEQFEDLLVRILLLAALVSFVLAWFEEGEETTTAFVEPLVIMLILVANAIVGVWQE 109

Query: 64  TNAEKALEELRAYQADVATVMRNGCFSI--LPATELVPGDIVEVVVGCKIPADMRMIEML 123
            NAE A+E L+ Y+ ++  V+R+    +  + A ++VPGDIVEV VG K+PAD+R+IE+ 
Sbjct: 110 RNAESAIEALKEYEPEMGKVIRSDRKGVQRIRARDIVPGDIVEVAVGDKVPADLRLIEIK 169

Query: 124 SNQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVGAN 183
           S  +RVDQ+ILTGES SV K  ++     AV QDK N+LFSGT + +G+A  + V  G +
Sbjct: 170 STTLRVDQSILTGESVSVTKHTDAIPDPRAVNQDKKNMLFSGTNIASGKALGVAVATGLH 229

Query: 184 TAMGNIRDSILQTDDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGV-V 243
           T +G IR  +   + E TPL++KLDEFG  L+  I+ IC  VW++NIGHF DP+HGG  +
Sbjct: 230 TELGKIRSQMAAVEPERTPLQRKLDEFGRQLSHAISVICVAVWVINIGHFADPAHGGSWL 289

Query: 244 SGAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVIC 303
            GA++YFKIAVALAVAAIPEGLPAV+TTCLALGT+RMAR NAIVRSLPSVETLGCT+VIC
Sbjct: 290 RGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMARKNAIVRSLPSVETLGCTSVIC 349

Query: 304 SDKTGTLTTNMMSVSKICVVHSVLHGP-QLSEYSVSGTTYAPDGIIFDSSGVQLEFPAQL 363
           SDKTGTLTTN MSV ++ VV     G  +L E+++SGTTY P+G +    G QL    Q 
Sbjct: 350 SDKTGTLTTNQMSVCRMFVVAEAEAGACRLHEFTISGTTYTPEGEV--RQGEQLVRCGQF 409

Query: 364 PCILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNML 423
             ++ +A   ALCN+S L YN  KG YEK+GE+TE AL    EK+ +       + L  L
Sbjct: 410 DGLVELATICALCNDSALDYNEAKGVYEKVGEATETALTCLVEKMNV-----FDTDLKGL 469

Query: 424 SKHERASYCNHHWESQFKKISVLEFSRDRKMMSILCS------RNQSHILFSKGAPESII 483
           S+ ERA  CN   +   +K   LEFSRDRK MS+ C+      + Q   +F KGAPES+I
Sbjct: 470 SRVERAGACNSVIKQLMQKEFTLEFSRDRKSMSVYCTPTRADPKAQGSKMFVKGAPESVI 529

Query: 484 SRCSSILCNEDGSTSV-LTASIRTELEARFQSF-AGNEMLRCLAIAFKLIPASQQSLSFG 543
            RCSS+     GS +V L+A+ R  + A+ + + +G+  LRCLA+A +  P  ++ +   
Sbjct: 530 ERCSSV---RVGSRTVPLSATSREHILAKIRDWGSGSHTLRCLALATRDTPPRKEDMQLD 589

Query: 544 D-------EQDLTFIGLVGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKI 603
           D       E  LTF+G VGMLDPPR EV   +  C  AGIRV+++TGDNK TA ++CR++
Sbjct: 590 DCSQFVQYETGLTFVGCVGMLDPPRPEVAACITRCSRAGIRVVMITGDNKGTAVAICRRL 649

Query: 604 GAFDHLVDLTDHSFTASEFEELPAMQQTIALQRMALFTRVEPSHKRMLVEALQHQNEVVA 663
           G F    D+   ++T  EF++L   QQ  A +    F RVEP+HK  +VE LQ  NE+ A
Sbjct: 650 GIFGDTEDVLGKAYTGREFDDLSPEQQRQACRTARCFARVEPAHKSRIVENLQSFNEITA 709

Query: 664 MTGDGVNDAPALKKADIGIAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQ 723
           MTGDGVNDAPALKKA+IGIAMGSGTAVAKSA++MVL+DDNFASIVAAV EGRAIYNN KQ
Sbjct: 710 MTGDGVNDAPALKKAEIGIAMGSGTAVAKSAAEMVLSDDNFASIVAAVEEGRAIYNNMKQ 769

Query: 724 FIRYMISSNIGEVVCIFVAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKA 783
           FIRY+ISSN+GEVVCIF+ A+LG+PE L PVQLLWVNLVTDGLPATA+GFN  D D+M+ 
Sbjct: 770 FIRYLISSNVGEVVCIFLTAILGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMEK 829

Query: 784 KPRKVNEAVVTGWLFFRYLVIGAYVGLATIAGFIWWFIYSDSGPKLTYNELMNFDTCSTR 843
            PR   EA+++GWLFFRYL IG YVGLAT+A   WWF+Y   GP++T+++L NF  CS  
Sbjct: 830 LPRNPREALISGWLFFRYLAIGVYVGLATVAAATWWFLYDAEGPQVTFHQLRNFLKCSED 889

Query: 844 ETTY---PCSIFEDRHPSTVSMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIFL 901
              +    C +FE R P+T++++VLV +EM NALN++SENQSLL +PPW N WL+ ++ +
Sbjct: 890 NPLFAGIDCEVFESRFPTTMALSVLVTIEMCNALNSVSENQSLLRMPPWLNPWLLGAVVM 949

BLAST of MS001474 vs. ExPASy Swiss-Prot
Match: O14983 (Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 OS=Homo sapiens OX=9606 GN=ATP2A1 PE=1 SV=1)

HSP 1 Score: 908.7 bits (2347), Expect = 5.3e-263
Identity = 501/921 (54.40%), Postives = 652/921 (70.79%), Query Frame = 0

Query: 4   WKLVLKQFDDLLVKILIVAAVVSFLLALI-NGETGVTAFLEPSVILLILAANAAVGVITE 63
           W+LV++QF+DLLV+IL++AA +SF+LA    GE  +TAF+EP VILLIL ANA VGV  E
Sbjct: 50  WELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILIANAIVGVWQE 109

Query: 64  TNAEKALEELRAYQADVATVMRNGCFSI--LPATELVPGDIVEVVVGCKIPADMRMIEML 123
            NAE A+E L+ Y+ ++  V R    S+  + A ++VPGDIVEV VG K+PAD+R++ + 
Sbjct: 110 RNAENAIEALKEYEPEMGKVYRADRKSVQRIKARDIVPGDIVEVAVGDKVPADIRILAIK 169

Query: 124 SNQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVGAN 183
           S  +RVDQ+ILTGES SV K  E      AV QDK N+LFSGT + AG+A  IV   G  
Sbjct: 170 STTLRVDQSILTGESVSVIKHTEPVPDPRAVNQDKKNMLFSGTNIAAGKALGIVATTGVG 229

Query: 184 TAMGNIRDSILQTDDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGV-V 243
           T +G IRD +  T+ + TPL++KLDEFG  L+KVI+ IC  VW++NIGHF DP HGG   
Sbjct: 230 TEIGKIRDQMAATEQDKTPLQQKLDEFGEQLSKVISLICVAVWLINIGHFNDPVHGGSWF 289

Query: 244 SGAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVIC 303
            GAI+YFKIAVALAVAAIPEGLPAV+TTCLALGT+RMA+ NAIVRSLPSVETLGCT+VIC
Sbjct: 290 RGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVIC 349

Query: 304 SDKTGTLTTNMMSVSKICVVHSVLHGP--QLSEYSVSGTTYAPDGIIFDSSGVQLEFPAQ 363
           SDKTGTLTTN MSV K+ ++  V  G    L+E+S++G+TYAP+G +  +   +   P Q
Sbjct: 350 SDKTGTLTTNQMSVCKMFIIDKV-DGDICLLNEFSITGSTYAPEGEVLKND--KPVRPGQ 409

Query: 364 LPCILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNM 423
              ++ +A   ALCN+S+L +N  KG YEK+GE+TE AL    EK+ +       + +  
Sbjct: 410 YDGLVELATICALCNDSSLDFNEAKGVYEKVGEATETALTTLVEKMNV-----FNTDVRS 469

Query: 424 LSKHERASYCNHHWESQFKKISVLEFSRDRKMMSILCSRNQS------HILFSKGAPESI 483
           LSK ERA+ CN       KK   LEFSRDRK MS+ CS  +S      + +F KGAPE +
Sbjct: 470 LSKVERANACNSVIRQLMKKEFTLEFSRDRKSMSVYCSPAKSSRAAVGNKMFVKGAPEGV 529

Query: 484 ISRCSSILCNEDGSTSV-LTASIRTELEARFQSF-AGNEMLRCLAIAFKLIPASQQSLSF 543
           I RC+ +     G+T V LT  ++ ++ A  + +  G + LRCLA+A +  P  ++ +  
Sbjct: 530 IDRCNYV---RVGTTRVPLTGPVKEKIMAVIKEWGTGRDTLRCLALATRDTPPKREEMVL 589

Query: 544 GD-------EQDLTFIGLVGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRK 603
            D       E DLTF+G+VGMLDPPR+EV  ++  C  AGIRVI++TGDNK TA ++CR+
Sbjct: 590 DDSARFLEYETDLTFVGVVGMLDPPRKEVTGSIQLCRDAGIRVIMITGDNKGTAIAICRR 649

Query: 604 IGAFDHLVDLTDHSFTASEFEELPAMQQTIALQRMALFTRVEPSHKRMLVEALQHQNEVV 663
           IG F    ++ D ++T  EF++LP  +Q  A +R   F RVEPSHK  +VE LQ  +E+ 
Sbjct: 650 IGIFGENEEVADRAYTGREFDDLPLAEQREACRRACCFARVEPSHKSKIVEYLQSYDEIT 709

Query: 664 AMTGDGVNDAPALKKADIGIAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTK 723
           AMTGDGVNDAPALKKA+IGIAMGSGTAVAK+AS+MVLADDNF++IVAAV EGRAIYNN K
Sbjct: 710 AMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMK 769

Query: 724 QFIRYMISSNIGEVVCIFVAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMK 783
           QFIRY+ISSN+GEVVCIF+ A LG+PE L PVQLLWVNLVTDGLPATA+GFN  D D+M 
Sbjct: 770 QFIRYLISSNVGEVVCIFLTAALGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMD 829

Query: 784 AKPRKVNEAVVTGWLFFRYLVIGAYVGLATIAGFIWWFIYSDSGPKLTYNELMNFDTCST 843
             PR   E +++GWLFFRY+ IG YVG AT+    WWF+Y++ GP + Y++L +F  C+ 
Sbjct: 830 RPPRSPKEPLISGWLFFRYMAIGGYVGAATVGAAAWWFLYAEDGPHVNYSQLTHFMQCTE 889

Query: 844 RETTY---PCSIFEDRHPSTVSMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIF 901
             T +    C +FE   P T++++VLV +EM NALN+LSENQSLL +PPW N+WL+ SI 
Sbjct: 890 DNTHFEGIDCEVFEAPEPMTMALSVLVTIEMCNALNSLSENQSLLRMPPWVNIWLLGSIC 949

BLAST of MS001474 vs. ExPASy TrEMBL
Match: A0A6J1BVC5 (Calcium-transporting ATPase OS=Momordica charantia OX=3673 GN=LOC111005059 PE=3 SV=1)

HSP 1 Score: 1727.2 bits (4472), Expect = 0.0e+00
Identity = 900/900 (100.00%), Postives = 900/900 (100.00%), Query Frame = 0

Query: 1   APFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAFLEPSVILLILAANAAVGVI 60
           APFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAFLEPSVILLILAANAAVGVI
Sbjct: 47  APFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAFLEPSVILLILAANAAVGVI 106

Query: 61  TETNAEKALEELRAYQADVATVMRNGCFSILPATELVPGDIVEVVVGCKIPADMRMIEML 120
           TETNAEKALEELRAYQADVATVMRNGCFSILPATELVPGDIVEVVVGCKIPADMRMIEML
Sbjct: 107 TETNAEKALEELRAYQADVATVMRNGCFSILPATELVPGDIVEVVVGCKIPADMRMIEML 166

Query: 121 SNQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVGAN 180
           SNQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVGAN
Sbjct: 167 SNQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVGAN 226

Query: 181 TAMGNIRDSILQTDDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGVVS 240
           TAMGNIRDSILQTDDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGVVS
Sbjct: 227 TAMGNIRDSILQTDDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGVVS 286

Query: 241 GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS 300
           GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS
Sbjct: 287 GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS 346

Query: 301 DKTGTLTTNMMSVSKICVVHSVLHGPQLSEYSVSGTTYAPDGIIFDSSGVQLEFPAQLPC 360
           DKTGTLTTNMMSVSKICVVHSVLHGPQLSEYSVSGTTYAPDGIIFDSSGVQLEFPAQLPC
Sbjct: 347 DKTGTLTTNMMSVSKICVVHSVLHGPQLSEYSVSGTTYAPDGIIFDSSGVQLEFPAQLPC 406

Query: 361 ILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSK 420
           ILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSK
Sbjct: 407 ILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSK 466

Query: 421 HERASYCNHHWESQFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSILC 480
           HERASYCNHHWESQFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSILC
Sbjct: 467 HERASYCNHHWESQFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSILC 526

Query: 481 NEDGSTSVLTASIRTELEARFQSFAGNEMLRCLAIAFKLIPASQQSLSFGDEQDLTFIGL 540
           NEDGSTSVLTASIRTELEARFQSFAGNEMLRCLAIAFKLIPASQQSLSFGDEQDLTFIGL
Sbjct: 527 NEDGSTSVLTASIRTELEARFQSFAGNEMLRCLAIAFKLIPASQQSLSFGDEQDLTFIGL 586

Query: 541 VGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLTDHSFTAS 600
           VGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLTDHSFTAS
Sbjct: 587 VGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLTDHSFTAS 646

Query: 601 EFEELPAMQQTIALQRMALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADI 660
           EFEELPAMQQTIALQRMALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADI
Sbjct: 647 EFEELPAMQQTIALQRMALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADI 706

Query: 661 GIAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF 720
           GIAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF
Sbjct: 707 GIAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF 766

Query: 721 VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFR 780
           VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFR
Sbjct: 767 VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFR 826

Query: 781 YLVIGAYVGLATIAGFIWWFIYSDSGPKLTYNELMNFDTCSTRETTYPCSIFEDRHPSTV 840
           YLVIGAYVGLATIAGFIWWFIYSDSGPKLTYNELMNFDTCSTRETTYPCSIFEDRHPSTV
Sbjct: 827 YLVIGAYVGLATIAGFIWWFIYSDSGPKLTYNELMNFDTCSTRETTYPCSIFEDRHPSTV 886

Query: 841 SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIFLTMILHMLIMYVRPLAVLFSV 900
           SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIFLTMILHMLIMYVRPLAVLFSV
Sbjct: 887 SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIFLTMILHMLIMYVRPLAVLFSV 946

BLAST of MS001474 vs. ExPASy TrEMBL
Match: A0A6J1KPB7 (Calcium-transporting ATPase OS=Cucurbita maxima OX=3661 GN=LOC111496395 PE=3 SV=1)

HSP 1 Score: 1671.8 bits (4328), Expect = 0.0e+00
Identity = 862/900 (95.78%), Postives = 884/900 (98.22%), Query Frame = 0

Query: 1   APFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAFLEPSVILLILAANAAVGVI 60
           APFWKL+LKQFDDLLVKILIVAAVVSFLLALINGETG+ AFLEPSVIL+ILAANAAVGVI
Sbjct: 47  APFWKLILKQFDDLLVKILIVAAVVSFLLALINGETGIIAFLEPSVILMILAANAAVGVI 106

Query: 61  TETNAEKALEELRAYQADVATVMRNGCFSILPATELVPGDIVEVVVGCKIPADMRMIEML 120
           TETNAEKAL ELRAYQAD+ATVMRNGCFSILPATELVPGDIVEV VGCKIPADMRMIEML
Sbjct: 107 TETNAEKALVELRAYQADIATVMRNGCFSILPATELVPGDIVEVAVGCKIPADMRMIEML 166

Query: 121 SNQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVGAN 180
           SNQ+RVDQAILTGESCSVEKELE+TRA+NAVYQDKTNILFSGTVVVAGRARA+VVGVGAN
Sbjct: 167 SNQLRVDQAILTGESCSVEKELEATRAVNAVYQDKTNILFSGTVVVAGRARAVVVGVGAN 226

Query: 181 TAMGNIRDSILQTDDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGVVS 240
           TAMGNIRDSILQTDDE TPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGG++S
Sbjct: 227 TAMGNIRDSILQTDDEVTPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGILS 286

Query: 241 GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS 300
           GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS
Sbjct: 287 GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS 346

Query: 301 DKTGTLTTNMMSVSKICVVHSVLHGPQLSEYSVSGTTYAPDGIIFDSSGVQLEFPAQLPC 360
           DKTGTLTTNMMSVSKICVVHSVLHGPQLSEY+VSGTTYAPDG IFDS+GVQLEFPAQLPC
Sbjct: 347 DKTGTLTTNMMSVSKICVVHSVLHGPQLSEYNVSGTTYAPDGTIFDSTGVQLEFPAQLPC 406

Query: 361 ILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSK 420
           ILHMAMGSALCNESTLQYNPDKG+YEKIGESTEVALRVFAEKVGLPGFTSMP ALNMLSK
Sbjct: 407 ILHMAMGSALCNESTLQYNPDKGNYEKIGESTEVALRVFAEKVGLPGFTSMPFALNMLSK 466

Query: 421 HERASYCNHHWESQFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSILC 480
           HERASYCNHHWE+QFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSILC
Sbjct: 467 HERASYCNHHWENQFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSILC 526

Query: 481 NEDGSTSVLTASIRTELEARFQSFAGNEMLRCLAIAFKLIPASQQSLSFGDEQDLTFIGL 540
           NEDGSTSVLT+SIR ELEARFQSFAGNEMLRCLAIA KL+P SQQSLSF DEQDLTFIGL
Sbjct: 527 NEDGSTSVLTSSIRAELEARFQSFAGNEMLRCLAIAIKLLPVSQQSLSFDDEQDLTFIGL 586

Query: 541 VGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLTDHSFTAS 600
           VGMLDPPREEVRNAMLSCM AGIRVIVVTGDNKSTAESLCRKIGAFDHLVDL DHSFTAS
Sbjct: 587 VGMLDPPREEVRNAMLSCMNAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLADHSFTAS 646

Query: 601 EFEELPAMQQTIALQRMALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADI 660
           EFEELPAMQQT+ALQRMALFTRVEPSHK+MLVEALQHQNEVVAMTGDGVNDAPALKKADI
Sbjct: 647 EFEELPAMQQTMALQRMALFTRVEPSHKQMLVEALQHQNEVVAMTGDGVNDAPALKKADI 706

Query: 661 GIAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF 720
           GIAMGSGTAVAKSASDMVLADDNFA+IVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF
Sbjct: 707 GIAMGSGTAVAKSASDMVLADDNFATIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF 766

Query: 721 VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFR 780
           VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMK+KPRKVNEAVVTGWLFFR
Sbjct: 767 VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKSKPRKVNEAVVTGWLFFR 826

Query: 781 YLVIGAYVGLATIAGFIWWFIYSDSGPKLTYNELMNFDTCSTRETTYPCSIFEDRHPSTV 840
           YLVIGAYVGLATIAGFIWWFIYSDSGPKL Y ELM+FDTCSTR TTYPCSIFEDRHPSTV
Sbjct: 827 YLVIGAYVGLATIAGFIWWFIYSDSGPKLPYAELMSFDTCSTRATTYPCSIFEDRHPSTV 886

Query: 841 SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIFLTMILHMLIMYVRPLAVLFSV 900
           SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASI +TMILHMLIMYVRPLAVLFSV
Sbjct: 887 SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIVITMILHMLIMYVRPLAVLFSV 946

BLAST of MS001474 vs. ExPASy TrEMBL
Match: A0A6J1GI63 (Calcium-transporting ATPase OS=Cucurbita moschata OX=3662 GN=LOC111454150 PE=3 SV=1)

HSP 1 Score: 1671.0 bits (4326), Expect = 0.0e+00
Identity = 860/900 (95.56%), Postives = 884/900 (98.22%), Query Frame = 0

Query: 1   APFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAFLEPSVILLILAANAAVGVI 60
           APFWKL+LKQFDDLLVKILIVAAVVSFLLALINGETG+ AFLEPSVIL+ILAANAAVGVI
Sbjct: 47  APFWKLILKQFDDLLVKILIVAAVVSFLLALINGETGIIAFLEPSVILMILAANAAVGVI 106

Query: 61  TETNAEKALEELRAYQADVATVMRNGCFSILPATELVPGDIVEVVVGCKIPADMRMIEML 120
           TETNAEKAL ELRAYQAD+ATVMRNGCFSILPATELVPGDIVEV VGCKIPADMRMIEML
Sbjct: 107 TETNAEKALVELRAYQADIATVMRNGCFSILPATELVPGDIVEVAVGCKIPADMRMIEML 166

Query: 121 SNQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVGAN 180
           SNQ+RVDQAILTGESCSVEKELE+TRA+NAVYQDKTNILFSGTVVVAGRARA+VVGVGAN
Sbjct: 167 SNQLRVDQAILTGESCSVEKELEATRAVNAVYQDKTNILFSGTVVVAGRARAVVVGVGAN 226

Query: 181 TAMGNIRDSILQTDDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGVVS 240
           TAMGNIRDSILQTDDE TPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGG++S
Sbjct: 227 TAMGNIRDSILQTDDEVTPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGILS 286

Query: 241 GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS 300
           GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS
Sbjct: 287 GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS 346

Query: 301 DKTGTLTTNMMSVSKICVVHSVLHGPQLSEYSVSGTTYAPDGIIFDSSGVQLEFPAQLPC 360
           DKTGTLTTNMMSVSKICVVHSVLHGPQLSEY++SGTTYAPDG IFDS+GVQLEFPAQLPC
Sbjct: 347 DKTGTLTTNMMSVSKICVVHSVLHGPQLSEYNISGTTYAPDGTIFDSTGVQLEFPAQLPC 406

Query: 361 ILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSK 420
           ILHMAMGSALCNESTLQYNPDKG+YEKIGESTEVALRVFAEKVGLPGFTSMP ALNMLSK
Sbjct: 407 ILHMAMGSALCNESTLQYNPDKGNYEKIGESTEVALRVFAEKVGLPGFTSMPFALNMLSK 466

Query: 421 HERASYCNHHWESQFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSILC 480
           HERASYCNHHWE+QFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSS+LC
Sbjct: 467 HERASYCNHHWENQFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSVLC 526

Query: 481 NEDGSTSVLTASIRTELEARFQSFAGNEMLRCLAIAFKLIPASQQSLSFGDEQDLTFIGL 540
           NEDGSTSVLT+SIR ELEARFQSFAGNEMLRCLAIA KL+P SQQSLSF DEQDLTFIGL
Sbjct: 527 NEDGSTSVLTSSIRAELEARFQSFAGNEMLRCLAIAIKLLPVSQQSLSFDDEQDLTFIGL 586

Query: 541 VGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLTDHSFTAS 600
           VGMLDPPREEVRNAMLSCM AGIRVIVVTGDNKSTAESLCRKIGAFDHLVDL DHSFTAS
Sbjct: 587 VGMLDPPREEVRNAMLSCMNAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLADHSFTAS 646

Query: 601 EFEELPAMQQTIALQRMALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADI 660
           EFEELPAMQQT+ALQRMALFTRVEPSHK+MLVEALQHQNEVVAMTGDGVNDAPALKKADI
Sbjct: 647 EFEELPAMQQTMALQRMALFTRVEPSHKQMLVEALQHQNEVVAMTGDGVNDAPALKKADI 706

Query: 661 GIAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF 720
           GIAMGSGTAVAKSASDMVLADDNFA+IVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF
Sbjct: 707 GIAMGSGTAVAKSASDMVLADDNFATIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF 766

Query: 721 VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFR 780
           VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMK+KPRKVNEAVVTGWLFFR
Sbjct: 767 VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKSKPRKVNEAVVTGWLFFR 826

Query: 781 YLVIGAYVGLATIAGFIWWFIYSDSGPKLTYNELMNFDTCSTRETTYPCSIFEDRHPSTV 840
           YLVIGAYVGLATIAGFIWWFIYSDSGPKL Y ELM+FDTCSTR TTYPCSIFEDRHPSTV
Sbjct: 827 YLVIGAYVGLATIAGFIWWFIYSDSGPKLPYAELMSFDTCSTRATTYPCSIFEDRHPSTV 886

Query: 841 SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIFLTMILHMLIMYVRPLAVLFSV 900
           SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASI +TMILHMLIMYVRPLAVLFSV
Sbjct: 887 SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIVITMILHMLIMYVRPLAVLFSV 946

BLAST of MS001474 vs. ExPASy TrEMBL
Match: A0A1S3BAL0 (Calcium-transporting ATPase OS=Cucumis melo OX=3656 GN=LOC103487824 PE=3 SV=1)

HSP 1 Score: 1668.7 bits (4320), Expect = 0.0e+00
Identity = 862/900 (95.78%), Postives = 889/900 (98.78%), Query Frame = 0

Query: 1   APFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAFLEPSVILLILAANAAVGVI 60
           APFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAFLEPSVIL+ILAANAAVGVI
Sbjct: 47  APFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAFLEPSVILMILAANAAVGVI 106

Query: 61  TETNAEKALEELRAYQADVATVMRNGCFSILPATELVPGDIVEVVVGCKIPADMRMIEML 120
           TETNAEKAL ELRAYQAD+ATV+RNGCFSILPATELVPGDIVEV VGCKIPADMRMIEML
Sbjct: 107 TETNAEKALLELRAYQADIATVLRNGCFSILPATELVPGDIVEVAVGCKIPADMRMIEML 166

Query: 121 SNQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVGAN 180
           ++Q+RVDQAILTGES SVEKELESTRA NAVYQDKTNILFSGTVVVAGRARAIVVGVGAN
Sbjct: 167 TSQLRVDQAILTGESSSVEKELESTRAANAVYQDKTNILFSGTVVVAGRARAIVVGVGAN 226

Query: 181 TAMGNIRDSILQTDDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGVVS 240
           TAMGNIRDSILQTDD+ TPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGV+S
Sbjct: 227 TAMGNIRDSILQTDDDVTPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGVLS 286

Query: 241 GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS 300
           GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS
Sbjct: 287 GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS 346

Query: 301 DKTGTLTTNMMSVSKICVVHSVLHGPQLSEYSVSGTTYAPDGIIFDSSGVQLEFPAQLPC 360
           DKTGTLTTNMMSVSKICVVHSV+HGPQLSEY+VSGTTYAPDGIIFD++GVQLE PAQLPC
Sbjct: 347 DKTGTLTTNMMSVSKICVVHSVVHGPQLSEYNVSGTTYAPDGIIFDNTGVQLEIPAQLPC 406

Query: 361 ILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSK 420
           ILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSK
Sbjct: 407 ILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSK 466

Query: 421 HERASYCNHHWESQFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSILC 480
           HERASYCNHHWESQFKKISVL+FSRDRKMMS+LCSRNQSHILFSKGAPESIISRCSSILC
Sbjct: 467 HERASYCNHHWESQFKKISVLDFSRDRKMMSVLCSRNQSHILFSKGAPESIISRCSSILC 526

Query: 481 NEDGSTSVLTASIRTELEARFQSFAGNEMLRCLAIAFKLIPASQQSLSFGDEQDLTFIGL 540
           NEDGST+VLT+SIRTELEARFQSFA NEMLRCLAIAFKL+P++QQSLSF DE+DLTFIGL
Sbjct: 527 NEDGSTTVLTSSIRTELEARFQSFAENEMLRCLAIAFKLLPSNQQSLSFDDEKDLTFIGL 586

Query: 541 VGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLTDHSFTAS 600
           VGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLT HS+TAS
Sbjct: 587 VGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLTGHSYTAS 646

Query: 601 EFEELPAMQQTIALQRMALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADI 660
           EFEELPAMQ+T+ALQ+MALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADI
Sbjct: 647 EFEELPAMQKTMALQQMALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADI 706

Query: 661 GIAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF 720
           GIAMGSGTAVAKSASDMVLADDNFA+IVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF
Sbjct: 707 GIAMGSGTAVAKSASDMVLADDNFATIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF 766

Query: 721 VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFR 780
           VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFR
Sbjct: 767 VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFR 826

Query: 781 YLVIGAYVGLATIAGFIWWFIYSDSGPKLTYNELMNFDTCSTRETTYPCSIFEDRHPSTV 840
           YLVIGAYVGLATIAGFIWWFIYSD+GPKLTY ELMNFDTCSTRETTYPCSIFEDRHPSTV
Sbjct: 827 YLVIGAYVGLATIAGFIWWFIYSDNGPKLTYTELMNFDTCSTRETTYPCSIFEDRHPSTV 886

Query: 841 SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIFLTMILHMLIMYVRPLAVLFSV 900
           SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASI LTMILHMLIMYV+PLAVLFSV
Sbjct: 887 SMTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIVLTMILHMLIMYVQPLAVLFSV 946

BLAST of MS001474 vs. ExPASy TrEMBL
Match: A0A1S4DWF4 (Calcium-transporting ATPase OS=Cucumis melo OX=3656 GN=LOC103487824 PE=3 SV=1)

HSP 1 Score: 1609.0 bits (4165), Expect = 0.0e+00
Identity = 828/864 (95.83%), Postives = 854/864 (98.84%), Query Frame = 0

Query: 1   APFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAFLEPSVILLILAANAAVGVI 60
           APFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAFLEPSVIL+ILAANAAVGVI
Sbjct: 47  APFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAFLEPSVILMILAANAAVGVI 106

Query: 61  TETNAEKALEELRAYQADVATVMRNGCFSILPATELVPGDIVEVVVGCKIPADMRMIEML 120
           TETNAEKAL ELRAYQAD+ATV+RNGCFSILPATELVPGDIVEV VGCKIPADMRMIEML
Sbjct: 107 TETNAEKALLELRAYQADIATVLRNGCFSILPATELVPGDIVEVAVGCKIPADMRMIEML 166

Query: 121 SNQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVGAN 180
           ++Q+RVDQAILTGES SVEKELESTRA NAVYQDKTNILFSGTVVVAGRARAIVVGVGAN
Sbjct: 167 TSQLRVDQAILTGESSSVEKELESTRAANAVYQDKTNILFSGTVVVAGRARAIVVGVGAN 226

Query: 181 TAMGNIRDSILQTDDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGVVS 240
           TAMGNIRDSILQTDD+ TPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGV+S
Sbjct: 227 TAMGNIRDSILQTDDDVTPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGVLS 286

Query: 241 GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS 300
           GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS
Sbjct: 287 GAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICS 346

Query: 301 DKTGTLTTNMMSVSKICVVHSVLHGPQLSEYSVSGTTYAPDGIIFDSSGVQLEFPAQLPC 360
           DKTGTLTTNMMSVSKICVVHSV+HGPQLSEY+VSGTTYAPDGIIFD++GVQLE PAQLPC
Sbjct: 347 DKTGTLTTNMMSVSKICVVHSVVHGPQLSEYNVSGTTYAPDGIIFDNTGVQLEIPAQLPC 406

Query: 361 ILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSK 420
           ILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSK
Sbjct: 407 ILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSK 466

Query: 421 HERASYCNHHWESQFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSILC 480
           HERASYCNHHWESQFKKISVL+FSRDRKMMS+LCSRNQSHILFSKGAPESIISRCSSILC
Sbjct: 467 HERASYCNHHWESQFKKISVLDFSRDRKMMSVLCSRNQSHILFSKGAPESIISRCSSILC 526

Query: 481 NEDGSTSVLTASIRTELEARFQSFAGNEMLRCLAIAFKLIPASQQSLSFGDEQDLTFIGL 540
           NEDGST+VLT+SIRTELEARFQSFA NEMLRCLAIAFKL+P++QQSLSF DE+DLTFIGL
Sbjct: 527 NEDGSTTVLTSSIRTELEARFQSFAENEMLRCLAIAFKLLPSNQQSLSFDDEKDLTFIGL 586

Query: 541 VGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLTDHSFTAS 600
           VGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLT HS+TAS
Sbjct: 587 VGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLTGHSYTAS 646

Query: 601 EFEELPAMQQTIALQRMALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADI 660
           EFEELPAMQ+T+ALQ+MALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADI
Sbjct: 647 EFEELPAMQKTMALQQMALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADI 706

Query: 661 GIAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF 720
           GIAMGSGTAVAKSASDMVLADDNFA+IVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF
Sbjct: 707 GIAMGSGTAVAKSASDMVLADDNFATIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIF 766

Query: 721 VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFR 780
           VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFR
Sbjct: 767 VAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFR 826

Query: 781 YLVIGAYVGLATIAGFIWWFIYSDSGPKLTYNELMNFDTCSTRETTYPCSIFEDRHPSTV 840
           YLVIGAYVGLATIAGFIWWFIYSD+GPKLTY ELMNFDTCSTRETTYPCSIFEDRHPSTV
Sbjct: 827 YLVIGAYVGLATIAGFIWWFIYSDNGPKLTYTELMNFDTCSTRETTYPCSIFEDRHPSTV 886

Query: 841 SMTVLVVVEMFNALNNLSENQSLL 865
           SMTVLVVVEMFNALNNLSENQSLL
Sbjct: 887 SMTVLVVVEMFNALNNLSENQSLL 910

BLAST of MS001474 vs. TAIR 10
Match: AT1G10130.1 (endoplasmic reticulum-type calcium-transporting ATPase 3 )

HSP 1 Score: 1493.0 bits (3864), Expect = 0.0e+00
Identity = 764/899 (84.98%), Postives = 832/899 (92.55%), Query Frame = 0

Query: 2   PFWKLVLKQFDDLLVKILIVAAVVSFLLALINGETGVTAFLEPSVILLILAANAAVGVIT 61
           PFWKLVLKQFDDLLVKILIVAA+VSF+LAL NGETG+TAFLEP VILLILAANAAVGVIT
Sbjct: 48  PFWKLVLKQFDDLLVKILIVAAIVSFVLALANGETGLTAFLEPFVILLILAANAAVGVIT 107

Query: 62  ETNAEKALEELRAYQADVATVMRNGCFSILPATELVPGDIVEVVVGCKIPADMRMIEMLS 121
           ETNAEKALEELRAYQA++ATV+RNGCFSILPATELVPGDIVEV VGCKIPAD+RMIEM S
Sbjct: 108 ETNAEKALEELRAYQANIATVLRNGCFSILPATELVPGDIVEVTVGCKIPADLRMIEMSS 167

Query: 122 NQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVGANT 181
           N  RVDQAILTGESCSVEK+++ T   NAVYQDK NILFSGT VVAGR RA+V+GVG+NT
Sbjct: 168 NTFRVDQAILTGESCSVEKDVDCTLTTNAVYQDKKNILFSGTDVVAGRGRAVVIGVGSNT 227

Query: 182 AMGNIRDSILQTDDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHGGVVSG 241
           AMG+I DS+LQTDDEATPLKKKLDEFG+FLAKVIAGIC LVW+VNIGHF DPSHGG   G
Sbjct: 228 AMGSIHDSMLQTDDEATPLKKKLDEFGSFLAKVIAGICVLVWVVNIGHFSDPSHGGFFKG 287

Query: 242 AIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSVETLGCTTVICSD 301
           AIHYFKIAVALAVAAIPEGLPAVVTTCLALGTK+MARLNAIVRSLPSVETLGCTTVICSD
Sbjct: 288 AIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKKMARLNAIVRSLPSVETLGCTTVICSD 347

Query: 302 KTGTLTTNMMSVSKICVVHSVLHGPQLSEYSVSGTTYAPDGIIFDSSGVQLEFPAQLPCI 361
           KTGTLTTNMMSVSKICVV S  HGP ++E++VSGTTYAP+G +FDS+G+QL+ PAQ PC+
Sbjct: 348 KTGTLTTNMMSVSKICVVQSAEHGPMINEFTVSGTTYAPEGTVFDSNGMQLDLPAQSPCL 407

Query: 362 LHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPGFTSMPSALNMLSKH 421
            H+AM S+LCN+S LQYNPDK SYEKIGESTEVALRV AEKVGLPGF SMPSALNMLSKH
Sbjct: 408 HHLAMCSSLCNDSILQYNPDKDSYEKIGESTEVALRVLAEKVGLPGFDSMPSALNMLSKH 467

Query: 422 ERASYCNHHWESQFKKISVLEFSRDRKMMSILCSRNQSHILFSKGAPESIISRCSSILCN 481
           ERASYCNH+WE+QFKK+ VLEF+RDRKMMS+LCS  Q  ++FSKGAPESII+RC+ ILCN
Sbjct: 468 ERASYCNHYWENQFKKVYVLEFTRDRKMMSVLCSHKQMDVMFSKGAPESIIARCNKILCN 527

Query: 482 EDGSTSVLTASIRTELEARFQSFAGNEMLRCLAIAFKLIPASQQSLSFGDEQDLTFIGLV 541
            DGS   LTA+ R ELE+RF SF G+E LRCLA+AFK +P  QQ++S+ +E DLTFIGLV
Sbjct: 528 GDGSVVPLTAAGRAELESRFYSF-GDETLRCLALAFKTVPHGQQTISYDNENDLTFIGLV 587

Query: 542 GMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLTDHSFTASE 601
           GMLDPPREEVR+AML+CMTAGIRVIVVTGDNKSTAESLCRKIGAFD+LVD +  S+TASE
Sbjct: 588 GMLDPPREEVRDAMLACMTAGIRVIVVTGDNKSTAESLCRKIGAFDNLVDFSGMSYTASE 647

Query: 602 FEELPAMQQTIALQRMALFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADIG 661
           FE LPA+QQT+AL+RM LF+RVEPSHKRMLVEALQ QNEVVAMTGDGVNDAPALKKADIG
Sbjct: 648 FERLPAVQQTLALRRMTLFSRVEPSHKRMLVEALQKQNEVVAMTGDGVNDAPALKKADIG 707

Query: 662 IAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIFV 721
           IAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIFV
Sbjct: 708 IAMGSGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIFV 767

Query: 722 AAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFRY 781
           AAVLGIP+TLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKV EAVVTGWLFFRY
Sbjct: 768 AAVLGIPDTLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVGEAVVTGWLFFRY 827

Query: 782 LVIGAYVGLATIAGFIWWFIYSDSGPKLTYNELMNFDTCSTRETTYPCSIFEDRHPSTVS 841
           LVIG YVGLAT+AGFIWWF+YSD GPKLTY+ELMNF+TC+ RETTYPCSIFEDRHPSTV+
Sbjct: 828 LVIGVYVGLATVAGFIWWFVYSDGGPKLTYSELMNFETCALRETTYPCSIFEDRHPSTVA 887

Query: 842 MTVLVVVEMFNALNNLSENQSLLVIPPWSNLWLVASIFLTMILHMLIMYVRPLAVLFSV 901
           MTVLVVVEMFNALNNLSENQSLLVI P SNLWLV SI LTM+LH+LI+YV PLAVLFSV
Sbjct: 888 MTVLVVVEMFNALNNLSENQSLLVITPRSNLWLVGSIILTMLLHVLILYVHPLAVLFSV 945

BLAST of MS001474 vs. TAIR 10
Match: AT1G07670.1 (endomembrane-type CA-ATPase 4 )

HSP 1 Score: 737.6 bits (1903), Expect = 1.2e-212
Identity = 453/962 (47.09%), Postives = 611/962 (63.51%), Query Frame = 0

Query: 4    WKLVLKQFDDLLVKILIVAAVVSFLLALIN----GETGVTAFLEPSVILLILAANAAVGV 63
            +KL+L+QF+D LV+IL+ AAV+SF+LA  +    GE G+TAF+EP VI LIL  NA VG+
Sbjct: 72   FKLILEQFNDTLVRILLAAAVISFVLAFFDGDEGGEMGITAFVEPLVIFLILIVNAIVGI 131

Query: 64   ITETNAEKALEELRAYQADVATVMRNGC-FSILPATELVPGDIVEVVVGCKIPADMRMIE 123
              ETNAEKALE L+  Q+  ATVMR+G   S LPA ELVPGDIVE+ VG K+PADMR++ 
Sbjct: 132  WQETNAEKALEALKEIQSQQATVMRDGTKVSSLPAKELVPGDIVELRVGDKVPADMRVVA 191

Query: 124  MLSNQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVG 183
            ++S+ +RV+Q  LTGES +V K  +     NA  Q K  ++F+GT VV G    +V   G
Sbjct: 192  LISSTLRVEQGSLTGESEAVSKTTKHVDE-NADIQGKKCMVFAGTTVVNGNCICLVTDTG 251

Query: 184  ANTAMGNIRDSILQT--DDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHG 243
             NT +G +   I +    +E TPLKKKL+EFG  L  +I  ICALVW++N+ +F    + 
Sbjct: 252  MNTEIGRVHSQIQEAAQHEEDTPLKKKLNEFGEVLTMIIGLICALVWLINVKYFLSWEY- 311

Query: 244  GVVSG-----------AIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRS 303
              V G             +YF+IAVALAVAAIPEGLPAV+TTCLALGT++MA+ NA+VR 
Sbjct: 312  --VDGWPRNFKFSFEKCTYYFEIAVALAVAAIPEGLPAVITTCLALGTRKMAQKNALVRK 371

Query: 304  LPSVETLGCTTVICSDKTGTLTTNMMSVSKICVVHSVLHGPQLSEYSVSGTTYAP-DGII 363
            LPSVETLGCTTVICSDKTGTLTTN M+VSK+  + S +    L  ++V GT++ P DG I
Sbjct: 372  LPSVETLGCTTVICSDKTGTLTTNQMAVSKLVAMGSRI--GTLRSFNVEGTSFDPRDGKI 431

Query: 364  FDSSGVQLEFPAQLPCILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVG 423
             D    +++   Q+     +A  +A+CN++ ++ +  +  +   G  TE AL+V  EK+G
Sbjct: 432  EDWPTGRMDANLQM-----IAKIAAICNDANVEKSDQQ--FVSRGMPTEAALKVLVEKMG 491

Query: 424  LPGFTSMPSALNMLSKHERASYCNHHWESQFKKISVLEFSRDRKMMSILC-SRNQSHILF 483
                   P  LN  S       C   W    ++I+ LEF RDRK M ++  S +   +L 
Sbjct: 492  ------FPEGLNEASSDGNVLRCCRLWSELEQRIATLEFDRDRKSMGVMVDSSSGKKLLL 551

Query: 484  SKGAPESIISRCSSILCNEDGSTSVLTASIRTELEARFQSFAGNEMLRCLAIAFKLI--- 543
             KGA E+++ R + I    DGST  L    R +L  +         LRCL  A+  +   
Sbjct: 552  VKGAVENVLERSTHIQL-LDGSTRELDQYSR-DLILQSLHDMSLSALRCLGFAYSDVPSD 611

Query: 544  ----------PASQQSLSFGD----EQDLTFIGLVGMLDPPREEVRNAMLSCMTAGIRVI 603
                      PA QQ L+  +    E +L F+G VG+ DPPR+EVR A+  C TAGIRV+
Sbjct: 612  FATYDGSEDHPAHQQLLNPSNYSSIESNLVFVGFVGLRDPPRKEVRQAIADCRTAGIRVM 671

Query: 604  VVTGDNKSTAESLCRKIGAFDHLVDLTDHSFTASEFEELPAMQQTIALQRMALFTRVEPS 663
            V+TGDNKSTAE++CR+IG F+   D++  S T  EF ++   +  +      LF+R EP 
Sbjct: 672  VITGDNKSTAEAICREIGVFEADEDISSRSLTGKEFMDVKDQKNHLRQTGGLLFSRAEPK 731

Query: 664  HKRMLVEALQHQNEVVAMTGDGVNDAPALKKADIGIAMG-SGTAVAKSASDMVLADDNFA 723
            HK+ +V  L+   EVVAMTGDGVNDAPALK ADIG+AMG SGT VAK ASD+VLADDNF+
Sbjct: 732  HKQEIVRLLKEDGEVVAMTGDGVNDAPALKLADIGVAMGISGTEVAKEASDLVLADDNFS 791

Query: 724  SIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIFVAAVLGIPETLAPVQLLWVNLVTDG 783
            +IVAAV EGR+IYNN K FIRYMISSNIGEV  IF+ A LGIPE + PVQLLWVNLVTDG
Sbjct: 792  TIVAAVGEGRSIYNNMKAFIRYMISSNIGEVASIFLTAALGIPEGMIPVQLLWVNLVTDG 851

Query: 784  LPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFRYLVIGAYVGLATIAGFIWWFIYS-- 843
             PATA+GFN  D D+MK  PR+ +++++T W+ FRY+VIG YVG+AT+  FI W+ ++  
Sbjct: 852  PPATALGFNPPDKDIMKKPPRRSDDSLITAWILFRYMVIGLYVGVATVGVFIIWYTHNSF 911

Query: 844  -------DSGPKLTYNELMNFDTCSTRE----------------TTYPCSIFEDR--HPS 901
                   D    ++Y++L ++  CS+ E                 + PC  F+      S
Sbjct: 912  MGIDLSQDGHSLVSYSQLAHWGQCSSWEGFKVSPFTAGSQTFSFDSNPCDYFQQGKIKAS 971

BLAST of MS001474 vs. TAIR 10
Match: AT1G07810.1 (ER-type Ca2+-ATPase 1 )

HSP 1 Score: 736.5 bits (1900), Expect = 2.6e-212
Identity = 453/962 (47.09%), Postives = 611/962 (63.51%), Query Frame = 0

Query: 4    WKLVLKQFDDLLVKILIVAAVVSFLLALIN----GETGVTAFLEPSVILLILAANAAVGV 63
            +KL+L+QF+D LV+IL+ AAV+SF+LA  +    GE G+TAF+EP VI LIL  NA VG+
Sbjct: 72   FKLILEQFNDTLVRILLAAAVISFVLAFFDGDEGGEMGITAFVEPLVIFLILIVNAIVGI 131

Query: 64   ITETNAEKALEELRAYQADVATVMRNGC-FSILPATELVPGDIVEVVVGCKIPADMRMIE 123
              ETNAEKALE L+  Q+  ATVMR+G   S LPA ELVPGDIVE+ VG K+PADMR++ 
Sbjct: 132  WQETNAEKALEALKEIQSQQATVMRDGTKVSSLPAKELVPGDIVELRVGDKVPADMRVVA 191

Query: 124  MLSNQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVG 183
            ++S+ +RV+Q  LTGES +V K  +     NA  Q K  ++F+GT VV G    +V   G
Sbjct: 192  LISSTLRVEQGSLTGESEAVSKTTKHVDE-NADIQGKKCMVFAGTTVVNGNCICLVTDTG 251

Query: 184  ANTAMGNIRDSILQT--DDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPSHG 243
             NT +G +   I +    +E TPLKKKL+EFG  L  +I  ICALVW++N+ +F    + 
Sbjct: 252  MNTEIGRVHSQIQEAAQHEEDTPLKKKLNEFGEVLTMIIGLICALVWLINVKYFLSWEY- 311

Query: 244  GVVSG-----------AIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRS 303
              V G             +YF+IAVALAVAAIPEGLPAV+TTCLALGT++MA+ NA+VR 
Sbjct: 312  --VDGWPRNFKFSFEKCTYYFEIAVALAVAAIPEGLPAVITTCLALGTRKMAQKNALVRK 371

Query: 304  LPSVETLGCTTVICSDKTGTLTTNMMSVSKICVVHSVLHGPQLSEYSVSGTTYAP-DGII 363
            LPSVETLGCTTVICSDKTGTLTTN M+VSK+  + S +    L  ++V GT++ P DG I
Sbjct: 372  LPSVETLGCTTVICSDKTGTLTTNQMAVSKLVAMGSRI--GTLRSFNVEGTSFDPRDGKI 431

Query: 364  FDSSGVQLEFPAQLPCILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVG 423
             D    +++   Q+     +A  +A+CN++ ++ +  +  +   G  TE AL+V  EK+G
Sbjct: 432  EDWPMGRMDANLQM-----IAKIAAICNDANVEQSDQQ--FVSRGMPTEAALKVLVEKMG 491

Query: 424  LPGFTSMPSALNMLSKHERASYCNHHWESQFKKISVLEFSRDRKMMSILC-SRNQSHILF 483
                   P  LN  S       C   W    ++I+ LEF RDRK M ++  S + + +L 
Sbjct: 492  ------FPEGLNEASSDGDVLRCCRLWSELEQRIATLEFDRDRKSMGVMVDSSSGNKLLL 551

Query: 484  SKGAPESIISRCSSILCNEDGSTSVLTASIRTELEARFQSFAGNEMLRCLAIAFKLI--- 543
             KGA E+++ R + I    DGS   L    R  +    +  +    LRCL  A+  +   
Sbjct: 552  VKGAVENVLERSTHIQL-LDGSKRELDQYSRDLILQSLRDMS-LSALRCLGFAYSDVPSD 611

Query: 544  ----------PASQQSLSFGD----EQDLTFIGLVGMLDPPREEVRNAMLSCMTAGIRVI 603
                      PA QQ L+  +    E +L F+G VG+ DPPR+EVR A+  C TAGIRV+
Sbjct: 612  FATYDGSEDHPAHQQLLNPSNYSSIESNLIFVGFVGLRDPPRKEVRQAIADCRTAGIRVM 671

Query: 604  VVTGDNKSTAESLCRKIGAFDHLVDLTDHSFTASEFEELPAMQQTIALQRMALFTRVEPS 663
            V+TGDNKSTAE++CR+IG F+   D++  S T  EF ++   +  +      LF+R EP 
Sbjct: 672  VITGDNKSTAEAICREIGVFEADEDISSRSLTGIEFMDVQDQKNHLRQTGGLLFSRAEPK 731

Query: 664  HKRMLVEALQHQNEVVAMTGDGVNDAPALKKADIGIAMG-SGTAVAKSASDMVLADDNFA 723
            HK+ +V  L+   EVVAMTGDGVNDAPALK ADIG+AMG SGT VAK ASDMVLADDNF+
Sbjct: 732  HKQEIVRLLKEDGEVVAMTGDGVNDAPALKLADIGVAMGISGTEVAKEASDMVLADDNFS 791

Query: 724  SIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIFVAAVLGIPETLAPVQLLWVNLVTDG 783
            +IVAAV EGR+IYNN K FIRYMISSNIGEV  IF+ A LGIPE + PVQLLWVNLVTDG
Sbjct: 792  TIVAAVGEGRSIYNNMKAFIRYMISSNIGEVASIFLTAALGIPEGMIPVQLLWVNLVTDG 851

Query: 784  LPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFRYLVIGAYVGLATIAGFIWWFIYS-- 843
             PATA+GFN  D D+MK  PR+ +++++T W+ FRY+VIG YVG+AT+  FI W+ +S  
Sbjct: 852  PPATALGFNPPDKDIMKKPPRRSDDSLITAWILFRYMVIGLYVGVATVGVFIIWYTHSSF 911

Query: 844  -------DSGPKLTYNELMNFDTCSTRE----------------TTYPCSIFEDR--HPS 901
                   D    ++Y++L ++  CS+ E                 + PC  F+      S
Sbjct: 912  MGIDLSQDGHSLVSYSQLAHWGQCSSWEGFKVSPFTAGSQTFSFDSNPCDYFQQGKIKAS 971

BLAST of MS001474 vs. TAIR 10
Match: AT4G00900.1 (ER-type Ca2+-ATPase 2 )

HSP 1 Score: 722.2 bits (1863), Expect = 5.0e-208
Identity = 456/977 (46.67%), Postives = 606/977 (62.03%), Query Frame = 0

Query: 2    PFWKLVLKQFDDLLVKILIVAAVVSFLLALINGE----TGVTAFLEPSVILLILAANAAV 61
            P W LVL+QFDD LVKIL+ AA +SF+LA +  E    +G  AF+EP VI+LIL  NA V
Sbjct: 53   PLWHLVLEQFDDTLVKILLGAAFISFVLAFLGEEHGSGSGFEAFVEPFVIVLILILNAVV 112

Query: 62   GVITETNAEKALEELRAYQADVATVMRNG-CFSILPATELVPGDIVEVVVGCKIPADMRM 121
            GV  E+NAEKALE L+  Q + A V+R+G     LPA ELVPGDIVE+ VG K+PADMR+
Sbjct: 113  GVWQESNAEKALEALKEMQCESAKVLRDGNVLPNLPARELVPGDIVELNVGDKVPADMRV 172

Query: 122  IEMLSNQIRVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVG 181
              + ++ +RV+Q+ LTGE+  V K        +   Q K N++F+GT VV G    IV  
Sbjct: 173  SGLKTSTLRVEQSSLTGEAMPVLKGANLVVMDDCELQGKENMVFAGTTVVNGSCVCIVTS 232

Query: 182  VGANTAMGNIRDSILQT--DDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHFRDPS 241
            +G +T +G I+  I +   ++  TPLKKKLDEFG+ L   I  +C LVW++N  +F    
Sbjct: 233  IGMDTEIGKIQRQIHEASLEESETPLKKKLDEFGSRLTTAICIVCVLVWMINYKNF---V 292

Query: 242  HGGVVSG------------AIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAI 301
               VV G              +YFKIAVALAVAAIPEGLPAV+TTCLALGT++MA+ NAI
Sbjct: 293  SWDVVDGYKPVNIKFSFEKCTYYFKIAVALAVAAIPEGLPAVITTCLALGTRKMAQKNAI 352

Query: 302  VRSLPSVETLGCTTVICSDKTGTLTTNMMSVSKICVVHSVLHGPQLSEYSVSGTTYAP-D 361
            VR LPSVETLGCTTVICSDKTGTLTTN MS ++   +       ++  +SVSGTTY P D
Sbjct: 353  VRKLPSVETLGCTTVICSDKTGTLTTNQMSATEFFTLGGKTTTTRV--FSVSGTTYDPKD 412

Query: 362  GIIFDSSGVQLEFPAQLPCILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAE 421
            G I D     ++  A L  +  +    ++CN++ + Y  +   +   G  TE AL+V  E
Sbjct: 413  GGIVDWGCNNMD--ANLQAVAEIC---SICNDAGVFY--EGKLFRATGLPTEAALKVLVE 472

Query: 422  KVGLPGFTSMPSALNMLSKHERAS----YCNHHWESQFKKISVLEFSRDRKMMSILCSR- 481
            K+G+P   +  +   + +  +  S     C   W  + KK++ LEF R RK MS++ S  
Sbjct: 473  KMGIPEKKNSENIEEVTNFSDNGSSVKLACCDWWNKRSKKVATLEFDRVRKSMSVIVSEP 532

Query: 482  NQSHILFSKGAPESIISRCSSILCNEDGSTSVLTASIRTELEARFQSFAGNEMLRCLAIA 541
            N  + L  KGA ESI+ R SS     DGS   L  S R E+  +  S   ++ LRCL +A
Sbjct: 533  NGQNRLLVKGAAESILER-SSFAQLADGSLVALDESSR-EVILKKHSEMTSKGLRCLGLA 592

Query: 542  FK--------------------LIPASQQSLSFGDEQDLTFIGLVGMLDPPREEVRNAML 601
            +K                    L P+S  ++    E +L F+G+VG+ DPPREEV  A+ 
Sbjct: 593  YKDELGEFSDYSSEEHPSHKKLLDPSSYSNI----ETNLIFVGVVGLRDPPREEVGRAIE 652

Query: 602  SCMTAGIRVIVVTGDNKSTAESLCRKIGAFDHLVDLTDHSFTASEFEELPAMQQTIALQR 661
             C  AGIRV+V+TGDNKSTAE++C +I  F    DL+  SFT  EF  LPA +++  L +
Sbjct: 653  DCRDAGIRVMVITGDNKSTAEAICCEIRLFSENEDLSQSSFTGKEFMSLPASRRSEILSK 712

Query: 662  MA--LFTRVEPSHKRMLVEALQHQNEVVAMTGDGVNDAPALKKADIGIAMG-SGTAVAKS 721
                +F+R EP HK+ +V  L+   E+VAMTGDGVNDAPALK ADIGIAMG +GT VAK 
Sbjct: 713  SGGKVFSRAEPRHKQEIVRMLKEMGEIVAMTGDGVNDAPALKLADIGIAMGITGTEVAKE 772

Query: 722  ASDMVLADDNFASIVAAVAEGRAIYNNTKQFIRYMISSNIGEVVCIFVAAVLGIPETLAP 781
            ASDMVLADDNF++IV+AVAEGR+IYNN K FIRYMISSN+GEV+ IF+ A LGIPE + P
Sbjct: 773  ASDMVLADDNFSTIVSAVAEGRSIYNNMKAFIRYMISSNVGEVISIFLTAALGIPECMIP 832

Query: 782  VQLLWVNLVTDGLPATAIGFNKQDSDVMKAKPRKVNEAVVTGWLFFRYLVIGAYVGLATI 841
            VQLLWVNLVTDG PATA+GFN  D D+MK  PRK ++ ++  W+  RYLVIG+YVG+AT+
Sbjct: 833  VQLLWVNLVTDGPPATALGFNPADIDIMKKPPRKSDDCLIDSWVLIRYLVIGSYVGVATV 892

Query: 842  AGFIWWF---------IYSDSGPKLTYNELMNFDTCSTRETTY----------------- 901
              F+ W+         + SD    +++ +L N+  CS+  T +                 
Sbjct: 893  GIFVLWYTQASFLGISLISDGHTLVSFTQLQNWSECSSWGTNFTATPYTVAGGLRTIAFE 952

BLAST of MS001474 vs. TAIR 10
Match: AT3G63380.1 (ATPase E1-E2 type family protein / haloacid dehalogenase-like hydrolase family protein )

HSP 1 Score: 311.2 bits (796), Expect = 2.7e-84
Identity = 252/805 (31.30%), Postives = 402/805 (49.94%), Query Frame = 0

Query: 7   VLKQFDDLLVKILIVAAVVSFLLALINGETGV-TAFLEPSVILLILAANAAVGVITETNA 66
           V + F DL + IL+V A+  F L     E G+   + E   I + +     V  ++    
Sbjct: 157 VYEAFKDLTILILLVCAI--FSLGFGIKEHGIKEGWYEGGSIFVAVFLVIVVSALSNFRQ 216

Query: 67  EKALEELRAYQADV-ATVMRNGCFSILPATELVPGDIVEVVVGCKIPADMRMIEMLSNQI 126
           E+  ++L     ++   V+R+     +   ++V GD+V + +G +IPAD   +E   + +
Sbjct: 217 ERQFDKLSKISNNIKVEVLRDSRRQHISIFDVVVGDVVFLKIGDQIPADGLFLE--GHSL 276

Query: 127 RVDQAILTGESCSVEKELESTRAINAVYQDKTNILFSGTVVVAGRARAIVVGVGANTAMG 186
           +VD++ +TGES  +E           V       LFSGT +V G A+ +VV VG +T  G
Sbjct: 277 QVDESSMTGESDHLE-----------VDHKDNPFLFSGTKIVDGFAQMLVVSVGMSTTWG 336

Query: 187 NIRDSILQTDDEATPLKKKLDEFGTFLAKVIAGICALVWIVNIGHF---------RDPSH 246
               SI Q   E TPL+ +LD   + + K+   + ALV +V +  +         +   +
Sbjct: 337 QTMSSINQDSSERTPLQVRLDTLTSTIGKIGLTVAALVLVVLLVRYFTGNTEKEGKREYN 396

Query: 247 GG------VVSGAIHYFKIAVALAVAAIPEGLPAVVTTCLALGTKRMARLNAIVRSLPSV 306
           G       VV+  +     AV + V AIPEGLP  VT  LA   KRM    A+VR L + 
Sbjct: 397 GSKTPVDTVVNSVVRIVAAAVTIVVVAIPEGLPLAVTLTLAYSMKRMMSDQAMVRKLSAC 456

Query: 307 ETLGCTTVICSDKTGTLTTNMMSVSKICVVHSVLHGPQLSEYSVSGTTYAPD--GIIFDS 366
           ET+G  TVIC+DKTGTLT N M V+K  +    +H         S    +PD   +++  
Sbjct: 457 ETMGSATVICTDKTGTLTLNEMKVTKFWLGQESIH-------EDSTKMISPDVLDLLYQG 516

Query: 367 SGVQLEFPAQLPCILHMAMGSALCNESTLQYNPDKGSYEKIGESTEVALRVFAEKVGLPG 426
           +G+                GS   ++S         + E  G  TE AL           
Sbjct: 517 TGLN-------------TTGSVCVSDS-------GSTPEFSGSPTEKALL---------S 576

Query: 427 FTSMPSALNMLSKHERASYCNHHWESQFKKISVLEFSRDRKMMSILCSR---NQSHILFS 486
           +T +   ++M S  ++            + + V  FS  +K   +L  R   N  H+ + 
Sbjct: 577 WTVLNLGMDMESVKQK-----------HEVLRVETFSSAKKRSGVLVRRKSDNTVHVHW- 636

Query: 487 KGAPESIISRCSSILCNEDGSTSVLTASIRTELEARFQSFAGNEMLRCLAIAFKLIPASQ 546
           KGA E +++ CS       GS  ++ ++ ++ ++A  Q  A +  LRC+A A K+  AS 
Sbjct: 637 KGAAEMVLAMCSHYY-TSTGSVDLMDSTAKSRIQAIIQGMAASS-LRCIAFAHKI--ASN 696

Query: 547 QSLSFGDEQDLTFIGLVGMLDPPREEVRNAMLSCMTAGIRVIVVTGDNKSTAESLCRKIG 606
            S+   +E  LT +G+VG+ DP R  V  A+ +C  AG+ + ++TGDN  TA+++  + G
Sbjct: 697 DSVL--EEDGLTLMGIVGLKDPCRPGVSKAVETCKLAGVTIKMITGDNVFTAKAIAFECG 756

Query: 607 AFDHL-VDLTDHSFTASEFEELPAMQQTIALQRMALFTRVEPSHKRMLVEALQHQNEVVA 666
             DH   D  D      +F      ++   + ++ +  R  PS K ++V+ L+ +  VVA
Sbjct: 757 ILDHNDKDEEDAVVEGVQFRNYTDEERMQKVDKIRVMARSSPSDKLLMVKCLRLKGHVVA 816

Query: 667 MTGDGVNDAPALKKADIGIAMG-SGTAVAKSASDMVLADDNFASIVAAVAEGRAIYNNTK 726
           +TGDG NDAPALK+ADIG++MG  GT VAK +SD+V+ DDNFAS+   +  GR +YNN +
Sbjct: 817 VTGDGTNDAPALKEADIGLSMGIQGTEVAKESSDIVILDDNFASVATVLKWGRCVYNNIQ 876

Query: 727 QFIRYMISSNIGEVVCIFVAAVLGIPETLAPVQLLWVNLVTDGLPATAIGFNKQDSDVMK 786
           +FI++ ++ N+  +V  F+AA+      L  VQLLWVNL+ D L A A+   +  ++++K
Sbjct: 877 KFIQFQLTVNVAALVINFIAAISAGEVPLTAVQLLWVNLIMDTLGALALATERPTNELLK 892

Query: 787 AKPRKVNEAVVTGWLFFRYLVIGAY 788
            KP    EA++T  ++   LV   Y
Sbjct: 937 RKPVGRTEALITNVMWRNLLVQSLY 892