MS001456 (gene) Bitter gourd (TR) v1

Overview
NameMS001456
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptiondnaJ protein P58IPK homolog
Locationscaffold36: 4286981 .. 4289349 (+)
RNA-Seq ExpressionMS001456
SyntenyMS001456
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCGACGACAATGGCAAACCGGATAAGGGGAATCAATGGCAATCGCCAAGAGCGCCGCACCAAGAGAAGGCGGAGGACATGAAACTGTGGGGAATTCTTGTTTTCGGTTTAATCGGTGCCTCTGCCACCACTCTCGCAGTGAGTTTTACGCATTTTTTTTCGTTATTTAGCCAACTTTCTGGTGATTAATTTCCAATTATCTTCGTTTCCTTTTTTCGTTACTTCTGGAATTTTATGCATTGGGCTACGTGTTCAGTAAATTTTTTTGGATAAGAATTGGAATAGTGCTAGACAATTCGAGTTTCAATCTATAATTAAATTCTTTATTCTGAAGAAGCAGAAGGCTGGCACCAGTATATGCTATTCAACTGATCATGAATGATAATAAATTATCCGAATCTTTATAGTTGAGTATGTTTGTTAATGGACTAATAAATGCTCTAATGCACTAATTTGTGGTGACATAAAAAATTTGCCTAGTAGTTTTTCTCCTTGCAGTTATTGCTTATCCGACTAGCTTTTGGATAACCCAGTTTGACACTGGTTGTTTTGGGCAGGTTAGTCAGCTGAGGAGAACTGTTGATTGGGTTTATACTCAGGTATATGCGTGTCTGCATTTAATTATGCTATTTAATATTTGCTAAACTCGTTTTACTAGAAGTGTACGCTTACAGTTCTACTGCTTATAAGTTGTATTTGGCATCTATGATCATATCACTTAGAAACGAAAACTTGCATTAAATAAAATGGAGAAAGAATTTGGTGATATATTGTTGAAATCTGGTAGCTGACAATATAATTGTTAAACAAAAGTTGAGCAGGTCACAATCATCATGGAGAGAAACTGGCCGAACTTTCCGGTCCTCATTTCAGGAGGAAGCATGGAAAAGGTATAATCGTCGGCTACAAGAGGAGTATGAAGAAGAAATGGAGAGGGTGGTAAGAATTCTTTAGCTATTTGTTATTGTCTACTCTTTTCATATAGCAGGAATAGTGTCTTCAATTTTCATTAGTCATTTTTTAAACTTCTAGGGACCTTGGAGGTGGTTCGAATGGAATTTATATGGCTTTCTTAGTTAATATGTGGTTTAAGTTTTACAGAAAGCTCGAGTGATGCTTTTTTGGCCAGAATATTATTGGACCCTTGTTGGTTAAGCTCAAGTTAACTTCTAAGTTCTCTCTCTCCCTGGATATTGGCGACACACTCCTTGCATTATGCTAATGTACCCACTTTAATCCCTGAGGTTTTGTGCACCCCTGTATCCATTCTGGAAGAAAACATGTCTTTTAAGATATTGAGTAATTACCATAGAACTTAGGACGTTGAAGCTATCTCCAAACATCCCAAGCCATTACCACTAGGGCAGCCCTTGGGTGGGTTCGAAACTTTCAAATTATTTGTCCGACCACAACTTATTTTACCTTCTTCCACGCCTTTATTTTATTTTATTTTTTGTCACTGTGGATTATTATCTCATCCTTTTGTTTTTTGTCATTGGACCTTATTGTTCATTGTTTGATTTAGGAGCGGATAAGGCGAATGCAGAATGTATTCAATAGAGAGAGAAACAAATACAAGAGAGGCTATGAAAGCTGGAGAGAGAATGGTCCTGGTGCGCATCATCAACATCACCCTCGAGATGATTGGTATTGGAAGGCTGAGACATTCTACAGAGAGCAAAGAAATAATTCTAGAGAAGCTCCAAGAGACAGAACAAGTTACTCGTTATCGCATCATTACTCAGTTTTGGGTCTAGACAGGTGAATATAATGCTAGATAACAGTACTTACATATAAACTATGCTTATTTTTTTCTTCCCCGTTCAATTTATTTATTTATTTATATATATTCTGATGAACTTAGGATTAAACCTACAAATTGGGTTTGATCTGCACTGTTCCTCCTTTTTCTATGTTGAGAGGTAGTTATGAGGTTACCTTACATCTTGTCTTAATGTCTGCTACAGGTCACGAAAACTGCCTTATACAGATGCTGAGATTAAGGTATGCTCCCAAAACTCATCTAAGCTCCTACATTATTTTACCAGAGTCAAGCGTTTGCTGTCGGTTTGTAGCTTTCTATGAAGTAATTTACTATTAAGGTTGCGGGTTCTGAATTGGACTATTCATGGTCACAGACAGCATTTCAATCCAAGGCAAAACAGTTCCATCCAGATCAGAACCAAGAAAATAAAGGTAGAACCGTAGAAGATGGTCTACTTCTTTTTGGCTTCCTTTTTACTTTTACTTTCAATTTTCACTTAGTCCCTTGTGATCGTTGTGTTGCAGAGGCTGCTGAGGCCAAGTTCAAAGAGGTGATGACATCTTATGAGGCCATAAAGTCAGAAAGAAAGAACAGTAGAGCG

mRNA sequence

ATGAGCGACGACAATGGCAAACCGGATAAGGGGAATCAATGGCAATCGCCAAGAGCGCCGCACCAAGAGAAGGCGGAGGACATGAAACTGTGGGGAATTCTTGTTTTCGGTTTAATCGGTGCCTCTGCCACCACTCTCGCAGTTAGTCAGCTGAGGAGAACTGTTGATTGGGTTTATACTCAGTTGAGCAGGTCACAATCATCATGGAGAGAAACTGGCCGAACTTTCCGGTCCTCATTTCAGGAGGAAGCATGGAAAAGGTATAATCGTCGGCTACAAGAGGAGTATGAAGAAGAAATGGAGAGGGTGGAGCGGATAAGGCGAATGCAGAATGTATTCAATAGAGAGAGAAACAAATACAAGAGAGGCTATGAAAGCTGGAGAGAGAATGGTCCTGGTGCGCATCATCAACATCACCCTCGAGATGATTGGTATTGGAAGGCTGAGACATTCTACAGAGAGCAAAGAAATAATTCTAGAGAAGCTCCAAGAGACAGAACAAGTTACTCGTTATCGCATCATTACTCAGTTTTGGGTCTAGACAGGTCACGAAAACTGCCTTATACAGATGCTGAGATTAAGACAGCATTTCAATCCAAGGCAAAACAGTTCCATCCAGATCAGAACCAAGAAAATAAAGAGGCTGCTGAGGCCAAGTTCAAAGAGGTGATGACATCTTATGAGGCCATAAAGTCAGAAAGAAAGAACAGTAGAGCG

Coding sequence (CDS)

ATGAGCGACGACAATGGCAAACCGGATAAGGGGAATCAATGGCAATCGCCAAGAGCGCCGCACCAAGAGAAGGCGGAGGACATGAAACTGTGGGGAATTCTTGTTTTCGGTTTAATCGGTGCCTCTGCCACCACTCTCGCAGTTAGTCAGCTGAGGAGAACTGTTGATTGGGTTTATACTCAGTTGAGCAGGTCACAATCATCATGGAGAGAAACTGGCCGAACTTTCCGGTCCTCATTTCAGGAGGAAGCATGGAAAAGGTATAATCGTCGGCTACAAGAGGAGTATGAAGAAGAAATGGAGAGGGTGGAGCGGATAAGGCGAATGCAGAATGTATTCAATAGAGAGAGAAACAAATACAAGAGAGGCTATGAAAGCTGGAGAGAGAATGGTCCTGGTGCGCATCATCAACATCACCCTCGAGATGATTGGTATTGGAAGGCTGAGACATTCTACAGAGAGCAAAGAAATAATTCTAGAGAAGCTCCAAGAGACAGAACAAGTTACTCGTTATCGCATCATTACTCAGTTTTGGGTCTAGACAGGTCACGAAAACTGCCTTATACAGATGCTGAGATTAAGACAGCATTTCAATCCAAGGCAAAACAGTTCCATCCAGATCAGAACCAAGAAAATAAAGAGGCTGCTGAGGCCAAGTTCAAAGAGGTGATGACATCTTATGAGGCCATAAAGTCAGAAAGAAAGAACAGTAGAGCG

Protein sequence

MSDDNGKPDKGNQWQSPRAPHQEKAEDMKLWGILVFGLIGASATTLAVSQLRRTVDWVYTQLSRSQSSWRETGRTFRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNKYKRGYESWRENGPGAHHQHHPRDDWYWKAETFYREQRNNSREAPRDRTSYSLSHHYSVLGLDRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSERKNSRA
Homology
BLAST of MS001456 vs. NCBI nr
Match: XP_022131686.1 (uncharacterized protein LOC111004802 [Momordica charantia])

HSP 1 Score: 466.8 bits (1200), Expect = 1.1e-127
Identity = 237/239 (99.16%), Postives = 237/239 (99.16%), Query Frame = 0

Query: 1   MSDDNGKPDKGNQWQSPRAPHQEKAEDMKLWGILVFGLIGASATTLAVSQLRRTVDWVYT 60
           MSDDNGKPDKGNQWQSPRAP QEKAEDMKLWGILVFGLIGASATTLAVSQLRRTVDWVYT
Sbjct: 1   MSDDNGKPDKGNQWQSPRAPRQEKAEDMKLWGILVFGLIGASATTLAVSQLRRTVDWVYT 60

Query: 61  QLSRSQSSWRETGRTFRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNKY 120
           QLSRSQSSWRETGRTFRSSFQEEAWKRYNRRLQEEY EEMERVERIRRMQNVFNRERNKY
Sbjct: 61  QLSRSQSSWRETGRTFRSSFQEEAWKRYNRRLQEEYXEEMERVERIRRMQNVFNRERNKY 120

Query: 121 KRGYESWRENGPGAHHQHHPRDDWYWKAETFYREQRNNSREAPRDRTSYSLSHHYSVLGL 180
           KRGYESWRENGPGAHHQHHPRDDWYWKAETFYREQRNNSREAPRDRTSYSLSHHYSVLGL
Sbjct: 121 KRGYESWRENGPGAHHQHHPRDDWYWKAETFYREQRNNSREAPRDRTSYSLSHHYSVLGL 180

Query: 181 DRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSERKNSRA 240
           DRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSERKNSRA
Sbjct: 181 DRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSERKNSRA 239

BLAST of MS001456 vs. NCBI nr
Match: XP_038886417.1 (uncharacterized protein LOC120076612 isoform X1 [Benincasa hispida])

HSP 1 Score: 417.5 bits (1072), Expect = 7.5e-113
Identity = 214/240 (89.17%), Postives = 225/240 (93.75%), Query Frame = 0

Query: 1   MSDDNGKPDKGNQWQSPRAPHQEKAEDMKLWGILVFGLIGASATTLAVSQLRRTVDWVYT 60
           M+DDNGKPDKGNQWQSPR PHQEKAEDMKLWGIL+FGLIGASATTLAV QLRRTVDWVYT
Sbjct: 1   MTDDNGKPDKGNQWQSPRTPHQEKAEDMKLWGILLFGLIGASATTLAVGQLRRTVDWVYT 60

Query: 61  QLSRSQSSW-RETGRTFRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNK 120
           QLSR+QSSW   +GR+FR+SFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNK
Sbjct: 61  QLSRTQSSWGGGSGRSFRTSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNK 120

Query: 121 YKRGYESWRENGPGAHHQHHPRDDWYWKAETFYREQ--RNNSREAPRDRTSYSLSHHYSV 180
           YKRGYESWRENGPGA+HQHH RDDWYWKAETFYREQ   NN RE PRDR SY LSHHY+V
Sbjct: 121 YKRGYESWRENGPGAYHQHHQRDDWYWKAETFYREQSNTNNYRETPRDRPSYLLSHHYTV 180

Query: 181 LGLDRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSERKNS 238
           LGL+R RK PYTDAEIKTAFQSKAKQFHPDQNQ+NKEAAEAKFKEVMTSYEAIKSERKN+
Sbjct: 181 LGLNRCRKTPYTDAEIKTAFQSKAKQFHPDQNQDNKEAAEAKFKEVMTSYEAIKSERKNN 240

BLAST of MS001456 vs. NCBI nr
Match: XP_008444515.1 (PREDICTED: uncharacterized protein LOC103487809 isoform X1 [Cucumis melo])

HSP 1 Score: 415.6 bits (1067), Expect = 2.9e-112
Identity = 213/243 (87.65%), Postives = 226/243 (93.00%), Query Frame = 0

Query: 1   MSDDNGKPDKGNQWQSPRAPHQEKAEDMKLWGILVFGLIGASATTLAVSQLRRTVDWVYT 60
           M+D+NGKPDKGNQWQSPR PHQEKAED+KLWGIL+FGLIGASATTLAV QLR+TVDWVYT
Sbjct: 1   MTDENGKPDKGNQWQSPRPPHQEKAEDVKLWGILLFGLIGASATTLAVGQLRKTVDWVYT 60

Query: 61  QLSRSQSSW-RETGRTFRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNK 120
           QLSR+QSSW   +GR+FRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNK
Sbjct: 61  QLSRTQSSWGAGSGRSFRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNK 120

Query: 121 YKRGYESWRENGPGAHHQHHPRDDWYWKAETFYREQR----NNSREAPRDRTSYSLSHHY 180
           YKRGYESWRENGPGA+HQHH RDDWYWKAETFYREQR    NN RE P DR SY LSHHY
Sbjct: 121 YKRGYESWRENGPGAYHQHHQRDDWYWKAETFYREQRQSNTNNYRETPTDRPSYLLSHHY 180

Query: 181 SVLGLDRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSERK 239
           +VLGL+R RK PYTDAEIKTAFQSKAKQFHPDQNQ+NKEAAEAKFKEVMTSYEAIKSERK
Sbjct: 181 TVLGLNRCRKTPYTDAEIKTAFQSKAKQFHPDQNQDNKEAAEAKFKEVMTSYEAIKSERK 240

BLAST of MS001456 vs. NCBI nr
Match: XP_008444516.1 (PREDICTED: uncharacterized protein LOC103487809 isoform X2 [Cucumis melo])

HSP 1 Score: 409.5 bits (1051), Expect = 2.1e-110
Identity = 212/243 (87.24%), Postives = 225/243 (92.59%), Query Frame = 0

Query: 1   MSDDNGKPDKGNQWQSPRAPHQEKAEDMKLWGILVFGLIGASATTLAVSQLRRTVDWVYT 60
           M+D+NGKPDKGNQWQSPR PHQEKAED+KLWGIL+FGLIGASATTLAV QLR+TVDWVYT
Sbjct: 1   MTDENGKPDKGNQWQSPRPPHQEKAEDVKLWGILLFGLIGASATTLAVGQLRKTVDWVYT 60

Query: 61  QLSRSQSSW-RETGRTFRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNK 120
           QLSR+QSSW   +GR+FRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNK
Sbjct: 61  QLSRTQSSWGAGSGRSFRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNK 120

Query: 121 YKRGYESWRENGPGAHHQHHPRDDWYWKAETFYREQR----NNSREAPRDRTSYSLSHHY 180
           YKRGYESWRENGPGA+HQHH RDDWYWKAETFYREQR    NN RE P DR SY LSHHY
Sbjct: 121 YKRGYESWRENGPGAYHQHHQRDDWYWKAETFYREQRQSNTNNYRETPTDRPSYLLSHHY 180

Query: 181 SVLGLDRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSERK 239
           +VLGL+R RK PYTDAEIKTAFQSKAKQFHPDQNQ+NK AAEAKFKEVMTSYEAIKSERK
Sbjct: 181 TVLGLNRCRKTPYTDAEIKTAFQSKAKQFHPDQNQDNK-AAEAKFKEVMTSYEAIKSERK 240

BLAST of MS001456 vs. NCBI nr
Match: XP_004142908.1 (uncharacterized protein LOC101213280 [Cucumis sativus] >KGN62419.1 hypothetical protein Csa_018626 [Cucumis sativus])

HSP 1 Score: 405.6 bits (1041), Expect = 3.0e-109
Identity = 210/243 (86.42%), Postives = 222/243 (91.36%), Query Frame = 0

Query: 1   MSDDNGKPDKGNQWQSPRAPHQEKAEDMKLWGILVFGLIGASATTLAVSQLRRTVDWVYT 60
           M+DDNGKPDKGNQWQSPR  HQEK EDMKLWGIL+FGLIGASATTLAV QLR+TVDWVYT
Sbjct: 1   MTDDNGKPDKGNQWQSPRPSHQEKEEDMKLWGILLFGLIGASATTLAVGQLRKTVDWVYT 60

Query: 61  QLSRSQSSW-RETGRTFRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNK 120
           QLSRSQSSW   TGR+FRSSFQE+AWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNK
Sbjct: 61  QLSRSQSSWGGGTGRSFRSSFQEDAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNK 120

Query: 121 YKRGYESWRENGPGAHHQHHPRDDWYWKAETFYREQR----NNSREAPRDRTSYSLSHHY 180
           YKRGYESWREN PG++HQH  RDDWYWKAETFYREQR    NN RE PR+  SY LSHHY
Sbjct: 121 YKRGYESWRENDPGSYHQHQQRDDWYWKAETFYREQRQSNTNNYRETPRNGPSYLLSHHY 180

Query: 181 SVLGLDRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSERK 239
           +VLGL+R RK PYTDAEIKTAFQSKAKQFHPDQNQ+NKEAAEAKFKEVMTSYEAIKSERK
Sbjct: 181 TVLGLNRCRKTPYTDAEIKTAFQSKAKQFHPDQNQDNKEAAEAKFKEVMTSYEAIKSERK 240

BLAST of MS001456 vs. ExPASy Swiss-Prot
Match: Q9HJ83 (Chaperone protein DnaJ OS=Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165) OX=273075 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 7.6e-07
Identity = 29/62 (46.77%), Postives = 42/62 (67.74%), Query Frame = 0

Query: 174 HYSVLGLDRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSE 233
           +Y +LG+DR+     TD EIK AF+  AK++HPD + ENK+ AE KFKE+  +YE +   
Sbjct: 5   YYKILGVDRNA----TDEEIKKAFRELAKKWHPDLHPENKQEAEEKFKEISEAYEVLSDP 62

Query: 234 RK 236
           +K
Sbjct: 65  QK 62

BLAST of MS001456 vs. ExPASy Swiss-Prot
Match: A3DF24 (Chaperone protein DnaJ OS=Hungateiclostridium thermocellum (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 9.9e-07
Identity = 31/65 (47.69%), Postives = 44/65 (67.69%), Query Frame = 0

Query: 174 HYSVLGLDRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSE 233
           +Y +LG+DR      +DAEIK A++  AKQ+HPD N  +K AAEAKFKE+  +YE +   
Sbjct: 7   YYEILGVDRGA----SDAEIKKAYRKLAKQYHPDMNPGDK-AAEAKFKEINEAYEVLSDP 66

Query: 234 RKNSR 239
           +K +R
Sbjct: 67  QKRAR 66

BLAST of MS001456 vs. ExPASy Swiss-Prot
Match: Q05646 (Chaperone protein DnaJ OS=Erysipelothrix rhusiopathiae OX=1648 GN=dnaJ PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.1e-05
Identity = 29/63 (46.03%), Postives = 42/63 (66.67%), Query Frame = 0

Query: 175 YSVLGLDRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSER 234
           Y +LG+ +S     TDAEIK A++  AK++HPD N+E  + AEAKFKEV  +YE +   +
Sbjct: 8   YEILGVSKSA----TDAEIKKAYRQLAKKYHPDINKE--DGAEAKFKEVQEAYEVLSDSQ 64

Query: 235 KNS 238
           K +
Sbjct: 68  KRA 64

BLAST of MS001456 vs. ExPASy Swiss-Prot
Match: Q316U7 (Chaperone protein DnaJ OS=Desulfovibrio alaskensis (strain ATCC BAA 1058 / DSM 17464 / G20) OX=207559 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 1.9e-05
Identity = 29/65 (44.62%), Postives = 41/65 (63.08%), Query Frame = 0

Query: 174 HYSVLGLDRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSE 233
           +Y VLG+ R       D EIK A++ KA +FHPD+N +N E AEAKFKE   +Y+ ++  
Sbjct: 6   YYEVLGVSRDA----ADDEIKRAYRKKAMEFHPDRNPDNPE-AEAKFKEAAEAYDVLRDA 65

Query: 234 RKNSR 239
            K +R
Sbjct: 66  EKRAR 65

BLAST of MS001456 vs. ExPASy Swiss-Prot
Match: A1BHL1 (Chaperone protein DnaJ OS=Chlorobium phaeobacteroides (strain DSM 266) OX=290317 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 3.2e-05
Identity = 31/65 (47.69%), Postives = 42/65 (64.62%), Query Frame = 0

Query: 174 HYSVLGLDRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSE 233
           +Y VLGL RS     T  EIK A++  A Q+HPD+N +NK+ AE  FKEV  +YEA+ ++
Sbjct: 5   YYEVLGLSRSA----TKDEIKKAYRKLAMQYHPDKNPDNKD-AEEHFKEVNEAYEALSND 64

Query: 234 RKNSR 239
            K  R
Sbjct: 65  DKRRR 64

BLAST of MS001456 vs. ExPASy TrEMBL
Match: A0A6J1BQ72 (uncharacterized protein LOC111004802 OS=Momordica charantia OX=3673 GN=LOC111004802 PE=4 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 5.2e-128
Identity = 237/239 (99.16%), Postives = 237/239 (99.16%), Query Frame = 0

Query: 1   MSDDNGKPDKGNQWQSPRAPHQEKAEDMKLWGILVFGLIGASATTLAVSQLRRTVDWVYT 60
           MSDDNGKPDKGNQWQSPRAP QEKAEDMKLWGILVFGLIGASATTLAVSQLRRTVDWVYT
Sbjct: 1   MSDDNGKPDKGNQWQSPRAPRQEKAEDMKLWGILVFGLIGASATTLAVSQLRRTVDWVYT 60

Query: 61  QLSRSQSSWRETGRTFRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNKY 120
           QLSRSQSSWRETGRTFRSSFQEEAWKRYNRRLQEEY EEMERVERIRRMQNVFNRERNKY
Sbjct: 61  QLSRSQSSWRETGRTFRSSFQEEAWKRYNRRLQEEYXEEMERVERIRRMQNVFNRERNKY 120

Query: 121 KRGYESWRENGPGAHHQHHPRDDWYWKAETFYREQRNNSREAPRDRTSYSLSHHYSVLGL 180
           KRGYESWRENGPGAHHQHHPRDDWYWKAETFYREQRNNSREAPRDRTSYSLSHHYSVLGL
Sbjct: 121 KRGYESWRENGPGAHHQHHPRDDWYWKAETFYREQRNNSREAPRDRTSYSLSHHYSVLGL 180

Query: 181 DRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSERKNSRA 240
           DRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSERKNSRA
Sbjct: 181 DRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSERKNSRA 239

BLAST of MS001456 vs. ExPASy TrEMBL
Match: A0A1S3BAG5 (uncharacterized protein LOC103487809 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487809 PE=4 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 1.4e-112
Identity = 213/243 (87.65%), Postives = 226/243 (93.00%), Query Frame = 0

Query: 1   MSDDNGKPDKGNQWQSPRAPHQEKAEDMKLWGILVFGLIGASATTLAVSQLRRTVDWVYT 60
           M+D+NGKPDKGNQWQSPR PHQEKAED+KLWGIL+FGLIGASATTLAV QLR+TVDWVYT
Sbjct: 1   MTDENGKPDKGNQWQSPRPPHQEKAEDVKLWGILLFGLIGASATTLAVGQLRKTVDWVYT 60

Query: 61  QLSRSQSSW-RETGRTFRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNK 120
           QLSR+QSSW   +GR+FRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNK
Sbjct: 61  QLSRTQSSWGAGSGRSFRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNK 120

Query: 121 YKRGYESWRENGPGAHHQHHPRDDWYWKAETFYREQR----NNSREAPRDRTSYSLSHHY 180
           YKRGYESWRENGPGA+HQHH RDDWYWKAETFYREQR    NN RE P DR SY LSHHY
Sbjct: 121 YKRGYESWRENGPGAYHQHHQRDDWYWKAETFYREQRQSNTNNYRETPTDRPSYLLSHHY 180

Query: 181 SVLGLDRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSERK 239
           +VLGL+R RK PYTDAEIKTAFQSKAKQFHPDQNQ+NKEAAEAKFKEVMTSYEAIKSERK
Sbjct: 181 TVLGLNRCRKTPYTDAEIKTAFQSKAKQFHPDQNQDNKEAAEAKFKEVMTSYEAIKSERK 240

BLAST of MS001456 vs. ExPASy TrEMBL
Match: A0A1S3BAJ7 (uncharacterized protein LOC103487809 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103487809 PE=4 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 9.9e-111
Identity = 212/243 (87.24%), Postives = 225/243 (92.59%), Query Frame = 0

Query: 1   MSDDNGKPDKGNQWQSPRAPHQEKAEDMKLWGILVFGLIGASATTLAVSQLRRTVDWVYT 60
           M+D+NGKPDKGNQWQSPR PHQEKAED+KLWGIL+FGLIGASATTLAV QLR+TVDWVYT
Sbjct: 1   MTDENGKPDKGNQWQSPRPPHQEKAEDVKLWGILLFGLIGASATTLAVGQLRKTVDWVYT 60

Query: 61  QLSRSQSSW-RETGRTFRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNK 120
           QLSR+QSSW   +GR+FRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNK
Sbjct: 61  QLSRTQSSWGAGSGRSFRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNK 120

Query: 121 YKRGYESWRENGPGAHHQHHPRDDWYWKAETFYREQR----NNSREAPRDRTSYSLSHHY 180
           YKRGYESWRENGPGA+HQHH RDDWYWKAETFYREQR    NN RE P DR SY LSHHY
Sbjct: 121 YKRGYESWRENGPGAYHQHHQRDDWYWKAETFYREQRQSNTNNYRETPTDRPSYLLSHHY 180

Query: 181 SVLGLDRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSERK 239
           +VLGL+R RK PYTDAEIKTAFQSKAKQFHPDQNQ+NK AAEAKFKEVMTSYEAIKSERK
Sbjct: 181 TVLGLNRCRKTPYTDAEIKTAFQSKAKQFHPDQNQDNK-AAEAKFKEVMTSYEAIKSERK 240

BLAST of MS001456 vs. ExPASy TrEMBL
Match: A0A0A0LN54 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G353470 PE=4 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 1.4e-109
Identity = 210/243 (86.42%), Postives = 222/243 (91.36%), Query Frame = 0

Query: 1   MSDDNGKPDKGNQWQSPRAPHQEKAEDMKLWGILVFGLIGASATTLAVSQLRRTVDWVYT 60
           M+DDNGKPDKGNQWQSPR  HQEK EDMKLWGIL+FGLIGASATTLAV QLR+TVDWVYT
Sbjct: 1   MTDDNGKPDKGNQWQSPRPSHQEKEEDMKLWGILLFGLIGASATTLAVGQLRKTVDWVYT 60

Query: 61  QLSRSQSSW-RETGRTFRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNK 120
           QLSRSQSSW   TGR+FRSSFQE+AWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNK
Sbjct: 61  QLSRSQSSWGGGTGRSFRSSFQEDAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNK 120

Query: 121 YKRGYESWRENGPGAHHQHHPRDDWYWKAETFYREQR----NNSREAPRDRTSYSLSHHY 180
           YKRGYESWREN PG++HQH  RDDWYWKAETFYREQR    NN RE PR+  SY LSHHY
Sbjct: 121 YKRGYESWRENDPGSYHQHQQRDDWYWKAETFYREQRQSNTNNYRETPRNGPSYLLSHHY 180

Query: 181 SVLGLDRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSERK 239
           +VLGL+R RK PYTDAEIKTAFQSKAKQFHPDQNQ+NKEAAEAKFKEVMTSYEAIKSERK
Sbjct: 181 TVLGLNRCRKTPYTDAEIKTAFQSKAKQFHPDQNQDNKEAAEAKFKEVMTSYEAIKSERK 240

BLAST of MS001456 vs. ExPASy TrEMBL
Match: A0A6J1KLR9 (dnaJ protein P58IPK homolog OS=Cucurbita maxima OX=3661 GN=LOC111496415 PE=4 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 7.1e-109
Identity = 211/242 (87.19%), Postives = 222/242 (91.74%), Query Frame = 0

Query: 1   MSDDNGKPDKGNQWQSPRAPHQEK-AEDMKLWGILVFGLIGASATTLAVSQLRRTVDWVY 60
           MSDDNGK DK NQWQSPRAPHQ+K  ED KLWG+L+FGLIGASATTLAVS LRRTVDWVY
Sbjct: 1   MSDDNGKSDKDNQWQSPRAPHQQKEEEDTKLWGVLLFGLIGASATTLAVSHLRRTVDWVY 60

Query: 61  TQLSRSQSSWR-ETGRTFRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERN 120
           TQLSRS+SSW    GR+FRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERN
Sbjct: 61  TQLSRSKSSWSGGNGRSFRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERN 120

Query: 121 KYKRGYESWRENGPGAHHQHHPRDDWYWKAETFYREQR-NNSREAPRDRTSYSLSHHYSV 180
           KYKRGYESWREN PGA+HQHH RDDWYWKAETFYREQ+ NN RE PR RTSY LSHHY+V
Sbjct: 121 KYKRGYESWRENDPGAYHQHHQRDDWYWKAETFYREQQSNNHRETPRVRTSYLLSHHYTV 180

Query: 181 LGLDRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSERKNS 240
           LGLDR RK PYTDAEIKTAFQSKAKQFHPDQNQ+NKEAAEAKFKEVMTSY+AIK ERK+S
Sbjct: 181 LGLDRCRKTPYTDAEIKTAFQSKAKQFHPDQNQDNKEAAEAKFKEVMTSYDAIKLERKSS 240

BLAST of MS001456 vs. TAIR 10
Match: AT4G07990.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 259.6 bits (662), Expect = 2.4e-69
Identity = 144/232 (62.07%), Postives = 173/232 (74.57%), Query Frame = 0

Query: 6   GKPDKGNQWQSPRAPHQEKAEDMKLWGILVFGLIGASATTLAVSQLRRTVDWVYTQLSRS 65
           G+ DK  +W  P  P Q+K  D + W +L   +I A+ATT AV QLRR  DWVYTQL+R+
Sbjct: 3   GEDDKDKKWNPP--PPQQK-PDSRPWEVLAAFVICATATTFAVHQLRRNFDWVYTQLTRT 62

Query: 66  QSSWRETGRTFRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNKYKRGYE 125
            S+ R    TFR+SFQEEAW+RYN+R+QEEYE+E+ERV RIRRMQ+VFNRERNK++RGYE
Sbjct: 63  PSAGR---GTFRTSFQEEAWRRYNKRMQEEYEDELERVARIRRMQSVFNRERNKFRRGYE 122

Query: 126 SWRENGPGA--HHQHHPRDDWYWKAETFYREQRNNSREAPRDRTSYSLSHHYSVLGLDRS 185
           +W EN PGA  +HQ   R DWYWK E+ +R QR N +E    R  Y LSHHYSVLGL RS
Sbjct: 123 NWTENDPGAQQYHQQFQRHDWYWKTESSHRNQRTNHQEPSDQRRVYPLSHHYSVLGLSRS 182

Query: 186 RKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSERK 236
           R  PYT+AEIK AF+ KA +FHPDQNQ+NK  AEAKFKEV+ SYEAIK E K
Sbjct: 183 RATPYTEAEIKKAFREKALEFHPDQNQDNKIVAEAKFKEVLLSYEAIKQEIK 228

BLAST of MS001456 vs. TAIR 10
Match: AT4G07990.2 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 227.6 bits (579), Expect = 1.0e-59
Identity = 133/232 (57.33%), Postives = 162/232 (69.83%), Query Frame = 0

Query: 6   GKPDKGNQWQSPRAPHQEKAEDMKLWGILVFGLIGASATTLAVSQLRRTVDWVYTQLSRS 65
           G+ DK  +W  P  P Q+K  D + W +L   +I A+ATT A              L+R+
Sbjct: 3   GEDDKDKKWNPP--PPQQK-PDSRPWEVLAAFVICATATTFA--------------LTRT 62

Query: 66  QSSWRETGRTFRSSFQEEAWKRYNRRLQEEYEEEMERVERIRRMQNVFNRERNKYKRGYE 125
            S+ R    TFR+SFQEEAW+RYN+R+QEEYE+E+ERV RIRRMQ+VFNRERNK++RGYE
Sbjct: 63  PSAGR---GTFRTSFQEEAWRRYNKRMQEEYEDELERVARIRRMQSVFNRERNKFRRGYE 122

Query: 126 SWRENGPGA--HHQHHPRDDWYWKAETFYREQRNNSREAPRDRTSYSLSHHYSVLGLDRS 185
           +W EN PGA  +HQ   R DWYWK E+ +R QR N +E    R  Y LSHHYSVLGL RS
Sbjct: 123 NWTENDPGAQQYHQQFQRHDWYWKTESSHRNQRTNHQEPSDQRRVYPLSHHYSVLGLSRS 182

Query: 186 RKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSERK 236
           R  PYT+AEIK AF+ KA +FHPDQNQ+NK  AEAKFKEV+ SYEAIK E K
Sbjct: 183 RATPYTEAEIKKAFREKALEFHPDQNQDNKIVAEAKFKEVLLSYEAIKQEIK 214

BLAST of MS001456 vs. TAIR 10
Match: AT5G03160.1 (homolog of mamallian P58IPK )

HSP 1 Score: 48.1 bits (113), Expect = 1.1e-05
Identity = 25/64 (39.06%), Postives = 41/64 (64.06%), Query Frame = 0

Query: 175 YSVLGLDRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSER 234
           Y +LG+ R+  +    +EIK A++  A Q+HPD+N  N+E AE KF+E+  +YE +  + 
Sbjct: 372 YKILGISRTASI----SEIKKAYKKLALQWHPDKNVGNREEAENKFREIAAAYEILGDDD 431

Query: 235 KNSR 239
           K +R
Sbjct: 432 KRAR 431

BLAST of MS001456 vs. TAIR 10
Match: AT5G01390.1 (DNAJ heat shock family protein )

HSP 1 Score: 47.8 bits (112), Expect = 1.5e-05
Identity = 25/63 (39.68%), Postives = 40/63 (63.49%), Query Frame = 0

Query: 175 YSVLGLDRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSER 234
           Y VL +DRS      D E+K A++  A ++HPD+N  NK+ AEAKFK++  +Y+ +   +
Sbjct: 6   YKVLEVDRSA----NDDELKKAYRKLAMKWHPDKNPNNKKEAEAKFKQISEAYDVLSDPQ 64

Query: 235 KNS 238
           K +
Sbjct: 66  KRA 64

BLAST of MS001456 vs. TAIR 10
Match: AT5G01390.2 (DNAJ heat shock family protein )

HSP 1 Score: 47.8 bits (112), Expect = 1.5e-05
Identity = 25/63 (39.68%), Postives = 40/63 (63.49%), Query Frame = 0

Query: 175 YSVLGLDRSRKLPYTDAEIKTAFQSKAKQFHPDQNQENKEAAEAKFKEVMTSYEAIKSER 234
           Y VL +DRS      D E+K A++  A ++HPD+N  NK+ AEAKFK++  +Y+ +   +
Sbjct: 6   YKVLEVDRSA----NDDELKKAYRKLAMKWHPDKNPNNKKEAEAKFKQISEAYDVLSDPQ 64

Query: 235 KNS 238
           K +
Sbjct: 66  KRA 64

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022131686.11.1e-12799.16uncharacterized protein LOC111004802 [Momordica charantia][more]
XP_038886417.17.5e-11389.17uncharacterized protein LOC120076612 isoform X1 [Benincasa hispida][more]
XP_008444515.12.9e-11287.65PREDICTED: uncharacterized protein LOC103487809 isoform X1 [Cucumis melo][more]
XP_008444516.12.1e-11087.24PREDICTED: uncharacterized protein LOC103487809 isoform X2 [Cucumis melo][more]
XP_004142908.13.0e-10986.42uncharacterized protein LOC101213280 [Cucumis sativus] >KGN62419.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
Q9HJ837.6e-0746.77Chaperone protein DnaJ OS=Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728... [more]
A3DF249.9e-0747.69Chaperone protein DnaJ OS=Hungateiclostridium thermocellum (strain ATCC 27405 / ... [more]
Q056461.1e-0546.03Chaperone protein DnaJ OS=Erysipelothrix rhusiopathiae OX=1648 GN=dnaJ PE=2 SV=1[more]
Q316U71.9e-0544.62Chaperone protein DnaJ OS=Desulfovibrio alaskensis (strain ATCC BAA 1058 / DSM 1... [more]
A1BHL13.2e-0547.69Chaperone protein DnaJ OS=Chlorobium phaeobacteroides (strain DSM 266) OX=290317... [more]
Match NameE-valueIdentityDescription
A0A6J1BQ725.2e-12899.16uncharacterized protein LOC111004802 OS=Momordica charantia OX=3673 GN=LOC111004... [more]
A0A1S3BAG51.4e-11287.65uncharacterized protein LOC103487809 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A1S3BAJ79.9e-11187.24uncharacterized protein LOC103487809 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A0A0LN541.4e-10986.42J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G353470 PE=4 SV=1[more]
A0A6J1KLR97.1e-10987.19dnaJ protein P58IPK homolog OS=Cucurbita maxima OX=3661 GN=LOC111496415 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
AT4G07990.12.4e-6962.07Chaperone DnaJ-domain superfamily protein [more]
AT4G07990.21.0e-5957.33Chaperone DnaJ-domain superfamily protein [more]
AT5G03160.11.1e-0539.06homolog of mamallian P58IPK [more]
AT5G01390.11.5e-0539.68DNAJ heat shock family protein [more]
AT5G01390.21.5e-0539.68DNAJ heat shock family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 82..109
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..23
NoneNo IPR availablePANTHERPTHR45000CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEINcoord: 1..237
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 197..212
score: 36.06
coord: 215..235
score: 36.83
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 172..235
e-value: 2.8E-12
score: 56.8
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 174..235
e-value: 5.3E-13
score: 48.9
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 173..239
score: 14.393378
IPR001623DnaJ domainCDDcd06257DnaJcoord: 174..228
e-value: 6.08529E-10
score: 51.7773
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 167..239
e-value: 5.9E-14
score: 53.9
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 170..236

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS001456.1MS001456.1mRNA