MS001389 (gene) Bitter gourd (TR) v1

Overview


Name	MS001389
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Endoglucanase
Location	scaffold36: 3808189 .. 3810257 (+)
RNA-Seq Expression	MS001389
Synteny	MS001389

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCACCACCAATCCACCATCCCCCTTGATCTCGATCTCGATCTTCGCCATGGGAGCCTTGAAGATGAAGCTTTCTACCACCGCCATTGTTGTGGGTTTTCTAGCTCTGTTCCAAACTTTTCTGGTTGATGCCACTGAATTGAATTACAAAGATGCCCTCAGCAAGGCCCTCATTTTCCTCGAAGCTCAGAGGTCTGGCAAGCTCCCTCCCAATCACCGCCCCGCTTGGAGAGGCGACTCCGCCCTCGACGACGGCAAACTCGCCAATGTCTGCTCATTCTTCCATTTTCTCCGCTCTCTCTGCCAACTCAAATTTCTGATTTTAACACGCTTTGAATTTGCAGGTTGACCTTGAGGGAGGGTATTACGACGCCGGCGACAATGTGAAGTACGGACTTCCGATGGCTTTCACGGTAACAACTCTGTCATGGGCGGCCATGACTTACCAAGCGGAGATCGAAGCCGCCGGCGAAATGGAAAATGTTCGAGCCGCAATCAAATGGGGCACCGATTATTTCCTCAAGGCCTGCTCTCGCCGCAATCGTCTCTATGTTCAGGTAACAGGGACATCCCCTGTTTTTGCCCTGACATTGGTTTGAAATGAACAAAAAATTGATGAAATTAAATTATTTATTTGATGGTGAAGGTGGGAGACCCGGTGAAGGATCATGAGTGTTGGGTTAGGCCTGAGAATATGAAGACGCCAAGAACTGTGCTGCAGATTGATAACAACACGCCCGGAACCGAAATTGCAGCTGAAACCTCCGCGGCCATGGCGTCTTCTTCCATTGTCTTCAGAAGCGTCAATAAAACCTATGCTCGTAGACTCCTAAACAAAGCAAAATTGGTACTAATTTCATTACTTCACACAAAATTTGCAAGTTCTGCCCTCTGGGATCAGGTAATTTTAACTAATTGGTAATCTTGTTCCCCCCTTCTCTGTTGCAGCTTTTTCAGTTCGCTAAAGCGCACAAAGGAACTTATGATGGAGAGTGCCCCTTCTATTGCTCTTTTTCAGGCTACAATGTATGTAACTCTCTAGTTATACGTCTGTTTTGCACGGCTTTATTTTGATTTCATAATAGCCCTGGTAGAACGTAGATAATTATACTCATCCATATAATATGAGTCATGCTTGAACATGAGATTTTGTTTGCATTCAATTTACAAACCCGACATTGTTACACACAGGACGAGTTGTTGTGGGCAGCGACATGGCTATACATCGCAACCAAGAGGCCAGTGTATCTGAAGTACGTGAAAGAAGAAGCCATTACTGGGAGTGTAGCGGAATTCAGCTGGGACCTCAAATATGCAGGCGCCCAAATTCTTCTTTCCAAGGTAAATTTCATCAAATTACAACAACACCGTTTCAAATTCTGAAAAGGAATTGTGGAATTGCAGTTCTATTTCGAGGGAGAGAAGGGATTACAGACGTTGAAGGATCAGGCGGATAGTTACATATGCTCCAATCTTCCAAACAGCCCTTACCACCAAATCTACGTATCCCCAGGAGGAATGATTCACATGAGAGATGGCGCCAACACCCAATACGTTACAGGAACGGCTTTCTTGTTCAGTGCTTACAGCGACATTCTTGCAACCTTTAAACAGACCGTCAAGTGCGCCGACCAGCAGTTCGATTCCACCCAGCTCATGTCTTTCGCTAAGAAACAGGTAATCAAACCCACAAAATCCACCGCCTTAATTAGCGATTAATTGTGTTTGAGATTTCTAATCGGAAATGGGTGCGCGCAGATGGATTATGTGCTGGGAATCAACCCGCTGGGGAGATCTTACATGGTGGGGTTCGGAAACAACCCGCCGGTTCAGGCGCACCACCGCGGCGCCTCCGTGCCGGTTCTGGCGCCGAACGCGGAAGTGAGCTGCCCGATGAGTTTCGTGGAGTGGTTCAACAAGAACACCCCCAACCCCAACGAGCTCACCGGCGCCATTCTCGGCGGCCCCGATAAGAACGACAACTTCGTCGATAAGCGTTGGGATTCGCCGATGACGGAGCCCGTCACTTACACCAACTCCCTCGCCGTCGGAGTCATCGCCAAGCTCGCC

mRNA sequence

TTCACCACCAATCCACCATCCCCCTTGATCTCGATCTCGATCTTCGCCATGGGAGCCTTGAAGATGAAGCTTTCTACCACCGCCATTGTTGTGGGTTTTCTAGCTCTGTTCCAAACTTTTCTGGTTGATGCCACTGAATTGAATTACAAAGATGCCCTCAGCAAGGCCCTCATTTTCCTCGAAGCTCAGAGGTCTGGCAAGCTCCCTCCCAATCACCGCCCCGCTTGGAGAGGCGACTCCGCCCTCGACGACGGCAAACTCGCCAATGTTGACCTTGAGGGAGGGTATTACGACGCCGGCGACAATGTGAAGTACGGACTTCCGATGGCTTTCACGGTAACAACTCTGTCATGGGCGGCCATGACTTACCAAGCGGAGATCGAAGCCGCCGGCGAAATGGAAAATGTTCGAGCCGCAATCAAATGGGGCACCGATTATTTCCTCAAGGCCTGCTCTCGCCGCAATCGTCTCTATGTTCAGGTGGGAGACCCGGTGAAGGATCATGAGTGTTGGGTTAGGCCTGAGAATATGAAGACGCCAAGAACTGTGCTGCAGATTGATAACAACACGCCCGGAACCGAAATTGCAGCTGAAACCTCCGCGGCCATGGCGTCTTCTTCCATTGTCTTCAGAAGCGTCAATAAAACCTATGCTCGTAGACTCCTAAACAAAGCAAAATTGCTTTTTCAGTTCGCTAAAGCGCACAAAGGAACTTATGATGGAGAGTGCCCCTTCTATTGCTCTTTTTCAGGCTACAATGACGAGTTGTTGTGGGCAGCGACATGGCTATACATCGCAACCAAGAGGCCAGTGTATCTGAAGTACGTGAAAGAAGAAGCCATTACTGGGAGTGTAGCGGAATTCAGCTGGGACCTCAAATATGCAGGCGCCCAAATTCTTCTTTCCAAGGTAAATTTCATCAAATTACAACAACACCAGAAGGGATTACAGACGTTGAAGGATCAGGCGGATAGTTACATATGCTCCAATCTTCCAAACAGCCCTTACCACCAAATCTACGTATCCCCAGGAGGAATGATTCACATGAGAGATGGCGCCAACACCCAATACGTTACAGGAACGGCTTTCTTGTTCAGTGCTTACAGCGACATTCTTGCAACCTTTAAACAGACCGTCAAGTGCGCCGACCAGCAGTTCGATTCCACCCAGCTCATGTCTTTCGCTAAGAAACAGATGGATTATGTGCTGGGAATCAACCCGCTGGGGAGATCTTACATGGTGGGGTTCGGAAACAACCCGCCGGTTCAGGCGCACCACCGCGGCGCCTCCGTGCCGGTTCTGGCGCCGAACGCGGAAGTGAGCTGCCCGATGAGTTTCGTGGAGTGGTTCAACAAGAACACCCCCAACCCCAACGAGCTCACCGGCGCCATTCTCGGCGGCCCCGATAAGAACGACAACTTCGTCGATAAGCGTTGGGATTCGCCGATGACGGAGCCCGTCACTTACACCAACTCCCTCGCCGTCGGAGTCATCGCCAAGCTCGCC

Coding sequence (CDS)

TTCACCACCAATCCACCATCCCCCTTGATCTCGATCTCGATCTTCGCCATGGGAGCCTTGAAGATGAAGCTTTCTACCACCGCCATTGTTGTGGGTTTTCTAGCTCTGTTCCAAACTTTTCTGGTTGATGCCACTGAATTGAATTACAAAGATGCCCTCAGCAAGGCCCTCATTTTCCTCGAAGCTCAGAGGTCTGGCAAGCTCCCTCCCAATCACCGCCCCGCTTGGAGAGGCGACTCCGCCCTCGACGACGGCAAACTCGCCAATGTTGACCTTGAGGGAGGGTATTACGACGCCGGCGACAATGTGAAGTACGGACTTCCGATGGCTTTCACGGTAACAACTCTGTCATGGGCGGCCATGACTTACCAAGCGGAGATCGAAGCCGCCGGCGAAATGGAAAATGTTCGAGCCGCAATCAAATGGGGCACCGATTATTTCCTCAAGGCCTGCTCTCGCCGCAATCGTCTCTATGTTCAGGTGGGAGACCCGGTGAAGGATCATGAGTGTTGGGTTAGGCCTGAGAATATGAAGACGCCAAGAACTGTGCTGCAGATTGATAACAACACGCCCGGAACCGAAATTGCAGCTGAAACCTCCGCGGCCATGGCGTCTTCTTCCATTGTCTTCAGAAGCGTCAATAAAACCTATGCTCGTAGACTCCTAAACAAAGCAAAATTGCTTTTTCAGTTCGCTAAAGCGCACAAAGGAACTTATGATGGAGAGTGCCCCTTCTATTGCTCTTTTTCAGGCTACAATGACGAGTTGTTGTGGGCAGCGACATGGCTATACATCGCAACCAAGAGGCCAGTGTATCTGAAGTACGTGAAAGAAGAAGCCATTACTGGGAGTGTAGCGGAATTCAGCTGGGACCTCAAATATGCAGGCGCCCAAATTCTTCTTTCCAAGGTAAATTTCATCAAATTACAACAACACCAGAAGGGATTACAGACGTTGAAGGATCAGGCGGATAGTTACATATGCTCCAATCTTCCAAACAGCCCTTACCACCAAATCTACGTATCCCCAGGAGGAATGATTCACATGAGAGATGGCGCCAACACCCAATACGTTACAGGAACGGCTTTCTTGTTCAGTGCTTACAGCGACATTCTTGCAACCTTTAAACAGACCGTCAAGTGCGCCGACCAGCAGTTCGATTCCACCCAGCTCATGTCTTTCGCTAAGAAACAGATGGATTATGTGCTGGGAATCAACCCGCTGGGGAGATCTTACATGGTGGGGTTCGGAAACAACCCGCCGGTTCAGGCGCACCACCGCGGCGCCTCCGTGCCGGTTCTGGCGCCGAACGCGGAAGTGAGCTGCCCGATGAGTTTCGTGGAGTGGTTCAACAAGAACACCCCCAACCCCAACGAGCTCACCGGCGCCATTCTCGGCGGCCCCGATAAGAACGACAACTTCGTCGATAAGCGTTGGGATTCGCCGATGACGGAGCCCGTCACTTACACCAACTCCCTCGCCGTCGGAGTCATCGCCAAGCTCGCC

Protein sequence

FTTNPPSPLISISIFAMGALKMKLSTTAIVVGFLALFQTFLVDATELNYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSALDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDELLWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKVNFIKLQQHQKGLQTLKDQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQTVKCADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPNAEVSCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKLA

Homology

[Close]

BLAST of MS001389 vs. NCBI nr
Match: XP_022131779.1 (endoglucanase 16 [Momordica charantia])

HSP 1 Score: 964.1 bits (2491), Expect = 4.5e-277
Identity = 475/486 (97.74%), Postives = 478/486 (98.35%), Query Frame = 0


Query: 17  MGALKMKLSTTAIVVGFLALFQTFLVDATELNYKDALSKALIFLEAQRSGKLPPNHRPAW 76
           MGALKMKLSTTAI VGFLALFQTFLVDATELNYKDALSKALIFLEAQRSGKLPPNHRPAW
Sbjct: 1   MGALKMKLSTTAIAVGFLALFQTFLVDATELNYKDALSKALIFLEAQRSGKLPPNHRPAW 60

Query: 77  RGDSALDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENV 136
           RGDSA+DDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENV
Sbjct: 61  RGDSAVDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENV 120

Query: 137 RAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIA 196
           RAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIA
Sbjct: 121 RAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIA 180

Query: 197 AETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDEL 256
           AETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDEL
Sbjct: 181 AETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDEL 240

Query: 257 LWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKVNFIKLQQHQKGL 316
           LWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSK  F    + +KGL
Sbjct: 241 LWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKFYF----EGEKGL 300

Query: 317 QTLKDQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFK 376
           QTLKDQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFK
Sbjct: 301 QTLKDQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFK 360

Query: 377 QTVKCADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAP 436
           QTVKCADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAP
Sbjct: 361 QTVKCADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAP 420

Query: 437 NAEVSCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVG 496
           NAEVSCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVG
Sbjct: 421 NAEVSCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVG 480

Query: 497 VIAKLA 503
           VIAKLA
Sbjct: 481 VIAKLA 482

[Close]

BLAST of MS001389 vs. NCBI nr
Match: XP_038886295.1 (endoglucanase 16 [Benincasa hispida])

HSP 1 Score: 852.4 bits (2201), Expect = 1.9e-243
Identity = 414/491 (84.32%), Postives = 455/491 (92.67%), Query Frame = 0


Query: 17  MGALKMKLSTTAIV-VGFLALFQTFL----VDATELNYKDALSKALIFLEAQRSGKLPPN 76
           MGA+K KLSTT I+ + ++  FQ  +    V+A +LNYKDAL+K+LIFLEAQRSGKLPPN
Sbjct: 1   MGAMKNKLSTTPIILLAWILFFQAIIFLGAVNA-DLNYKDALTKSLIFLEAQRSGKLPPN 60

Query: 77  HRPAWRGDSALDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAG 136
           HRPAWRGDSALDDGKLANVDL GGYYDAGDNVKYGLPMAFTVTTL+WAA+TY AE+EAAG
Sbjct: 61  HRPAWRGDSALDDGKLANVDLVGGYYDAGDNVKYGLPMAFTVTTLAWAALTYPAELEAAG 120

Query: 137 EMENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTP 196
           EMEN +AAI+WGTDYFLKA SRRNRLYV+VGDPVKDHECWVRPENM+TPRTVLQID+NTP
Sbjct: 121 EMENTKAAIQWGTDYFLKAASRRNRLYVEVGDPVKDHECWVRPENMRTPRTVLQIDSNTP 180

Query: 197 GTEIAAETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSG 256
           GTEIAAETSAAMASSS+VFRS N+TYARRLLNKAKLLFQFAK HKGTYDGECPFYCS+SG
Sbjct: 181 GTEIAAETSAAMASSSMVFRSCNRTYARRLLNKAKLLFQFAKNHKGTYDGECPFYCSYSG 240

Query: 257 YNDELLWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKVNFIKLQQ 316
           +NDELLWAATWLYIAT++PVYLKY++EE+I+ SVAEFSWDLKYAGAQILLSK  F    +
Sbjct: 241 FNDELLWAATWLYIATRKPVYLKYIQEESISASVAEFSWDLKYAGAQILLSKFYF----E 300

Query: 317 HQKGLQTLKDQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDI 376
            +K LQ  K+QADSYICSNLPNSPYHQIY+SPGGM+HMRDGANTQYVTGTAFLFSAYSD+
Sbjct: 301 GEKELQMYKNQADSYICSNLPNSPYHQIYISPGGMVHMRDGANTQYVTGTAFLFSAYSDL 360

Query: 377 LATFKQTVKCADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASV 436
           LAT+KQTV+CA  QFDSTQLM+FAKKQMDYVLGINPLGRSYMVGFGNNPP QAHHRGASV
Sbjct: 361 LATYKQTVQCATVQFDSTQLMAFAKKQMDYVLGINPLGRSYMVGFGNNPPTQAHHRGASV 420

Query: 437 PVLAPNAEVSCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTN 496
           PVLAP+  VSCPMSFV+WFNKNTPNPNELTGAILGGPD+ DNFVDKRWDSPMTEPVTYTN
Sbjct: 421 PVLAPDVVVSCPMSFVDWFNKNTPNPNELTGAILGGPDRTDNFVDKRWDSPMTEPVTYTN 480

Query: 497 SLAVGVIAKLA 503
           SLAVGV+AKLA
Sbjct: 481 SLAVGVLAKLA 486

[Close]

BLAST of MS001389 vs. NCBI nr
Match: XP_023002656.1 (endoglucanase 16-like [Cucurbita maxima])

HSP 1 Score: 822.0 bits (2122), Expect = 2.8e-234
Identity = 398/489 (81.39%), Postives = 439/489 (89.78%), Query Frame = 0


Query: 17  MGALKMKLSTTAIVVGFLALFQTFL---VDATELNYKDALSKALIFLEAQRSGKLPPNHR 76
           MG +   LS  AI+VG L  FQ F    V A ELNYKDAL+K+LIFLEAQRSGKLPPNHR
Sbjct: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAAELNYKDALTKSLIFLEAQRSGKLPPNHR 60

Query: 77  PAWRGDSALDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEM 136
           PAWRGDSALDDGKLANVDL GGYYDAGDNVKYGLPMAFTVTTLSW A+TY  E+EAAGEM
Sbjct: 61  PAWRGDSALDDGKLANVDLVGGYYDAGDNVKYGLPMAFTVTTLSWGALTYPTELEAAGEM 120

Query: 137 ENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGT 196
           ENV+AAIKWGTDYFLKA S R+RLYV+VGDPVKDHECWVRPENMKTPRTVLQID+ TPGT
Sbjct: 121 ENVKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPENMKTPRTVLQIDSETPGT 180

Query: 197 EIAAETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYN 256
           EIAAETSAAMASSSIVFRS N+TYAR LLNKAK L++FAK+HKGTYDGECPFYCS+SGYN
Sbjct: 181 EIAAETSAAMASSSIVFRSSNQTYARLLLNKAKTLYKFAKSHKGTYDGECPFYCSYSGYN 240

Query: 257 DELLWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKVNFIKLQQHQ 316
           DELLWAATWLYIAT++ VYLKYV EE+I+ SVAEFSWDLKY GAQ+LLSK+ F    + +
Sbjct: 241 DELLWAATWLYIATRKSVYLKYVLEESISASVAEFSWDLKYVGAQVLLSKLYF----EGE 300

Query: 317 KGLQTLKDQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILA 376
           KGL+T K+QAD+YICSNLP SPYHQIYVSPGGM+HMRDGANTQYVTGTAF+FSAYSDILA
Sbjct: 301 KGLETFKNQADNYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSDILA 360

Query: 377 TFKQTVKCADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPV 436
            +KQ VKC+DQQFDST LM+FAKKQMDY+LG NPLGRS+MVGFGNNPP QAHHRGAS+PV
Sbjct: 361 AYKQNVKCSDQQFDSTHLMTFAKKQMDYLLGDNPLGRSFMVGFGNNPPTQAHHRGASMPV 420

Query: 437 LAPNAEVSCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSL 496
           +  NAEV+CPMSFV W NK+TPNPNELTGAILGGPD+ND F+DKR  SPMTEPVTYTNS+
Sbjct: 421 MPANAEVNCPMSFVNWLNKDTPNPNELTGAILGGPDRNDKFLDKRTVSPMTEPVTYTNSM 480

Query: 497 AVGVIAKLA 503
           AVGV+AKLA
Sbjct: 481 AVGVLAKLA 485

[Close]

BLAST of MS001389 vs. NCBI nr
Match: XP_023537900.1 (endoglucanase 16-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 815.5 bits (2105), Expect = 2.6e-232
Identity = 393/489 (80.37%), Postives = 437/489 (89.37%), Query Frame = 0


Query: 17  MGALKMKLSTTAIVVGFLALFQTFL---VDATELNYKDALSKALIFLEAQRSGKLPPNHR 76
           MG +   LS  AI+VG L  FQ F    V A E NYKDAL+K+LIFLEAQRSGKLPPNHR
Sbjct: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAAEFNYKDALTKSLIFLEAQRSGKLPPNHR 60

Query: 77  PAWRGDSALDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEM 136
           PAWRGDSALDDGKLANVDL GGYYDAGDNVKYGLPMAFTVTTLSW A+TY  E+EAAGEM
Sbjct: 61  PAWRGDSALDDGKLANVDLVGGYYDAGDNVKYGLPMAFTVTTLSWGALTYPTELEAAGEM 120

Query: 137 ENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGT 196
           EN++AAI+WGTDYFLKA S R+RLYV+VGDPVKDHECWVRPENMKTPRTVLQI++ TPGT
Sbjct: 121 ENLKAAIRWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPENMKTPRTVLQINSETPGT 180

Query: 197 EIAAETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYN 256
           EIAAETSAAMASSS+VFRS N+TYAR LLNKAK L++FAKAHKGTYDGECPFYCS+SGYN
Sbjct: 181 EIAAETSAAMASSSMVFRSSNQTYARLLLNKAKTLYKFAKAHKGTYDGECPFYCSYSGYN 240

Query: 257 DELLWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKVNFIKLQQHQ 316
           DELLWAATWLY+AT++ VYLKYV+EE+I+ SVAEFSWDLKY GAQ+LLSK+ F    + +
Sbjct: 241 DELLWAATWLYVATRKSVYLKYVQEESISASVAEFSWDLKYVGAQVLLSKLYF----EGE 300

Query: 317 KGLQTLKDQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILA 376
           KGL+T K+QADSYICSNLP SPYHQIYVSPGGM+HMRDGANTQYVTGTAF+FSAYSDILA
Sbjct: 301 KGLETFKNQADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSDILA 360

Query: 377 TFKQTVKCADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPV 436
            +KQ VKC+DQQFD   LM+FAKKQMDY+LG NPLGRS+MVGFGNNPP QAHHRGASVPV
Sbjct: 361 AYKQNVKCSDQQFDPAHLMTFAKKQMDYLLGDNPLGRSFMVGFGNNPPTQAHHRGASVPV 420

Query: 437 LAPNAEVSCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSL 496
           +  NAEV+CPMSFV W NK+TPNPNELTGAILGGPD+ND F+DKR  SPMTEPVTYTNS+
Sbjct: 421 MPANAEVNCPMSFVNWLNKDTPNPNELTGAILGGPDRNDKFLDKRTVSPMTEPVTYTNSM 480

Query: 497 AVGVIAKLA 503
           AVGV+AKLA
Sbjct: 481 AVGVLAKLA 485

[Close]

BLAST of MS001389 vs. NCBI nr
Match: XP_022951947.1 (endoglucanase 16-like [Cucurbita moschata])

HSP 1 Score: 814.7 bits (2103), Expect = 4.4e-232
Identity = 394/489 (80.57%), Postives = 435/489 (88.96%), Query Frame = 0


Query: 17  MGALKMKLSTTAIVVGFLALFQTFL---VDATELNYKDALSKALIFLEAQRSGKLPPNHR 76
           MG +   LS  AI+VG L  FQ F    V A + NYKDAL+K+LIFLEAQRSGKLPPNHR
Sbjct: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHR 60

Query: 77  PAWRGDSALDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEM 136
           PAWRGDSALDDGKLANVDL GGYYDAGDNVKYGLPMAFTVTTLSW A+TY AE+EAAGEM
Sbjct: 61  PAWRGDSALDDGKLANVDLVGGYYDAGDNVKYGLPMAFTVTTLSWGALTYPAELEAAGEM 120

Query: 137 ENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGT 196
           EN++AAIKWGTDYFLKA S R+RLYV+VGDPVKDHECWVRPENMKTPRTVLQID+ TPGT
Sbjct: 121 ENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPENMKTPRTVLQIDSETPGT 180

Query: 197 EIAAETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYN 256
           EIAAETSAAMASSSIVFR  N+TYAR LLNKAK L++FAKAHK TYDGECPFYCS+SGYN
Sbjct: 181 EIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHKATYDGECPFYCSYSGYN 240

Query: 257 DELLWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKVNFIKLQQHQ 316
           DELLWAATWLY+AT++ VYLKYV EE+I+ SVAEFSWDLKY GAQ+LLSK+ F    + +
Sbjct: 241 DELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVGAQVLLSKLYF----EGE 300

Query: 317 KGLQTLKDQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILA 376
           KGL+T K+QADSYICSNLP SPYHQIYVSPGGM+HMRDGANTQYVTGTAF+FSAYSDILA
Sbjct: 301 KGLETFKNQADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSDILA 360

Query: 377 TFKQTVKCADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPV 436
            +KQ VKC+DQQFD   LM+FAKKQMDY+LG NPLGRS+MVGFGNNPP QAHHRGASVPV
Sbjct: 361 AYKQNVKCSDQQFDPAHLMTFAKKQMDYLLGDNPLGRSFMVGFGNNPPTQAHHRGASVPV 420

Query: 437 LAPNAEVSCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSL 496
           +  NAEV+CPMSFV W NK+TPNPNELTGAILGGPD+ND F+DKR  SPMTEPVTYTNS+
Sbjct: 421 MPANAEVNCPMSFVNWLNKDTPNPNELTGAILGGPDRNDKFLDKRTVSPMTEPVTYTNSM 480

Query: 497 AVGVIAKLA 503
           AVGV+AKLA
Sbjct: 481 AVGVLAKLA 485

[Close]

BLAST of MS001389 vs. ExPASy Swiss-Prot
Match: Q8VYG3 (Endoglucanase 16 OS=Arabidopsis thaliana OX=3702 GN=At3g43860 PE=2 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 2.0e-203
Identity = 335/475 (70.53%), Postives = 391/475 (82.32%), Query Frame = 0


Query: 28  AIVVGFLALFQTFLVDATELNYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSALDDGKL 87
           ++++G   +     V+ T +NYKDAL+K+LIFLEAQRSGKLPPN+R  WRGDSALDDGKL
Sbjct: 14  SMLLGLYGIINIVCVNGTFINYKDALTKSLIFLEAQRSGKLPPNNRVPWRGDSALDDGKL 73

Query: 88  ANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTDYF 147
            NVDL GGYYDAGDNVKYGLPMAFT+TTL+W+ +TY+ E+ A GE+EN RAAI+WGTDYF
Sbjct: 74  VNVDLSGGYYDAGDNVKYGLPMAFTITTLAWSTITYEKELRATGELENARAAIRWGTDYF 133

Query: 148 LKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMASSS 207
           LK  SR+NRLYVQVGDP  DH+CW RPENMKTPRTVL+I +  PGTEIAAE +AA A+SS
Sbjct: 134 LKCASRKNRLYVQVGDPNADHQCWARPENMKTPRTVLEISDKVPGTEIAAEAAAAFAASS 193

Query: 208 IVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDELLWAATWLYIAT 267
           IVFR V+  YARRLLNKAKLLF+ AK+HKGTYDGECPFYCS SGYNDEL+WAATWLY AT
Sbjct: 194 IVFRHVDHKYARRLLNKAKLLFKLAKSHKGTYDGECPFYCSNSGYNDELIWAATWLYKAT 253

Query: 268 KRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKVNFIKLQQHQKGLQTLKDQADSYI 327
           +  +YL Y+K EAI+  VAEFSWDLKYAGAQIL++K+ F    +  KGL   K QADS++
Sbjct: 254 RNHLYLSYLKFEAISAYVAEFSWDLKYAGAQILITKLIF----EGHKGLDLYKQQADSFV 313

Query: 328 CSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQTVKCADQQFD 387
           CSNLP SPYHQ++ +PGGMIH+RDGAN+QYVT TAFLFSAY+DIL    Q + C   QFD
Sbjct: 314 CSNLPGSPYHQVFTTPGGMIHLRDGANSQYVTATAFLFSAYADILQKHNQKISCGSHQFD 373

Query: 388 STQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPNAEVSCPMSFV 447
           ST LM+FAKKQ+DY+LG NP GRSYMVGFG NPP QAHHRGASVP+   NA +SCP+SFV
Sbjct: 374 STHLMAFAKKQIDYILGHNPQGRSYMVGFGPNPPKQAHHRGASVPMHEANAPLSCPLSFV 433

Query: 448 EWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKLA 503
           +W+NKN PN NELTGAILGGPD+ D F D RW S  TEP TY NS+AVGV+AKLA
Sbjct: 434 KWYNKNVPNANELTGAILGGPDRQDKFQDLRWTSVYTEPCTYINSIAVGVLAKLA 484

[Close]

BLAST of MS001389 vs. ExPASy Swiss-Prot
Match: Q6K7G9 (Endoglucanase 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0778600 PE=2 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 1.3e-186
Identity = 306/459 (66.67%), Postives = 374/459 (81.48%), Query Frame = 0


Query: 44  ATELNYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSALDDGKLANVDLEGGYYDAGDNV 103
           A   NYKDAL+K+++FLEAQRSGKLPP +R  WRGDS ++DGKLANVDL GGYYDAGDNV
Sbjct: 38  APSFNYKDALTKSIMFLEAQRSGKLPPTNRIKWRGDSGMEDGKLANVDLTGGYYDAGDNV 97

Query: 104 KYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTDYFLKACSRRNRLYVQVGD 163
           KYGLP+AFTVTTL+W AM ++ E++AA E+ENV AAI+WGTDYFLKA ++++ L+VQVGD
Sbjct: 98  KYGLPLAFTVTTLAWTAMAFEKELKAARELENVHAAIRWGTDYFLKAATKKDHLWVQVGD 157

Query: 164 PVKDHECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMASSSIVFRSVNKTYARRLLN 223
           P  DH+CWVRPENM TPRT+ QI++ TPG+EIAAET+AAM +SS+VFR  +K Y+RRLLN
Sbjct: 158 PNADHQCWVRPENMPTPRTLYQINDKTPGSEIAAETAAAMTASSMVFRK-DKPYSRRLLN 217

Query: 224 KAKLLFQFAKAHKGTYDGECPFYCSFSGYNDELLWAATWLYIATKRPVYLKYVKEEAITG 283
           KAKLLFQFAK H+GTYDGECPFYCS+SGYNDELLWAATWLY+ATKR VY  ++  EAI+ 
Sbjct: 218 KAKLLFQFAKTHQGTYDGECPFYCSYSGYNDELLWAATWLYLATKRQVYADFIGHEAISS 277

Query: 284 SVAEFSWDLKYAGAQILLSKVNFIKLQQHQKGLQTLKDQADSYICSNLPNSPYHQIYVSP 343
           SVAEFSWDLK+ GAQ+LL+++N         GLQ+ K QAD+++C+ LP++P+HQ+ ++P
Sbjct: 278 SVAEFSWDLKFPGAQVLLAELNMTS----SGGLQSFKSQADNFVCAVLPDTPFHQVSITP 337

Query: 344 GGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQTVKCADQQFDSTQLMSFAKKQMDYVL 403
           GGMIH+RDGAN+QYVT TAFLF AYSDIL    Q V C  Q     +L+ FAK+Q+DY+L
Sbjct: 338 GGMIHLRDGANSQYVTSTAFLFVAYSDILRRINQPVMCGAQAVQPARLLQFAKQQIDYLL 397

Query: 404 GINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPNAEVSCPMSFVEWFNKNTPNPNELTGA 463
           G NP GRSY+VGFG NPP Q HHRGAS PVL P  +V+C MSF EWF  + PNPNELTGA
Sbjct: 398 GANPRGRSYVVGFGVNPPTQPHHRGASTPVLPPGYQVNCGMSFSEWFTPDRPNPNELTGA 457

Query: 464 ILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKLA 503
           I+GGPD  DNF DKR +S  TEP TY NSL++G +A LA
Sbjct: 458 IMGGPDGGDNFSDKRGNSSCTEPCTYINSLSIGPLAALA 491

[Close]

BLAST of MS001389 vs. ExPASy Swiss-Prot
Match: P22503 (Endoglucanase OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 451.1 bits (1159), Expect = 1.7e-125
Identity = 220/457 (48.14%), Postives = 304/457 (66.52%), Query Frame = 0


Query: 48  NYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSALDDGKLANVDLEGGYYDAGDNVKYGL 107
           +Y DAL+KA++F E QRSGKLP + R  WR DSAL DGKL NV+L GGYYDAGDNVK+G 
Sbjct: 40  DYADALAKAILFFEGQRSGKLPSSQRVKWREDSALSDGKLQNVNLMGGYYDAGDNVKFGW 99

Query: 108 PMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVKD 167
           PMAF+ + LSWAA+ Y++EI +  ++  +++AI+WG D+ L+A +    LY QVGD   D
Sbjct: 100 PMAFSTSLLSWAAVEYESEISSVNQLGYLQSAIRWGADFMLRAHTSPTTLYTQVGDGNAD 159

Query: 168 HECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMASSSIVFRSVNKTYARRLLNKAKL 227
           H CW RPE+M TPRTV +ID N+PGTE+AAE +AA++++SIVF+ ++  Y+  LL+ +K 
Sbjct: 160 HNCWERPEDMDTPRTVYKIDANSPGTEVAAEYAAALSAASIVFKKIDAKYSSTLLSHSKS 219

Query: 228 LFQFAKAHKGTYDGECPFYCSFSGYNDELLWAATWLYIATKRPVYLKY-VKEEAITGSVA 287
           LF FA  ++G+Y G CPFYCS+SGY DELLWAA WLY A+    YL Y +  +  + +V+
Sbjct: 220 LFDFADKNRGSYSGSCPFYCSYSGYQDELLWAAAWLYKASGESKYLSYIISNQGWSQTVS 279

Query: 288 EFSWDLKYAGAQILLSKVNFIKLQQHQKGLQTLKDQADSYICSNLPNSPYHQIYVSPGGM 347
           EFSWD K+ GAQ LL++    +    +K L  +K  A+S+IC+ +P S   QI  +PGG+
Sbjct: 280 EFSWDNKFVGAQTLLTE----EFYGGKKDLAKIKTDAESFICAVMPGSNSRQIKTTPGGL 339

Query: 348 IHMRDGANTQYVTGTAFLFSAYSDIL-ATFKQTVKCADQQFDSTQLMSFAKKQMDYVLGI 407
           +  RD +N QY T +  +   +S IL       + C    F ++Q+  FAK Q++Y+LG 
Sbjct: 340 LFTRDSSNLQYTTSSTMVLFIFSRILNRNHINGINCGSSHFTASQIRGFAKTQVEYILGK 399

Query: 408 NPLGRSYMVGFGNNPPVQAHHRGASVPVLAPN-AEVSCPMSFVEWFNKNTPNPNELTGAI 467
           NP+  SYMVGFG+  P Q HHRG+S+P +  + A+V C     +++N   PNPN   GAI
Sbjct: 400 NPMKMSYMVGFGSKYPKQLHHRGSSIPSIKVHPAKVGCNAGLSDYYNSANPNPNTHVGAI 459

Query: 468 LGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKL 502
           +GGPD ND F D R D    EP TY N+  V  I+ L
Sbjct: 460 VGGPDSNDRFNDARSDYSHAEPTTYINAAFVASISAL 492

[Close]

BLAST of MS001389 vs. ExPASy Swiss-Prot
Match: Q9SZ90 (Endoglucanase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g09740 PE=3 SV=2)

HSP 1 Score: 446.0 bits (1146), Expect = 5.5e-124
Identity = 226/476 (47.48%), Postives = 306/476 (64.29%), Query Frame = 0


Query: 29  IVVGFLALFQTFLVDATELNYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSALDDGKLA 88
           +VV  + +F  F      L+Y DAL+K+++F E QRSGKLP N R  WR DS L DG  A
Sbjct: 5   LVVMLIGMFLAF-ESLEALDYGDALNKSILFFEGQRSGKLPTNQRVKWRADSGLSDGASA 64

Query: 89  NVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTDYFL 148
           NV+L GGYYDAGDNVK+  PM+FT T LSWAA+ YQ EI    ++  +R+ IKWGT++ L
Sbjct: 65  NVNLIGGYYDAGDNVKFVWPMSFTTTLLSWAALEYQNEITFVNQLGYLRSTIKWGTNFIL 124

Query: 149 KACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMASSSI 208
           +A +  N LY QVGD   DH CW RPE+M TPRT+  I +++PG+E A E +AA+A++S+
Sbjct: 125 RAHTSTNMLYTQVGDGNSDHSCWERPEDMDTPRTLYSISSSSPGSEAAGEAAAALAAASL 184

Query: 209 VFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDELLWAATWLYIATK 268
           VF+ V+ TY+ +LLN AK LF+FA  ++G+Y   CPFYCS SGY DELLWAA WLY AT 
Sbjct: 185 VFKLVDSTYSSKLLNNAKSLFEFADKYRGSYQASCPFYCSHSGYQDELLWAAAWLYKATG 244

Query: 269 RPVYLKYV-KEEAITGSVAEFSWDLKYAGAQILLSKVNFIKLQQHQKGLQTLKDQADSYI 328
              YL YV   +  + ++ EFSWD K+AG Q LL+     +       L+  K   +S++
Sbjct: 245 EKSYLNYVISNKDWSKAINEFSWDNKFAGVQALLAS----EFYNGANDLEKFKTDVESFV 304

Query: 329 CSNLPNSPYHQIYVSPGGMIHMRDGANTQYV-TGTAFLFSAYSDILATFKQTVKCADQQF 388
           C+ +P S   QI  +PGG++ +RD +N QYV T T  LF     +      +++C   QF
Sbjct: 305 CALMPGSSSQQIKPTPGGILFIRDSSNLQYVTTATTILFYYSKTLTKAGVGSIQCGSTQF 364

Query: 389 DSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPNAE-VSCPMS 448
             +Q+ +FAK Q+DY+LG NPL  SYMVGFG   P Q HHRG+S+P +    E + C   
Sbjct: 365 TVSQIRNFAKSQVDYILGNNPLKMSYMVGFGTKYPTQPHHRGSSLPSIQSKPEKIDCNGG 424

Query: 449 FVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKL 502
           F  ++N +TPNPN  TGAI+GGP+ +D + DKR D    EP TY N+  +G +A L
Sbjct: 425 F-SYYNFDTPNPNVHTGAIVGGPNSSDQYSDKRTDYSHAEPTTYINAAFIGSVAAL 474

[Close]

BLAST of MS001389 vs. ExPASy Swiss-Prot
Match: O81416 (Endoglucanase 17 OS=Arabidopsis thaliana OX=3702 GN=At4g02290 PE=2 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 9.3e-124
Identity = 224/465 (48.17%), Postives = 304/465 (65.38%), Query Frame = 0


Query: 48  NYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSALDDGKLANVDLEGGYYDAGDNVKYGL 107
           NYKDAL+K+++F E QRSGKLP N R +WR DS L DG   +VDL GGYYDAGDN+K+G 
Sbjct: 52  NYKDALTKSILFFEGQRSGKLPSNQRMSWRRDSGLSDGSALHVDLVGGYYDAGDNIKFGF 111

Query: 108 PMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVKD 167
           PMAFT T LSW+ + +   +++  E++N + AI+W TDY LKA S+ + +YVQVGD  KD
Sbjct: 112 PMAFTTTMLSWSVIEFGGLMKS--ELQNAKIAIRWATDYLLKATSQPDTIYVQVGDANKD 171

Query: 168 HECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMASSSIVFRSVNKTYARRLLNKAKL 227
           H CW RPE+M T R+V ++D N PG+++AAET+AA+A+++IVFR  + +Y++ LL +A  
Sbjct: 172 HSCWERPEDMDTVRSVFKVDKNIPGSDVAAETAAALAAAAIVFRKSDPSYSKVLLKRAIS 231

Query: 228 LFQFAKAHKGTYDG-----ECPFYCSFSGYNDELLWAATWLYIATKRPVYLKYVKEEAIT 287
           +F FA  ++GTY        CPFYCS+SGY DELLW A WL  ATK   YL Y+K     
Sbjct: 232 VFAFADKYRGTYSAGLKPDVCPFYCSYSGYQDELLWGAAWLQKATKNIKYLNYIKINGQI 291

Query: 288 GSVAE----FSWDLKYAGAQILLSKVNFIKLQQHQKGLQTLKDQADSYICSNLPNSPYHQ 347
              AE    F WD K+AGA+ILL+K   +   Q+ K L   K  AD++ICS +P +P+  
Sbjct: 292 LGAAEYDNTFGWDNKHAGARILLTKAFLV---QNVKTLHEYKGHADNFICSVIPGAPFSS 351

Query: 348 IYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQTVKCADQQFDSTQLMSFAKKQ 407
              +PGG++     AN QYVT T+FL   Y+  L + K  V C    +   +L S AK+Q
Sbjct: 352 TQYTPGGLLFKMADANMQYVTSTSFLLLTYAKYLTSAKTVVHCGGSVYTPGRLRSIAKRQ 411

Query: 408 MDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPN-AEVSCPMSFVEWFNKNTPNP 467
           +DY+LG NPL  SYMVG+G   P + HHRG+S+P +A + A++ C   F    N  +PNP
Sbjct: 412 VDYLLGDNPLRMSYMVGYGPKFPRRIHHRGSSLPCVASHPAKIQCHQGFA-IMNSQSPNP 471

Query: 468 NELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKLA 503
           N L GA++GGPD++D F D+R D   +EP TY NS  VG +A  A
Sbjct: 472 NFLVGAVVGGPDQHDRFPDERSDYEQSEPATYINSPLVGALAYFA 510

[Close]

BLAST of MS001389 vs. ExPASy TrEMBL
Match: A0A6J1BR89 (Endoglucanase OS=Momordica charantia OX=3673 GN=LOC111004858 PE=3 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.2e-277
Identity = 475/486 (97.74%), Postives = 478/486 (98.35%), Query Frame = 0


Query: 17  MGALKMKLSTTAIVVGFLALFQTFLVDATELNYKDALSKALIFLEAQRSGKLPPNHRPAW 76
           MGALKMKLSTTAI VGFLALFQTFLVDATELNYKDALSKALIFLEAQRSGKLPPNHRPAW
Sbjct: 1   MGALKMKLSTTAIAVGFLALFQTFLVDATELNYKDALSKALIFLEAQRSGKLPPNHRPAW 60

Query: 77  RGDSALDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENV 136
           RGDSA+DDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENV
Sbjct: 61  RGDSAVDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENV 120

Query: 137 RAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIA 196
           RAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIA
Sbjct: 121 RAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIA 180

Query: 197 AETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDEL 256
           AETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDEL
Sbjct: 181 AETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDEL 240

Query: 257 LWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKVNFIKLQQHQKGL 316
           LWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSK  F    + +KGL
Sbjct: 241 LWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKFYF----EGEKGL 300

Query: 317 QTLKDQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFK 376
           QTLKDQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFK
Sbjct: 301 QTLKDQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFK 360

Query: 377 QTVKCADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAP 436
           QTVKCADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAP
Sbjct: 361 QTVKCADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAP 420

Query: 437 NAEVSCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVG 496
           NAEVSCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVG
Sbjct: 421 NAEVSCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVG 480

Query: 497 VIAKLA 503
           VIAKLA
Sbjct: 481 VIAKLA 482

[Close]

BLAST of MS001389 vs. ExPASy TrEMBL
Match: A0A6J1KLX1 (Endoglucanase OS=Cucurbita maxima OX=3661 GN=LOC111496447 PE=3 SV=1)

HSP 1 Score: 822.0 bits (2122), Expect = 1.3e-234
Identity = 398/489 (81.39%), Postives = 439/489 (89.78%), Query Frame = 0


Query: 17  MGALKMKLSTTAIVVGFLALFQTFL---VDATELNYKDALSKALIFLEAQRSGKLPPNHR 76
           MG +   LS  AI+VG L  FQ F    V A ELNYKDAL+K+LIFLEAQRSGKLPPNHR
Sbjct: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAAELNYKDALTKSLIFLEAQRSGKLPPNHR 60

Query: 77  PAWRGDSALDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEM 136
           PAWRGDSALDDGKLANVDL GGYYDAGDNVKYGLPMAFTVTTLSW A+TY  E+EAAGEM
Sbjct: 61  PAWRGDSALDDGKLANVDLVGGYYDAGDNVKYGLPMAFTVTTLSWGALTYPTELEAAGEM 120

Query: 137 ENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGT 196
           ENV+AAIKWGTDYFLKA S R+RLYV+VGDPVKDHECWVRPENMKTPRTVLQID+ TPGT
Sbjct: 121 ENVKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPENMKTPRTVLQIDSETPGT 180

Query: 197 EIAAETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYN 256
           EIAAETSAAMASSSIVFRS N+TYAR LLNKAK L++FAK+HKGTYDGECPFYCS+SGYN
Sbjct: 181 EIAAETSAAMASSSIVFRSSNQTYARLLLNKAKTLYKFAKSHKGTYDGECPFYCSYSGYN 240

Query: 257 DELLWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKVNFIKLQQHQ 316
           DELLWAATWLYIAT++ VYLKYV EE+I+ SVAEFSWDLKY GAQ+LLSK+ F    + +
Sbjct: 241 DELLWAATWLYIATRKSVYLKYVLEESISASVAEFSWDLKYVGAQVLLSKLYF----EGE 300

Query: 317 KGLQTLKDQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILA 376
           KGL+T K+QAD+YICSNLP SPYHQIYVSPGGM+HMRDGANTQYVTGTAF+FSAYSDILA
Sbjct: 301 KGLETFKNQADNYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSDILA 360

Query: 377 TFKQTVKCADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPV 436
            +KQ VKC+DQQFDST LM+FAKKQMDY+LG NPLGRS+MVGFGNNPP QAHHRGAS+PV
Sbjct: 361 AYKQNVKCSDQQFDSTHLMTFAKKQMDYLLGDNPLGRSFMVGFGNNPPTQAHHRGASMPV 420

Query: 437 LAPNAEVSCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSL 496
           +  NAEV+CPMSFV W NK+TPNPNELTGAILGGPD+ND F+DKR  SPMTEPVTYTNS+
Sbjct: 421 MPANAEVNCPMSFVNWLNKDTPNPNELTGAILGGPDRNDKFLDKRTVSPMTEPVTYTNSM 480

Query: 497 AVGVIAKLA 503
           AVGV+AKLA
Sbjct: 481 AVGVLAKLA 485

[Close]

BLAST of MS001389 vs. ExPASy TrEMBL
Match: A0A6J1GJ20 (Endoglucanase OS=Cucurbita moschata OX=3662 GN=LOC111454680 PE=3 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 2.2e-232
Identity = 394/489 (80.57%), Postives = 435/489 (88.96%), Query Frame = 0


Query: 17  MGALKMKLSTTAIVVGFLALFQTFL---VDATELNYKDALSKALIFLEAQRSGKLPPNHR 76
           MG +   LS  AI+VG L  FQ F    V A + NYKDAL+K+LIFLEAQRSGKLPPNHR
Sbjct: 1   MGTIIHNLSAMAILVGSLLFFQPFFPGAVHAADFNYKDALTKSLIFLEAQRSGKLPPNHR 60

Query: 77  PAWRGDSALDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEM 136
           PAWRGDSALDDGKLANVDL GGYYDAGDNVKYGLPMAFTVTTLSW A+TY AE+EAAGEM
Sbjct: 61  PAWRGDSALDDGKLANVDLVGGYYDAGDNVKYGLPMAFTVTTLSWGALTYPAELEAAGEM 120

Query: 137 ENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGT 196
           EN++AAIKWGTDYFLKA S R+RLYV+VGDPVKDHECWVRPENMKTPRTVLQID+ TPGT
Sbjct: 121 ENLKAAIKWGTDYFLKASSHRDRLYVEVGDPVKDHECWVRPENMKTPRTVLQIDSETPGT 180

Query: 197 EIAAETSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYN 256
           EIAAETSAAMASSSIVFR  N+TYAR LLNKAK L++FAKAHK TYDGECPFYCS+SGYN
Sbjct: 181 EIAAETSAAMASSSIVFRHSNQTYARLLLNKAKTLYKFAKAHKATYDGECPFYCSYSGYN 240

Query: 257 DELLWAATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKVNFIKLQQHQ 316
           DELLWAATWLY+AT++ VYLKYV EE+I+ SVAEFSWDLKY GAQ+LLSK+ F    + +
Sbjct: 241 DELLWAATWLYVATRKSVYLKYVLEESISASVAEFSWDLKYVGAQVLLSKLYF----EGE 300

Query: 317 KGLQTLKDQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILA 376
           KGL+T K+QADSYICSNLP SPYHQIYVSPGGM+HMRDGANTQYVTGTAF+FSAYSDILA
Sbjct: 301 KGLETFKNQADSYICSNLPTSPYHQIYVSPGGMVHMRDGANTQYVTGTAFVFSAYSDILA 360

Query: 377 TFKQTVKCADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPV 436
            +KQ VKC+DQQFD   LM+FAKKQMDY+LG NPLGRS+MVGFGNNPP QAHHRGASVPV
Sbjct: 361 AYKQNVKCSDQQFDPAHLMTFAKKQMDYLLGDNPLGRSFMVGFGNNPPTQAHHRGASVPV 420

Query: 437 LAPNAEVSCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSL 496
           +  NAEV+CPMSFV W NK+TPNPNELTGAILGGPD+ND F+DKR  SPMTEPVTYTNS+
Sbjct: 421 MPANAEVNCPMSFVNWLNKDTPNPNELTGAILGGPDRNDKFLDKRTVSPMTEPVTYTNSM 480

Query: 497 AVGVIAKLA 503
           AVGV+AKLA
Sbjct: 481 AVGVLAKLA 485

[Close]

BLAST of MS001389 vs. ExPASy TrEMBL
Match: A0A1S3BDP2 (Endoglucanase OS=Cucumis melo OX=3656 GN=LOC103488526 PE=3 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 5.3e-231
Identity = 394/484 (81.40%), Postives = 437/484 (90.29%), Query Frame = 0


Query: 24  LSTTAI--VVGFLALFQTFL---VDATELNYKDALSKALIFLEAQRSGKLPPNHRPAWRG 83
           +STT I  +V  L  FQ+ +   V   +LNYKDAL+K+LIFLEAQRSGKLPPNHRPAWRG
Sbjct: 9   VSTTPILLLVYCLVFFQSIVFLRVVNADLNYKDALTKSLIFLEAQRSGKLPPNHRPAWRG 68

Query: 84  DSALDDGKLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENVRA 143
           DSALDDGKLANVDL GGYYDAGDNVKYGLPMAFT+TTL+W A+TY  EI+AAGEMENV+A
Sbjct: 69  DSALDDGKLANVDLVGGYYDAGDNVKYGLPMAFTITTLAWGALTYPEEIKAAGEMENVKA 128

Query: 144 AIKWGTDYFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIAAE 203
           A++WGTDYFLKA SRRNRLYVQVGDPVKDHECWVRPENMKT RTVLQID+NTPGTEIAAE
Sbjct: 129 ALQWGTDYFLKAASRRNRLYVQVGDPVKDHECWVRPENMKTERTVLQIDSNTPGTEIAAE 188

Query: 204 TSAAMASSSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDELLW 263
           TSAAMAS+S+VFRS N+TYARRLLNKAKLL+Q AK HKGTYDGECPFYCS+SGYNDELLW
Sbjct: 189 TSAAMASASMVFRSSNRTYARRLLNKAKLLYQLAKNHKGTYDGECPFYCSYSGYNDELLW 248

Query: 264 AATWLYIATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKVNFIKLQQHQKGLQT 323
           AATWLYIAT+RPVYLKYV+EE+I+ SVAEFSWDLKYAGAQILLSK  F    + +KGLQ 
Sbjct: 249 AATWLYIATRRPVYLKYVQEESISASVAEFSWDLKYAGAQILLSKFYF----EGEKGLQM 308

Query: 324 LKDQADSYICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQT 383
            K+QADSYICSNLP SP+HQI++SPGGM+H+RDGANTQYV GTAFLFSAYSD+LAT+KQT
Sbjct: 309 YKNQADSYICSNLPESPFHQIFISPGGMVHLRDGANTQYVAGTAFLFSAYSDLLATYKQT 368

Query: 384 VKCADQQFDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPNA 443
            +CAD+ FDS QLM FAKKQMDY+LGINPLGRS+MVGFGNNPP QAHHRGASVPVL PN 
Sbjct: 369 AQCADKSFDSKQLMMFAKKQMDYMLGINPLGRSFMVGFGNNPPKQAHHRGASVPVLPPNT 428

Query: 444 EVSCPMSFVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVI 503
            V+CPMSFV+WFNK+TPNPNELTGAILGGPD+ D F DKR DSPMTEPVTYTNSLAVGV+
Sbjct: 429 VVNCPMSFVDWFNKDTPNPNELTGAILGGPDRTDKFNDKRSDSPMTEPVTYTNSLAVGVL 488

[Close]

BLAST of MS001389 vs. ExPASy TrEMBL
Match: A0A6J1KB06 (Endoglucanase OS=Cucurbita maxima OX=3661 GN=LOC111492677 PE=3 SV=1)

HSP 1 Score: 805.8 bits (2080), Expect = 1.0e-229
Identity = 389/477 (81.55%), Postives = 430/477 (90.15%), Query Frame = 0


Query: 29  IVVGFLALFQTFL---VDATELNYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSALDDG 88
           I++  L LFQ      + A + NYKDAL+K LIFLEAQRSGKLPPNHRP WRGDSALDDG
Sbjct: 3   ILLPSLLLFQALFLAPLHAAQFNYKDALTKCLIFLEAQRSGKLPPNHRPPWRGDSALDDG 62

Query: 89  KLANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTD 148
           KLANVDLEGGYYDAGDNVKYGLPMAFTVTTL+WAA+ Y AE +AAGEMEN++AAI+WGTD
Sbjct: 63  KLANVDLEGGYYDAGDNVKYGLPMAFTVTTLAWAALAYPAETQAAGEMENLKAAIQWGTD 122

Query: 149 YFLKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMAS 208
           Y LKA SRRNR YV+VGDPVKDHECW+RPENMKTPRTVLQID  TPGTEIAAETSAAMAS
Sbjct: 123 YLLKASSRRNRFYVEVGDPVKDHECWIRPENMKTPRTVLQIDRTTPGTEIAAETSAAMAS 182

Query: 209 SSIVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDELLWAATWLYI 268
           SSIVFR+ N+TY+RRLLNKAKLLFQFAK ++GTYDGECPFYCS+SGYNDELLWAATWLYI
Sbjct: 183 SSIVFRNSNRTYSRRLLNKAKLLFQFAKMNRGTYDGECPFYCSYSGYNDELLWAATWLYI 242

Query: 269 ATKRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKVNFIKLQQHQKGLQTLKDQADS 328
           AT+RP YLKY+ EE+++GSVAEFSWDLKYAGAQILLS++ F    Q +KGLQ  K+QADS
Sbjct: 243 ATRRPAYLKYITEESVSGSVAEFSWDLKYAGAQILLSEIYF----QGEKGLQMYKNQADS 302

Query: 329 YICSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQTVKCADQQ 388
           YICSNLPNSPYHQIY+SPGGM+HMRDGANTQYVTGTAFLFSAYSDILAT +QTV+C  QQ
Sbjct: 303 YICSNLPNSPYHQIYLSPGGMVHMRDGANTQYVTGTAFLFSAYSDILATHQQTVQCGGQQ 362

Query: 389 FDSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPNAEVSCPMS 448
           FDSTQLM+FAKKQMDY+LG NP GRSYMVGFGNNPP QAHHRGASVPV+  NAEV+C MS
Sbjct: 363 FDSTQLMTFAKKQMDYLLGNNPQGRSYMVGFGNNPPTQAHHRGASVPVMPVNAEVNCAMS 422

Query: 449 FVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKLA 503
           FV W NK+TPNPNELTGAILGGPD++DNF+DKR  SPMTEPVTYTNSLAVGV+AKLA
Sbjct: 423 FVYWLNKDTPNPNELTGAILGGPDRSDNFLDKRVVSPMTEPVTYTNSLAVGVLAKLA 475

[Close]

BLAST of MS001389 vs. TAIR 10
Match: AT3G43860.1 (glycosyl hydrolase 9A4 )

HSP 1 Score: 709.9 bits (1831), Expect = 1.4e-204
Identity = 335/475 (70.53%), Postives = 391/475 (82.32%), Query Frame = 0


Query: 28  AIVVGFLALFQTFLVDATELNYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSALDDGKL 87
           ++++G   +     V+ T +NYKDAL+K+LIFLEAQRSGKLPPN+R  WRGDSALDDGKL
Sbjct: 14  SMLLGLYGIINIVCVNGTFINYKDALTKSLIFLEAQRSGKLPPNNRVPWRGDSALDDGKL 73

Query: 88  ANVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTDYF 147
            NVDL GGYYDAGDNVKYGLPMAFT+TTL+W+ +TY+ E+ A GE+EN RAAI+WGTDYF
Sbjct: 74  VNVDLSGGYYDAGDNVKYGLPMAFTITTLAWSTITYEKELRATGELENARAAIRWGTDYF 133

Query: 148 LKACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMASSS 207
           LK  SR+NRLYVQVGDP  DH+CW RPENMKTPRTVL+I +  PGTEIAAE +AA A+SS
Sbjct: 134 LKCASRKNRLYVQVGDPNADHQCWARPENMKTPRTVLEISDKVPGTEIAAEAAAAFAASS 193

Query: 208 IVFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDELLWAATWLYIAT 267
           IVFR V+  YARRLLNKAKLLF+ AK+HKGTYDGECPFYCS SGYNDEL+WAATWLY AT
Sbjct: 194 IVFRHVDHKYARRLLNKAKLLFKLAKSHKGTYDGECPFYCSNSGYNDELIWAATWLYKAT 253

Query: 268 KRPVYLKYVKEEAITGSVAEFSWDLKYAGAQILLSKVNFIKLQQHQKGLQTLKDQADSYI 327
           +  +YL Y+K EAI+  VAEFSWDLKYAGAQIL++K+ F    +  KGL   K QADS++
Sbjct: 254 RNHLYLSYLKFEAISAYVAEFSWDLKYAGAQILITKLIF----EGHKGLDLYKQQADSFV 313

Query: 328 CSNLPNSPYHQIYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQTVKCADQQFD 387
           CSNLP SPYHQ++ +PGGMIH+RDGAN+QYVT TAFLFSAY+DIL    Q + C   QFD
Sbjct: 314 CSNLPGSPYHQVFTTPGGMIHLRDGANSQYVTATAFLFSAYADILQKHNQKISCGSHQFD 373

Query: 388 STQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPNAEVSCPMSFV 447
           ST LM+FAKKQ+DY+LG NP GRSYMVGFG NPP QAHHRGASVP+   NA +SCP+SFV
Sbjct: 374 STHLMAFAKKQIDYILGHNPQGRSYMVGFGPNPPKQAHHRGASVPMHEANAPLSCPLSFV 433

Query: 448 EWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKLA 503
           +W+NKN PN NELTGAILGGPD+ D F D RW S  TEP TY NS+AVGV+AKLA
Sbjct: 434 KWYNKNVPNANELTGAILGGPDRQDKFQDLRWTSVYTEPCTYINSIAVGVLAKLA 484

[Close]

BLAST of MS001389 vs. TAIR 10
Match: AT4G09740.1 (glycosyl hydrolase 9B14 )

HSP 1 Score: 446.0 bits (1146), Expect = 3.9e-125
Identity = 226/476 (47.48%), Postives = 306/476 (64.29%), Query Frame = 0


Query: 29  IVVGFLALFQTFLVDATELNYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSALDDGKLA 88
           +VV  + +F  F      L+Y DAL+K+++F E QRSGKLP N R  WR DS L DG  A
Sbjct: 5   LVVMLIGMFLAF-ESLEALDYGDALNKSILFFEGQRSGKLPTNQRVKWRADSGLSDGASA 64

Query: 89  NVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTDYFL 148
           NV+L GGYYDAGDNVK+  PM+FT T LSWAA+ YQ EI    ++  +R+ IKWGT++ L
Sbjct: 65  NVNLIGGYYDAGDNVKFVWPMSFTTTLLSWAALEYQNEITFVNQLGYLRSTIKWGTNFIL 124

Query: 149 KACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMASSSI 208
           +A +  N LY QVGD   DH CW RPE+M TPRT+  I +++PG+E A E +AA+A++S+
Sbjct: 125 RAHTSTNMLYTQVGDGNSDHSCWERPEDMDTPRTLYSISSSSPGSEAAGEAAAALAAASL 184

Query: 209 VFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDELLWAATWLYIATK 268
           VF+ V+ TY+ +LLN AK LF+FA  ++G+Y   CPFYCS SGY DELLWAA WLY AT 
Sbjct: 185 VFKLVDSTYSSKLLNNAKSLFEFADKYRGSYQASCPFYCSHSGYQDELLWAAAWLYKATG 244

Query: 269 RPVYLKYV-KEEAITGSVAEFSWDLKYAGAQILLSKVNFIKLQQHQKGLQTLKDQADSYI 328
              YL YV   +  + ++ EFSWD K+AG Q LL+     +       L+  K   +S++
Sbjct: 245 EKSYLNYVISNKDWSKAINEFSWDNKFAGVQALLAS----EFYNGANDLEKFKTDVESFV 304

Query: 329 CSNLPNSPYHQIYVSPGGMIHMRDGANTQYV-TGTAFLFSAYSDILATFKQTVKCADQQF 388
           C+ +P S   QI  +PGG++ +RD +N QYV T T  LF     +      +++C   QF
Sbjct: 305 CALMPGSSSQQIKPTPGGILFIRDSSNLQYVTTATTILFYYSKTLTKAGVGSIQCGSTQF 364

Query: 389 DSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPNAE-VSCPMS 448
             +Q+ +FAK Q+DY+LG NPL  SYMVGFG   P Q HHRG+S+P +    E + C   
Sbjct: 365 TVSQIRNFAKSQVDYILGNNPLKMSYMVGFGTKYPTQPHHRGSSLPSIQSKPEKIDCNGG 424

Query: 449 FVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKL 502
           F  ++N +TPNPN  TGAI+GGP+ +D + DKR D    EP TY N+  +G +A L
Sbjct: 425 F-SYYNFDTPNPNVHTGAIVGGPNSSDQYSDKRTDYSHAEPTTYINAAFIGSVAAL 474

[Close]

BLAST of MS001389 vs. TAIR 10
Match: AT4G02290.1 (glycosyl hydrolase 9B13 )

HSP 1 Score: 445.3 bits (1144), Expect = 6.6e-125
Identity = 224/465 (48.17%), Postives = 304/465 (65.38%), Query Frame = 0


Query: 48  NYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSALDDGKLANVDLEGGYYDAGDNVKYGL 107
           NYKDAL+K+++F E QRSGKLP N R +WR DS L DG   +VDL GGYYDAGDN+K+G 
Sbjct: 52  NYKDALTKSILFFEGQRSGKLPSNQRMSWRRDSGLSDGSALHVDLVGGYYDAGDNIKFGF 111

Query: 108 PMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVKD 167
           PMAFT T LSW+ + +   +++  E++N + AI+W TDY LKA S+ + +YVQVGD  KD
Sbjct: 112 PMAFTTTMLSWSVIEFGGLMKS--ELQNAKIAIRWATDYLLKATSQPDTIYVQVGDANKD 171

Query: 168 HECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMASSSIVFRSVNKTYARRLLNKAKL 227
           H CW RPE+M T R+V ++D N PG+++AAET+AA+A+++IVFR  + +Y++ LL +A  
Sbjct: 172 HSCWERPEDMDTVRSVFKVDKNIPGSDVAAETAAALAAAAIVFRKSDPSYSKVLLKRAIS 231

Query: 228 LFQFAKAHKGTYDG-----ECPFYCSFSGYNDELLWAATWLYIATKRPVYLKYVKEEAIT 287
           +F FA  ++GTY        CPFYCS+SGY DELLW A WL  ATK   YL Y+K     
Sbjct: 232 VFAFADKYRGTYSAGLKPDVCPFYCSYSGYQDELLWGAAWLQKATKNIKYLNYIKINGQI 291

Query: 288 GSVAE----FSWDLKYAGAQILLSKVNFIKLQQHQKGLQTLKDQADSYICSNLPNSPYHQ 347
              AE    F WD K+AGA+ILL+K   +   Q+ K L   K  AD++ICS +P +P+  
Sbjct: 292 LGAAEYDNTFGWDNKHAGARILLTKAFLV---QNVKTLHEYKGHADNFICSVIPGAPFSS 351

Query: 348 IYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQTVKCADQQFDSTQLMSFAKKQ 407
              +PGG++     AN QYVT T+FL   Y+  L + K  V C    +   +L S AK+Q
Sbjct: 352 TQYTPGGLLFKMADANMQYVTSTSFLLLTYAKYLTSAKTVVHCGGSVYTPGRLRSIAKRQ 411

Query: 408 MDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPN-AEVSCPMSFVEWFNKNTPNP 467
           +DY+LG NPL  SYMVG+G   P + HHRG+S+P +A + A++ C   F    N  +PNP
Sbjct: 412 VDYLLGDNPLRMSYMVGYGPKFPRRIHHRGSSLPCVASHPAKIQCHQGFA-IMNSQSPNP 471

Query: 468 NELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKLA 503
           N L GA++GGPD++D F D+R D   +EP TY NS  VG +A  A
Sbjct: 472 NFLVGAVVGGPDQHDRFPDERSDYEQSEPATYINSPLVGALAYFA 510

[Close]

BLAST of MS001389 vs. TAIR 10
Match: AT4G23560.1 (glycosyl hydrolase 9B15 )

HSP 1 Score: 444.9 bits (1143), Expect = 8.6e-125
Identity = 223/476 (46.85%), Postives = 306/476 (64.29%), Query Frame = 0


Query: 29  IVVGFLALFQTFLVDATELNYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSALDDGKLA 88
           ++VG    F++       L Y DAL+K+++F E QRSGKLP N R  WR DSAL DG LA
Sbjct: 8   MLVGMFLAFESL----EALEYGDALNKSILFFEGQRSGKLPTNQRVKWRADSALSDGSLA 67

Query: 89  NVDLEGGYYDAGDNVKYGLPMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTDYFL 148
           NV+L GGYYDAGDNVK+  PM+FT T LSWAA+ YQ EI +  ++  +R+ IKWGTD+ L
Sbjct: 68  NVNLIGGYYDAGDNVKFVWPMSFTTTLLSWAAIEYQNEISSVNQLGYLRSTIKWGTDFIL 127

Query: 149 KACSRRNRLYVQVGDPVKDHECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMASSSI 208
           +A +  N LY QVGD   DH CW RPE+M T RT+  I +++PG+E A E +AA+A++S+
Sbjct: 128 RAHTSPNMLYTQVGDGNSDHSCWERPEDMDTSRTLYSISSSSPGSEAAGEAAAALAAASL 187

Query: 209 VFRSVNKTYARRLLNKAKLLFQFAKAHKGTYDGECPFYCSFSGYNDELLWAATWLYIATK 268
           VF+SV+ TY+  LLN AK LF+FA  ++G+Y   CPFYCS+SGY DELLWAA WLY AT 
Sbjct: 188 VFKSVDSTYSSTLLNHAKTLFEFADKYRGSYQASCPFYCSYSGYQDELLWAAAWLYKATG 247

Query: 269 RPVYLKYV-KEEAITGSVAEFSWDLKYAGAQILLSKVNFIKLQQHQKGLQTLKDQADSYI 328
             +Y+ YV   +  + +V EFSWD K+ GAQ LL      +       L   K   +S++
Sbjct: 248 DKIYINYVISNKDWSQAVNEFSWDNKFVGAQALLVS----EFYNGANDLAKFKSDVESFV 307

Query: 329 CSNLPNSPYHQIYVSPGGMIHMRDGANTQYV-TGTAFLFSAYSDILATFKQTVKCADQQF 388
           C+ +P S   QI  +PGG++ +RD +N QYV T T  LF     +      +++C   +F
Sbjct: 308 CAMMPGSSSQQIKPTPGGLLFIRDSSNLQYVTTATTVLFHYSKTLTKAGVGSIQCGSTKF 367

Query: 389 DSTQLMSFAKKQMDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPNAE-VSCPMS 448
             +Q+ +FAK Q+DY+LG NP+  SYMVGFG   P Q HHRG+S+P +    E + C   
Sbjct: 368 TVSQIRNFAKSQVDYILGNNPMKMSYMVGFGTKYPTQPHHRGSSLPSIQSKPEKIDCNGG 427

Query: 449 FVEWFNKNTPNPNELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKL 502
           +  ++N +TPNPN   GAI+GGP+ +D + DK+ D    EP TY N+  +G +A L
Sbjct: 428 Y-SYYNSDTPNPNVHIGAIVGGPNSSDQYSDKKSDYSHAEPTTYINAAFIGPVAAL 474

[Close]

BLAST of MS001389 vs. TAIR 10
Match: AT1G02800.1 (cellulase 2 )

HSP 1 Score: 440.3 bits (1131), Expect = 2.1e-123
Identity = 226/465 (48.60%), Postives = 296/465 (63.66%), Query Frame = 0


Query: 48  NYKDALSKALIFLEAQRSGKLPPNHRPAWRGDSALDDGKLANVDLEGGYYDAGDNVKYGL 107
           NYKDALSK+++F E QRSGKLPPN R  WR +S L DG   NVDL GGYYDAGDN+K+G 
Sbjct: 43  NYKDALSKSILFFEGQRSGKLPPNQRMTWRSNSGLSDGSALNVDLVGGYYDAGDNMKFGF 102

Query: 108 PMAFTVTTLSWAAMTYQAEIEAAGEMENVRAAIKWGTDYFLKACSRRNRLYVQVGDPVKD 167
           PMAFT T LSW+ + +   +++  E+ N + AI+W TD+ LKA S  + +YVQVGDP  D
Sbjct: 103 PMAFTTTMLSWSLIEFGGLMKS--ELPNAKDAIRWATDFLLKATSHPDTIYVQVGDPNMD 162

Query: 168 HECWVRPENMKTPRTVLQIDNNTPGTEIAAETSAAMASSSIVFRSVNKTYARRLLNKAKL 227
           H CW RPE+M TPR+V ++D N PG++IA E +AA+A++SIVFR  + +Y+  LL +A  
Sbjct: 163 HACWERPEDMDTPRSVFKVDKNNPGSDIAGEIAAALAAASIVFRKCDPSYSNHLLQRAIT 222

Query: 228 LFQFAKAHKGTYDG-----ECPFYCSFSGYNDELLWAATWLYIATKRPVYLKYVKEEAIT 287
           +F FA  ++G Y        CPFYCS+SGY DELLW A WL  AT  P YL Y+K     
Sbjct: 223 VFTFADKYRGPYSAGLAPEVCPFYCSYSGYQDELLWGAAWLQKATNNPTYLNYIKANGQI 282

Query: 288 GSVAE----FSWDLKYAGAQILLSKVNFIKLQQHQKGLQTLKDQADSYICSNLPNSPYHQ 347
               E    FSWD K+ GA+ILLSK   I   Q  K L+  K+ ADS+ICS LP +   Q
Sbjct: 283 LGADEFDNMFSWDNKHVGARILLSKEFLI---QKVKSLEEYKEHADSFICSVLPGASSSQ 342

Query: 348 IYVSPGGMIHMRDGANTQYVTGTAFLFSAYSDILATFKQTVKCADQQFDSTQLMSFAKKQ 407
              +PGG++     +N QYVT T+FL   Y+  L + +    C        +L S AKKQ
Sbjct: 343 --YTPGGLLFKMGESNMQYVTSTSFLLLTYAKYLTSARTVAYCGGSVVTPARLRSIAKKQ 402

Query: 408 MDYVLGINPLGRSYMVGFGNNPPVQAHHRGASVPVLAPN-AEVSCPMSFVEWFNKNTPNP 467
           +DY+LG NPL  SYMVG+G   P + HHRG+S+P +A +   + C   F   F   +PNP
Sbjct: 403 VDYLLGGNPLKMSYMVGYGLKYPRRIHHRGSSLPSVAVHPTRIQCHDGF-SLFTSQSPNP 462

Query: 468 NELTGAILGGPDKNDNFVDKRWDSPMTEPVTYTNSLAVGVIAKLA 503
           N+L GA++GGPD+ND F D+R D   +EP TY N+  VG +A LA
Sbjct: 463 NDLVGAVVGGPDQNDQFPDERSDYGRSEPATYINAPLVGALAYLA 499

The following BLAST results are available for this feature:

BLAST of MS001389 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022131779.1	4.5e-277	97.74	endoglucanase 16 [Momordica charantia]	[more]
XP_038886295.1	1.9e-243	84.32	endoglucanase 16 [Benincasa hispida]	[more]
XP_023002656.1	2.8e-234	81.39	endoglucanase 16-like [Cucurbita maxima]	[more]
XP_023537900.1	2.6e-232	80.37	endoglucanase 16-like [Cucurbita pepo subsp. pepo]	[more]
XP_022951947.1	4.4e-232	80.57	endoglucanase 16-like [Cucurbita moschata]	[more]

BLAST of MS001389 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYG3	2.0e-203	70.53	Endoglucanase 16 OS=Arabidopsis thaliana OX=3702 GN=At3g43860 PE=2 SV=1	[more]
Q6K7G9	1.3e-186	66.67	Endoglucanase 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0778600 PE=2 SV...	[more]
P22503	1.7e-125	48.14	Endoglucanase OS=Phaseolus vulgaris OX=3885 PE=2 SV=2	[more]
Q9SZ90	5.5e-124	47.48	Endoglucanase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g09740 PE=3 SV=2	[more]
O81416	9.3e-124	48.17	Endoglucanase 17 OS=Arabidopsis thaliana OX=3702 GN=At4g02290 PE=2 SV=1	[more]

BLAST of MS001389 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BR89	2.2e-277	97.74	Endoglucanase OS=Momordica charantia OX=3673 GN=LOC111004858 PE=3 SV=1	[more]
A0A6J1KLX1	1.3e-234	81.39	Endoglucanase OS=Cucurbita maxima OX=3661 GN=LOC111496447 PE=3 SV=1	[more]
A0A6J1GJ20	2.2e-232	80.57	Endoglucanase OS=Cucurbita moschata OX=3662 GN=LOC111454680 PE=3 SV=1	[more]
A0A1S3BDP2	5.3e-231	81.40	Endoglucanase OS=Cucumis melo OX=3656 GN=LOC103488526 PE=3 SV=1	[more]
A0A6J1KB06	1.0e-229	81.55	Endoglucanase OS=Cucurbita maxima OX=3661 GN=LOC111492677 PE=3 SV=1	[more]

BLAST of MS001389 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G43860.1	1.4e-204	70.53	glycosyl hydrolase 9A4	[more]
AT4G09740.1	3.9e-125	47.48	glycosyl hydrolase 9B14	[more]
AT4G02290.1	6.6e-125	48.17	glycosyl hydrolase 9B13	[more]
AT4G23560.1	8.6e-125	46.85	glycosyl hydrolase 9B15	[more]
AT1G02800.1	2.1e-123	48.60	cellulase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001701	Glycoside hydrolase family 9	PFAM	PF00759	Glyco_hydro_9	coord: 49..497 e-value: 2.1E-138 score: 462.5
IPR012341	Six-hairpin glycosidase-like superfamily	GENE3D	1.50.10.10		coord: 46..502 e-value: 9.9E-155 score: 517.8
None	No IPR available	PANTHER	PTHR22298	ENDO-1,4-BETA-GLUCANASE	coord: 26..502
None	No IPR available	PANTHER	PTHR22298:SF104	ENDOGLUCANASE 16-RELATED	coord: 26..502
IPR018221	Glycoside hydrolase family 9, His active site	PROSITE	PS00592	GH9_2	coord: 401..427
IPR008928	Six-hairpin glycosidase superfamily	SUPERFAMILY	48208	Six-hairpin glycosidases	coord: 48..501

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS001389.1	MS001389.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds