MS001357 (gene) Bitter gourd (TR) v1

Overview


Name	MS001357
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	WRKY transcription factor WRKY76-like
Location	scaffold36: 3517587 .. 3517700 (+)
RNA-Seq Expression	MS001357
Synteny	MS001357

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCGAAAGATGGATATAGATGGAGGAAATACGGACAAAAGATAACAAAGGATAATCAATATCCTCGTGCTTATTTCAAGTGTTCTTCTCCAGGATGCCCTGTCAAAAAGAAGGTA

mRNA sequence

GCGAAAGATGGATATAGATGGAGGAAATACGGACAAAAGATAACAAAGGATAATCAATATCCTCGTGCTTATTTCAAGTGTTCTTCTCCAGGATGCCCTGTCAAAAAGAAGGTA

Coding sequence (CDS)

GCGAAAGATGGATATAGATGGAGGAAATACGGACAAAAGATAACAAAGGATAATCAATATCCTCGTGCTTATTTCAAGTGTTCTTCTCCAGGATGCCCTGTCAAAAAGAAGGTA

Protein sequence

AKDGYRWRKYGQKITKDNQYPRAYFKCSSPGCPVKKKV

Homology

[Close]

BLAST of MS001357 vs. NCBI nr
Match: XP_038886179.1 (probable WRKY transcription factor 40 [Benincasa hispida])

HSP 1 Score: 82.4 bits (202), Expect = 9.1e-13
Identity = 36/37 (97.30%), Postives = 36/37 (97.30%), Query Frame = 0


Query: 2   KDGYRWRKYGQKITKDNQYPRAYFKCSSPGCPVKKKV 39
           KDGYRWRKYGQKITKDNQ PRAYFKCSSPGCPVKKKV
Sbjct: 91  KDGYRWRKYGQKITKDNQSPRAYFKCSSPGCPVKKKV 127

[Close]

BLAST of MS001357 vs. NCBI nr
Match: XP_022132094.1 (WRKY transcription factor WRKY76-like [Momordica charantia])

HSP 1 Score: 82.4 bits (202), Expect = 9.1e-13
Identity = 36/38 (94.74%), Postives = 36/38 (94.74%), Query Frame = 0


Query: 1   AKDGYRWRKYGQKITKDNQYPRAYFKCSSPGCPVKKKV 39
           AKDGYRWRKYGQKITKDNQYPRAYF CSS GCPVKKKV
Sbjct: 100 AKDGYRWRKYGQKITKDNQYPRAYFNCSSRGCPVKKKV 137

[Close]

BLAST of MS001357 vs. NCBI nr
Match: KAA0064889.1 (putative WRKY transcription factor 40 [Cucumis melo var. makuwa])

HSP 1 Score: 81.3 bits (199), Expect = 2.0e-12
Identity = 35/37 (94.59%), Postives = 36/37 (97.30%), Query Frame = 0


Query: 2   KDGYRWRKYGQKITKDNQYPRAYFKCSSPGCPVKKKV 39
           KDGY+WRKYGQKITKDNQ PRAYFKCSSPGCPVKKKV
Sbjct: 64  KDGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKKKV 100

[Close]

BLAST of MS001357 vs. NCBI nr
Match: XP_004138843.1 (probable WRKY transcription factor 60 [Cucumis sativus] >KGN62993.1 hypothetical protein Csa_022585 [Cucumis sativus])

HSP 1 Score: 81.3 bits (199), Expect = 2.0e-12
Identity = 35/37 (94.59%), Postives = 36/37 (97.30%), Query Frame = 0


Query: 2   KDGYRWRKYGQKITKDNQYPRAYFKCSSPGCPVKKKV 39
           KDGY+WRKYGQKITKDNQ PRAYFKCSSPGCPVKKKV
Sbjct: 94  KDGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKKKV 130

[Close]

BLAST of MS001357 vs. NCBI nr
Match: XP_016899977.1 (PREDICTED: probable WRKY transcription factor 40 [Cucumis melo])

HSP 1 Score: 79.7 bits (195), Expect = 5.9e-12
Identity = 34/37 (91.89%), Postives = 36/37 (97.30%), Query Frame = 0


Query: 2   KDGYRWRKYGQKITKDNQYPRAYFKCSSPGCPVKKKV 39
           KDGY+WRKYGQKITKDNQ PRAYFKCSSPGCPVKK+V
Sbjct: 64  KDGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKKEV 100

[Close]

BLAST of MS001357 vs. ExPASy Swiss-Prot
Match: B8BF91 (WRKY transcription factor WRKY62 OS=Oryza sativa subsp. indica OX=39946 GN=WRKY62 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.4e-11
Identity = 28/38 (73.68%), Postives = 35/38 (92.11%), Query Frame = 0


Query: 2   KDGYRWRKYGQKITKDNQYPRAYFKCS-SPGCPVKKKV 39
           KDGY+WRKYGQK+T+DN YPRAYF+C+ +P CPVKKK+
Sbjct: 144 KDGYQWRKYGQKVTRDNPYPRAYFRCAFAPSCPVKKKL 181

[Close]

BLAST of MS001357 vs. ExPASy Swiss-Prot
Match: Q6EPZ0 (WRKY transcription factor WRKY62 OS=Oryza sativa subsp. japonica OX=39947 GN=WRKY62 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.4e-11
Identity = 28/38 (73.68%), Postives = 35/38 (92.11%), Query Frame = 0


Query: 2   KDGYRWRKYGQKITKDNQYPRAYFKCS-SPGCPVKKKV 39
           KDGY+WRKYGQK+T+DN YPRAYF+C+ +P CPVKKK+
Sbjct: 144 KDGYQWRKYGQKVTRDNPYPRAYFRCAFAPSCPVKKKL 181

[Close]

BLAST of MS001357 vs. ExPASy Swiss-Prot
Match: Q9SI37 (WRKY transcription factor 1 OS=Arabidopsis thaliana OX=3702 GN=WRKY1 PE=1 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 4.0e-11
Identity = 27/36 (75.00%), Postives = 31/36 (86.11%), Query Frame = 0


Query: 3   DGYRWRKYGQKITKDNQYPRAYFKCSSPGCPVKKKV 39
           DGYRWRKYGQK  K + YPR+Y++CSSPGCPVKK V
Sbjct: 308 DGYRWRKYGQKSVKGSPYPRSYYRCSSPGCPVKKHV 343

[Close]

BLAST of MS001357 vs. ExPASy Swiss-Prot
Match: Q9XI90 (Probable WRKY transcription factor 4 OS=Arabidopsis thaliana OX=3702 GN=WRKY4 PE=1 SV=2)

HSP 1 Score: 67.0 bits (162), Expect = 5.2e-11
Identity = 26/38 (68.42%), Postives = 33/38 (86.84%), Query Frame = 0


Query: 1   AKDGYRWRKYGQKITKDNQYPRAYFKCSSPGCPVKKKV 39
           A DGY WRKYGQK  K +++PR+Y+KC++PGCPVKKKV
Sbjct: 228 ADDGYNWRKYGQKQVKGSEFPRSYYKCTNPGCPVKKKV 265

[Close]

BLAST of MS001357 vs. ExPASy Swiss-Prot
Match: Q9FG77 (Probable WRKY transcription factor 2 OS=Arabidopsis thaliana OX=3702 GN=WRKY2 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.2e-10
Identity = 25/38 (65.79%), Postives = 33/38 (86.84%), Query Frame = 0


Query: 1   AKDGYRWRKYGQKITKDNQYPRAYFKCSSPGCPVKKKV 39
           A+DGY WRKYGQK+ K ++YPR+Y+KC++P C VKKKV
Sbjct: 272 AEDGYNWRKYGQKLVKGSEYPRSYYKCTNPNCQVKKKV 309

[Close]

BLAST of MS001357 vs. ExPASy TrEMBL
Match: A0A6J1BS37 (WRKY transcription factor WRKY76-like OS=Momordica charantia OX=3673 GN=LOC111005051 PE=4 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 4.4e-13
Identity = 36/38 (94.74%), Postives = 36/38 (94.74%), Query Frame = 0


Query: 1   AKDGYRWRKYGQKITKDNQYPRAYFKCSSPGCPVKKKV 39
           AKDGYRWRKYGQKITKDNQYPRAYF CSS GCPVKKKV
Sbjct: 100 AKDGYRWRKYGQKITKDNQYPRAYFNCSSRGCPVKKKV 137

[Close]

BLAST of MS001357 vs. ExPASy TrEMBL
Match: A0A5A7VHE4 (Putative WRKY transcription factor 40 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002030 PE=4 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 9.9e-13
Identity = 35/37 (94.59%), Postives = 36/37 (97.30%), Query Frame = 0


Query: 2   KDGYRWRKYGQKITKDNQYPRAYFKCSSPGCPVKKKV 39
           KDGY+WRKYGQKITKDNQ PRAYFKCSSPGCPVKKKV
Sbjct: 64  KDGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKKKV 100

[Close]

BLAST of MS001357 vs. ExPASy TrEMBL
Match: A0A0A0LMJ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G382660 PE=4 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 9.9e-13
Identity = 35/37 (94.59%), Postives = 36/37 (97.30%), Query Frame = 0


Query: 2   KDGYRWRKYGQKITKDNQYPRAYFKCSSPGCPVKKKV 39
           KDGY+WRKYGQKITKDNQ PRAYFKCSSPGCPVKKKV
Sbjct: 94  KDGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKKKV 130

[Close]

BLAST of MS001357 vs. ExPASy TrEMBL
Match: A0A1S4DVG5 (probable WRKY transcription factor 40 OS=Cucumis melo OX=3656 GN=LOC103488348 PE=4 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 2.9e-12
Identity = 34/37 (91.89%), Postives = 36/37 (97.30%), Query Frame = 0


Query: 2   KDGYRWRKYGQKITKDNQYPRAYFKCSSPGCPVKKKV 39
           KDGY+WRKYGQKITKDNQ PRAYFKCSSPGCPVKK+V
Sbjct: 64  KDGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKKEV 100

[Close]

BLAST of MS001357 vs. ExPASy TrEMBL
Match: A0A5J5RGE3 (WRKY domain-containing protein OS=Gossypium barbadense OX=3634 GN=ES319_D05G060500v1 PE=4 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 4.6e-10
Identity = 29/37 (78.38%), Postives = 34/37 (91.89%), Query Frame = 0


Query: 2   KDGYRWRKYGQKITKDNQYPRAYFKCSSPGCPVKKKV 39
           +DGY+WRKYGQK+T+DN  PRAYFKCS+P CPVKKKV
Sbjct: 155 RDGYQWRKYGQKVTRDNPSPRAYFKCSAPSCPVKKKV 191

[Close]

BLAST of MS001357 vs. TAIR 10
Match: AT2G04880.1 (zinc-dependent activator protein-1 )

HSP 1 Score: 67.4 bits (163), Expect = 2.8e-12
Identity = 27/36 (75.00%), Postives = 31/36 (86.11%), Query Frame = 0


Query: 3   DGYRWRKYGQKITKDNQYPRAYFKCSSPGCPVKKKV 39
           DGYRWRKYGQK  K + YPR+Y++CSSPGCPVKK V
Sbjct: 308 DGYRWRKYGQKSVKGSPYPRSYYRCSSPGCPVKKHV 343

[Close]

BLAST of MS001357 vs. TAIR 10
Match: AT2G04880.2 (zinc-dependent activator protein-1 )

HSP 1 Score: 67.4 bits (163), Expect = 2.8e-12
Identity = 27/36 (75.00%), Postives = 31/36 (86.11%), Query Frame = 0


Query: 3   DGYRWRKYGQKITKDNQYPRAYFKCSSPGCPVKKKV 39
           DGYRWRKYGQK  K + YPR+Y++CSSPGCPVKK V
Sbjct: 284 DGYRWRKYGQKSVKGSPYPRSYYRCSSPGCPVKKHV 319

[Close]

BLAST of MS001357 vs. TAIR 10
Match: AT1G13960.1 (WRKY DNA-binding protein 4 )

HSP 1 Score: 67.0 bits (162), Expect = 3.7e-12
Identity = 26/38 (68.42%), Postives = 33/38 (86.84%), Query Frame = 0


Query: 1   AKDGYRWRKYGQKITKDNQYPRAYFKCSSPGCPVKKKV 39
           A DGY WRKYGQK  K +++PR+Y+KC++PGCPVKKKV
Sbjct: 228 ADDGYNWRKYGQKQVKGSEFPRSYYKCTNPGCPVKKKV 265

[Close]

BLAST of MS001357 vs. TAIR 10
Match: AT1G13960.2 (WRKY DNA-binding protein 4 )

HSP 1 Score: 67.0 bits (162), Expect = 3.7e-12
Identity = 26/38 (68.42%), Postives = 33/38 (86.84%), Query Frame = 0


Query: 1   AKDGYRWRKYGQKITKDNQYPRAYFKCSSPGCPVKKKV 39
           A DGY WRKYGQK  K +++PR+Y+KC++PGCPVKKKV
Sbjct: 201 ADDGYNWRKYGQKQVKGSEFPRSYYKCTNPGCPVKKKV 238

[Close]

BLAST of MS001357 vs. TAIR 10
Match: AT5G56270.1 (WRKY DNA-binding protein 2 )

HSP 1 Score: 65.9 bits (159), Expect = 8.2e-12
Identity = 25/38 (65.79%), Postives = 33/38 (86.84%), Query Frame = 0


Query: 1   AKDGYRWRKYGQKITKDNQYPRAYFKCSSPGCPVKKKV 39
           A+DGY WRKYGQK+ K ++YPR+Y+KC++P C VKKKV
Sbjct: 272 AEDGYNWRKYGQKLVKGSEYPRSYYKCTNPNCQVKKKV 309

The following BLAST results are available for this feature:

BLAST of MS001357 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038886179.1	9.1e-13	97.30	probable WRKY transcription factor 40 [Benincasa hispida]	[more]
XP_022132094.1	9.1e-13	94.74	WRKY transcription factor WRKY76-like [Momordica charantia]	[more]
KAA0064889.1	2.0e-12	94.59	putative WRKY transcription factor 40 [Cucumis melo var. makuwa]	[more]
XP_004138843.1	2.0e-12	94.59	probable WRKY transcription factor 60 [Cucumis sativus] >KGN62993.1 hypothetical...	[more]
XP_016899977.1	5.9e-12	91.89	PREDICTED: probable WRKY transcription factor 40 [Cucumis melo]	[more]

BLAST of MS001357 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B8BF91	1.4e-11	73.68	WRKY transcription factor WRKY62 OS=Oryza sativa subsp. indica OX=39946 GN=WRKY6...	[more]
Q6EPZ0	1.4e-11	73.68	WRKY transcription factor WRKY62 OS=Oryza sativa subsp. japonica OX=39947 GN=WRK...	[more]
Q9SI37	4.0e-11	75.00	WRKY transcription factor 1 OS=Arabidopsis thaliana OX=3702 GN=WRKY1 PE=1 SV=1	[more]
Q9XI90	5.2e-11	68.42	Probable WRKY transcription factor 4 OS=Arabidopsis thaliana OX=3702 GN=WRKY4 PE...	[more]
Q9FG77	1.2e-10	65.79	Probable WRKY transcription factor 2 OS=Arabidopsis thaliana OX=3702 GN=WRKY2 PE...	[more]

BLAST of MS001357 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BS37	4.4e-13	94.74	WRKY transcription factor WRKY76-like OS=Momordica charantia OX=3673 GN=LOC11100...	[more]
A0A5A7VHE4	9.9e-13	94.59	Putative WRKY transcription factor 40 OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A0A0LMJ1	9.9e-13	94.59	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G382660 PE=4 SV=1	[more]
A0A1S4DVG5	2.9e-12	91.89	probable WRKY transcription factor 40 OS=Cucumis melo OX=3656 GN=LOC103488348 PE...	[more]
A0A5J5RGE3	4.6e-10	78.38	WRKY domain-containing protein OS=Gossypium barbadense OX=3634 GN=ES319_D05G0605...	[more]

BLAST of MS001357 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G04880.1	2.8e-12	75.00	zinc-dependent activator protein-1	[more]
AT2G04880.2	2.8e-12	75.00	zinc-dependent activator protein-1	[more]
AT1G13960.1	3.7e-12	68.42	WRKY DNA-binding protein 4	[more]
AT1G13960.2	3.7e-12	68.42	WRKY DNA-binding protein 4	[more]
AT5G56270.1	8.2e-12	65.79	WRKY DNA-binding protein 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003657	WRKY domain	SMART	SM00774	WRKY_cls	coord: 1..38 e-value: 6.9E-8 score: 39.6
IPR003657	WRKY domain	PFAM	PF03106	WRKY	coord: 2..38 e-value: 3.5E-16 score: 59.2
IPR003657	WRKY domain	PROSITE	PS50811	WRKY	coord: 1..38 score: 19.221264
IPR036576	WRKY domain superfamily	GENE3D	2.20.25.80	WRKY domain	coord: 1..38 e-value: 3.0E-18 score: 67.3
IPR036576	WRKY domain superfamily	SUPERFAMILY	118290	WRKY DNA-binding domain	coord: 2..38
IPR044810	WRKY transcription factor, plant	PANTHER	PTHR31429	WRKY TRANSCRIPTION FACTOR 36-RELATED	coord: 2..38
None	No IPR available	PANTHER	PTHR31429:SF3	WRKY TRANSCRIPTION FACTOR 40-RELATED	coord: 2..38

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS001357.1	MS001357.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0043565	sequence-specific DNA binding