MS001356 (gene) Bitter gourd (TR) v1

Overview
NameMS001356
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionWRKY protein
Locationscaffold36: 3508495 .. 3510519 (+)
RNA-Seq ExpressionMS001356
SyntenyMS001356
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCTCTGCCTCCGCCACCATCAACACGTCTATCGACCTCAATTTCAATCCTCCGCCGCCCACCGCCGACGATTCTCCGGCGAATCACACTATTTACAGTTCACTCAAAGAAGATCAGGTCGTTGCACGAATAATCGGAACGAACCTTTTTTTCTTCTGAAGAACAGGGAGATTAATTACAATTAGTTGTTTGTTGTTGATCTGTGCAGGCGGCGGGTGTTCTTGTGGAGAAATTGAATCGGATAAGTTCGGAGAATGCGAAGCTGAATCAGATTATAGGGCTTGTGGTGGAGAATTACAGTGTTCTGCGAAATCAGGTGTCGGATTTGATGATAAAATCGAGATCGAAGCGAAAGGCCGGATGCGATAACTGCAATTCGAATCGGAGCGGTAATAATAGTAATGTTTTTACGGATCAGTACTGCGGATGTTGCAGCGACGACGATTCGTGTTATAAAAGGCCTAGAGAAAACAGTAAGCCCAAGGTTAAACGAGTTCTGGTTCCCACGCCGCTATCTGATTCAACTCTGGTATGTTCATGTTTATGTTACCACATTTCAATTTTTTTTTTCATTCAATCTTCAACTTCTTTCTAATTCCGGTGGATTGACTCAAGAAATATGTATTCCTATTCATTTACTTTTATTGTGCTAAGAAATAAATATGCCATGCTTGGGTAAAGATATCTGTACTTTTCTTTGAGACGAAAGGATCAAATCTCCATATCTCACTGTTTGTTTTCCCAATAAATAAATGGTTCCCAAGAGTAGCTTAGTATACGAGAATTTAGACTATCTTGGTCATATTTTTTTTGGTTTNTGATAAGGAAAAGAAAAGGTAGAATTGAACTAGTTCATTTATATTAGTTTGTTAAATTAATTAGAATAGTTTTGATGGGATTAAATCTTGCGATATTAATGGAACTCTCTAGTATTCATATATTAACCACATATTTGTGGCTGATTAATCAAAAATTGACGTGACAATTTATGCTAATTTTAATATTTAGAATTCTTCATCTTGTTCTTCTTTCGTTTTGTTTTTTTAGATGATAATTCCAATTGGATATGGTGTAAAACTATAGGTGGTGAAGGATGGATATCAATGGAGGAAATATGGTCAAAAGGTGACTAAAGACAACCCCTCCCCTAGAGCTTACTATAAATGCTCCTTTGCCCCCAGCTGCCCCGTCAAAAAGAAGGTAATTTCCTCGAACCTTCTCCCTCTCAATCTTTCTTTCTCACCCTATTTAATTTTCAACCCCATTCTTCTATCCTAATTCCTATTCATTCAAATAATATTAGACTTCTTGCATAAAATTCACATCATTGCATAAGAAAAAAAAGTGTAATCCTCGTTATTTCTTCGCCATCTATTCCTAATTTCCAATTGTATTAATAATGGCCAACATGAACATTGGTAACATTCTTGTTTCTTGTTTCTTGTTTCTATTTCTACATTTTTTAATAATGTAACATAGAATTGCACGTTCTCAACATCCATAAATTAAATAATGATAAAGATAATATTGTATCTTATATTTTGGAACAAATTGAAAATGAAACAAAATTTTTTTGTTTTTAAAATTTCTATACCATTTTGAGAGAATGCTTCGGAGAAATCATATATATATTTGTCGAATGCTTAGAAAAATTACCAAATGGATCCTTCCCTTTTTTATCATTGAACTTATATTTCATTTGGACAAATATTACAGGTGCAAAGGAGTGCTCAAGACCCATCATTTTTAGTGGCTACATACGAAGGAGAGCACAATCACATGCAACCCAATTCTGGAATTGAGTATCAATTGGTTGGACCAATTCAATTGGGTTCAAATAAGCTGGAATCTTCTGTTTCATCTCCACCGTCCATGAGATCCCCCTCATCATCTTCTGTTGACACTCTCAAAGCTTCACTTCCATCAACCCCACAAATTCTAGTTCAACAAATGGCTTCCTTCCTGACCAGAGACCCCAATTTCACTACAGCCCTTGCCACTGCCATTACTGGAACCATGGTAGAT

mRNA sequence

CCCTCTGCCTCCGCCACCATCAACACGTCTATCGACCTCAATTTCAATCCTCCGCCGCCCACCGCCGACGATTCTCCGGCGAATCACACTATTTACAGTTCACTCAAAGAAGATCAGGCGGCGGGTGTTCTTGTGGAGAAATTGAATCGGATAAGTTCGGAGAATGCGAAGCTGAATCAGATTATAGGGCTTGTGGTGGAGAATTACAGTGTTCTGCGAAATCAGGTGTCGGATTTGATGATAAAATCGAGATCGAAGCGAAAGGCCGGATGCGATAACTGCAATTCGAATCGGAGCGGTAATAATAGTAATGTTTTTACGGATCAGTACTGCGGATGTTGCAGCGACGACGATTCGTGTTATAAAAGGCCTAGAGAAAACAGTAAGCCCAAGGTTAAACGAGTTCTGGTTCCCACGCCGCTATCTGATTCAACTCTGGTGGTGAAGGATGGATATCAATGGAGGAAATATGGTCAAAAGGTGACTAAAGACAACCCCTCCCCTAGAGCTTACTATAAATGCTCCTTTGCCCCCAGCTGCCCCGTCAAAAAGAAGGTGCAAAGGAGTGCTCAAGACCCATCATTTTTAGTGGCTACATACGAAGGAGAGCACAATCACATGCAACCCAATTCTGGAATTGAGTATCAATTGGTTGGACCAATTCAATTGGGTTCAAATAAGCTGGAATCTTCTGTTTCATCTCCACCGTCCATGAGATCCCCCTCATCATCTTCTGTTGACACTCTCAAAGCTTCACTTCCATCAACCCCACAAATTCTAGTTCAACAAATGGCTTCCTTCCTGACCAGAGACCCCAATTTCACTACAGCCCTTGCCACTGCCATTACTGGAACCATGGTAGAT

Coding sequence (CDS)

CCCTCTGCCTCCGCCACCATCAACACGTCTATCGACCTCAATTTCAATCCTCCGCCGCCCACCGCCGACGATTCTCCGGCGAATCACACTATTTACAGTTCACTCAAAGAAGATCAGGCGGCGGGTGTTCTTGTGGAGAAATTGAATCGGATAAGTTCGGAGAATGCGAAGCTGAATCAGATTATAGGGCTTGTGGTGGAGAATTACAGTGTTCTGCGAAATCAGGTGTCGGATTTGATGATAAAATCGAGATCGAAGCGAAAGGCCGGATGCGATAACTGCAATTCGAATCGGAGCGGTAATAATAGTAATGTTTTTACGGATCAGTACTGCGGATGTTGCAGCGACGACGATTCGTGTTATAAAAGGCCTAGAGAAAACAGTAAGCCCAAGGTTAAACGAGTTCTGGTTCCCACGCCGCTATCTGATTCAACTCTGGTGGTGAAGGATGGATATCAATGGAGGAAATATGGTCAAAAGGTGACTAAAGACAACCCCTCCCCTAGAGCTTACTATAAATGCTCCTTTGCCCCCAGCTGCCCCGTCAAAAAGAAGGTGCAAAGGAGTGCTCAAGACCCATCATTTTTAGTGGCTACATACGAAGGAGAGCACAATCACATGCAACCCAATTCTGGAATTGAGTATCAATTGGTTGGACCAATTCAATTGGGTTCAAATAAGCTGGAATCTTCTGTTTCATCTCCACCGTCCATGAGATCCCCCTCATCATCTTCTGTTGACACTCTCAAAGCTTCACTTCCATCAACCCCACAAATTCTAGTTCAACAAATGGCTTCCTTCCTGACCAGAGACCCCAATTTCACTACAGCCCTTGCCACTGCCATTACTGGAACCATGGTAGAT

Protein sequence

PSASATINTSIDLNFNPPPPTADDSPANHTIYSSLKEDQAAGVLVEKLNRISSENAKLNQIIGLVVENYSVLRNQVSDLMIKSRSKRKAGCDNCNSNRSGNNSNVFTDQYCGCCSDDDSCYKRPRENSKPKVKRVLVPTPLSDSTLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCPVKKKVQRSAQDPSFLVATYEGEHNHMQPNSGIEYQLVGPIQLGSNKLESSVSSPPSMRSPSSSSVDTLKASLPSTPQILVQQMASFLTRDPNFTTALATAITGTMVD
Homology
BLAST of MS001356 vs. NCBI nr
Match: XP_022131459.1 (probable WRKY transcription factor 40 [Momordica charantia])

HSP 1 Score: 561.6 bits (1446), Expect = 3.9e-156
Identity = 286/288 (99.31%), Postives = 287/288 (99.65%), Query Frame = 0

Query: 1   PSASATINTSIDLNFNPPPPTADDSPANHTIYSSLKEDQAAGVLVEKLNRISSENAKLNQ 60
           PSASATINTS+DLNFNPPPPTADDSPANHTIYSSLKEDQAAGVLVEKLNRISSENAKLNQ
Sbjct: 3   PSASATINTSLDLNFNPPPPTADDSPANHTIYSSLKEDQAAGVLVEKLNRISSENAKLNQ 62

Query: 61  IIGLVVENYSVLRNQVSDLMIKSRSKRKAGCDNCNSNRSGNNSNVFTDQYCGCCSDDDSC 120
           IIGLVVENYSVLRNQVSDLMIKSRSKRKAGCDNCNSNRSGNNSNVFTDQYCGCCSDDDSC
Sbjct: 63  IIGLVVENYSVLRNQVSDLMIKSRSKRKAGCDNCNSNRSGNNSNVFTDQYCGCCSDDDSC 122

Query: 121 YKRPRENSKPKVKRVLVPTPLSDSTLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSC 180
           YKRPRENSKPKVKRVLVPTPLSDSTLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSC
Sbjct: 123 YKRPRENSKPKVKRVLVPTPLSDSTLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSC 182

Query: 181 PVKKKVQRSAQDPSFLVATYEGEHNHMQPNSGIEYQLVGPIQLGSNKLESSVSSPPSMRS 240
           PVKKKVQRSAQDPSFLVATYEGEHNHMQPNSGIEYQLVGPIQLGSNKLESSV SPPSMRS
Sbjct: 183 PVKKKVQRSAQDPSFLVATYEGEHNHMQPNSGIEYQLVGPIQLGSNKLESSVLSPPSMRS 242

Query: 241 PSSSSVDTLKASLPSTPQILVQQMASFLTRDPNFTTALATAITGTMVD 289
           PSSSSVDTLKASLPSTPQILVQQMASFLTRDPNFTTALATAITGTMVD
Sbjct: 243 PSSSSVDTLKASLPSTPQILVQQMASFLTRDPNFTTALATAITGTMVD 290

BLAST of MS001356 vs. NCBI nr
Match: XP_023545515.1 (probable WRKY transcription factor 40 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 393.7 bits (1010), Expect = 1.4e-105
Identity = 221/310 (71.29%), Postives = 245/310 (79.03%), Query Frame = 0

Query: 4   SATINTSIDLNFNPPPPTADDSPANHTIYSSLKEDQAAGVLVEKLNRISSENAKLNQIIG 63
           SATINTS+DLNFNPPP T D+SPA  T+ + LK +  A VL EKLNR+SSEN KLNQ++G
Sbjct: 4   SATINTSLDLNFNPPPYTDDESPATSTL-TPLKAETPA-VLAEKLNRMSSENQKLNQMLG 63

Query: 64  LVVENYSVLRNQVSDLMIKSRSKRKAGCDNCNSNRSGNNSNVFTDQYCGCCSDDDSCYKR 123
            VVENYS+L+NQV  LM KSR KRKAGCD+CN NR+G      +DQYCGCCSDDDSCYKR
Sbjct: 64  RVVENYSLLQNQVIRLMFKSR-KRKAGCDSCNFNRNG------SDQYCGCCSDDDSCYKR 123

Query: 124 PRENSKPKVKRVLVPTPLSDSTLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCPVK 183
           PRE+SKPKV RVLV TP+SDS+LVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCPVK
Sbjct: 124 PRESSKPKVMRVLVSTPVSDSSLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCPVK 183

Query: 184 KKVQRSAQDPSFLVATYEGEHNHMQPNSGIEYQLVGPIQLGSNKLESSVSSPPS------ 243
           +KVQRS +DPS+LVATYEGEHNH +PNSGIEYQLVGPIQLGSN L+SSVSSP S      
Sbjct: 184 RKVQRSVEDPSYLVATYEGEHNHPKPNSGIEYQLVGPIQLGSN-LDSSVSSPSSSVVKGP 243

Query: 244 ----------MRSPSSSSVDTLKASLP---------STPQILVQQMASFLTRDPNFTTAL 289
                       S S S+  T+KAS+P         ST  ILVQQMAS LTRDPNFT AL
Sbjct: 244 SIMPSVVTYDYLSKSPSTTTTVKASVPELETPPASSSTQTILVQQMASLLTRDPNFTRAL 303

BLAST of MS001356 vs. NCBI nr
Match: KAG6598546.1 (putative WRKY transcription factor 40, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029479.1 putative WRKY transcription factor 40, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 392.1 bits (1006), Expect = 4.1e-105
Identity = 220/310 (70.97%), Postives = 244/310 (78.71%), Query Frame = 0

Query: 4   SATINTSIDLNFNPPPPTADDSPANHTIYSSLKEDQAAGVLVEKLNRISSENAKLNQIIG 63
           SATIN S+DLNFNPPP T D+SPA   + + LK +  A VL EKLNR+SSEN KLNQ++G
Sbjct: 4   SATINASLDLNFNPPPYTDDESPATSNL-TPLKSETPA-VLAEKLNRMSSENQKLNQMLG 63

Query: 64  LVVENYSVLRNQVSDLMIKSRSKRKAGCDNCNSNRSGNNSNVFTDQYCGCCSDDDSCYKR 123
            VVENYS+L+NQV  LM KSR KRKAGCD+CN NRSG      +DQYCGCCSDDDSCYKR
Sbjct: 64  RVVENYSLLQNQVIRLMFKSR-KRKAGCDSCNFNRSG------SDQYCGCCSDDDSCYKR 123

Query: 124 PRENSKPKVKRVLVPTPLSDSTLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCPVK 183
           PRE+SKPKV RVLV TP+SDS+LVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCPVK
Sbjct: 124 PRESSKPKVMRVLVSTPVSDSSLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCPVK 183

Query: 184 KKVQRSAQDPSFLVATYEGEHNHMQPNSGIEYQLVGPIQLGSNKLESSVSSPPS------ 243
           +KVQRS +DPS+LVATYEGEHNH +PNSGIEYQLVGPIQLGSN L+SSVSSP S      
Sbjct: 184 RKVQRSVEDPSYLVATYEGEHNHPKPNSGIEYQLVGPIQLGSN-LDSSVSSPSSSVVKGP 243

Query: 244 ----------MRSPSSSSVDTLKASLP---------STPQILVQQMASFLTRDPNFTTAL 289
                       S S S+ +T+KAS+P         ST  ILVQQMAS LTRDPNFT AL
Sbjct: 244 SIMPSVVTYDYLSKSPSTTNTVKASVPELETPPASSSTQTILVQQMASLLTRDPNFTRAL 303

BLAST of MS001356 vs. NCBI nr
Match: XP_022996454.1 (probable WRKY transcription factor 40 [Cucurbita maxima])

HSP 1 Score: 389.8 bits (1000), Expect = 2.0e-104
Identity = 220/310 (70.97%), Postives = 243/310 (78.39%), Query Frame = 0

Query: 4   SATINTSIDLNFNPPPPTADDSPANHTIYSSLKEDQAAGVLVEKLNRISSENAKLNQIIG 63
           SATINTS+DLNFNPPP T D+SPA  T+ + LK +  A VL E LNR+SSEN KLNQ++G
Sbjct: 4   SATINTSLDLNFNPPPYTDDESPATSTL-TPLKAETPA-VLSEMLNRMSSENQKLNQMLG 63

Query: 64  LVVENYSVLRNQVSDLMIKSRSKRKAGCDNCNSNRSGNNSNVFTDQYCGCCSDDDSCYKR 123
            VVENYS+L+NQV  LM KSR KRK GCD CN NRSG      +DQYC CCSDDDSCYKR
Sbjct: 64  RVVENYSLLQNQVIRLMFKSR-KRKTGCDGCNFNRSG------SDQYCSCCSDDDSCYKR 123

Query: 124 PRENSKPKVKRVLVPTPLSDSTLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCPVK 183
           PRE+SKPKV RVLV TP+SDS+LVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCPVK
Sbjct: 124 PRESSKPKVMRVLVLTPVSDSSLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCPVK 183

Query: 184 KKVQRSAQDPSFLVATYEGEHNHMQPNSGIEYQLVGPIQLGSNKLESSVSSPPS------ 243
           +KVQRS +DPS+LVATYEGEHNH +PNSGIEYQLVGPIQLGSN L+SSVSSP S      
Sbjct: 184 RKVQRSVEDPSYLVATYEGEHNHPKPNSGIEYQLVGPIQLGSN-LDSSVSSPSSSVVKGP 243

Query: 244 ----------MRSPSSSSVDTLKASLP---------STPQILVQQMASFLTRDPNFTTAL 289
                       S S S+ +T+KAS+P         ST  ILVQQMASFLTRDPNFT AL
Sbjct: 244 SIMPSVVTYDYLSKSPSTTNTVKASVPELETPPASSSTQTILVQQMASFLTRDPNFTRAL 303

BLAST of MS001356 vs. NCBI nr
Match: XP_022961730.1 (probable WRKY transcription factor 40 [Cucurbita moschata])

HSP 1 Score: 389.4 bits (999), Expect = 2.6e-104
Identity = 220/310 (70.97%), Postives = 244/310 (78.71%), Query Frame = 0

Query: 4   SATINTSIDLNFNPPPPTADDSPANHTIYSSLKEDQAAGVLVEKLNRISSENAKLNQIIG 63
           SATINTS+DLNFNPPP T D+SPA   + + LK +  A VL EKLNR+SSEN KLNQ++G
Sbjct: 4   SATINTSLDLNFNPPPYTDDESPATSNL-TPLKSETPA-VLAEKLNRMSSENQKLNQMLG 63

Query: 64  LVVENYSVLRNQVSDLMIKSRSKRKAGCDNCNSNRSGNNSNVFTDQYCGCCSDDDSCYKR 123
            VVENYS+L+NQV  LM KSR KRKAG D+CN NRSG      +DQYCGCCSDDDSCYKR
Sbjct: 64  RVVENYSLLQNQVIRLMFKSR-KRKAGGDSCNFNRSG------SDQYCGCCSDDDSCYKR 123

Query: 124 PRENSKPKVKRVLVPTPLSDSTLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCPVK 183
           PRE+SKPKV RVLV TP+SDS+LVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCPVK
Sbjct: 124 PRESSKPKVMRVLVSTPVSDSSLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCPVK 183

Query: 184 KKVQRSAQDPSFLVATYEGEHNHMQPNSGIEYQLVGPIQLGSNKLESSVSSPPS------ 243
           +KVQRS +DPS+LVATYEGEHNH +PNSGIEYQLVGPIQLGSN L+SSVSSP S      
Sbjct: 184 RKVQRSVEDPSYLVATYEGEHNHPKPNSGIEYQLVGPIQLGSN-LDSSVSSPSSSVVKGP 243

Query: 244 ----------MRSPSSSSVDTLKASLP---------STPQILVQQMASFLTRDPNFTTAL 289
                       S S S+ +T+KAS+P         ST  ILVQQMAS LTRDPNFT AL
Sbjct: 244 SIMPSVVTYDYLSKSPSTTNTVKASVPELETPPASSSTQTILVQQMASLLTRDPNFTRAL 303

BLAST of MS001356 vs. ExPASy Swiss-Prot
Match: Q9SAH7 (Probable WRKY transcription factor 40 OS=Arabidopsis thaliana OX=3702 GN=WRKY40 PE=1 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 4.8e-48
Identity = 125/304 (41.12%), Postives = 175/304 (57.57%), Query Frame = 0

Query: 3   ASATINTSIDLNFNPPPPTADDSPANHTIYSSLKEDQAAGVLVEKLNRISSENAKLNQII 62
           +S+ ++TS+DL                     ++ED     LVE+LNR+S+EN KL++++
Sbjct: 5   SSSLVDTSLDLTIG-------------VTRMRVEEDPPTSALVEELNRVSAENKKLSEML 64

Query: 63  GLVVENYSVLRNQVSDLMIKSRSKRKAGCDNCNSNRSGNNSNVFTDQYCGCCSD------ 122
            L+ +NY+VLR Q+ + + KS    +         +S    + F+    G  S+      
Sbjct: 65  TLMCDNYNVLRKQLMEYVNKSNITERDQISPPKKRKSPAREDAFSCAVIGGVSESSSTDQ 124

Query: 123 DDSCYKRPRENS--KPKVKRVLVPTPLSDSTLVVKDGYQWRKYGQKVTKDNPSPRAYYKC 182
           D+   K+ RE +  K KV RV   T  SD+TLVVKDGYQWRKYGQKVT+DNPSPRAY+KC
Sbjct: 125 DEYLCKKQREETVVKEKVSRVYYKTEASDTTLVVKDGYQWRKYGQKVTRDNPSPRAYFKC 184

Query: 183 SFAPSCPVKKKVQRSAQDPSFLVATYEGEHNHMQP-----NSGIEYQL----VGPIQLGS 242
           + APSC VKKKVQRS +D S LVATYEGEHNH  P     N+G+   +         + +
Sbjct: 185 ACAPSCSVKKKVQRSVEDQSVLVATYEGEHNHPMPSQIDSNNGLNRHISHGGSASTPVAA 244

Query: 243 NKLESSVSSPPS---MRSPSSSSVDTLKASLPSTPQILVQQMASFLTRDPNFTTALATAI 287
           N+  SS++ P +   M      +  T +   P   ++LV+QMAS LT+DPNFT ALA A+
Sbjct: 245 NR-RSSLTVPVTTVDMIESKKVTSPTSRIDFPQVQKLLVEQMASSLTKDPNFTAALAAAV 294

BLAST of MS001356 vs. ExPASy Swiss-Prot
Match: Q9C5T4 (WRKY transcription factor 18 OS=Arabidopsis thaliana OX=3702 GN=WRKY18 PE=1 SV=2)

HSP 1 Score: 182.2 bits (461), Expect = 8.4e-45
Identity = 127/313 (40.58%), Postives = 174/313 (55.59%), Query Frame = 0

Query: 4   SATINTSIDLNFNP-----PPPTADDSPANHTIYSSLKEDQAAGVLVEKLNRISSENAKL 63
           S+ ++ S+DLN NP     P        + H     L++D++A  L E+LNR++SEN KL
Sbjct: 4   SSFLDISLDLNTNPFSAKLPKKEVSVLASTHLKRKWLEQDESASELREELNRVNSENKKL 63

Query: 64  NQIIGLVVENYSVLRNQVSDLMIKSR----------SKRKAGCDNC-----------NSN 123
            +++  V E+Y+ L N +  L  +             KRK   D               N
Sbjct: 64  TEMLARVCESYNELHNHLEKLQSRQSPEIEQTDIPIKKRKQDPDEFLGFPIGLSSGKTEN 123

Query: 124 RSGNNSNVFTDQYCGCCSDDDSCYKRPRENS--KPKVKRVLVPTPLSDSTLVVKDGYQWR 183
            S N  +    Q     +   SC KRP  +S  K KV  V VPT  SD++L VKDG+QWR
Sbjct: 124 SSSNEDHHHHHQQHEQKNQLLSC-KRPVTDSFNKAKVSTVYVPTETSDTSLTVKDGFQWR 183

Query: 184 KYGQKVTKDNPSPRAYYKCSFAPSCPVKKKVQRSAQDPSFLVATYEGEHNHMQPNSGIEY 243
           KYGQKVT+DNPSPRAY++CSFAPSCPVKKKVQRSA+DPS LVATYEG HNH+ PN+    
Sbjct: 184 KYGQKVTRDNPSPRAYFRCSFAPSCPVKKKVQRSAEDPSLLVATYEGTHNHLGPNAS--- 243

Query: 244 QLVGPIQLGSNKLESSVSSPPSMRSPSSSSVDTLKASLPSTPQILVQQMASFLTRDPNFT 289
           +     Q GS+ +   + +     +   +  D       +  ++L+QQMAS LT+D  FT
Sbjct: 244 EGDATSQGGSSTVTLDLVNGCHRLALEKNERDN------TMQEVLIQQMASSLTKDSKFT 303

BLAST of MS001356 vs. ExPASy Swiss-Prot
Match: Q9SK33 (Probable WRKY transcription factor 60 OS=Arabidopsis thaliana OX=3702 GN=WRKY60 PE=1 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 9.6e-41
Identity = 110/283 (38.87%), Postives = 157/283 (55.48%), Query Frame = 0

Query: 12  DLNFNPPPPTADDSPANHTIYSS--LKEDQAAGVLVEKLNRISSENAKLNQIIGLVVENY 71
           DL+F+   P  + S +   +     L +D+   +L +++NR++SEN KL +++  V E Y
Sbjct: 11  DLHFSGKLPKREVSASASKVVEKKWLVKDEKRNMLQDEINRVNSENKKLTEMLARVCEKY 70

Query: 72  SVLRNQVSDLMIKSRSKRKAGCDNCNSNRSGNNSNVFTDQYCGCCSDDDSCYKRPRE--- 131
             L N    LM + +S++     N  + +         D++    S        P E   
Sbjct: 71  YALNN----LMEELQSRKSPESVNFQNKQLTGKRKQELDEF---VSSPIGLSLGPIENIT 130

Query: 132 NSKPKVKRVLVPTPLSDSTLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCPVKKKV 191
           N K  V         SD++L VKDGYQWRKYGQK+T+DNPSPRAY++CSF+PSC VKKKV
Sbjct: 131 NDKATVSTAYFAAEKSDTSLTVKDGYQWRKYGQKITRDNPSPRAYFRCSFSPSCLVKKKV 190

Query: 192 QRSAQDPSFLVATYEGEHNHMQPNSGIEYQL-VGPIQLGSNKLESSVSSPPSMRSPSSSS 251
           QRSA+DPSFLVATYEG HNH  P++ +   + +  +Q G   +E                
Sbjct: 191 QRSAEDPSFLVATYEGTHNHTGPHASVSRTVKLDLVQGGLEPVEE--------------- 250

Query: 252 VDTLKASLPSTPQILVQQMASFLTRDPNFTTALATAITGTMVD 289
               K    +  ++LVQQMAS LT+DP FT ALATAI+G +++
Sbjct: 251 ----KKERGTIQEVLVQQMASSLTKDPKFTAALATAISGRLIE 267

BLAST of MS001356 vs. ExPASy Swiss-Prot
Match: Q6IEL0 (WRKY transcription factor WRKY71 OS=Oryza sativa subsp. indica OX=39946 GN=WRKY71 PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 3.1e-39
Identity = 110/301 (36.54%), Postives = 152/301 (50.50%), Query Frame = 0

Query: 35  LKEDQAAGVLVEKLNRISSENAKLNQIIGLVVENYSVLRNQVSDLMIKSRSKRKAGCDNC 94
           LK+D     L  +L R+ +EN +L++++  V   Y  L++Q SD++  S +    G +N 
Sbjct: 44  LKKDPEVAALEAELKRMGAENRQLSEMLAAVAAKYEALQSQFSDMVTASANNGGGGGNNQ 103

Query: 95  NSNRSG------------------------------NNSNVFTDQYCGCCSDDDSC---- 154
           +S   G                              ++ +V         +D   C    
Sbjct: 104 SSTSEGGSVSPSRKRKSESLDDSPPPPPPPHPHAAPHHMHVMPGAAAAGYADQTECTSGE 163

Query: 155 -YKRPRENSKPKVKRVLVPTPLSDSTLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPS 214
             KR RE  KPK+ ++ V    SD +LVVKDGYQWRKYGQKVTKDNP PRAY++CSFAP+
Sbjct: 164 PCKRIREECKPKISKLYVHADPSDLSLVVKDGYQWRKYGQKVTKDNPCPRAYFRCSFAPA 223

Query: 215 CPVKKKVQRSAQDPSFLVATYEGEHNHMQPNSGIEYQLVGPIQLGSNKLESSVSSPPSMR 274
           CPVKKKVQRSA+D + LVATYEGEHNH QP   +  Q       GS K        P+  
Sbjct: 224 CPVKKKVQRSAEDNTILVATYEGEHNHGQPPPPL--QSAAQNSDGSGKSAGKPPHAPAAA 283

Query: 275 SPSS------------SSVDTLKASLPSTPQILVQQMASFLTRDPNFTTALATAITGTMV 289
            P+             +      A+     + L +QMA  LTRDP+F  AL TA++G ++
Sbjct: 284 PPAPVVPHRQHEPVVVNGEQQAAAASEMIRRNLAEQMAMTLTRDPSFKAALVTALSGRIL 342

BLAST of MS001356 vs. ExPASy Swiss-Prot
Match: Q6QHD1 (WRKY transcription factor WRKY71 OS=Oryza sativa subsp. japonica OX=39947 GN=WRKY71 PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 3.1e-39
Identity = 110/301 (36.54%), Postives = 152/301 (50.50%), Query Frame = 0

Query: 35  LKEDQAAGVLVEKLNRISSENAKLNQIIGLVVENYSVLRNQVSDLMIKSRSKRKAGCDNC 94
           LK+D     L  +L R+ +EN +L++++  V   Y  L++Q SD++  S +    G +N 
Sbjct: 44  LKKDPEVAALEAELKRMGAENRQLSEMLAAVAAKYEALQSQFSDMVTASANNGGGGGNNP 103

Query: 95  NSNRSG------------------------------NNSNVFTDQYCGCCSDDDSC---- 154
           +S   G                              ++ +V         +D   C    
Sbjct: 104 SSTSEGGSVSPSRKRKSESLDDSPPPPPPPHPHAAPHHMHVMPGAAAAGYADQTECTSGE 163

Query: 155 -YKRPRENSKPKVKRVLVPTPLSDSTLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPS 214
             KR RE  KPK+ ++ V    SD +LVVKDGYQWRKYGQKVTKDNP PRAY++CSFAP+
Sbjct: 164 PCKRIREECKPKISKLYVHADPSDLSLVVKDGYQWRKYGQKVTKDNPCPRAYFRCSFAPA 223

Query: 215 CPVKKKVQRSAQDPSFLVATYEGEHNHMQPNSGIEYQLVGPIQLGSNKLESSVSSPPSMR 274
           CPVKKKVQRSA+D + LVATYEGEHNH QP   +  Q       GS K        P+  
Sbjct: 224 CPVKKKVQRSAEDNTILVATYEGEHNHGQPPPPL--QSAAQNSDGSGKSAGKPPHAPAAA 283

Query: 275 SPSS------------SSVDTLKASLPSTPQILVQQMASFLTRDPNFTTALATAITGTMV 289
            P+             +      A+     + L +QMA  LTRDP+F  AL TA++G ++
Sbjct: 284 PPAPVVPHRQHEPVVVNGEQQAAAASEMIRRNLAEQMAMTLTRDPSFKAALVTALSGRIL 342

BLAST of MS001356 vs. ExPASy TrEMBL
Match: A0A6J1BQA8 (probable WRKY transcription factor 40 OS=Momordica charantia OX=3673 GN=LOC111004661 PE=4 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 1.9e-156
Identity = 286/288 (99.31%), Postives = 287/288 (99.65%), Query Frame = 0

Query: 1   PSASATINTSIDLNFNPPPPTADDSPANHTIYSSLKEDQAAGVLVEKLNRISSENAKLNQ 60
           PSASATINTS+DLNFNPPPPTADDSPANHTIYSSLKEDQAAGVLVEKLNRISSENAKLNQ
Sbjct: 3   PSASATINTSLDLNFNPPPPTADDSPANHTIYSSLKEDQAAGVLVEKLNRISSENAKLNQ 62

Query: 61  IIGLVVENYSVLRNQVSDLMIKSRSKRKAGCDNCNSNRSGNNSNVFTDQYCGCCSDDDSC 120
           IIGLVVENYSVLRNQVSDLMIKSRSKRKAGCDNCNSNRSGNNSNVFTDQYCGCCSDDDSC
Sbjct: 63  IIGLVVENYSVLRNQVSDLMIKSRSKRKAGCDNCNSNRSGNNSNVFTDQYCGCCSDDDSC 122

Query: 121 YKRPRENSKPKVKRVLVPTPLSDSTLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSC 180
           YKRPRENSKPKVKRVLVPTPLSDSTLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSC
Sbjct: 123 YKRPRENSKPKVKRVLVPTPLSDSTLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSC 182

Query: 181 PVKKKVQRSAQDPSFLVATYEGEHNHMQPNSGIEYQLVGPIQLGSNKLESSVSSPPSMRS 240
           PVKKKVQRSAQDPSFLVATYEGEHNHMQPNSGIEYQLVGPIQLGSNKLESSV SPPSMRS
Sbjct: 183 PVKKKVQRSAQDPSFLVATYEGEHNHMQPNSGIEYQLVGPIQLGSNKLESSVLSPPSMRS 242

Query: 241 PSSSSVDTLKASLPSTPQILVQQMASFLTRDPNFTTALATAITGTMVD 289
           PSSSSVDTLKASLPSTPQILVQQMASFLTRDPNFTTALATAITGTMVD
Sbjct: 243 PSSSSVDTLKASLPSTPQILVQQMASFLTRDPNFTTALATAITGTMVD 290

BLAST of MS001356 vs. ExPASy TrEMBL
Match: A0A6J1K8R9 (probable WRKY transcription factor 40 OS=Cucurbita maxima OX=3661 GN=LOC111491698 PE=4 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 9.8e-105
Identity = 220/310 (70.97%), Postives = 243/310 (78.39%), Query Frame = 0

Query: 4   SATINTSIDLNFNPPPPTADDSPANHTIYSSLKEDQAAGVLVEKLNRISSENAKLNQIIG 63
           SATINTS+DLNFNPPP T D+SPA  T+ + LK +  A VL E LNR+SSEN KLNQ++G
Sbjct: 4   SATINTSLDLNFNPPPYTDDESPATSTL-TPLKAETPA-VLSEMLNRMSSENQKLNQMLG 63

Query: 64  LVVENYSVLRNQVSDLMIKSRSKRKAGCDNCNSNRSGNNSNVFTDQYCGCCSDDDSCYKR 123
            VVENYS+L+NQV  LM KSR KRK GCD CN NRSG      +DQYC CCSDDDSCYKR
Sbjct: 64  RVVENYSLLQNQVIRLMFKSR-KRKTGCDGCNFNRSG------SDQYCSCCSDDDSCYKR 123

Query: 124 PRENSKPKVKRVLVPTPLSDSTLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCPVK 183
           PRE+SKPKV RVLV TP+SDS+LVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCPVK
Sbjct: 124 PRESSKPKVMRVLVLTPVSDSSLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCPVK 183

Query: 184 KKVQRSAQDPSFLVATYEGEHNHMQPNSGIEYQLVGPIQLGSNKLESSVSSPPS------ 243
           +KVQRS +DPS+LVATYEGEHNH +PNSGIEYQLVGPIQLGSN L+SSVSSP S      
Sbjct: 184 RKVQRSVEDPSYLVATYEGEHNHPKPNSGIEYQLVGPIQLGSN-LDSSVSSPSSSVVKGP 243

Query: 244 ----------MRSPSSSSVDTLKASLP---------STPQILVQQMASFLTRDPNFTTAL 289
                       S S S+ +T+KAS+P         ST  ILVQQMASFLTRDPNFT AL
Sbjct: 244 SIMPSVVTYDYLSKSPSTTNTVKASVPELETPPASSSTQTILVQQMASFLTRDPNFTRAL 303

BLAST of MS001356 vs. ExPASy TrEMBL
Match: A0A6J1HD49 (probable WRKY transcription factor 40 OS=Cucurbita moschata OX=3662 GN=LOC111462413 PE=4 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 1.3e-104
Identity = 220/310 (70.97%), Postives = 244/310 (78.71%), Query Frame = 0

Query: 4   SATINTSIDLNFNPPPPTADDSPANHTIYSSLKEDQAAGVLVEKLNRISSENAKLNQIIG 63
           SATINTS+DLNFNPPP T D+SPA   + + LK +  A VL EKLNR+SSEN KLNQ++G
Sbjct: 4   SATINTSLDLNFNPPPYTDDESPATSNL-TPLKSETPA-VLAEKLNRMSSENQKLNQMLG 63

Query: 64  LVVENYSVLRNQVSDLMIKSRSKRKAGCDNCNSNRSGNNSNVFTDQYCGCCSDDDSCYKR 123
            VVENYS+L+NQV  LM KSR KRKAG D+CN NRSG      +DQYCGCCSDDDSCYKR
Sbjct: 64  RVVENYSLLQNQVIRLMFKSR-KRKAGGDSCNFNRSG------SDQYCGCCSDDDSCYKR 123

Query: 124 PRENSKPKVKRVLVPTPLSDSTLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCPVK 183
           PRE+SKPKV RVLV TP+SDS+LVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCPVK
Sbjct: 124 PRESSKPKVMRVLVSTPVSDSSLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCPVK 183

Query: 184 KKVQRSAQDPSFLVATYEGEHNHMQPNSGIEYQLVGPIQLGSNKLESSVSSPPS------ 243
           +KVQRS +DPS+LVATYEGEHNH +PNSGIEYQLVGPIQLGSN L+SSVSSP S      
Sbjct: 184 RKVQRSVEDPSYLVATYEGEHNHPKPNSGIEYQLVGPIQLGSN-LDSSVSSPSSSVVKGP 243

Query: 244 ----------MRSPSSSSVDTLKASLP---------STPQILVQQMASFLTRDPNFTTAL 289
                       S S S+ +T+KAS+P         ST  ILVQQMAS LTRDPNFT AL
Sbjct: 244 SIMPSVVTYDYLSKSPSTTNTVKASVPELETPPASSSTQTILVQQMASLLTRDPNFTRAL 303

BLAST of MS001356 vs. ExPASy TrEMBL
Match: A0A6J1GGZ9 (probable WRKY transcription factor 40 OS=Cucurbita moschata OX=3662 GN=LOC111454104 PE=4 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 1.4e-103
Identity = 215/306 (70.26%), Postives = 247/306 (80.72%), Query Frame = 0

Query: 4   SATINTSIDLNFNPPPPTADDSPANHTIYSSLKEDQAAGVLVEKLNRISSENAKLNQIIG 63
           S  INTS+ LNFNPPP T D SPAN ++  +  +++A GV+VEKLNRISSEN KLNQ++G
Sbjct: 4   SPAINTSLGLNFNPPPYTDDGSPANISVTPT--KEEAPGVIVEKLNRISSENQKLNQMLG 63

Query: 64  LVVENYSVLRNQVSDLMIKSRSKRKAGCDNCN-SNRSGNN-SNVFTDQYCGCCSDDDSCY 123
           +VVENYSVLRNQV DLMIKSR KRKAGCDNCN  NRSG++ ++  TDQYC C SDDDSC+
Sbjct: 64  VVVENYSVLRNQVVDLMIKSR-KRKAGCDNCNFFNRSGSSGADALTDQYCCCGSDDDSCH 123

Query: 124 KRPRENSKPKVKRVLVPTPLSDSTLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCP 183
           KRPRE+S+PK+KRVLV TP+SDS+L VKDGYQWRKYGQKVTK NPSPRAYYKCSFAPSCP
Sbjct: 124 KRPRESSRPKIKRVLVQTPISDSSLAVKDGYQWRKYGQKVTKGNPSPRAYYKCSFAPSCP 183

Query: 184 VKKKVQRSAQDPSFLVATYEGEHNHMQPNSGIEYQLVGPIQLGSNKLESSVSSP-PSMRS 243
           VKKKVQRS QD S+LVATYEGEHNH +PNSGIEYQLVGPI LGS KL+SSVSSP  S+ S
Sbjct: 184 VKKKVQRSVQDSSYLVATYEGEHNHKKPNSGIEYQLVGPIHLGS-KLDSSVSSPSSSVMS 243

Query: 244 PS-----------SSSVDTLKASLP-------STPQILVQQMASFLTRDPNFTTALATAI 289
           PS           S S+DTLK+ +P       ST +++ QQMAS LTRDPNFT AL TAI
Sbjct: 244 PSLPSITTFDLTKSQSIDTLKSPIPEFERPSTSTQRMVCQQMASLLTRDPNFTKALVTAI 303

BLAST of MS001356 vs. ExPASy TrEMBL
Match: A0A6J1KLB2 (probable WRKY transcription factor 40 OS=Cucurbita maxima OX=3661 GN=LOC111496280 PE=4 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.9e-103
Identity = 215/306 (70.26%), Postives = 246/306 (80.39%), Query Frame = 0

Query: 4   SATINTSIDLNFNPPPPTADDSPANHTIYSSLKEDQAAGVLVEKLNRISSENAKLNQIIG 63
           S  INTS+ LNFNPPP T D SPAN ++  +  +++A  VL EKLNRISSEN KLNQ++G
Sbjct: 4   SPAINTSLGLNFNPPPYTDDGSPANISVTPT--KEEAPAVLAEKLNRISSENQKLNQMLG 63

Query: 64  LVVENYSVLRNQVSDLMIKSRSKRKAGCDNCN-SNRSGNN-SNVFTDQYCGCCSDDDSCY 123
           +VVENYSVLRNQ+ DLMIKSR KRKAGCDNCN  NRSG++ ++  TDQYC C SDDDSC+
Sbjct: 64  VVVENYSVLRNQMVDLMIKSR-KRKAGCDNCNFFNRSGSSGADALTDQYCCCGSDDDSCH 123

Query: 124 KRPRENSKPKVKRVLVPTPLSDSTLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCP 183
           KRPRE+S+PK+KRVLV TP+SDS+L VKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCP
Sbjct: 124 KRPRESSRPKIKRVLVQTPISDSSLAVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCP 183

Query: 184 VKKKVQRSAQDPSFLVATYEGEHNHMQPNSGIEYQLVGPIQLGSNKLESSVSSPPS-MRS 243
           VKKKVQRS QD S+LVATYEGEHNH +PNSGIEYQLVGPI LGS KL+SSVSSP S + S
Sbjct: 184 VKKKVQRSVQDSSYLVATYEGEHNHKKPNSGIEYQLVGPIHLGS-KLDSSVSSPSSLLMS 243

Query: 244 PS-----------SSSVDTLKASLP-------STPQILVQQMASFLTRDPNFTTALATAI 289
           PS           S S+DTLK+ +P       ST Q++ QQMAS LTRDPNFT AL TAI
Sbjct: 244 PSLPSVTTFDLTKSQSIDTLKSLVPELERPSTSTQQMVFQQMASLLTRDPNFTKALVTAI 303

BLAST of MS001356 vs. TAIR 10
Match: AT1G80840.1 (WRKY DNA-binding protein 40 )

HSP 1 Score: 193.0 bits (489), Expect = 3.4e-49
Identity = 125/304 (41.12%), Postives = 175/304 (57.57%), Query Frame = 0

Query: 3   ASATINTSIDLNFNPPPPTADDSPANHTIYSSLKEDQAAGVLVEKLNRISSENAKLNQII 62
           +S+ ++TS+DL                     ++ED     LVE+LNR+S+EN KL++++
Sbjct: 5   SSSLVDTSLDLTIG-------------VTRMRVEEDPPTSALVEELNRVSAENKKLSEML 64

Query: 63  GLVVENYSVLRNQVSDLMIKSRSKRKAGCDNCNSNRSGNNSNVFTDQYCGCCSD------ 122
            L+ +NY+VLR Q+ + + KS    +         +S    + F+    G  S+      
Sbjct: 65  TLMCDNYNVLRKQLMEYVNKSNITERDQISPPKKRKSPAREDAFSCAVIGGVSESSSTDQ 124

Query: 123 DDSCYKRPRENS--KPKVKRVLVPTPLSDSTLVVKDGYQWRKYGQKVTKDNPSPRAYYKC 182
           D+   K+ RE +  K KV RV   T  SD+TLVVKDGYQWRKYGQKVT+DNPSPRAY+KC
Sbjct: 125 DEYLCKKQREETVVKEKVSRVYYKTEASDTTLVVKDGYQWRKYGQKVTRDNPSPRAYFKC 184

Query: 183 SFAPSCPVKKKVQRSAQDPSFLVATYEGEHNHMQP-----NSGIEYQL----VGPIQLGS 242
           + APSC VKKKVQRS +D S LVATYEGEHNH  P     N+G+   +         + +
Sbjct: 185 ACAPSCSVKKKVQRSVEDQSVLVATYEGEHNHPMPSQIDSNNGLNRHISHGGSASTPVAA 244

Query: 243 NKLESSVSSPPS---MRSPSSSSVDTLKASLPSTPQILVQQMASFLTRDPNFTTALATAI 287
           N+  SS++ P +   M      +  T +   P   ++LV+QMAS LT+DPNFT ALA A+
Sbjct: 245 NR-RSSLTVPVTTVDMIESKKVTSPTSRIDFPQVQKLLVEQMASSLTKDPNFTAALAAAV 294

BLAST of MS001356 vs. TAIR 10
Match: AT4G31800.1 (WRKY DNA-binding protein 18 )

HSP 1 Score: 182.2 bits (461), Expect = 6.0e-46
Identity = 127/313 (40.58%), Postives = 174/313 (55.59%), Query Frame = 0

Query: 4   SATINTSIDLNFNP-----PPPTADDSPANHTIYSSLKEDQAAGVLVEKLNRISSENAKL 63
           S+ ++ S+DLN NP     P        + H     L++D++A  L E+LNR++SEN KL
Sbjct: 4   SSFLDISLDLNTNPFSAKLPKKEVSVLASTHLKRKWLEQDESASELREELNRVNSENKKL 63

Query: 64  NQIIGLVVENYSVLRNQVSDLMIKSR----------SKRKAGCDNC-----------NSN 123
            +++  V E+Y+ L N +  L  +             KRK   D               N
Sbjct: 64  TEMLARVCESYNELHNHLEKLQSRQSPEIEQTDIPIKKRKQDPDEFLGFPIGLSSGKTEN 123

Query: 124 RSGNNSNVFTDQYCGCCSDDDSCYKRPRENS--KPKVKRVLVPTPLSDSTLVVKDGYQWR 183
            S N  +    Q     +   SC KRP  +S  K KV  V VPT  SD++L VKDG+QWR
Sbjct: 124 SSSNEDHHHHHQQHEQKNQLLSC-KRPVTDSFNKAKVSTVYVPTETSDTSLTVKDGFQWR 183

Query: 184 KYGQKVTKDNPSPRAYYKCSFAPSCPVKKKVQRSAQDPSFLVATYEGEHNHMQPNSGIEY 243
           KYGQKVT+DNPSPRAY++CSFAPSCPVKKKVQRSA+DPS LVATYEG HNH+ PN+    
Sbjct: 184 KYGQKVTRDNPSPRAYFRCSFAPSCPVKKKVQRSAEDPSLLVATYEGTHNHLGPNAS--- 243

Query: 244 QLVGPIQLGSNKLESSVSSPPSMRSPSSSSVDTLKASLPSTPQILVQQMASFLTRDPNFT 289
           +     Q GS+ +   + +     +   +  D       +  ++L+QQMAS LT+D  FT
Sbjct: 244 EGDATSQGGSSTVTLDLVNGCHRLALEKNERDN------TMQEVLIQQMASSLTKDSKFT 303

BLAST of MS001356 vs. TAIR 10
Match: AT4G31800.2 (WRKY DNA-binding protein 18 )

HSP 1 Score: 181.8 bits (460), Expect = 7.8e-46
Identity = 127/312 (40.71%), Postives = 174/312 (55.77%), Query Frame = 0

Query: 4   SATINTSIDLNFNP----PPPTADDSPANHTIYSSLKEDQAAGVLVEKLNRISSENAKLN 63
           S+ ++ S+DLN NP     P       + H     L++D++A  L E+LNR++SEN KL 
Sbjct: 4   SSFLDISLDLNTNPFSAKLPKEVSVLASTHLKRKWLEQDESASELREELNRVNSENKKLT 63

Query: 64  QIIGLVVENYSVLRNQVSDLMIKSR----------SKRKAGCDNC-----------NSNR 123
           +++  V E+Y+ L N +  L  +             KRK   D               N 
Sbjct: 64  EMLARVCESYNELHNHLEKLQSRQSPEIEQTDIPIKKRKQDPDEFLGFPIGLSSGKTENS 123

Query: 124 SGNNSNVFTDQYCGCCSDDDSCYKRPRENS--KPKVKRVLVPTPLSDSTLVVKDGYQWRK 183
           S N  +    Q     +   SC KRP  +S  K KV  V VPT  SD++L VKDG+QWRK
Sbjct: 124 SSNEDHHHHHQQHEQKNQLLSC-KRPVTDSFNKAKVSTVYVPTETSDTSLTVKDGFQWRK 183

Query: 184 YGQKVTKDNPSPRAYYKCSFAPSCPVKKKVQRSAQDPSFLVATYEGEHNHMQPNSGIEYQ 243
           YGQKVT+DNPSPRAY++CSFAPSCPVKKKVQRSA+DPS LVATYEG HNH+ PN+    +
Sbjct: 184 YGQKVTRDNPSPRAYFRCSFAPSCPVKKKVQRSAEDPSLLVATYEGTHNHLGPNAS---E 243

Query: 244 LVGPIQLGSNKLESSVSSPPSMRSPSSSSVDTLKASLPSTPQILVQQMASFLTRDPNFTT 289
                Q GS+ +   + +     +   +  D       +  ++L+QQMAS LT+D  FT 
Sbjct: 244 GDATSQGGSSTVTLDLVNGCHRLALEKNERDN------TMQEVLIQQMASSLTKDSKFTA 303

BLAST of MS001356 vs. TAIR 10
Match: AT2G25000.1 (WRKY DNA-binding protein 60 )

HSP 1 Score: 168.7 bits (426), Expect = 6.8e-42
Identity = 110/283 (38.87%), Postives = 157/283 (55.48%), Query Frame = 0

Query: 12  DLNFNPPPPTADDSPANHTIYSS--LKEDQAAGVLVEKLNRISSENAKLNQIIGLVVENY 71
           DL+F+   P  + S +   +     L +D+   +L +++NR++SEN KL +++  V E Y
Sbjct: 11  DLHFSGKLPKREVSASASKVVEKKWLVKDEKRNMLQDEINRVNSENKKLTEMLARVCEKY 70

Query: 72  SVLRNQVSDLMIKSRSKRKAGCDNCNSNRSGNNSNVFTDQYCGCCSDDDSCYKRPRE--- 131
             L N    LM + +S++     N  + +         D++    S        P E   
Sbjct: 71  YALNN----LMEELQSRKSPESVNFQNKQLTGKRKQELDEF---VSSPIGLSLGPIENIT 130

Query: 132 NSKPKVKRVLVPTPLSDSTLVVKDGYQWRKYGQKVTKDNPSPRAYYKCSFAPSCPVKKKV 191
           N K  V         SD++L VKDGYQWRKYGQK+T+DNPSPRAY++CSF+PSC VKKKV
Sbjct: 131 NDKATVSTAYFAAEKSDTSLTVKDGYQWRKYGQKITRDNPSPRAYFRCSFSPSCLVKKKV 190

Query: 192 QRSAQDPSFLVATYEGEHNHMQPNSGIEYQL-VGPIQLGSNKLESSVSSPPSMRSPSSSS 251
           QRSA+DPSFLVATYEG HNH  P++ +   + +  +Q G   +E                
Sbjct: 191 QRSAEDPSFLVATYEGTHNHTGPHASVSRTVKLDLVQGGLEPVEE--------------- 250

Query: 252 VDTLKASLPSTPQILVQQMASFLTRDPNFTTALATAITGTMVD 289
               K    +  ++LVQQMAS LT+DP FT ALATAI+G +++
Sbjct: 251 ----KKERGTIQEVLVQQMASSLTKDPKFTAALATAISGRLIE 267

BLAST of MS001356 vs. TAIR 10
Match: AT1G18860.1 (WRKY DNA-binding protein 61 )

HSP 1 Score: 97.1 bits (240), Expect = 2.5e-20
Identity = 56/153 (36.60%), Postives = 80/153 (52.29%), Query Frame = 0

Query: 101 NNSNVFTDQYCGCCSDDDSCYKRPRENSKPKVKRVLVPTPLSDSTLVVKDGYQWRKYGQK 160
           NN+ + +    G  +D D          +  VK+  V       T  + DG QWRKYGQK
Sbjct: 143 NNNKIRSQNSFGFKNDGDDHEDEDEILPQNLVKKTRVSVRSRCETPTMNDGCQWRKYGQK 202

Query: 161 VTKDNPSPRAYYKCSFAPSCPVKKKVQRSAQDPSFLVATYEGEHNHMQPNSGIEYQLVGP 220
           + K NP PRAYY+C+ A SCPV+K+VQR ++D S L++TYEG HNH             P
Sbjct: 203 IAKGNPCPRAYYRCTIAASCPVRKQVQRCSEDMSILISTYEGTHNH-------------P 262

Query: 221 IQLGSNKLESSVSSPPSMRSPSSSSVDTLKASL 254
           + + +  + S+ S+  SM    +SS  +  A L
Sbjct: 263 LPMSATAMASATSAAASMLLSGASSSSSAAADL 282

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022131459.13.9e-15699.31probable WRKY transcription factor 40 [Momordica charantia][more]
XP_023545515.11.4e-10571.29probable WRKY transcription factor 40 [Cucurbita pepo subsp. pepo][more]
KAG6598546.14.1e-10570.97putative WRKY transcription factor 40, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022996454.12.0e-10470.97probable WRKY transcription factor 40 [Cucurbita maxima][more]
XP_022961730.12.6e-10470.97probable WRKY transcription factor 40 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SAH74.8e-4841.12Probable WRKY transcription factor 40 OS=Arabidopsis thaliana OX=3702 GN=WRKY40 ... [more]
Q9C5T48.4e-4540.58WRKY transcription factor 18 OS=Arabidopsis thaliana OX=3702 GN=WRKY18 PE=1 SV=2[more]
Q9SK339.6e-4138.87Probable WRKY transcription factor 60 OS=Arabidopsis thaliana OX=3702 GN=WRKY60 ... [more]
Q6IEL03.1e-3936.54WRKY transcription factor WRKY71 OS=Oryza sativa subsp. indica OX=39946 GN=WRKY7... [more]
Q6QHD13.1e-3936.54WRKY transcription factor WRKY71 OS=Oryza sativa subsp. japonica OX=39947 GN=WRK... [more]
Match NameE-valueIdentityDescription
A0A6J1BQA81.9e-15699.31probable WRKY transcription factor 40 OS=Momordica charantia OX=3673 GN=LOC11100... [more]
A0A6J1K8R99.8e-10570.97probable WRKY transcription factor 40 OS=Cucurbita maxima OX=3661 GN=LOC11149169... [more]
A0A6J1HD491.3e-10470.97probable WRKY transcription factor 40 OS=Cucurbita moschata OX=3662 GN=LOC111462... [more]
A0A6J1GGZ91.4e-10370.26probable WRKY transcription factor 40 OS=Cucurbita moschata OX=3662 GN=LOC111454... [more]
A0A6J1KLB21.9e-10370.26probable WRKY transcription factor 40 OS=Cucurbita maxima OX=3661 GN=LOC11149628... [more]
Match NameE-valueIdentityDescription
AT1G80840.13.4e-4941.12WRKY DNA-binding protein 40 [more]
AT4G31800.16.0e-4640.58WRKY DNA-binding protein 18 [more]
AT4G31800.27.8e-4640.71WRKY DNA-binding protein 18 [more]
AT2G25000.16.8e-4238.87WRKY DNA-binding protein 60 [more]
AT1G18860.12.5e-2036.60WRKY DNA-binding protein 61 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 148..208
e-value: 1.1E-34
score: 131.2
IPR003657WRKY domainPFAMPF03106WRKYcoord: 149..206
e-value: 1.5E-24
score: 86.0
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 143..209
score: 27.473515
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 134..210
e-value: 4.1E-30
score: 105.4
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 145..209
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 226..250
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..16
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..27
NoneNo IPR availablePANTHERPTHR31429:SF3WRKY TRANSCRIPTION FACTOR 40-RELATEDcoord: 5..287
IPR044810WRKY transcription factor, plantPANTHERPTHR31429WRKY TRANSCRIPTION FACTOR 36-RELATEDcoord: 5..287

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS001356.1MS001356.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding