MS001289 (gene) Bitter gourd (TR) v1

Overview
NameMS001289
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionAmmonium transporter
Locationscaffold36: 2997170 .. 2999990 (+)
RNA-Seq ExpressionMS001289
SyntenyMS001289
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTAACTATTCGATCATAGTTCCTCTACCAGCGAATCTATTGGAAGACGCAGCGGACCCGGGATGGATGAGCAAGGGCGACAACGCGTGGCAGATGACGGCGGCGACGCTGGTGGGGCTGCAGAGCGTGCCGGGGCTGATAATCCTGTACGGCGGGGCGGTGAAGAAGAAGTGGGCGATCAACTCGGCGTTCATGGCGCTGTACGCGTTCGCGTGCGTGCTGGTGTGCTGGGTCGGGTGGGGGTACCGGCTGTCGTTTGGGGACAAGCTGGTGCCGTTTTGGGGGAAGCTCAACGTGGCGCTGGACCAGAAGTTTCTGCTGGACCCGGCCTTCAGCGGCAAGTTCCCCTCCGCGACCATGGTGTTCTTCCAGTTCGTGTTCGCCGCGATCACTCTGGTCCTCATCGCGGGGGCTCTCCTGGGCCGGATGAATTTCTACGCTTGGATGCTCTTCGTGCCGCTCTGGCTCACCTTCTCCTATACCTTTGTGGCGTTCAGTATTTGGAGCCCTAATGGGTTCTTCTATAAGATGGGGCTCATTGATTATTCCGGTGGCTATGTCATTCATTTGTCCTCTGGAGTTGCCGGCTTCACTGCCGCTTACTGGGTACGCCCTTTGACTCTTCGTCCACCCTTCCAAATTTGATAGCTACTTTATTTAAAAATATATATATATTCAAATACCGGACGGTGAAGATAAGATTTGATCCTCACAATAGTAATTGTTCTTACCACTATTTATCAATTTTTAAGGGCGGTGAAGATAAGATTTGGTCCTCGAATCCATACTGATTAATTAAGTTTATTTTTTTTCCCATACTTTTCTTTTAAAAAGAGGAACAATCTCTCTTATTTCTCCTTTTATATATTTTTCTTTCCTAATTTTAAAAAACACAATGGTGTTTATTAGTAGGAGATATTCACTAATTATTCAGCAAGTAAGTATTTTTTTTTTTTTTTGAGAAGCAGCAAGTATTATTAGAAGACTATTTGGTCCGTAAATTTTTAGAAAGGTTGATTATATTCATCTCTAAATTTATTTTGGTCTATTTTTGTTGTTTAATATCTTTGTTTATTGATATCCTTTATGGTCAACTCCCGTGCTGTTTTTTTTTTTCAAATGAATTATAAAATATAAGCCTAGTGTAAAATTGACAAGGCTTCGAAGACAAATATGACACTAGAACTACTTTAGTTCAAATTTTATAATTTGAGAGATTTGTTTAATGGTTTATTTTGTATATTAGTGATCGAGCTTAATTTTGCAAAAGAGTGATTTTCATTGAAGTGAGTGTTTTTAAAAAGATTTAGACATTGATTTTTTTATTAAAATTGATGGGCAAATGAAATAATTTAGATAAAAGGTAAAAATTGTTGCGTCACATGTCTAAATCTATGATCTAGAATGATGAAATTAGGAGTGTATAGTAAAAACTGATACATCCTATAGTAAATATTGTTTTTTATTTTCTTGTAAAATTAAAACATTTTTTATTAACTATAACATAAGTAGGAGACAAGAAGAAAAAAAAAGACGTAAAATTTTGAGAATTTGTTTTAGAAAATAGAAACCTATATACATATATTTCTCAAAACATAAAAACTAACACATAATGAGAAACATCAAAAGAGACGAAAACTTTGCCATGCTAAAAATAATAGGACACAATAATAAAACTTAGAGGTGCGTATCTTCTAACTCACAATTGTTATAAAATTATTGACAAATGTTTATTTTATTCAAAAAGGGACAATTGTTTATTTTATTAAAAATTTGACGAGATTTTTTTTATTTTCTCTCCATTTCTTTCATGCATTCATATCTTTGATATCAATTTTTTTAATAGTGATAAATAAGAAATATCTACTTATCAATAAATATTTAAGTTTGGCATAAAAAACTATTAAAGAAAAATAAACTTGTTTTTAAAAAAGAAAAAAATTTAAAATAATCTTGAAAATACTTAAAAGTTCTTACCATCTCCCTATACGGACTATCATTGAATGTGTATTAGGTTGGCCCACGACTCTCGAAAGATCGGGAGAGCTTCCCACCGAACAACATCCTTCTAATGCTGGCGGGAGCCGGGTTACTATGGATGGGGTGGACCGGGTTCAACGGCGGAGACCCTTATGCGGCCAACATCGACGCCTCCCTCGCGGTACTCAACACCCACGTCTGCACCGCGACCAGCCTGCTGACATGGCTGATCTTCGACATCGTGTTCTTCCGTAAACCCTCGGTGATCGGCGCTGTCCAAGGCATGATCACCGGCCTGGTATGCATCACCCCGGCCGCCGGCCTGGTTCAAGGATGGGCCGCCATAATCATGGGAATGCTATCAGGGTCCATCCCGTGGTTCACCATGATGGTGGTCCACAAGAAATCCCCGGTCCTCCAAAAGGTGGACGACACAATGGCCGTGTTCCACACCCACGCCATCGCGGGCAGCCTCGGCGGCATCCTCAGCGGCATCTTCGCCGAGCCGCGCCTGAACTCCATCTACTACGGCGCGTACAAAAAATACGTGGGCCTGTTCTACGGATTCAGCACGGCCCAAGTGGGGCTCGGCTTCCGCCAACTCGGCGTCCAACTCCTCGGCATCATATTTGTGACGTTGATCAATGTCATCGTCACCACCATCATCTGTGTGGCTCTCCAGTACATCGTGCCGCTACGGATGACGGAGGAGGACATGGCCATCGGCGACGAGGCCGCACACGGCGAAGAGGCTTACGCCATCTGGGGGCAGGGGGAGAAGCTTGATAACTCTAAGTATGACATTGAGATGCCTGCAAAGCACAGACACGCAGCTGAA

mRNA sequence

ATGAGTAACTATTCGATCATAGTTCCTCTACCAGCGAATCTATTGGAAGACGCAGCGGACCCGGGATGGATGAGCAAGGGCGACAACGCGTGGCAGATGACGGCGGCGACGCTGGTGGGGCTGCAGAGCGTGCCGGGGCTGATAATCCTGTACGGCGGGGCGGTGAAGAAGAAGTGGGCGATCAACTCGGCGTTCATGGCGCTGTACGCGTTCGCGTGCGTGCTGGTGTGCTGGGTCGGGTGGGGGTACCGGCTGTCGTTTGGGGACAAGCTGGTGCCGTTTTGGGGGAAGCTCAACGTGGCGCTGGACCAGAAGTTTCTGCTGGACCCGGCCTTCAGCGGCAAGTTCCCCTCCGCGACCATGGTGTTCTTCCAGTTCGTGTTCGCCGCGATCACTCTGGTCCTCATCGCGGGGGCTCTCCTGGGCCGGATGAATTTCTACGCTTGGATGCTCTTCGTGCCGCTCTGGCTCACCTTCTCCTATACCTTTGTGGCGTTCAGTATTTGGAGCCCTAATGGGTTCTTCTATAAGATGGGGCTCATTGATTATTCCGGTGGCTATGTCATTCATTTGTCCTCTGGAGTTGCCGGCTTCACTGCCGCTTACTGGGTTGGCCCACGACTCTCGAAAGATCGGGAGAGCTTCCCACCGAACAACATCCTTCTAATGCTGGCGGGAGCCGGGTTACTATGGATGGGGTGGACCGGGTTCAACGGCGGAGACCCTTATGCGGCCAACATCGACGCCTCCCTCGCGGTACTCAACACCCACGTCTGCACCGCGACCAGCCTGCTGACATGGCTGATCTTCGACATCGTGTTCTTCCGTAAACCCTCGGTGATCGGCGCTGTCCAAGGCATGATCACCGGCCTGGTATGCATCACCCCGGCCGCCGGCCTGGTTCAAGGATGGGCCGCCATAATCATGGGAATGCTATCAGGGTCCATCCCGTGGTTCACCATGATGGTGGTCCACAAGAAATCCCCGGTCCTCCAAAAGGTGGACGACACAATGGCCGTGTTCCACACCCACGCCATCGCGGGCAGCCTCGGCGGCATCCTCAGCGGCATCTTCGCCGAGCCGCGCCTGAACTCCATCTACTACGGCGCGTACAAAAAATACGTGGGCCTGTTCTACGGATTCAGCACGGCCCAAGTGGGGCTCGGCTTCCGCCAACTCGGCGTCCAACTCCTCGGCATCATATTTGTGACGTTGATCAATGTCATCGTCACCACCATCATCTGTGTGGCTCTCCAGTACATCGTGCCGCTACGGATGACGGAGGAGGACATGGCCATCGGCGACGAGGCCGCACACGGCGAAGAGGCTTACGCCATCTGGGGGCAGGGGGAGAAGCTTGATAACTCTAAGTATGACATTGAGATGCCTGCAAAGCACAGACACGCAGCTGAA

Coding sequence (CDS)

ATGAGTAACTATTCGATCATAGTTCCTCTACCAGCGAATCTATTGGAAGACGCAGCGGACCCGGGATGGATGAGCAAGGGCGACAACGCGTGGCAGATGACGGCGGCGACGCTGGTGGGGCTGCAGAGCGTGCCGGGGCTGATAATCCTGTACGGCGGGGCGGTGAAGAAGAAGTGGGCGATCAACTCGGCGTTCATGGCGCTGTACGCGTTCGCGTGCGTGCTGGTGTGCTGGGTCGGGTGGGGGTACCGGCTGTCGTTTGGGGACAAGCTGGTGCCGTTTTGGGGGAAGCTCAACGTGGCGCTGGACCAGAAGTTTCTGCTGGACCCGGCCTTCAGCGGCAAGTTCCCCTCCGCGACCATGGTGTTCTTCCAGTTCGTGTTCGCCGCGATCACTCTGGTCCTCATCGCGGGGGCTCTCCTGGGCCGGATGAATTTCTACGCTTGGATGCTCTTCGTGCCGCTCTGGCTCACCTTCTCCTATACCTTTGTGGCGTTCAGTATTTGGAGCCCTAATGGGTTCTTCTATAAGATGGGGCTCATTGATTATTCCGGTGGCTATGTCATTCATTTGTCCTCTGGAGTTGCCGGCTTCACTGCCGCTTACTGGGTTGGCCCACGACTCTCGAAAGATCGGGAGAGCTTCCCACCGAACAACATCCTTCTAATGCTGGCGGGAGCCGGGTTACTATGGATGGGGTGGACCGGGTTCAACGGCGGAGACCCTTATGCGGCCAACATCGACGCCTCCCTCGCGGTACTCAACACCCACGTCTGCACCGCGACCAGCCTGCTGACATGGCTGATCTTCGACATCGTGTTCTTCCGTAAACCCTCGGTGATCGGCGCTGTCCAAGGCATGATCACCGGCCTGGTATGCATCACCCCGGCCGCCGGCCTGGTTCAAGGATGGGCCGCCATAATCATGGGAATGCTATCAGGGTCCATCCCGTGGTTCACCATGATGGTGGTCCACAAGAAATCCCCGGTCCTCCAAAAGGTGGACGACACAATGGCCGTGTTCCACACCCACGCCATCGCGGGCAGCCTCGGCGGCATCCTCAGCGGCATCTTCGCCGAGCCGCGCCTGAACTCCATCTACTACGGCGCGTACAAAAAATACGTGGGCCTGTTCTACGGATTCAGCACGGCCCAAGTGGGGCTCGGCTTCCGCCAACTCGGCGTCCAACTCCTCGGCATCATATTTGTGACGTTGATCAATGTCATCGTCACCACCATCATCTGTGTGGCTCTCCAGTACATCGTGCCGCTACGGATGACGGAGGAGGACATGGCCATCGGCGACGAGGCCGCACACGGCGAAGAGGCTTACGCCATCTGGGGGCAGGGGGAGAAGCTTGATAACTCTAAGTATGACATTGAGATGCCTGCAAAGCACAGACACGCAGCTGAA

Protein sequence

MSNYSIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLNSIYYGAYKKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPLRMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMPAKHRHAAE
Homology
BLAST of MS001289 vs. NCBI nr
Match: XP_022131630.1 (ammonium transporter 3 member 1 [Momordica charantia])

HSP 1 Score: 942.2 bits (2434), Expect = 1.7e-270
Identity = 470/471 (99.79%), Postives = 470/471 (99.79%), Query Frame = 0

Query: 1   MSNYSIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWA 60
           MSNYSIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWA
Sbjct: 1   MSNYSIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWA 60

Query: 61  INSAFMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSAT 120
           INSAFMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSAT
Sbjct: 61  INSAFMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSAT 120

Query: 121 MVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGL 180
           MVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGL
Sbjct: 121 MVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGL 180

Query: 181 IDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGG 240
           IDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGG
Sbjct: 181 IDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGG 240

Query: 241 DPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGL 300
           DPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGL
Sbjct: 241 DPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGL 300

Query: 301 VQGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAE 360
           VQGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAE
Sbjct: 301 VQGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAE 360

Query: 361 PRLNSIYYGAYKKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQY 420
           PRLNSIYYGAY KYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQY
Sbjct: 361 PRLNSIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQY 420

Query: 421 IVPLRMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMPAKHRHAAE 472
           IVPLRMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMPAKHRHAAE
Sbjct: 421 IVPLRMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMPAKHRHAAE 471

BLAST of MS001289 vs. NCBI nr
Match: XP_022962592.1 (ammonium transporter 3 member 1-like [Cucurbita moschata] >KAG6598591.1 Ammonium transporter 3 member 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029524.1 Ammonium transporter 3 member 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 875.2 bits (2260), Expect = 2.6e-250
Identity = 432/467 (92.51%), Postives = 454/467 (97.22%), Query Frame = 0

Query: 5   SIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 64
           S+IVPLP+NLL D A+P WMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA
Sbjct: 2   SLIVPLPSNLLPDPANPEWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 61

Query: 65  FMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFF 124
           FMALYAFACVLVCWVGWGYRLSFGDKL+PFWGKLNVALDQ+FLL+ AFSGK PSATMVFF
Sbjct: 62  FMALYAFACVLVCWVGWGYRLSFGDKLLPFWGKLNVALDQQFLLEQAFSGKLPSATMVFF 121

Query: 125 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYS 184
           QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFF+KMGLIDYS
Sbjct: 122 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS 181

Query: 185 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 244
           GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA
Sbjct: 182 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 241

Query: 245 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGW 304
           ANIDASLAVLNTHVCTATSLLTWLIFDI+FFRKPSVIGAVQGMITGLVCITPAAG+VQGW
Sbjct: 242 ANIDASLAVLNTHVCTATSLLTWLIFDIIFFRKPSVIGAVQGMITGLVCITPAAGVVQGW 301

Query: 305 AAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 364
           AAIIMG+LSGSIPWFTMMVVHKKS +LQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN
Sbjct: 302 AAIIMGLLSGSIPWFTMMVVHKKSAILQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 361

Query: 365 SIYYGAYKKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPL 424
            +YYGAY KYVGLFYGF++ QV LGFRQLGVQLLGI+FVTLIN+IVT++ICV LQYIVPL
Sbjct: 362 FVYYGAYNKYVGLFYGFNSKQVKLGFRQLGVQLLGILFVTLINIIVTSLICVLLQYIVPL 421

Query: 425 RMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMP--AKHRHA 470
           RM+EEDMAIGDEAAHGEEAYAIWGQGEK+D+SKYDIE+P  AKHRHA
Sbjct: 422 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPVVAKHRHA 468

BLAST of MS001289 vs. NCBI nr
Match: XP_023546976.1 (ammonium transporter 3 member 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 874.4 bits (2258), Expect = 4.4e-250
Identity = 432/467 (92.51%), Postives = 453/467 (97.00%), Query Frame = 0

Query: 5   SIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 64
           S+IVPLP NLL D A+P WMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA
Sbjct: 2   SLIVPLPPNLLPDPANPEWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 61

Query: 65  FMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFF 124
           FMALYAFACVLVCWVGWGYRLSFGDKL+PFWGKLNVALDQ+FLL+ AFSGK PSATMVFF
Sbjct: 62  FMALYAFACVLVCWVGWGYRLSFGDKLLPFWGKLNVALDQQFLLEQAFSGKLPSATMVFF 121

Query: 125 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYS 184
           QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFF+KMGLIDYS
Sbjct: 122 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS 181

Query: 185 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 244
           GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA
Sbjct: 182 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 241

Query: 245 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGW 304
           ANIDASLAVLNTHVCTATSLLTWLIFDI+FFRKPSVIGAVQGMITGLVCITPAAG+VQGW
Sbjct: 242 ANIDASLAVLNTHVCTATSLLTWLIFDIIFFRKPSVIGAVQGMITGLVCITPAAGVVQGW 301

Query: 305 AAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 364
           AAIIMG+LSGSIPWFTMMVVHKKS +LQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN
Sbjct: 302 AAIIMGLLSGSIPWFTMMVVHKKSAILQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 361

Query: 365 SIYYGAYKKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPL 424
            +YYGAY KYVGLFYGF++ QV LGFRQLGVQLLGI+FVTLIN+IVT++ICV LQYIVPL
Sbjct: 362 FVYYGAYNKYVGLFYGFNSKQVKLGFRQLGVQLLGILFVTLINIIVTSLICVLLQYIVPL 421

Query: 425 RMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMP--AKHRHA 470
           RM+EEDMAIGDEAAHGEEAYAIWGQGEK+D+SKYDIE+P  AKHRHA
Sbjct: 422 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPVVAKHRHA 468

BLAST of MS001289 vs. NCBI nr
Match: XP_022996665.1 (ammonium transporter 3 member 1-like [Cucurbita maxima])

HSP 1 Score: 872.5 bits (2253), Expect = 1.7e-249
Identity = 431/469 (91.90%), Postives = 453/469 (96.59%), Query Frame = 0

Query: 5   SIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 64
           S+IVPLP+NLL D A+P WMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA
Sbjct: 2   SLIVPLPSNLLPDPANPEWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 61

Query: 65  FMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFF 124
           FMALYAFACVLVCWVGWGYRLSFGD+L+PFWGKLNVALDQ+FLL+ AFSGK PSATMVFF
Sbjct: 62  FMALYAFACVLVCWVGWGYRLSFGDRLLPFWGKLNVALDQQFLLEQAFSGKLPSATMVFF 121

Query: 125 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYS 184
           QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFF+KMGLIDYS
Sbjct: 122 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS 181

Query: 185 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 244
           GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA
Sbjct: 182 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 241

Query: 245 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGW 304
           ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAG+VQGW
Sbjct: 242 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVVQGW 301

Query: 305 AAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 364
           AAIIMG+LSGSIPWFTMMVVHKKS +LQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN
Sbjct: 302 AAIIMGLLSGSIPWFTMMVVHKKSAILQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 361

Query: 365 SIYYGAYKKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPL 424
            +YYGAY KYVGLFYGF++ QV LGFRQLGVQLLGI+FVTLIN+IVT++ICV LQYIVPL
Sbjct: 362 FVYYGAYNKYVGLFYGFNSKQVKLGFRQLGVQLLGILFVTLINIIVTSLICVLLQYIVPL 421

Query: 425 RMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMP--AKHRHAAE 472
           RM+EEDMAIGDEAAHGEEAYAIWGQGEK+D SKYDIE+P  AKHRH  +
Sbjct: 422 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDTSKYDIEVPAVAKHRHIGQ 470

BLAST of MS001289 vs. NCBI nr
Match: XP_004138867.1 (ammonium transporter 3 member 1 [Cucumis sativus] >KAE8652192.1 hypothetical protein Csa_021807 [Cucumis sativus])

HSP 1 Score: 867.1 bits (2239), Expect = 7.1e-248
Identity = 430/472 (91.10%), Postives = 451/472 (95.55%), Query Frame = 0

Query: 1   MSNYSIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWA 60
           MSN  IIVPLPANL+ D  DPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWA
Sbjct: 1   MSN--IIVPLPANLVTDVGDPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWA 60

Query: 61  INSAFMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSAT 120
           INSAFMALYAFACVLVCWVGWGYRLSFG+KL PFWGKLNVALDQ++LL P F+GKFP+AT
Sbjct: 61  INSAFMALYAFACVLVCWVGWGYRLSFGEKLAPFWGKLNVALDQEYLLAPGFAGKFPTAT 120

Query: 121 MVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGL 180
           MVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSP GFF KMGL
Sbjct: 121 MVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPGGFFAKMGL 180

Query: 181 IDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGG 240
           IDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGG
Sbjct: 181 IDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGG 240

Query: 241 DPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGL 300
           DPYAANIDASLAVLNTHVCTATSLLTWLIFDI+FFRKPSVIGAVQGMITGLVCITPAAG+
Sbjct: 241 DPYAANIDASLAVLNTHVCTATSLLTWLIFDIIFFRKPSVIGAVQGMITGLVCITPAAGV 300

Query: 301 VQGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAE 360
           VQGWAAIIMG+LSGSIPWFTMMVVHKKS +LQKVDDTMAV HTHAIAGSLGGILSGIFAE
Sbjct: 301 VQGWAAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAE 360

Query: 361 PRLNSIYYGAYKKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQY 420
           P LN+I+YG Y KY+GLFYGF+T QV LGFRQLGVQLLGIIFVTL+N+IVT++ICV LQY
Sbjct: 361 PHLNNIFYGNYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIIFVTLLNIIVTSLICVLLQY 420

Query: 421 IVPLRMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMP--AKHRHAA 471
           IVPLRM+EEDMAIGDEAAHGEEAYAIWGQGEK+D+SKYDIE P  AKHRHAA
Sbjct: 421 IVPLRMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEAPVSAKHRHAA 470

BLAST of MS001289 vs. ExPASy Swiss-Prot
Match: A0A072VHJ1 (Ammonium transporter 2 member 5 OS=Medicago truncatula OX=3880 GN=AMT2-5 PE=2 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 8.0e-210
Identity = 346/449 (77.06%), Postives = 395/449 (87.97%), Query Frame = 0

Query: 8   VPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMA 67
           VP P+NLL   + P W+SK DNAWQ+ AATLVG+QSVPGLIILYGGAVKKKWA+NSAFM+
Sbjct: 4   VPFPSNLLPSPSSPEWLSKADNAWQLMAATLVGMQSVPGLIILYGGAVKKKWAVNSAFMS 63

Query: 68  LYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFFQFV 127
           LYAFACV  CWV W YR+SFGD L PFWGK  +ALD+K+L   AF G FP+ATM++FQ V
Sbjct: 64  LYAFACVFFCWVFWAYRMSFGDTLFPFWGKPALALDEKYLFKQAFLGAFPNATMIYFQCV 123

Query: 128 FAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYSGGY 187
           FAAITL+LIAGA+LGRMNFYAWM+FVPLWLTFSYTF AFSIWS NGF  KMG+IDYSGGY
Sbjct: 124 FAAITLILIAGAVLGRMNFYAWMMFVPLWLTFSYTFTAFSIWSTNGFLAKMGIIDYSGGY 183

Query: 188 VIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYAANI 247
           VIHLSSGVAGFTAAYWVGPRL+KDRE FPPNN+LLMLAGAGLLWMGWTGFNGGDPY+  +
Sbjct: 184 VIHLSSGVAGFTAAYWVGPRLNKDRERFPPNNLLLMLAGAGLLWMGWTGFNGGDPYSVGL 243

Query: 248 DASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGWAAI 307
           DASLAVLNTH CTATSLLTW+  D++FFRKPSVIGAVQGMITGLVCITPAAG+VQGWAA+
Sbjct: 244 DASLAVLNTHACTATSLLTWVFLDVIFFRKPSVIGAVQGMITGLVCITPAAGVVQGWAAL 303

Query: 308 IMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLNSIY 367
           IMG+ SGSIPWFTMMV+HK+S +LQKVDDTMAV HTHAIAGSLGGIL+G+FAEP+LNS++
Sbjct: 304 IMGLFSGSIPWFTMMVIHKRSKLLQKVDDTMAVLHTHAIAGSLGGILTGLFAEPKLNSLF 363

Query: 368 YGAYKKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPLRMT 427
           YG Y KYVGLFYG        G RQ+G+QLLGI+FV  +NV+ T++IC+ ++  VPLRM+
Sbjct: 364 YGVYNKYVGLFYGIQMNMASTGRRQIGIQLLGILFVIFVNVVSTSLICLFIRLFVPLRMS 423

Query: 428 EEDMAIGDEAAHGEEAYAIWGQGEKLDNS 457
           EEDM IGDEAAHGEEAYAIWGQG+K +NS
Sbjct: 424 EEDMEIGDEAAHGEEAYAIWGQGDKHENS 452

BLAST of MS001289 vs. ExPASy Swiss-Prot
Match: Q84KJ6 (Ammonium transporter 3 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3-1 PE=2 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 6.2e-170
Identity = 298/463 (64.36%), Postives = 357/463 (77.11%), Query Frame = 0

Query: 19  ADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALYAFACVLVCW 78
           A P W++KGDNAWQM +ATLVG+QSVPGL+ILYG  VKKKWA+NSAFMALYAFA V +CW
Sbjct: 18  AVPEWLNKGDNAWQMISATLVGMQSVPGLVILYGSIVKKKWAVNSAFMALYAFAAVWLCW 77

Query: 79  VGWGYRLSFGDKLVPFWGKLNVALDQKFLL-----------------------DPAFSGK 138
           V WGY +SFG KL+PFWGK   AL Q FLL                        P  +  
Sbjct: 78  VTWGYNMSFGHKLLPFWGKARPALGQSFLLAQAVLPQTTQFYKGGGGADAVVETPWVNPL 137

Query: 139 FPSATMVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFF 198
           +P ATMV+FQ VFAAITL+L+AG+LLGRMN  AWMLFVPLWLTFSYT  AFS+W   GF 
Sbjct: 138 YPMATMVYFQCVFAAITLILLAGSLLGRMNIKAWMLFVPLWLTFSYTVGAFSLWG-GGFL 197

Query: 199 YKMGLIDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWT 258
           +  G++DYSGGYVIHLSSGVAGFTAAYWVGPR +KDRE FPPNN+LLML GAG+LWMGW 
Sbjct: 198 FHWGVMDYSGGYVIHLSSGVAGFTAAYWVGPRSTKDRERFPPNNVLLMLTGAGILWMGWA 257

Query: 259 GFNGGDPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCIT 318
           GFNGGDPY+ANID+SLAVLNT++C ATSLL W   D++FF+KPSVIGAVQGMITGLVCIT
Sbjct: 258 GFNGGDPYSANIDSSLAVLNTNICAATSLLVWTCLDVIFFKKPSVIGAVQGMITGLVCIT 317

Query: 319 PAAGLVQGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILS 378
           P AGLVQGWAAI+MG+LSGSIPWFTMMVVHK+S +LQ+VDDT+ VFHTHA+AG LGG  +
Sbjct: 318 PGAGLVQGWAAIVMGILSGSIPWFTMMVVHKRSRLLQQVDDTLGVFHTHAVAGFLGGATT 377

Query: 379 GIFAEPRLNSIYYGAYKKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIIC 438
           G+FAEP L S++          + G        G  Q   Q+ G +F+   NV+VT+++C
Sbjct: 378 GLFAEPVLCSLFLPVTNSRGAFYPGRG------GGLQFVRQVAGALFIICWNVVVTSLVC 437

Query: 439 VALQYIVPLRMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKY 459
           +A++ +VPLRM EE++AIGD+A HGEEAYA+WG GEK D++K+
Sbjct: 438 LAVRAVVPLRMPEEELAIGDDAVHGEEAYALWGDGEKYDSTKH 473

BLAST of MS001289 vs. ExPASy Swiss-Prot
Match: G7L1W7 (Ammonium transporter 2 member 4 OS=Medicago truncatula OX=3880 GN=AMT2-4 PE=2 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 1.8e-169
Identity = 291/453 (64.24%), Postives = 365/453 (80.57%), Query Frame = 0

Query: 10  LPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALY 69
           LP+NLL D A P WM+KGDNAWQ+TAAT+VGLQS+PGL+ILYG  VKK WAINSAFMA Y
Sbjct: 3   LPSNLLPDEASPEWMNKGDNAWQLTAATMVGLQSIPGLVILYGSLVKKTWAINSAFMAFY 62

Query: 70  AFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFFQFVFA 129
           AFA VL+CWV W Y++SFG+K+V F GK NVALD+KFLL  AF G FP+ATMVF+Q VFA
Sbjct: 63  AFASVLLCWVSWAYQMSFGEKMVFFLGKPNVALDEKFLLGKAFLGNFPNATMVFYQGVFA 122

Query: 130 AITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYSGGYVI 189
            +TL+LIAGALLGRMN  AWMLFVPLW+TFSYT VAFSIW P+G+  K G+ID++GGYVI
Sbjct: 123 GLTLILIAGALLGRMNIRAWMLFVPLWVTFSYTVVAFSIWCPDGWLAKRGVIDFAGGYVI 182

Query: 190 HLSSGVAGFTAAYWVGPRLSKDRESFP--PNNILLMLAGAGLLWMGWTGFNGGDPYAANI 249
           HLS+GVAGFTAAYWVGPR  KDRE+FP   NN++++LAGAGLLWMGW+GFNGG P+ A+ 
Sbjct: 183 HLSAGVAGFTAAYWVGPRADKDRETFPAATNNMIMVLAGAGLLWMGWSGFNGGAPFVAST 242

Query: 250 DASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGWAAI 309
            ASLA+LNTHVCTA S+  W++ D  +F KP+V GAVQGMITGLVCITPAAG+VQGWAAI
Sbjct: 243 IASLAILNTHVCTAASITVWVMLDTFYFGKPTVFGAVQGMITGLVCITPAAGVVQGWAAI 302

Query: 310 IMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLNSIY 369
           +MG +SGSIPW+TMMV+H K   L+K+DD MAVFHTHAIAG+LGGIL+G FA P+L  ++
Sbjct: 303 LMGFISGSIPWYTMMVLHNKVNFLKKIDDPMAVFHTHAIAGALGGILTGFFAVPKLCRLF 362

Query: 370 YGA--YKKYVGLFYGF-STAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPL 429
           Y    ++KY+GL YG  +      G +Q+ +Q+  I+FV   NV++T++IC+ ++ IVPL
Sbjct: 363 YMVPDWEKYIGLAYGLQNKGATQAGLKQMVIQIEAIVFVICYNVLMTSLICLIVRVIVPL 422

Query: 430 RMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSK 458
           R+  + + +GD+A HGE+A+A+  +  K  N K
Sbjct: 423 RLNGDALQMGDKAIHGEDAFALHSEATKFVNIK 455

BLAST of MS001289 vs. ExPASy Swiss-Prot
Match: Q69T29 (Ammonium transporter 3 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3-3 PE=2 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 3.7e-167
Identity = 288/459 (62.75%), Postives = 355/459 (77.34%), Query Frame = 0

Query: 19  ADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALYAFACVLVCW 78
           + P W++KGDNAWQMT+ATLVGLQS+PGL+ILYG  VKKKWAINSAFMALYAFA V +CW
Sbjct: 15  SSPDWLNKGDNAWQMTSATLVGLQSMPGLVILYGSIVKKKWAINSAFMALYAFAAVWICW 74

Query: 79  VGWGYRLSFGDKLVPFWGKLNVALDQKFLL------------------DPAFSGKFPSAT 138
           V W Y +SFGD+L+PFWGK   AL Q FL+                   P     +P AT
Sbjct: 75  VVWAYNMSFGDRLLPFWGKARPALGQSFLVAQSELTATAIRYHNGSAEAPMLKPLYPVAT 134

Query: 139 MVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGL 198
           MV+FQ +FA+IT++++AG+LLGRMN  AWM FVPLW+TFSYT  AFS+W   GF ++ G+
Sbjct: 135 MVYFQCMFASITIIILAGSLLGRMNIKAWMAFVPLWITFSYTVCAFSLWG-GGFLFQWGV 194

Query: 199 IDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGG 258
           IDYSGGYVIHLSSG+AG TAAYWVGPR + DRE FPPNNILL+LAGAGLLW+GWTGFNGG
Sbjct: 195 IDYSGGYVIHLSSGIAGLTAAYWVGPRSASDRERFPPNNILLVLAGAGLLWLGWTGFNGG 254

Query: 259 DPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGL 318
           DPY+ANID+S+AVLNTH+C +TSLL W I D+ FF KPSVIGAVQGMITGLVCITP AGL
Sbjct: 255 DPYSANIDSSMAVLNTHICASTSLLVWTILDVFFFGKPSVIGAVQGMITGLVCITPGAGL 314

Query: 319 VQGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAE 378
           VQGWAAI+MG+LSGSIPW+TMMV+HKK   +Q++DDT+ VFHTHA+AG LGG  +G+FAE
Sbjct: 315 VQGWAAIVMGILSGSIPWYTMMVLHKKWSFMQRIDDTLGVFHTHAVAGFLGGATTGLFAE 374

Query: 379 PRLNSIYYGAYKKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQY 438
           P L S++  +     G FYG      G G  Q G Q+ G +FVT  N+++T+IICV +  
Sbjct: 375 PILCSLFL-SIPDSKGAFYG------GPGGSQFGKQIAGALFVTAWNIVITSIICVIISL 434

Query: 439 IVPLRMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYD 460
           I+PLR+ ++++ IGD+A HGEEAYAIW +GE  D + ++
Sbjct: 435 ILPLRIADQELLIGDDAVHGEEAYAIWAEGELNDMTHHN 465

BLAST of MS001289 vs. ExPASy Swiss-Prot
Match: G7LAA8 (Ammonium transporter 2 member 3 OS=Medicago truncatula OX=3880 GN=AMT2-3 PE=2 SV=2)

HSP 1 Score: 589.0 bits (1517), Expect = 4.9e-167
Identity = 289/445 (64.94%), Postives = 350/445 (78.65%), Query Frame = 0

Query: 10  LPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALY 69
           LP +LL + A P W +K DNAWQ+TAATLVGLQ+VPGL+ILYG  VKKKWA+NSAFMALY
Sbjct: 12  LPESLLPNDASPEWNNKADNAWQLTAATLVGLQTVPGLVILYGSMVKKKWAVNSAFMALY 71

Query: 70  AFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKF-PSATMVFFQFVF 129
           AFA VLVCWV W + ++FG KL+PF GK N AL QKFLL  A +  + P A  VF+QF F
Sbjct: 72  AFAAVLVCWVLWAHHMAFGTKLLPFVGKPNFALSQKFLLSKASTNYYLPMADFVFYQFAF 131

Query: 130 AAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYSGGYV 189
           AAITLVL+ G+LLGRMNFYAWMLFVPLWLT SYT  AF+IW  NGF  +  +IDY+GG+V
Sbjct: 132 AAITLVLLGGSLLGRMNFYAWMLFVPLWLTLSYTVGAFTIWG-NGFL-EGKIIDYAGGFV 191

Query: 190 IHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYAANID 249
           IHLSSGVAGFTAAYWVGPR S DR++FPPNNI+ ML GAG LWMGWTGFNGG P+     
Sbjct: 192 IHLSSGVAGFTAAYWVGPRTSNDRQNFPPNNIIHMLGGAGFLWMGWTGFNGGAPFQVGEI 251

Query: 250 ASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGWAAII 309
            SLA+ NTH+CTATS+L W+  D+  ++K S+IG+VQGM+TGLVCITP AGLV  WAAI+
Sbjct: 252 TSLAIFNTHLCTATSILVWISLDMAVYKKGSLIGSVQGMMTGLVCITPGAGLVDPWAAIL 311

Query: 310 MGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLNSIYY 369
           MG LSGSIPW+TMMV+HKKSP  Q VDDT+ VFHTHA+AG LGGILSG+FA+P+L  I Y
Sbjct: 312 MGALSGSIPWYTMMVLHKKSPFFQSVDDTLGVFHTHAVAGILGGILSGVFAKPKLLRILY 371

Query: 370 GAYKKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPLRMTE 429
           G Y    GL Y +    +G G +Q+  QLLG +F+T+ NV++T++IC+ L   V LRM E
Sbjct: 372 GPYGS--GLLYSYFDDNIGQGIKQMWYQLLGAVFITIWNVVITSLICILLNRFVNLRMQE 431

Query: 430 EDMAIGDEAAHGEEAYAIWGQGEKL 454
           ED+ +GD+AAHGEEAY +WG GE++
Sbjct: 432 EDLEVGDDAAHGEEAYVLWGDGERM 452

BLAST of MS001289 vs. ExPASy TrEMBL
Match: A0A6J1BQ83 (Ammonium transporter OS=Momordica charantia OX=3673 GN=LOC111004759 PE=3 SV=1)

HSP 1 Score: 942.2 bits (2434), Expect = 8.4e-271
Identity = 470/471 (99.79%), Postives = 470/471 (99.79%), Query Frame = 0

Query: 1   MSNYSIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWA 60
           MSNYSIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWA
Sbjct: 1   MSNYSIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWA 60

Query: 61  INSAFMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSAT 120
           INSAFMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSAT
Sbjct: 61  INSAFMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSAT 120

Query: 121 MVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGL 180
           MVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGL
Sbjct: 121 MVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGL 180

Query: 181 IDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGG 240
           IDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGG
Sbjct: 181 IDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGG 240

Query: 241 DPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGL 300
           DPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGL
Sbjct: 241 DPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGL 300

Query: 301 VQGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAE 360
           VQGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAE
Sbjct: 301 VQGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAE 360

Query: 361 PRLNSIYYGAYKKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQY 420
           PRLNSIYYGAY KYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQY
Sbjct: 361 PRLNSIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQY 420

Query: 421 IVPLRMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMPAKHRHAAE 472
           IVPLRMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMPAKHRHAAE
Sbjct: 421 IVPLRMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMPAKHRHAAE 471

BLAST of MS001289 vs. ExPASy TrEMBL
Match: A0A6J1HD34 (Ammonium transporter OS=Cucurbita moschata OX=3662 GN=LOC111462995 PE=3 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 1.3e-250
Identity = 432/467 (92.51%), Postives = 454/467 (97.22%), Query Frame = 0

Query: 5   SIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 64
           S+IVPLP+NLL D A+P WMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA
Sbjct: 2   SLIVPLPSNLLPDPANPEWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 61

Query: 65  FMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFF 124
           FMALYAFACVLVCWVGWGYRLSFGDKL+PFWGKLNVALDQ+FLL+ AFSGK PSATMVFF
Sbjct: 62  FMALYAFACVLVCWVGWGYRLSFGDKLLPFWGKLNVALDQQFLLEQAFSGKLPSATMVFF 121

Query: 125 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYS 184
           QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFF+KMGLIDYS
Sbjct: 122 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS 181

Query: 185 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 244
           GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA
Sbjct: 182 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 241

Query: 245 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGW 304
           ANIDASLAVLNTHVCTATSLLTWLIFDI+FFRKPSVIGAVQGMITGLVCITPAAG+VQGW
Sbjct: 242 ANIDASLAVLNTHVCTATSLLTWLIFDIIFFRKPSVIGAVQGMITGLVCITPAAGVVQGW 301

Query: 305 AAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 364
           AAIIMG+LSGSIPWFTMMVVHKKS +LQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN
Sbjct: 302 AAIIMGLLSGSIPWFTMMVVHKKSAILQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 361

Query: 365 SIYYGAYKKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPL 424
            +YYGAY KYVGLFYGF++ QV LGFRQLGVQLLGI+FVTLIN+IVT++ICV LQYIVPL
Sbjct: 362 FVYYGAYNKYVGLFYGFNSKQVKLGFRQLGVQLLGILFVTLINIIVTSLICVLLQYIVPL 421

Query: 425 RMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMP--AKHRHA 470
           RM+EEDMAIGDEAAHGEEAYAIWGQGEK+D+SKYDIE+P  AKHRHA
Sbjct: 422 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPVVAKHRHA 468

BLAST of MS001289 vs. ExPASy TrEMBL
Match: A0A6J1K7F6 (Ammonium transporter OS=Cucurbita maxima OX=3661 GN=LOC111491842 PE=3 SV=1)

HSP 1 Score: 872.5 bits (2253), Expect = 8.2e-250
Identity = 431/469 (91.90%), Postives = 453/469 (96.59%), Query Frame = 0

Query: 5   SIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 64
           S+IVPLP+NLL D A+P WMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA
Sbjct: 2   SLIVPLPSNLLPDPANPEWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 61

Query: 65  FMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFF 124
           FMALYAFACVLVCWVGWGYRLSFGD+L+PFWGKLNVALDQ+FLL+ AFSGK PSATMVFF
Sbjct: 62  FMALYAFACVLVCWVGWGYRLSFGDRLLPFWGKLNVALDQQFLLEQAFSGKLPSATMVFF 121

Query: 125 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYS 184
           QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFF+KMGLIDYS
Sbjct: 122 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS 181

Query: 185 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 244
           GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA
Sbjct: 182 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 241

Query: 245 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGW 304
           ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAG+VQGW
Sbjct: 242 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVVQGW 301

Query: 305 AAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 364
           AAIIMG+LSGSIPWFTMMVVHKKS +LQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN
Sbjct: 302 AAIIMGLLSGSIPWFTMMVVHKKSAILQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 361

Query: 365 SIYYGAYKKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPL 424
            +YYGAY KYVGLFYGF++ QV LGFRQLGVQLLGI+FVTLIN+IVT++ICV LQYIVPL
Sbjct: 362 FVYYGAYNKYVGLFYGFNSKQVKLGFRQLGVQLLGILFVTLINIIVTSLICVLLQYIVPL 421

Query: 425 RMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMP--AKHRHAAE 472
           RM+EEDMAIGDEAAHGEEAYAIWGQGEK+D SKYDIE+P  AKHRH  +
Sbjct: 422 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDTSKYDIEVPAVAKHRHIGQ 470

BLAST of MS001289 vs. ExPASy TrEMBL
Match: A0A1S3BCV9 (Ammonium transporter OS=Cucumis melo OX=3656 GN=LOC103488277 PE=3 SV=1)

HSP 1 Score: 862.8 bits (2228), Expect = 6.5e-247
Identity = 428/473 (90.49%), Postives = 451/473 (95.35%), Query Frame = 0

Query: 1   MSNYSIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWA 60
           MSN  IIVPLPAN++ D  DPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWA
Sbjct: 1   MSN--IIVPLPANVVPDIGDPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWA 60

Query: 61  INSAFMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSAT 120
           INSAFMALYAFACVLVCWVGWGYRLSFG+KLVPFWGKLNVALDQ++LL   F+GKFP+AT
Sbjct: 61  INSAFMALYAFACVLVCWVGWGYRLSFGEKLVPFWGKLNVALDQEYLLQQGFAGKFPAAT 120

Query: 121 MVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGL 180
           MVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSP GFF KMGL
Sbjct: 121 MVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPEGFFAKMGL 180

Query: 181 IDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGG 240
           IDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGG
Sbjct: 181 IDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGG 240

Query: 241 DPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGL 300
           DPYAANIDASLAVLNTHVCTATSLLTWLIFDI+FFRKPSVIGAVQGMITGLVCITPAAG+
Sbjct: 241 DPYAANIDASLAVLNTHVCTATSLLTWLIFDIIFFRKPSVIGAVQGMITGLVCITPAAGV 300

Query: 301 VQGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAE 360
           VQGWAAIIMG+LSGSIPWFTMMVVHKKS +LQKVDDTMAV HTHAIAGSLGGILSGIFAE
Sbjct: 301 VQGWAAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAE 360

Query: 361 PRLNSIYYGAYKKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQY 420
           P LN+I+YG Y +Y+GLFYGF+T QV LGFRQLGVQLLGIIFVTL+NVIVT++ICV LQY
Sbjct: 361 PHLNNIFYGNYNQYIGLFYGFNTKQVRLGFRQLGVQLLGIIFVTLLNVIVTSLICVLLQY 420

Query: 421 IVPLRMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMP---AKHRHAA 471
           IVPLRM+EEDMAIGDEAAHGEEAYAIWGQGEK+D+SKYD+E P   AKHRHAA
Sbjct: 421 IVPLRMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDVEAPIAAAKHRHAA 471

BLAST of MS001289 vs. ExPASy TrEMBL
Match: A0A6P5TNZ2 (Ammonium transporter OS=Prunus avium OX=42229 GN=LOC110769126 PE=3 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 2.6e-219
Identity = 377/475 (79.37%), Postives = 425/475 (89.47%), Query Frame = 0

Query: 1   MSNYSIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWA 60
           MSN S   PLP NLL D A P WM+K DNAWQ+TAATLVGLQSVPGLIILYGGAVKKKWA
Sbjct: 1   MSN-STYPPLPINLLPDPASPDWMNKADNAWQLTAATLVGLQSVPGLIILYGGAVKKKWA 60

Query: 61  INSAFMALYAFACVLVCWVGWGYRLSFGDKL---VPFWGKLNVALDQKFLLDPAFSGKFP 120
           +NSAFMALYAFACVLVCWVGWGY +SFGD L   VPFWG+ +VAL+QK+LL+ AFSGKFP
Sbjct: 61  VNSAFMALYAFACVLVCWVGWGYHMSFGDPLIKGVPFWGRADVALNQKYLLEQAFSGKFP 120

Query: 121 SATMVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYK 180
           +ATMV+FQFVFAAITL+LIAGALLGRMNFYAWMLFVPLWLTFSYT  AFSIWSPNGF +K
Sbjct: 121 NATMVYFQFVFAAITLILIAGALLGRMNFYAWMLFVPLWLTFSYTIGAFSIWSPNGFLFK 180

Query: 181 MGLIDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGF 240
            GLIDYSGG+VIHLSSGVAGFTAAYWVGPR +KDRE FPPNNILLML GAGLLWMGWTGF
Sbjct: 181 KGLIDYSGGFVIHLSSGVAGFTAAYWVGPRSNKDRERFPPNNILLMLTGAGLLWMGWTGF 240

Query: 241 NGGDPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPA 300
           NGGDPY  ++DASLAVLNTHVCTATSLLTWL+ D++FFRKPSVIGAVQGMITGLVCITPA
Sbjct: 241 NGGDPYVVSVDASLAVLNTHVCTATSLLTWLMLDLIFFRKPSVIGAVQGMITGLVCITPA 300

Query: 301 AGLVQGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGI 360
           AG+VQGWAAI+MG+ SGSIPWFTMMVVHKKS +LQKVDDTMAV HTHAIAG+LGGIL+G+
Sbjct: 301 AGVVQGWAAILMGLCSGSIPWFTMMVVHKKSLLLQKVDDTMAVLHTHAIAGALGGILTGL 360

Query: 361 FAEPRLNSIYYGAYKKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVA 420
           FA PRL  ++  +Y++YVGLFYGF    V  GFRQLG+QLLGI ++ ++NV+VT+++C+ 
Sbjct: 361 FAHPRLCFLFTTSYEQYVGLFYGFQMGVVHTGFRQLGIQLLGIFYIVILNVVVTSLLCLL 420

Query: 421 LQYIVPLRMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSK--YDIEMPAKHRHAA 471
           +Q+IVPLRM++EDM IGDEAAHGEEAYAIWGQGEKL+NS+  YDIE  AK RHAA
Sbjct: 421 IQFIVPLRMSDEDMEIGDEAAHGEEAYAIWGQGEKLENSRYGYDIESSAKSRHAA 474

BLAST of MS001289 vs. TAIR 10
Match: AT2G38290.1 (ammonium transporter 2 )

HSP 1 Score: 555.8 bits (1431), Expect = 3.3e-158
Identity = 278/445 (62.47%), Postives = 345/445 (77.53%), Query Frame = 0

Query: 21  PGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALYAFACVLVCWVG 80
           P W++KGDNAWQ+TAATLVGLQS+PGL+ILY   VKKKWA+NSAFMALYAFA VL+CWV 
Sbjct: 13  PEWLNKGDNAWQLTAATLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLLCWVL 72

Query: 81  WGYRLSFGDKLVPFWGKLNVALDQKFLLDPA-------FSGKFPSATMVFFQFVFAAITL 140
             Y+++FG++L+PFWGK   A DQ +L   A        +  FP AT+V+FQF FAAIT 
Sbjct: 73  LCYKMAFGEELLPFWGKGGPAFDQGYLKGQAKIPNSNVAAPYFPMATLVYFQFTFAAITT 132

Query: 141 VLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGLIDYSGGYVIHLSS 200
           +L+AG++LGRMN  AWM FVPLWL FSYT  A+SIW   GF Y+ G+IDYSGGYVIHLSS
Sbjct: 133 ILVAGSVLGRMNIKAWMAFVPLWLIFSYTVGAYSIWG-GGFLYQWGVIDYSGGYVIHLSS 192

Query: 201 GVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYAANIDASLAV 260
           GVAGF AAYWVGPR   DRE FPPNN+LLMLAGAGLLWMGW+GFNGG PYAAN+ +S+AV
Sbjct: 193 GVAGFVAAYWVGPRPKADRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPYAANLTSSIAV 252

Query: 261 LNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLVQGWAAIIMGMLS 320
           LNT++  ATSLL W   D++FF KPSVIGA+QGM+TGL  +TP AGL+Q WAAII+G++S
Sbjct: 253 LNTNLSAATSLLVWTTLDVIFFGKPSVIGAIQGMVTGLAGVTPGAGLIQTWAAIIIGVVS 312

Query: 321 GSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLNSIYYGAYKK 380
           G+ PW +MM++HKKS +LQKVDDT+AVF+THA+AG LGGI++G+FA P L  +       
Sbjct: 313 GTAPWASMMIIHKKSALLQKVDDTLAVFYTHAVAGLLGGIMTGLFAHPDL-CVLVLPLPA 372

Query: 381 YVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQYIVPLRMTEEDMAI 440
             G FYG      G G +QL  QL G  F+ + NV+ TTII +A++  +PLRM EE++ I
Sbjct: 373 TRGAFYG------GNGGKQLLKQLAGAAFIAVWNVVSTTIILLAIRVFIPLRMAEEELGI 432

Query: 441 GDEAAHGEEAYAIWGQGEKLDNSKY 459
           GD+AAHGEEAYA+WG GEK D +++
Sbjct: 433 GDDAAHGEEAYALWGDGEKFDATRH 449

BLAST of MS001289 vs. TAIR 10
Match: AT2G38290.2 (ammonium transporter 2 )

HSP 1 Score: 429.1 bits (1102), Expect = 4.6e-120
Identity = 215/340 (63.24%), Postives = 267/340 (78.53%), Query Frame = 0

Query: 119 ATMVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKM 178
           AT+V+FQF FAAIT +L+AG++LGRMN  AWM FVPLWL FSYT  A+SIW   GF Y+ 
Sbjct: 2   ATLVYFQFTFAAITTILVAGSVLGRMNIKAWMAFVPLWLIFSYTVGAYSIWG-GGFLYQW 61

Query: 179 GLIDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFN 238
           G+IDYSGGYVIHLSSGVAGF AAYWVGPR   DRE FPPNN+LLMLAGAGLLWMGW+GFN
Sbjct: 62  GVIDYSGGYVIHLSSGVAGFVAAYWVGPRPKADRERFPPNNVLLMLAGAGLLWMGWSGFN 121

Query: 239 GGDPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAA 298
           GG PYAAN+ +S+AVLNT++  ATSLL W   D++FF KPSVIGA+QGM+TGL  +TP A
Sbjct: 122 GGAPYAANLTSSIAVLNTNLSAATSLLVWTTLDVIFFGKPSVIGAIQGMVTGLAGVTPGA 181

Query: 299 GLVQGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIF 358
           GL+Q WAAII+G++SG+ PW +MM++HKKS +LQKVDDT+AVF+THA+AG LGGI++G+F
Sbjct: 182 GLIQTWAAIIIGVVSGTAPWASMMIIHKKSALLQKVDDTLAVFYTHAVAGLLGGIMTGLF 241

Query: 359 AEPRLNSIYYGAYKKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVAL 418
           A P L  +         G FYG      G G +QL  QL G  F+ + NV+ TTII +A+
Sbjct: 242 AHPDL-CVLVLPLPATRGAFYG------GNGGKQLLKQLAGAAFIAVWNVVSTTIILLAI 301

Query: 419 QYIVPLRMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKY 459
           +  +PLRM EE++ IGD+AAHGEEAYA+WG GEK D +++
Sbjct: 302 RVFIPLRMAEEELGIGDDAAHGEEAYALWGDGEKFDATRH 333

BLAST of MS001289 vs. TAIR 10
Match: AT3G24290.1 (ammonium transporter 1;5 )

HSP 1 Score: 112.1 bits (279), Expect = 1.2e-24
Identity = 121/446 (27.13%), Postives = 202/446 (45.29%), Query Frame = 0

Query: 28  DNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALYAFACVLVCWVGWGYRLSF 87
           DN + + +A LV    + G  +L  G+V+ K  +N     +   A   + +  +GY  +F
Sbjct: 46  DNTYLLFSAYLVFAMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAF 105

Query: 88  GDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFFQFVFAAITLVLIAGALLGRMNFY 147
           G+    F G+ N  L Q F   P  +  +   +   +Q+ FA     + +G++  R  F 
Sbjct: 106 GESSDGFIGRHNFGL-QNF---PTLTSDY---SFFLYQWAFAIAAAGITSGSIAERTKFV 165

Query: 148 AWMLFVPLWLTFSYTFVAFSIWSPNGF---------FYKMGLIDYSGGYVIHLSSGVAGF 207
           A++++      F Y  V+   WSP+G+          +  G ID++G  V+H+  G+AG 
Sbjct: 166 AYLIYSSFLTGFVYPVVSHWFWSPDGWASPFRSEDRLFGTGAIDFAGSGVVHMVGGIAGL 225

Query: 208 TAAYWVGPRLSKDRE-----SFPPNNILLMLAGAGLLWMGWTGFNGGD------PY--AA 267
             A   GPR+ +  +     +   ++  L++ G  LLW GW GFN G       PY   +
Sbjct: 226 WGALIEGPRIGRFPDGGHAIALRGHSASLVVLGTFLLWFGWYGFNPGSFTKILIPYNSGS 285

Query: 268 NIDASLAVLNTHVCT----ATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGLV 327
           N      +  T V T     T+ LT L    +     +V     G++ G   IT    +V
Sbjct: 286 NYGQWSGIGRTAVTTTLSGCTAALTTLFGKRLLSGHWNVTDVCNGLLGGFAAITAGCSVV 345

Query: 328 QGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFA-E 387
             WAAI+ G ++  +     ++   K   L K DD +     H   G+ G I  G+FA E
Sbjct: 346 DPWAAIVCGFVASLV-----LIGCNKLAELLKYDDPLEAAQLHGGCGAWGLIFVGLFAKE 405

Query: 388 PRLNSIYYGAYKKYVGLFYGFSTAQVGLGFRQLGVQLLGII-FVTLINVIVTTIICVALQ 445
             +N +Y  +  ++ GLF       +G G + LG QL+ II  V  ++  + T+  + L+
Sbjct: 406 KYINEVYGASPGRHYGLF-------MGGGGKLLGAQLVQIIVIVGWVSATMGTLFFI-LK 465

BLAST of MS001289 vs. TAIR 10
Match: AT3G24300.1 (ammonium transporter 1;3 )

HSP 1 Score: 109.4 bits (272), Expect = 8.1e-24
Identity = 118/451 (26.16%), Postives = 197/451 (43.68%), Query Frame = 0

Query: 28  DNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALYAFACVLVCWVGWGYRLSF 87
           DN + + +A LV    + G  +L  G+V+ K  +N     +   A   + +  +GY  +F
Sbjct: 46  DNTYLLFSAYLVFAMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAF 105

Query: 88  GDKLVPFWGKLNVALDQKFLLDPAFSGKFPSAT----MVFFQFVFAAITLVLIAGALLGR 147
           G     F G+ N AL             FP+ T       +Q+ FA     + +G++  R
Sbjct: 106 GGSSEGFIGRHNFALRD-----------FPTPTADYSFFLYQWAFAIAAAGITSGSIAER 165

Query: 148 MNFYAWMLFVPLWLTFSYTFVAFSIWSPNGF----------FYKMGLIDYSGGYVIHLSS 207
             F A++++      F Y  V+   WSP+G+           +  G ID++G  V+H+  
Sbjct: 166 TQFVAYLIYSSFLTGFVYPVVSHWFWSPDGWASPFRSADDRLFSTGAIDFAGSGVVHMVG 225

Query: 208 GVAGFTAAYWVGPRLSKDRE-----SFPPNNILLMLAGAGLLWMGWTGFNGGD------P 267
           G+AG   A   GPR  +  +     +   ++  L++ G  LLW GW GFN G       P
Sbjct: 226 GIAGLWGALIEGPRRGRFEKGGRAIALRGHSASLVVLGTFLLWFGWYGFNPGSFTKILVP 285

Query: 268 YAANID------ASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITP 327
           Y +  +           +NT +   T+ LT L    +     +V     G++ G   IT 
Sbjct: 286 YNSGSNYGQWSGIGRTAVNTTLSGCTAALTTLFGKRLLSGHWNVTDVCNGLLGGFAAITA 345

Query: 328 AAGLVQGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSG 387
              +V+ WAAI+ G ++       +++   K   L + DD +     H   G+ G I  G
Sbjct: 346 GCSVVEPWAAIVCGFMAS-----VVLIGCNKLAELVQYDDPLEAAQLHGGCGAWGLIFVG 405

Query: 388 IFA-EPRLNSIYYGAYKKYVGLFYGFSTAQVGLGFRQLGVQLLGI-IFVTLINVIVTTII 445
           +FA E  LN +Y     +  GLF       +G G + LG QL+ I + V  ++  + T+ 
Sbjct: 406 LFAKEKYLNEVYGATPGRPYGLF-------MGGGGKLLGAQLVQILVIVGWVSATMGTLF 465

BLAST of MS001289 vs. TAIR 10
Match: AT4G13510.1 (ammonium transporter 1;1 )

HSP 1 Score: 102.1 bits (253), Expect = 1.3e-21
Identity = 120/454 (26.43%), Postives = 197/454 (43.39%), Query Frame = 0

Query: 28  DNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALYAFACVLVCWVGWGYRLSF 87
           DN + + +A LV    + G  +L  G+V+ K  +N     +   A   + +  +GY  +F
Sbjct: 42  DNTYLLFSAYLVFSMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAF 101

Query: 88  GDKLVPFWGKLNVALDQKFLLDPAFSGKFPSATMVFFQFVFAAITLVLIAGALLGRMNFY 147
           G     F GK    L       P  S  + +     +Q+ FA     + +G++  R  F 
Sbjct: 102 GSPSNGFIGKHYFGLKD----IPTASADYSN---FLYQWAFAIAAAGITSGSIAERTQFV 161

Query: 148 AWMLFVPLWLTFSYTFVAFSIWSPNGF----------FYKMGLIDYSGGYVIHLSSGVAG 207
           A++++      F Y  V+   WS +G+           +  G ID++G  V+H+  G+AG
Sbjct: 162 AYLIYSSFLTGFVYPVVSHWFWSVDGWASPFRTDGDLLFSTGAIDFAGSGVVHMVGGIAG 221

Query: 208 FTAAYWVGPRLSKDRE-----SFPPNNILLMLAGAGLLWMGWTGFNGGD----------- 267
              A   GPRL +        +   ++  L++ G  LLW GW GFN G            
Sbjct: 222 LWGALIEGPRLGRFDNGGRAIALRGHSASLVVLGTFLLWFGWYGFNPGSFNKILVTYETG 281

Query: 268 PYAANIDA-SLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGL 327
            Y     A     + T +   T+ LT L    +     +V     G++ G   IT    +
Sbjct: 282 TYNGQWSAVGRTAVTTTLAGCTAALTTLFGKRLLSGHWNVTDVCNGLLGGFAAITGGCSV 341

Query: 328 VQGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAE 387
           V+ WAAII G ++  +    ++  +K +  L K DD +     H   G+ G I + +FA+
Sbjct: 342 VEPWAAIICGFVAALV----LLGCNKLAEKL-KYDDPLEAAQLHGGCGAWGLIFTALFAQ 401

Query: 388 PR-LNSIYYGAYKKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVT-LINVIVTTIICVAL 447
            + LN IY     +  GLF       +G G + LG QL+ II +T  ++  + T+  + L
Sbjct: 402 EKYLNQIYGNKPGRPHGLF-------MGGGGKLLGAQLIQIIVITGWVSATMGTLFFI-L 461

Query: 448 QYIVPLRMTEED-MAIGDEAAHGEEAYAIWGQGE 452
           + +  LR++ ED MA  D   HG  AY  +   E
Sbjct: 462 KKMKLLRISSEDEMAGMDMTRHGGFAYMYFDDDE 474

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022131630.11.7e-27099.79ammonium transporter 3 member 1 [Momordica charantia][more]
XP_022962592.12.6e-25092.51ammonium transporter 3 member 1-like [Cucurbita moschata] >KAG6598591.1 Ammonium... [more]
XP_023546976.14.4e-25092.51ammonium transporter 3 member 1-like [Cucurbita pepo subsp. pepo][more]
XP_022996665.11.7e-24991.90ammonium transporter 3 member 1-like [Cucurbita maxima][more]
XP_004138867.17.1e-24891.10ammonium transporter 3 member 1 [Cucumis sativus] >KAE8652192.1 hypothetical pro... [more]
Match NameE-valueIdentityDescription
A0A072VHJ18.0e-21077.06Ammonium transporter 2 member 5 OS=Medicago truncatula OX=3880 GN=AMT2-5 PE=2 SV... [more]
Q84KJ66.2e-17064.36Ammonium transporter 3 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3... [more]
G7L1W71.8e-16964.24Ammonium transporter 2 member 4 OS=Medicago truncatula OX=3880 GN=AMT2-4 PE=2 SV... [more]
Q69T293.7e-16762.75Ammonium transporter 3 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3... [more]
G7LAA84.9e-16764.94Ammonium transporter 2 member 3 OS=Medicago truncatula OX=3880 GN=AMT2-3 PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1BQ838.4e-27199.79Ammonium transporter OS=Momordica charantia OX=3673 GN=LOC111004759 PE=3 SV=1[more]
A0A6J1HD341.3e-25092.51Ammonium transporter OS=Cucurbita moschata OX=3662 GN=LOC111462995 PE=3 SV=1[more]
A0A6J1K7F68.2e-25091.90Ammonium transporter OS=Cucurbita maxima OX=3661 GN=LOC111491842 PE=3 SV=1[more]
A0A1S3BCV96.5e-24790.49Ammonium transporter OS=Cucumis melo OX=3656 GN=LOC103488277 PE=3 SV=1[more]
A0A6P5TNZ22.6e-21979.37Ammonium transporter OS=Prunus avium OX=42229 GN=LOC110769126 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G38290.13.3e-15862.47ammonium transporter 2 [more]
AT2G38290.24.6e-12063.24ammonium transporter 2 [more]
AT3G24290.11.2e-2427.13ammonium transporter 1;5 [more]
AT3G24300.18.1e-2426.16ammonium transporter 1;3 [more]
AT4G13510.11.3e-2126.43ammonium transporter 1;1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002229Blood group Rhesus C/E/D polypeptidePRINTSPR00342RHESUSRHDcoord: 125..141
score: 25.49
coord: 221..239
score: 41.52
coord: 252..274
score: 27.54
IPR029020Ammonium/urea transporterGENE3D1.10.3430.10Ammonium transporter AmtB like domainscoord: 13..448
e-value: 4.0E-124
score: 416.7
IPR024041Ammonium transporter AmtB-like domainPFAMPF00909Ammonium_transpcoord: 30..444
e-value: 1.0E-83
score: 281.3
IPR001905Ammonium transporterTIGRFAMTIGR00836TIGR00836coord: 28..444
e-value: 1.3E-113
score: 378.0
NoneNo IPR availablePANTHERPTHR43029:SF7AMMONIUM TRANSPORTERcoord: 8..466
NoneNo IPR availablePANTHERPTHR43029AMMONIUM TRANSPORTER MEP2coord: 8..466
NoneNo IPR availableSUPERFAMILY111352Ammonium transportercoord: 23..426
IPR018047Ammonium transporter, conserved sitePROSITEPS01219AMMONIUM_TRANSPcoord: 182..207

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS001289.1MS001289.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0072488 ammonium transmembrane transport
biological_process GO:0015696 ammonium transport
cellular_component GO:0005887 integral component of plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0008519 ammonium transmembrane transporter activity