MS001278 (gene) Bitter gourd (TR) v1

BLAST of MS001278 vs. NCBI nr
Match: XP_022131978.1 (gibberellin 3-beta-dioxygenase 1-like isoform X2 [Momordica charantia])

HSP 1 Score: 705.3 bits (1819), Expect = 2.6e-199
Identity = 341/343 (99.42%), Postives = 343/343 (100.00%), Query Frame = 0

Query: 1   MADQELPPPSHVVPLDFDSVETVPESHSWPDSNGLSSTDEKVTIPLIDLVDDNASELIGR 60
           MADQELPPPSHVVPLDFDSVETVPESHSWPDSNGLSSTDEKVTIPLIDLVDDNASELIGR
Sbjct: 1   MADQELPPPSHVVPLDFDSVETVPESHSWPDSNGLSSTDEKVTIPLIDLVDDNASELIGR 60

Query: 61  ACEQWGMFQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVISKYY 120
           ACEQWGMFQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVISKYY
Sbjct: 61  ACEQWGMFQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVISKYY 120

Query: 121 QKYMWHEGFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLDISE 180
           QKYMWHEGFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLDISE
Sbjct: 121 QKYMWHEGFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLDISE 180

Query: 181 QVIEKLSPSNNDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQVF 240
           QVIEKLSPS+NDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQVF
Sbjct: 181 QVIEKLSPSSNDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQVF 240

Query: 241 KDEVGWIPLSPMSGTLVVNVGDLLHILSNGRFRSILHRVVIQGKEHRLSMAYFYYPPNEL 300
           KDEVGWIPLSPMSGTLVVN+GDLLHILSNGRFRSILHRVVIQGKEHRLSMAYFYYPPNEL
Sbjct: 241 KDEVGWIPLSPMSGTLVVNIGDLLHILSNGRFRSILHRVVIQGKEHRLSMAYFYYPPNEL 300

Query: 301 YISPHCNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIKI 344
           YISPHCNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIKI
Sbjct: 301 YISPHCNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIKI 343

BLAST of MS001278 vs. NCBI nr
Match: XP_022131977.1 (gibberellin 3-beta-dioxygenase 1-like isoform X1 [Momordica charantia])

HSP 1 Score: 705.3 bits (1819), Expect = 2.6e-199
Identity = 341/343 (99.42%), Postives = 343/343 (100.00%), Query Frame = 0

Query: 1   MADQELPPPSHVVPLDFDSVETVPESHSWPDSNGLSSTDEKVTIPLIDLVDDNASELIGR 60
           MADQELPPPSHVVPLDFDSVETVPESHSWPDSNGLSSTDEKVTIPLIDLVDDNASELIGR
Sbjct: 7   MADQELPPPSHVVPLDFDSVETVPESHSWPDSNGLSSTDEKVTIPLIDLVDDNASELIGR 66

Query: 61  ACEQWGMFQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVISKYY 120
           ACEQWGMFQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVISKYY
Sbjct: 67  ACEQWGMFQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVISKYY 126

Query: 121 QKYMWHEGFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLDISE 180
           QKYMWHEGFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLDISE
Sbjct: 127 QKYMWHEGFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLDISE 186

Query: 181 QVIEKLSPSNNDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQVF 240
           QVIEKLSPS+NDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQVF
Sbjct: 187 QVIEKLSPSSNDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQVF 246

Query: 241 KDEVGWIPLSPMSGTLVVNVGDLLHILSNGRFRSILHRVVIQGKEHRLSMAYFYYPPNEL 300
           KDEVGWIPLSPMSGTLVVN+GDLLHILSNGRFRSILHRVVIQGKEHRLSMAYFYYPPNEL
Sbjct: 247 KDEVGWIPLSPMSGTLVVNIGDLLHILSNGRFRSILHRVVIQGKEHRLSMAYFYYPPNEL 306

Query: 301 YISPHCNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIKI 344
           YISPHCNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIKI
Sbjct: 307 YISPHCNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIKI 349

BLAST of MS001278 vs. NCBI nr
Match: XP_022951873.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita moschata])

HSP 1 Score: 527.7 bits (1358), Expect = 7.4e-146
Identity = 253/347 (72.91%), Postives = 292/347 (84.15%), Query Frame = 0

Query: 1   MADQELPPPSHVVPLDFDSVETVPESHSWPDSNGLSST---DEKVTIPLIDLVDDNASEL 60
           MADQE       +PLDF+SV+TVPESH WPDS+ LSS    DEK+++P IDL+DD ASEL
Sbjct: 1   MADQEF------LPLDFNSVQTVPESHLWPDSDELSSVIDLDEKISLPSIDLLDDGASEL 60

Query: 61  IGRACEQWGMFQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVIS 120
           +GRACEQWGMFQLTNHG+ +TLIAE E+EARRLF LP ++KM  L S+D VTGYG AVIS
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAETEEEARRLFTLPVKEKMKTLRSADRVTGYGTAVIS 120

Query: 121 KYYQKYMWHEGFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLD 180
           KY+ K MWHEGFTILGSP DDAKKLWPS+ K FCDVMEEYQ +M+ L+D++I+L+FKFLD
Sbjct: 121 KYHPKCMWHEGFTILGSPADDAKKLWPSDSKRFCDVMEEYQSEMKRLSDRLIKLIFKFLD 180

Query: 181 ISEQVIEKLSPSNNDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGL 240
           IS +   KL  S +D G+ YTAL+LNSYPPCPDP QVMGLAAHTDTSLFTI+HQA  DGL
Sbjct: 181 ISVEETMKLLSSMDDSGKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGL 240

Query: 241 QVFKDEVGWIPLSPMSGTLVVNVGDLLHILSNGRFRSILHRVVIQGKE-HRLSMAYFYYP 300
           Q+FKD+ GW+PLSPM G+ VVNVGDLLHI+SNGRFRSILHRV IQGK+ HR S+AYFYYP
Sbjct: 241 QIFKDKAGWVPLSPMRGSFVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYP 300

Query: 301 PNELYISPHCNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIKI 344
           P  LYISPHC PLSESPQTPLYRCV V+EYF+MKAKN G GLST+KI
Sbjct: 301 PPYLYISPHCKPLSESPQTPLYRCVSVKEYFTMKAKNNGEGLSTVKI 341

BLAST of MS001278 vs. NCBI nr
Match: AAF80661.1 (putative gibberellin 3 beta hydroxylase [Citrullus lanatus subsp. vulgaris])

HSP 1 Score: 525.0 bits (1351), Expect = 4.8e-145
Identity = 253/349 (72.49%), Postives = 296/349 (84.81%), Query Frame = 0

Query: 1   MADQELPPPSHVVPLDFDSVETVPESHSWPDSNGLS---STDEKVTIPLIDLVDDNASEL 60
           MADQELPPPS +VPLDF+S++TVPESH WP+S  LS     DE++++P+ID +DD ASEL
Sbjct: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60

Query: 61  IGRACEQWGMFQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYS-SDSVTGYGMAVI 120
           +GRACEQWGMFQL NHGVP+TLI EAE+E RRLF LP RQKM  L + S + TGYGMA I
Sbjct: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGI 120

Query: 121 SKYYQKYMWHEGFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFL 180
           SKY+QK MWHEGFT+ GSPVDDAKKLWP++YK FCDVMEEYQ QM+ L ++IIRL+FKFL
Sbjct: 121 SKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFL 180

Query: 181 DISEQVIEKLSPSNND-VGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARID 240
           DISE+ + KL  S ++  G+ Y ALRLNSYPPCPDPS+VMGLAAHTDTSLFTI+HQAR D
Sbjct: 181 DISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHD 240

Query: 241 GLQVFKDEVGWIPLSPMSGTLVVNVGDLLHILSNGRFRSILHRVVI-QGKEHRLSMAYFY 300
           GLQVFKD  GW+PLSPMSGTL+VNVGD+LHILSNGRF SILHRV+I + KEHR S+AYFY
Sbjct: 241 GLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFY 300

Query: 301 YPPNELYISPHCNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIKI 344
            PP +LYISP+C PLSESPQ PLYRCV V+EYF++KAK  G+GLS IKI
Sbjct: 301 DPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 349

BLAST of MS001278 vs. NCBI nr
Match: XP_023537854.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 523.9 bits (1348), Expect = 1.1e-144
Identity = 252/347 (72.62%), Postives = 292/347 (84.15%), Query Frame = 0

Query: 1   MADQELPPPSHVVPLDFDSVETVPESHSWPDSNGLSST---DEKVTIPLIDLVDDNASEL 60
           MAD+E       +PLDF+SV+TVPESH WPDS+ LSS    DEK+++P IDL+DD ASEL
Sbjct: 1   MADKEF------LPLDFNSVQTVPESHLWPDSDELSSVIDLDEKISLPSIDLLDDGASEL 60

Query: 61  IGRACEQWGMFQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVIS 120
           +GRACEQWGMFQLTNHG+ +TLIA+ E+EARRLF LP ++KM  L S+D VTGYG AVIS
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAKMEEEARRLFTLPAKEKMKTLRSADRVTGYGTAVIS 120

Query: 121 KYYQKYMWHEGFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLD 180
           KY+ K MWHEGFTILGSP DDAKKLWPS+ K FCDVMEEYQ +M+ L+D++I+L+FKFLD
Sbjct: 121 KYHSKCMWHEGFTILGSPADDAKKLWPSDSKRFCDVMEEYQSEMKRLSDRLIKLIFKFLD 180

Query: 181 ISEQVIEKLSPSNNDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGL 240
           ISE+   KL  S +D G+ YTAL+LNSYPPCPDP QVMGLAAHTDTSLFTI+HQA  DGL
Sbjct: 181 ISEEETMKLLSSMDDSGKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGL 240

Query: 241 QVFKDEVGWIPLSPMSGTLVVNVGDLLHILSNGRFRSILHRVVIQGKE-HRLSMAYFYYP 300
           Q+FKD+ GWIPLSPM G+ VVNVGDLLHI+SNGRFRSILHRV IQGK+ HR S+AYFYYP
Sbjct: 241 QIFKDKAGWIPLSPMRGSFVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYP 300

Query: 301 PNELYISPHCNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIKI 344
           P  LYI+PHC  LSESPQTPLYRCV V+EYF+MKAKN G GLSTIKI
Sbjct: 301 PPYLYITPHCKALSESPQTPLYRCVSVKEYFTMKAKNNGEGLSTIKI 341

BLAST of MS001278 vs. ExPASy Swiss-Prot
Match: O24648 (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 1.0e-81
Identity = 150/336 (44.64%), Postives = 217/336 (64.58%), Query Frame = 0

Query: 16  DFDSVETVPESHSWP--------DSNGLSSTDEKVTIPLIDLVDDNASELIGRACEQWGM 75
           DF+S++ +PES++W         DSN +   +   T+P+IDL D NAS+LIG AC+ WG+
Sbjct: 21  DFNSLQELPESYNWTHLDDHTLIDSNNIMK-ESTTTVPVIDLNDPNASKLIGLACKTWGV 80

Query: 76  FQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVISKYYQKYMWHE 135
           +Q+ NHG+P +L+ + +   + LF LP+ QK  A  S D V+GYG+A IS ++ K MW+E
Sbjct: 81  YQVMNHGIPLSLLEDIQWLGQTLFSLPSHQKHKATRSPDGVSGYGIARISSFFPKLMWYE 140

Query: 136 GFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLDISEQVIEKLS 195
           GFTI+GSP+D  ++LWP +Y  FCD++ +Y   M+ LA  ++ L+   L I+++ I K +
Sbjct: 141 GFTIVGSPLDHFRELWPQDYTRFCDIVVQYDETMKKLAGTLMCLMLDSLGITKEDI-KWA 200

Query: 196 PSNNDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQVFKDEVGWI 255
            S     +   AL+LNSYP CPDP   MGLA HTD++  TIL Q  I GLQV ++  GWI
Sbjct: 201 GSKAQFEKACAALQLNSYPSCPDPDHAMGLAPHTDSTFLTILSQNDISGLQVNREGSGWI 260

Query: 256 PLSPMSGTLVVNVGDLLHILSNGRFRSILHRVVIQGKEHRLSMAYFYYPPNELYISPHCN 315
            + P+ G LVVNVGDL HILSNG + S+LHRV++     R S+AY Y PP+ + I PH  
Sbjct: 261 TVPPLQGGLVVNVGDLFHILSNGLYPSVLHRVLVNRTRQRFSVAYLYGPPSNVEICPHAK 320

Query: 316 PLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIKI 344
            +  + + PLYR V   EY   KAK+  + LS++++
Sbjct: 321 LIGPT-KPPLYRSVTWNEYLGTKAKHFNKALSSVRL 353

BLAST of MS001278 vs. ExPASy Swiss-Prot
Match: Q9ZT84 (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 295.8 bits (756), Expect = 6.2e-79
Identity = 146/338 (43.20%), Postives = 218/338 (64.50%), Query Frame = 0

Query: 9   PSHVVPLDFDSVETVPESHSWPDSNGL----SSTDEKVTIPLIDLVDDNASELIGRACEQ 68
           P H+   +    +++P+S++W   + L    S++DE  T+PLIDL D + + L+G AC  
Sbjct: 13  PIHIPLSNPPDFKSLPDSYTWTPKDDLLFSASASDE--TLPLIDLSDIHVATLVGHACTT 72

Query: 69  WGMFQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVISKYYQKYM 128
           WG FQ+TNHGVP  L+ + E     LF LP ++K+ A  S + V+GYG+A I+ ++ K M
Sbjct: 73  WGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYGVARIASFFNKKM 132

Query: 129 WHEGFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLDISEQVIE 188
           W EGFT++GSP+ D +KLWPS +  +C+++EEY+  M+ LA K++      L + E+ I+
Sbjct: 133 WSEGFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFALGSLGVEEKDIQ 192

Query: 189 KLSPSNNDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQVFKDEV 248
              P N+D       ++LN YP CP+P + MGLAAHTD++L TIL+Q    GLQVF+D+V
Sbjct: 193 WAGP-NSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDV 252

Query: 249 GWIPLSPMSGTLVVNVGDLLHILSNGRFRSILHRVVIQGKEHRLSMAYFYYPPNELYISP 308
           GW+   P+ G+LVVNVGDLLHIL+NG F S+LHR  +     R SMAY + PP+++ ISP
Sbjct: 253 GWVTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHVRSRFSMAYLWGPPSDIMISP 312

Query: 309 HCNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIK 343
               L +  Q+PLY  +  ++Y + KA +  + LS I+
Sbjct: 313 -LPKLVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

BLAST of MS001278 vs. ExPASy Swiss-Prot
Match: Q39103 (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 295.0 bits (754), Expect = 1.1e-78
Identity = 152/342 (44.44%), Postives = 217/342 (63.45%), Query Frame = 0

Query: 8   PPSHVVPLDFDSVETVPESHSWPDSNGL------SSTDEKVTIPLIDLVDDNASELIGRA 67
           P SH+   DF S+  +P+S+ W   + L      S       IPLIDL   +A+  IG A
Sbjct: 17  PHSHIP--DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDHPDATNQIGHA 76

Query: 68  CEQWGMFQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVISKYYQ 127
           C  WG FQ++NHGVP  L+ + E     LF LP ++K+ +  S   V+GYG+A I+ ++ 
Sbjct: 77  CRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSGYGVARIASFFN 136

Query: 128 KYMWHEGFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLDISEQ 187
           K MW EGFTI GSP++D +KLWP  + ++CD++EEY+  M+ LA K++ L    L +SE+
Sbjct: 137 KQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWLALNSLGVSEE 196

Query: 188 VIEKLSPSNNDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQVFK 247
            IE  S S +D+     AL+LN YP CP+P + MGLAAHTD++L TIL+Q    GLQVF+
Sbjct: 197 DIEWASLS-SDLNWAQAALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGLQVFR 256

Query: 248 DEVGWIPLSPMSGTLVVNVGDLLHILSNGRFRSILHRVVIQGKEHRLSMAYFYYPPNELY 307
           D++GW+ + P  G+LVVNVGDL HILSNG F+S+LHR  +     RLS+A+ + P +++ 
Sbjct: 257 DDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQTRARLSVAFLWGPQSDIK 316

Query: 308 ISPHCNPLSESP-QTPLYRCVKVREYFSMKAKNMGRGLSTIK 343
           ISP   P   SP ++PLY+ V  +EY   KA +  + LS I+
Sbjct: 317 ISP--VPKLVSPVESPLYQSVTWKEYLRTKATHFNKALSMIR 353

BLAST of MS001278 vs. ExPASy Swiss-Prot
Match: Q9SVS8 (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 5.3e-70
Identity = 137/337 (40.65%), Postives = 205/337 (60.83%), Query Frame = 0

Query: 12  VVPLDFDSVETVPESHSWPDSNGLSSTDEKVTIPLIDLVDDNASELIGRACEQWGMFQLT 71
           ++PLDF + +T+P+SH W      +++     IP+I L +     L+ +ACE+WG+F +T
Sbjct: 18  IIPLDFTNTKTLPDSHVWSKPEPETTSG---PIPVISLSNPEEHGLLRQACEEWGVFHIT 77

Query: 72  NHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVISKYYQKYMWHEGFTI 131
           +HGV  +L+   + + +RLF LP  +K++A+ S D  TGYG+  IS +Y K MW EGF++
Sbjct: 78  DHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDESTGYGVVRISMFYDKLMWSEGFSV 137

Query: 132 LGSPV-DDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLDISEQVIEKLSPSN 191
           +GS +   A  LWP ++  FC+VMEEYQ  M  L+ ++I ++   L ++ + +  L P  
Sbjct: 138 MGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISMLMGSLGLTHEDLGWLVPDK 197

Query: 192 NDVG--QLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQV-FKDEVG-- 251
              G   + + L+LNSYP CPDP   MGLA HTD+SL TIL+Q  I GL++    E G  
Sbjct: 198 TGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIESPQEEGSR 257

Query: 252 WIPLSPMSGTLVVNVGDLLHILSNGRFRSILHRVVIQGKEHRLSMAYFYYPPNELYISPH 311
           WI + P+ G+LVV +GDL HI+SNG+FRS +HR V+    HR+S AYF  PP  L I P 
Sbjct: 258 WIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVNKTHHRVSAAYFAGPPKNLQIGP- 317

Query: 312 CNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIK 343
               S+    P+YR +   EY + KA +  + L+  +
Sbjct: 318 --LTSDKNHPPIYRRLIWEEYLAAKATHFNKALTLFR 348

BLAST of MS001278 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 1.8e-65
Identity = 129/343 (37.61%), Postives = 208/343 (60.64%), Query Frame = 0

Query: 19  SVETVPESHSWP---------DSNGLSSTDEKVTIPLIDLVDDNASELIGRACEQWGMFQ 78
           S++T+PES +W           S+ +S    + +IP+IDL + + + LIG A + WG FQ
Sbjct: 15  SLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQ 74

Query: 79  LTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVISKYYQKYMWHEGF 138
           + NHG+ + L+ + E  ++ LFD+P+ +K+ A  S   V+GYG   IS +++K MW EGF
Sbjct: 75  IANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWSEGF 134

Query: 139 TILGSPVDDAKK-----LWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLDISEQVIE 198
           TI     DD+ +     LWP ++  +C +++EY  +M  LA +++  +   L ++ + IE
Sbjct: 135 TI----ADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIE 194

Query: 199 ---KLSPSNNDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQVFK 258
              KL  S + VG+   A+RLN YP CP+P + MGLAAHTD+++ TILHQ+   GLQVF+
Sbjct: 195 WAHKLEKSGSKVGR--GAIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFR 254

Query: 259 DEVGWIPLSPMSGTLVVNVGDLLHILSNGRFRSILHRVVIQGKEHRLSMAYFY-YPPNEL 318
           +E GW+ + P  G LVVN+GDL HILSNG+  S++HR  +     R+S+AY +  P  ++
Sbjct: 255 EESGWVTVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRSRISIAYLWGGPAGDV 314

Query: 319 YISPHCNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIKI 344
            I+P  + L+   +  LYR +  +EY  +K +   + +  I++
Sbjct: 315 QIAP-ISKLTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRV 350

BLAST of MS001278 vs. ExPASy TrEMBL
Match: A0A6J1BSK2 (gibberellin 3-beta-dioxygenase 1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004962 PE=3 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 1.3e-199
Identity = 341/343 (99.42%), Postives = 343/343 (100.00%), Query Frame = 0

Query: 1   MADQELPPPSHVVPLDFDSVETVPESHSWPDSNGLSSTDEKVTIPLIDLVDDNASELIGR 60
           MADQELPPPSHVVPLDFDSVETVPESHSWPDSNGLSSTDEKVTIPLIDLVDDNASELIGR
Sbjct: 7   MADQELPPPSHVVPLDFDSVETVPESHSWPDSNGLSSTDEKVTIPLIDLVDDNASELIGR 66

Query: 61  ACEQWGMFQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVISKYY 120
           ACEQWGMFQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVISKYY
Sbjct: 67  ACEQWGMFQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVISKYY 126

Query: 121 QKYMWHEGFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLDISE 180
           QKYMWHEGFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLDISE
Sbjct: 127 QKYMWHEGFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLDISE 186

Query: 181 QVIEKLSPSNNDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQVF 240
           QVIEKLSPS+NDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQVF
Sbjct: 187 QVIEKLSPSSNDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQVF 246

Query: 241 KDEVGWIPLSPMSGTLVVNVGDLLHILSNGRFRSILHRVVIQGKEHRLSMAYFYYPPNEL 300
           KDEVGWIPLSPMSGTLVVN+GDLLHILSNGRFRSILHRVVIQGKEHRLSMAYFYYPPNEL
Sbjct: 247 KDEVGWIPLSPMSGTLVVNIGDLLHILSNGRFRSILHRVVIQGKEHRLSMAYFYYPPNEL 306

Query: 301 YISPHCNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIKI 344
           YISPHCNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIKI
Sbjct: 307 YISPHCNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIKI 349

BLAST of MS001278 vs. ExPASy TrEMBL
Match: A0A6J1BUZ4 (gibberellin 3-beta-dioxygenase 1-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111004962 PE=3 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 1.3e-199
Identity = 341/343 (99.42%), Postives = 343/343 (100.00%), Query Frame = 0

Query: 1   MADQELPPPSHVVPLDFDSVETVPESHSWPDSNGLSSTDEKVTIPLIDLVDDNASELIGR 60
           MADQELPPPSHVVPLDFDSVETVPESHSWPDSNGLSSTDEKVTIPLIDLVDDNASELIGR
Sbjct: 1   MADQELPPPSHVVPLDFDSVETVPESHSWPDSNGLSSTDEKVTIPLIDLVDDNASELIGR 60

Query: 61  ACEQWGMFQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVISKYY 120
           ACEQWGMFQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVISKYY
Sbjct: 61  ACEQWGMFQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVISKYY 120

Query: 121 QKYMWHEGFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLDISE 180
           QKYMWHEGFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLDISE
Sbjct: 121 QKYMWHEGFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLDISE 180

Query: 181 QVIEKLSPSNNDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQVF 240
           QVIEKLSPS+NDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQVF
Sbjct: 181 QVIEKLSPSSNDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQVF 240

Query: 241 KDEVGWIPLSPMSGTLVVNVGDLLHILSNGRFRSILHRVVIQGKEHRLSMAYFYYPPNEL 300
           KDEVGWIPLSPMSGTLVVN+GDLLHILSNGRFRSILHRVVIQGKEHRLSMAYFYYPPNEL
Sbjct: 241 KDEVGWIPLSPMSGTLVVNIGDLLHILSNGRFRSILHRVVIQGKEHRLSMAYFYYPPNEL 300

Query: 301 YISPHCNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIKI 344
           YISPHCNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIKI
Sbjct: 301 YISPHCNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIKI 343

BLAST of MS001278 vs. ExPASy TrEMBL
Match: A0A6J1GIY0 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111454608 PE=3 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 3.6e-146
Identity = 253/347 (72.91%), Postives = 292/347 (84.15%), Query Frame = 0

Query: 1   MADQELPPPSHVVPLDFDSVETVPESHSWPDSNGLSST---DEKVTIPLIDLVDDNASEL 60
           MADQE       +PLDF+SV+TVPESH WPDS+ LSS    DEK+++P IDL+DD ASEL
Sbjct: 1   MADQEF------LPLDFNSVQTVPESHLWPDSDELSSVIDLDEKISLPSIDLLDDGASEL 60

Query: 61  IGRACEQWGMFQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVIS 120
           +GRACEQWGMFQLTNHG+ +TLIAE E+EARRLF LP ++KM  L S+D VTGYG AVIS
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAETEEEARRLFTLPVKEKMKTLRSADRVTGYGTAVIS 120

Query: 121 KYYQKYMWHEGFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLD 180
           KY+ K MWHEGFTILGSP DDAKKLWPS+ K FCDVMEEYQ +M+ L+D++I+L+FKFLD
Sbjct: 121 KYHPKCMWHEGFTILGSPADDAKKLWPSDSKRFCDVMEEYQSEMKRLSDRLIKLIFKFLD 180

Query: 181 ISEQVIEKLSPSNNDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGL 240
           IS +   KL  S +D G+ YTAL+LNSYPPCPDP QVMGLAAHTDTSLFTI+HQA  DGL
Sbjct: 181 ISVEETMKLLSSMDDSGKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQACNDGL 240

Query: 241 QVFKDEVGWIPLSPMSGTLVVNVGDLLHILSNGRFRSILHRVVIQGKE-HRLSMAYFYYP 300
           Q+FKD+ GW+PLSPM G+ VVNVGDLLHI+SNGRFRSILHRV IQGK+ HR S+AYFYYP
Sbjct: 241 QIFKDKAGWVPLSPMRGSFVVNVGDLLHIMSNGRFRSILHRVTIQGKKVHRFSLAYFYYP 300

Query: 301 PNELYISPHCNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIKI 344
           P  LYISPHC PLSESPQTPLYRCV V+EYF+MKAKN G GLST+KI
Sbjct: 301 PPYLYISPHCKPLSESPQTPLYRCVSVKEYFTMKAKNNGEGLSTVKI 341

BLAST of MS001278 vs. ExPASy TrEMBL
Match: Q9LM06 (Putative gibberellin 3 beta hydroxylase OS=Citrullus lanatus OX=3654 GN=WGA3H PE=2 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 2.3e-145
Identity = 253/349 (72.49%), Postives = 296/349 (84.81%), Query Frame = 0

Query: 1   MADQELPPPSHVVPLDFDSVETVPESHSWPDSNGLS---STDEKVTIPLIDLVDDNASEL 60
           MADQELPPPS +VPLDF+S++TVPESH WP+S  LS     DE++++P+ID +DD ASEL
Sbjct: 1   MADQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASEL 60

Query: 61  IGRACEQWGMFQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYS-SDSVTGYGMAVI 120
           +GRACEQWGMFQL NHGVP+TLI EAE+E RRLF LP RQKM  L + S + TGYGMA I
Sbjct: 61  LGRACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGI 120

Query: 121 SKYYQKYMWHEGFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFL 180
           SKY+QK MWHEGFT+ GSPVDDAKKLWP++YK FCDVMEEYQ QM+ L ++IIRL+FKFL
Sbjct: 121 SKYHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRFCDVMEEYQSQMKGLGERIIRLIFKFL 180

Query: 181 DISEQVIEKLSPSNND-VGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARID 240
           DISE+ + KL  S ++  G+ Y ALRLNSYPPCPDPS+VMGLAAHTDTSLFTI+HQAR D
Sbjct: 181 DISEEEMMKLLTSTDESAGKPYMALRLNSYPPCPDPSRVMGLAAHTDTSLFTIVHQARHD 240

Query: 241 GLQVFKDEVGWIPLSPMSGTLVVNVGDLLHILSNGRFRSILHRVVI-QGKEHRLSMAYFY 300
           GLQVFKD  GW+PLSPMSGTL+VNVGD+LHILSNGRF SILHRV+I + KEHR S+AYFY
Sbjct: 241 GLQVFKDGTGWVPLSPMSGTLIVNVGDILHILSNGRFPSILHRVMIHENKEHRFSLAYFY 300

Query: 301 YPPNELYISPHCNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIKI 344
            PP +LYISP+C PLSESPQ PLYRCV V+EYF++KAK  G+GLS IKI
Sbjct: 301 DPPGDLYISPYCKPLSESPQIPLYRCVNVKEYFTIKAKKEGKGLSAIKI 349

BLAST of MS001278 vs. ExPASy TrEMBL
Match: A0A6J1KKU0 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111496619 PE=3 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 5.2e-145
Identity = 253/347 (72.91%), Postives = 286/347 (82.42%), Query Frame = 0

Query: 1   MADQELPPPSHVVPLDFDSVETVPESHSWPDSNGLSST---DEKVTIPLIDLVDDNASEL 60
           MADQE       +PLDF+SV+TVPESH WPDS  LSS    DEK+++P IDL+DD ASEL
Sbjct: 1   MADQEF------LPLDFNSVQTVPESHLWPDSEELSSVIDLDEKISLPFIDLLDDGASEL 60

Query: 61  IGRACEQWGMFQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVIS 120
           +GRACEQWGMFQLTNHG+ +TLIAE E+EARRLF LP ++KM AL S+D VTGYG AVIS
Sbjct: 61  LGRACEQWGMFQLTNHGISKTLIAEMEEEARRLFTLPAKEKMKALRSADRVTGYGTAVIS 120

Query: 121 KYYQKYMWHEGFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLD 180
           KY+ K MWHEGFTILGSP +DAKKLWPS+ K FCDVMEEYQ QM+ L+D++IRL+FKFLD
Sbjct: 121 KYHPKCMWHEGFTILGSPANDAKKLWPSDSKRFCDVMEEYQSQMKGLSDRLIRLIFKFLD 180

Query: 181 ISEQVIEKLSPSNNDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGL 240
           ISE+   KL  S  D G+ YTAL+LNSYPPCPDP QVMGLAAHTDTSLFTI+HQ   DGL
Sbjct: 181 ISEEETMKLLSSTGDSGKSYTALQLNSYPPCPDPDQVMGLAAHTDTSLFTIVHQVCNDGL 240

Query: 241 QVFKDEVGWIPLSPMSGTLVVNVGDLLHILSNGRFRSILHRVVIQGKE-HRLSMAYFYYP 300
           Q+FKD   W+PLSPM G+ VVNVGDLLHI+SNGRF SILHRV IQGK+ HR S+AYFYYP
Sbjct: 241 QIFKDRASWVPLSPMRGSFVVNVGDLLHIMSNGRFPSILHRVTIQGKKVHRFSLAYFYYP 300

Query: 301 PNELYISPHCNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIKI 344
           P  LYISPHC PLSESPQ PLYRCV V EYF+MKAKN G GLSTIKI
Sbjct: 301 PPYLYISPHCKPLSESPQAPLYRCVSVEEYFTMKAKNNGEGLSTIKI 341

BLAST of MS001278 vs. TAIR 10
Match: AT1G80340.1 (gibberellin 3-oxidase 2 )

HSP 1 Score: 295.8 bits (756), Expect = 4.4e-80
Identity = 146/338 (43.20%), Postives = 218/338 (64.50%), Query Frame = 0

Query: 9   PSHVVPLDFDSVETVPESHSWPDSNGL----SSTDEKVTIPLIDLVDDNASELIGRACEQ 68
           P H+   +    +++P+S++W   + L    S++DE  T+PLIDL D + + L+G AC  
Sbjct: 13  PIHIPLSNPPDFKSLPDSYTWTPKDDLLFSASASDE--TLPLIDLSDIHVATLVGHACTT 72

Query: 69  WGMFQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVISKYYQKYM 128
           WG FQ+TNHGVP  L+ + E     LF LP ++K+ A  S + V+GYG+A I+ ++ K M
Sbjct: 73  WGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYGVARIASFFNKKM 132

Query: 129 WHEGFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLDISEQVIE 188
           W EGFT++GSP+ D +KLWPS +  +C+++EEY+  M+ LA K++      L + E+ I+
Sbjct: 133 WSEGFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFALGSLGVEEKDIQ 192

Query: 189 KLSPSNNDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQVFKDEV 248
              P N+D       ++LN YP CP+P + MGLAAHTD++L TIL+Q    GLQVF+D+V
Sbjct: 193 WAGP-NSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDV 252

Query: 249 GWIPLSPMSGTLVVNVGDLLHILSNGRFRSILHRVVIQGKEHRLSMAYFYYPPNELYISP 308
           GW+   P+ G+LVVNVGDLLHIL+NG F S+LHR  +     R SMAY + PP+++ ISP
Sbjct: 253 GWVTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHVRSRFSMAYLWGPPSDIMISP 312

Query: 309 HCNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIK 343
               L +  Q+PLY  +  ++Y + KA +  + LS I+
Sbjct: 313 -LPKLVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

BLAST of MS001278 vs. TAIR 10
Match: AT1G15550.1 (gibberellin 3-oxidase 1 )

HSP 1 Score: 295.0 bits (754), Expect = 7.5e-80
Identity = 152/342 (44.44%), Postives = 217/342 (63.45%), Query Frame = 0

Query: 8   PPSHVVPLDFDSVETVPESHSWPDSNGL------SSTDEKVTIPLIDLVDDNASELIGRA 67
           P SH+   DF S+  +P+S+ W   + L      S       IPLIDL   +A+  IG A
Sbjct: 17  PHSHIP--DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDHPDATNQIGHA 76

Query: 68  CEQWGMFQLTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVISKYYQ 127
           C  WG FQ++NHGVP  L+ + E     LF LP ++K+ +  S   V+GYG+A I+ ++ 
Sbjct: 77  CRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSGYGVARIASFFN 136

Query: 128 KYMWHEGFTILGSPVDDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLDISEQ 187
           K MW EGFTI GSP++D +KLWP  + ++CD++EEY+  M+ LA K++ L    L +SE+
Sbjct: 137 KQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWLALNSLGVSEE 196

Query: 188 VIEKLSPSNNDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQVFK 247
            IE  S S +D+     AL+LN YP CP+P + MGLAAHTD++L TIL+Q    GLQVF+
Sbjct: 197 DIEWASLS-SDLNWAQAALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGLQVFR 256

Query: 248 DEVGWIPLSPMSGTLVVNVGDLLHILSNGRFRSILHRVVIQGKEHRLSMAYFYYPPNELY 307
           D++GW+ + P  G+LVVNVGDL HILSNG F+S+LHR  +     RLS+A+ + P +++ 
Sbjct: 257 DDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQTRARLSVAFLWGPQSDIK 316

Query: 308 ISPHCNPLSESP-QTPLYRCVKVREYFSMKAKNMGRGLSTIK 343
           ISP   P   SP ++PLY+ V  +EY   KA +  + LS I+
Sbjct: 317 ISP--VPKLVSPVESPLYQSVTWKEYLRTKATHFNKALSMIR 353

BLAST of MS001278 vs. TAIR 10
Match: AT4G21690.1 (gibberellin 3-oxidase 3 )

HSP 1 Score: 266.2 bits (679), Expect = 3.8e-71
Identity = 137/337 (40.65%), Postives = 205/337 (60.83%), Query Frame = 0

Query: 12  VVPLDFDSVETVPESHSWPDSNGLSSTDEKVTIPLIDLVDDNASELIGRACEQWGMFQLT 71
           ++PLDF + +T+P+SH W      +++     IP+I L +     L+ +ACE+WG+F +T
Sbjct: 18  IIPLDFTNTKTLPDSHVWSKPEPETTSG---PIPVISLSNPEEHGLLRQACEEWGVFHIT 77

Query: 72  NHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVISKYYQKYMWHEGFTI 131
           +HGV  +L+   + + +RLF LP  +K++A+ S D  TGYG+  IS +Y K MW EGF++
Sbjct: 78  DHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDESTGYGVVRISMFYDKLMWSEGFSV 137

Query: 132 LGSPV-DDAKKLWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLDISEQVIEKLSPSN 191
           +GS +   A  LWP ++  FC+VMEEYQ  M  L+ ++I ++   L ++ + +  L P  
Sbjct: 138 MGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISMLMGSLGLTHEDLGWLVPDK 197

Query: 192 NDVG--QLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQV-FKDEVG-- 251
              G   + + L+LNSYP CPDP   MGLA HTD+SL TIL+Q  I GL++    E G  
Sbjct: 198 TGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIESPQEEGSR 257

Query: 252 WIPLSPMSGTLVVNVGDLLHILSNGRFRSILHRVVIQGKEHRLSMAYFYYPPNELYISPH 311
           WI + P+ G+LVV +GDL HI+SNG+FRS +HR V+    HR+S AYF  PP  L I P 
Sbjct: 258 WIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVNKTHHRVSAAYFAGPPKNLQIGP- 317

Query: 312 CNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIK 343
               S+    P+YR +   EY + KA +  + L+  +
Sbjct: 318 --LTSDKNHPPIYRRLIWEEYLAAKATHFNKALTLFR 348

BLAST of MS001278 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 251.1 bits (640), Expect = 1.2e-66
Identity = 129/343 (37.61%), Postives = 208/343 (60.64%), Query Frame = 0

Query: 19  SVETVPESHSWP---------DSNGLSSTDEKVTIPLIDLVDDNASELIGRACEQWGMFQ 78
           S++T+PES +W           S+ +S    + +IP+IDL + + + LIG A + WG FQ
Sbjct: 15  SLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQ 74

Query: 79  LTNHGVPETLIAEAEKEARRLFDLPTRQKMMALYSSDSVTGYGMAVISKYYQKYMWHEGF 138
           + NHG+ + L+ + E  ++ LFD+P+ +K+ A  S   V+GYG   IS +++K MW EGF
Sbjct: 75  IANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWSEGF 134

Query: 139 TILGSPVDDAKK-----LWPSEYKHFCDVMEEYQIQMRALADKIIRLVFKFLDISEQVIE 198
           TI     DD+ +     LWP ++  +C +++EY  +M  LA +++  +   L ++ + IE
Sbjct: 135 TI----ADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIE 194

Query: 199 ---KLSPSNNDVGQLYTALRLNSYPPCPDPSQVMGLAAHTDTSLFTILHQARIDGLQVFK 258
              KL  S + VG+   A+RLN YP CP+P + MGLAAHTD+++ TILHQ+   GLQVF+
Sbjct: 195 WAHKLEKSGSKVGR--GAIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFR 254

Query: 259 DEVGWIPLSPMSGTLVVNVGDLLHILSNGRFRSILHRVVIQGKEHRLSMAYFY-YPPNEL 318
           +E GW+ + P  G LVVN+GDL HILSNG+  S++HR  +     R+S+AY +  P  ++
Sbjct: 255 EESGWVTVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRSRISIAYLWGGPAGDV 314

Query: 319 YISPHCNPLSESPQTPLYRCVKVREYFSMKAKNMGRGLSTIKI 344
            I+P  + L+   +  LYR +  +EY  +K +   + +  I++
Sbjct: 315 QIAP-ISKLTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRV 350

BLAST of MS001278 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 182.2 bits (461), Expect = 7.1e-46
Identity = 100/310 (32.26%), Postives = 161/310 (51.94%), Query Frame = 0

Query: 44  IPLIDLV-------DDNASEL--IGRACEQWGMFQLTNHGVPETLIAEAEKEARRLFDLP 103
           IP+IDL        DD   E+  + +ACE WG FQ+ NHG+   ++ + E+ A   FD+P
Sbjct: 55  IPVIDLSKLSKPDNDDFFFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDMP 114

Query: 104 TRQKMMALYSSDSVTGYGMAVISKYYQKYMWHEGFTI-LGSPVDDAKKLWPSEYKHFCDV 163
             +K        +V GYG A I    QK  W   F + +  P     KLWPS+   F + 
Sbjct: 115 LEEKKKYPMEPGTVQGYGQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSES 174

Query: 164 MEEYQIQMRALADKIIRLVFKFLDISEQVIEKLSPSNNDVGQLYTALRLNSYPPCPDPSQ 223
           +E Y  ++R L  ++++ +   L + E+  E++       G+   A+R+N YPPC  P  
Sbjct: 175 LEGYSKEIRELCKRLLKYIAISLGLKEERFEEM------FGEAVQAVRMNYYPPCSSPDL 234

Query: 224 VMGLAAHTDTSLFTILHQARID--GLQVFKDEVGWIPLSPMSGTLVVNVGDLLHILSNGR 283
           V+GL+ H+D S  T+L Q++    GLQ+ KD   W+P+ P+   LV+N+GD + +LSNG+
Sbjct: 235 VLGLSPHSDGSALTVLQQSKNSCVGLQILKDNT-WVPVKPLPNALVINIGDTIEVLSNGK 294

Query: 284 FRSILHRVVIQGKEHRLSMAYFYYPPNELYISPHCNPLSESPQTPLYRCVKVREYFSMKA 342
           ++S+ HR V   ++ RL++  FY P  E+ I P    + +      YR     +Y     
Sbjct: 295 YKSVEHRAVTNREKERLTIVTFYAPNYEVEIEPMSELVDDETNPCKYRSYNHGDYSYHYV 354