MS001253 (gene) Bitter gourd (TR) v1

Overview


Name	MS001253
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	CaM_binding domain-containing protein
Location	scaffold36: 2667099 .. 2668703 (-)
RNA-Seq Expression	MS001253
Synteny	MS001253

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAACAAGTAGAGCAAGTTTTGGGAAGGAAAGGAGGATCACTGCTCCAACTTCTCTCACCATTCCTAATTATTCAACCAAAAGAACTCCAAAATCATCTCCCAGAAGCCAGCAAACTGATCACAAACTAGCTTATTCTTCTTCTTTTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCAACTTCTTCCAAACTAACCTCCTCTTCTTCTTCTCCTTCGCTCTCGATGCTCTCCGAGAAGCACGTTCCGAACTATCTCAAGTCCACAGCCAGCTCCCGCCACGACCATGGCAGCTTCAAGACGGTCAAGAAGTTTGTCGCCCCTGCACCGATACCAGAGGGCAAGGCGAGCTTGAATAGAAGAAGATCTTTCGACAAACCACCCCCGACCGCACCGCGTCTGGATAAGCCGTTTCGGTCACCTGGACCGAGGAGTAGGGCCACTCATGTTCCCGTCCGGTCTTCCTCCTTCGGGTCCAAACCCGCCCCCACCTTTCCCAGCTCTGCAAAGCCTGGCCACTTGGAGAGATCATCATCTTCCAAAACCTCACCCAAGGTCGAGCCTAGCTATTTTGAGAGATCATCATCTGCCAAAACCTCATCCAAGGTTGAGCCAAGCCATTTGGAGAGATCATCATCTTCCAAATCCTCATCCAAGGTTGAGCCTAGCCATTTGGAGAGATCATCATCTGCCAAAACCTCATCCAAGGTTGAGCCAAGCCATTTGGAGAGATCATCATCTTCCAAATCCTCATCCAAGGTGGGTGGGAAACCGCAGCAGCCAGTTCAGAATTTGAGAAGTGGTAGTAATGCAATTGCAAAGAAAAGCTTGAGGAGAGAATCTAGCAATGCTGGCTCTGCCTTGGCAGAAATAGTTCCCAAGGCTAAGAATATTGCTGTTGAACAAGCAGTTCATAGTCCTTCTTTCGTTCTCGGTGTCAACGAGGAGGAATTGAAGAAGATCGAGTGCGAACTCGATCCTTATCTTCCTGATCCAATGCCTGAATTAGATAAAGAACTCAGAACTGATCATGTGGAAAAAAAGGTGGATGTTTTGGATAAAGAAGAAGCTGATGCAAAACATGAGGCTGAGAAGCAGCCTATACAAGACACAGAGATCTCCAAAGCTGACACTGAAACTCCATCGCCTATAGAGGTGGAAGCGAAATTGTTAATCCAGGAAGAATCGAATCGAGAACGAACGGAAGGTGTAGGAGAGGAAGAAGTCAAAGATGAGGAGAAACCAGAGATAATAACACAAGAAAAAGAGGTAGATAAGGATGAAGAGAAAAGCGAGATCAGTCCTACTCAGGAAGGAATCGTCGTTTTAGATCAGAAAGAAGTAGAAGATGGGGAGAATTGCAAGGAAGAGGCTGAAGCGACCGAGAAGGTGGCAGCAGCGGAGAAAGAGGGCGAGGCAGTGGCCGAGAAGCTGGCAGTGATGGGAGCGAAACAACGACAAGGAAGCGGGAGGAAGGAGTCTCCGGCAATGTACAATGATGTAATAGAGGAGACAGCAAGTAAGCTATTGGAGAAGAGGCAGAACAAGGTAAAAGCATTGGCTGGGGCATTTCAGACAGTCATTGACTATGAATCTTCATCCAAA

mRNA sequence

ATGGCAACAAGTAGAGCAAGTTTTGGGAAGGAAAGGAGGATCACTGCTCCAACTTCTCTCACCATTCCTAATTATTCAACCAAAAGAACTCCAAAATCATCTCCCAGAAGCCAGCAAACTGATCACAAACTAGCTTATTCTTCTTCTTTTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCAACTTCTTCCAAACTAACCTCCTCTTCTTCTTCTCCTTCGCTCTCGATGCTCTCCGAGAAGCACGTTCCGAACTATCTCAAGTCCACAGCCAGCTCCCGCCACGACCATGGCAGCTTCAAGACGGTCAAGAAGTTTGTCGCCCCTGCACCGATACCAGAGGGCAAGGCGAGCTTGAATAGAAGAAGATCTTTCGACAAACCACCCCCGACCGCACCGCGTCTGGATAAGCCGTTTCGGTCACCTGGACCGAGGAGTAGGGCCACTCATGTTCCCGTCCGGTCTTCCTCCTTCGGGTCCAAACCCGCCCCCACCTTTCCCAGCTCTGCAAAGCCTGGCCACTTGGAGAGATCATCATCTTCCAAAACCTCACCCAAGGTGGGTGGGAAACCGCAGCAGCCAGTTCAGAATTTGAGAAGTGGTAGTAATGCAATTGCAAAGAAAAGCTTGAGGAGAGAATCTAGCAATGCTGGCTCTGCCTTGGCAGAAATAGTTCCCAAGGCTAAGAATATTGCTGTTGAACAAGCAGTTCATAGTCCTTCTTTCGTTCTCGGTGTCAACGAGGAGGAATTGAAGAAGATCGAGTGCGAACTCGATCCTTATCTTCCTGATCCAATGCCTGAATTAGATAAAGAACTCAGAACTGATCATGTGGAAAAAAAGGTGGATGTTTTGGATAAAGAAGAAGCTGATGCAAAACATGAGGCTGAGAAGCAGCCTATACAAGACACAGAGATCTCCAAAGCTGACACTGAAACTCCATCGCCTATAGAGGTGGAAGCGAAATTGTTAATCCAGGAAGAATCGAATCGAGAACGAACGGAAGGTGTAGGAGAGGAAGAAGTCAAAGATGAGGAGAAACCAGAGATAATAACACAAGAAAAAGAGGTAGATAAGGATGAAGAGAAAAGCGAGATCAGTCCTACTCAGGAAGGAATCGTCGTTTTAGATCAGAAAGAAGTAGAAGATGGGGAGAATTGCAAGGAAGAGGCTGAAGCGACCGAGAAGGTGGCAGCAGCGGAGAAAGAGGGCGAGGCAGTGGCCGAGAAGCTGGCAGTGATGGGAGCGAAACAACGACAAGGAAGCGGGAGGAAGGAGTCTCCGGCAATGTACAATGATGTAATAGAGGAGACAGCAAGTAAGCTATTGGAGAAGAGGCAGAACAAGGTAAAAGCATTGGCTGGGGCATTTCAGACAGTCATTGACTATGAATCTTCATCCAAA

Coding sequence (CDS)

ATGGCAACAAGTAGAGCAAGTTTTGGGAAGGAAAGGAGGATCACTGCTCCAACTTCTCTCACCATTCCTAATTATTCAACCAAAAGAACTCCAAAATCATCTCCCAGAAGCCAGCAAACTGATCACAAACTAGCTTATTCTTCTTCTTTTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCAACTTCTTCCAAACTAACCTCCTCTTCTTCTTCTCCTTCGCTCTCGATGCTCTCCGAGAAGCACGTTCCGAACTATCTCAAGTCCACAGCCAGCTCCCGCCACGACCATGGCAGCTTCAAGACGGTCAAGAAGTTTGTCGCCCCTGCACCGATACCAGAGGGCAAGGCGAGCTTGAATAGAAGAAGATCTTTCGACAAACCACCCCCGACCGCACCGCGTCTGGATAAGCCGTTTCGGTCACCTGGACCGAGGAGTAGGGCCACTCATGTTCCCGTCCGGTCTTCCTCCTTCGGGTCCAAACCCGCCCCCACCTTTCCCAGCTCTGCAAAGCCTGGCCACTTGGAGAGATCATCATCTTCCAAAACCTCACCCAAGGTGGGTGGGAAACCGCAGCAGCCAGTTCAGAATTTGAGAAGTGGTAGTAATGCAATTGCAAAGAAAAGCTTGAGGAGAGAATCTAGCAATGCTGGCTCTGCCTTGGCAGAAATAGTTCCCAAGGCTAAGAATATTGCTGTTGAACAAGCAGTTCATAGTCCTTCTTTCGTTCTCGGTGTCAACGAGGAGGAATTGAAGAAGATCGAGTGCGAACTCGATCCTTATCTTCCTGATCCAATGCCTGAATTAGATAAAGAACTCAGAACTGATCATGTGGAAAAAAAGGTGGATGTTTTGGATAAAGAAGAAGCTGATGCAAAACATGAGGCTGAGAAGCAGCCTATACAAGACACAGAGATCTCCAAAGCTGACACTGAAACTCCATCGCCTATAGAGGTGGAAGCGAAATTGTTAATCCAGGAAGAATCGAATCGAGAACGAACGGAAGGTGTAGGAGAGGAAGAAGTCAAAGATGAGGAGAAACCAGAGATAATAACACAAGAAAAAGAGGTAGATAAGGATGAAGAGAAAAGCGAGATCAGTCCTACTCAGGAAGGAATCGTCGTTTTAGATCAGAAAGAAGTAGAAGATGGGGAGAATTGCAAGGAAGAGGCTGAAGCGACCGAGAAGGTGGCAGCAGCGGAGAAAGAGGGCGAGGCAGTGGCCGAGAAGCTGGCAGTGATGGGAGCGAAACAACGACAAGGAAGCGGGAGGAAGGAGTCTCCGGCAATGTACAATGATGTAATAGAGGAGACAGCAAGTAAGCTATTGGAGAAGAGGCAGAACAAGGTAAAAGCATTGGCTGGGGCATTTCAGACAGTCATTGACTATGAATCTTCATCCAAA

Protein sequence

MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSFSSSSSSSSSSTSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNRRRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSSKTSPKVGGKPQQPVQNLRSGSNAIAKKSLRRESSNAGSALAEIVPKAKNIAVEQAVHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKELRTDHVEKKVDVLDKEEADAKHEAEKQPIQDTEISKADTETPSPIEVEAKLLIQEESNRERTEGVGEEEVKDEEKPEIITQEKEVDKDEEKSEISPTQEGIVVLDQKEVEDGENCKEEAEATEKVAAAEKEGEAVAEKLAVMGAKQRQGSGRKESPAMYNDVIEETASKLLEKRQNKVKALAGAFQTVIDYESSSK

Homology

[Close]

BLAST of MS001253 vs. NCBI nr
Match: XP_022131479.1 (uncharacterized protein DDB_G0271670 isoform X2 [Momordica charantia])

HSP 1 Score: 799.3 bits (2063), Expect = 1.8e-227
Identity = 463/518 (89.38%), Postives = 464/518 (89.58%), Query Frame = 0


Query: 1   MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSFSSSSSSSSSS 60
           MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSF  SSSSSSSS
Sbjct: 1   MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSF--SSSSSSSS 60

Query: 61  TSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNR 120
           TSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNR
Sbjct: 61  TSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNR 120

Query: 121 RRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSS 180
           RRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSS
Sbjct: 121 RRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSS 180

Query: 181 KTSP---------------------------------------------------KVGGK 240
           KTSP                                                   KVGGK
Sbjct: 181 KTSPKVEPSYLERSSSAKTSSKVEPSHLERSSSSKSSSKVEPSHLERSSSSKSSSKVGGK 240

Query: 241 PQQPVQNLRSGSNAIAKKSLRRESSNAGSALAEIVPKAKNIAVEQAVHSPSFVLGVNEEE 300
           PQQPVQNLRSGSNAIAKKSLRRESSNAGSALAEIVPKAKNIAVEQAVHSPSFVLGVNEEE
Sbjct: 241 PQQPVQNLRSGSNAIAKKSLRRESSNAGSALAEIVPKAKNIAVEQAVHSPSFVLGVNEEE 300

Query: 301 LKKIECELDPYLPDPMPELDKELRTDHVEKKVDVLDKEEADAKHEAEKQPIQDTEISKAD 360
           LKKIECELDPYLPDPMPELDKE+RTDHVEKKVDVLDKEEADAKHEAEKQPIQDTEISKAD
Sbjct: 301 LKKIECELDPYLPDPMPELDKEIRTDHVEKKVDVLDKEEADAKHEAEKQPIQDTEISKAD 360

Query: 361 TETPSPIEVEAKLLIQEESNRERTEGVGEEEVKDEEKPEIITQEKEVDKDEEKSEISPTQ 420
           TETPSPIEVEAKLLIQEESNRERTEGVGEEEVKDEEKPEIITQEKEVDKDEEKSEISPTQ
Sbjct: 361 TETPSPIEVEAKLLIQEESNRERTEGVGEEEVKDEEKPEIITQEKEVDKDEEKSEISPTQ 420

Query: 421 EGIVVLDQKEVEDGENCKEEAEATEKVAAAEKEGEAVAEKLAVMGAKQRQGSGRKESPAM 468
           EGIVVLDQKEVEDGENCKEEAE TEKVAAAEKEGEAVAEKLAVMGAKQRQGSGRKESPAM
Sbjct: 421 EGIVVLDQKEVEDGENCKEEAEVTEKVAAAEKEGEAVAEKLAVMGAKQRQGSGRKESPAM 480

[Close]

BLAST of MS001253 vs. NCBI nr
Match: XP_022131477.1 (uncharacterized protein DDB_G0271670 isoform X1 [Momordica charantia] >XP_022131478.1 uncharacterized protein DDB_G0271670 isoform X1 [Momordica charantia])

HSP 1 Score: 786.2 bits (2029), Expect = 1.6e-223
Identity = 463/552 (83.88%), Postives = 464/552 (84.06%), Query Frame = 0


Query: 1   MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSFSSSSSSSSSS 60
           MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSF  SSSSSSSS
Sbjct: 1   MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSF--SSSSSSSS 60

Query: 61  TSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNR 120
           TSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNR
Sbjct: 61  TSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNR 120

Query: 121 RRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSS 180
           RRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSS
Sbjct: 121 RRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSS 180

Query: 181 KTSP-------------------------------------------------------- 240
           KTSP                                                        
Sbjct: 181 KTSPKVEPSYLERSSSAKTSSKVEPSHLERSSSSKSSSKVEPSHLERSSSAKTSSKVEPS 240

Query: 241 -----------------------------KVGGKPQQPVQNLRSGSNAIAKKSLRRESSN 300
                                        KVGGKPQQPVQNLRSGSNAIAKKSLRRESSN
Sbjct: 241 HLERSSSSKSSSKVEPSHLERSSSSKSSSKVGGKPQQPVQNLRSGSNAIAKKSLRRESSN 300

Query: 301 AGSALAEIVPKAKNIAVEQAVHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKELRTD 360
           AGSALAEIVPKAKNIAVEQAVHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKE+RTD
Sbjct: 301 AGSALAEIVPKAKNIAVEQAVHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKEIRTD 360

Query: 361 HVEKKVDVLDKEEADAKHEAEKQPIQDTEISKADTETPSPIEVEAKLLIQEESNRERTEG 420
           HVEKKVDVLDKEEADAKHEAEKQPIQDTEISKADTETPSPIEVEAKLLIQEESNRERTEG
Sbjct: 361 HVEKKVDVLDKEEADAKHEAEKQPIQDTEISKADTETPSPIEVEAKLLIQEESNRERTEG 420

Query: 421 VGEEEVKDEEKPEIITQEKEVDKDEEKSEISPTQEGIVVLDQKEVEDGENCKEEAEATEK 468
           VGEEEVKDEEKPEIITQEKEVDKDEEKSEISPTQEGIVVLDQKEVEDGENCKEEAE TEK
Sbjct: 421 VGEEEVKDEEKPEIITQEKEVDKDEEKSEISPTQEGIVVLDQKEVEDGENCKEEAEVTEK 480

[Close]

BLAST of MS001253 vs. NCBI nr
Match: KAG7029749.1 (hypothetical protein SDJN02_08091, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 417.2 bits (1071), Expect = 1.9e-112
Identity = 306/504 (60.71%), Postives = 341/504 (67.66%), Query Frame = 0


Query: 1   MATSRA----SFGKERRITAPTSLTIPNY--STKRTPKSSPRSQQTDHKLAYSSSFSSSS 60
           MATSRA      GKER+ITAP+S+TIPN   STKRTPKSSPR   TD KL   SSF SS 
Sbjct: 1   MATSRAKESCGLGKERKITAPSSVTIPNLNTSTKRTPKSSPRGISTDQKL---SSFPSSP 60

Query: 61  SSSSSSTSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEG 120
           SSS         SSSS+ SLS+L +KHVPNYLKSTASSRHDH +FK +KK   PAPIPEG
Sbjct: 61  SSS---------SSSSTHSLSILPQKHVPNYLKSTASSRHDH-NFKPIKKSGGPAPIPEG 120

Query: 121 KASLNRRRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHL 180
           K SLNRRRSFDKPPP   RL KPFRSP PR+RA H PVRSSSFG+KP+ +  SS K G L
Sbjct: 121 KPSLNRRRSFDKPPPATQRLGKPFRSPEPRNRAAHAPVRSSSFGAKPSTSIVSSTKTGFL 180

Query: 181 ERSSSSKTSPKVGGKPQQPVQNLRSGSNAIAKKSLRRESSNAGSA-LAEIVPKAKNIAVE 240
           ERS S     KVGGK QQ VQ L+S SN   +KSL+RESSNA SA  AE+VPK KN AVE
Sbjct: 181 ERSGSK--IYKVGGKTQQSVQTLKSSSN--VRKSLKRESSNAASASSAEMVPKTKN-AVE 240

Query: 241 QAVHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKEL-RTDHVEKKVDVLDKEEADAK 300
               +P  VLG NEE+LKKIECELD  LPDP PELDKEL  TD VEK++           
Sbjct: 241 HVGQTPVLVLGANEEDLKKIECELDLCLPDPRPELDKELSTTDQVEKRLH---------- 300

Query: 301 HEAEKQPIQDTEI------SKADTETPSPIEVE-AKLLIQEESNRERTEGVGEEEVKDEE 360
                QPIQDT+I      +K   ETP   E + AK  IQE+ NRE+ EG GEEE K+EE
Sbjct: 301 ---HHQPIQDTKIFKVVGSNKKVEETPGAAETDNAKSSIQEDENREQNEGEGEEEAKEEE 360

Query: 361 KPEIITQEKEV-------------DKDEEKSEISPTQEGIVVLDQKEVEDGENCKE---- 420
           KPE I  EKE                +EEKS   P QEG++VL+ KEVEDG++ KE    
Sbjct: 361 KPERIEVEKEEVFGVVASEESRNNKNEEEKSHTDPIQEGVIVLEHKEVEDGQSSKEENHN 420

Query: 421 ----EAEATEKVAAAEKEGEAVAEKLAVMGAKQRQGS-GRKESPAMYNDVIEETASKLLE 468
               EAEATEKVAAA     A AE  A   AK RQG  GRKESPA+YN VIEETASKLLE
Sbjct: 421 STEKEAEATEKVAAA---ATATAEAAA---AKPRQGGPGRKESPAVYNHVIEETASKLLE 467

[Close]

BLAST of MS001253 vs. NCBI nr
Match: XP_023546370.1 (cell wall protein RBR3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 416.4 bits (1069), Expect = 3.3e-112
Identity = 303/504 (60.12%), Postives = 337/504 (66.87%), Query Frame = 0


Query: 1   MATSRA----SFGKERRITAPTSLTIPNY--STKRTPKSSPRSQQTDHKLAYSSSFSSSS 60
           MATSRA      GKER+ITAP+S+TIPN   STKRTPKSSPR   TD KL   SSF SS 
Sbjct: 1   MATSRAKESCGLGKERKITAPSSVTIPNVNTSTKRTPKSSPRGISTDQKL---SSFPSSP 60

Query: 61  SSSSSSTSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEG 120
           SSS         SSSS+ SLS+L +KHVPNYLKSTASSRHDH +FK +KK   P PIPEG
Sbjct: 61  SSS---------SSSSTHSLSILPQKHVPNYLKSTASSRHDH-NFKPIKKSAGPTPIPEG 120

Query: 121 KASLNRRRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHL 180
           K SLNRRRSFDKPPP   RL KPFRSP PR+RA H PVRSSSFG+KP+ T  SS K G L
Sbjct: 121 KPSLNRRRSFDKPPPATQRLGKPFRSPEPRNRAAHAPVRSSSFGAKPSTTIVSSTKTGFL 180

Query: 181 ERSSSSKTSPKVGGKPQQPVQNLRSGSNAIAKKSLRRESSNAGSA-LAEIVPKAKNIAVE 240
           ERS S     KVGGK QQ VQ L+S SN   +KSL+RESSNA SA  AE+VPK KN AVE
Sbjct: 181 ERSGSK--IYKVGGKTQQSVQTLKSSSN--VRKSLKRESSNAASASSAEMVPKTKN-AVE 240

Query: 241 QAVHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKEL-RTDHVEKKVDVLDKEEADAK 300
               SP  VLG NEE+LKKIECELD  LPDP PELDKEL  TD VEK++           
Sbjct: 241 HVGQSPVLVLGANEEDLKKIECELDLCLPDPRPELDKELSTTDQVEKRLH---------- 300

Query: 301 HEAEKQPIQDTEI------SKADTETPSPIEVE-AKLLIQEESNRERTEGVGEEEVKDEE 360
                QPIQDT+I      +K   ETP   E + AK  I+E+ NRE+ EG GEEE K+EE
Sbjct: 301 ---HHQPIQDTKIFKVVGSNKKVEETPGAAETDNAKSSIREDENREQNEGEGEEEAKEEE 360

Query: 361 KPEIITQEKEV-------------DKDEEKSEISPTQEGIVVLDQKEVEDGENCKE---- 420
           KPE I  EKE                +EEKS   P Q G++VL+ KEVEDG++ KE    
Sbjct: 361 KPERIEVEKEEVFGVVASEESTNNKNEEEKSHTDPIQVGVIVLEHKEVEDGQSSKEENHN 420

Query: 421 ----EAEATEKVAAAEKEGEAVAEKLAVMGAKQRQGS-GRKESPAMYNDVIEETASKLLE 468
               EAEATEKVAAA     A A       AK RQG  GRKESPA+YN VIEETASKLLE
Sbjct: 421 STEKEAEATEKVAAA-----AAAAAAEAAAAKPRQGGPGRKESPAVYNHVIEETASKLLE 468

[Close]

BLAST of MS001253 vs. NCBI nr
Match: KAG6598809.1 (hypothetical protein SDJN03_08587, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 415.6 bits (1067), Expect = 5.6e-112
Identity = 305/504 (60.52%), Postives = 341/504 (67.66%), Query Frame = 0


Query: 1   MATSRA----SFGKERRITAPTSLTIPNY--STKRTPKSSPRSQQTDHKLAYSSSFSSSS 60
           MATSRA      GKER+ITAP+S+TIPN   STKRTPKSSPR   TD KL   SSF SS 
Sbjct: 1   MATSRAKESCGLGKERKITAPSSVTIPNLNTSTKRTPKSSPRGISTDQKL---SSFPSSP 60

Query: 61  SSSSSSTSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEG 120
           SSS         SSSS+ SLS+L +KHVPNYLKSTASSRHDH +FK +KK   PAPIPEG
Sbjct: 61  SSS---------SSSSTHSLSILPQKHVPNYLKSTASSRHDH-NFKPIKKSGGPAPIPEG 120

Query: 121 KASLNRRRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHL 180
           K SLNRRRSFDKPPP   RL KPFRSP PR+RA H PVRSSSFG+KP+ +  SS K G L
Sbjct: 121 KPSLNRRRSFDKPPPATQRLGKPFRSPEPRNRAAHAPVRSSSFGAKPSTSIVSSTKTGFL 180

Query: 181 ERSSSSKTSPKVGGKPQQPVQNLRSGSNAIAKKSLRRESSNAGSA-LAEIVPKAKNIAVE 240
           ERS S     KVGGK QQ VQ L+S SN   +KSL+RESSNA SA  AE+VPK KN AVE
Sbjct: 181 ERSGSK--IYKVGGKTQQSVQTLKSSSN--VRKSLKRESSNAASASSAEMVPKTKN-AVE 240

Query: 241 QAVHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKEL-RTDHVEKKVDVLDKEEADAK 300
               +P  VLG NEE+LKKIECELD  LPDP PELDKEL  TD VEK++           
Sbjct: 241 HVGQTPVLVLGANEEDLKKIECELDLCLPDPRPELDKELSTTDQVEKRLH---------- 300

Query: 301 HEAEKQPIQDTEI------SKADTETPSPIEVE-AKLLIQEESNRERTEGVGEEEVKDEE 360
                QPIQDT+I      +K   ETP   E + AK  I+E+ NRE+ EG GEEE K+EE
Sbjct: 301 ---HHQPIQDTKIFKVVGSNKKVEETPGAAETDNAKSSIREDENREQNEGEGEEEAKEEE 360

Query: 361 KPEIITQEKEV-------------DKDEEKSEISPTQEGIVVLDQKEVEDGENCKE---- 420
           KPE I  EKE                +EEKS   P QEG++VL+ KEVEDG++ KE    
Sbjct: 361 KPERIEVEKEEVFGVVASEESTNNKNEEEKSHTDPIQEGVIVLEHKEVEDGQSSKEENHN 420

Query: 421 ----EAEATEKVAAAEKEGEAVAEKLAVMGAKQRQGS-GRKESPAMYNDVIEETASKLLE 468
               EAEATEKVAAA     A AE  A   AK RQG  GRKESPA+YN VIEETASKLLE
Sbjct: 421 STEKEAEATEKVAAA---ATAAAEAAA---AKPRQGGPGRKESPAVYNHVIEETASKLLE 467

[Close]

BLAST of MS001253 vs. ExPASy TrEMBL
Match: A0A6J1BQC8 (uncharacterized protein DDB_G0271670 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111004674 PE=4 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 8.7e-228
Identity = 463/518 (89.38%), Postives = 464/518 (89.58%), Query Frame = 0


Query: 1   MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSFSSSSSSSSSS 60
           MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSF  SSSSSSSS
Sbjct: 1   MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSF--SSSSSSSS 60

Query: 61  TSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNR 120
           TSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNR
Sbjct: 61  TSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNR 120

Query: 121 RRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSS 180
           RRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSS
Sbjct: 121 RRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSS 180

Query: 181 KTSP---------------------------------------------------KVGGK 240
           KTSP                                                   KVGGK
Sbjct: 181 KTSPKVEPSYLERSSSAKTSSKVEPSHLERSSSSKSSSKVEPSHLERSSSSKSSSKVGGK 240

Query: 241 PQQPVQNLRSGSNAIAKKSLRRESSNAGSALAEIVPKAKNIAVEQAVHSPSFVLGVNEEE 300
           PQQPVQNLRSGSNAIAKKSLRRESSNAGSALAEIVPKAKNIAVEQAVHSPSFVLGVNEEE
Sbjct: 241 PQQPVQNLRSGSNAIAKKSLRRESSNAGSALAEIVPKAKNIAVEQAVHSPSFVLGVNEEE 300

Query: 301 LKKIECELDPYLPDPMPELDKELRTDHVEKKVDVLDKEEADAKHEAEKQPIQDTEISKAD 360
           LKKIECELDPYLPDPMPELDKE+RTDHVEKKVDVLDKEEADAKHEAEKQPIQDTEISKAD
Sbjct: 301 LKKIECELDPYLPDPMPELDKEIRTDHVEKKVDVLDKEEADAKHEAEKQPIQDTEISKAD 360

Query: 361 TETPSPIEVEAKLLIQEESNRERTEGVGEEEVKDEEKPEIITQEKEVDKDEEKSEISPTQ 420
           TETPSPIEVEAKLLIQEESNRERTEGVGEEEVKDEEKPEIITQEKEVDKDEEKSEISPTQ
Sbjct: 361 TETPSPIEVEAKLLIQEESNRERTEGVGEEEVKDEEKPEIITQEKEVDKDEEKSEISPTQ 420

Query: 421 EGIVVLDQKEVEDGENCKEEAEATEKVAAAEKEGEAVAEKLAVMGAKQRQGSGRKESPAM 468
           EGIVVLDQKEVEDGENCKEEAE TEKVAAAEKEGEAVAEKLAVMGAKQRQGSGRKESPAM
Sbjct: 421 EGIVVLDQKEVEDGENCKEEAEVTEKVAAAEKEGEAVAEKLAVMGAKQRQGSGRKESPAM 480

[Close]

BLAST of MS001253 vs. ExPASy TrEMBL
Match: A0A6J1BTH3 (uncharacterized protein DDB_G0271670 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004674 PE=4 SV=1)

HSP 1 Score: 786.2 bits (2029), Expect = 7.6e-224
Identity = 463/552 (83.88%), Postives = 464/552 (84.06%), Query Frame = 0


Query: 1   MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSFSSSSSSSSSS 60
           MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSF  SSSSSSSS
Sbjct: 1   MATSRASFGKERRITAPTSLTIPNYSTKRTPKSSPRSQQTDHKLAYSSSF--SSSSSSSS 60

Query: 61  TSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNR 120
           TSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNR
Sbjct: 61  TSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEGKASLNR 120

Query: 121 RRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSS 180
           RRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSS
Sbjct: 121 RRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHLERSSSS 180

Query: 181 KTSP-------------------------------------------------------- 240
           KTSP                                                        
Sbjct: 181 KTSPKVEPSYLERSSSAKTSSKVEPSHLERSSSSKSSSKVEPSHLERSSSAKTSSKVEPS 240

Query: 241 -----------------------------KVGGKPQQPVQNLRSGSNAIAKKSLRRESSN 300
                                        KVGGKPQQPVQNLRSGSNAIAKKSLRRESSN
Sbjct: 241 HLERSSSSKSSSKVEPSHLERSSSSKSSSKVGGKPQQPVQNLRSGSNAIAKKSLRRESSN 300

Query: 301 AGSALAEIVPKAKNIAVEQAVHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKELRTD 360
           AGSALAEIVPKAKNIAVEQAVHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKE+RTD
Sbjct: 301 AGSALAEIVPKAKNIAVEQAVHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKEIRTD 360

Query: 361 HVEKKVDVLDKEEADAKHEAEKQPIQDTEISKADTETPSPIEVEAKLLIQEESNRERTEG 420
           HVEKKVDVLDKEEADAKHEAEKQPIQDTEISKADTETPSPIEVEAKLLIQEESNRERTEG
Sbjct: 361 HVEKKVDVLDKEEADAKHEAEKQPIQDTEISKADTETPSPIEVEAKLLIQEESNRERTEG 420

Query: 421 VGEEEVKDEEKPEIITQEKEVDKDEEKSEISPTQEGIVVLDQKEVEDGENCKEEAEATEK 468
           VGEEEVKDEEKPEIITQEKEVDKDEEKSEISPTQEGIVVLDQKEVEDGENCKEEAE TEK
Sbjct: 421 VGEEEVKDEEKPEIITQEKEVDKDEEKSEISPTQEGIVVLDQKEVEDGENCKEEAEVTEK 480

[Close]

BLAST of MS001253 vs. ExPASy TrEMBL
Match: A0A6J1HE16 (neurofilament medium polypeptide-like OS=Cucurbita moschata OX=3662 GN=LOC111462632 PE=4 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 6.7e-111
Identity = 302/504 (59.92%), Postives = 340/504 (67.46%), Query Frame = 0


Query: 1   MATSRA----SFGKERRITAPTSLTIPNY--STKRTPKSSPRSQQTDHKLAYSSSFSSSS 60
           MATSRA      GKER+ITAP+S+TIPN   STKRTPKSSPR   TD KL   SSF SS 
Sbjct: 1   MATSRAKESCGLGKERKITAPSSVTIPNLNTSTKRTPKSSPRGISTDQKL---SSFPSSP 60

Query: 61  SSSSSSTSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEG 120
           SSS         SSSS+ SLS+L +KHVPNYLKSTASSRHDH +FK +KK   PAPIPEG
Sbjct: 61  SSS---------SSSSTHSLSILPQKHVPNYLKSTASSRHDH-NFKPIKKSGGPAPIPEG 120

Query: 121 KASLNRRRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHL 180
           K SLNRRRSFDKPPP   RL KPFRSP PR+RA H PVRSSSFG+KP+ +  SS K G L
Sbjct: 121 KPSLNRRRSFDKPPPATQRLGKPFRSPEPRNRAAHAPVRSSSFGAKPSTSIVSSTKTGFL 180

Query: 181 ERSSSSKTSPKVGGKPQQPVQNLRSGSNAIAKKSLRRESSNAGSA-LAEIVPKAKNIAVE 240
           ERS S     KVGGK QQ VQ L+S SN   +KSL+RESSNA SA  AE+VPK KN AVE
Sbjct: 181 ERSGSK--IYKVGGKTQQSVQTLKSSSN--VRKSLKRESSNAASASSAEMVPKTKN-AVE 240

Query: 241 QAVHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKEL-RTDHVEKKVDVLDKEEADAK 300
               SP  VLG NEE+LKKIECELD  LPDP PELDKEL  TD VEK++           
Sbjct: 241 HVGQSPVLVLGANEEDLKKIECELDLCLPDPRPELDKELSTTDQVEKRLH---------- 300

Query: 301 HEAEKQPIQDTEI------SKADTETPSPIEVE-AKLLIQEESNRERTEGVGEEEVKDEE 360
                QPIQ+T+I      +K   ETP   E + AK  I+E+ NRE+ EG GEEE K+EE
Sbjct: 301 ---HHQPIQETKIFKVVGSNKKVEETPGAAETDNAKSSIREDENREQNEGEGEEEAKEEE 360

Query: 361 KPEIITQEKEV-------------DKDEEKSEISPTQEGIVVLDQKEVEDGENCKE---- 420
           KPE I  EKE                +EEKS   P QEG++VL+ KE EDG++ KE    
Sbjct: 361 KPERIEVEKEEVFGVVASEESTNNKNEEEKSHTVPIQEGVIVLEHKEAEDGQSSKEENHT 420

Query: 421 ----EAEATEKVAAAEKEGEAVAEKLAVMGAKQRQGS-GRKESPAMYNDVIEETASKLLE 468
               E EATEKVAAA     + AE +A   AK RQG  GRKESPA+YN VIEETASKLLE
Sbjct: 421 STEKETEATEKVAAA---ATSAAEAVA---AKPRQGGPGRKESPAVYNHVIEETASKLLE 467

[Close]

BLAST of MS001253 vs. ExPASy TrEMBL
Match: A0A6J1K599 (microtubule-associated protein RP/EB family member 1-like OS=Cucurbita maxima OX=3661 GN=LOC111492415 PE=4 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 1.5e-110
Identity = 302/504 (59.92%), Postives = 335/504 (66.47%), Query Frame = 0


Query: 1   MATSRA----SFGKERRITAPTSLTI--PNYSTKRTPKSSPRSQQTDHKLAYSSSFSSSS 60
           MATSRA      GKER+IT P+S+TI  PN STKRTPKSSPR   TD KL   SSF SS 
Sbjct: 1   MATSRAKESCGLGKERKITTPSSVTIPNPNTSTKRTPKSSPRGISTDQKL---SSFPSSP 60

Query: 61  SSSSSSTSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPEG 120
           S SSSST           SLS+L +KHVPNYLKSTASSRHDH +FK +KK   PAPIPEG
Sbjct: 61  SPSSSSTH----------SLSILPQKHVPNYLKSTASSRHDH-NFKPIKKSAGPAPIPEG 120

Query: 121 KASLNRRRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPSSAKPGHL 180
           K SLNRRRSFDKPPP   RL KPFRSP PR+RA H PVRSSSFG+KP+ T  SS K G L
Sbjct: 121 KPSLNRRRSFDKPPPAMQRLGKPFRSPEPRNRAAHAPVRSSSFGAKPSTTLVSSTKTGFL 180

Query: 181 ERSSSSKTSPKVGGKPQQPVQNLRSGSNAIAKKSLRRESSNAGSA-LAEIVPKAKNIAVE 240
           ERS S     KVGGK QQ VQ  +S SN   +KSL+RESSNA SA  AE+VPK KN AVE
Sbjct: 181 ERSGSK--IYKVGGKTQQSVQTFKSSSN--LRKSLKRESSNAASASSAEMVPKTKN-AVE 240

Query: 241 QAVHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKEL-RTDHVEKKVDVLDKEEADAK 300
               SP  VLGVNEE+LKKIECELD  LPDP PELDKEL  TD VEK++           
Sbjct: 241 HVGQSPVLVLGVNEEDLKKIECELDLCLPDPRPELDKELSTTDQVEKRLH---------- 300

Query: 301 HEAEKQPIQDTEI------SKADTETPSPIEVE-AKLLIQEESNRERTEGVGEEEVKDEE 360
                QPIQDT+I      +K   ETP   E + AK  I E+ N+E+ EG GEEE K+EE
Sbjct: 301 ---HHQPIQDTKIFKVVGSNKKVEETPGAAETDNAKSSIWEDENQEQNEGEGEEEAKEEE 360

Query: 361 KPEIITQEKEV-------------DKDEEKSEISPTQEGIVVLDQKEVEDGENCKE---- 420
           KPE I  EKE                +EEKS   P QEG++VL+ KE EDG++ KE    
Sbjct: 361 KPERIEVEKEEVYGVVASEESTNNKNEEEKSHTDPIQEGVIVLEHKEAEDGQSSKEENHN 420

Query: 421 ----EAEATEKVAAAEKEGEAVAEKLAVMGAKQRQGS-GRKESPAMYNDVIEETASKLLE 468
               EAEATEKVAAA     A AE      AK RQG  GRKESPA+YN VIEETASKLLE
Sbjct: 421 STEKEAEATEKVAAA---AAAAAE-----AAKPRQGGPGRKESPAVYNHVIEETASKLLE 464

[Close]

BLAST of MS001253 vs. ExPASy TrEMBL
Match: A0A0A0LS85 (CaM_binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G378000 PE=4 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 1.1e-102
Identity = 285/498 (57.23%), Postives = 334/498 (67.07%), Query Frame = 0


Query: 1   MATSRA----SFGKERRITAPTSLTIP-NYSTKRTPKSSPRS--QQTDHKLAYSSSFSSS 60
           MAT+R+      GKER+IT P+S+TIP N STKRTPKSSPR     TD K     SFS S
Sbjct: 1   MATTRSKESCGLGKERKITPPSSVTIPSNTSTKRTPKSSPRGIISSTDPKF---PSFSPS 60

Query: 61  SSSSSSSTSSKLTSSSSSPSLSMLSEKHVPNYLKSTASSRHDHGSFKTVKKFVAPAPIPE 120
           S+ SS         SS+  SLS+L EKHVPNYLKSTASSR+DH      +    PAPIPE
Sbjct: 61  SAPSS--------PSSAPSSLSILPEKHVPNYLKSTASSRNDHNFKPIARSKSGPAPIPE 120

Query: 121 GKASLNRRRSFDKPPPTAPRLDKPFRSPGPRSRATHVPVRSSSFGSKPAPTFPS---SAK 180
           G+ SLNRRRSFDKPPPT PRL KPFRSPGPR+R  HVPVRSSSFG+KP+ T  +   S K
Sbjct: 121 GRPSLNRRRSFDKPPPTVPRLGKPFRSPGPRNRTPHVPVRSSSFGAKPSTTTTTIGHSTK 180

Query: 181 PGHLERSSSSKTSPKVGGKPQQPVQNLRSGSNAIAKKSLRRESSNA-------------G 240
           PG LERSSS   S KVGGKPQ P+Q+L++ S+ + KKSLRRESSNA              
Sbjct: 181 PGFLERSSSK--SSKVGGKPQPPIQSLKTSSSYV-KKSLRRESSNAAASASASASASASA 240

Query: 241 SALAEIVPKAKNIAVEQAVHSPSFVLGVNEEELKKIECELDPYLPDPMPELDKELRTDHV 300
           SA AEIVPK+KN AVE    SPSFVLGVNEE+LKKIECELDPYLPDPMPELDK++R D V
Sbjct: 241 SASAEIVPKSKN-AVEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQIRLDQV 300

Query: 301 EKKVDVLDKEEADAKHEAEKQPIQDTEISKADTETPSPIEVEAKLLIQEESNRERTEGVG 360
           EKK  VLDK+E D   E  K  + + E+ +   ETP  +E E  L  QE++ RE  E   
Sbjct: 301 EKKA-VLDKDETDT--EILKVVVTNKEVEE-QKETPRTVETEISLANQEQNEREGEEPKE 360

Query: 361 EEEVK--DEEKPEIITQEKEVD--KDEEKSEISPTQEGIVVLDQKEVEDGENCKEEAE-- 420
           E+  K  + EK E++  E+ ++   D+EKSE               V DGE+CKEE++  
Sbjct: 361 EDNPKRIEVEKEEVVALEESINNKNDQEKSETD------------SVLDGESCKEESDQN 420

Query: 421 ATEKVA-AAEKEGEAVAEKLAVMGAKQRQGS-GRKESPAMYNDVIEETASKLLEKRQNKV 468
           +T K A   EKE E+  +      AK RQG  GRKESPAMYN VIEETASKLLEKR+NKV
Sbjct: 421 STPKAAETTEKEAESTEKT-----AKPRQGGPGRKESPAMYNHVIEETASKLLEKRKNKV 462

[Close]

BLAST of MS001253 vs. TAIR 10
Match: AT5G24880.1 (BEST Arabidopsis thaliana protein match is: calmodulin-binding protein-related (TAIR:AT5G10660.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 79.0 bits (193), Expect = 1.2e-14
Identity = 153/489 (31.29%), Postives = 230/489 (47.03%), Query Frame = 0


Query: 30  TPKSSPRSQQTDHKLAYSSSFSSSSSSSSSSTSSKLTSSSSSPSLSMLSEKHVPNYLKST 89
           T  S+ +++  +  L+ + S         S+ SS+  SS   P      EK +PNYLK T
Sbjct: 3   TTSSTTKAKPDNQNLSRTKSLGRKPKQPLSTASSEDGSSDQKP------EKPLPNYLKPT 62

Query: 90  ASSRHDHGSFKTVKKFVAPAPIPEGKASLNRRRSFDKPPPTAPRLDKPFRSPGPRSRATH 149
            SSR D G  K +KK        +    L RRRSFD+PP +   L  P  S  PR + + 
Sbjct: 63  ISSRPDAG--KLLKKNNNSL---DDNQKLLRRRSFDRPPSS---LTSPSTSASPRIQKS- 122

Query: 150 VPVRSSSFGSKPAPTFPSSAKPGHLERSSS---SKTSPKVGGKPQQ-PVQNLRSGSNAIA 209
             +  S   S+  P  P   KP    RSSS   S+  PK G   +  PV   +SG N+ +
Sbjct: 123 --LNVSPSRSRDRPAVPRE-KPVTALRSSSFHGSRNVPKGGNTAKSPPVAPKKSGLNSSS 182

Query: 210 KKSLRRESSNAGSALAEIVPKA-KNIAVEQAVHSPSFVLGVNEEELKKIECELDPYLPDP 269
             S   +S   GS    I   + K IA++ A  S S     ++EE+ K+E          
Sbjct: 183 TSS---KSKKEGSENVRIKKASDKEIALDSA--SMSSAQEDHQEEILKVE---------- 242

Query: 270 MPELDKELRTDHVEKKVDVLDKEEADAKHEAEKQPIQDTEI-------SKADTETPSPI- 329
                    +DH++    V D +  + K+E E++ +Q+  +        KA++  P+P+ 
Sbjct: 243 ---------SDHLQ----VSDHDIEEPKYEKEEKEVQEKVVQANESVEEKAESSGPTPVA 302

Query: 330 ------------EVEAKLLIQEESNRERTEGVGEEE----------------VKDEEKPE 389
                       E+E KL+  E+   E+TE + E++                + + E PE
Sbjct: 303 SPVGKDCNAVVAELEEKLIKNEDDIEEKTEEMKEQDNNQANKSEEEEDVKKKIDENETPE 362

Query: 390 -IITQEKEVDKDEE---KSEISPTQEGIVVLDQKE------VEDGENCKEEAEATEKVAA 449
            + T+ KEV+  EE   + E    +EG   ++++E       ED +  K E E  EKV  
Sbjct: 363 KVDTESKEVESVEETTQEKEEEVKEEGKERVEEEEKEKEKVKEDDQKEKVEEEEKEKV-K 422

Query: 450 AEKEGEAVAEKLAVMGAKQRQGSGRKESPAMYNDVIEETASKLLEK-RQNKVKALAGAFQ 467
            ++E E V E+ +  G K+    G+KESP+ YNDVI   ASK+ E  R+NKV ALAGAFQ
Sbjct: 423 GDEEKEKVKEEESAEGKKKEVVKGKKESPSAYNDVI---ASKMQENPRKNKVLALAGAFQ 441

[Close]

BLAST of MS001253 vs. TAIR 10
Match: AT5G10660.1 (calmodulin-binding protein-related )

HSP 1 Score: 57.8 bits (138), Expect = 2.8e-08
Identity = 136/449 (30.29%), Postives = 192/449 (42.76%), Query Frame = 0


Query: 30  TPKSSPRSQQTDHKLAYSSSFSSSSSSSSSSTSSKLTSSSSSPSLSMLSEKHVPNYLKST 89
           T  S+ ++++ +  L  S S        SSS        S   ++     K +PNYLK T
Sbjct: 3   TTSSTTKTKRDNKNLTRSKSLGRKPKPVSSSEPEANADGSDRKTIG----KPLPNYLKPT 62

Query: 90  ASSRHDHGSFKTVKKFVAPAPIPEGKASLNRRRSFDKPPP--TAPRLDKPFRSPGPRSRA 149
            SSR D        KF+      E    L RRRSFD PP   T+P      RS       
Sbjct: 63  ISSRPD------PVKFLRKNNAVEENQKLLRRRSFDHPPSSLTSPSTSSAHRSLNTSPAH 122

Query: 150 THVPVRSSSFGSKPAPTFPSSAKPGHLE---RSSSSKTSPKVGGKPQQPVQNLRSGSNAI 209
            H+  + +    KP     S++  G      R SS+  SP V  +    V+      N+ 
Sbjct: 123 PHLRDKPAVPREKPVTGLRSTSFHGSSRGGLRGSSTVKSPPVASRGSSGVKKSGLSGNS- 182

Query: 210 AKKSLRRESSNAGSALAEIVPKAKNIAVEQAVHSPSFVLGVNEEELKKIECELDPYLPDP 269
           + KS +  S N        VPK  +        SP      +EEE+ K+E ++       
Sbjct: 183 SSKSKKEGSGN--------VPKKSSGKEISPDSSPLASAHEDEEEIVKVETDVH------ 242

Query: 270 MPELDKELRTDHVEKKVDVLDKEEADAKHEAEKQPIQDTEISKADTETPSPIEVEAKLLI 329
                    +DH E+      KEE   +     +  ++ E S     T    E +   LI
Sbjct: 243 --------ISDHGEE-----PKEEDKDQFAQPDESGEEKETSPVAAST----EEQKGELI 302

Query: 330 QEESNRERTEGVGEEEVKDEEKPEIITQEKEVDKDEEKSEISPT----QEGIVVLDQKEV 389
            E+ + E+ E   E E  +E   E   + K+   DEE SE   T     E + V + KE 
Sbjct: 303 DEDKSTEQIEEPKEPENIEENNSEEEEEVKKKSDDEENSETVATTTDMNEAVNVEESKE- 362

Query: 390 EDGENCKEEAEATEKVAAAEKEGEAVA--EKLAVMGAKQRQGSGRKESPAMYNDVIEETA 449
           E+ E  + + E  E  AA E+  E +A  E+L   G K     G+KESP  YNDVI   A
Sbjct: 363 EEKEEAEVKEEEGESSAAKEETTETMAQVEELPEEGTKNEVVQGKKESPTAYNDVI---A 405

Query: 450 SKLLE-KRQNKVKALAGAFQTVIDYESSS 467
           SK+ E  ++NKV ALAGAFQTVIDYE+++
Sbjct: 423 SKMQENSKKNKVLALAGAFQTVIDYETAA 405

The following BLAST results are available for this feature:

BLAST of MS001253 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022131479.1	1.8e-227	89.38	uncharacterized protein DDB_G0271670 isoform X2 [Momordica charantia]	[more]
XP_022131477.1	1.6e-223	83.88	uncharacterized protein DDB_G0271670 isoform X1 [Momordica charantia] >XP_022131...	[more]
KAG7029749.1	1.9e-112	60.71	hypothetical protein SDJN02_08091, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023546370.1	3.3e-112	60.12	cell wall protein RBR3-like [Cucurbita pepo subsp. pepo]	[more]
KAG6598809.1	5.6e-112	60.52	hypothetical protein SDJN03_08587, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of MS001253 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS001253 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BQC8	8.7e-228	89.38	uncharacterized protein DDB_G0271670 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1BTH3	7.6e-224	83.88	uncharacterized protein DDB_G0271670 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1HE16	6.7e-111	59.92	neurofilament medium polypeptide-like OS=Cucurbita moschata OX=3662 GN=LOC111462...	[more]
A0A6J1K599	1.5e-110	59.92	microtubule-associated protein RP/EB family member 1-like OS=Cucurbita maxima OX...	[more]
A0A0A0LS85	1.1e-102	57.23	CaM_binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G378000...	[more]

BLAST of MS001253 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G24880.1	1.2e-14	31.29	BEST Arabidopsis thaliana protein match is: calmodulin-binding protein-related (...	[more]
AT5G10660.1	2.8e-08	30.29	calmodulin-binding protein-related	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012417	Calmodulin-binding domain, plant	SMART	SM01054	CaM_binding_2	coord: 344..461 e-value: 8.0E-4 score: 18.9
IPR012417	Calmodulin-binding domain, plant	PFAM	PF07839	CaM_binding	coord: 354..460 e-value: 6.5E-15 score: 55.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 287..304
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 323..366
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..217
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 149..163
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 17..92
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 171..215
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 287..371
None	No IPR available	PANTHER	PTHR33349	EMB\|CAB62594.1	coord: 53..464
None	No IPR available	PANTHER	PTHR33349:SF20	CALMODULIN-BINDING PROTEIN-LIKE PROTEIN	coord: 53..464

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS001253.1	MS001253.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005516	calmodulin binding