MS001242 (gene) Bitter gourd (TR) v1

Overview


Name	MS001242
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Gamma-glutamylcyclotransferase
Location	scaffold36: 2595773 .. 2597181 (-)
RNA-Seq Expression	MS001242
Synteny	MS001242

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCGATTCCGTCATGGTTCTTTGGGTTTTTGGCTACGGTTCTCTGGTGTGGAATCCGGGATTTGACTTCGATGAGAAAGTGATTGGTTTCATTAGAGATTATAGGCGCGTGTTTGACCTTGGTAAGTTTCTTCATCAACATTTGAAGTTTCAACATTCATTATTGTCAACAAAAGTTTTCTAATATCCCATTTCCCGTTCTACAGCTTGTATTGACCACAGAGGTACACCTGAACACCCTGCTAGAACTCTCACGCTGGAACCCAAAGAAGGATCCATTTGCGTAAGATTTCATCCATAATTACCAGTATCCATTCATGAGTTTATTCATTGTTTTCAATTTCCGTGATGCCAAAATTTTTGTGGCCTTGCCTGATTAGTATTTTTTTTTTCTTCTTTCTGTTAGTGGGGTGCTGCATTTTGCATCCGTGGAGGTCCTGAAAGAGAGAGAGTGGCCATGGAGGTATATCCCTCAATTTTTACTTTAATTTCACTCAAACCCAGAATTTATTCCCTAACCCTTCTTTGTCTTTTGCAATGTATAGTATCTGGAGAGAAGAGAGTGTGAATATGACCAAAAGACTATTGTAAACTTTTACAAGGTAAGGCTAAGGCCACGAGAGAATTTTCTTCATTATTAACTGAGTTTCTATTGATAACTTCTTGAATTATCTTCTTATTTCCTTTCTATTTTCTTTCTTGATTCAGGACGAAAACTCCATGGAACCCACATTGACAGGAGTTTTAGTGTAAGCCTTTTTCTTTAACACAATCATTTTTTAATCTTTGTTTGGGACAAGCTGAACAAGAACATCTCTGTTTTCCTGATCTGATAAATTTCACATTTATGTATGCAGTTTCACATCCACGTTTGATAAATCACTAAACAAGTACTACTTAGGACCAGCTCCCTTGGAGGATATGGCTAGGTAAATGGCAGAAGCTTAGACCATCTGTTCTTTTAACACTGTTCTGGCAATGGTCTACTTCCCCTTTAAATGGGTTCCTTCTAAATCGCTCATTTACTAATTTTTCAGCCAAATTGCTACTGCATCTGGCCCATGTGGGAACAATAGAGACTATCTATTCCGTTTAGAGAAGGCCTTGTTCGATATCGGTAACGATCCAATTCCTAGACTAAAATTGCTTTCTGATGTGATGATTGGTATGAACAACATTTGAGTCAATACTTACTTTATGTATCTCTGATCAGGCCACGAAGACAATATGGTGATCGAGCTAGCGAACGAAGTGAGGAAAGTCCTCGGGATGGTGGTTGTAAAGGGGATCATTATGAAGGATACAAAGAAGAAGGCAGTTGGACCGACAGCGGCACACCTGCCTTTCATGTCCTTCATCTCAGGAATTCAGGTGCATCCGCTAGCAGAAACCGCGGTGGCCATGGATTCT

mRNA sequence

CTCGATTCCGTCATGGTTCTTTGGGTTTTTGGCTACGGTTCTCTGGTGTGGAATCCGGGATTTGACTTCGATGAGAAAGTGATTGGTTTCATTAGAGATTATAGGCGCGTGTTTGACCTTGCTTGTATTGACCACAGAGGTACACCTGAACACCCTGCTAGAACTCTCACGCTGGAACCCAAAGAAGGATCCATTTGCTGGGGTGCTGCATTTTGCATCCGTGGAGGTCCTGAAAGAGAGAGAGTGGCCATGGAGTATCTGGAGAGAAGAGAGTGTGAATATGACCAAAAGACTATTGTAAACTTTTACAAGGACGAAAACTCCATGGAACCCACATTGACAGGAGTTTTAGTTTTCACATCCACGTTTGATAAATCACTAAACAAGTACTACTTAGGACCAGCTCCCTTGGAGGATATGGCTAGCCAAATTGCTACTGCATCTGGCCCATGTGGGAACAATAGAGACTATCTATTCCGTTTAGAGAAGGCCTTGTTCGATATCGGCCACGAAGACAATATGGTGATCGAGCTAGCGAACGAAGTGAGGAAAGTCCTCGGGATGGTGGTTGTAAAGGGGATCATTATGAAGGATACAAAGAAGAAGGCAGTTGGACCGACAGCGGCACACCTGCCTTTCATGTCCTTCATCTCAGGAATTCAGGTGCATCCGCTAGCAGAAACCGCGGTGGCCATGGATTCT

Coding sequence (CDS)

CTCGATTCCGTCATGGTTCTTTGGGTTTTTGGCTACGGTTCTCTGGTGTGGAATCCGGGATTTGACTTCGATGAGAAAGTGATTGGTTTCATTAGAGATTATAGGCGCGTGTTTGACCTTGCTTGTATTGACCACAGAGGTACACCTGAACACCCTGCTAGAACTCTCACGCTGGAACCCAAAGAAGGATCCATTTGCTGGGGTGCTGCATTTTGCATCCGTGGAGGTCCTGAAAGAGAGAGAGTGGCCATGGAGTATCTGGAGAGAAGAGAGTGTGAATATGACCAAAAGACTATTGTAAACTTTTACAAGGACGAAAACTCCATGGAACCCACATTGACAGGAGTTTTAGTTTTCACATCCACGTTTGATAAATCACTAAACAAGTACTACTTAGGACCAGCTCCCTTGGAGGATATGGCTAGCCAAATTGCTACTGCATCTGGCCCATGTGGGAACAATAGAGACTATCTATTCCGTTTAGAGAAGGCCTTGTTCGATATCGGCCACGAAGACAATATGGTGATCGAGCTAGCGAACGAAGTGAGGAAAGTCCTCGGGATGGTGGTTGTAAAGGGGATCATTATGAAGGATACAAAGAAGAAGGCAGTTGGACCGACAGCGGCACACCTGCCTTTCATGTCCTTCATCTCAGGAATTCAGGTGCATCCGCTAGCAGAAACCGCGGTGGCCATGGATTCT

Protein sequence

LDSVMVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPEHPARTLTLEPKEGSICWGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFTSTFDKSLNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHEDNMVIELANEVRKVLGMVVVKGIIMKDTKKKAVGPTAAHLPFMSFISGIQVHPLAETAVAMDS

Homology

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BLAST of MS001242 vs. NCBI nr
Match: XP_022132329.1 (gamma-glutamylcyclotransferase 2-1-like [Momordica charantia])

HSP 1 Score: 468.8 bits (1205), Expect = 2.8e-128
Identity = 225/230 (97.83%), Postives = 228/230 (99.13%), Query Frame = 0


Query: 5   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPEHPARTLTLEPKEGS 64
           MVLWVFGYGSLVWNPGFDFDEKVIGFIRDY+RVFDLACIDHRGTPEHPARTLTLEPKEGS
Sbjct: 1   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYKRVFDLACIDHRGTPEHPARTLTLEPKEGS 60

Query: 65  ICWGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFTSTFD 124
           ICWGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFTSTFD
Sbjct: 61  ICWGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFTSTFD 120

Query: 125 KSLNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHEDNMVIELANEVRK 184
           KSLNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHED+MVIELANEVRK
Sbjct: 121 KSLNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHEDDMVIELANEVRK 180

Query: 185 VLGMVVVKGIIMKDTKKKAVGPTAAHLPFMSFISGIQVHPLAETAVAMDS 235
           VLGMVV KG+IMKDTKKKAVGP AAHLPFMSFISGIQVHPLAETAVAMDS
Sbjct: 181 VLGMVVGKGMIMKDTKKKAVGPMAAHLPFMSFISGIQVHPLAETAVAMDS 230

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BLAST of MS001242 vs. NCBI nr
Match: XP_004138775.3 (gamma-glutamylcyclotransferase 2-1 [Cucumis sativus] >KAE8652174.1 hypothetical protein Csa_021981 [Cucumis sativus])

HSP 1 Score: 430.3 bits (1105), Expect = 1.1e-116
Identity = 203/234 (86.75%), Postives = 219/234 (93.59%), Query Frame = 0


Query: 1   LDSVMVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPEHPARTLTLEP 60
           ++S+MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPE+PARTLTLEP
Sbjct: 99  INSIMVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPENPARTLTLEP 158

Query: 61  KEGSICWGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFT 120
           KEGSICWGAAFCIRGGPERER AMEYLE+RECEYDQKTIV+FYKDE+SMEPTLTGVLVFT
Sbjct: 159 KEGSICWGAAFCIRGGPEREREAMEYLEKRECEYDQKTIVDFYKDEDSMEPTLTGVLVFT 218

Query: 121 STFDKSLNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHEDNMVIELAN 180
           STFDK LNKYYLGPAPLE MASQIATASGPCGNNRDYLFRLEKALFDIGHED+MVIELAN
Sbjct: 219 STFDKVLNKYYLGPAPLEQMASQIATASGPCGNNRDYLFRLEKALFDIGHEDDMVIELAN 278

Query: 181 EVRKVLGMVVVKGIIMKDTKKKAVGPTAAHLPFMSFISGIQVHPLAETAVAMDS 235
           EVR +LG V   G+++KD KKK +GPT  HLPFMSFISG+QVHP+ +  VAMDS
Sbjct: 279 EVRIILGKVGKVGMVLKD-KKKPIGPTTTHLPFMSFISGVQVHPMVDATVAMDS 331

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BLAST of MS001242 vs. NCBI nr
Match: XP_008445065.1 (PREDICTED: putative glutathione-specific gamma-glutamylcyclotransferase 2 [Cucumis melo])

HSP 1 Score: 422.2 bits (1084), Expect = 3.0e-114
Identity = 204/231 (88.31%), Postives = 216/231 (93.51%), Query Frame = 0


Query: 5   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPEHPARTLTLEPKEGS 64
           MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPE+PARTLTLEPKEGS
Sbjct: 1   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPENPARTLTLEPKEGS 60

Query: 65  ICWGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFTSTFD 124
           ICWGAAFCIRGGPERER AMEYLE+RECEYDQKTIV FYKDE+SMEPTLTGVLVFTSTFD
Sbjct: 61  ICWGAAFCIRGGPEREREAMEYLEKRECEYDQKTIVEFYKDEDSMEPTLTGVLVFTSTFD 120

Query: 125 KSLNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHEDNMVIELANEVRK 184
           K LNKYYLGPAPLE MASQIATASGPCGNNRDYLFRLEKALFDIGHED+MVIELANEVR 
Sbjct: 121 KVLNKYYLGPAPLEQMASQIATASGPCGNNRDYLFRLEKALFDIGHEDDMVIELANEVRN 180

Query: 185 VLGMVVVKGIIMKDTKKKAVGP-TAAHLPFMSFISGIQVHPLAETAVAMDS 235
           +LG  V KG+++K+ KKK +GP T  HLPFMSFISGIQVHP+A+  VAMDS
Sbjct: 181 ILGK-VGKGVVLKE-KKKPIGPTTTTHLPFMSFISGIQVHPMADATVAMDS 229

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BLAST of MS001242 vs. NCBI nr
Match: KAA0065011.1 (putative glutathione-specific gamma-glutamylcyclotransferase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 420.6 bits (1080), Expect = 8.7e-114
Identity = 203/231 (87.88%), Postives = 215/231 (93.07%), Query Frame = 0


Query: 5   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPEHPARTLTLEPKEGS 64
           MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPE+PARTLTLEPKEGS
Sbjct: 1   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPENPARTLTLEPKEGS 60

Query: 65  ICWGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFTSTFD 124
           ICWGAAFCIRGGPERER AMEYLE+RECEYDQKTIV FYKDE+SMEPTLTGVLVFTSTFD
Sbjct: 61  ICWGAAFCIRGGPEREREAMEYLEKRECEYDQKTIVEFYKDEDSMEPTLTGVLVFTSTFD 120

Query: 125 KSLNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHEDNMVIELANEVRK 184
           K LNKYYLGPAPLE MASQIATASGPCGNNRDYLFRLEKALFDIGHED+MVIELANEVR 
Sbjct: 121 KVLNKYYLGPAPLEQMASQIATASGPCGNNRDYLFRLEKALFDIGHEDDMVIELANEVRN 180

Query: 185 VLGMVVVKGIIMKDTKKKAVGP-TAAHLPFMSFISGIQVHPLAETAVAMDS 235
           +LG  V KG+++K+ KKK +GP T  HLPFMSFISGIQVHP+ +  VAMDS
Sbjct: 181 ILGK-VGKGVVLKE-KKKPIGPTTTTHLPFMSFISGIQVHPMTDATVAMDS 229

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BLAST of MS001242 vs. NCBI nr
Match: XP_038884302.1 (gamma-glutamylcyclotransferase 2-1-like [Benincasa hispida])

HSP 1 Score: 410.2 bits (1053), Expect = 1.2e-110
Identity = 198/230 (86.09%), Postives = 213/230 (92.61%), Query Frame = 0


Query: 5   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPEHPARTLTLEPKEGS 64
           MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPEHPARTLTLEPK+GS
Sbjct: 1   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPEHPARTLTLEPKQGS 60

Query: 65  ICWGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFTSTFD 124
           ICWGAAFCIRG PERER AMEYLE+RECEYDQKTIV+FY DE+S EPTLTGV+VFTSTFD
Sbjct: 61  ICWGAAFCIRGCPERERAAMEYLEKRECEYDQKTIVDFYTDEDSFEPTLTGVVVFTSTFD 120

Query: 125 KSLNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHEDNMVIELANEVRK 184
           K +NKYYLGPAPLE MASQIATASGPCGNNRDYLF LEKALF+IGHED+MVIELANEVR 
Sbjct: 121 KVINKYYLGPAPLEQMASQIATASGPCGNNRDYLFLLEKALFEIGHEDDMVIELANEVRN 180

Query: 185 VLGMVVVKGIIMKDTKKKAVGPTAAHLPFMSFISGIQVHPLAETAVAMDS 235
           +LG  V KG+I+K+ KKK +G T  HLPFMSFISG+QVHPLAE A+AMDS
Sbjct: 181 ILGK-VGKGVILKE-KKKPIGST-THLPFMSFISGVQVHPLAEAAMAMDS 227

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BLAST of MS001242 vs. ExPASy Swiss-Prot
Match: Q8GY54 (Gamma-glutamylcyclotransferase 2-1 OS=Arabidopsis thaliana OX=3702 GN=GGCT2;1 PE=1 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 1.8e-85
Identity = 141/184 (76.63%), Postives = 164/184 (89.13%), Query Frame = 0


Query: 5   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPEHPARTLTLEPKEGS 64
           MVLWVFGYGSL+WNPGFDFDEK+IG+I+DY+RVFDLACIDHRGTPEHPART TLE   G+
Sbjct: 1   MVLWVFGYGSLIWNPGFDFDEKLIGYIKDYKRVFDLACIDHRGTPEHPARTCTLEQSTGA 60

Query: 65  ICWGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFTSTFD 124
           ICWGAA+C+RGGPE+E++AMEYLERRECEYD KT+V FY + ++  P +TGV+VFTST D
Sbjct: 61  ICWGAAYCVRGGPEKEKLAMEYLERRECEYDSKTLVEFYTENDTSTPIVTGVIVFTSTPD 120

Query: 125 KSLNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHEDNMVIELANEVRK 184
           K  NKYYLGPAPLE+MA QIATASGPCGNNR+YLF+LEKA+FDI HE+  VIELANEVRK
Sbjct: 121 KVSNKYYLGPAPLEEMARQIATASGPCGNNREYLFKLEKAMFDIEHEEEYVIELANEVRK 180

Query: 185 VLGM 189
            L +
Sbjct: 181 QLDL 184

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BLAST of MS001242 vs. ExPASy Swiss-Prot
Match: Q84MC1 (Gamma-glutamylcyclotransferase 2-2 OS=Arabidopsis thaliana OX=3702 GN=GGCT2;2 PE=1 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 1.8e-85
Identity = 143/182 (78.57%), Postives = 164/182 (90.11%), Query Frame = 0


Query: 5   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPEHPARTLTLEPKEGS 64
           MV+WVFGYGSLVWNPGF +DEKV+GFI+ Y+RVFDLACIDHRGTPEHPART TLE  E +
Sbjct: 1   MVMWVFGYGSLVWNPGFHYDEKVLGFIKGYKRVFDLACIDHRGTPEHPARTCTLEKAEEA 60

Query: 65  ICWGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFTSTFD 124
           ICWG AFC+RGGPE+ER+AMEYLERRECEYD KT V+FYK+++ ++P +TGV+VFTST D
Sbjct: 61  ICWGTAFCVRGGPEKERLAMEYLERRECEYDLKTSVDFYKEDDPLKPAVTGVIVFTSTPD 120

Query: 125 KSLNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHEDNMVIELANEVRK 184
           K  NKYYLGPAPLEDMA QIATA+GPCGNNRDYLF LEKA+ DIGHE++ VIELANEVRK
Sbjct: 121 KVSNKYYLGPAPLEDMARQIATANGPCGNNRDYLFLLEKAMHDIGHEEDYVIELANEVRK 180

Query: 185 VL 187
           VL
Sbjct: 181 VL 182

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BLAST of MS001242 vs. ExPASy Swiss-Prot
Match: Q84QC1 (Gamma-glutamylcyclotransferase 2-3 OS=Arabidopsis thaliana OX=3702 GN=GGCT2;3 PE=1 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 1.4e-50
Identity = 96/182 (52.75%), Postives = 124/182 (68.13%), Query Frame = 0


Query: 5   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPEHPARTLTLEPKEGS 64
           M +WVFGYGSL+W  GF FDE + GFI+ YRRVF     DHRGTP+ P RT+TLE     
Sbjct: 1   MAMWVFGYGSLIWKTGFPFDESLPGFIKGYRRVFHQGSTDHRGTPDFPGRTVTLEAAHEE 60

Query: 65  ICWGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFTSTFD 124
           +C G A+ I    E +R A+ +LE RE +YDQK  ++F+ D N+ EP + GV+V+ ++ D
Sbjct: 61  VCCGVAYKIT-KEEDKRDALLHLEVREKQYDQKEYLDFFTDSNASEPAVAGVMVYIASPD 120

Query: 125 KSLNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHEDNMVIELANEVRK 184
           K  N  YLGPAPLED+A QI  A GP G NRDYLF LE+AL  +G +D  V +LAN+VR 
Sbjct: 121 KKSNNNYLGPAPLEDIAKQIVKAKGPSGPNRDYLFNLEEALAQLGFKDKHVTDLANQVRH 180

Query: 185 VL 187
           +L
Sbjct: 181 IL 181

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BLAST of MS001242 vs. ExPASy Swiss-Prot
Match: Q641Z5 (Putative glutathione-specific gamma-glutamylcyclotransferase 2 OS=Rattus norvegicus OX=10116 GN=Chac2 PE=2 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 2.4e-29
Identity = 69/166 (41.57%), Postives = 94/166 (56.63%), Query Frame = 0


Query: 7   LWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPEHPARTLTLEPKEGSIC 66
           +WVFGYGSL+W   F + +K++G+I +Y R F     DHRG P  P R +TL    G   
Sbjct: 1   MWVFGYGSLIWKVDFPYQDKLVGYITNYSRRFWQGSTDHRGVPGKPGRVVTLVEDPGGSV 60

Query: 67  WGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFTSTFDKS 126
           WG A+ +  G E E     YL+ RE    + T V FY  +++ +P    VL++  T D  
Sbjct: 61  WGVAYKLPVGKEEE--VKTYLDFREKGGYRTTTVIFYPKDSTTKP--FSVLLYIGTCD-- 120

Query: 127 LNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHED 173
            N  YLGPAPLED+A QI  A+GP G N +YLF L  ++  +  ED
Sbjct: 121 -NPNYLGPAPLEDIAEQIFNAAGPSGRNTEYLFELADSIRKLVPED 159

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BLAST of MS001242 vs. ExPASy Swiss-Prot
Match: Q9CQG1 (Putative glutathione-specific gamma-glutamylcyclotransferase 2 OS=Mus musculus OX=10090 GN=Chac2 PE=1 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 3.1e-29
Identity = 69/166 (41.57%), Postives = 94/166 (56.63%), Query Frame = 0


Query: 7   LWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPEHPARTLTLEPKEGSIC 66
           +WVFGYGSL+W   F + +K++G+I +Y R F     DHRG P  P R +TL    G   
Sbjct: 1   MWVFGYGSLIWKVDFPYQDKLVGYITNYSRRFWQGSTDHRGVPGKPGRVVTLVEDPGGSV 60

Query: 67  WGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFTSTFDKS 126
           WG A+ +  G E E     YL+ RE    + T V FY  +++ +P    VL++  T D  
Sbjct: 61  WGVAYKLPVGKEEE--VKTYLDFREKGGYRTTTVIFYPKDSTTKP--FSVLLYIGTCD-- 120

Query: 127 LNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHED 173
            N  YLGPAPLED+A QI  A+GP G N +YLF L  ++  +  ED
Sbjct: 121 -NPNYLGPAPLEDIAEQIFNAAGPSGRNTEYLFELADSVRKLVPED 159

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BLAST of MS001242 vs. ExPASy TrEMBL
Match: A0A6J1BSS1 (Gamma-glutamylcyclotransferase OS=Momordica charantia OX=3673 GN=LOC111005209 PE=3 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 1.4e-128
Identity = 225/230 (97.83%), Postives = 228/230 (99.13%), Query Frame = 0


Query: 5   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPEHPARTLTLEPKEGS 64
           MVLWVFGYGSLVWNPGFDFDEKVIGFIRDY+RVFDLACIDHRGTPEHPARTLTLEPKEGS
Sbjct: 1   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYKRVFDLACIDHRGTPEHPARTLTLEPKEGS 60

Query: 65  ICWGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFTSTFD 124
           ICWGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFTSTFD
Sbjct: 61  ICWGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFTSTFD 120

Query: 125 KSLNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHEDNMVIELANEVRK 184
           KSLNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHED+MVIELANEVRK
Sbjct: 121 KSLNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHEDDMVIELANEVRK 180

Query: 185 VLGMVVVKGIIMKDTKKKAVGPTAAHLPFMSFISGIQVHPLAETAVAMDS 235
           VLGMVV KG+IMKDTKKKAVGP AAHLPFMSFISGIQVHPLAETAVAMDS
Sbjct: 181 VLGMVVGKGMIMKDTKKKAVGPMAAHLPFMSFISGIQVHPLAETAVAMDS 230

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BLAST of MS001242 vs. ExPASy TrEMBL
Match: A0A0A0LLN4 (Gamma-glutamylcyclotransferase OS=Cucumis sativus OX=3659 GN=Csa_2G377370 PE=3 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 2.4e-117
Identity = 203/234 (86.75%), Postives = 219/234 (93.59%), Query Frame = 0


Query: 1   LDSVMVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPEHPARTLTLEP 60
           ++S+MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPE+PARTLTLEP
Sbjct: 96  INSIMVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPENPARTLTLEP 155

Query: 61  KEGSICWGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFT 120
           KEGSICWGAAFCIRGGPERER AMEYLE+RECEYDQKTIV+FYKDE+SMEPTLTGVLVFT
Sbjct: 156 KEGSICWGAAFCIRGGPEREREAMEYLEKRECEYDQKTIVDFYKDEDSMEPTLTGVLVFT 215

Query: 121 STFDKSLNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHEDNMVIELAN 180
           STFDK LNKYYLGPAPLE MASQIATASGPCGNNRDYLFRLEKALFDIGHED+MVIELAN
Sbjct: 216 STFDKVLNKYYLGPAPLEQMASQIATASGPCGNNRDYLFRLEKALFDIGHEDDMVIELAN 275

Query: 181 EVRKVLGMVVVKGIIMKDTKKKAVGPTAAHLPFMSFISGIQVHPLAETAVAMDS 235
           EVR +LG V   G+++KD KKK +GPT  HLPFMSFISG+QVHP+ +  VAMDS
Sbjct: 276 EVRNILGKVGKVGMVLKD-KKKPIGPTTTHLPFMSFISGVQVHPMVDATVAMDS 328

[Close]

BLAST of MS001242 vs. ExPASy TrEMBL
Match: A0A1S3BBC1 (Gamma-glutamylcyclotransferase OS=Cucumis melo OX=3656 GN=LOC103488216 PE=3 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 1.5e-114
Identity = 204/231 (88.31%), Postives = 216/231 (93.51%), Query Frame = 0


Query: 5   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPEHPARTLTLEPKEGS 64
           MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPE+PARTLTLEPKEGS
Sbjct: 1   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPENPARTLTLEPKEGS 60

Query: 65  ICWGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFTSTFD 124
           ICWGAAFCIRGGPERER AMEYLE+RECEYDQKTIV FYKDE+SMEPTLTGVLVFTSTFD
Sbjct: 61  ICWGAAFCIRGGPEREREAMEYLEKRECEYDQKTIVEFYKDEDSMEPTLTGVLVFTSTFD 120

Query: 125 KSLNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHEDNMVIELANEVRK 184
           K LNKYYLGPAPLE MASQIATASGPCGNNRDYLFRLEKALFDIGHED+MVIELANEVR 
Sbjct: 121 KVLNKYYLGPAPLEQMASQIATASGPCGNNRDYLFRLEKALFDIGHEDDMVIELANEVRN 180

Query: 185 VLGMVVVKGIIMKDTKKKAVGP-TAAHLPFMSFISGIQVHPLAETAVAMDS 235
           +LG  V KG+++K+ KKK +GP T  HLPFMSFISGIQVHP+A+  VAMDS
Sbjct: 181 ILGK-VGKGVVLKE-KKKPIGPTTTTHLPFMSFISGIQVHPMADATVAMDS 229

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BLAST of MS001242 vs. ExPASy TrEMBL
Match: A0A5A7VGZ3 (Gamma-glutamylcyclotransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003350 PE=3 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 4.2e-114
Identity = 203/231 (87.88%), Postives = 215/231 (93.07%), Query Frame = 0


Query: 5   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPEHPARTLTLEPKEGS 64
           MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPE+PARTLTLEPKEGS
Sbjct: 1   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPENPARTLTLEPKEGS 60

Query: 65  ICWGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFTSTFD 124
           ICWGAAFCIRGGPERER AMEYLE+RECEYDQKTIV FYKDE+SMEPTLTGVLVFTSTFD
Sbjct: 61  ICWGAAFCIRGGPEREREAMEYLEKRECEYDQKTIVEFYKDEDSMEPTLTGVLVFTSTFD 120

Query: 125 KSLNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHEDNMVIELANEVRK 184
           K LNKYYLGPAPLE MASQIATASGPCGNNRDYLFRLEKALFDIGHED+MVIELANEVR 
Sbjct: 121 KVLNKYYLGPAPLEQMASQIATASGPCGNNRDYLFRLEKALFDIGHEDDMVIELANEVRN 180

Query: 185 VLGMVVVKGIIMKDTKKKAVGP-TAAHLPFMSFISGIQVHPLAETAVAMDS 235
           +LG  V KG+++K+ KKK +GP T  HLPFMSFISGIQVHP+ +  VAMDS
Sbjct: 181 ILGK-VGKGVVLKE-KKKPIGPTTTTHLPFMSFISGIQVHPMTDATVAMDS 229

[Close]

BLAST of MS001242 vs. ExPASy TrEMBL
Match: A0A6J1K5T1 (Gamma-glutamylcyclotransferase OS=Cucurbita maxima OX=3661 GN=LOC111491933 PE=3 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 6.3e-110
Identity = 197/230 (85.65%), Postives = 209/230 (90.87%), Query Frame = 0


Query: 5   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPEHPARTLTLEPKEGS 64
           MVLWVFGYGSLVWNPGFDFDEK++GFIRDYRRVFDLACIDHRGTPE+PARTLTLEPKEGS
Sbjct: 1   MVLWVFGYGSLVWNPGFDFDEKMMGFIRDYRRVFDLACIDHRGTPENPARTLTLEPKEGS 60

Query: 65  ICWGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFTSTFD 124
           ICWGAA+CIRGGPE+E+ AMEYLERRECEYDQKTIV+FYKDE SMEPTLTGVLVFTST D
Sbjct: 61  ICWGAAYCIRGGPEKEKEAMEYLERRECEYDQKTIVDFYKDEGSMEPTLTGVLVFTSTPD 120

Query: 125 KSLNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHEDNMVIELANEVRK 184
           K LNKYYLGPAPLE MASQIATASGPCGNNRDYLFRLEKALFDIGHE++MVIELANEVR 
Sbjct: 121 KLLNKYYLGPAPLEQMASQIATASGPCGNNRDYLFRLEKALFDIGHEEDMVIELANEVRN 180

Query: 185 VLGMVVVKGIIMKDTKKKAVGPTAAHLPFMSFISGIQVHPLAETAVAMDS 235
           VLG V          KKK +GPT  HLPFMSFISG+QVHPLAE AVA+DS
Sbjct: 181 VLGKV---------GKKKLIGPT-THLPFMSFISGVQVHPLAEAAVAIDS 220

[Close]

BLAST of MS001242 vs. TAIR 10
Match: AT4G31290.1 (ChaC-like family protein )

HSP 1 Score: 317.0 bits (811), Expect = 1.3e-86
Identity = 143/182 (78.57%), Postives = 164/182 (90.11%), Query Frame = 0


Query: 5   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPEHPARTLTLEPKEGS 64
           MV+WVFGYGSLVWNPGF +DEKV+GFI+ Y+RVFDLACIDHRGTPEHPART TLE  E +
Sbjct: 1   MVMWVFGYGSLVWNPGFHYDEKVLGFIKGYKRVFDLACIDHRGTPEHPARTCTLEKAEEA 60

Query: 65  ICWGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFTSTFD 124
           ICWG AFC+RGGPE+ER+AMEYLERRECEYD KT V+FYK+++ ++P +TGV+VFTST D
Sbjct: 61  ICWGTAFCVRGGPEKERLAMEYLERRECEYDLKTSVDFYKEDDPLKPAVTGVIVFTSTPD 120

Query: 125 KSLNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHEDNMVIELANEVRK 184
           K  NKYYLGPAPLEDMA QIATA+GPCGNNRDYLF LEKA+ DIGHE++ VIELANEVRK
Sbjct: 121 KVSNKYYLGPAPLEDMARQIATANGPCGNNRDYLFLLEKAMHDIGHEEDYVIELANEVRK 180

Query: 185 VL 187
           VL
Sbjct: 181 VL 182

[Close]

BLAST of MS001242 vs. TAIR 10
Match: AT5G26220.1 (ChaC-like family protein )

HSP 1 Score: 317.0 bits (811), Expect = 1.3e-86
Identity = 141/184 (76.63%), Postives = 164/184 (89.13%), Query Frame = 0


Query: 5   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPEHPARTLTLEPKEGS 64
           MVLWVFGYGSL+WNPGFDFDEK+IG+I+DY+RVFDLACIDHRGTPEHPART TLE   G+
Sbjct: 1   MVLWVFGYGSLIWNPGFDFDEKLIGYIKDYKRVFDLACIDHRGTPEHPARTCTLEQSTGA 60

Query: 65  ICWGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFTSTFD 124
           ICWGAA+C+RGGPE+E++AMEYLERRECEYD KT+V FY + ++  P +TGV+VFTST D
Sbjct: 61  ICWGAAYCVRGGPEKEKLAMEYLERRECEYDSKTLVEFYTENDTSTPIVTGVIVFTSTPD 120

Query: 125 KSLNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHEDNMVIELANEVRK 184
           K  NKYYLGPAPLE+MA QIATASGPCGNNR+YLF+LEKA+FDI HE+  VIELANEVRK
Sbjct: 121 KVSNKYYLGPAPLEEMARQIATASGPCGNNREYLFKLEKAMFDIEHEEEYVIELANEVRK 180

Query: 185 VLGM 189
            L +
Sbjct: 181 QLDL 184

[Close]

BLAST of MS001242 vs. TAIR 10
Match: AT1G44790.1 (ChaC-like family protein )

HSP 1 Score: 201.1 bits (510), Expect = 1.0e-51
Identity = 96/182 (52.75%), Postives = 124/182 (68.13%), Query Frame = 0


Query: 5   MVLWVFGYGSLVWNPGFDFDEKVIGFIRDYRRVFDLACIDHRGTPEHPARTLTLEPKEGS 64
           M +WVFGYGSL+W  GF FDE + GFI+ YRRVF     DHRGTP+ P RT+TLE     
Sbjct: 1   MAMWVFGYGSLIWKTGFPFDESLPGFIKGYRRVFHQGSTDHRGTPDFPGRTVTLEAAHEE 60

Query: 65  ICWGAAFCIRGGPERERVAMEYLERRECEYDQKTIVNFYKDENSMEPTLTGVLVFTSTFD 124
           +C G A+ I    E +R A+ +LE RE +YDQK  ++F+ D N+ EP + GV+V+ ++ D
Sbjct: 61  VCCGVAYKIT-KEEDKRDALLHLEVREKQYDQKEYLDFFTDSNASEPAVAGVMVYIASPD 120

Query: 125 KSLNKYYLGPAPLEDMASQIATASGPCGNNRDYLFRLEKALFDIGHEDNMVIELANEVRK 184
           K  N  YLGPAPLED+A QI  A GP G NRDYLF LE+AL  +G +D  V +LAN+VR 
Sbjct: 121 KKSNNNYLGPAPLEDIAKQIVKAKGPSGPNRDYLFNLEEALAQLGFKDKHVTDLANQVRH 180

Query: 185 VL 187
           +L
Sbjct: 181 IL 181

The following BLAST results are available for this feature:

BLAST of MS001242 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132329.1	2.8e-128	97.83	gamma-glutamylcyclotransferase 2-1-like [Momordica charantia]	[more]
XP_004138775.3	1.1e-116	86.75	gamma-glutamylcyclotransferase 2-1 [Cucumis sativus] >KAE8652174.1 hypothetical ...	[more]
XP_008445065.1	3.0e-114	88.31	PREDICTED: putative glutathione-specific gamma-glutamylcyclotransferase 2 [Cucum...	[more]
KAA0065011.1	8.7e-114	87.88	putative glutathione-specific gamma-glutamylcyclotransferase 2 [Cucumis melo var...	[more]
XP_038884302.1	1.2e-110	86.09	gamma-glutamylcyclotransferase 2-1-like [Benincasa hispida]	[more]

BLAST of MS001242 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GY54	1.8e-85	76.63	Gamma-glutamylcyclotransferase 2-1 OS=Arabidopsis thaliana OX=3702 GN=GGCT2;1 PE...	[more]
Q84MC1	1.8e-85	78.57	Gamma-glutamylcyclotransferase 2-2 OS=Arabidopsis thaliana OX=3702 GN=GGCT2;2 PE...	[more]
Q84QC1	1.4e-50	52.75	Gamma-glutamylcyclotransferase 2-3 OS=Arabidopsis thaliana OX=3702 GN=GGCT2;3 PE...	[more]
Q641Z5	2.4e-29	41.57	Putative glutathione-specific gamma-glutamylcyclotransferase 2 OS=Rattus norvegi...	[more]
Q9CQG1	3.1e-29	41.57	Putative glutathione-specific gamma-glutamylcyclotransferase 2 OS=Mus musculus O...	[more]

BLAST of MS001242 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BSS1	1.4e-128	97.83	Gamma-glutamylcyclotransferase OS=Momordica charantia OX=3673 GN=LOC111005209 PE...	[more]
A0A0A0LLN4	2.4e-117	86.75	Gamma-glutamylcyclotransferase OS=Cucumis sativus OX=3659 GN=Csa_2G377370 PE=3 S...	[more]
A0A1S3BBC1	1.5e-114	88.31	Gamma-glutamylcyclotransferase OS=Cucumis melo OX=3656 GN=LOC103488216 PE=3 SV=1	[more]
A0A5A7VGZ3	4.2e-114	87.88	Gamma-glutamylcyclotransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s...	[more]
A0A6J1K5T1	6.3e-110	85.65	Gamma-glutamylcyclotransferase OS=Cucurbita maxima OX=3661 GN=LOC111491933 PE=3 ...	[more]

BLAST of MS001242 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31290.1	1.3e-86	78.57	ChaC-like family protein	[more]
AT5G26220.1	1.3e-86	76.63	ChaC-like family protein	[more]
AT1G44790.1	1.0e-51	52.75	ChaC-like family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.490.10		coord: 5..177 e-value: 1.1E-6 score: 30.2
None	No IPR available	PANTHER	PTHR12192:SF23	GAMMA-GLUTAMYLCYCLOTRANSFERASE 2-2	coord: 5..234
IPR006840	Glutathione-specific gamma-glutamylcyclotransferase	PFAM	PF04752	ChaC	coord: 7..184 e-value: 4.8E-46 score: 157.1
IPR006840	Glutathione-specific gamma-glutamylcyclotransferase	PANTHER	PTHR12192	CATION TRANSPORT PROTEIN CHAC-RELATED	coord: 5..234
IPR013024	Gamma-glutamyl cyclotransferase-like	CDD	cd06661	GGCT_like	coord: 9..99 e-value: 7.22685E-8 score: 47.3394

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS001242.1	MS001242.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006751	glutathione catabolic process
molecular_function	GO:0003839	gamma-glutamylcyclotransferase activity