MS001217 (gene) Bitter gourd (TR) v1

BLAST of MS001217 vs. NCBI nr
Match: XP_022131878.1 (protein NRT1/ PTR FAMILY 1.2-like [Momordica charantia])

HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 574/576 (99.65%), Postives = 574/576 (99.65%), Query Frame = 0

Query: 1   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 60
           MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG
Sbjct: 1   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 60

Query: 61  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 120
           MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ
Sbjct: 61  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 120

Query: 121 ARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLKSSGTLKNYF 180
           ARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLKSSGTLKNYF
Sbjct: 121 ARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLKSSGTLKNYF 180

Query: 181 SSVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSS 240
           S VYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSS
Sbjct: 181 SLVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSS 240

Query: 241 LCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNSEEEL 300
           LCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNSEEEL
Sbjct: 241 LCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNSEEEL 300

Query: 301 TSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDRHLTPT 360
           TSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDRHLTPT
Sbjct: 301 TSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDRHLTPT 360

Query: 361 FEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCAFSLVA 420
           FEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFC FSLVA
Sbjct: 361 FEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCTFSLVA 420

Query: 421 TAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPR 480
           TAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPR
Sbjct: 421 TAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPR 480

Query: 481 AMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWLLFAL 540
           AMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWLLFAL
Sbjct: 481 AMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWLLFAL 540

Query: 541 VFANFLYFLVCSKSYGPSKEEASDESNAEDENNILH 577
           VFANFLYFLVCSKSYGPSKEEASDESNAEDENNILH
Sbjct: 541 VFANFLYFLVCSKSYGPSKEEASDESNAEDENNILH 576

BLAST of MS001217 vs. NCBI nr
Match: XP_038885652.1 (protein NRT1/ PTR FAMILY 1.2 isoform X4 [Benincasa hispida])

HSP 1 Score: 859.0 bits (2218), Expect = 2.4e-245
Identity = 438/577 (75.91%), Postives = 490/577 (84.92%), Query Frame = 0

Query: 1   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 60
           ME PS AD+DRDMEEPLL ++D KGGLRTLPFIIAN ALEK+ASQGL+PSMILYLT VYG
Sbjct: 1   MEGPSVADDDRDMEEPLLSTADHKGGLRTLPFIIANGALEKLASQGLSPSMILYLTMVYG 60

Query: 61  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 120
           M+SA AS +IFLWSAA+NFTPIICAFLADSY GRFP+IAAGS+ SFLGMFVLWLTA IPQ
Sbjct: 61  MKSAYASNIIFLWSAATNFTPIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIPQ 120

Query: 121 ARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLK----SSGTL 180
           ARPFCDE+SG C+APS  QL LLYSSYA+M+IGSGCLQSS +AFGADQL +    +SG L
Sbjct: 121 ARPFCDEISGRCDAPSRAQLSLLYSSYAIMSIGSGCLQSSYLAFGADQLYRTSKSNSGIL 180

Query: 181 KNYFSSVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSM 240
            +YF+  Y++ + G LVGMS +VYIQD +GW MGFGVPVA ML S I F SASPLY+KSM
Sbjct: 181 DSYFNLCYISSAVGSLVGMSGMVYIQDRMGWGMGFGVPVALMLLSMITFFSASPLYLKSM 240

Query: 241 PSSSLCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNS 300
           PS S C G+VQVVVA+ KKR M+VPS    E YHH NGSP  +PSDKLRF NKACII NS
Sbjct: 241 PSRSWCAGIVQVVVAACKKRHMQVPSVGIPETYHHENGSPCALPSDKLRFFNKACIIRNS 300

Query: 301 EEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDRH 360
           EEELTSD RASNPW+LCTV+QVE LKAL+RI+PLWSTGILV+  ++QSFY LQ+ASMDRH
Sbjct: 301 EEELTSDRRASNPWTLCTVEQVEDLKALVRIIPLWSTGILVSAALSQSFYTLQVASMDRH 360

Query: 361 LTPTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCAF 420
           LTP+FEVPAGSFG +L+VSLIIWI LY+R+ILPL SK RGK TRLS KTRMGIGILF   
Sbjct: 361 LTPSFEVPAGSFGAMLVVSLIIWITLYNRVILPLASKCRGKPTRLSGKTRMGIGILFSTL 420

Query: 421 SLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYF 480
           SL  +AIVE+NRRA+AIKEGFSDDP AVV+MSAFWTLPRYI  GM EGF+A+GQIEFFY 
Sbjct: 421 SLAVSAIVESNRRALAIKEGFSDDPNAVVNMSAFWTLPRYILFGMGEGFNAIGQIEFFYN 480

Query: 481 ELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWL 540
           ELP+AMSSVATSL GL MSVGNLAASFIMT VDNFSK    +SWVS+NIN GH DYYYWL
Sbjct: 481 ELPKAMSSVATSLLGLNMSVGNLAASFIMTTVDNFSKAAEVKSWVSSNINKGHSDYYYWL 540

Query: 541 LFALVFANFLYFLVCSKSYGPSKEEASDESNAEDENN 574
           LF L+FANFLYFL CSKSYGPSKEEA   SNAED NN
Sbjct: 541 LFGLLFANFLYFLACSKSYGPSKEEAGGGSNAEDHNN 577

BLAST of MS001217 vs. NCBI nr
Match: XP_038885651.1 (protein NRT1/ PTR FAMILY 1.2 isoform X3 [Benincasa hispida])

HSP 1 Score: 859.0 bits (2218), Expect = 2.4e-245
Identity = 438/577 (75.91%), Postives = 490/577 (84.92%), Query Frame = 0

Query: 1   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 60
           ME PS AD+DRDMEEPLL ++D KGGLRTLPFIIAN ALEK+ASQGL+PSMILYLT VYG
Sbjct: 1   MEGPSVADDDRDMEEPLLSTADHKGGLRTLPFIIANGALEKLASQGLSPSMILYLTMVYG 60

Query: 61  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 120
           M+SA AS +IFLWSAA+NFTPIICAFLADSY GRFP+IAAGS+ SFLGMFVLWLTA IPQ
Sbjct: 61  MKSAYASNIIFLWSAATNFTPIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIPQ 120

Query: 121 ARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLK----SSGTL 180
           ARPFCDE+SG C+APS  QL LLYSSYA+M+IGSGCLQSS +AFGADQL +    +SG L
Sbjct: 121 ARPFCDEISGRCDAPSRAQLSLLYSSYAIMSIGSGCLQSSYLAFGADQLYRTSKSNSGIL 180

Query: 181 KNYFSSVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSM 240
            +YF+  Y++ + G LVGMS +VYIQD +GW MGFGVPVA ML S I F SASPLY+KSM
Sbjct: 181 DSYFNLCYISSAVGSLVGMSGMVYIQDRMGWGMGFGVPVALMLLSMITFFSASPLYLKSM 240

Query: 241 PSSSLCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNS 300
           PS S C G+VQVVVA+ KKR M+VPS    E YHH NGSP  +PSDKLRF NKACII NS
Sbjct: 241 PSRSWCAGIVQVVVAACKKRHMQVPSVGIPETYHHENGSPCALPSDKLRFFNKACIIRNS 300

Query: 301 EEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDRH 360
           EEELTSD RASNPW+LCTV+QVE LKAL+RI+PLWSTGILV+  ++QSFY LQ+ASMDRH
Sbjct: 301 EEELTSDRRASNPWTLCTVEQVEDLKALVRIIPLWSTGILVSAALSQSFYTLQVASMDRH 360

Query: 361 LTPTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCAF 420
           LTP+FEVPAGSFG +L+VSLIIWI LY+R+ILPL SK RGK TRLS KTRMGIGILF   
Sbjct: 361 LTPSFEVPAGSFGAMLVVSLIIWITLYNRVILPLASKCRGKPTRLSGKTRMGIGILFSTL 420

Query: 421 SLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYF 480
           SL  +AIVE+NRRA+AIKEGFSDDP AVV+MSAFWTLPRYI  GM EGF+A+GQIEFFY 
Sbjct: 421 SLAVSAIVESNRRALAIKEGFSDDPNAVVNMSAFWTLPRYILFGMGEGFNAIGQIEFFYN 480

Query: 481 ELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWL 540
           ELP+AMSSVATSL GL MSVGNLAASFIMT VDNFSK    +SWVS+NIN GH DYYYWL
Sbjct: 481 ELPKAMSSVATSLLGLNMSVGNLAASFIMTTVDNFSKAAEVKSWVSSNINKGHSDYYYWL 540

Query: 541 LFALVFANFLYFLVCSKSYGPSKEEASDESNAEDENN 574
           LF L+FANFLYFL CSKSYGPSKEEA   SNAED NN
Sbjct: 541 LFGLLFANFLYFLACSKSYGPSKEEAGGGSNAEDHNN 577

BLAST of MS001217 vs. NCBI nr
Match: XP_008445024.1 (PREDICTED: protein NRT1/ PTR FAMILY 1.2-like isoform X1 [Cucumis melo])

HSP 1 Score: 853.6 bits (2204), Expect = 9.9e-244
Identity = 434/581 (74.70%), Postives = 489/581 (84.17%), Query Frame = 0

Query: 1   MEAPSTADEDRDMEEPLLK-SSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVY 60
           ME P   DE+R MEEPLL  + DGKGG+RTLPFIIAN ALEK+ASQGL+PSMILYLT+VY
Sbjct: 1   MEDPLVVDENRGMEEPLLSDTDDGKGGVRTLPFIIANGALEKLASQGLSPSMILYLTQVY 60

Query: 61  GMESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIP 120
           GM+SA AS VIFLWSAASNFTPIICAFLADSY GRFP++AAGS+ SFLGMFVLWLT  IP
Sbjct: 61  GMKSAQASNVIFLWSAASNFTPIICAFLADSYFGRFPMVAAGSIFSFLGMFVLWLTVMIP 120

Query: 121 QARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLK----SSGT 180
           QARP CDE++GHCNAPS PQLLLLYSSYA+M+IGSGCLQ+S +AFGADQL +     SG 
Sbjct: 121 QARPLCDEINGHCNAPSIPQLLLLYSSYAIMSIGSGCLQASYLAFGADQLYRKNKSKSGI 180

Query: 181 LKNYFSSVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKS 240
           L NYF+  Y++ + G LVGMSCIVYIQD +GW MGFGVPV  ML ++I FLSAS LY+KS
Sbjct: 181 LDNYFNICYISAALGTLVGMSCIVYIQDRMGWGMGFGVPVVLMLLATITFLSASSLYLKS 240

Query: 241 MPSSSLCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISN 300
           MPS S C GLVQVV A+YKKR M++    T  +YHH NGS   +PSDKLRFLNKACII N
Sbjct: 241 MPSKSWCAGLVQVVFAAYKKRHMQIAFVGTSGMYHHENGSLCALPSDKLRFLNKACIIRN 300

Query: 301 SEEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDR 360
           SEEELTSDG+ASNPWSLCTV+QVE LKALI+I+PLWSTGILV+  +NQSFYVLQLASMDR
Sbjct: 301 SEEELTSDGKASNPWSLCTVEQVENLKALIKIIPLWSTGILVSASLNQSFYVLQLASMDR 360

Query: 361 HLTPTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCA 420
           HLT +FEVPAGSFG +L+VSLIIWI LYDRLILPL SK RGK TRLS KTRMG+GIL C 
Sbjct: 361 HLTSSFEVPAGSFGAMLVVSLIIWITLYDRLILPLASKCRGKPTRLSAKTRMGLGILCCT 420

Query: 421 FSLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFY 480
            SL  +A+VE  RRA+AI+EGFSDDP AVVSMSAFWTLPRYI  GM+E F+A+GQIEFFY
Sbjct: 421 LSLAVSAVVEGYRRALAIREGFSDDPNAVVSMSAFWTLPRYILFGMAEAFNAIGQIEFFY 480

Query: 481 FELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYW 540
            ELP+AMSSVATSL GL  SVGNLAASFIMT VDNFSK  GG+SWVS+NIN+GH DYYYW
Sbjct: 481 NELPKAMSSVATSLLGLNSSVGNLAASFIMTTVDNFSKAVGGKSWVSSNINDGHSDYYYW 540

Query: 541 LLFALVFANFLYFLVCSKSYGPSKEEASDESNAEDENNILH 577
           LLF L+FAN LYFL CSKSYGPSKEE+   S AED NN ++
Sbjct: 541 LLFGLLFANLLYFLACSKSYGPSKEESGGRSTAEDYNNAVN 581

BLAST of MS001217 vs. NCBI nr
Match: XP_023546647.1 (protein NRT1/ PTR FAMILY 1.2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 850.5 bits (2196), Expect = 8.4e-243
Identity = 435/577 (75.39%), Postives = 487/577 (84.40%), Query Frame = 0

Query: 1   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 60
           ME P  ADEDRDMEEPLL +SD KGGLR LPFIIAN ALE++ASQGL+PSMILYLTRVYG
Sbjct: 1   MEGPLAADEDRDMEEPLLSNSDRKGGLRALPFIIANGALERLASQGLSPSMILYLTRVYG 60

Query: 61  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 120
           M SA AS VIFLWSAASNFTPIICAFLADSY GRF  IAAG ++S LGMFVLWLTA IPQ
Sbjct: 61  MNSANASNVIFLWSAASNFTPIICAFLADSYFGRFRTIAAGCIISCLGMFVLWLTAMIPQ 120

Query: 121 ARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLK----SSGTL 180
           ARPFCD     C+ PS  QLL LYSSYALM++GSGCLQSS++AFGADQL +    +SG L
Sbjct: 121 ARPFCD--GAICDTPSIAQLLFLYSSYALMSMGSGCLQSSNLAFGADQLHRRNKSNSGIL 180

Query: 181 KNYFSSVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSM 240
             YF   Y++ ++G L+G+SCIVYIQD +GW +GFGVPVA ML S++ FL ASPLY+K M
Sbjct: 181 DRYFDLYYVSSASGSLIGVSCIVYIQDRMGWRVGFGVPVALMLLSTVIFLLASPLYLKPM 240

Query: 241 PSSSLCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNS 300
           PSSS C GLVQV VA+YKKR M+V ST T E+YHH NGSP  MPSDKLRFLNKACII NS
Sbjct: 241 PSSSWCAGLVQVFVAAYKKRHMQVSSTGTSEMYHHKNGSPLAMPSDKLRFLNKACIIRNS 300

Query: 301 EEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDRH 360
           E+EL SDGRASNPW LCTV+QVE LK LIRI+ LWSTGILV   ++Q FYVLQ+ASMDRH
Sbjct: 301 EDELASDGRASNPWILCTVEQVEDLKTLIRIMALWSTGILVCAALSQPFYVLQVASMDRH 360

Query: 361 LTPTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCAF 420
           LTPTFEVPAGSFG V +VSLI+WI+LYDRLILPLVS  RGK TRLS KTRMGIGIL C F
Sbjct: 361 LTPTFEVPAGSFGAVFVVSLILWIMLYDRLILPLVSSCRGKPTRLSGKTRMGIGILLCTF 420

Query: 421 SLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYF 480
           SL  TA+VE+NRRA+AIKEGFSDDP+AVV+MSAFWTLPRYI LGM+E F+ +GQIEFFY+
Sbjct: 421 SLAVTAVVESNRRALAIKEGFSDDPDAVVNMSAFWTLPRYILLGMAEAFNVIGQIEFFYY 480

Query: 481 ELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWL 540
           ELP+AMSSVATSLFGL MSVGNLAASFI+TIVDNF+K  G +SWVS+NIN GH DYYYWL
Sbjct: 481 ELPKAMSSVATSLFGLSMSVGNLAASFIVTIVDNFTKAAGVKSWVSSNINQGHSDYYYWL 540

Query: 541 LFALVFANFLYFLVCSKSYGPSKEEASDESNAEDENN 574
           LF L+FANFLYFL CSKSYGPSKEEA   SNAED+ N
Sbjct: 541 LFGLLFANFLYFLACSKSYGPSKEEARGGSNAEDDLN 575

BLAST of MS001217 vs. ExPASy Swiss-Prot
Match: Q9M817 (Protein NRT1/ PTR FAMILY 1.2 OS=Arabidopsis thaliana OX=3702 GN=NPF1.2 PE=1 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 8.1e-156
Identity = 286/570 (50.18%), Postives = 400/570 (70.18%), Query Frame = 0

Query: 1   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 60
           ME P    E + ++    K +  KGG+ T+PFIIAN A EKVAS GL P+MI+YL R Y 
Sbjct: 1   MENPPNETEAKQIQTNEGKKT--KGGIITMPFIIANEAFEKVASYGLLPNMIMYLIRDYR 60

Query: 61  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 120
              A  + V+F+WSAASNFTP++ AFL+DSYLGRF  I+  S+ SFLGM +LWLTA +PQ
Sbjct: 61  FGVAKGTNVLFMWSAASNFTPLLGAFLSDSYLGRFLTISIASLSSFLGMVLLWLTAMLPQ 120

Query: 121 ARPF-CDELS--GHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQL-----LKS 180
            +P  CD  +   HC + +  QL LLYS++AL++IGSG ++  S+AFGADQL      K+
Sbjct: 121 VKPSPCDPTAAGSHCGSSTASQLALLYSAFALISIGSGGIRPCSLAFGADQLDNKENPKN 180

Query: 181 SGTLKNYFSSVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLY 240
              L+++F   Y + +  VL+  + IVYIQ+H+GW +GFGVP   ML +++ F+ ASPLY
Sbjct: 181 ERVLESFFGWYYASSAVAVLIAFTGIVYIQEHLGWKIGFGVPAVLMLIAALLFILASPLY 240

Query: 241 VKSMPSSSLCTGLVQVVVASYKKRRMKVPS-TDTYEIYHHNNGSPHLMPSDKLRFLNKAC 300
           V    + SL TGL Q +VA+YKKR++ +P   D+++ Y+H   S    PS KLRFLNKAC
Sbjct: 241 VTRGVTKSLFTGLAQAIVAAYKKRKLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNKAC 300

Query: 301 IISNSEEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQ-SFYVLQL 360
           +ISN EEE+ SDG A NPW LCT D+VE LKALI+++P+WSTGI++++  +Q SF +LQ 
Sbjct: 301 LISNREEEIGSDGFALNPWRLCTTDKVEELKALIKVIPIWSTGIMMSINTSQSSFQLLQA 360

Query: 361 ASMDRHLT---PTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRM 420
            SMDR L+    +F+VPAGSFG+  I++L +W+ILYDR ++PL SKIRG+  RLS K RM
Sbjct: 361 TSMDRRLSRHGSSFQVPAGSFGMFTIIALALWVILYDRAVIPLASKIRGRPFRLSVKLRM 420

Query: 421 GIGILFCAFSLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSA 480
           G+G+     ++  +A+VE+ RR  AI +G++++  AVV +SA W +P+Y+  G++E  +A
Sbjct: 421 GLGLFMSFLAMAISAMVESFRRKKAISQGYANNSNAVVDISAMWLVPQYVLHGLAEALTA 480

Query: 481 VGQIEFFYFELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINN 540
           +GQ EFFY E P++MSS+A SLFGLGM+V +L AS ++  V+  +   G ESWVS NIN 
Sbjct: 481 IGQTEFFYTEFPKSMSSIAASLFGLGMAVASLLASVVLNAVNELTSRNGKESWVSDNINK 540

Query: 541 GHCDYYYWLLFALVFANFLYFLVCSKSYGP 558
           GH +YYYW+L  + F N +Y+++CS SYGP
Sbjct: 541 GHYNYYYWVLAIMSFINVIYYVICSWSYGP 568

BLAST of MS001217 vs. ExPASy Swiss-Prot
Match: Q8LPL2 (Protein NRT1/ PTR FAMILY 1.1 OS=Arabidopsis thaliana OX=3702 GN=NPF1.1 PE=1 SV=2)

HSP 1 Score: 533.9 bits (1374), Expect = 2.3e-150
Identity = 284/576 (49.31%), Postives = 389/576 (67.53%), Query Frame = 0

Query: 1   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 60
           ME P    E ++  +  +     KGGL T+PFIIAN   EKVAS GL  +MILYL   Y 
Sbjct: 1   MENPPDQTESKETLQQPITRRRTKGGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYR 60

Query: 61  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 120
           +      TV+F+W AA+NF P++ AFL+DSYLGRF  I   S+ S LGM VLWLTA +PQ
Sbjct: 61  LGLVKGQTVLFMWVAATNFMPLVGAFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQ 120

Query: 121 ARPF-CDELSG-HCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQL-----LKSS 180
            +P  C   +G +C++ ++ QL LLY+++AL++IGSG ++  S+AFGADQL      K+ 
Sbjct: 121 VKPSPCVATAGTNCSSATSSQLALLYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNE 180

Query: 181 GTLKNYFSSVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYV 240
             L+++F   Y + S  VL+  + IVYIQDH+GW +GFG+P   ML +   F+ ASPLYV
Sbjct: 181 RVLESFFGWYYASSSVAVLIAFTVIVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYV 240

Query: 241 KSMPSSSLCTGLVQVVVASYKKRRMKVPS-TDTYEIYHHNNGSPHLMPSDKLRFLNKACI 300
           K   S SL TGL QVV A+Y KR + +P   D+ + Y+    S    PSDKLRFLNKAC 
Sbjct: 241 KRDVSKSLFTGLAQVVAAAYVKRNLTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACA 300

Query: 301 ISNSEEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQ-SFYVLQLA 360
           ISN +E+L SDG A N W LCT DQVE LKAL++++P+WSTGI++++ V+Q SF +LQ  
Sbjct: 301 ISNRDEDLGSDGLALNQWRLCTTDQVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAK 360

Query: 361 SMDRHLT--PTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGI 420
           SMDR L+   TF++PAGSFG+  I++LI W++LYDR ILPL SKIRG+  R++ K RMG+
Sbjct: 361 SMDRRLSSNSTFQIPAGSFGMFTIIALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGL 420

Query: 421 GILFCAFSLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVG 480
           G+     ++  +A VE  RR  AI +G ++D  + VS+SA W +P+Y+  G++E  + +G
Sbjct: 421 GLFISFLAMAVSATVEHYRRKTAISQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIG 480

Query: 481 QIEFFYFELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGH 540
           Q EFFY E P++MSS+A SLFGLGM+V N+ AS I+  V N SK  G  SW+  NIN GH
Sbjct: 481 QTEFFYTEFPKSMSSIAASLFGLGMAVANILASVILNAVKNSSKQ-GNVSWIEDNINKGH 540

Query: 541 CDYYYWLLFALVFANFLYFLVCSKSYGPSKEEASDE 566
            DYYYW+L  L F N +Y++VCS SYGP+ ++  ++
Sbjct: 541 YDYYYWVLAILSFVNVIYYVVCSWSYGPTVDQVRND 575

BLAST of MS001217 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 1.5e-96
Identity = 200/565 (35.40%), Postives = 329/565 (58.23%), Query Frame = 0

Query: 25  GGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYGMESAAASTVIFLWSAASNFTPIIC 84
           GG R + FI+ N  LE++ S GL  + ++YLT+V+ +E   A+ VI +WS  +N TP++ 
Sbjct: 53  GGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVG 112

Query: 85  AFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQARPFC----DELSGHCNAPSTPQL 144
           A+++D+Y+GRF  IA  S  + LG+  + LTA+ PQ  P      D LS  C  P+  Q+
Sbjct: 113 AYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLS--CGGPNKLQI 172

Query: 145 LLLYSSYALMAIGSGCLQSSSIAFGADQLLKSSGT----LKNYFSSVYMAQSAGVLVGMS 204
            +L      +++GSG ++  SI FG DQ  + +      + ++F+  YM  +  +++  +
Sbjct: 173 GVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQT 232

Query: 205 CIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSSLCTGLVQVVVASYKKR 264
            +VYIQD V W +GF +P   M  + + F +    YV   P  S+ +G+ QV+VA+ KKR
Sbjct: 233 VVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKR 292

Query: 265 RMKVPSTDTYEIYHH-----NNGSPHLMPSDKLRFLNKACIISNSEEELTSDGRASNPWS 324
           ++K+P+ D   + ++     ++    L  S++ R L+KA ++   E +LT +G  ++ W 
Sbjct: 293 KLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVV--IEGDLTPEGPPADKWR 352

Query: 325 LCTVDQVEGLKALIRIVPLWSTGI--LVAVGVNQSFYVLQLASMDRHLTPTFEVPAGSFG 384
           LC+V +VE +K LIRIVP+WS GI  L A+    +F V Q   MDR+L P FE+PAGS  
Sbjct: 353 LCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLS 412

Query: 385 VVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCAFSLVATAIVETNRR 444
           V+ ++++ I++  YDR+ +P + +I G ++ ++   R+G GI+F  FS++   IVE  RR
Sbjct: 413 VISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRR 472

Query: 445 AMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPRAMSSVATSL 504
             +I  G   DP  +  MS FW  P+ I +G+ E F+ +GQIEFF  + P  M S+A SL
Sbjct: 473 IRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSL 532

Query: 505 FGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWLLFALVFANFLYFL 564
           F L  +  +  +SF++T+V  FS       W++ N+N G  DY+Y+L+  L   N +YF 
Sbjct: 533 FSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVVNLVYFW 592

Query: 565 VCSKSY----GPSKEEASDESNAED 571
            C++ Y    G   E+  ++ +++D
Sbjct: 593 YCARGYRYKVGLPIEDFEEDKSSDD 610

BLAST of MS001217 vs. ExPASy Swiss-Prot
Match: Q9LYD5 (Protein NRT1/ PTR FAMILY 1.3 OS=Arabidopsis thaliana OX=3702 GN=NPF1.3 PE=2 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 8.8e-94
Identity = 218/554 (39.35%), Postives = 305/554 (55.05%), Query Frame = 0

Query: 12  DMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYGMESAAASTVIF 71
           D E  L+  +  K G+ T+PFI+A+ ALEK+A  GL P+MIL+LT  YGM +A A+ ++F
Sbjct: 2   DQEALLVGRTLLKRGIPTIPFILASQALEKLAYFGLVPNMILFLTVEYGMGTAEAANILF 61

Query: 72  LWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQARPFCDELSGH 131
           LWSAA+NF P++ AF+ADSY GRFP+I  GS +S  GM +LWLT  I   RP CD+L+  
Sbjct: 62  LWSAATNFFPLVGAFIADSYTGRFPLIGFGSSISLTGMVLLWLTTII---RPECDKLTNV 121

Query: 132 CNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLK------SSGTLKNYFSSVYM 191
           C   +  + +LLYS +AL AIG+G ++SS +AF ADQL        ++ +L+  F+  Y 
Sbjct: 122 CQPTTLLKSVLLYSFFALTAIGAGGVRSSCLAFAADQLQPNQTSRVTTSSLETLFNWYYF 181

Query: 192 AQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSSLCTGL 251
           +      +  S +V++Q   GW +GFGV VA M  S   F +ASP YV            
Sbjct: 182 SVMVACFLSQSLLVFVQTTYGWQIGFGVSVAAMALSVALFFAASPYYV------------ 241

Query: 252 VQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNSEEELTSDGR 311
                      R + P+ +                                         
Sbjct: 242 -----------RFQKPTRN----------------------------------------- 301

Query: 312 ASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAV--GVNQSFYVLQLASMDRH-LTPTFE 371
           + NPW LC V QVE LK+LI ++P+WSTGI++++      SF VLQ  +MDRH     FE
Sbjct: 302 SRNPWKLCRVQQVEDLKSLINVIPIWSTGIILSLVTACQVSFIVLQAKTMDRHTFIQGFE 361

Query: 372 VPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCAFSLVATA 431
           +P GS+G+ L++S ++++ LYD +I+PL+S    +  RL    RM  G +     + A A
Sbjct: 362 IPPGSYGIFLVISFLLFLGLYDLVIVPLLSWALREPFRLGVMVRMWAGYVISVLCISALA 421

Query: 432 IVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPRAM 491
             E  RR  A         E+   +SA W LP  I  G++E  + + Q EFFY ELP+ M
Sbjct: 422 ATEYARRKTA-------RDESGTKLSAMWLLPYMILGGIAEALNTIAQNEFFYSELPKTM 477

Query: 492 SSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWLLFALVF 551
           SSVAT+L  L M+  +L +S+I+TIVD    T G  SW++ NI+ GH DYYYWLL  L  
Sbjct: 482 SSVATTLSSLNMAAASLISSWIITIVD--VTTYG--SWITENIDEGHLDYYYWLLVGLSL 477

Query: 552 ANFLYFLVCSKSYG 557
            N LYF+ C KSYG
Sbjct: 542 LNVLYFVWCKKSYG 477

BLAST of MS001217 vs. ExPASy Swiss-Prot
Match: Q9SX20 (Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 3.3e-93
Identity = 199/544 (36.58%), Postives = 318/544 (58.46%), Query Frame = 0

Query: 24  KGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYGMESAAASTVIFLWSAASNFTPII 83
           KGGL T+PFI AN   EK+A  G   +MI YLT    +    A+  +  ++  S+ TP++
Sbjct: 24  KGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLHLPLTKAANTLTNFAGTSSLTPLL 83

Query: 84  CAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQARPFCDELSGHCNAPSTPQLLLL 143
            AF+ADS+ GRF  I   S++  +GM +L ++A IP  RP   +    C    T QL +L
Sbjct: 84  GAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPTLRPPPCKGEEVCVVADTAQLSIL 143

Query: 144 YSSYALMAIGSGCLQSSSIAFGADQLLKS--SGTLK--NYFSSVYMAQSAGVLVGMSCIV 203
           Y +  L A+GSG ++   +AFGADQ  +S  + T K  NYF+  Y    A VL+ ++ +V
Sbjct: 144 YVALLLGALGSGGIRPCVVAFGADQFDESDPNQTTKTWNYFNWYYFCMGAAVLLAVTVLV 203

Query: 204 YIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSSLCTGLVQVVVASYKKRRMK 263
           +IQD+VGW +G G+P   M  S IAF+    LY   +P+ S  T L+QV VA+++KR+++
Sbjct: 204 WIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRLIQVGVAAFRKRKLR 263

Query: 264 VPSTDTYEIYHHNNGSP-----HLMPSDKLRFLNKACIISNSEEELTSDGRASNPWSLCT 323
           + S  +   ++    +P      L  +  + FL+KA I+  +EE+    G+  N W L T
Sbjct: 264 MVSDPSLLYFNDEIDAPISLGGKLTHTKHMSFLDKAAIV--TEEDNLKPGQIPNHWRLST 323

Query: 324 VDQVEGLKALIRIVPLWSTGILVAVGVNQ--SFYVLQLASMDRHLTPTFEVPAGSFGVVL 383
           V +VE LK++IR+ P+ ++GIL+     Q  +F + Q  +M+RHLT +F++PAGS  V  
Sbjct: 324 VHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRHLTNSFQIPAGSMSVFT 383

Query: 384 IVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCAFSLVATAIVETNRRAMA 443
            V+++  II YDR+ + +  K  G +  ++   RMGIG +    + +    VE  R+++A
Sbjct: 384 TVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISIIATLVAGFVEVKRKSVA 443

Query: 444 IKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPRAMSSVATSLFGL 503
           I+ G  D P  +V +S  W +P+Y   G++E F ++G +EFFY + P +M S AT+LF +
Sbjct: 444 IEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQAPESMRSTATALFWM 503

Query: 504 GMSVGNLAASFIMTIVDNFSKTGGGESWV-STNINNGHCDYYYWLLFALVFANFLYFLVC 556
            +S+GN  ++ ++T+V  FS    G +W+   N+N G  +Y+YWL+  L   N +Y+L C
Sbjct: 504 AISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYWLITVLQAVNLVYYLWC 563

BLAST of MS001217 vs. ExPASy TrEMBL
Match: A0A6J1BUQ4 (protein NRT1/ PTR FAMILY 1.2-like OS=Momordica charantia OX=3673 GN=LOC111004912 PE=3 SV=1)

HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 574/576 (99.65%), Postives = 574/576 (99.65%), Query Frame = 0

Query: 1   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 60
           MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG
Sbjct: 1   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 60

Query: 61  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 120
           MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ
Sbjct: 61  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 120

Query: 121 ARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLKSSGTLKNYF 180
           ARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLKSSGTLKNYF
Sbjct: 121 ARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLKSSGTLKNYF 180

Query: 181 SSVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSS 240
           S VYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSS
Sbjct: 181 SLVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSS 240

Query: 241 LCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNSEEEL 300
           LCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNSEEEL
Sbjct: 241 LCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNSEEEL 300

Query: 301 TSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDRHLTPT 360
           TSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDRHLTPT
Sbjct: 301 TSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDRHLTPT 360

Query: 361 FEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCAFSLVA 420
           FEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFC FSLVA
Sbjct: 361 FEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCTFSLVA 420

Query: 421 TAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPR 480
           TAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPR
Sbjct: 421 TAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPR 480

Query: 481 AMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWLLFAL 540
           AMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWLLFAL
Sbjct: 481 AMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWLLFAL 540

Query: 541 VFANFLYFLVCSKSYGPSKEEASDESNAEDENNILH 577
           VFANFLYFLVCSKSYGPSKEEASDESNAEDENNILH
Sbjct: 541 VFANFLYFLVCSKSYGPSKEEASDESNAEDENNILH 576

BLAST of MS001217 vs. ExPASy TrEMBL
Match: A0A1S3BBQ7 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488189 PE=3 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 4.8e-244
Identity = 434/581 (74.70%), Postives = 489/581 (84.17%), Query Frame = 0

Query: 1   MEAPSTADEDRDMEEPLLK-SSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVY 60
           ME P   DE+R MEEPLL  + DGKGG+RTLPFIIAN ALEK+ASQGL+PSMILYLT+VY
Sbjct: 1   MEDPLVVDENRGMEEPLLSDTDDGKGGVRTLPFIIANGALEKLASQGLSPSMILYLTQVY 60

Query: 61  GMESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIP 120
           GM+SA AS VIFLWSAASNFTPIICAFLADSY GRFP++AAGS+ SFLGMFVLWLT  IP
Sbjct: 61  GMKSAQASNVIFLWSAASNFTPIICAFLADSYFGRFPMVAAGSIFSFLGMFVLWLTVMIP 120

Query: 121 QARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLK----SSGT 180
           QARP CDE++GHCNAPS PQLLLLYSSYA+M+IGSGCLQ+S +AFGADQL +     SG 
Sbjct: 121 QARPLCDEINGHCNAPSIPQLLLLYSSYAIMSIGSGCLQASYLAFGADQLYRKNKSKSGI 180

Query: 181 LKNYFSSVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKS 240
           L NYF+  Y++ + G LVGMSCIVYIQD +GW MGFGVPV  ML ++I FLSAS LY+KS
Sbjct: 181 LDNYFNICYISAALGTLVGMSCIVYIQDRMGWGMGFGVPVVLMLLATITFLSASSLYLKS 240

Query: 241 MPSSSLCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISN 300
           MPS S C GLVQVV A+YKKR M++    T  +YHH NGS   +PSDKLRFLNKACII N
Sbjct: 241 MPSKSWCAGLVQVVFAAYKKRHMQIAFVGTSGMYHHENGSLCALPSDKLRFLNKACIIRN 300

Query: 301 SEEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDR 360
           SEEELTSDG+ASNPWSLCTV+QVE LKALI+I+PLWSTGILV+  +NQSFYVLQLASMDR
Sbjct: 301 SEEELTSDGKASNPWSLCTVEQVENLKALIKIIPLWSTGILVSASLNQSFYVLQLASMDR 360

Query: 361 HLTPTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCA 420
           HLT +FEVPAGSFG +L+VSLIIWI LYDRLILPL SK RGK TRLS KTRMG+GIL C 
Sbjct: 361 HLTSSFEVPAGSFGAMLVVSLIIWITLYDRLILPLASKCRGKPTRLSAKTRMGLGILCCT 420

Query: 421 FSLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFY 480
            SL  +A+VE  RRA+AI+EGFSDDP AVVSMSAFWTLPRYI  GM+E F+A+GQIEFFY
Sbjct: 421 LSLAVSAVVEGYRRALAIREGFSDDPNAVVSMSAFWTLPRYILFGMAEAFNAIGQIEFFY 480

Query: 481 FELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYW 540
            ELP+AMSSVATSL GL  SVGNLAASFIMT VDNFSK  GG+SWVS+NIN+GH DYYYW
Sbjct: 481 NELPKAMSSVATSLLGLNSSVGNLAASFIMTTVDNFSKAVGGKSWVSSNINDGHSDYYYW 540

Query: 541 LLFALVFANFLYFLVCSKSYGPSKEEASDESNAEDENNILH 577
           LLF L+FAN LYFL CSKSYGPSKEE+   S AED NN ++
Sbjct: 541 LLFGLLFANLLYFLACSKSYGPSKEESGGRSTAEDYNNAVN 581

BLAST of MS001217 vs. ExPASy TrEMBL
Match: A0A6J1HCS0 (protein NRT1/ PTR FAMILY 1.2-like OS=Cucurbita moschata OX=3662 GN=LOC111462799 PE=3 SV=1)

HSP 1 Score: 849.0 bits (2192), Expect = 1.2e-242
Identity = 434/577 (75.22%), Postives = 489/577 (84.75%), Query Frame = 0

Query: 1   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 60
           ME P  ADEDRDMEEPLL +SD KGGLR LPFIIAN ALE++ASQGL+PSMILYLTRVYG
Sbjct: 1   MEGPLAADEDRDMEEPLLSNSDRKGGLRALPFIIANGALERLASQGLSPSMILYLTRVYG 60

Query: 61  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 120
           M SA AS VIFLWSAASNFTPIICAFLADSY GRF +IAAG ++S LGMFVLWLTA IPQ
Sbjct: 61  MNSANASNVIFLWSAASNFTPIICAFLADSYFGRFRMIAAGCIISCLGMFVLWLTAMIPQ 120

Query: 121 ARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLK----SSGTL 180
           ARPFCD     C+ PS  QLLLLYSSYALM++GSGCLQSS++AFGA+QL +    +SG L
Sbjct: 121 ARPFCD--GAICDTPSIAQLLLLYSSYALMSMGSGCLQSSNLAFGANQLHRKNKSNSGIL 180

Query: 181 KNYFSSVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSM 240
             YF   Y++ ++G L+G+SCIVYIQD +GW +GFGVPVA ML S++ FL ASPLY+K M
Sbjct: 181 DRYFDLYYVSSASGSLIGVSCIVYIQDRMGWRVGFGVPVALMLLSTVIFLLASPLYLKPM 240

Query: 241 PSSSLCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNS 300
           PSSS C GLVQV VA+YKKR M+V ST T E+YHH +GSP  MPSDKLRFLNKACII NS
Sbjct: 241 PSSSWCAGLVQVFVAAYKKRHMQVSSTGTSEMYHHKSGSPLAMPSDKLRFLNKACIIRNS 300

Query: 301 EEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDRH 360
           E+EL SDGRASNPW LCTV+QVE LK LIRI+ LWSTGILV   ++Q FYVLQ+ASMDRH
Sbjct: 301 EDELASDGRASNPWILCTVEQVEDLKTLIRIMALWSTGILVCAALSQPFYVLQVASMDRH 360

Query: 361 LTPTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCAF 420
           LTPTFEVPAGSFG V +VSLI+WI+LYDRLILPLVS  RGK TRLS KTRMGIGIL C F
Sbjct: 361 LTPTFEVPAGSFGAVFVVSLILWIMLYDRLILPLVSSCRGKPTRLSGKTRMGIGILLCTF 420

Query: 421 SLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYF 480
           SL  TA+VE+NRRA+AIKEGFSDDP+AVV+MSAFWTLPRYI LGM+E F+ +GQIEFFY+
Sbjct: 421 SLAVTAVVESNRRALAIKEGFSDDPDAVVNMSAFWTLPRYILLGMAEAFNVIGQIEFFYY 480

Query: 481 ELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWL 540
           ELP+AMSSVATSLFGL MSVGNLAASFI+TIVDNF+K  G +SWVS+NIN GH DYYYWL
Sbjct: 481 ELPKAMSSVATSLFGLSMSVGNLAASFIVTIVDNFTKAAGVKSWVSSNINQGHSDYYYWL 540

Query: 541 LFALVFANFLYFLVCSKSYGPSKEEASDESNAEDENN 574
           LF L+FANFLYFL CSKSYGPSKEEA   SNAED+ N
Sbjct: 541 LFGLLFANFLYFLACSKSYGPSKEEARGGSNAEDDLN 575

BLAST of MS001217 vs. ExPASy TrEMBL
Match: A0A6J1KBF9 (protein NRT1/ PTR FAMILY 1.2-like OS=Cucurbita maxima OX=3661 GN=LOC111491785 PE=3 SV=1)

HSP 1 Score: 848.6 bits (2191), Expect = 1.5e-242
Identity = 435/577 (75.39%), Postives = 485/577 (84.06%), Query Frame = 0

Query: 1   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 60
           ME P  ADEDRDMEEPLL +SD K GLR LPFIIAN ALEK+ASQGL+PSMILYLTRVYG
Sbjct: 1   MEGPLAADEDRDMEEPLLSNSDRKEGLRALPFIIANGALEKLASQGLSPSMILYLTRVYG 60

Query: 61  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 120
           M SA AS VIFLWSAASNFTPIICAFLADSY GRF +IAAG ++S LGMFVLWLTA IPQ
Sbjct: 61  MNSANASNVIFLWSAASNFTPIICAFLADSYFGRFRMIAAGCIISCLGMFVLWLTAMIPQ 120

Query: 121 ARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLK----SSGTL 180
           ARPFCD     C+ PS  QLLLLYSSYALM+IGSGCLQSS++AFGADQL +    +SG L
Sbjct: 121 ARPFCD--GAICDTPSIAQLLLLYSSYALMSIGSGCLQSSNLAFGADQLHRKNKSNSGIL 180

Query: 181 KNYFSSVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSM 240
             YF   Y++ ++G L+G+SCIVYIQD +GW +GFGVPVA ML S++ FL ASPLY+K M
Sbjct: 181 DRYFDLYYVSSASGSLIGVSCIVYIQDRMGWRVGFGVPVALMLLSTVIFLLASPLYLKPM 240

Query: 241 PSSSLCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNS 300
           PSSS C GLVQV VA+YKKR M+V ST T E+YHH NGSP  MPSDKLRFLNKACII NS
Sbjct: 241 PSSSWCAGLVQVFVAAYKKRHMQVSSTGTSEMYHHKNGSPLAMPSDKLRFLNKACIIRNS 300

Query: 301 EEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDRH 360
           E+EL SDGRASNPW LCTV+QVE LK LIRI+ LWSTGILV   ++Q FYVLQ+ASMDRH
Sbjct: 301 EDELASDGRASNPWILCTVEQVEDLKTLIRIMALWSTGILVCAALSQPFYVLQVASMDRH 360

Query: 361 LTPTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCAF 420
           LTPTFE+PAGSFG V +VSLI+WI+LYDRLILPLVS  RGK TRLS KTRMGIGIL C F
Sbjct: 361 LTPTFEIPAGSFGAVFVVSLILWIMLYDRLILPLVSSCRGKPTRLSGKTRMGIGILLCTF 420

Query: 421 SLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYF 480
           SL  TA+VE+NRRA+AIKEGFSDDP AVV+MSAFWTLPRYI LGM+E F+ +GQIEFFY+
Sbjct: 421 SLAVTAVVESNRRALAIKEGFSDDPAAVVNMSAFWTLPRYILLGMAEAFNVIGQIEFFYY 480

Query: 481 ELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWL 540
           ELP+AMSSVATSLFGL MSVGNL ASFIMT VDNF+K  G +SWVS+NIN GH DYYYWL
Sbjct: 481 ELPKAMSSVATSLFGLSMSVGNLTASFIMTTVDNFTKAAGVKSWVSSNINQGHSDYYYWL 540

Query: 541 LFALVFANFLYFLVCSKSYGPSKEEASDESNAEDENN 574
           LF LVFANFLYFL CS+SYGPSKEEA   SNAED+ N
Sbjct: 541 LFGLVFANFLYFLACSRSYGPSKEEARGGSNAEDDLN 575

BLAST of MS001217 vs. ExPASy TrEMBL
Match: A0A1S4DVD3 (protein NRT1/ PTR FAMILY 1.2-like OS=Cucumis melo OX=3656 GN=LOC103488191 PE=3 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 5.7e-237
Identity = 423/581 (72.81%), Postives = 488/581 (83.99%), Query Frame = 0

Query: 1   MEAPSTADEDRDMEEPLLK-SSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVY 60
           ME     DE+R MEEPLL  + DGKGGLRTLPFIIAN   EKVAS GL+PSMILYLT VY
Sbjct: 1   MEDQLVVDENRGMEEPLLSDTDDGKGGLRTLPFIIANGTFEKVASHGLSPSMILYLTEVY 60

Query: 61  GMESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIP 120
           GM+SA AS VIFLWSAA+NFTPIICAFLADSY GRF +IAAGS+ SFLGMFVLWLTA IP
Sbjct: 61  GMKSAHASNVIFLWSAATNFTPIICAFLADSYFGRFSMIAAGSIFSFLGMFVLWLTAIIP 120

Query: 121 QARPFCDELSGHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLK----SSGT 180
           Q RPFCD+++G+C+APSTPQLLLLYSSYA+M+IGSGCLQ+S +AFGADQL K    +SG 
Sbjct: 121 QVRPFCDKINGYCDAPSTPQLLLLYSSYAIMSIGSGCLQASHLAFGADQLYKESKSNSGI 180

Query: 181 LKNYFSSVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKS 240
           L +YF+  Y++ + G L+GMSCIVYIQD +GW +GFGV VA ML ++I FLSAS LY+KS
Sbjct: 181 LNSYFNICYISIAVGTLIGMSCIVYIQDRMGWGIGFGVSVALMLLATITFLSASSLYLKS 240

Query: 241 MPSSSLCTGLVQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISN 300
           MPS S C GLVQVV A+YKKR M++P   T+E+YHH  GS   +PS+KLRFLNKACII N
Sbjct: 241 MPSKSWCDGLVQVVFATYKKRHMQIPFVGTFEMYHHETGSLCALPSNKLRFLNKACIIRN 300

Query: 301 SEEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQSFYVLQLASMDR 360
            EEELTSDG+ASNPWSLCTV+QVE LKALIRI+PLWSTGIL +V ++QSFYVLQ+ASMDR
Sbjct: 301 FEEELTSDGKASNPWSLCTVEQVENLKALIRIIPLWSTGILFSVSLSQSFYVLQVASMDR 360

Query: 361 HLTPTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCA 420
           HLT +FEVPAGSF V+++V +IIWIILY+ LILPL SK RGK T LS KTRMG+GIL C 
Sbjct: 361 HLTSSFEVPAGSFSVLIVVFVIIWIILYNCLILPLASKCRGKPTHLSGKTRMGLGILCCT 420

Query: 421 FSLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFY 480
            SL  +AIVE +RRA+AIKEGFS+DP AVVSMSAFWTLPRYI  GM+E F+A+GQIEFFY
Sbjct: 421 LSLAVSAIVEGDRRALAIKEGFSNDPNAVVSMSAFWTLPRYILFGMAEAFNAIGQIEFFY 480

Query: 481 FELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYW 540
            ELP+AMSSVATSL GL  SVGNLAASFIMT VDNFSK  GG+SWVS+NIN+GH DYYYW
Sbjct: 481 NELPKAMSSVATSLLGLNSSVGNLAASFIMTTVDNFSKAVGGKSWVSSNINDGHSDYYYW 540

Query: 541 LLFALVFANFLYFLVCSKSYGPSKEEASDESNAEDENNILH 577
           LLF L+FANFLYFL CSKSYGPSKEE++  S AED NN ++
Sbjct: 541 LLFGLLFANFLYFLACSKSYGPSKEESAGRSTAEDYNNTVN 581

BLAST of MS001217 vs. TAIR 10
Match: AT1G52190.1 (Major facilitator superfamily protein )

HSP 1 Score: 552.0 bits (1421), Expect = 5.8e-157
Identity = 286/570 (50.18%), Postives = 400/570 (70.18%), Query Frame = 0

Query: 1   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 60
           ME P    E + ++    K +  KGG+ T+PFIIAN A EKVAS GL P+MI+YL R Y 
Sbjct: 1   MENPPNETEAKQIQTNEGKKT--KGGIITMPFIIANEAFEKVASYGLLPNMIMYLIRDYR 60

Query: 61  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 120
              A  + V+F+WSAASNFTP++ AFL+DSYLGRF  I+  S+ SFLGM +LWLTA +PQ
Sbjct: 61  FGVAKGTNVLFMWSAASNFTPLLGAFLSDSYLGRFLTISIASLSSFLGMVLLWLTAMLPQ 120

Query: 121 ARPF-CDELS--GHCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQL-----LKS 180
            +P  CD  +   HC + +  QL LLYS++AL++IGSG ++  S+AFGADQL      K+
Sbjct: 121 VKPSPCDPTAAGSHCGSSTASQLALLYSAFALISIGSGGIRPCSLAFGADQLDNKENPKN 180

Query: 181 SGTLKNYFSSVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLY 240
              L+++F   Y + +  VL+  + IVYIQ+H+GW +GFGVP   ML +++ F+ ASPLY
Sbjct: 181 ERVLESFFGWYYASSAVAVLIAFTGIVYIQEHLGWKIGFGVPAVLMLIAALLFILASPLY 240

Query: 241 VKSMPSSSLCTGLVQVVVASYKKRRMKVPS-TDTYEIYHHNNGSPHLMPSDKLRFLNKAC 300
           V    + SL TGL Q +VA+YKKR++ +P   D+++ Y+H   S    PS KLRFLNKAC
Sbjct: 241 VTRGVTKSLFTGLAQAIVAAYKKRKLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNKAC 300

Query: 301 IISNSEEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQ-SFYVLQL 360
           +ISN EEE+ SDG A NPW LCT D+VE LKALI+++P+WSTGI++++  +Q SF +LQ 
Sbjct: 301 LISNREEEIGSDGFALNPWRLCTTDKVEELKALIKVIPIWSTGIMMSINTSQSSFQLLQA 360

Query: 361 ASMDRHLT---PTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRM 420
            SMDR L+    +F+VPAGSFG+  I++L +W+ILYDR ++PL SKIRG+  RLS K RM
Sbjct: 361 TSMDRRLSRHGSSFQVPAGSFGMFTIIALALWVILYDRAVIPLASKIRGRPFRLSVKLRM 420

Query: 421 GIGILFCAFSLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSA 480
           G+G+     ++  +A+VE+ RR  AI +G++++  AVV +SA W +P+Y+  G++E  +A
Sbjct: 421 GLGLFMSFLAMAISAMVESFRRKKAISQGYANNSNAVVDISAMWLVPQYVLHGLAEALTA 480

Query: 481 VGQIEFFYFELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINN 540
           +GQ EFFY E P++MSS+A SLFGLGM+V +L AS ++  V+  +   G ESWVS NIN 
Sbjct: 481 IGQTEFFYTEFPKSMSSIAASLFGLGMAVASLLASVVLNAVNELTSRNGKESWVSDNINK 540

Query: 541 GHCDYYYWLLFALVFANFLYFLVCSKSYGP 558
           GH +YYYW+L  + F N +Y+++CS SYGP
Sbjct: 541 GHYNYYYWVLAIMSFINVIYYVICSWSYGP 568

BLAST of MS001217 vs. TAIR 10
Match: AT3G16180.1 (Major facilitator superfamily protein )

HSP 1 Score: 533.9 bits (1374), Expect = 1.6e-151
Identity = 284/576 (49.31%), Postives = 389/576 (67.53%), Query Frame = 0

Query: 1   MEAPSTADEDRDMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYG 60
           ME P    E ++  +  +     KGGL T+PFIIAN   EKVAS GL  +MILYL   Y 
Sbjct: 1   MENPPDQTESKETLQQPITRRRTKGGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYR 60

Query: 61  MESAAASTVIFLWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQ 120
           +      TV+F+W AA+NF P++ AFL+DSYLGRF  I   S+ S LGM VLWLTA +PQ
Sbjct: 61  LGLVKGQTVLFMWVAATNFMPLVGAFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQ 120

Query: 121 ARPF-CDELSG-HCNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQL-----LKSS 180
            +P  C   +G +C++ ++ QL LLY+++AL++IGSG ++  S+AFGADQL      K+ 
Sbjct: 121 VKPSPCVATAGTNCSSATSSQLALLYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNE 180

Query: 181 GTLKNYFSSVYMAQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYV 240
             L+++F   Y + S  VL+  + IVYIQDH+GW +GFG+P   ML +   F+ ASPLYV
Sbjct: 181 RVLESFFGWYYASSSVAVLIAFTVIVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYV 240

Query: 241 KSMPSSSLCTGLVQVVVASYKKRRMKVPS-TDTYEIYHHNNGSPHLMPSDKLRFLNKACI 300
           K   S SL TGL QVV A+Y KR + +P   D+ + Y+    S    PSDKLRFLNKAC 
Sbjct: 241 KRDVSKSLFTGLAQVVAAAYVKRNLTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACA 300

Query: 301 ISNSEEELTSDGRASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAVGVNQ-SFYVLQLA 360
           ISN +E+L SDG A N W LCT DQVE LKAL++++P+WSTGI++++ V+Q SF +LQ  
Sbjct: 301 ISNRDEDLGSDGLALNQWRLCTTDQVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAK 360

Query: 361 SMDRHLT--PTFEVPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGI 420
           SMDR L+   TF++PAGSFG+  I++LI W++LYDR ILPL SKIRG+  R++ K RMG+
Sbjct: 361 SMDRRLSSNSTFQIPAGSFGMFTIIALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGL 420

Query: 421 GILFCAFSLVATAIVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVG 480
           G+     ++  +A VE  RR  AI +G ++D  + VS+SA W +P+Y+  G++E  + +G
Sbjct: 421 GLFISFLAMAVSATVEHYRRKTAISQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIG 480

Query: 481 QIEFFYFELPRAMSSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGH 540
           Q EFFY E P++MSS+A SLFGLGM+V N+ AS I+  V N SK  G  SW+  NIN GH
Sbjct: 481 QTEFFYTEFPKSMSSIAASLFGLGMAVANILASVILNAVKNSSKQ-GNVSWIEDNINKGH 540

Query: 541 CDYYYWLLFALVFANFLYFLVCSKSYGPSKEEASDE 566
            DYYYW+L  L F N +Y++VCS SYGP+ ++  ++
Sbjct: 541 YDYYYWVLAILSFVNVIYYVVCSWSYGPTVDQVRND 575

BLAST of MS001217 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 355.1 bits (910), Expect = 1.0e-97
Identity = 200/565 (35.40%), Postives = 329/565 (58.23%), Query Frame = 0

Query: 25  GGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYGMESAAASTVIFLWSAASNFTPIIC 84
           GG R + FI+ N  LE++ S GL  + ++YLT+V+ +E   A+ VI +WS  +N TP++ 
Sbjct: 53  GGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVG 112

Query: 85  AFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQARPFC----DELSGHCNAPSTPQL 144
           A+++D+Y+GRF  IA  S  + LG+  + LTA+ PQ  P      D LS  C  P+  Q+
Sbjct: 113 AYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLS--CGGPNKLQI 172

Query: 145 LLLYSSYALMAIGSGCLQSSSIAFGADQLLKSSGT----LKNYFSSVYMAQSAGVLVGMS 204
            +L      +++GSG ++  SI FG DQ  + +      + ++F+  YM  +  +++  +
Sbjct: 173 GVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQT 232

Query: 205 CIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSSLCTGLVQVVVASYKKR 264
            +VYIQD V W +GF +P   M  + + F +    YV   P  S+ +G+ QV+VA+ KKR
Sbjct: 233 VVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKR 292

Query: 265 RMKVPSTDTYEIYHH-----NNGSPHLMPSDKLRFLNKACIISNSEEELTSDGRASNPWS 324
           ++K+P+ D   + ++     ++    L  S++ R L+KA ++   E +LT +G  ++ W 
Sbjct: 293 KLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVV--IEGDLTPEGPPADKWR 352

Query: 325 LCTVDQVEGLKALIRIVPLWSTGI--LVAVGVNQSFYVLQLASMDRHLTPTFEVPAGSFG 384
           LC+V +VE +K LIRIVP+WS GI  L A+    +F V Q   MDR+L P FE+PAGS  
Sbjct: 353 LCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLS 412

Query: 385 VVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCAFSLVATAIVETNRR 444
           V+ ++++ I++  YDR+ +P + +I G ++ ++   R+G GI+F  FS++   IVE  RR
Sbjct: 413 VISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRR 472

Query: 445 AMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPRAMSSVATSL 504
             +I  G   DP  +  MS FW  P+ I +G+ E F+ +GQIEFF  + P  M S+A SL
Sbjct: 473 IRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSL 532

Query: 505 FGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWLLFALVFANFLYFL 564
           F L  +  +  +SF++T+V  FS       W++ N+N G  DY+Y+L+  L   N +YF 
Sbjct: 533 FSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVVNLVYFW 592

Query: 565 VCSKSY----GPSKEEASDESNAED 571
            C++ Y    G   E+  ++ +++D
Sbjct: 593 YCARGYRYKVGLPIEDFEEDKSSDD 610

BLAST of MS001217 vs. TAIR 10
Match: AT5G11570.1 (Major facilitator superfamily protein )

HSP 1 Score: 345.9 bits (886), Expect = 6.3e-95
Identity = 218/554 (39.35%), Postives = 305/554 (55.05%), Query Frame = 0

Query: 12  DMEEPLLKSSDGKGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYGMESAAASTVIF 71
           D E  L+  +  K G+ T+PFI+A+ ALEK+A  GL P+MIL+LT  YGM +A A+ ++F
Sbjct: 2   DQEALLVGRTLLKRGIPTIPFILASQALEKLAYFGLVPNMILFLTVEYGMGTAEAANILF 61

Query: 72  LWSAASNFTPIICAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQARPFCDELSGH 131
           LWSAA+NF P++ AF+ADSY GRFP+I  GS +S  GM +LWLT  I   RP CD+L+  
Sbjct: 62  LWSAATNFFPLVGAFIADSYTGRFPLIGFGSSISLTGMVLLWLTTII---RPECDKLTNV 121

Query: 132 CNAPSTPQLLLLYSSYALMAIGSGCLQSSSIAFGADQLLK------SSGTLKNYFSSVYM 191
           C   +  + +LLYS +AL AIG+G ++SS +AF ADQL        ++ +L+  F+  Y 
Sbjct: 122 CQPTTLLKSVLLYSFFALTAIGAGGVRSSCLAFAADQLQPNQTSRVTTSSLETLFNWYYF 181

Query: 192 AQSAGVLVGMSCIVYIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSSLCTGL 251
           +      +  S +V++Q   GW +GFGV VA M  S   F +ASP YV            
Sbjct: 182 SVMVACFLSQSLLVFVQTTYGWQIGFGVSVAAMALSVALFFAASPYYV------------ 241

Query: 252 VQVVVASYKKRRMKVPSTDTYEIYHHNNGSPHLMPSDKLRFLNKACIISNSEEELTSDGR 311
                      R + P+ +                                         
Sbjct: 242 -----------RFQKPTRN----------------------------------------- 301

Query: 312 ASNPWSLCTVDQVEGLKALIRIVPLWSTGILVAV--GVNQSFYVLQLASMDRH-LTPTFE 371
           + NPW LC V QVE LK+LI ++P+WSTGI++++      SF VLQ  +MDRH     FE
Sbjct: 302 SRNPWKLCRVQQVEDLKSLINVIPIWSTGIILSLVTACQVSFIVLQAKTMDRHTFIQGFE 361

Query: 372 VPAGSFGVVLIVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCAFSLVATA 431
           +P GS+G+ L++S ++++ LYD +I+PL+S    +  RL    RM  G +     + A A
Sbjct: 362 IPPGSYGIFLVISFLLFLGLYDLVIVPLLSWALREPFRLGVMVRMWAGYVISVLCISALA 421

Query: 432 IVETNRRAMAIKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPRAM 491
             E  RR  A         E+   +SA W LP  I  G++E  + + Q EFFY ELP+ M
Sbjct: 422 ATEYARRKTA-------RDESGTKLSAMWLLPYMILGGIAEALNTIAQNEFFYSELPKTM 477

Query: 492 SSVATSLFGLGMSVGNLAASFIMTIVDNFSKTGGGESWVSTNINNGHCDYYYWLLFALVF 551
           SSVAT+L  L M+  +L +S+I+TIVD    T G  SW++ NI+ GH DYYYWLL  L  
Sbjct: 482 SSVATTLSSLNMAAASLISSWIITIVD--VTTYG--SWITENIDEGHLDYYYWLLVGLSL 477

Query: 552 ANFLYFLVCSKSYG 557
            N LYF+ C KSYG
Sbjct: 542 LNVLYFVWCKKSYG 477

BLAST of MS001217 vs. TAIR 10
Match: AT1G68570.1 (Major facilitator superfamily protein )

HSP 1 Score: 344.0 bits (881), Expect = 2.4e-94
Identity = 199/544 (36.58%), Postives = 318/544 (58.46%), Query Frame = 0

Query: 24  KGGLRTLPFIIANVALEKVASQGLTPSMILYLTRVYGMESAAASTVIFLWSAASNFTPII 83
           KGGL T+PFI AN   EK+A  G   +MI YLT    +    A+  +  ++  S+ TP++
Sbjct: 24  KGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLHLPLTKAANTLTNFAGTSSLTPLL 83

Query: 84  CAFLADSYLGRFPIIAAGSVVSFLGMFVLWLTATIPQARPFCDELSGHCNAPSTPQLLLL 143
            AF+ADS+ GRF  I   S++  +GM +L ++A IP  RP   +    C    T QL +L
Sbjct: 84  GAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPTLRPPPCKGEEVCVVADTAQLSIL 143

Query: 144 YSSYALMAIGSGCLQSSSIAFGADQLLKS--SGTLK--NYFSSVYMAQSAGVLVGMSCIV 203
           Y +  L A+GSG ++   +AFGADQ  +S  + T K  NYF+  Y    A VL+ ++ +V
Sbjct: 144 YVALLLGALGSGGIRPCVVAFGADQFDESDPNQTTKTWNYFNWYYFCMGAAVLLAVTVLV 203

Query: 204 YIQDHVGWAMGFGVPVACMLFSSIAFLSASPLYVKSMPSSSLCTGLVQVVVASYKKRRMK 263
           +IQD+VGW +G G+P   M  S IAF+    LY   +P+ S  T L+QV VA+++KR+++
Sbjct: 204 WIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRLIQVGVAAFRKRKLR 263

Query: 264 VPSTDTYEIYHHNNGSP-----HLMPSDKLRFLNKACIISNSEEELTSDGRASNPWSLCT 323
           + S  +   ++    +P      L  +  + FL+KA I+  +EE+    G+  N W L T
Sbjct: 264 MVSDPSLLYFNDEIDAPISLGGKLTHTKHMSFLDKAAIV--TEEDNLKPGQIPNHWRLST 323

Query: 324 VDQVEGLKALIRIVPLWSTGILVAVGVNQ--SFYVLQLASMDRHLTPTFEVPAGSFGVVL 383
           V +VE LK++IR+ P+ ++GIL+     Q  +F + Q  +M+RHLT +F++PAGS  V  
Sbjct: 324 VHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRHLTNSFQIPAGSMSVFT 383

Query: 384 IVSLIIWIILYDRLILPLVSKIRGKQTRLSRKTRMGIGILFCAFSLVATAIVETNRRAMA 443
            V+++  II YDR+ + +  K  G +  ++   RMGIG +    + +    VE  R+++A
Sbjct: 384 TVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISIIATLVAGFVEVKRKSVA 443

Query: 444 IKEGFSDDPEAVVSMSAFWTLPRYIFLGMSEGFSAVGQIEFFYFELPRAMSSVATSLFGL 503
           I+ G  D P  +V +S  W +P+Y   G++E F ++G +EFFY + P +M S AT+LF +
Sbjct: 444 IEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQAPESMRSTATALFWM 503

Query: 504 GMSVGNLAASFIMTIVDNFSKTGGGESWV-STNINNGHCDYYYWLLFALVFANFLYFLVC 556
            +S+GN  ++ ++T+V  FS    G +W+   N+N G  +Y+YWL+  L   N +Y+L C
Sbjct: 504 AISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYWLITVLQAVNLVYYLWC 563