MS001209 (gene) Bitter gourd (TR) v1

BLAST of MS001209 vs. NCBI nr
Match: XP_038885421.1 (carotenoid cleavage dioxygenase 8 homolog B, chloroplastic [Benincasa hispida])

HSP 1 Score: 977.6 bits (2526), Expect = 4.3e-281
Identity = 472/546 (86.45%), Postives = 507/546 (92.86%), Query Frame = 0

Query: 1   MASIAAGSCIF-PFNSKTNR--LKRTVFPSPNFTVRSIASPQRHRPPPVAPTTEIDPSGH 60
           MAS+A GS I  PFN KTN    K T+    NFT+RSIASP R   P V P  EID +G 
Sbjct: 2   MASMAIGSSIISPFNFKTNHHPSKPTL---SNFTIRSIASPIRRPSPIVVPLPEIDTTG- 61

Query: 61  RNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYATIVK 120
             NHVAWTS+RQ++WEGELSV+GQLP WLSGTYLRNGPG+WNIGDYNFRHLFDGYATIVK
Sbjct: 62  PPNHVAWTSVRQDRWEGELSVRGQLPRWLSGTYLRNGPGLWNIGDYNFRHLFDGYATIVK 121

Query: 121 LQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASLFSGASLT 180
           L FDDGR+IAGHRQIES+AY AA++NQK+C+REFSEVPKA+NFLAYVGELA+LFSGASLT
Sbjct: 122 LHFDDGRLIAGHRQIESDAYKAAMKNQKICYREFSEVPKADNFLAYVGELANLFSGASLT 181

Query: 181 DNSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEYSDSLGGLIHSAHPIVTDSE 240
           DN+NTGV+KLGDGRVVCLTETQKGSIMID  +LET+GKFEYSDSLGGLIHSAHPIVTD+E
Sbjct: 182 DNANTGVIKLGDGRVVCLTETQKGSIMIDPTTLETVGKFEYSDSLGGLIHSAHPIVTDTE 241

Query: 241 FLTLLPDLFNPGYQVVRMEPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMPLR 300
           FLTLLPDL NPGY VVRMEPG+NERKVIGRVNC+GGPAPGWVHSFPVTENYVVVPEMPLR
Sbjct: 242 FLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPLR 301

Query: 301 YCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFINAYEERDE 360
           YCAQNLLRAEPTPLYKFEWRPESKAF+HVMCKASG IV SVEVPL+ITFHFINAYEERDE
Sbjct: 302 YCAQNLLRAEPTPLYKFEWRPESKAFVHVMCKASGNIVASVEVPLFITFHFINAYEERDE 361

Query: 361 EGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNGTLEA 420
           +GRITAVIADCCEHNANPVILDRLRL+NLRR +LK ALPDARVGRFRIP+DGSG G LEA
Sbjct: 362 KGRITAVIADCCEHNANPVILDRLRLHNLRR-SLKYALPDARVGRFRIPLDGSGYGKLEA 421

Query: 421 ALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNWHEDG 480
           AL+P+EHGRGMDMCSFNPA+LGKKYRYAYACGAKRPCNFPNTLTKIDLV+KKAKNW+EDG
Sbjct: 422 ALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAKRPCNFPNTLTKIDLVKKKAKNWYEDG 481

Query: 481 AIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGLH 540
           AIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGLH
Sbjct: 482 AIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGLH 541

Query: 541 GCWVPK 544
           GCWVPK
Sbjct: 542 GCWVPK 542

BLAST of MS001209 vs. NCBI nr
Match: XP_023546621.1 (carotenoid cleavage dioxygenase 8 homolog B, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 973.0 bits (2514), Expect = 1.1e-279
Identity = 468/547 (85.56%), Postives = 504/547 (92.14%), Query Frame = 0

Query: 1   MASIAAGSCI--FPFNS--KTNRLKRTVFPSPNFTVRSIASPQRHRPPPVAPTTEIDPSG 60
           MAS+AAGSCI   P+N     +  KRTV    NFT+RSIASP R  PP V P  +ID S 
Sbjct: 1   MASMAAGSCINFKPYNPCLPLHPPKRTV---SNFTIRSIASPIRPSPPVVVPLPDIDTS- 60

Query: 61  HRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYATIV 120
               HVAWTSIRQ++WEG+LSVQGQLP WL+GTYLRNGPGIWNIG+YNFRHLFDGYATIV
Sbjct: 61  TPPTHVAWTSIRQDRWEGQLSVQGQLPRWLNGTYLRNGPGIWNIGNYNFRHLFDGYATIV 120

Query: 121 KLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASLFSGASL 180
           KL F+DGR+IAGHRQIES AYMAA++NQKVC+REFSEVPK +NFLAYVGELA+LFSG SL
Sbjct: 121 KLHFEDGRLIAGHRQIESNAYMAAMKNQKVCYREFSEVPKTDNFLAYVGELANLFSGVSL 180

Query: 181 TDNSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEYSDSLGGLIHSAHPIVTDS 240
           TDN+NTGVVKLGDGRVVCLTETQKGSI+I+  +LET+GKFEYSDSLGGLIHSAHPIVTDS
Sbjct: 181 TDNANTGVVKLGDGRVVCLTETQKGSIIINPSTLETIGKFEYSDSLGGLIHSAHPIVTDS 240

Query: 241 EFLTLLPDLFNPGYQVVRMEPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMPL 300
           EFLTLLPDL NPGY VVRMEPG+NERKVIGRVNC+GGPAPGWVHSFPVTENYVVVPEMPL
Sbjct: 241 EFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPL 300

Query: 301 RYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFINAYEERD 360
           RYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASG IV SVEVPL+ITFHFINAYEERD
Sbjct: 301 RYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGSIVASVEVPLFITFHFINAYEERD 360

Query: 361 EEGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNGTLE 420
           E+GRITAVIADCCEHNANP+ILDRLRL+NLRRF  K ALPDARVGRFRIP+DGSG+G LE
Sbjct: 361 EKGRITAVIADCCEHNANPLILDRLRLHNLRRFTPKHALPDARVGRFRIPLDGSGHGKLE 420

Query: 421 AALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNWHED 480
           AAL+P+EHGRGMDMCSFNPA+LGKKYRYAYACGAKRPCNFPNTLTKIDLV+KKAKNW+E 
Sbjct: 421 AALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAKRPCNFPNTLTKIDLVKKKAKNWYES 480

Query: 481 GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGL 540
           GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGL
Sbjct: 481 GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGL 540

Query: 541 HGCWVPK 544
           HGCW+PK
Sbjct: 541 HGCWIPK 543

BLAST of MS001209 vs. NCBI nr
Match: KAG6598778.1 (Carotenoid cleavage dioxygenase 8-like B, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 970.7 bits (2508), Expect = 5.3e-279
Identity = 466/547 (85.19%), Postives = 504/547 (92.14%), Query Frame = 0

Query: 1   MASIAAGSCIF--PFNS--KTNRLKRTVFPSPNFTVRSIASPQRHRPPPVAPTTEIDPSG 60
           MAS+AAGSCI   P+N     +  KRTV    NFT+RSIASP R  PP V P  +ID S 
Sbjct: 1   MASMAAGSCISFKPYNPCLPFHPPKRTV---SNFTIRSIASPIRPSPPVVVPLPDIDTS- 60

Query: 61  HRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYATIV 120
               HVAWTSIRQ++WEG+LSVQGQLP WL+GTYLRNGPG+WNIG+YNFRHLFDGYATIV
Sbjct: 61  IPPTHVAWTSIRQDRWEGQLSVQGQLPRWLNGTYLRNGPGVWNIGNYNFRHLFDGYATIV 120

Query: 121 KLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASLFSGASL 180
           KL F+DGR+IAGHRQIES AY AA++NQKVC+REFSEVPK +NFLAYVGELA+LFSG SL
Sbjct: 121 KLHFEDGRLIAGHRQIESNAYKAAMKNQKVCYREFSEVPKTDNFLAYVGELANLFSGVSL 180

Query: 181 TDNSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEYSDSLGGLIHSAHPIVTDS 240
           TDN+NTGVVKLGD RVVCLTETQKGSI+I+  +LET+GKFEYSDSLGGLIHSAHPIVTDS
Sbjct: 181 TDNANTGVVKLGDSRVVCLTETQKGSIIINPSTLETIGKFEYSDSLGGLIHSAHPIVTDS 240

Query: 241 EFLTLLPDLFNPGYQVVRMEPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMPL 300
           EFLTLLPDL NPGY VVRMEPG+NERKVIGRVNC+GGPAPGWVHSFPVTENYVVVPEMPL
Sbjct: 241 EFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPL 300

Query: 301 RYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFINAYEERD 360
           RYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASG IV SVEVPL+ITFHFINAYEERD
Sbjct: 301 RYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGSIVASVEVPLFITFHFINAYEERD 360

Query: 361 EEGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNGTLE 420
           E+GRITAVIADCCEHNANP+ILDRLRL+NLRRF+LK ALPDARVGRFRIP+DGSG+G LE
Sbjct: 361 EKGRITAVIADCCEHNANPLILDRLRLHNLRRFSLKHALPDARVGRFRIPLDGSGHGKLE 420

Query: 421 AALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNWHED 480
           AAL+P+EHGRGMDMCSFNPA+LGKKYRYAYACGAKRPCNFPNTLTKIDLV+KKAKNW+E 
Sbjct: 421 AALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAKRPCNFPNTLTKIDLVKKKAKNWYES 480

Query: 481 GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGL 540
           GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGL
Sbjct: 481 GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGL 540

Query: 541 HGCWVPK 544
           HGCW+PK
Sbjct: 541 HGCWIPK 543

BLAST of MS001209 vs. NCBI nr
Match: XP_023538517.1 (carotenoid cleavage dioxygenase 8 homolog B, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 968.0 bits (2501), Expect = 3.4e-278
Identity = 466/548 (85.04%), Postives = 508/548 (92.70%), Query Frame = 0

Query: 1   MASIAAGSCIFPFNSKT-NRLKRTVFPSPNFTVRSIASPQRHRPPPV-APTTEIDPSGHR 60
           MAS+AAGSC+  FN K+ +  KRTVF   NFT+RSIA+P   RPP V  P  EID   H 
Sbjct: 1   MASMAAGSCMSSFNFKSIHPPKRTVF---NFTIRSIATP--IRPPSVGVPLPEIDTPAH- 60

Query: 61  NNHVAWTSIRQEKWEGELSVQGQLPYWL---SGTYLRNGPGIWNIGDYNFRHLFDGYATI 120
            +HVAWTS++QE+WEGELSVQG+LP WL   +GTYLRNGPGIW+IGDY+FRHLFDGYATI
Sbjct: 61  PSHVAWTSVQQERWEGELSVQGELPRWLVRFNGTYLRNGPGIWHIGDYDFRHLFDGYATI 120

Query: 121 VKLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASLFSGAS 180
           VKLQFDDGR+IAGHRQIES+AY AA++NQK+C+REFSEVPKANNFLAYVGELASLFSGAS
Sbjct: 121 VKLQFDDGRLIAGHRQIESDAYKAAMKNQKICYREFSEVPKANNFLAYVGELASLFSGAS 180

Query: 181 LTDNSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEYSDSLGGLIHSAHPIVTD 240
           LTDN+NTGVVKLGDGRV+CLTETQKGSIMI+  +LET+GKFEYSDSLGGLIHSAHPIVTD
Sbjct: 181 LTDNANTGVVKLGDGRVICLTETQKGSIMINPTTLETVGKFEYSDSLGGLIHSAHPIVTD 240

Query: 241 SEFLTLLPDLFNPGYQVVRMEPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMP 300
           SEFLTLLPDL NPGY VVRMEPG+NERKVIGRVNC+GGPAPGWVHSFPVTENYVVVPEMP
Sbjct: 241 SEFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMP 300

Query: 301 LRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFINAYEER 360
           LRYCAQNLLRAEPTPLYKFEWRPESKA++HVMCKASGKIV SVEVPLYITFHFINAYEER
Sbjct: 301 LRYCAQNLLRAEPTPLYKFEWRPESKAYVHVMCKASGKIVASVEVPLYITFHFINAYEER 360

Query: 361 DEEGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNGTL 420
           DE+GRITAVIADCCEHNANP+ILDRLRL+NLRRF   DALPDAR+GRFRIP+DGSG G L
Sbjct: 361 DEKGRITAVIADCCEHNANPLILDRLRLHNLRRFTPNDALPDARLGRFRIPLDGSGYGEL 420

Query: 421 EAALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNWHE 480
           EAAL+PEEHGRG+DMCSFNPA+LGKKY YAYACGA+RPCNFPNTLTKIDLV+K AKNWHE
Sbjct: 421 EAALDPEEHGRGLDMCSFNPAYLGKKYGYAYACGAQRPCNFPNTLTKIDLVKKTAKNWHE 480

Query: 481 DGAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYG 540
            GAIPSEPFFVARPGATEEDDGVVISMVSG++GEGYALLLDGSTFEEIARAKFPYGLPYG
Sbjct: 481 TGAIPSEPFFVARPGATEEDDGVVISMVSGEDGEGYALLLDGSTFEEIARAKFPYGLPYG 540

Query: 541 LHGCWVPK 544
           LHGCW+PK
Sbjct: 541 LHGCWIPK 542

BLAST of MS001209 vs. NCBI nr
Match: XP_022997653.1 (carotenoid cleavage dioxygenase 8 homolog B, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 967.6 bits (2500), Expect = 4.5e-278
Identity = 465/548 (84.85%), Postives = 503/548 (91.79%), Query Frame = 0

Query: 1   MASIAAGSCIFPFNSKTNRL-----KRTVFPSPNFTVRSIASPQRHRPPPVAPTTEIDPS 60
           MAS+AAGSCI  F +    L     KR+V    NFT+RSIASP R  PP V P   ID S
Sbjct: 1   MASMAAGSCI-SFKAYNPCLPLHPPKRSV---SNFTIRSIASPIRPSPPVVVPFPNIDTS 60

Query: 61  GHRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYATI 120
                HVAWTSIRQ++WEG+LS+QGQLP WL+GTYLRNGPG+WNIG+YNFRHLFDGYATI
Sbjct: 61  -TPPTHVAWTSIRQDQWEGQLSIQGQLPRWLNGTYLRNGPGVWNIGNYNFRHLFDGYATI 120

Query: 121 VKLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASLFSGAS 180
           VKLQF+DGR+IAGHRQIES AY AA++NQKVC+REFSEVPK +NFLAYVGELA+LFSG S
Sbjct: 121 VKLQFEDGRLIAGHRQIESNAYKAAMKNQKVCYREFSEVPKTDNFLAYVGELANLFSGVS 180

Query: 181 LTDNSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEYSDSLGGLIHSAHPIVTD 240
           LTDN+NTGVVKLGD RVVCLTETQKGSI+I+  +LET+GKFEYSDSLGGLIHSAHPIVTD
Sbjct: 181 LTDNANTGVVKLGDSRVVCLTETQKGSIIINPSTLETMGKFEYSDSLGGLIHSAHPIVTD 240

Query: 241 SEFLTLLPDLFNPGYQVVRMEPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMP 300
           SEFLTLLPDL NPGY VVRMEPG+NERKVIGRVNC+GGPAPGWVHSFPVTENYVVVPEMP
Sbjct: 241 SEFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMP 300

Query: 301 LRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFINAYEER 360
           LRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASG IV SVEVPL+ITFHFINAYEER
Sbjct: 301 LRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAYEER 360

Query: 361 DEEGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNGTL 420
           DE+GRITAVIADCCEHNANP+ILDRLRL+NLRRF+LK ALPDARVGRFRIP+DGSG+G L
Sbjct: 361 DEKGRITAVIADCCEHNANPLILDRLRLHNLRRFSLKHALPDARVGRFRIPLDGSGHGKL 420

Query: 421 EAALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNWHE 480
           EAAL+P+EHGRGMDMCSFNPA+LGKKYRYAYACGAKRPCNFPNTLTKIDLV+KKAKNW+E
Sbjct: 421 EAALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAKRPCNFPNTLTKIDLVKKKAKNWYE 480

Query: 481 DGAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYG 540
            GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYG
Sbjct: 481 SGAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYG 540

Query: 541 LHGCWVPK 544
           LHGCW+PK
Sbjct: 541 LHGCWIPK 543

BLAST of MS001209 vs. ExPASy Swiss-Prot
Match: Q8LIY8 (Carotenoid cleavage dioxygenase 8 homolog B, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CCD8B PE=1 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 1.2e-236
Identity = 383/536 (71.46%), Postives = 443/536 (82.65%), Query Frame = 0

Query: 15  SKTNRLKRTVF-PSPNFTVRSIASPQRHRPPPVAPTTEIDPSGHRNNH------VAWTSI 74
           S+ ++ KR V  P     V   A P     PP  P       G R         VAW S+
Sbjct: 29  SQGHQGKRAVAQPLAASAVTEAAPPAPVVAPPARPVDAPRRRGGRGGGGGGGELVAWKSV 88

Query: 75  RQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYATIVKLQFDDGRVIA 134
           RQE+WEG L V G+LP WL GTYLRNGPG+WN+GDY FRHLFDGYAT+V++ F  GR + 
Sbjct: 89  RQERWEGALEVDGELPLWLDGTYLRNGPGLWNLGDYGFRHLFDGYATLVRVSFRGGRAVG 148

Query: 135 GHRQIESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASLFSGASLTDNSNTGVVKL 194
            HRQIESEAY AA  + KVC+REFSEVPK +NFL+YVG+LA+LFSG+SLTDNSNTGVV L
Sbjct: 149 AHRQIESEAYKAARAHGKVCYREFSEVPKPDNFLSYVGQLATLFSGSSLTDNSNTGVVML 208

Query: 195 GDGRVVCLTETQKGSIMIDSDSLETLGKFEYSDSLGGLIHSAHPIVTDSEFLTLLPDLFN 254
           GDGRV+CLTET KGSI +D D+L+T+GKF+Y+D LGGLIHSAHPIVTD+EF TL+PDL  
Sbjct: 209 GDGRVLCLTETIKGSIQVDPDTLDTVGKFQYTDKLGGLIHSAHPIVTDTEFWTLIPDLIR 268

Query: 255 PGYQVVRMEPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMPLRYCAQNLLRAE 314
           PGY V RM+ G+NER+ +GRV+CRGGPAPGWVHSFPVTE+YVVVPEMPLRYCA+NLLRAE
Sbjct: 269 PGYVVARMDAGSNERQFVGRVDCRGGPAPGWVHSFPVTEHYVVVPEMPLRYCAKNLLRAE 328

Query: 315 PTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFINAYEERDEEGRITAVIAD 374
           PTPLYKFEW  ES ++MHVMCKASGKIV SVEVP ++TFHFINAYEE DEEGR+TA+IAD
Sbjct: 329 PTPLYKFEWHLESGSYMHVMCKASGKIVASVEVPPFVTFHFINAYEETDEEGRVTAIIAD 388

Query: 375 CCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNGTLEAALEPEEHGRG 434
           CCEHNAN  ILD+LRL+NLR  + +D LPDARVGRFRIP+DGS  G LE AL+PEEHGRG
Sbjct: 389 CCEHNANTAILDKLRLHNLRSSSGQDVLPDARVGRFRIPLDGSQFGELETALDPEEHGRG 448

Query: 435 MDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNWHEDGAIPSEPFFVA 494
           MDMCS NPA +G++YRYAYACGA+RPCNFPNTLTK+DLVE+ AKNWHE+G++PSEPFFV 
Sbjct: 449 MDMCSINPAHVGREYRYAYACGARRPCNFPNTLTKVDLVERTAKNWHEEGSVPSEPFFVP 508

Query: 495 RPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGLHGCWVPK 544
           RPGATEEDDGV ISMVS K+G GYAL+LDG TFEE+ARAKFPYGLPYGLH CWVP+
Sbjct: 509 RPGATEEDDGVAISMVSAKDGSGYALVLDGKTFEEVARAKFPYGLPYGLHCCWVPR 564

BLAST of MS001209 vs. ExPASy Swiss-Prot
Match: Q8VY26 (Carotenoid cleavage dioxygenase 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CCD8 PE=1 SV=1)

HSP 1 Score: 746.5 bits (1926), Expect = 2.1e-214
Identity = 343/508 (67.52%), Postives = 418/508 (82.28%), Query Frame = 0

Query: 43  PPPVAPTTEIDPSGHRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGD 102
           P P+    +++  G R  HVAWTS++QE WEGEL+VQG++P WL+GTYLRNGPG+WNIGD
Sbjct: 64  PSPLPEKEKVE--GERRCHVAWTSVQQENWEGELTVQGKIPTWLNGTYLRNGPGLWNIGD 123

Query: 103 YNFRHLFDGYATIVKLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFSEVPKA----N 162
           ++FRHLFDGY+T+VKLQFD GR+ A HR +ES+AY AA ++ ++C+REFSE PK+     
Sbjct: 124 HDFRHLFDGYSTLVKLQFDGGRIFAAHRLLESDAYKAAKKHNRLCYREFSETPKSVIINK 183

Query: 163 NFLAYVGELASLFSGASLTDNSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEY 222
           N  + +GE+  LFSG SLTDN+NTGV+KLGDGRV+CLTETQKGSI++D ++LET+GKFEY
Sbjct: 184 NPFSGIGEIVRLFSGESLTDNANTGVIKLGDGRVMCLTETQKGSILVDHETLETIGKFEY 243

Query: 223 SDSLGG-LIHSAHPIVTDSEFLTLLPDLFNPGYQVVRMEPGTNERKVIGRVNCRGGP-AP 282
            D L   +I SAHPIVT++E  TL+PDL  PGY+VVRME G+N+R+V+GRV CR G   P
Sbjct: 244 DDVLSDHMIQSAHPIVTETEMWTLIPDLVKPGYRVVRMEAGSNKREVVGRVRCRSGSWGP 303

Query: 283 GWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVT 342
           GWVHSF VTENYVV+PEMPLRY  +NLLRAEPTPLYKFEW P+  AF+HVM K +G++V 
Sbjct: 304 GWVHSFAVTENYVVIPEMPLRYSVKNLLRAEPTPLYKFEWCPQDGAFIHVMSKLTGEVVA 363

Query: 343 SVEVPLYITFHFINAYEE-RDEEGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDAL 402
           SVEVP Y+TFHFINAYEE ++ +G+ T +IADCCEHNA+  ILD LRL+ LR     D L
Sbjct: 364 SVEVPAYVTFHFINAYEEDKNGDGKATVIIADCCEHNADTRILDMLRLDTLRSSHGHDVL 423

Query: 403 PDARVGRFRIPMDGSGNGTLEAALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCN 462
           PDAR+GRFRIP+DGS  G LE A+E E+HGR MDMCS NP +LG+KYRY YACGA+RPCN
Sbjct: 424 PDARIGRFRIPLDGSKYGKLETAVEAEKHGRAMDMCSINPLYLGQKYRYVYACGAQRPCN 483

Query: 463 FPNTLTKIDLVEKKAKNWHEDGAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLL 522
           FPN L+K+D+VEKK KNWHE G IPSEPFFV RPGAT EDDGVVIS+VS +NG  +A+LL
Sbjct: 484 FPNALSKVDIVEKKVKNWHEHGMIPSEPFFVPRPGATHEDDGVVISIVSEENGGSFAILL 543

Query: 523 DGSTFEEIARAKFPYGLPYGLHGCWVPK 544
           DGS+FEEIARAKFPYGLPYGLHGCW+PK
Sbjct: 544 DGSSFEEIARAKFPYGLPYGLHGCWIPK 569

BLAST of MS001209 vs. ExPASy Swiss-Prot
Match: Q93VD5 (Carotenoid cleavage dioxygenase 8 homolog A, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CCD8A PE=2 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 1.6e-198
Identity = 320/486 (65.84%), Postives = 390/486 (80.25%), Query Frame = 0

Query: 63  AWTSIRQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYATIVKLQFDD 122
           A TS+RQE+WEG+L ++G LP WL+GTY+RNGPG+W++G++ F HLFDGYAT+V++ F  
Sbjct: 68  ASTSVRQERWEGDLPIEGCLPPWLNGTYIRNGPGMWDVGEHAFHHLFDGYATLVRVSFRG 127

Query: 123 G---RVIAGHRQIESEAYMAAVRNQKVCFREFSEVP-KANNFLAYVGELASLFSGASLTD 182
           G   R    HRQIESEAY AAV   +   REFS  P  A + L   G+L  L +GA+LTD
Sbjct: 128 GGGARATGAHRQIESEAYRAAVARGRPVLREFSHCPAPAKSLLHRFGDLVGLVTGAALTD 187

Query: 183 NSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEYSDSLGGLIHSAHPIVTDSEF 242
           N N+ V+ LGDGRV+CLTET K S++ID D+LET+G+F Y+D LGG++ SAHPIVTD+EF
Sbjct: 188 NPNSAVLPLGDGRVMCLTETTKSSVLIDPDTLETVGRFRYTDRLGGMVQSAHPIVTDTEF 247

Query: 243 LTLLPDLFNPGYQVVRMEPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMPLRY 302
           LTLLPDL  PG+ VVRME G+NERKVIGR++CRGGP+PGW+HSF VTE Y VVPEMPLRY
Sbjct: 248 LTLLPDLVRPGHLVVRMEAGSNERKVIGRMDCRGGPSPGWLHSFAVTEKYAVVPEMPLRY 307

Query: 303 CAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFINAYEERDEE 362
            + +LL +E  P Y F+W P S ++MHVMCK++GK V SVEVP ++  HFINAYE   EE
Sbjct: 308 SSASLLASELAPFYAFDWVPASGSYMHVMCKSTGKTVASVEVPPFMAIHFINAYE---EE 367

Query: 363 GRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNGTLEAA 422
           G   AV+ DCCEH  +P I++ L L+ LR    KD LP+ARVGRFRIP+DGS  G LE A
Sbjct: 368 GDEAAVVVDCCEHYGDPAIIETLVLSRLRLLRGKDVLPNARVGRFRIPLDGSPFGELETA 427

Query: 423 LEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNWHEDGA 482
           L+PEEHGRGMDMCS NPA LG+KY+YAYACGA+RPCNFPNTLTKIDLVEKKAK+WHE+G+
Sbjct: 428 LDPEEHGRGMDMCSINPARLGRKYQYAYACGARRPCNFPNTLTKIDLVEKKAKSWHEEGS 487

Query: 483 IPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGLHG 542
           +PSEPFFVARPGAT+EDDGVVIS+VS  +GEGYAL+LD +TFEEIAR +FPYGLPYG HG
Sbjct: 488 VPSEPFFVARPGATDEDDGVVISIVSSDDGEGYALVLDATTFEEIARVRFPYGLPYGFHG 547

Query: 543 CWVPKT 545
           CW+P T
Sbjct: 548 CWIPAT 550

BLAST of MS001209 vs. ExPASy Swiss-Prot
Match: Q8HXG8 (Beta,beta-carotene 9',10'-oxygenase OS=Macaca fascicularis OX=9541 GN=BCO2 PE=2 SV=2)

HSP 1 Score: 202.2 bits (513), Expect = 1.5e-50
Identity = 148/521 (28.41%), Postives = 235/521 (45.11%), Query Frame = 0

Query: 78  VQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYATIVKLQFDDGRVIAGHRQIESEAY 137
           V G  P WL+G+ LR GPG +  G   + H FDG A + + +   G V    + ++S+ Y
Sbjct: 49  VWGHFPKWLNGSLLRIGPGKFEFGKDKYNHWFDGMALLHQFRMAKGTVTYRSKFLQSDTY 108

Query: 138 MAAVRNQKVCFREFSEV----PKANNFLAYVGELASLFSGASLTDNSNTGVVKLGDGRVV 197
            A     ++   EF  +    P  N F  ++         A++TDN+N   V+      +
Sbjct: 109 KANSAKNRIVMSEFGTLATPDPCKNVFERFMSRFELPGKAAAMTDNTNVNYVRYKGDYYL 168

Query: 198 CLTETQKGSIMIDSDSLETLGKFEYSDSLGGLIHSAHPIVTDSEFLTLLPDLFNP---GY 257
           C TET   +  +D ++LE   K ++S  +     +AHP          + + F P    Y
Sbjct: 169 C-TETNFMN-KVDIETLEKTEKVDWSKFIAVNGATAHPHYDPDGTAYNMGNSFGPFGFSY 228

Query: 258 QVVRMEP-------GTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMPLRYCAQNL 317
           +V+R+ P        T+  +VI  +       P + HSF +T NY++  E PL+    NL
Sbjct: 229 KVIRVPPEKVDLEETTHGAQVICSIAPTEKGKPSYYHSFGMTRNYIIFIEQPLK---MNL 288

Query: 318 LRAEPTPLY------KFEWRPESKAFMHVMCKASGKIVTS-VEVPLYITFHFINAYEERD 377
            +   + +          W P+     HV+ K +G+++        ++ FH INA+E++ 
Sbjct: 289 WKIATSKIRGKAFSDGISWEPQCNTRFHVVDKHTGQLLPGRYYSKPFVAFHHINAFEDQG 348

Query: 378 EEGRITAVIADCCEHNANPVILDRLRLNNLRRFA-----LKDALPDARVGRFRIPMDGSG 437
                  +I  CC+ N    IL+  +L NLR+       + ++   +   RF +P++ S 
Sbjct: 349 -----CVIIDLCCQDNGR--ILEVYQLQNLRKAGEELDQVYNSAGRSFPRRFVLPLNVSL 408

Query: 438 NGTLEAALEP--------EEHGRGMDMCS--------------------FNPAFLGKKYR 497
           N      L P         +   G   CS                    +   F GKKYR
Sbjct: 409 NAPEGDNLSPLSYTSASAVKQADGTIWCSHENLHQEDLEKEGGIEFPQIYYGQFSGKKYR 468

Query: 498 YAYACGAKRPCNFPNTLTKIDLVEKKAKNWHEDGAIPSEPFFVARPGATEEDDGVVISMV 543
           + Y CG +      ++L K+D+V K  K W EDG  PSEP FV  PG  EED GV++S+V
Sbjct: 469 FFYGCGFRHLVG--DSLIKVDVVNKTLKVWREDGFYPSEPVFVPVPGTNEEDGGVILSVV 528

BLAST of MS001209 vs. ExPASy Swiss-Prot
Match: Q9BYV7 (Beta,beta-carotene 9',10'-oxygenase OS=Homo sapiens OX=9606 GN=BCO2 PE=1 SV=5)

HSP 1 Score: 198.7 bits (504), Expect = 1.6e-49
Identity = 146/521 (28.02%), Postives = 233/521 (44.72%), Query Frame = 0

Query: 78  VQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYATIVKLQFDDGRVIAGHRQIESEAY 137
           V G  P WL+G+ LR GPG +  G   + H FDG A + + +   G V    + ++S+ Y
Sbjct: 72  VWGHFPKWLNGSLLRIGPGKFEFGKDKYNHWFDGMALLHQFRMAKGTVTYRSKFLQSDTY 131

Query: 138 MAAVRNQKVCFREFSEV----PKANNFLAYVGELASLFSGASLTDNSNTGVVKLGDGRVV 197
            A     ++   EF  +    P  N F  ++         A++TDN+N   V+      +
Sbjct: 132 KANSAKNRIVISEFGTLALPDPCKNVFERFMSRFELPGKAAAMTDNTNVNYVRYKGDYYL 191

Query: 198 CLTETQKGSIMIDSDSLETLGKFEYSDSLGGLIHSAHPIVTDSEFLTLLPDLFNP---GY 257
           C TET   +  +D ++LE   K ++S  +     +AHP          + + F P    Y
Sbjct: 192 C-TETNFMN-KVDIETLEKTEKVDWSKFIAVNGATAHPHYDLDGTAYNMGNSFGPYGFSY 251

Query: 258 QVVRMEPGTNER-------KVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMPLRYCAQNL 317
           +V+R+ P   +        +VI  +       P + HSF +T NY++  E PL+    NL
Sbjct: 252 KVIRVPPEKVDLGETIHGVQVICSIASTEKGKPSYYHSFGMTRNYIIFIEQPLK---MNL 311

Query: 318 LRAEPTPLY------KFEWRPESKAFMHVMCKASGKIVTS-VEVPLYITFHFINAYEERD 377
            +   + +          W P+     HV+ K +G+++        ++TFH INA+E++ 
Sbjct: 312 WKIATSKIRGKAFSDGISWEPQCNTRFHVVEKRTGQLLPGRYYSKPFVTFHQINAFEDQG 371

Query: 378 EEGRITAVIADCCEHNANPVILDRLRLNNLRRFA-----LKDALPDARVGRFRIPMDGSG 437
                  +I  CC+ N     L+  +L NLR+       + ++   +   RF +P++ S 
Sbjct: 372 -----CVIIDLCCQDNGR--TLEVYQLQNLRKAGEGLDQVHNSAAKSFPRRFVLPLNVSL 431

Query: 438 NGTLEAALEP--------EEHGRGMDMCS--------------------FNPAFLGKKYR 497
           N      L P         +   G   CS                    +   F GKKY 
Sbjct: 432 NAPEGDNLSPLSYTSASAVKQADGTIWCSHENLHQEDLEKEGGIEFPQIYYDRFSGKKYH 491

Query: 498 YAYACGAKRPCNFPNTLTKIDLVEKKAKNWHEDGAIPSEPFFVARPGATEEDDGVVISMV 543
           + Y CG +      ++L K+D+V K  K W EDG  PSEP FV  PG  EED GV++S+V
Sbjct: 492 FFYGCGFRHLVG--DSLIKVDVVNKTLKVWREDGFYPSEPVFVPAPGTNEEDGGVILSVV 551

BLAST of MS001209 vs. ExPASy TrEMBL
Match: A0A6J1KEJ8 (carotenoid cleavage dioxygenase 8 homolog B, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111492518 PE=3 SV=1)

HSP 1 Score: 967.6 bits (2500), Expect = 2.2e-278
Identity = 465/548 (84.85%), Postives = 503/548 (91.79%), Query Frame = 0

Query: 1   MASIAAGSCIFPFNSKTNRL-----KRTVFPSPNFTVRSIASPQRHRPPPVAPTTEIDPS 60
           MAS+AAGSCI  F +    L     KR+V    NFT+RSIASP R  PP V P   ID S
Sbjct: 1   MASMAAGSCI-SFKAYNPCLPLHPPKRSV---SNFTIRSIASPIRPSPPVVVPFPNIDTS 60

Query: 61  GHRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYATI 120
                HVAWTSIRQ++WEG+LS+QGQLP WL+GTYLRNGPG+WNIG+YNFRHLFDGYATI
Sbjct: 61  -TPPTHVAWTSIRQDQWEGQLSIQGQLPRWLNGTYLRNGPGVWNIGNYNFRHLFDGYATI 120

Query: 121 VKLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASLFSGAS 180
           VKLQF+DGR+IAGHRQIES AY AA++NQKVC+REFSEVPK +NFLAYVGELA+LFSG S
Sbjct: 121 VKLQFEDGRLIAGHRQIESNAYKAAMKNQKVCYREFSEVPKTDNFLAYVGELANLFSGVS 180

Query: 181 LTDNSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEYSDSLGGLIHSAHPIVTD 240
           LTDN+NTGVVKLGD RVVCLTETQKGSI+I+  +LET+GKFEYSDSLGGLIHSAHPIVTD
Sbjct: 181 LTDNANTGVVKLGDSRVVCLTETQKGSIIINPSTLETMGKFEYSDSLGGLIHSAHPIVTD 240

Query: 241 SEFLTLLPDLFNPGYQVVRMEPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMP 300
           SEFLTLLPDL NPGY VVRMEPG+NERKVIGRVNC+GGPAPGWVHSFPVTENYVVVPEMP
Sbjct: 241 SEFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMP 300

Query: 301 LRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFINAYEER 360
           LRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASG IV SVEVPL+ITFHFINAYEER
Sbjct: 301 LRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAYEER 360

Query: 361 DEEGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNGTL 420
           DE+GRITAVIADCCEHNANP+ILDRLRL+NLRRF+LK ALPDARVGRFRIP+DGSG+G L
Sbjct: 361 DEKGRITAVIADCCEHNANPLILDRLRLHNLRRFSLKHALPDARVGRFRIPLDGSGHGKL 420

Query: 421 EAALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNWHE 480
           EAAL+P+EHGRGMDMCSFNPA+LGKKYRYAYACGAKRPCNFPNTLTKIDLV+KKAKNW+E
Sbjct: 421 EAALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAKRPCNFPNTLTKIDLVKKKAKNWYE 480

Query: 481 DGAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYG 540
            GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYG
Sbjct: 481 SGAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYG 540

Query: 541 LHGCWVPK 544
           LHGCW+PK
Sbjct: 541 LHGCWIPK 543

BLAST of MS001209 vs. ExPASy TrEMBL
Match: A0A6J1HGQ6 (carotenoid cleavage dioxygenase 8 homolog B, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111462801 PE=3 SV=1)

HSP 1 Score: 966.8 bits (2498), Expect = 3.7e-278
Identity = 464/547 (84.83%), Postives = 503/547 (91.96%), Query Frame = 0

Query: 1   MASIAAGSCIF--PFNS--KTNRLKRTVFPSPNFTVRSIASPQRHRPPPVAPTTEIDPSG 60
           MAS+AAGSCI   P+N     +  KRTV    +FT+RSIASP R  PP V P  +ID S 
Sbjct: 1   MASMAAGSCISFKPYNPCLPLHPPKRTV---SSFTIRSIASPIRPSPPVVVPLPDIDTS- 60

Query: 61  HRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYATIV 120
               HVAWTSIRQ++WEG+LSVQGQLP WL+GTYLRNGPG+WNIG+YNFRHLFDGYATIV
Sbjct: 61  IPPTHVAWTSIRQDRWEGQLSVQGQLPRWLNGTYLRNGPGVWNIGNYNFRHLFDGYATIV 120

Query: 121 KLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASLFSGASL 180
           KL F+DGR+IAGHRQIES AY AA++NQKVC+REFSEVPK +NFLAYVGELA+LFSG SL
Sbjct: 121 KLHFEDGRLIAGHRQIESNAYKAAMKNQKVCYREFSEVPKTDNFLAYVGELANLFSGVSL 180

Query: 181 TDNSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEYSDSLGGLIHSAHPIVTDS 240
           TDN+NTGVVKLGD RVVCLTETQKGSI+I+  +LET+GKFEYSDSLGGLIHSAHPIVTD 
Sbjct: 181 TDNANTGVVKLGDSRVVCLTETQKGSIIINPSTLETIGKFEYSDSLGGLIHSAHPIVTDL 240

Query: 241 EFLTLLPDLFNPGYQVVRMEPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMPL 300
           EFLTLLPDL NPGY VVRMEPG+NERKVIGRVNC+GGPAPGWVHSFPVTENYVVVPEMPL
Sbjct: 241 EFLTLLPDLLNPGYLVVRMEPGSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEMPL 300

Query: 301 RYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFINAYEERD 360
           RYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASG IV SVEVPL+ITFHFINAYEERD
Sbjct: 301 RYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGSIVASVEVPLFITFHFINAYEERD 360

Query: 361 EEGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNGTLE 420
           E+GRITAVIADCCEHNANP+ILDRLRL+NLRRF+LK ALPDARVGRFRIP+DGSG+G LE
Sbjct: 361 EKGRITAVIADCCEHNANPLILDRLRLHNLRRFSLKHALPDARVGRFRIPLDGSGHGKLE 420

Query: 421 AALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNWHED 480
           AAL+P+EHGRGMDMCSFNPA+LGKKYRYAYACGAKRPCNFPNTLTKIDLV+KKAKNW+E 
Sbjct: 421 AALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAKRPCNFPNTLTKIDLVKKKAKNWYES 480

Query: 481 GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGL 540
           GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGL
Sbjct: 481 GAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPYGL 540

Query: 541 HGCWVPK 544
           HGCW+PK
Sbjct: 541 HGCWIPK 543

BLAST of MS001209 vs. ExPASy TrEMBL
Match: A0A0A0LS17 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G373590 PE=3 SV=1)

HSP 1 Score: 961.1 bits (2483), Expect = 2.0e-276
Identity = 463/549 (84.34%), Postives = 498/549 (90.71%), Query Frame = 0

Query: 1   MASIAAGSCIF-PFNSKTNRLKRTVFPS-----PNFTVRSIASPQRHRPPPVAPTTEIDP 60
           MAS+A GSCI  PFN KT      + PS      NFT+RSIASP     P V P  EID 
Sbjct: 2   MASMAMGSCIISPFNFKTKHPILPIQPSSKPTLSNFTIRSIASPVHPPSPVVVPLPEIDT 61

Query: 61  SGHRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYAT 120
           S   NNHVAWTSIRQ++WEGELS+QG LP WLSGTYLRNGPG+WNIGDYNFRHLFDGYAT
Sbjct: 62  S---NNHVAWTSIRQDRWEGELSIQGHLPSWLSGTYLRNGPGLWNIGDYNFRHLFDGYAT 121

Query: 121 IVKLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASLFSGA 180
           IVKL FD+GR+IAGHRQIES AY AA++NQK+C+REFSEVPKA+NFLAYVGELA+LFSGA
Sbjct: 122 IVKLHFDNGRLIAGHRQIESNAYKAAMKNQKICYREFSEVPKADNFLAYVGELANLFSGA 181

Query: 181 SLTDNSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEYSDSLGGLIHSAHPIVT 240
           SLTDN+NTGVVKLGDGRVVCLTETQKGSIMID D+LET+G+FEYSDSLGGLIHSAHPIVT
Sbjct: 182 SLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPDTLETVGQFEYSDSLGGLIHSAHPIVT 241

Query: 241 DSEFLTLLPDLFNPGYQVVRMEPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPEM 300
           DSEFLTLLPDL NPGY VVRMEP +NERKVIGRVNC+GGPAPGWVHSFPVTENYVVVPEM
Sbjct: 242 DSEFLTLLPDLLNPGYLVVRMEPSSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPEM 301

Query: 301 PLRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFINAYEE 360
           PLRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASG IV SVEVPL+ITFHFINAYEE
Sbjct: 302 PLRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAYEE 361

Query: 361 RDEEGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNGT 420
           RDE+GRITAVIADCCEHNA+P ILDRLRL+NLR  +LK  LPDARVGRFRIP+DGSG G 
Sbjct: 362 RDEKGRITAVIADCCEHNADPAILDRLRLHNLRD-SLKYPLPDARVGRFRIPLDGSGYGK 421

Query: 421 LEAALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNWH 480
           LEAAL+P+EHGRGMDMCSFNPA+LGKKYRYAYACGA+RPCNFPNTLTKIDLV+K AKNW+
Sbjct: 422 LEAALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAERPCNFPNTLTKIDLVKKVAKNWY 481

Query: 481 EDGAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLPY 540
           E+G IPSEPFFVARPGATEEDDGVVISMVS +NGEGYALLLDGSTFEEIARAKFPYGLPY
Sbjct: 482 EEGTIPSEPFFVARPGATEEDDGVVISMVSAQNGEGYALLLDGSTFEEIARAKFPYGLPY 541

Query: 541 GLHGCWVPK 544
           GLHGCWVPK
Sbjct: 542 GLHGCWVPK 546

BLAST of MS001209 vs. ExPASy TrEMBL
Match: A0A5A7VHT5 (Carotenoid cleavage dioxygenase 8-like protein B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003670 PE=3 SV=1)

HSP 1 Score: 958.4 bits (2476), Expect = 1.3e-275
Identity = 465/550 (84.55%), Postives = 499/550 (90.73%), Query Frame = 0

Query: 1   MASIAAGSCIF-PFNSKTNR-LKRTVFPS-----PNFTVRSIASPQRHRPPPVAPTTEID 60
           MAS+A GSCI  PFN KT   L     PS      NFT+RSIASP R   P V P  EID
Sbjct: 2   MASMAIGSCIISPFNFKTKHPLLPIQQPSSKPTLSNFTIRSIASPVRPPSPVVVPLPEID 61

Query: 61  PSGHRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYA 120
            +    NHVAWTSIRQ++WEGELSVQG LP WLSGTYLRNGPG+WNIGDY+FRHLFDGYA
Sbjct: 62  -TFDAPNHVAWTSIRQDRWEGELSVQGHLPSWLSGTYLRNGPGLWNIGDYDFRHLFDGYA 121

Query: 121 TIVKLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASLFSG 180
            IVKL FD+GR+IAGHRQIES AY AA++NQK+C+REFSEVPKA+NFLAYVGELA+LFSG
Sbjct: 122 MIVKLHFDNGRLIAGHRQIESNAYKAAMKNQKICYREFSEVPKADNFLAYVGELANLFSG 181

Query: 181 ASLTDNSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEYSDSLGGLIHSAHPIV 240
           ASLTDN+NTGVVKLGDGRVVCLTETQKGSIMID D+LET+GKFEYSDSLGGLIHSAHPIV
Sbjct: 182 ASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPDTLETVGKFEYSDSLGGLIHSAHPIV 241

Query: 241 TDSEFLTLLPDLFNPGYQVVRMEPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPE 300
           TDSEFLTLLPDL NPGY VVRMEP +NERKVIGRVNC+GGPAPGWVHSFPVTENYVVVPE
Sbjct: 242 TDSEFLTLLPDLLNPGYLVVRMEPSSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPE 301

Query: 301 MPLRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFINAYE 360
           MPLRYCAQNLL+AEPTPLYKFEWRPESKAFMHVMCKASG IV SVEVPL+ITFHFINAYE
Sbjct: 302 MPLRYCAQNLLKAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAYE 361

Query: 361 ERDEEGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNG 420
           ERDE+GRITAVIADCCEHNANPVILDRLRL+NLR  +LK  LPDARVGRFRIP+DGSG G
Sbjct: 362 ERDEKGRITAVIADCCEHNANPVILDRLRLHNLRD-SLKYPLPDARVGRFRIPLDGSGYG 421

Query: 421 TLEAALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNW 480
            LEAAL+P+EHGRGMDMCSFNPA+LGKKYRYAYACGA+RPCNFPNTLTKIDLV+K AKNW
Sbjct: 422 ELEAALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAERPCNFPNTLTKIDLVKKMAKNW 481

Query: 481 HEDGAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLP 540
           +E+G +PSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLP
Sbjct: 482 YEEGTVPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLP 541

Query: 541 YGLHGCWVPK 544
           YGLHGCWVPK
Sbjct: 542 YGLHGCWVPK 549

BLAST of MS001209 vs. ExPASy TrEMBL
Match: A0A1S3BBP5 (carotenoid cleavage dioxygenase 8 homolog B, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=CCD8 PE=3 SV=1)

HSP 1 Score: 958.4 bits (2476), Expect = 1.3e-275
Identity = 465/550 (84.55%), Postives = 499/550 (90.73%), Query Frame = 0

Query: 1   MASIAAGSCIF-PFNSKTNR-LKRTVFPS-----PNFTVRSIASPQRHRPPPVAPTTEID 60
           MAS+A GSCI  PFN KT   L     PS      NFT+RSIASP R   P V P  EID
Sbjct: 2   MASMAIGSCIISPFNFKTKHPLLPIQQPSSKPTLSNFTIRSIASPVRPPSPVVVPLPEID 61

Query: 61  PSGHRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYA 120
            +    NHVAWTSIRQ++WEGELSVQG LP WLSGTYLRNGPG+WNIGDY+FRHLFDGYA
Sbjct: 62  -TFDAPNHVAWTSIRQDRWEGELSVQGHLPSWLSGTYLRNGPGLWNIGDYDFRHLFDGYA 121

Query: 121 TIVKLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASLFSG 180
            IVKL FD+GR+IAGHRQIES AY AA++NQK+C+REFSEVPKA+NFLAYVGELA+LFSG
Sbjct: 122 MIVKLHFDNGRLIAGHRQIESNAYKAAMKNQKICYREFSEVPKADNFLAYVGELANLFSG 181

Query: 181 ASLTDNSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEYSDSLGGLIHSAHPIV 240
           ASLTDN+NTGVVKLGDGRVVCLTETQKGSIMID D+LET+GKFEYSDSLGGLIHSAHPIV
Sbjct: 182 ASLTDNANTGVVKLGDGRVVCLTETQKGSIMIDPDTLETVGKFEYSDSLGGLIHSAHPIV 241

Query: 241 TDSEFLTLLPDLFNPGYQVVRMEPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPE 300
           TDSEFLTLLPDL NPGY VVRMEP +NERKVIGRVNC+GGPAPGWVHSFPVTENYVVVPE
Sbjct: 242 TDSEFLTLLPDLLNPGYLVVRMEPSSNERKVIGRVNCKGGPAPGWVHSFPVTENYVVVPE 301

Query: 301 MPLRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTSVEVPLYITFHFINAYE 360
           MPLRYCAQNLL+AEPTPLYKFEWRPESKAFMHVMCKASG IV SVEVPL+ITFHFINAYE
Sbjct: 302 MPLRYCAQNLLKAEPTPLYKFEWRPESKAFMHVMCKASGNIVASVEVPLFITFHFINAYE 361

Query: 361 ERDEEGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNG 420
           ERDE+GRITAVIADCCEHNANPVILDRLRL+NLR  +LK  LPDARVGRFRIP+DGSG G
Sbjct: 362 ERDEKGRITAVIADCCEHNANPVILDRLRLHNLRD-SLKYPLPDARVGRFRIPLDGSGYG 421

Query: 421 TLEAALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNW 480
            LEAAL+P+EHGRGMDMCSFNPA+LGKKYRYAYACGA+RPCNFPNTLTKIDLV+K AKNW
Sbjct: 422 ELEAALDPDEHGRGMDMCSFNPAYLGKKYRYAYACGAERPCNFPNTLTKIDLVKKMAKNW 481

Query: 481 HEDGAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLP 540
           +E+G +PSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLP
Sbjct: 482 YEEGTVPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLLDGSTFEEIARAKFPYGLP 541

Query: 541 YGLHGCWVPK 544
           YGLHGCWVPK
Sbjct: 542 YGLHGCWVPK 549

BLAST of MS001209 vs. TAIR 10
Match: AT4G32810.1 (carotenoid cleavage dioxygenase 8 )

HSP 1 Score: 746.5 bits (1926), Expect = 1.5e-215
Identity = 343/508 (67.52%), Postives = 418/508 (82.28%), Query Frame = 0

Query: 43  PPPVAPTTEIDPSGHRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRNGPGIWNIGD 102
           P P+    +++  G R  HVAWTS++QE WEGEL+VQG++P WL+GTYLRNGPG+WNIGD
Sbjct: 64  PSPLPEKEKVE--GERRCHVAWTSVQQENWEGELTVQGKIPTWLNGTYLRNGPGLWNIGD 123

Query: 103 YNFRHLFDGYATIVKLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFREFSEVPKA----N 162
           ++FRHLFDGY+T+VKLQFD GR+ A HR +ES+AY AA ++ ++C+REFSE PK+     
Sbjct: 124 HDFRHLFDGYSTLVKLQFDGGRIFAAHRLLESDAYKAAKKHNRLCYREFSETPKSVIINK 183

Query: 163 NFLAYVGELASLFSGASLTDNSNTGVVKLGDGRVVCLTETQKGSIMIDSDSLETLGKFEY 222
           N  + +GE+  LFSG SLTDN+NTGV+KLGDGRV+CLTETQKGSI++D ++LET+GKFEY
Sbjct: 184 NPFSGIGEIVRLFSGESLTDNANTGVIKLGDGRVMCLTETQKGSILVDHETLETIGKFEY 243

Query: 223 SDSLGG-LIHSAHPIVTDSEFLTLLPDLFNPGYQVVRMEPGTNERKVIGRVNCRGGP-AP 282
            D L   +I SAHPIVT++E  TL+PDL  PGY+VVRME G+N+R+V+GRV CR G   P
Sbjct: 244 DDVLSDHMIQSAHPIVTETEMWTLIPDLVKPGYRVVRMEAGSNKREVVGRVRCRSGSWGP 303

Query: 283 GWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVT 342
           GWVHSF VTENYVV+PEMPLRY  +NLLRAEPTPLYKFEW P+  AF+HVM K +G++V 
Sbjct: 304 GWVHSFAVTENYVVIPEMPLRYSVKNLLRAEPTPLYKFEWCPQDGAFIHVMSKLTGEVVA 363

Query: 343 SVEVPLYITFHFINAYEE-RDEEGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDAL 402
           SVEVP Y+TFHFINAYEE ++ +G+ T +IADCCEHNA+  ILD LRL+ LR     D L
Sbjct: 364 SVEVPAYVTFHFINAYEEDKNGDGKATVIIADCCEHNADTRILDMLRLDTLRSSHGHDVL 423

Query: 403 PDARVGRFRIPMDGSGNGTLEAALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCN 462
           PDAR+GRFRIP+DGS  G LE A+E E+HGR MDMCS NP +LG+KYRY YACGA+RPCN
Sbjct: 424 PDARIGRFRIPLDGSKYGKLETAVEAEKHGRAMDMCSINPLYLGQKYRYVYACGAQRPCN 483

Query: 463 FPNTLTKIDLVEKKAKNWHEDGAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYALLL 522
           FPN L+K+D+VEKK KNWHE G IPSEPFFV RPGAT EDDGVVIS+VS +NG  +A+LL
Sbjct: 484 FPNALSKVDIVEKKVKNWHEHGMIPSEPFFVPRPGATHEDDGVVISIVSEENGGSFAILL 543

Query: 523 DGSTFEEIARAKFPYGLPYGLHGCWVPK 544
           DGS+FEEIARAKFPYGLPYGLHGCW+PK
Sbjct: 544 DGSSFEEIARAKFPYGLPYGLHGCWIPK 569

BLAST of MS001209 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 114.8 bits (286), Expect = 2.2e-25
Identity = 133/526 (25.29%), Postives = 218/526 (41.44%), Query Frame = 0

Query: 34  SIASPQRHRPPPVAPTTEIDPSGHRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRN 93
           SI  P     P   PT   DP+   + + A   + +   +  L V GQ+P  L G Y+RN
Sbjct: 80  SIVIPMEQNRPLPKPT---DPAVQLSGNFA--PVNECPVQNGLEVVGQIPSCLKGVYIRN 139

Query: 94  GPGIWNIGDYNFRHLFDGYATI--VKLQFDDG-----RVIAGHRQIESEAYMAAVRNQKV 153
           G            HLFDG   I  V + FD+      R    +R ++  A   +V  + +
Sbjct: 140 GANPM-FPPLAGHHLFDGDGMIHAVSIGFDNQVSYSCRYTKTNRLVQETALGRSVFPKPI 199

Query: 154 C-FREFSEVPKANNFLAYVGELASLFSGASLTDNSNTGVVKLGDGRVVCLTETQ-KGSIM 213
                 S + +   F A  G    L  G      +N GVV   +GR++ ++E      + 
Sbjct: 200 GELHGHSGLARLALFTARAG--IGLVDGTRGMGVANAGVV-FFNGRLLAMSEDDLPYQVK 259

Query: 214 IDSD-SLETLGKFEYSDSLGGLIHSAHPIV--TDSEFLTLLPD-LFNPGYQVVRMEP-GT 273
           ID    LET+G+F + D +   +  AHP V  T  +  TL  + L  P  + ++    G 
Sbjct: 260 IDGQGDLETIGRFGFDDQIDSSV-IAHPKVDATTGDLHTLSYNVLKKPHLRYLKFNTCGK 319

Query: 274 NERKVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPE 333
             R V   +     P P  +H F +TEN+VV+P+  + +    ++R     +Y      E
Sbjct: 320 KTRDVEITL-----PEPTMIHDFAITENFVVIPDQQMVFKLSEMIRGGSPVIYV----KE 379

Query: 334 SKAFMHVMCK--ASGKIVTSVEVPLYITFHFINAYEERDEEGRITAVIADCCEHNANPVI 393
             A   V+ K   +G  +  V+VP    FH  NA+EER EEG    V+   C    + + 
Sbjct: 380 KMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWEERTEEGDPVIVVIGSCMSPPDTI- 439

Query: 394 LDRLRLNNLRRFALKDALPDARVGRFRIPMDGSGNGTLEAALEPEEHGRGMDMCSFNPAF 453
                      F+         +   R+ M      T E+  +    G  ++    N ++
Sbjct: 440 -----------FSESGEPTRVELSEIRLNM-----RTKESNRKVIVTGVNLEAGHINRSY 499

Query: 454 LGKKYRYAYACGAKRPCNFPNTLTKIDLVEKKAKNW-HEDGAIPSEPFFVARPGATEEDD 513
           +G+K ++ Y   A  P    + + K+D+       + +       EP FV   G  EED 
Sbjct: 500 VGRKSQFVYIAIAD-PWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVPE-GEGEEDK 559

Query: 514 GVVISMV-SGKNGEGYALLLDGSTFEEIARAKFPYGLPYGLHGCWV 542
           G V+  V   +  E   +++D +  +++A  + P  +PYG HG +V
Sbjct: 560 GYVMGFVRDEEKDESEFVVVDATDMKQVAAVRLPERVPYGFHGTFV 567

BLAST of MS001209 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 100.1 bits (248), Expect = 5.6e-21
Identity = 117/523 (22.37%), Postives = 212/523 (40.54%), Query Frame = 0

Query: 35  IASPQRHRPPPVAPTTEIDPSGHRNNHVAWTSIRQEKWEGELSVQGQLPYWLSGTYLRNG 94
           + S ++  P P      +  +G+      +  + ++     L V G+LP  + G Y+RNG
Sbjct: 104 LVSHEKLHPLPKTADPSVQIAGN------FAPVNEQPVRRNLPVVGKLPDSIKGVYVRNG 163

Query: 95  PGIWNIGDYNFRHLFDGYATIVKLQFDDGRVIAGHRQIESEAYMAAVRNQKVCFRE---- 154
               +       H FDG   +  ++F+ G      R  ++  ++   +  +  F +    
Sbjct: 164 ANPLH-EPVTGHHFFDGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGE 223

Query: 155 -FSEVPKANNFLAYVGELASLFSGASLTDNSNTGVVKLGDGRVVCLTETQ---KGSIMID 214
                  A   L Y    A +   A  T  +N G+V   +GR++ ++E     +  I  +
Sbjct: 224 LHGHTGIARLMLFYARAAAGIVDPAHGTGVANAGLVYF-NGRLLAMSEDDLPYQVQITPN 283

Query: 215 SDSLETLGKFEYSDSLGGLIHSAHPIV--TDSEFLTLLPDLFNPGY-QVVRMEP-GTNER 274
            D L+T+G+F++   L   +  AHP V     E   L  D+ +  Y +  R  P GT   
Sbjct: 284 GD-LKTVGRFDFDGQLESTM-IAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSP 343

Query: 275 KVIGRVNCRGGPAPGWVHSFPVTENYVVVPEMPLRYCAQNLLRAEPTPLYKFEWRPESKA 334
            V  +++      P  +H F +TEN+VVVP+  + +    ++R     +Y         A
Sbjct: 344 DVEIQLD-----QPTMMHDFAITENFVVVPDQQVVFKLPEMIRGGSPVVYD----KNKVA 403

Query: 335 FMHVMCK--ASGKIVTSVEVPLYITFHFINAYEERDEEGRITAVIADCCEHNANPVILDR 394
              ++ K       +  ++ P    FH  NA+EE + +     V+   C    + +    
Sbjct: 404 RFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETD---EVVVIGSCMTPPDSIF--- 463

Query: 395 LRLNNLRRFALKDALPDARVGRFRIPMDGSGNGTLEAALEPEEHGRGMDMCSFNPAFLGK 454
               N     LK  L + R+         +G  T    +  E+    ++    N   LG+
Sbjct: 464 ----NESDENLKSVLSEIRLNL------KTGESTRRPIISNEDQQVNLEAGMVNRNMLGR 523

Query: 455 KYRYAYACGAKRPCNFPNTLTKIDLVEKKA-KNWHEDGAIPSEPFFVARPGATEEDDGVV 514
           K ++AY   A+ P    +   K+DL   +  K+ + D     EP F+   G  EED+G +
Sbjct: 524 KTKFAYLALAE-PWPKVSGFAKVDLTTGEVKKHLYGDNRYGGEPLFLPGEGG-EEDEGYI 583

Query: 515 ISMV-SGKNGEGYALLLDGSTFEEIARAKFPYGLPYGLHGCWV 542
           +  V   K  +    +++  + E  A  K P  +PYG HG ++
Sbjct: 584 LCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRVPYGFHGTFI 589

BLAST of MS001209 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 89.0 bits (219), Expect = 1.3e-17
Identity = 122/556 (21.94%), Postives = 215/556 (38.67%), Query Frame = 0

Query: 14  NSKTNRLKRTVFPSPNFTVRSIASPQRHR-PPPVAPTTEIDPSGHRNNHVAWTSIRQEKW 73
           N K N ++RT     +    ++ S +R R P P      +  +G+      +  + ++  
Sbjct: 142 NPKLNPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQIAGN------FFPVPEKPV 201

Query: 74  EGELSVQGQLPYWLSGTYLRNGPGIWNIGDYNFRHLFDGYATIVKLQFDDGRVIAGHRQI 133
              L V G +P  + G Y+RNG    +    +  HLFDG   +  ++FD+G V    R  
Sbjct: 202 VHNLPVTGTVPECIQGVYVRNGANPLH-KPVSGHHLFDGDGMVHAVRFDNGSVSYACRFT 261

Query: 134 ESEAYMAAVRNQKVCFREFSEVPKANNFLAYVGELASLFSGASLTDNSNTGVVKLGD--- 193
           E+   +     ++ C R     PKA      +GEL      A L   +  G+  L D   
Sbjct: 262 ETNRLV----QERECGRPV--FPKA------IGELHGHLGIAKLMLFNTRGLFGLVDPTG 321

Query: 194 -------------GRVVCLTETQ--KGSIMIDSDSLETLGKFEYSDSLGGLIHSAHPIVT 253
                        G ++ ++E        +  +  LET G++++   L   +  AHP + 
Sbjct: 322 GLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTM-IAHPKID 381

Query: 254 DS--EFLTLLPDLFNPGYQVVRMEPGTNERKVIGRVNCRGGPAPGWVHSFPVTENYVVVP 313
               E   L  D+ +  Y          E+     +       P  +H F +TEN+VV+P
Sbjct: 382 PETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEIPL---DQPTMIHDFAITENFVVIP 441

Query: 314 EMPLRYCAQNLLRAEPTPLYKFEWRPESKAFMHVMCKASGKIVTS---VEVPLYITFHFI 373
           +  + +    ++R     +Y      E K     +   + K  +S   +EVP    FH  
Sbjct: 442 DQQVVFRLPEMIRGGSPVVY-----DEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLW 501

Query: 374 NAYEERDEEGRITAVIADCCEHNANPVILDRLRLNNLRRFALKDALPDARVGRFRIPMDG 433
           N++EE + +     V+   C    + +        N     L+  L + R+         
Sbjct: 502 NSWEEPETD---EVVVIGSCMTPPDSIF-------NEHDETLQSVLSEIRLNL------K 561

Query: 434 SGNGTLEAALEPEEHGRGMDMCSFNPAFLGKKYRYAYACGAKRPCNFPNTLTKIDLVEKK 493
           +G  T    +  + +   ++    N   LG+K RYAY      P    +   K+DL   +
Sbjct: 562 TGESTRRPVISEQVN---LEAGMVNRNLLGRKTRYAY-LALTEPWPKVSGFAKVDLSTGE 621

Query: 494 AKNW-HEDGAIPSEPFFVARPGATEEDDGVVISMVSGKNGEGYAL-LLDGSTFEEIARAK 544
            + + + +G    EP F+   G  EED G ++  V  +      L L++    +  A   
Sbjct: 622 IRKYIYGEGKYGGEPLFLP-SGDGEEDGGYIMVFVHDEEKVKSELQLINAVNMKLEATVT 648