MS001169 (gene) Bitter gourd (TR) v1

Overview
NameMS001169
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionSerine hydroxymethyltransferase
Locationscaffold36: 1979353 .. 1983767 (+)
RNA-Seq ExpressionMS001169
SyntenyMS001169
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTACCTATGGCTCTTCGAAGGCTCCTCGTCGCAGTTAGGAAACCTACTGATCCTCTCGTCAATGGCCGGTTCCTCCATCACATGGTTTTTTCTTTTGCCGTCATTGTTATTTCATGATATCTATGTTTTGCATTTGTCATTTTTCGTAACTGTTCCGATGCCGGATTTTGATATTCTTTTTGTATGTGAATTCGTTGTCTGTGGCGGCAGTCTTCTCTGTCCGGGGCGGCTACGGAGGACCGCGAGAAGGCTCGAGCTACTGTGAGCGTTCCATTTTTATGATTCTTGTTTTACATCTGCTTCTGATACTTGTAATGTGAATAAGGACAAAGGCATGTATGTGGATACTGGGGTGTACGAGATCTGTTTTATATGTCGGTTTTCTTGTATTGATATTGATGTTTGGAGGAAATTTTGGTTTCAGTGGATTAAGCAGCTGAACGCCCCCCTCGAGGTCATTGATCCGGAAATTGCAGACATCATTGAGCTCGAGAAAGCTCGTCAATGGAAGGTATATATATATATTTTACTGCGAGCTGTTTTCAGGGTCTTTGTACTTGTGAAAATTTTACGTTGATTTAATCGCTTCTTCATTTTTTAAAGACTTCAAGTAGACGTTGATGGTGTTGATATCCATTGAATTGAGTTGAGTTTTGCGATTAACATCAAAATCATGTACGATTGTTTTGTGAATTTGAAGGGTCTTGAACTCATACCTTCTGAGAATTTCACCTCATCATCAGTGATGGAAGCAGTTGGTTCAGTTATGACCAACAAATACAGTGAAGGATATCCAGGTGCCAGATACTATGGAGGAAATGAGTATGTGATTCTAGATCATTTCCAGAATACAAATTTTTTCTTGCCTTATTGCATAAAGTATTGCATTTTGGATAAGAAGTATCAAAGATGAATTTCTTTCTTAATATTCGGGATGCAATGACTACTTCTCTGATAAGGTTGACGGATTTTGAATTTATAGGATGGAATTTCAGGTTTGCAAAAACAAAATAAGAACATAGTATAGTTTTATGATAGACCATCACGATTTTTTTTTAATGTCAACAAAATCATTGCAAGTTTTAGAACTTGGCAACTTGCAGCGAGGACACTGATTTTTTTTTTTTGGCACATAATTGTTGTTTTTCTTTCCCTCCACTTCTGTCTTTTTTCCTTTTCATTTTCATTCTGGATTCCTAACATAAACGCCAAATGGATTTAGGTACATTGACATGGCAGAATCCTTATGCCAGAAACGTGCATTAGAAGCTTTCTCTTTGGATCCTGCAAAATGGGGAGGTAGGAGAACTATTGATAATTATTAATTTCCAGCGATAAAAATTGGGTCAGACGGCCTATTTACATATTTAGTGGTTTACTGTTTCTTTCCCGCAGTTTCTTGCAAATTCAGCTATATATTGCAAATATTTTCAAAATTTAGTCAGCAAATGTATTTATCATCAACCGGTTGTTCTCAATATGGTGCCATTTATTGGTATAATTAATGACAGAGTGAACGACCCAGATGGAGTTGATTGCAAAACTTTACTAATGTTGTTCTTGTGTTTTGAGGTTTGTTATAGTTTGCACGGGGAAGGGAAAAAACATAGTCTCCCAGATAACCTCCTAACAATTTTCTTCCCATGAAAAGTATTCTATTTCTTTCTAACCAAACTTCGCAAAGCGAAGACCCAACCACATTATGTTACAACAATAGAGGATTTTTTCTTAAAAGGATGACTGTTGCATATATAAATCAGAAAATCAACTGCACCTCCCTTTTTTTTGGACACAGCCTGGGAGGGAAGACATATTGGTGAGCTTTGCTATAGTGCGAACCATAAGAGTGTTGTTGTTTAGGTTGGCTTGTCCTTTATATTTTACCATCTCCAAGACCATTCAATTTGGTCTATGTGAATGCCAGAGCACAATCTTGATTAAACTCAGCATGCTACTTTTTGTAGTTAACGTGCAGTCTCTCTCTGGATCCCCGGCTAACTTTCAGGCTTATACTGCATTATTAAAACCTCATGAGAGGATTATGGCTCTTGATTTACCACATGGTGGACATCTTTCGCATGGTTATCAGGTGTTTAATTTTCAAATTTAGTACTTGGTTTTCACATTGCTGCTTTTGGTGATTTTGTTGGTAATATGAACTTTTTATGATGTTTGACATGATACTTGCCTACGTATGTTTCTTTGAACTTTTGCAGACTGATACGAAGAAGATTTCTGCTGTTTCAATATTCTTTGAGACAATGCCATATAGGTTGGATGAGAGCACTGGTTACATTGACTATGACCAGGTTAGCAAAAATGCTTGTGAGTTGGGACTGTATAGGGATATCCCGTTGATTTTTCTTGAAACTGCAATTCCAAATAAACAAACTTATATGGAGGTAAATTCTAACAGTTGGAGAGAAATGCGGCATTGTTCCGACCAAAACTCATTGTTGCTGGTGCAAGTGCCTATGCCCGTCTCTATGACTATGCACGAATTCGCAAGGTTATTCCTTTGCTTGACTTTAGAGTAGCCCTCTTCAGGTTTTTACACTTCTAATATCATTTAACACATGATTTGACTATGATTATTTTGACAGGTCTGCGACAAACAGAAGGCAATCATGTTGGCCGACATGGCACACATTAGCGGTTTAGTTGCAGCAGGTGTTATTCCATCTCCTTTTGAATATGCTGATATTGTGACAACAACCACACATAAGTCCTTACGTGGACCACGAGGGGCTATGATTTTCTTCCGGAAGGGTGTTAAAGAGATCAACAAAAAAGGACAGGAAGTAAGTGAGGTCTTAAAAATGTAGGAAAGAATACTACTTGCAAGGGAAGGTTCCTACGACACCTAGAAGCAAATATAAGTTTCTAAGGGCAAGTTCTCAGTTGTCTAATTTTTATTACACACAGGTGCTCTATGACTATGAAGACAAAATTAATCAGGCTGTCTTTCCCGGTCTTCAAGGTGGCCCACACAACCACACTATAGCTGGTCTGGCAGTTGCACTAAAGCAGGTTGTTTATTTTTTATTAATTCTCTTTCTCGATAACAAAAGTAAATTTGGGTTTATACTGATGAAATTTTACTTCAGATGCTAGATTTAGCTACTTGAAGTTTCTCCTTGTCTTCATTCATGTTATTTCTGTTATTTTACCATATTGCAATAGGCCACTACTCCGGAATACAAAGCCTACCAAGAGCAGGTTCTCAGCAATTGTTCAAGATTTGCTCAGGTATACAGAAGATGTTTCTCTGCAACTTCTATGACAAATCATCAGTATAATACTCTTCAATTACATATTTTTCTCACTATGTAGAGTAGTTAGTTCTTGAACTTTTTTCTTTGCGTAAGTCATTGTAGTTTACACTGTTAAACCAATTGTTTATGCAGAGTTTGATTGAGAAGGGCTATGAACTTGTATCTGGTGGAACTGACAACCATTTAGTCCTTGTGAATTTAAAAAATAAGGTAAAGGGTGACACTTCTGTTGACCATTATAACTATTATACTTCTCATCCAGTTGGAGAGTTGTTTCTCCTGTCATCAGGTGTTTGTATTTATGTAGTGCTGGGTGCTGACGAATACAAATGTAAAGACAGTTTAACTAACCATAAAAATCTTCCCAACTTATTTTCGAACTTATGATAATATAGGTTCCCTAAAATGAACTTTCATCTATGTTTCTGTTATAACAAGGGGAGTGTAAAGGTTATCCTTTTTGATTGATTTGGACCAATATTTAGAAATGACCGGTAGCATTGGTGAACAACCCTTTTCTTAATATTTTATTTGTTATCATTCGCAGGGTATTGATGGCTCGAGAGTTGAGAAGGTTTTGGAATCAGTTCATATTGCGGCTAACAAGAACACAGTTCCAGGGGACGTGTCTGCCATGGTTCCTGGTGGCATCCGTATGGGTATGACCTTCCAACATTTTGCCTTTGATCTTCAAACTTACTGCTCAAATATCTAAACTTGTAGAAGATTTGTTCTCTGGACTTCTCGTATATATAGGGACGCCTGCTCTGACTTCGAGAGGGTTCCTTGAGGCAGATTTTGCGAAAGTAGCCGAATTCTTTGATGAAGCTGTGAAGTTAGCTTTGAAGATTAAGGCTGATAGCCAAGGTTCTCGAGCATAATATTTATCTGCATTGAAATATCCAGATCTCTCTCTCGTATTGCCCATTTATCGGACTACTGCCTTTTAGAATCCTTTGATATACTCATCTGTGTTTAAATATTGTAAATGCAGGTACTAAGTTGAAGGATTTCGTGGCAACCATGCAATCAAATAAAGACTTTCAATCTGAAATTTCAAAGCTCCGACATCAGGTTGAAGAGTATGCCAAGCAATTTCCAACAATTGGTTTTGAGAAAGAGACAATGCGATACAGAAAC

mRNA sequence

CTACCTATGGCTCTTCGAAGGCTCCTCGTCGCAGTTAGGAAACCTACTGATCCTCTCGTCAATGGCCGGTTCCTCCATCACATGTCTTCTCTGTCCGGGGCGGCTACGGAGGACCGCGAGAAGGCTCGAGCTACTTGGATTAAGCAGCTGAACGCCCCCCTCGAGGTCATTGATCCGGAAATTGCAGACATCATTGAGCTCGAGAAAGCTCGTCAATGGAAGGGTCTTGAACTCATACCTTCTGAGAATTTCACCTCATCATCAGTGATGGAAGCAGTTGGTTCAGTTATGACCAACAAATACAGTGAAGGATATCCAGGTGCCAGATACTATGGAGGAAATGAGTACATTGACATGGCAGAATCCTTATGCCAGAAACGTGCATTAGAAGCTTTCTCTTTGGATCCTGCAAAATGGGGAGTTAACGTGCAGTCTCTCTCTGGATCCCCGGCTAACTTTCAGGCTTATACTGCATTATTAAAACCTCATGAGAGGATTATGGCTCTTGATTTACCACATGGTGGACATCTTTCGCATGGTTATCAGACTGATACGAAGAAGATTTCTGCTGTTTCAATATTCTTTGAGACAATGCCATATAGGTTGGATGAGAGCACTGGTTACATTGACTATGACCAGTTGGAGAGAAATGCGGCATTGTTCCGACCAAAACTCATTGTTGCTGGTGCAAGTGCCTATGCCCGTCTCTATGACTATGCACGAATTCGCAAGGTCTGCGACAAACAGAAGGCAATCATGTTGGCCGACATGGCACACATTAGCGGTTTAGTTGCAGCAGGTGTTATTCCATCTCCTTTTGAATATGCTGATATTGTGACAACAACCACACATAAGTCCTTACGTGGACCACGAGGGGCTATGATTTTCTTCCGGAAGGGTGTTAAAGAGATCAACAAAAAAGGACAGGAAGTGCTCTATGACTATGAAGACAAAATTAATCAGGCTGTCTTTCCCGGTCTTCAAGGTGGCCCACACAACCACACTATAGCTGGTCTGGCAGTTGCACTAAAGCAGGCCACTACTCCGGAATACAAAGCCTACCAAGAGCAGGTTCTCAGCAATTGTTCAAGATTTGCTCAGAGTTTGATTGAGAAGGGCTATGAACTTGTATCTGGTGGAACTGACAACCATTTAGTCCTTGTGAATTTAAAAAATAAGGGTATTGATGGCTCGAGAGTTGAGAAGGTTTTGGAATCAGTTCATATTGCGGCTAACAAGAACACAGTTCCAGGGGACGTGTCTGCCATGGTTCCTGGTGGCATCCGTATGGGGACGCCTGCTCTGACTTCGAGAGGGTTCCTTGAGGCAGATTTTGCGAAAGTAGCCGAATTCTTTGATGAAGCTGTGAAGTTAGCTTTGAAGATTAAGGCTGATAGCCAAGGTACTAAGTTGAAGGATTTCGTGGCAACCATGCAATCAAATAAAGACTTTCAATCTGAAATTTCAAAGCTCCGACATCAGGTTGAAGAGTATGCCAAGCAATTTCCAACAATTGGTTTTGAGAAAGAGACAATGCGATACAGAAAC

Coding sequence (CDS)

CTACCTATGGCTCTTCGAAGGCTCCTCGTCGCAGTTAGGAAACCTACTGATCCTCTCGTCAATGGCCGGTTCCTCCATCACATGTCTTCTCTGTCCGGGGCGGCTACGGAGGACCGCGAGAAGGCTCGAGCTACTTGGATTAAGCAGCTGAACGCCCCCCTCGAGGTCATTGATCCGGAAATTGCAGACATCATTGAGCTCGAGAAAGCTCGTCAATGGAAGGGTCTTGAACTCATACCTTCTGAGAATTTCACCTCATCATCAGTGATGGAAGCAGTTGGTTCAGTTATGACCAACAAATACAGTGAAGGATATCCAGGTGCCAGATACTATGGAGGAAATGAGTACATTGACATGGCAGAATCCTTATGCCAGAAACGTGCATTAGAAGCTTTCTCTTTGGATCCTGCAAAATGGGGAGTTAACGTGCAGTCTCTCTCTGGATCCCCGGCTAACTTTCAGGCTTATACTGCATTATTAAAACCTCATGAGAGGATTATGGCTCTTGATTTACCACATGGTGGACATCTTTCGCATGGTTATCAGACTGATACGAAGAAGATTTCTGCTGTTTCAATATTCTTTGAGACAATGCCATATAGGTTGGATGAGAGCACTGGTTACATTGACTATGACCAGTTGGAGAGAAATGCGGCATTGTTCCGACCAAAACTCATTGTTGCTGGTGCAAGTGCCTATGCCCGTCTCTATGACTATGCACGAATTCGCAAGGTCTGCGACAAACAGAAGGCAATCATGTTGGCCGACATGGCACACATTAGCGGTTTAGTTGCAGCAGGTGTTATTCCATCTCCTTTTGAATATGCTGATATTGTGACAACAACCACACATAAGTCCTTACGTGGACCACGAGGGGCTATGATTTTCTTCCGGAAGGGTGTTAAAGAGATCAACAAAAAAGGACAGGAAGTGCTCTATGACTATGAAGACAAAATTAATCAGGCTGTCTTTCCCGGTCTTCAAGGTGGCCCACACAACCACACTATAGCTGGTCTGGCAGTTGCACTAAAGCAGGCCACTACTCCGGAATACAAAGCCTACCAAGAGCAGGTTCTCAGCAATTGTTCAAGATTTGCTCAGAGTTTGATTGAGAAGGGCTATGAACTTGTATCTGGTGGAACTGACAACCATTTAGTCCTTGTGAATTTAAAAAATAAGGGTATTGATGGCTCGAGAGTTGAGAAGGTTTTGGAATCAGTTCATATTGCGGCTAACAAGAACACAGTTCCAGGGGACGTGTCTGCCATGGTTCCTGGTGGCATCCGTATGGGGACGCCTGCTCTGACTTCGAGAGGGTTCCTTGAGGCAGATTTTGCGAAAGTAGCCGAATTCTTTGATGAAGCTGTGAAGTTAGCTTTGAAGATTAAGGCTGATAGCCAAGGTACTAAGTTGAAGGATTTCGTGGCAACCATGCAATCAAATAAAGACTTTCAATCTGAAATTTCAAAGCTCCGACATCAGGTTGAAGAGTATGCCAAGCAATTTCCAACAATTGGTTTTGAGAAAGAGACAATGCGATACAGAAAC

Protein sequence

LPMALRRLLVAVRKPTDPLVNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDPEIADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDYARIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKGVKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLSNCSRFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQGTKLKDFVATMQSNKDFQSEISKLRHQVEEYAKQFPTIGFEKETMRYRN
Homology
BLAST of MS001169 vs. NCBI nr
Match: XP_022131877.1 (serine hydroxymethyltransferase 2, mitochondrial [Momordica charantia])

HSP 1 Score: 1018.5 bits (2632), Expect = 2.1e-293
Identity = 513/514 (99.81%), Postives = 513/514 (99.81%), Query Frame = 0

Query: 3   MALRRLLVAVRKPTDPLVNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDPEIA 62
           MALRRLLVAVRKPTDPLVNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDPEIA
Sbjct: 1   MALRRLLVAVRKPTDPLVNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDPEIA 60

Query: 63  DIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMAES 122
           DIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMAES
Sbjct: 61  DIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMAES 120

Query: 123 LCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHGYQ 182
           LCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHGYQ
Sbjct: 121 LCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHGYQ 180

Query: 183 TDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDYARI 242
           TDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDYARI
Sbjct: 181 TDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDYARI 240

Query: 243 RKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKGVK 302
           RKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKGVK
Sbjct: 241 RKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKGVK 300

Query: 303 EINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLSNC 362
           EINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLSNC
Sbjct: 301 EINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLSNC 360

Query: 363 SRFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDVSA 422
           SRFAQSLIEKGYELVS GTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDVSA
Sbjct: 361 SRFAQSLIEKGYELVSAGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDVSA 420

Query: 423 MVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQGTKLKDFVATMQSNK 482
           MVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQGTKLKDFVATMQSNK
Sbjct: 421 MVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQGTKLKDFVATMQSNK 480

Query: 483 DFQSEISKLRHQVEEYAKQFPTIGFEKETMRYRN 517
           DFQSEISKLRHQVEEYAKQFPTIGFEKETMRYRN
Sbjct: 481 DFQSEISKLRHQVEEYAKQFPTIGFEKETMRYRN 514

BLAST of MS001169 vs. NCBI nr
Match: XP_038885497.1 (serine hydroxymethyltransferase 2, mitochondrial [Benincasa hispida])

HSP 1 Score: 976.1 bits (2522), Expect = 1.2e-280
Identity = 490/516 (94.96%), Postives = 500/516 (96.90%), Query Frame = 0

Query: 1   LPMALRRLLVAVRKPTDPLVNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDPE 60
           + MALRR+   VRKP  PLVN RFLHHMSSLSGAA E REKARATWIKQLNAPLEVIDPE
Sbjct: 1   MAMALRRIAFGVRKPILPLVNARFLHHMSSLSGAAAEQREKARATWIKQLNAPLEVIDPE 60

Query: 61  IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYID+A
Sbjct: 61  IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDIA 120

Query: 121 ESLCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           ESLCQKRALEAF LDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG
Sbjct: 121 ESLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180

Query: 181 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLER+A+LFRPKLIVAGASAYARLYDYA
Sbjct: 181 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSASLFRPKLIVAGASAYARLYDYA 240

Query: 241 RIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIRKVCDKQKA+MLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG
Sbjct: 241 RIRKVCDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS 360
           VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS
Sbjct: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS 360

Query: 361 NCSRFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           NCS+FAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV
Sbjct: 361 NCSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQGTKLKDFVATMQS 480
           SAMVPGGIRMGTPALTSRGFLEADF KVAEFFDE VKLAL+IKA S+GTKLKDFVATMQS
Sbjct: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEFFDETVKLALQIKAGSEGTKLKDFVATMQS 480

Query: 481 NKDFQSEISKLRHQVEEYAKQFPTIGFEKETMRYRN 517
           NKD QSEIS LR QVEEYAKQFPTIGFEKETMRYR+
Sbjct: 481 NKDIQSEISNLRQQVEEYAKQFPTIGFEKETMRYRD 516

BLAST of MS001169 vs. NCBI nr
Match: XP_004148411.1 (serine hydroxymethyltransferase 2, mitochondrial [Cucumis sativus] >KAE8652150.1 hypothetical protein Csa_022369 [Cucumis sativus])

HSP 1 Score: 975.3 bits (2520), Expect = 2.0e-280
Identity = 489/516 (94.77%), Postives = 500/516 (96.90%), Query Frame = 0

Query: 1   LPMALRRLLVAVRKPTDPLVNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDPE 60
           + MALRR+ V V  P  PL+N RFLH MSSLSGAA E+REKARATWI+QLNAPLEVIDPE
Sbjct: 1   MAMALRRIAVGVTNPILPLLNARFLHRMSSLSGAAAEEREKARATWIQQLNAPLEVIDPE 60

Query: 61  IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIELEKARQWKG ELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 61  IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120

Query: 121 ESLCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           ESLCQKRALEAF LDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG
Sbjct: 121 ESLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180

Query: 181 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLER+A+LFRPKLIVAGASAYARLYDYA
Sbjct: 181 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSASLFRPKLIVAGASAYARLYDYA 240

Query: 241 RIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIRKVCDKQKA+MLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG
Sbjct: 241 RIRKVCDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS 360
           VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS
Sbjct: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS 360

Query: 361 NCSRFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           NCS+FAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV
Sbjct: 361 NCSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQGTKLKDFVATMQS 480
           SAMVPGGIRMGTPALTSRGFLEADF KVAE FDE VKLALKIKA S+GTKLKDFVATMQS
Sbjct: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAELFDETVKLALKIKAGSEGTKLKDFVATMQS 480

Query: 481 NKDFQSEISKLRHQVEEYAKQFPTIGFEKETMRYRN 517
           NKDFQSEISKLRHQVEEYAKQFPTIGFEKETMRYR+
Sbjct: 481 NKDFQSEISKLRHQVEEYAKQFPTIGFEKETMRYRD 516

BLAST of MS001169 vs. NCBI nr
Match: XP_008444959.1 (PREDICTED: serine hydroxymethyltransferase 2, mitochondrial [Cucumis melo])

HSP 1 Score: 973.4 bits (2515), Expect = 7.7e-280
Identity = 488/516 (94.57%), Postives = 500/516 (96.90%), Query Frame = 0

Query: 1   LPMALRRLLVAVRKPTDPLVNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDPE 60
           + MALRR+ V V  P  PL+N RFLH MSSLS AA E+REKARATWI+QLNAPLEVIDPE
Sbjct: 1   MAMALRRIAVGVTNPMLPLLNARFLHRMSSLSEAAAEEREKARATWIQQLNAPLEVIDPE 60

Query: 61  IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 61  IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120

Query: 121 ESLCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           ESLCQKRALEAF LDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG
Sbjct: 121 ESLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180

Query: 181 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLER+A+LFRPKLIVAGASAYARLYDYA
Sbjct: 181 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSASLFRPKLIVAGASAYARLYDYA 240

Query: 241 RIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIRKVCDKQKA+MLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG
Sbjct: 241 RIRKVCDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS 360
           VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS
Sbjct: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS 360

Query: 361 NCSRFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           NCS+FAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV
Sbjct: 361 NCSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQGTKLKDFVATMQS 480
           SAMVPGGIRMGTPALTSRGFLEADF KVAEFFDE VKLALKIK  S+GTKLKDFVATMQS
Sbjct: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEFFDETVKLALKIKGGSEGTKLKDFVATMQS 480

Query: 481 NKDFQSEISKLRHQVEEYAKQFPTIGFEKETMRYRN 517
           NKDFQ+EISKLRHQVEEYAKQFPTIGFEKETMRYR+
Sbjct: 481 NKDFQAEISKLRHQVEEYAKQFPTIGFEKETMRYRD 516

BLAST of MS001169 vs. NCBI nr
Match: KAA0065075.1 (serine hydroxymethyltransferase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 968.8 bits (2503), Expect = 1.9e-278
Identity = 488/517 (94.39%), Postives = 500/517 (96.71%), Query Frame = 0

Query: 1   LPMALRRLLVAVRKPTDPLVNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDPE 60
           + MALRR+ V V  P  PL+N RFLH MSSLS AA E+REKARATWI+QLNAPLEVIDPE
Sbjct: 1   MAMALRRIAVGVTNPMLPLLNARFLHRMSSLSEAAAEEREKARATWIQQLNAPLEVIDPE 60

Query: 61  IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 61  IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120

Query: 121 ESLCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           ESLCQKRALEAF LDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG
Sbjct: 121 ESLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180

Query: 181 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLER+A+LFRPKLIVAGASAYARLYDYA
Sbjct: 181 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSASLFRPKLIVAGASAYARLYDYA 240

Query: 241 RIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIRKVCDKQKA+MLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG
Sbjct: 241 RIRKVCDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS 360
           VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS
Sbjct: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS 360

Query: 361 NCSRFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           NCS+FAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV
Sbjct: 361 NCSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQG-TKLKDFVATMQ 480
           SAMVPGGIRMGTPALTSRGFLEADF KVAEFFDE VKLALKIK  S+G TKLKDFVATMQ
Sbjct: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEFFDETVKLALKIKGGSEGCTKLKDFVATMQ 480

Query: 481 SNKDFQSEISKLRHQVEEYAKQFPTIGFEKETMRYRN 517
           SNKDFQ+EISKLRHQVEEYAKQFPTIGFEKETMRYR+
Sbjct: 481 SNKDFQAEISKLRHQVEEYAKQFPTIGFEKETMRYRD 517

BLAST of MS001169 vs. ExPASy Swiss-Prot
Match: Q94C74 (Serine hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SHM2 PE=1 SV=2)

HSP 1 Score: 895.2 bits (2312), Expect = 3.5e-259
Identity = 445/516 (86.24%), Postives = 482/516 (93.41%), Query Frame = 0

Query: 1   LPMALRRLLVAVRKPTDPL-VNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDP 60
           + +ALRRL  +V+KP   L  NG  L  MSSLS AA  + EK+R++WIKQLNA L+ IDP
Sbjct: 1   MALALRRLSSSVKKPISLLSSNGGSLRFMSSLSTAAMAESEKSRSSWIKQLNASLDEIDP 60

Query: 61  EIADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDM 120
           E+ADIIELEKARQWKG ELIPSENFTS SVM+AVGSVMTNKYSEGYPGARYYGGNEYIDM
Sbjct: 61  EVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 120

Query: 121 AESLCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSH 180
           AE+LCQKRALEAF LDP+KWGVNVQSLSGSPANFQ YTALLKPHERIMALDLPHGGHLSH
Sbjct: 121 AETLCQKRALEAFQLDPSKWGVNVQSLSGSPANFQVYTALLKPHERIMALDLPHGGHLSH 180

Query: 181 GYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDY 240
           GYQTDTKKISAVSIFFETMPYRLDE+TGYIDYDQLE++A LFRPKLIVAGASAYARLYDY
Sbjct: 181 GYQTDTKKISAVSIFFETMPYRLDENTGYIDYDQLEKSAVLFRPKLIVAGASAYARLYDY 240

Query: 241 ARIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRK 300
           ARIRKVC+KQKA+MLADMAHISGLVAAGVIPSPFEYAD+VTTTTHKSLRGPRGAMIFFRK
Sbjct: 241 ARIRKVCNKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRK 300

Query: 301 GVKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVL 360
           G+KEINK+G+EV+YDYED+INQAVFPGLQGGPHNHTI GLAVALKQA TPEYKAYQ+QVL
Sbjct: 301 GLKEINKQGKEVMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYKAYQDQVL 360

Query: 361 SNCSRFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGD 420
            NCS+FA++L+ KGY+LVSGGTDNHLVLVNLKNKGIDGSRVEKVLE VHIAANKNTVPGD
Sbjct: 361 RNCSKFAETLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGD 420

Query: 421 VSAMVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQGTKLKDFVATMQ 480
           VSAMVPGGIRMGTPALTSRGF+E DFAKVAE+FD AVK+ALKIKA+SQGTKLKDFVATMQ
Sbjct: 421 VSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAVKIALKIKAESQGTKLKDFVATMQ 480

Query: 481 SNKDFQSEISKLRHQVEEYAKQFPTIGFEKETMRYR 516
           SN+  QSE+SKLR  VEEYAKQFPTIGFEKETMRY+
Sbjct: 481 SNEKLQSEMSKLREMVEEYAKQFPTIGFEKETMRYK 516

BLAST of MS001169 vs. ExPASy Swiss-Prot
Match: P50433 (Serine hydroxymethyltransferase, mitochondrial OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 894.0 bits (2309), Expect = 7.8e-259
Identity = 441/516 (85.47%), Postives = 478/516 (92.64%), Query Frame = 0

Query: 1   LPMALRRLLVAVRKPTDPLVNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDPE 60
           + +ALRRL   V KP   L NG  L++MSSL   A  D+EK+   W KQLNAPLEV+DPE
Sbjct: 3   MAIALRRLSATVDKPVKSLYNGGSLYYMSSLPNEAVYDKEKSGVAWPKQLNAPLEVVDPE 62

Query: 61  IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIE EKARQWKGLELIPSENFTS SVM+AVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 63  IADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMA 122

Query: 121 ESLCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           E+LCQKRALEAF LDPAKWGVNVQ LSGSPANFQ YTALLKPHERIMALDLPHGGHLSHG
Sbjct: 123 ETLCQKRALEAFRLDPAKWGVNVQPLSGSPANFQVYTALLKPHERIMALDLPHGGHLSHG 182

Query: 181 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLE++A LFRPKLIVAGASAYARLYDY 
Sbjct: 183 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYDYD 242

Query: 241 RIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIRKVC+KQKAI+LADMAHISGLVAAGVIPSPF+YAD+VTTTTHKSLRGPRGAMIF+RKG
Sbjct: 243 RIRKVCNKQKAILLADMAHISGLVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFYRKG 302

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS 360
           VKE+NK+G+EV YDYEDKINQAVFPGLQGGPHNHTI GLAVALKQATTPEY+AYQEQVLS
Sbjct: 303 VKEVNKQGKEVFYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYRAYQEQVLS 362

Query: 361 NCSRFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           N S+FAQ+L EKGYELVSGGTDNHLVLVN+KNKGIDGSRVEKVLE+VHIAANKNTVPGDV
Sbjct: 363 NSSKFAQALGEKGYELVSGGTDNHLVLVNMKNKGIDGSRVEKVLEAVHIAANKNTVPGDV 422

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQGTKLKDFVATMQS 480
           SAMVPGGIRMGTPALTSRGFLE DF KVA+FFD AVK+A+K+KA++QGTKLKDFVAT++S
Sbjct: 423 SAMVPGGIRMGTPALTSRGFLEEDFVKVADFFDAAVKIAVKVKAETQGTKLKDFVATLES 482

Query: 481 NKDFQSEISKLRHQVEEYAKQFPTIGFEKETMRYRN 517
           +   +SEI+KLRH VEEYAKQFPTIGFEKETM+Y+N
Sbjct: 483 SAPIKSEIAKLRHDVEEYAKQFPTIGFEKETMKYKN 518

BLAST of MS001169 vs. ExPASy Swiss-Prot
Match: Q9SZJ5 (Serine hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SHM1 PE=1 SV=1)

HSP 1 Score: 878.2 bits (2268), Expect = 4.4e-254
Identity = 432/516 (83.72%), Postives = 478/516 (92.64%), Query Frame = 0

Query: 1   LPMALRRLLVAVRKPTDPLVNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDPE 60
           + MALRRL  ++ KP  PL+      +MSSL   A +++E++R TW KQLNAPLE +DPE
Sbjct: 3   MAMALRRLSSSIDKPIRPLIRSTSC-YMSSLPSEAVDEKERSRVTWPKQLNAPLEEVDPE 62

Query: 61  IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIE EKARQWKGLELIPSENFTS SVM+AVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 63  IADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMA 122

Query: 121 ESLCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           E+LCQKRALEAF LDP KWGVNVQ LSGSPANF  YTALLKPHERIMALDLPHGGHLSHG
Sbjct: 123 ETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVYTALLKPHERIMALDLPHGGHLSHG 182

Query: 181 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQ+E++A LFRPKLIVAGASAYARLYDYA
Sbjct: 183 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQMEKSATLFRPKLIVAGASAYARLYDYA 242

Query: 241 RIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIRKVC+KQKA+MLADMAHISGLVAA VIPSPF+YAD+VTTTTHKSLRGPRGAMIFFRKG
Sbjct: 243 RIRKVCNKQKAVMLADMAHISGLVAANVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKG 302

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS 360
           VKEINK+G+EVLYD+EDKINQAVFPGLQGGPHNHTI GLAVALKQATT EYKAYQEQVLS
Sbjct: 303 VKEINKQGKEVLYDFEDKINQAVFPGLQGGPHNHTITGLAVALKQATTSEYKAYQEQVLS 362

Query: 361 NCSRFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           N ++FAQ+L+E+GYELVSGGTDNHLVLVNLK KGIDGSRVEKVLE+VHIA+NKNTVPGDV
Sbjct: 363 NSAKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEKVLEAVHIASNKNTVPGDV 422

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQGTKLKDFVATMQS 480
           SAMVPGGIRMGTPALTSRGF+E DFAKVAE+FD+AV +ALK+K+++QGTKLKDFV+ M+S
Sbjct: 423 SAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKVKSEAQGTKLKDFVSAMES 482

Query: 481 NKDFQSEISKLRHQVEEYAKQFPTIGFEKETMRYRN 517
           +   QSEI+KLRH+VEE+AKQFPTIGFEKETM+Y+N
Sbjct: 483 SSTIQSEIAKLRHEVEEFAKQFPTIGFEKETMKYKN 517

BLAST of MS001169 vs. ExPASy Swiss-Prot
Match: P34899 (Serine hydroxymethyltransferase, mitochondrial OS=Pisum sativum OX=3888 PE=1 SV=1)

HSP 1 Score: 878.2 bits (2268), Expect = 4.4e-254
Identity = 433/514 (84.24%), Postives = 473/514 (92.02%), Query Frame = 0

Query: 1   LPMALRRLLVAVRKPTDPLVNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDPE 60
           + MALR+L  +V K + PL +   L++ SSL   A  D+E  R TW KQLN+PLEVIDPE
Sbjct: 3   MAMALRKLSSSVNKSSRPLFSASSLYYKSSLPDEAVYDKENPRVTWPKQLNSPLEVIDPE 62

Query: 61  IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIELEKARQWKGLELIPSENFTS SVM+AVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 63  IADIIELEKARQWKGLELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMA 122

Query: 121 ESLCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           E+LCQKRALEAF LDPAKWGVNVQ LSGSP+NFQ YTALLKPH+RIMALDLPHGGHLSHG
Sbjct: 123 ETLCQKRALEAFRLDPAKWGVNVQPLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHG 182

Query: 181 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLE++A LFRPKLIVAGASAYARLYDYA
Sbjct: 183 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYDYA 242

Query: 241 RIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIRKVCDKQKA++LADMAHISGLVAAGVIPSPF+YAD+VTTTTHKSLRGPRGAMIFFRKG
Sbjct: 243 RIRKVCDKQKAVLLADMAHISGLVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKG 302

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS 360
           +KE+NK+G+EV YDYEDKINQAVFPGLQGGPHNHTI GLAVALKQATTPEY+AYQEQVLS
Sbjct: 303 LKEVNKQGKEVFYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYRAYQEQVLS 362

Query: 361 NCSRFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           N S+FA++L EKGY+LVSGGT+NHLVLVNLKNKGIDGSRVEKVLE VHIAANKNTVPGDV
Sbjct: 363 NSSKFAKALSEKGYDLVSGGTENHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGDV 422

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQGTKLKDFVATMQS 480
           SAMVPGGIRMGTPALTSRGF+E DF KVAE+FD AV LALK+KA+S+GTKLKDFV  +Q+
Sbjct: 423 SAMVPGGIRMGTPALTSRGFVEEDFVKVAEYFDAAVSLALKVKAESKGTKLKDFVEALQT 482

Query: 481 NKDFQSEISKLRHQVEEYAKQFPTIGFEKETMRY 515
           +   QSEISKL+H VEE+AKQFPTIGFEK TM+Y
Sbjct: 483 SSYVQSEISKLKHDVEEFAKQFPTIGFEKATMKY 516

BLAST of MS001169 vs. ExPASy Swiss-Prot
Match: P49358 (Serine hydroxymethyltransferase 2, mitochondrial OS=Flaveria pringlei OX=4226 PE=2 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 3.7e-253
Identity = 438/513 (85.38%), Postives = 471/513 (91.81%), Query Frame = 0

Query: 4   ALRRLLVAVRKPTDPLVNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDPEIAD 63
           ALRRL  +  KP   L NG  L+ MSSL   A  ++E+   TW KQLNAPLEV DPEIAD
Sbjct: 6   ALRRLSSSSNKPLQRLFNGGHLYSMSSLPSEAVYEKERPGVTWPKQLNAPLEVGDPEIAD 65

Query: 64  IIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMAESL 123
           IIELEKARQWKGLELI SENFTS SVM+AVGSVMTNKYSEGYPGARYYGGNEYIDMAE+L
Sbjct: 66  IIELEKARQWKGLELILSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAETL 125

Query: 124 CQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHGYQT 183
           CQKRALEAF LD AKWGVNVQ LSGSPANF  YTALLK H+RIMALDLPHGGHLSHGYQT
Sbjct: 126 CQKRALEAFRLDAAKWGVNVQPLSGSPANFHVYTALLKAHDRIMALDLPHGGHLSHGYQT 185

Query: 184 DTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDYARIR 243
           DTKKISAVSIFFETMPYRL+ESTGYIDYDQLE++A LFRPKLIVAGASAYARLYDYARIR
Sbjct: 186 DTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYDYARIR 245

Query: 244 KVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKGVKE 303
           KVCDKQKAI+LADMAHISGLVAAGVIPSPF+YAD+VTTTTHKSLRGPRGAMIFFRKGVKE
Sbjct: 246 KVCDKQKAILLADMAHISGLVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKGVKE 305

Query: 304 INKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLSNCS 363
           +NK+G+EVLYDYEDKINQAVFPGLQGGPHNHTI GLAVALKQATT EYKAYQEQV+SNC+
Sbjct: 306 VNKQGKEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTAEYKAYQEQVMSNCA 365

Query: 364 RFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDVSAM 423
           +FA++L++ GYELVSGGT+NHLVLVNLKNKGIDGSRVEKVLE+VHIAANKNTVPGDVSAM
Sbjct: 366 KFAETLVKSGYELVSGGTENHLVLVNLKNKGIDGSRVEKVLEAVHIAANKNTVPGDVSAM 425

Query: 424 VPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQGTKLKDFVATMQSNKD 483
           VPGGIRMGTPALTSRGF+E DFAKVA  FD AVKLA+KIK ++QGTKLKDFVA MQS+  
Sbjct: 426 VPGGIRMGTPALTSRGFVEEDFAKVAYLFDLAVKLAVKIKGEAQGTKLKDFVAAMQSSA- 485

Query: 484 FQSEISKLRHQVEEYAKQFPTIGFEKETMRYRN 517
           FQSEISKLRH VEEYAKQFPTIGFEKETM+Y+N
Sbjct: 486 FQSEISKLRHDVEEYAKQFPTIGFEKETMKYKN 517

BLAST of MS001169 vs. ExPASy TrEMBL
Match: A0A6J1BS86 (Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111004911 PE=3 SV=1)

HSP 1 Score: 1018.5 bits (2632), Expect = 1.0e-293
Identity = 513/514 (99.81%), Postives = 513/514 (99.81%), Query Frame = 0

Query: 3   MALRRLLVAVRKPTDPLVNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDPEIA 62
           MALRRLLVAVRKPTDPLVNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDPEIA
Sbjct: 1   MALRRLLVAVRKPTDPLVNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDPEIA 60

Query: 63  DIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMAES 122
           DIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMAES
Sbjct: 61  DIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMAES 120

Query: 123 LCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHGYQ 182
           LCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHGYQ
Sbjct: 121 LCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHGYQ 180

Query: 183 TDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDYARI 242
           TDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDYARI
Sbjct: 181 TDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDYARI 240

Query: 243 RKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKGVK 302
           RKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKGVK
Sbjct: 241 RKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKGVK 300

Query: 303 EINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLSNC 362
           EINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLSNC
Sbjct: 301 EINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLSNC 360

Query: 363 SRFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDVSA 422
           SRFAQSLIEKGYELVS GTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDVSA
Sbjct: 361 SRFAQSLIEKGYELVSAGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDVSA 420

Query: 423 MVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQGTKLKDFVATMQSNK 482
           MVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQGTKLKDFVATMQSNK
Sbjct: 421 MVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQGTKLKDFVATMQSNK 480

Query: 483 DFQSEISKLRHQVEEYAKQFPTIGFEKETMRYRN 517
           DFQSEISKLRHQVEEYAKQFPTIGFEKETMRYRN
Sbjct: 481 DFQSEISKLRHQVEEYAKQFPTIGFEKETMRYRN 514

BLAST of MS001169 vs. ExPASy TrEMBL
Match: A0A1S3BBK2 (Serine hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103488144 PE=3 SV=1)

HSP 1 Score: 973.4 bits (2515), Expect = 3.7e-280
Identity = 488/516 (94.57%), Postives = 500/516 (96.90%), Query Frame = 0

Query: 1   LPMALRRLLVAVRKPTDPLVNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDPE 60
           + MALRR+ V V  P  PL+N RFLH MSSLS AA E+REKARATWI+QLNAPLEVIDPE
Sbjct: 1   MAMALRRIAVGVTNPMLPLLNARFLHRMSSLSEAAAEEREKARATWIQQLNAPLEVIDPE 60

Query: 61  IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 61  IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120

Query: 121 ESLCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           ESLCQKRALEAF LDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG
Sbjct: 121 ESLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180

Query: 181 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLER+A+LFRPKLIVAGASAYARLYDYA
Sbjct: 181 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSASLFRPKLIVAGASAYARLYDYA 240

Query: 241 RIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIRKVCDKQKA+MLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG
Sbjct: 241 RIRKVCDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS 360
           VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS
Sbjct: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS 360

Query: 361 NCSRFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           NCS+FAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV
Sbjct: 361 NCSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQGTKLKDFVATMQS 480
           SAMVPGGIRMGTPALTSRGFLEADF KVAEFFDE VKLALKIK  S+GTKLKDFVATMQS
Sbjct: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEFFDETVKLALKIKGGSEGTKLKDFVATMQS 480

Query: 481 NKDFQSEISKLRHQVEEYAKQFPTIGFEKETMRYRN 517
           NKDFQ+EISKLRHQVEEYAKQFPTIGFEKETMRYR+
Sbjct: 481 NKDFQAEISKLRHQVEEYAKQFPTIGFEKETMRYRD 516

BLAST of MS001169 vs. ExPASy TrEMBL
Match: A0A5A7VGP9 (Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004050 PE=3 SV=1)

HSP 1 Score: 968.8 bits (2503), Expect = 9.2e-279
Identity = 488/517 (94.39%), Postives = 500/517 (96.71%), Query Frame = 0

Query: 1   LPMALRRLLVAVRKPTDPLVNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDPE 60
           + MALRR+ V V  P  PL+N RFLH MSSLS AA E+REKARATWI+QLNAPLEVIDPE
Sbjct: 1   MAMALRRIAVGVTNPMLPLLNARFLHRMSSLSEAAAEEREKARATWIQQLNAPLEVIDPE 60

Query: 61  IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 61  IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120

Query: 121 ESLCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           ESLCQKRALEAF LDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG
Sbjct: 121 ESLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180

Query: 181 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLER+A+LFRPKLIVAGASAYARLYDYA
Sbjct: 181 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSASLFRPKLIVAGASAYARLYDYA 240

Query: 241 RIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIRKVCDKQKA+MLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG
Sbjct: 241 RIRKVCDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS 360
           VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS
Sbjct: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS 360

Query: 361 NCSRFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           NCS+FAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV
Sbjct: 361 NCSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQG-TKLKDFVATMQ 480
           SAMVPGGIRMGTPALTSRGFLEADF KVAEFFDE VKLALKIK  S+G TKLKDFVATMQ
Sbjct: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEFFDETVKLALKIKGGSEGCTKLKDFVATMQ 480

Query: 481 SNKDFQSEISKLRHQVEEYAKQFPTIGFEKETMRYRN 517
           SNKDFQ+EISKLRHQVEEYAKQFPTIGFEKETMRYR+
Sbjct: 481 SNKDFQAEISKLRHQVEEYAKQFPTIGFEKETMRYRD 517

BLAST of MS001169 vs. ExPASy TrEMBL
Match: A0A6J1KI05 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111495957 PE=3 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.3e-277
Identity = 482/516 (93.41%), Postives = 501/516 (97.09%), Query Frame = 0

Query: 1   LPMALRRLLVAVRKPTDPLVNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDPE 60
           + MALRRL V V K   PLVNGRFLHHMSSLSGAA E+R+KARATWIKQLNAPL VIDPE
Sbjct: 1   MAMALRRLAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDPE 60

Query: 61  IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIELEKARQWKG ELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 61  IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120

Query: 121 ESLCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           E+LCQKRALEAF LDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG
Sbjct: 121 ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180

Query: 181 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFET+PYRLDESTGYIDYDQLE++A+LFRPKLIVAGASAYARLYDYA
Sbjct: 181 YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA 240

Query: 241 RIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIR++CDKQKA+MLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG
Sbjct: 241 RIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS 360
           VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTI GLAVALKQATTPEYKAYQEQVLS
Sbjct: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS 360

Query: 361 NCSRFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           N S+FAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV
Sbjct: 361 NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQGTKLKDFVATMQS 480
           SAMVPGGIRMGTPALTSRGFLEADF KVAE+FDEAVKLALKIKA+S+GTKLKDFVATMQS
Sbjct: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS 480

Query: 481 NKDFQSEISKLRHQVEEYAKQFPTIGFEKETMRYRN 517
           NKDFQSEISKLR +VE+YAKQFPTIGFEKETM+YR+
Sbjct: 481 NKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 516

BLAST of MS001169 vs. ExPASy TrEMBL
Match: A0A6J1GHA4 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454162 PE=3 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 3.0e-277
Identity = 480/516 (93.02%), Postives = 501/516 (97.09%), Query Frame = 0

Query: 1   LPMALRRLLVAVRKPTDPLVNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDPE 60
           + MALRR  V V K   PLVNGRFLHHMSSLSGAA E+R+KARATWIKQLNAPLEVIDPE
Sbjct: 1   MAMALRRFAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLEVIDPE 60

Query: 61  IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIELEKARQWKG ELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 61  IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120

Query: 121 ESLCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           E+LCQKRALEAF LDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG
Sbjct: 121 ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180

Query: 181 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFET+PYRLDESTGYIDYDQLE++A+LFRPKLIVAGASAYARLYDYA
Sbjct: 181 YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA 240

Query: 241 RIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIR++CDKQKA+MLADMAHISGLVAAGVIPSPF+YAD+VTTTTHKSLRGPRGAMIFFRKG
Sbjct: 241 RIRQICDKQKAVMLADMAHISGLVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKG 300

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS 360
           VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTI GLAVALKQATTPEYKAYQEQVLS
Sbjct: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS 360

Query: 361 NCSRFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           N S+FAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV
Sbjct: 361 NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQGTKLKDFVATMQS 480
           SAMVPGGIRMGTPALTSRGFLEADF KVAE+FDEAVKLALKIKA+S+GTKLKDFVATMQS
Sbjct: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS 480

Query: 481 NKDFQSEISKLRHQVEEYAKQFPTIGFEKETMRYRN 517
           NKDFQSEISKLR +VE+YAKQFPTIGFEKETM+YR+
Sbjct: 481 NKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 516

BLAST of MS001169 vs. TAIR 10
Match: AT5G26780.1 (serine hydroxymethyltransferase 2 )

HSP 1 Score: 895.2 bits (2312), Expect = 2.5e-260
Identity = 445/516 (86.24%), Postives = 482/516 (93.41%), Query Frame = 0

Query: 1   LPMALRRLLVAVRKPTDPL-VNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDP 60
           + +ALRRL  +V+KP   L  NG  L  MSSLS AA  + EK+R++WIKQLNA L+ IDP
Sbjct: 1   MALALRRLSSSVKKPISLLSSNGGSLRFMSSLSTAAMAESEKSRSSWIKQLNASLDEIDP 60

Query: 61  EIADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDM 120
           E+ADIIELEKARQWKG ELIPSENFTS SVM+AVGSVMTNKYSEGYPGARYYGGNEYIDM
Sbjct: 61  EVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 120

Query: 121 AESLCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSH 180
           AE+LCQKRALEAF LDP+KWGVNVQSLSGSPANFQ YTALLKPHERIMALDLPHGGHLSH
Sbjct: 121 AETLCQKRALEAFQLDPSKWGVNVQSLSGSPANFQVYTALLKPHERIMALDLPHGGHLSH 180

Query: 181 GYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDY 240
           GYQTDTKKISAVSIFFETMPYRLDE+TGYIDYDQLE++A LFRPKLIVAGASAYARLYDY
Sbjct: 181 GYQTDTKKISAVSIFFETMPYRLDENTGYIDYDQLEKSAVLFRPKLIVAGASAYARLYDY 240

Query: 241 ARIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRK 300
           ARIRKVC+KQKA+MLADMAHISGLVAAGVIPSPFEYAD+VTTTTHKSLRGPRGAMIFFRK
Sbjct: 241 ARIRKVCNKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRK 300

Query: 301 GVKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVL 360
           G+KEINK+G+EV+YDYED+INQAVFPGLQGGPHNHTI GLAVALKQA TPEYKAYQ+QVL
Sbjct: 301 GLKEINKQGKEVMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYKAYQDQVL 360

Query: 361 SNCSRFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGD 420
            NCS+FA++L+ KGY+LVSGGTDNHLVLVNLKNKGIDGSRVEKVLE VHIAANKNTVPGD
Sbjct: 361 RNCSKFAETLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGD 420

Query: 421 VSAMVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQGTKLKDFVATMQ 480
           VSAMVPGGIRMGTPALTSRGF+E DFAKVAE+FD AVK+ALKIKA+SQGTKLKDFVATMQ
Sbjct: 421 VSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAVKIALKIKAESQGTKLKDFVATMQ 480

Query: 481 SNKDFQSEISKLRHQVEEYAKQFPTIGFEKETMRYR 516
           SN+  QSE+SKLR  VEEYAKQFPTIGFEKETMRY+
Sbjct: 481 SNEKLQSEMSKLREMVEEYAKQFPTIGFEKETMRYK 516

BLAST of MS001169 vs. TAIR 10
Match: AT5G26780.3 (serine hydroxymethyltransferase 2 )

HSP 1 Score: 885.9 bits (2288), Expect = 1.5e-257
Identity = 446/532 (83.83%), Postives = 482/532 (90.60%), Query Frame = 0

Query: 1   LPMALRRLLVAVRKPTDPL-VNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDP 60
           + +ALRRL  +V+KP   L  NG  L  MSSLS AA  + EK+R++WIKQLNA L+ IDP
Sbjct: 1   MALALRRLSSSVKKPISLLSSNGGSLRFMSSLSTAAMAESEKSRSSWIKQLNASLDEIDP 60

Query: 61  EIADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDM 120
           E+ADIIELEKARQWKG ELIPSENFTS SVM+AVGSVMTNKYSEGYPGARYYGGNEYIDM
Sbjct: 61  EVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 120

Query: 121 AESLCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSH 180
           AE+LCQKRALEAF LDP+KWGVNVQSLSGSPANFQ YTALLKPHERIMALDLPHGGHLSH
Sbjct: 121 AETLCQKRALEAFQLDPSKWGVNVQSLSGSPANFQVYTALLKPHERIMALDLPHGGHLSH 180

Query: 181 GYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDY 240
           GYQTDTKKISAVSIFFETMPYRLDE+TGYIDYDQLE++A LFRPKLIVAGASAYARLYDY
Sbjct: 181 GYQTDTKKISAVSIFFETMPYRLDENTGYIDYDQLEKSAVLFRPKLIVAGASAYARLYDY 240

Query: 241 ARIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRK 300
           ARIRKVC+KQKA+MLADMAHISGLVAAGVIPSPFEYAD+VTTTTHKSLRGPRGAMIFFRK
Sbjct: 241 ARIRKVCNKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRK 300

Query: 301 GVKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVL 360
           G+KEINK+G+EV+YDYED+INQAVFPGLQGGPHNHTI GLAVALKQA TPEYKAYQ+QVL
Sbjct: 301 GLKEINKQGKEVMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYKAYQDQVL 360

Query: 361 SNCSRFA----------------QSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKV 420
            NCS+FA                Q+L+ KGY+LVSGGTDNHLVLVNLKNKGIDGSRVEKV
Sbjct: 361 RNCSKFAELDIRPTVIISYGLSMQTLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKV 420

Query: 421 LESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIK 480
           LE VHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGF+E DFAKVAE+FD AVK+ALKIK
Sbjct: 421 LELVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAVKIALKIK 480

Query: 481 ADSQGTKLKDFVATMQSNKDFQSEISKLRHQVEEYAKQFPTIGFEKETMRYR 516
           A+SQGTKLKDFVATMQSN+  QSE+SKLR  VEEYAKQFPTIGFEKETMRY+
Sbjct: 481 AESQGTKLKDFVATMQSNEKLQSEMSKLREMVEEYAKQFPTIGFEKETMRYK 532

BLAST of MS001169 vs. TAIR 10
Match: AT5G26780.2 (serine hydroxymethyltransferase 2 )

HSP 1 Score: 885.9 bits (2288), Expect = 1.5e-257
Identity = 446/532 (83.83%), Postives = 482/532 (90.60%), Query Frame = 0

Query: 1   LPMALRRLLVAVRKPTDPL-VNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDP 60
           + +ALRRL  +V+KP   L  NG  L  MSSLS AA  + EK+R++WIKQLNA L+ IDP
Sbjct: 1   MALALRRLSSSVKKPISLLSSNGGSLRFMSSLSTAAMAESEKSRSSWIKQLNASLDEIDP 60

Query: 61  EIADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDM 120
           E+ADIIELEKARQWKG ELIPSENFTS SVM+AVGSVMTNKYSEGYPGARYYGGNEYIDM
Sbjct: 61  EVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 120

Query: 121 AESLCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSH 180
           AE+LCQKRALEAF LDP+KWGVNVQSLSGSPANFQ YTALLKPHERIMALDLPHGGHLSH
Sbjct: 121 AETLCQKRALEAFQLDPSKWGVNVQSLSGSPANFQVYTALLKPHERIMALDLPHGGHLSH 180

Query: 181 GYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDY 240
           GYQTDTKKISAVSIFFETMPYRLDE+TGYIDYDQLE++A LFRPKLIVAGASAYARLYDY
Sbjct: 181 GYQTDTKKISAVSIFFETMPYRLDENTGYIDYDQLEKSAVLFRPKLIVAGASAYARLYDY 240

Query: 241 ARIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRK 300
           ARIRKVC+KQKA+MLADMAHISGLVAAGVIPSPFEYAD+VTTTTHKSLRGPRGAMIFFRK
Sbjct: 241 ARIRKVCNKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRK 300

Query: 301 GVKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVL 360
           G+KEINK+G+EV+YDYED+INQAVFPGLQGGPHNHTI GLAVALKQA TPEYKAYQ+QVL
Sbjct: 301 GLKEINKQGKEVMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYKAYQDQVL 360

Query: 361 SNCSRFA----------------QSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKV 420
            NCS+FA                Q+L+ KGY+LVSGGTDNHLVLVNLKNKGIDGSRVEKV
Sbjct: 361 RNCSKFAELDIRPTVIISYGLSMQTLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKV 420

Query: 421 LESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIK 480
           LE VHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGF+E DFAKVAE+FD AVK+ALKIK
Sbjct: 421 LELVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAVKIALKIK 480

Query: 481 ADSQGTKLKDFVATMQSNKDFQSEISKLRHQVEEYAKQFPTIGFEKETMRYR 516
           A+SQGTKLKDFVATMQSN+  QSE+SKLR  VEEYAKQFPTIGFEKETMRY+
Sbjct: 481 AESQGTKLKDFVATMQSNEKLQSEMSKLREMVEEYAKQFPTIGFEKETMRYK 532

BLAST of MS001169 vs. TAIR 10
Match: AT4G37930.1 (serine transhydroxymethyltransferase 1 )

HSP 1 Score: 878.2 bits (2268), Expect = 3.1e-255
Identity = 432/516 (83.72%), Postives = 478/516 (92.64%), Query Frame = 0

Query: 1   LPMALRRLLVAVRKPTDPLVNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDPE 60
           + MALRRL  ++ KP  PL+      +MSSL   A +++E++R TW KQLNAPLE +DPE
Sbjct: 3   MAMALRRLSSSIDKPIRPLIRSTSC-YMSSLPSEAVDEKERSRVTWPKQLNAPLEEVDPE 62

Query: 61  IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIE EKARQWKGLELIPSENFTS SVM+AVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 63  IADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMA 122

Query: 121 ESLCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           E+LCQKRALEAF LDP KWGVNVQ LSGSPANF  YTALLKPHERIMALDLPHGGHLSHG
Sbjct: 123 ETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVYTALLKPHERIMALDLPHGGHLSHG 182

Query: 181 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQ+E++A LFRPKLIVAGASAYARLYDYA
Sbjct: 183 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQMEKSATLFRPKLIVAGASAYARLYDYA 242

Query: 241 RIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIRKVC+KQKA+MLADMAHISGLVAA VIPSPF+YAD+VTTTTHKSLRGPRGAMIFFRKG
Sbjct: 243 RIRKVCNKQKAVMLADMAHISGLVAANVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKG 302

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS 360
           VKEINK+G+EVLYD+EDKINQAVFPGLQGGPHNHTI GLAVALKQATT EYKAYQEQVLS
Sbjct: 303 VKEINKQGKEVLYDFEDKINQAVFPGLQGGPHNHTITGLAVALKQATTSEYKAYQEQVLS 362

Query: 361 NCSRFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           N ++FAQ+L+E+GYELVSGGTDNHLVLVNLK KGIDGSRVEKVLE+VHIA+NKNTVPGDV
Sbjct: 363 NSAKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEKVLEAVHIASNKNTVPGDV 422

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQGTKLKDFVATMQS 480
           SAMVPGGIRMGTPALTSRGF+E DFAKVAE+FD+AV +ALK+K+++QGTKLKDFV+ M+S
Sbjct: 423 SAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKVKSEAQGTKLKDFVSAMES 482

Query: 481 NKDFQSEISKLRHQVEEYAKQFPTIGFEKETMRYRN 517
           +   QSEI+KLRH+VEE+AKQFPTIGFEKETM+Y+N
Sbjct: 483 SSTIQSEIAKLRHEVEEFAKQFPTIGFEKETMKYKN 517

BLAST of MS001169 vs. TAIR 10
Match: AT4G32520.1 (serine hydroxymethyltransferase 3 )

HSP 1 Score: 565.8 bits (1457), Expect = 3.4e-161
Identity = 278/451 (61.64%), Postives = 341/451 (75.61%), Query Frame = 0

Query: 57  IDPEIADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEY 116
           +DPE+  II  EK RQ++ LELI SENFTS +VMEAVGS +TNKYSEG PG RYYGGNEY
Sbjct: 86  VDPEVRTIITKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEY 145

Query: 117 IDMAESLCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGH 176
           ID  E+LCQ RAL AF LD  KWGVNVQ LSGSPANF  YTA+L PH+RIM LDLPHGGH
Sbjct: 146 IDQLETLCQNRALAAFRLDSTKWGVNVQPLSGSPANFAVYTAILSPHDRIMGLDLPHGGH 205

Query: 177 LSHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARL 236
           LSHG+ T  +++S  SI+FE+MPYRLDESTG +DYD LE+ A LFRPKLI+AGASAY+R 
Sbjct: 206 LSHGFMTAKRRVSGTSIYFESMPYRLDESTGIVDYDMLEKTATLFRPKLIIAGASAYSRD 265

Query: 237 YDYARIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIF 296
           +DY R+RK+ D   A ++ DMAHISGLVAA V+  PFEY DIVTTTTHKSLRGPRG MIF
Sbjct: 266 FDYPRMRKIADSVGAFLMMDMAHISGLVAASVVADPFEYCDIVTTTTHKSLRGPRGGMIF 325

Query: 297 FRKGVKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQE 356
           FRK    IN        D E  +N AVFPGLQGGPHNHTI GLAV LK A +PE+KAYQ+
Sbjct: 326 FRK--DPING------VDLESAVNNAVFPGLQGGPHNHTIGGLAVCLKHAQSPEFKAYQK 385

Query: 357 QVLSNCSRFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTV 416
           +V+SNC   A  L+E G++LVSGG+DNHLVLV+L+  G+DG+RVEK+L+   I  NKN+V
Sbjct: 386 RVVSNCRALANRLVELGFKLVSGGSDNHLVLVDLRPMGMDGARVEKILDMASITLNKNSV 445

Query: 417 PGDVSAMVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQGTKLKDFVA 476
           PGD SA+VPGGIR+G+PA+T+RG  E DF  VA+F  E V++ ++ K  + G+KL+DF  
Sbjct: 446 PGDKSALVPGGIRIGSPAMTTRGLSEKDFVVVADFIKEGVEITMEAKKAAPGSKLQDFNK 505

Query: 477 TMQSNK-DFQSEISKLRHQVEEYAKQFPTIG 507
            + S +   +  +  L+ +VE +  +FP  G
Sbjct: 506 FVTSPEFPLKERVKSLKERVETFTSRFPIPG 528

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022131877.12.1e-29399.81serine hydroxymethyltransferase 2, mitochondrial [Momordica charantia][more]
XP_038885497.11.2e-28094.96serine hydroxymethyltransferase 2, mitochondrial [Benincasa hispida][more]
XP_004148411.12.0e-28094.77serine hydroxymethyltransferase 2, mitochondrial [Cucumis sativus] >KAE8652150.1... [more]
XP_008444959.17.7e-28094.57PREDICTED: serine hydroxymethyltransferase 2, mitochondrial [Cucumis melo][more]
KAA0065075.11.9e-27894.39serine hydroxymethyltransferase 2 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q94C743.5e-25986.24Serine hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis thaliana OX=3702... [more]
P504337.8e-25985.47Serine hydroxymethyltransferase, mitochondrial OS=Solanum tuberosum OX=4113 PE=2... [more]
Q9SZJ54.4e-25483.72Serine hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702... [more]
P348994.4e-25484.24Serine hydroxymethyltransferase, mitochondrial OS=Pisum sativum OX=3888 PE=1 SV=... [more]
P493583.7e-25385.38Serine hydroxymethyltransferase 2, mitochondrial OS=Flaveria pringlei OX=4226 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1BS861.0e-29399.81Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111004911 P... [more]
A0A1S3BBK23.7e-28094.57Serine hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103488144 PE=3 SV=... [more]
A0A5A7VGP99.2e-27994.39Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A6J1KI052.3e-27793.41Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111495957 PE=3... [more]
A0A6J1GHA43.0e-27793.02Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454162 PE... [more]
Match NameE-valueIdentityDescription
AT5G26780.12.5e-26086.24serine hydroxymethyltransferase 2 [more]
AT5G26780.31.5e-25783.83serine hydroxymethyltransferase 2 [more]
AT5G26780.21.5e-25783.83serine hydroxymethyltransferase 2 [more]
AT4G37930.13.1e-25583.72serine transhydroxymethyltransferase 1 [more]
AT4G32520.13.4e-16161.64serine hydroxymethyltransferase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 481..501
NoneNo IPR availablePIRSRPIRSR000412-50PIRSR000412-50coord: 52..506
e-value: 5.6E-162
score: 537.0
NoneNo IPR availablePANTHERPTHR11680:SF49SERINE HYDROXYMETHYLTRANSFERASEcoord: 4..515
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 83..356
e-value: 1.3E-217
score: 724.4
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 58..503
e-value: 1.3E-217
score: 724.4
IPR039429Serine hydroxymethyltransferase-like domainPFAMPF00464SHMTcoord: 55..451
e-value: 1.6E-213
score: 708.6
IPR001085Serine hydroxymethyltransferasePIRSFPIRSF000412SHMTcoord: 35..511
e-value: 9.2E-171
score: 565.9
IPR001085Serine hydroxymethyltransferasePANTHERPTHR11680SERINE HYDROXYMETHYLTRANSFERASEcoord: 4..515
IPR001085Serine hydroxymethyltransferaseHAMAPMF_00051SHMTcoord: 53..506
score: 42.853245
IPR001085Serine hydroxymethyltransferaseCDDcd00378SHMTcoord: 58..478
e-value: 0.0
score: 637.708
IPR019798Serine hydroxymethyltransferase, pyridoxal phosphate binding sitePROSITEPS00096SHMTcoord: 277..293
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 50..506

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS001169.1MS001169.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019264 glycine biosynthetic process from serine
biological_process GO:0035999 tetrahydrofolate interconversion
molecular_function GO:0003824 catalytic activity
molecular_function GO:0004372 glycine hydroxymethyltransferase activity
molecular_function GO:0030170 pyridoxal phosphate binding