MS001160 (gene) Bitter gourd (TR) v1

Overview
NameMS001160
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionAdenine nucleotide alpha hydrolases-like superfamily protein
Locationscaffold36: 1908128 .. 1909575 (+)
RNA-Seq ExpressionMS001160
SyntenyMS001160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGAGAATCTCCGGTGCGTGATCGTGGCCGTCGACGGCAGCGAGGAGAGCATGGGCGCTTTACGGTGGGCGCTTGAAAATCTCAAGCTCCGGTCAGCGGCGCCTGATTCCACTGACGGCACTTTTGTAGCGCTACATGTTCAATCTCCTCCTTCAATCGCCGCCGGTCTCAGTCCTGGTCCAATCCCCTTTGGCGGCCCTAGTTGAGTCGAGTCCTGTACTAATTTTCTCACGCTCATTTCTGAGCTGTTATCGAGAGTTCTCTGAATTTAATGTTTTAATTGGATGTAGGCGATCTGGAGGTTCCTGCTTTCGCGGCAGCAATCGAGTCGCACCAGAGGCGGATTACAGCTGCGATTTTCGACCATGCTTCACGGATTTGCTCCGAGTACAAGGTTAGCTCTATTGTTCAGAAGCTCTCGCAGCAATTCGTTTTCCTTGTCAGTGTGTGGTGGAGTATGTTATACCATAAATTAAACAGTAAAGAAGAGCAATCTCAGTGAACTTCAAGTCATTTTTTAGTTTTTGTCATTTCTTTTATTCTCTTCTCCTGAAACAGACGAAATGATTTTGGTAATGAATATCTAATAACTAGCTCCAACAATGTGTATATGTGGTGTATTCTTGCTCGTGTTTATAGTTTTCTCAATATTTCCGGGGCATCAATTTCAATTCCACGTATCGTTTTATCCTGTTTGAACTGAAGGTTGAAGCGAATTTCATTATGTGATTTTCAGGTGAATGTGGAAACTAAAGTAGTGATCGGAGATCCAAAGGAAAAGATCTGTGAAGTGGCAGAGAATTTGCATGCTGATTTGCTAGTTATGGGATCCCGTGCTTTTGGTCCCATCAAAAGGTATGGTTATATGATAGATTTCCGTAATTAGTCTTTGTAATGGTCAATCTATACTTTTCTGCAAGCCACACTGTAATCAGCTAGTAGTTGTTGCTTGTTGGTGTGAATCTTTACAGCATTGTTTGATTTTGTGATGCATGTGGCAACCAATGAGAAAATAGGCTCAGTTACTTCAGTCTGTACTGTATTACTTTCAAGTGTTCTGTTGACGTAGTTACAGATTCTAATTGCATTGTTTTACTTTGAGAAAAAACATCTCGCTGCATCCAAGAATTTGTCGAATCATTTGCTTGCATATCCCAAGTATTCCAGTCTGGCCTCCGAATTAAGAGTTAAGCTAACAATATCAATGGCCATATGTTTGTTTCATGTTTTATTATAGTTTGTTATTGTATGCTAGATCTCCATCATTAATGTAATTCCACATATTTTTTTGGAAAATAACATCTGAAGTGAAGGGTTATTGACACGCCTTTGTGATCATACCGCTTTGTACAGGATGTTCTTGGGAAGTGTAAGCAACTATTGTACCAACCATGTTGAATGCCCAGTCATCATAGTCAAGGACAAGAAAAGGGGCAGTGCGTCA

mRNA sequence

ATGTCTGAGAATCTCCGGTGCGTGATCGTGGCCGTCGACGGCAGCGAGGAGAGCATGGGCGCTTTACGGTGGGCGCTTGAAAATCTCAAGCTCCGGTCAGCGGCGCCTGATTCCACTGACGGCACTTTTGTAGCGCTACATGTTCAATCTCCTCCTTCAATCGCCGCCGGTCTCAGTCCTGGTCCAATCCCCTTTGGCGGCCCTAGCGATCTGGAGGTTCCTGCTTTCGCGGCAGCAATCGAGTCGCACCAGAGGCGGATTACAGCTGCGATTTTCGACCATGCTTCACGGATTTGCTCCGAGTACAAGGTGAATGTGGAAACTAAAGTAGTGATCGGAGATCCAAAGGAAAAGATCTGTGAAGTGGCAGAGAATTTGCATGCTGATTTGCTAGTTATGGGATCCCGTGCTTTTGGTCCCATCAAAAGGATGTTCTTGGGAAGTGTAAGCAACTATTGTACCAACCATGTTGAATGCCCAGTCATCATAGTCAAGGACAAGAAAAGGGGCAGTGCGTCA

Coding sequence (CDS)

ATGTCTGAGAATCTCCGGTGCGTGATCGTGGCCGTCGACGGCAGCGAGGAGAGCATGGGCGCTTTACGGTGGGCGCTTGAAAATCTCAAGCTCCGGTCAGCGGCGCCTGATTCCACTGACGGCACTTTTGTAGCGCTACATGTTCAATCTCCTCCTTCAATCGCCGCCGGTCTCAGTCCTGGTCCAATCCCCTTTGGCGGCCCTAGCGATCTGGAGGTTCCTGCTTTCGCGGCAGCAATCGAGTCGCACCAGAGGCGGATTACAGCTGCGATTTTCGACCATGCTTCACGGATTTGCTCCGAGTACAAGGTGAATGTGGAAACTAAAGTAGTGATCGGAGATCCAAAGGAAAAGATCTGTGAAGTGGCAGAGAATTTGCATGCTGATTTGCTAGTTATGGGATCCCGTGCTTTTGGTCCCATCAAAAGGATGTTCTTGGGAAGTGTAAGCAACTATTGTACCAACCATGTTGAATGCCCAGTCATCATAGTCAAGGACAAGAAAAGGGGCAGTGCGTCA

Protein sequence

MSENLRCVIVAVDGSEESMGALRWALENLKLRSAAPDSTDGTFVALHVQSPPSIAAGLSPGPIPFGGPSDLEVPAFAAAIESHQRRITAAIFDHASRICSEYKVNVETKVVIGDPKEKICEVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSAS
Homology
BLAST of MS001160 vs. NCBI nr
Match: XP_022132206.1 (universal stress protein PHOS34 [Momordica charantia] >XP_022132207.1 universal stress protein PHOS34 [Momordica charantia])

HSP 1 Score: 347.8 bits (891), Expect = 5.3e-92
Identity = 173/173 (100.00%), Postives = 173/173 (100.00%), Query Frame = 0

Query: 1   MSENLRCVIVAVDGSEESMGALRWALENLKLRSAAPDSTDGTFVALHVQSPPSIAAGLSP 60
           MSENLRCVIVAVDGSEESMGALRWALENLKLRSAAPDSTDGTFVALHVQSPPSIAAGLSP
Sbjct: 1   MSENLRCVIVAVDGSEESMGALRWALENLKLRSAAPDSTDGTFVALHVQSPPSIAAGLSP 60

Query: 61  GPIPFGGPSDLEVPAFAAAIESHQRRITAAIFDHASRICSEYKVNVETKVVIGDPKEKIC 120
           GPIPFGGPSDLEVPAFAAAIESHQRRITAAIFDHASRICSEYKVNVETKVVIGDPKEKIC
Sbjct: 61  GPIPFGGPSDLEVPAFAAAIESHQRRITAAIFDHASRICSEYKVNVETKVVIGDPKEKIC 120

Query: 121 EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSAS 174
           EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSAS
Sbjct: 121 EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSAS 173

BLAST of MS001160 vs. NCBI nr
Match: KAG6598738.1 (Universal stress protein A-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 329.7 bits (844), Expect = 1.5e-86
Identity = 161/173 (93.06%), Postives = 167/173 (96.53%), Query Frame = 0

Query: 1   MSENLRCVIVAVDGSEESMGALRWALENLKLRSAAPDSTDGTFVALHVQSPPSIAAGLSP 60
           MS +LRCVIVAVDGSEESMGALRWA ENLKLRS+APDSTDGTF+ LHVQ PP+IAAGLSP
Sbjct: 1   MSADLRCVIVAVDGSEESMGALRWAFENLKLRSSAPDSTDGTFIVLHVQPPPTIAAGLSP 60

Query: 61  GPIPFGGPSDLEVPAFAAAIESHQRRITAAIFDHASRICSEYKVNVETKVVIGDPKEKIC 120
           GPIPFGGPSDLEVPAF AAIESHQRRITAAI DHASRICSEYKVNVETKVVIGDPKEKIC
Sbjct: 61  GPIPFGGPSDLEVPAFTAAIESHQRRITAAILDHASRICSEYKVNVETKVVIGDPKEKIC 120

Query: 121 EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSAS 174
           EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGS++
Sbjct: 121 EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSSA 173

BLAST of MS001160 vs. NCBI nr
Match: XP_023547229.1 (universal stress protein PHOS34 [Cucurbita pepo subsp. pepo] >XP_023547230.1 universal stress protein PHOS34 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 328.2 bits (840), Expect = 4.4e-86
Identity = 161/173 (93.06%), Postives = 165/173 (95.38%), Query Frame = 0

Query: 1   MSENLRCVIVAVDGSEESMGALRWALENLKLRSAAPDSTDGTFVALHVQSPPSIAAGLSP 60
           MS +LRCVIVAVDGSEESMGALRWA ENLKLRS+ PDSTDGTFV LHVQ PP+IAAGLSP
Sbjct: 1   MSADLRCVIVAVDGSEESMGALRWAFENLKLRSSTPDSTDGTFVVLHVQPPPTIAAGLSP 60

Query: 61  GPIPFGGPSDLEVPAFAAAIESHQRRITAAIFDHASRICSEYKVNVETKVVIGDPKEKIC 120
           GPIPFGGPSDLEVPAF AAIESHQRRITAAI DHASRICSEYKVNVETKVVIGDPKEKIC
Sbjct: 61  GPIPFGGPSDLEVPAFTAAIESHQRRITAAILDHASRICSEYKVNVETKVVIGDPKEKIC 120

Query: 121 EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSAS 174
           E AENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGS+S
Sbjct: 121 EAAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSSS 173

BLAST of MS001160 vs. NCBI nr
Match: XP_022961627.1 (universal stress protein PHOS34-like [Cucurbita moschata] >XP_022961628.1 universal stress protein PHOS34-like [Cucurbita moschata])

HSP 1 Score: 326.2 bits (835), Expect = 1.7e-85
Identity = 159/173 (91.91%), Postives = 165/173 (95.38%), Query Frame = 0

Query: 1   MSENLRCVIVAVDGSEESMGALRWALENLKLRSAAPDSTDGTFVALHVQSPPSIAAGLSP 60
           MS +LRCVIVAVDGSEESMGALRWA ENLKLRS+APDS DGTF+ LHVQ PP+IAAGLSP
Sbjct: 1   MSADLRCVIVAVDGSEESMGALRWAFENLKLRSSAPDSNDGTFIVLHVQPPPTIAAGLSP 60

Query: 61  GPIPFGGPSDLEVPAFAAAIESHQRRITAAIFDHASRICSEYKVNVETKVVIGDPKEKIC 120
           GPIPFGGPSDLEVPAF AAIESHQRRITAAI DHASRICSEYKVNVETKVVIGDPKEKIC
Sbjct: 61  GPIPFGGPSDLEVPAFTAAIESHQRRITAAILDHASRICSEYKVNVETKVVIGDPKEKIC 120

Query: 121 EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSAS 174
           E AENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGS++
Sbjct: 121 EAAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSSA 173

BLAST of MS001160 vs. NCBI nr
Match: XP_022997274.1 (universal stress protein PHOS34 [Cucurbita maxima] >XP_022997275.1 universal stress protein PHOS34 [Cucurbita maxima])

HSP 1 Score: 323.6 bits (828), Expect = 1.1e-84
Identity = 159/173 (91.91%), Postives = 165/173 (95.38%), Query Frame = 0

Query: 1   MSENLRCVIVAVDGSEESMGALRWALENLKLRSAAPDSTDGTFVALHVQSPPSIAAGLSP 60
           MS +LRCVIVAVDGSEESMGALRWA ENLKLRS+AP STDGTFV LHVQ PP+IAAGLSP
Sbjct: 1   MSADLRCVIVAVDGSEESMGALRWAFENLKLRSSAPGSTDGTFVVLHVQPPPTIAAGLSP 60

Query: 61  GPIPFGGPSDLEVPAFAAAIESHQRRITAAIFDHASRICSEYKVNVETKVVIGDPKEKIC 120
           GPIPFGGPSDLEVPAF AAIESHQRRITAAI  HASRICS+YKVNVETKVVIGDPKEKIC
Sbjct: 61  GPIPFGGPSDLEVPAFTAAIESHQRRITAAILGHASRICSDYKVNVETKVVIGDPKEKIC 120

Query: 121 EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSAS 174
           EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGS++
Sbjct: 121 EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSSA 173

BLAST of MS001160 vs. ExPASy Swiss-Prot
Match: Q8L4N1 (Universal stress protein PHOS34 OS=Arabidopsis thaliana OX=3702 GN=PHOS34 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.0e-13
Identity = 59/182 (32.42%), Postives = 91/182 (50.00%), Query Frame = 0

Query: 6   RCVIVAVDGSEESMGALRWALEN-------LKLRSAAPDST--DGTFVALHVQSPPSIAA 65
           R + VAVD SEES  A+RWA+++       + +   +P S      +  L +Q+PP  +A
Sbjct: 45  RKIGVAVDLSEESAFAVRWAVDHYIRPGDAVVILHVSPTSVLFGADWGPLPLQTPPPPSA 104

Query: 66  GLSPGPIPFGGPSDLEVPAFAAA-IESHQRRITAAIFDHASRICSEYKVNVETKVVIGDP 125
              PG  P   PS  +  AF ++ +    + +  A F H   I  ++           D 
Sbjct: 105 ATDPGAQP--KPSQEDFDAFTSSKVADLAKPLKEAGFPHKIHIVKDH-----------DM 164

Query: 126 KEKICEVAENLHADLLVMGSRAFGPIKRMF---LGSVSNYCTNHVECPVIIVK--DKKRG 173
           +E++C   E L+   ++MGSR FG  KR     LGSVS+YC +H  CPV++V+  D + G
Sbjct: 165 RERLCLETERLNLSAVIMGSRGFGAEKRGSDGKLGSVSDYCVHHCVCPVVVVRYPDDRDG 213

BLAST of MS001160 vs. ExPASy Swiss-Prot
Match: Q8LGG8 (Universal stress protein A-like protein OS=Arabidopsis thaliana OX=3702 GN=At3g01520 PE=1 SV=2)

HSP 1 Score: 69.7 bits (169), Expect = 3.7e-11
Identity = 28/67 (41.79%), Postives = 42/67 (62.69%), Query Frame = 0

Query: 99  CSEYKVNVETKVVIGDPKEKICEVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVE 158
           C E  V  E  +  GDPK+ IC+  + +  D LV+GSR  G  +++F+G+VS +C  H E
Sbjct: 96  CHEIGVGCEAWIKTGDPKDVICQEVKRVRPDFLVVGSRGLGRFQKVFVGTVSAFCVKHAE 155

Query: 159 CPVIIVK 166
           CPV+ +K
Sbjct: 156 CPVMTIK 162

BLAST of MS001160 vs. ExPASy Swiss-Prot
Match: Q8VYN9 (Universal stress protein PHOS32 OS=Arabidopsis thaliana OX=3702 GN=PHOS32 PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 8.2e-11
Identity = 57/174 (32.76%), Postives = 82/174 (47.13%), Query Frame = 0

Query: 6   RCVIVAVDGSEESMGALRWALENLKLRSAAPDSTDGTFVALHVQSPPSIAAGLSPGPIPF 65
           R + VAVD SEES  A+RWA+++      A        V LHV SP S+  G   GP+P 
Sbjct: 48  RKIGVAVDLSEESSFAVRWAVDHYIRPGDA-------VVLLHV-SPTSVLFGADWGPLPL 107

Query: 66  ----------GGPSDLEVPAFAAAIESHQRRITAAIFDHASRICSEYKVNVETKVVIGDP 125
                       PS  +  AF +          A +      +   YK+++   V   D 
Sbjct: 108 KTQIEDPNAQPQPSQEDFDAFTST-------KVADLAKPLKELGFPYKIHI---VKDHDM 167

Query: 126 KEKICEVAENLHADLLVMGSRAFGPIKRM----FLGSVSNYCTNHVECPVIIVK 166
           +E++C   E L    ++MGSR FG  K+      LGSVS+YC +H  CPV++V+
Sbjct: 168 RERLCLEIERLGLSAVIMGSRGFGAEKKRGSDGKLGSVSDYCVHHCVCPVVVVR 203

BLAST of MS001160 vs. ExPASy Swiss-Prot
Match: O07552 (Stress response protein NhaX OS=Bacillus subtilis (strain 168) OX=224308 GN=nhaX PE=2 SV=2)

HSP 1 Score: 60.1 bits (144), Expect = 2.9e-08
Identity = 50/164 (30.49%), Postives = 78/164 (47.56%), Query Frame = 0

Query: 8   VIVAVDGSEESMGALRWALENLKLRSAA------PDSTDGTFVALHVQSPPSIAAGLSPG 67
           +IVA DGSE S  AL  A++  K  +AA       D  D   V +    P + A+ +S G
Sbjct: 7   IIVAFDGSENSKKALLTAIDLAKTVNAAITVAHSHDMKDNQTV-IDPPRPAAEASYISGG 66

Query: 68  PIPFGGPSDLEVPAFAAAIESHQRRITAAIFDHASRICSEYKVNVETKVVIGDPKEKICE 127
                 P   +V +    I   +   T  +   A  + +E + + +  ++ GDP E I E
Sbjct: 67  MTSVPDPLISDVTSPEPMIYEDR---TEEVIAEARMMLNEQQADGDIDILEGDPAESIIE 126

Query: 128 VAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVK 166
            A  + AD++V GSR    +K++  GSVS   +   + PV+IVK
Sbjct: 127 HANRISADMIVTGSRDQNRLKKLIFGSVSEKLSAKSDIPVLIVK 166

BLAST of MS001160 vs. ExPASy Swiss-Prot
Match: Q57951 (Universal stress protein MJ0531 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0531 PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.5e-07
Identity = 26/73 (35.62%), Postives = 44/73 (60.27%), Query Frame = 0

Query: 97  RICSEYKVNVETKVVIGDPKEKICEVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNH 156
           ++  E+ V + T+++ G P  +I E AE   ADL+VMG+     ++R+ LGSV+     +
Sbjct: 97  KMAEEWGVKIHTEMLEGVPANEIVEFAEKKKADLIVMGTTGKTGLERILLGSVAERVIKN 156

Query: 157 VECPVIIVKDKKR 170
             CPV++VK  K+
Sbjct: 157 AHCPVLVVKKPKK 169

BLAST of MS001160 vs. ExPASy TrEMBL
Match: A0A6J1BRL9 (universal stress protein PHOS34 OS=Momordica charantia OX=3673 GN=LOC111005122 PE=4 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 2.6e-92
Identity = 173/173 (100.00%), Postives = 173/173 (100.00%), Query Frame = 0

Query: 1   MSENLRCVIVAVDGSEESMGALRWALENLKLRSAAPDSTDGTFVALHVQSPPSIAAGLSP 60
           MSENLRCVIVAVDGSEESMGALRWALENLKLRSAAPDSTDGTFVALHVQSPPSIAAGLSP
Sbjct: 1   MSENLRCVIVAVDGSEESMGALRWALENLKLRSAAPDSTDGTFVALHVQSPPSIAAGLSP 60

Query: 61  GPIPFGGPSDLEVPAFAAAIESHQRRITAAIFDHASRICSEYKVNVETKVVIGDPKEKIC 120
           GPIPFGGPSDLEVPAFAAAIESHQRRITAAIFDHASRICSEYKVNVETKVVIGDPKEKIC
Sbjct: 61  GPIPFGGPSDLEVPAFAAAIESHQRRITAAIFDHASRICSEYKVNVETKVVIGDPKEKIC 120

Query: 121 EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSAS 174
           EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSAS
Sbjct: 121 EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSAS 173

BLAST of MS001160 vs. ExPASy TrEMBL
Match: A0A6J1HCC8 (universal stress protein PHOS34-like OS=Cucurbita moschata OX=3662 GN=LOC111462345 PE=4 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 8.0e-86
Identity = 159/173 (91.91%), Postives = 165/173 (95.38%), Query Frame = 0

Query: 1   MSENLRCVIVAVDGSEESMGALRWALENLKLRSAAPDSTDGTFVALHVQSPPSIAAGLSP 60
           MS +LRCVIVAVDGSEESMGALRWA ENLKLRS+APDS DGTF+ LHVQ PP+IAAGLSP
Sbjct: 1   MSADLRCVIVAVDGSEESMGALRWAFENLKLRSSAPDSNDGTFIVLHVQPPPTIAAGLSP 60

Query: 61  GPIPFGGPSDLEVPAFAAAIESHQRRITAAIFDHASRICSEYKVNVETKVVIGDPKEKIC 120
           GPIPFGGPSDLEVPAF AAIESHQRRITAAI DHASRICSEYKVNVETKVVIGDPKEKIC
Sbjct: 61  GPIPFGGPSDLEVPAFTAAIESHQRRITAAILDHASRICSEYKVNVETKVVIGDPKEKIC 120

Query: 121 EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSAS 174
           E AENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGS++
Sbjct: 121 EAAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSSA 173

BLAST of MS001160 vs. ExPASy TrEMBL
Match: A0A6J1KAZ9 (universal stress protein PHOS34 OS=Cucurbita maxima OX=3661 GN=LOC111492229 PE=4 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 5.2e-85
Identity = 159/173 (91.91%), Postives = 165/173 (95.38%), Query Frame = 0

Query: 1   MSENLRCVIVAVDGSEESMGALRWALENLKLRSAAPDSTDGTFVALHVQSPPSIAAGLSP 60
           MS +LRCVIVAVDGSEESMGALRWA ENLKLRS+AP STDGTFV LHVQ PP+IAAGLSP
Sbjct: 1   MSADLRCVIVAVDGSEESMGALRWAFENLKLRSSAPGSTDGTFVVLHVQPPPTIAAGLSP 60

Query: 61  GPIPFGGPSDLEVPAFAAAIESHQRRITAAIFDHASRICSEYKVNVETKVVIGDPKEKIC 120
           GPIPFGGPSDLEVPAF AAIESHQRRITAAI  HASRICS+YKVNVETKVVIGDPKEKIC
Sbjct: 61  GPIPFGGPSDLEVPAFTAAIESHQRRITAAILGHASRICSDYKVNVETKVVIGDPKEKIC 120

Query: 121 EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSAS 174
           EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGS++
Sbjct: 121 EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSSA 173

BLAST of MS001160 vs. ExPASy TrEMBL
Match: A0A0A0LLD9 (Usp domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G371090 PE=4 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 6.3e-83
Identity = 154/173 (89.02%), Postives = 166/173 (95.95%), Query Frame = 0

Query: 1   MSENLRCVIVAVDGSEESMGALRWALENLKLRSAAPDSTDGTFVALHVQSPPSIAAGLSP 60
           MS +LRCVIVAVDGS+ESMGALRWAL+NLKL S++PDSTDGTFVALHVQ PPSIAAGLSP
Sbjct: 1   MSSDLRCVIVAVDGSDESMGALRWALQNLKLHSSSPDSTDGTFVALHVQPPPSIAAGLSP 60

Query: 61  GPIPFGGPSDLEVPAFAAAIESHQRRITAAIFDHASRICSEYKVNVETKVVIGDPKEKIC 120
            PIPFGGPSDLEVPAF AAIESHQRRITAAI +HAS+ICSEY+V VETKVVIGDPKEKIC
Sbjct: 61  DPIPFGGPSDLEVPAFTAAIESHQRRITAAILEHASKICSEYQVKVETKVVIGDPKEKIC 120

Query: 121 EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSAS 174
           EVAE+LHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDK++GS+S
Sbjct: 121 EVAEHLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKEKGSSS 173

BLAST of MS001160 vs. ExPASy TrEMBL
Match: A0A1S3BBJ3 (universal stress protein Sll1388 OS=Cucumis melo OX=3656 GN=LOC103488134 PE=4 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.8e-82
Identity = 155/173 (89.60%), Postives = 162/173 (93.64%), Query Frame = 0

Query: 1   MSENLRCVIVAVDGSEESMGALRWALENLKLRSAAPDSTDGTFVALHVQSPPSIAAGLSP 60
           MS +LRCVIVAVDGSEESM ALRWA +NLKL S+ PDSTDGTFV LHVQ PPSIAAGLSP
Sbjct: 1   MSADLRCVIVAVDGSEESMDALRWAFQNLKLCSSGPDSTDGTFVVLHVQPPPSIAAGLSP 60

Query: 61  GPIPFGGPSDLEVPAFAAAIESHQRRITAAIFDHASRICSEYKVNVETKVVIGDPKEKIC 120
            PIPFGGPSDLEVPAF AAIESHQRRITAAI +HASRICSEY+V VETKVVIGDPKEKIC
Sbjct: 61  APIPFGGPSDLEVPAFTAAIESHQRRITAAILEHASRICSEYQVKVETKVVIGDPKEKIC 120

Query: 121 EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSAS 174
           EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKK+GS+S
Sbjct: 121 EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKKGSSS 173

BLAST of MS001160 vs. TAIR 10
Match: AT1G09740.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 249.2 bits (635), Expect = 2.4e-66
Identity = 123/172 (71.51%), Postives = 142/172 (82.56%), Query Frame = 0

Query: 1   MSENLRCVIVAVDGSEESMGALRWALENLKLRSAAPDSTDGTFVALHVQSPPSIAAGLSP 60
           +S +L CV+VAVDGSE SM ALRWAL+NLKL S+   S+D +FV LHVQ  PS+AAG+SP
Sbjct: 3   VSGSLNCVVVAVDGSEVSMEALRWALDNLKLSSS---SSDSSFVVLHVQPSPSVAAGVSP 62

Query: 61  GPIPFGGPSDLEVPAFAAAIESHQRRITAAIFDHASRICSEYKVNVETKVVIGDPKEKIC 120
           G IPFGGPS LEVPAF AAIE HQ+RIT  I +HAS+IC+E  VNV+T+VVIGDPK KIC
Sbjct: 63  GTIPFGGPSGLEVPAFTAAIEQHQKRITDTILEHASQICAEKSVNVKTQVVIGDPKYKIC 122

Query: 121 EVAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSA 173
           E  ENLHADLLVMGSRA+G IKRMFLGSVSNYCTNH  CPV+I+K K+  SA
Sbjct: 123 EAVENLHADLLVMGSRAYGRIKRMFLGSVSNYCTNHAHCPVVIIKPKEDSSA 171

BLAST of MS001160 vs. TAIR 10
Match: AT3G11930.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 129.0 bits (323), Expect = 3.6e-30
Identity = 66/164 (40.24%), Postives = 98/164 (59.76%), Query Frame = 0

Query: 8   VIVAVDGSEESMGALRWALE---NLKLRSAAPDSTDGTFVALHVQSPPSIAAGLSPGPIP 67
           ++VA+D S+ S  AL+W ++   NL L +AA ++  G    +HVQSP +  A    GP  
Sbjct: 35  MVVAIDESDSSFYALQWVIDHFSNLLLTTAAAEAESGMLTVIHVQSPFNHFAAFPAGP-- 94

Query: 68  FGGPSDLEVPAFAAAIESHQRRITAAIFDHASRICSEYKVNVETKVVIGDPKEKICEVAE 127
            GG +     +   +++  Q+  +AA+   A ++C   ++  ET V+ G+ KE ICE  E
Sbjct: 95  -GGATVYASSSMIESVKKAQQETSAALLSRALQMCRAKQIRTETLVLEGEAKEMICEAVE 154

Query: 128 NLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKK 169
            +H DLLV+GSR  G IKR FLGSVS+YC +H  CP++IVK  K
Sbjct: 155 KMHVDLLVVGSRGLGKIKRAFLGSVSDYCAHHANCPILIVKPPK 195

BLAST of MS001160 vs. TAIR 10
Match: AT3G11930.4 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 127.5 bits (319), Expect = 1.1e-29
Identity = 70/167 (41.92%), Postives = 100/167 (59.88%), Query Frame = 0

Query: 8   VIVAVDGSEESMGALRWALE---NLKLRSAAPDSTDGTFVALHVQSPPSIAAGLSPGPIP 67
           ++VA+D S+ S  AL+W ++   NL L +AA ++  G    +HVQSP +  A    GP  
Sbjct: 35  MVVAIDESDSSFYALQWVIDHFSNLLLTTAAAEAESGMLTVIHVQSPFNHFAAFPAGP-- 94

Query: 68  FGGPSDLEVPAFAAAIES---HQRRITAAIFDHASRICSEYKVNVETKVVIGDPKEKICE 127
             G +   V A ++ IES    Q+  +AA+   A ++C   ++  ET V+ G+ KE ICE
Sbjct: 95  --GGATAAVYASSSMIESVKKAQQETSAALLSRALQMCRAKQIRTETLVLEGEAKEMICE 154

Query: 128 VAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKK 169
             E +H DLLV+GSR  G IKR FLGSVS+YC +H  CP++IVK  K
Sbjct: 155 AVEKMHVDLLVVGSRGLGKIKRAFLGSVSDYCAHHANCPILIVKPPK 197

BLAST of MS001160 vs. TAIR 10
Match: AT3G11930.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 125.9 bits (315), Expect = 3.1e-29
Identity = 71/167 (42.51%), Postives = 101/167 (60.48%), Query Frame = 0

Query: 8   VIVAVDGSEESMGALRWALE---NLKLRSAAPDSTDGTFVALHVQSPPSIAAGLSPGPIP 67
           ++VA+D S+ S  AL+W ++   NL L +AA ++  G    +HVQSP +  A    GP  
Sbjct: 35  MVVAIDESDSSFYALQWVIDHFSNLLLTTAAAEAESGMLTVIHVQSPFNHFAAFPAGP-- 94

Query: 68  FGGPSDLEVPAFAAAIES---HQRRITAAIFDHASRICSEYKVNVETKVVIGDPKEKICE 127
            GG +   V A ++ IES    Q+  +AA+   A ++C   ++  ET V+ G+ KE ICE
Sbjct: 95  -GGAT--AVYASSSMIESVKKAQQETSAALLSRALQMCRAKQIRTETLVLEGEAKEMICE 154

Query: 128 VAENLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKK 169
             E +H DLLV+GSR  G IKR FLGSVS+YC +H  CP++IVK  K
Sbjct: 155 AVEKMHVDLLVVGSRGLGKIKRAFLGSVSDYCAHHANCPILIVKPPK 196

BLAST of MS001160 vs. TAIR 10
Match: AT3G58450.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 115.9 bits (289), Expect = 3.2e-26
Identity = 68/169 (40.24%), Postives = 91/169 (53.85%), Query Frame = 0

Query: 8   VIVAVDGSEESMGALRWALENLKLRSAAPDST---DGTFVALHVQSPPSIAAGLSPGPIP 67
           V+VA+D S+ S  AL WA+++L++  +A   T    G    LHV   P+    + P    
Sbjct: 32  VMVAIDESKNSFDALEWAVDHLRVVISAEPETGQEGGLLTLLHVH--PTYLQYIYPS--- 91

Query: 68  FGGPSDLEVPAFAAAIESHQRRITAAIFDHASRICSEYKVNVETKVVIGDPKEKICEVAE 127
            GG   +  P   A  ES     T  +F  A  IC    V  ET ++ GDPKE IC+  E
Sbjct: 92  -GGTDSVPEPMRKAREES-----TTNLFTRALEICRGKMVKTETMILEGDPKEMICQAVE 151

Query: 128 NLHADLLVMGSRAFGPIKRMFLGSVSNYCTNHVECPVIIVKDKKRGSAS 174
             H DLLV+GSR  G IKR FLGSVS+YC  H +CP++IV+  +  S S
Sbjct: 152 QTHVDLLVVGSRGLGMIKRAFLGSVSDYCAQHAKCPILIVRPPRETSTS 189

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132206.15.3e-92100.00universal stress protein PHOS34 [Momordica charantia] >XP_022132207.1 universal ... [more]
KAG6598738.11.5e-8693.06Universal stress protein A-like protein, partial [Cucurbita argyrosperma subsp. ... [more]
XP_023547229.14.4e-8693.06universal stress protein PHOS34 [Cucurbita pepo subsp. pepo] >XP_023547230.1 uni... [more]
XP_022961627.11.7e-8591.91universal stress protein PHOS34-like [Cucurbita moschata] >XP_022961628.1 univer... [more]
XP_022997274.11.1e-8491.91universal stress protein PHOS34 [Cucurbita maxima] >XP_022997275.1 universal str... [more]
Match NameE-valueIdentityDescription
Q8L4N13.0e-1332.42Universal stress protein PHOS34 OS=Arabidopsis thaliana OX=3702 GN=PHOS34 PE=1 S... [more]
Q8LGG83.7e-1141.79Universal stress protein A-like protein OS=Arabidopsis thaliana OX=3702 GN=At3g0... [more]
Q8VYN98.2e-1132.76Universal stress protein PHOS32 OS=Arabidopsis thaliana OX=3702 GN=PHOS32 PE=1 S... [more]
O075522.9e-0830.49Stress response protein NhaX OS=Bacillus subtilis (strain 168) OX=224308 GN=nhaX... [more]
Q579512.5e-0735.62Universal stress protein MJ0531 OS=Methanocaldococcus jannaschii (strain ATCC 43... [more]
Match NameE-valueIdentityDescription
A0A6J1BRL92.6e-92100.00universal stress protein PHOS34 OS=Momordica charantia OX=3673 GN=LOC111005122 P... [more]
A0A6J1HCC88.0e-8691.91universal stress protein PHOS34-like OS=Cucurbita moschata OX=3662 GN=LOC1114623... [more]
A0A6J1KAZ95.2e-8591.91universal stress protein PHOS34 OS=Cucurbita maxima OX=3661 GN=LOC111492229 PE=4... [more]
A0A0A0LLD96.3e-8389.02Usp domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G371090 PE=4 SV... [more]
A0A1S3BBJ31.8e-8289.60universal stress protein Sll1388 OS=Cucumis melo OX=3656 GN=LOC103488134 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT1G09740.12.4e-6671.51Adenine nucleotide alpha hydrolases-like superfamily protein [more]
AT3G11930.13.6e-3040.24Adenine nucleotide alpha hydrolases-like superfamily protein [more]
AT3G11930.41.1e-2941.92Adenine nucleotide alpha hydrolases-like superfamily protein [more]
AT3G11930.23.1e-2942.51Adenine nucleotide alpha hydrolases-like superfamily protein [more]
AT3G58450.23.2e-2640.24Adenine nucleotide alpha hydrolases-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006015Universal stress protein A familyPRINTSPR01438UNVRSLSTRESScoord: 143..165
score: 38.61
coord: 5..23
score: 33.05
coord: 125..137
score: 42.77
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 1..173
e-value: 5.2E-41
score: 142.8
IPR006016UspAPFAMPF00582Uspcoord: 8..165
e-value: 6.2E-31
score: 107.8
NoneNo IPR availablePANTHERPTHR31964:SF122OS02G0760500 PROTEINcoord: 3..166
NoneNo IPR availablePANTHERPTHR31964ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEINcoord: 3..166
NoneNo IPR availableCDDcd00293USP_Likecoord: 8..164
e-value: 4.86174E-33
score: 112.463
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 7..167

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS001160.1MS001160.1mRNA